New strategies for evaluating imidacloprid-induced biological consequences targeted to Eisenia fetida species and the corresponding mechanisms of its toxicity.

Imidacloprid (IMI), a neonicotinoid insecticide, has a wide variety of applications in both agriculture and horticulture. As a result of it massive and repeated use, its traces remained in soil pose severe damage to soil invertebrates, particularly earthworms. Limited information is available regarding the underlying mechanisms of IMI toxicity toward earthworms at the molecular, transcriptional, and cellular levels. Here, Eisenia fetida coelomocytes and key defensive proteins were selected as targeted receptors to explore the toxic mechanisms of oxidative stress-mediated cytotoxicity, genotoxicity, and antioxidant responses induced by IMI stress and the molecular mechanisms underlying the binding of IMI and superoxide dismutase (SOD)/catalase (CAT). Results showed that IMI exposure destroyed the cell membrane integrity of earthworm cells, causing cell damage and cytotoxicity. The intracellular levels of ROS, including ·O2- and H2O2 were induced by IMI exposure, thereby triggering oxidative stress and damage. Moreover, IMI exposure attenuated the antioxidative stress responses (reduced antioxidant capacity and CAT/SOD activities) and caused deleterious effects (enhanced DNA damage, lipid peroxidation (LPO), and protein carbonylation (PCO)) through ROS-mediated oxidative stress pathway. Aberrant gene expression associated with oxidative stress and defense regulation, including CAT, CRT, MT, SOD, GST, and Hsp70 were induced after IMI exposure. Concentration-dependent conformational and structural alterations of CAT/SOD were observed when IMI binding. Also, direct binding of IMI resulted in significant inhibition of CAT/SOD activities in vitro. Molecular simulation showed that IMI preferred to bind to CAT active center through its direct binding with the key residue Tyr 357, while IMI bound more easily to the connecting cavity of two subunits away from SOD active center. In addition, hydrogen bonds and hydrophobic force are the main driving force of IMI binding with CAT/SOD. These findings have implications for comprehensive evaluation of IMI toxicity to soil eco-safety and offer novel strategies to elucidate the toxic mechanisms and pathways of IMI stress.

Deciphering survival strategies: Oxidative stress and microbial interplay in Eisenia fetida under tetracycline contamination.

Soil contamination resulting from residual antibiotics presents a pressing need to understand the survival mechanisms of soil organisms in polluted environments. This study focused on Eisenia fetida, and tetracycline stress experiments were conducted in a controlled environment using sterile artificial soil. The stress concentration ranged from 0 to 600 mg/kg, and stress cycles lasted either 10 or 30 days. The objective of this study was to assess the effects of oxidative stress and the changes in microbial communities both within and outside the earthworms. A comprehensive mathematical model was developed to elucidate the responses of organisms following exposure to stress utilizing factor analysis, grey relational analysis, and hierarchical entropy weight analysis. Under tetracycline stress, the initial stage (1-3 days) exhibited a coordinated regulation of oxidative stress and microbial communities in the soil with the assistance of CAT and GPX enzymes. The subsequent stage (4-5 days) further emphasized the influence of soil microbial communities. A notable "feedback regulation" of soil microbial communities on oxidative stress was observed during the third stage (6-8 days). Earthworms maintained a metabolic balance in the fourth stage (9-10 days). In the long term, the stress-induced a self-detoxification mechanism within soil microbial communities, which collaborated with GPX to respond to oxidative stress.

Gut toxicity of polystyrene microplastics and polychlorinated biphenyls to Eisenia fetida: Single and co-exposure effects with a focus on links between gut bacteria and bacterial translocation stemming from gut barrier damage.

Microplastics' (MPs) ability to sorb and transport polychlorinated biphenyls (PCBs) in soil ecosystems warrants significant attention. Although organisms mainly encounter pollutants through the gut, the combined pollution impact of MPs and PCBs on soil fauna gut toxicity remains incompletely understood. Consequently, this study examined the gut toxicity of polystyrene MPs (PS-MPs) and PCB126 on Eisenia fetida, emphasizing the links between gut bacteria and bacterial translocation instigated by gut barrier impairment. Our findings underscored that E. fetida could ingest PS-MPs, which mitigated the PCB126 accumulation in E. fetida by 9.43 %. Exposure to PCB126 inhibited the expression of gut tight junction (TJ) protein genes. Although the presence of PS-MPs attenuated this suppression, it didn't alleviate gut barrier damage and bacterial translocation in the co-exposure group. This group demonstrated a significantly increased level of gut bacterial load (BLT, ANOVA, p = 0.005 vs control group) and lipopolysaccharide-binding protein (LBP, ANOVA, all p < 0.001 vs control, PCB, and PS groups), both of which displayed significant positive correlations with antibacterial defense. Furthermore, exposure to PS-MPs and PCB126, particularly within the co-exposure group, results in a marked decline in the dispersal ability of gut bacteria. This leads to dysbiosis (Adonis, R2 = 0.294, p = 0.001), with remarkable signature taxa such as Janthinobacterium, Microbacterium and Pseudomonas, being implicated in gut barrier dysfunction. This research illuminates the mechanism of gut toxicity induced by PS-MPs and PCB126 combined pollution in earthworms, providing novel insights for the ecological risk assessment of soil.

Stereoselective bioaccumulation and dissipation of four stereoisomers of cyproconazole in earthworm-soil microcosm.

Cyproconazole is a representative and widely used triazole fungicide with four stereoisomers, which will bring some risks to non-target organisms. A fast analytical method on supercritical fluid chromatography-tandem mass spectrometry was established in 4 min, and the environmental hazards of chiral cyproconazole were studied in earthworm-soil microcosm, including stereoselective bioaccumulation and dissipation. In the process of bioaccumulation, the concentrations of cyproconazole stereoisomers in earthworms showed a trend of increasing first and then reaching a stable state at 6 mg/kg treatment, which was different from those at 0.6 mg/kg treatment (decease-increase-equilibrium). The concentration order was (2S,3R)- > (2S,3S)- > (2R,3R)- > (2R,3S)-cyproconazole and (2S,3S)- ≈ (2S,3R)- > (2R,3R)- > (2R,3S)-cyproconazole at 6 and 0.6 mg/kg treatments, respectively. The bioaccumulation factor (BAF) values were in the range of 0.018-0.55, showing weakly relative accumulation capacity. The dissipation of cyproconazole stereoisomers in artificial soil accorded with the first-order kinetics equation, and the half-lives were 20.1-23.6 and 7.66-8.28 days at 6 and 0.6 mg/kg treatments, respectively, without stereoselectivity and diastereoselectivity. In earthworms, the dissipation half-lives were 5.81-6.01 days with the preferential dissipation of (2R,3R)-cyproconazole. The study would help with the rational uses and risk assessments of cyproconazole.

Integrated microbiota and multi-omics analysis reveal the differential responses of earthworm to conventional and biodegradable microplastics in soil under biogas slurry irrigation.

As one of the promising alternatives of conventional plastic mulching film (C-PMF), biodegradable plastic mulching films (B-PMF) were employed in agronomy production to alleviate the environmental burden of C-PMF. However, information regarding the potential toxicity effects of biodegradable microplastics (MPs) in soil still in scarcity, and the available findings were found to be controversial. Additionally, little is known about the molecular toxicity effects of conventional and biodegradable MPs on terrestrial organisms. Thus, 5 % (w/w) biodegradable (polylactic acid, PLA) and conventional (polyvinylchloride, PVC; low-density polyvinylchloride, LDPE) MPs were employed to assess the toxicity effects on Eisenia fetida in agricultural soil with biogas slurry irrigation. In the present study, transcriptomic, metabolomic profiles and individual indexes were selected to reveal the toxicity mechanisms from molecular level to the individual response. Furthermore, dysbiosis of bacterial community in gut was also investigated for obtaining comprehensive knowledge on the MPs toxicity. At the end of the exposure, the number of survival earthworms after MPs exposure was significantly reduced. Compared with the initial body weight, PLA and LDPE increased the biomass of earthworms after MPs exposure, while no significant influence on the biomass was observed in PVC treatment. Microbacterium, Klebsiella and Chryseobacterium were significantly enriched in earthworm gut after PLA, PVC and LDPE exposure, respectively (p < 0.05). Transcriptomic and metabolomic analysis revealed that PLA exposure induced neurotransmission disorder and high energetic expenditure in earthworms. However, PVC and LDPE inhibited the nutrient absorption efficiency and activated the innate immunity responses of earthworms. The PLS-SEM results showed that the effects of MPs were dominated by the polymer types, and hence, significantly and directly influence the gut bacterial community of earthworms. This study provides a better understanding of the similarities and discrepancies in toxicity effects of biodegradable and conventional MPs from the perspectives of individual, gut bacterial community, transcriptome and metabolome.

Enhancing earthworm (Lumbricus terrestris) tolerance to plastic contamination through gut microbiome fortification with plastic-degrading microorganisms.

Microorganisms from L. terrestris gut previously exposed to different types of plastic (PET, LDPE, LLDPE, and PS) were studied to be used as probiotics of earthworms in plastic-contaminated soils (LDPE, LLDPE and recycled mulching film) at mesocosm-scale trials. The most abundant morphotypes with enzymatic capacities of interest were identified. Pseudomonas alkylphenolica (PL4) and Pseudomonas putida (PL5) strains were selected to be used as inoculants using Morus alba leaves as carriers to strengthen the intestinal microbiota of earthworms. Culture (selective cetrimide agar medium) and molecular (qPCR) techniques were used to trace the presence of the inoculum in the intestine of the earthworms. Additionally, a metataxonomic analysis was carried out to study the biodiversity and functionality of the earthworm microbiome, and their measure of survival and weight. Probiotics improved the survival rates of earthworms exposed to plastics, which also increased the abundance of microbial groups of interest in plastic bioremediation tasks.

Effects of artificial sweetener acesulfame on soil-dwelling earthworms (Eisenia fetida) and its gut microbiota.

Artificial sweeteners (AS) are the emerging contaminants with potential toxicity to living organisms. The effects of AS to soil typical invertebrates have not been revealed. In this study, the responses of earthworms (Eisenia fetida) and gut microbial communities to acesulfame-contaminated soils (0.1, 1 and 10 mg kg-1) were studied using transcriptomics, metabolomics and metagenomics analyses. The fresh weight of earthworms was significantly stimulated by acesulfame at concentrations of 1 mg kg-1. Sphingolipid metabolism, purine metabolism, cutin, suberine and wax biosynthesis pathways were significantly affected. At 10 mg kg-1 treatment, the amount and weight of cocoons were significantly increased and decreased, respectively, accompanied by the significant disorder of ECM-receptor interaction, and carbon fixation in photosynthetic organisms pathways. Lysosome pathway was significantly affected in all the treatments. Moreover, the acesulfame significantly increased the relative abundance of Bacteroidetes and Mucoromycota, and decreased Proteobacteria in the gut of earthworms. Our multi-level investigation indicated that AS at a relatively low concentration induced toxicity to earthworms and AS pollution has significant environmental risks for soil fauna.

Toxicological approaches as tool to assess the effects of a mixture of photocatalytic degradation products originated from the unregulated neonicotinoid acetamiprid employing a terrestrial organism (Eisenia andrei).

Acetamiprid (ACT) has been detected in several water sources in Latin America. The presence of its degradation products in the environment is not negligible and transformation products (TPs) significantly contribute to environmental health risks. Although advanced oxidative processes are promising for the treatment of this neocotinoid, effects of these are still unknown. In this context, the effects of a mixture of photocatalytic degradation products resulting from an ACT treatment for 90 min employing TiO2/UV on cytotoxicity and oxidative stress parameters in Eisenia andrei earthworms in acute and chronic experiments using typical Latin American soil were assessed. Acute contact tests were performed (72 h) using a filter paper moistened with an ACT solution and a chronic test was performed using Oxisoil (200 g) moistened with an ACT solution for 45 days. Catalase (CAT) and glutathione-S-transferase (GST) activities, reduced glutathione (GSH) levels and cytotoxicity (cellular eleocyte and amoebocyte assessments) were investigated. Over 75 % of ACT was degraded within the first 15 min of treatment, with levels below the limit of detection after 60 min. The acute test revealed greater cytotoxic effects associated with the effluents treated for T0 and T15 min, with decreased cell density noted after 48 h of exposure, in addition to CAT induction (in all treatments) and GST induction following T0, T15 and T90 min exposures. Concerning the chronic assay, decreases in cell density (T0, T15, T60 and T90 min) and viability (T0, T60 and T90 min) were observed after 45 days, in addition to induced CAT activity following T0, T15 and T60 exposures and GST induction following the T60 min exposure. Reduced glutathione levels were unaltered, comprising the least sensitive biomarker among the investigated parameters to the treated effluent exposures. The mixture of ACT degradation products can cause toxic effects to non-target organisms, despite parent compound degradation, alerting for the need for ecotoxicological tests to prove decreased effluent toxicity, in addition to the improvement of degradation techniques.

Toxicity of carbon nanofibers in earthworms (Lumbricus terrestris) naturally infected with Monocystis sp.

Although the ecotoxicity of carbon-based nanomaterials (CBNs) is known, the potential effect of carbon nanofibers (CNFs) on edaphic organisms has been insufficiently explored. Thus, we aimed at the ecotoxicity of CNFs (at 10 and 100 mg/kg) in Lumbricus terrestris earthworms naturally infected with Monocystis sp. After 28 days of exposure, treatments did not affect the survival rate. However, we observed a significant loss of body biomass, and Monocystis sp. infection in seminal vesicles was potentiated by exposure to CNFs. Earthworms exposed to CNFs showed a redox imbalance in the seminal vesicle, muscle, and intestine and an alteration in nitric oxide production in these organs. In muscles, we also noticed a significant reduction in AChE activity in earthworms exposed to CNFs. The histopathological analyses revealed the treatments' significant effect on the structures of the different evaluated tissues. Although we did not notice a concentration-response for several of the biomarkers, when taken together and after the application of Integrated Biomarker Response (IBR) and principal component analysis (PCA), we noticed that the response of earthworms to CNFs at 100 mg/kg showed a more significant deviation from the unexposed group. This was mainly determined by inhibiting antioxidant activity in the seminal vesicle, biochemical biomarkers assessed in muscle and intestine, and histomorphometric muscle biomarkers from earthworms exposed to CNFs at 100 mg/kg. Thus, we demonstrate that CNFs increase the parasite load of Monocystis sp. of adult L. terrestris earthworms and induce biochemical and histopathological changes, especially at 100 mg/kg. Our results point to the additional impact these nanomaterials can have on the health of earthworms, signaling the need for greater attention to their disposal and ecotoxicological effects on soil organisms.

Molecular transformation pathway and bioavailability of organic phosphorus in sewage sludge under vermicomposting.

Phosphorus reclamation from sewage sludge is essential for sustainable phosphorus management, as large quantities of phosphorus afflux into wastewater treatment plants and are finally enriched in sewage sludge via phosphorus removal technologies. Meanwhile, vermicomposting is a cost-effective biotechnique for sludge stabilization. This work unveiled the molecular transformation pathway and bioavailability of organic phosphorus (OP) in sludge under vermicomposting with solution 31P NMR, FT-ICR MS and enzymatic hydrolysis assay. In conclusion, vermicomposting transformed OP in two stages. In stage I (day 0 to 14), macromolecule CHONP such as phospholipids, phosphoproteins and nucleic acid were decomposed into orthophosphate and high bioavailability OP including flavin mononucleotide, flavin mononucleotide hydrate and N6-isopentenyladenosine 5'-monophosphate under the action of earthworm intestinal flora. This resulted in the bioavailability of OP reaching a maximum of 13.58 mg/L on day 14. In stage II (day 14 to 28), the enzyme in vermicompost began to dominate the transformation of OP. Under the catalysis of phosphate, high bioavailability orthophosphate monoester was decomposed into orthophosphate. Nitrogen-containing aromatic OP polymerization produced humic acid-like OP under the catalysis of ligase. And phytic acid-like OP were produced under the catalysis of transferase. These led to the OP bioavailability decreasing to 5.60 mg/L on day 28. This work provides a new perspective on sludge phosphorus recovery and use.

Co-exposure to UV-aged microplastics and cadmium induces intestinal toxicity and metabolic responses in earthworms.

Aged microplastics (MPs) alter the interaction with heavy metals due to changes in surface properties. However, the combined toxicological effects of aged MPs on heavy metals in soil remain poorly understood. In this study, earthworms were employed as model animals to investigate the effects of aged MPs on the biotoxicity of cadmium (Cd) by simulating the exposure patterns of original and UV-aged MPs (polylactic acid (PLA) and polyethylene (PE)) with Cd. The results showed that UV-aging decreased the zeta potential and increased the specific surface area of the MPs, which enhanced the bioaccumulation of Cd and caused more severe oxidative stress to earthworms. Meanwhile, the earthworm intestines exhibited increased tissue damage, including chloragogenous tissue congestion lesions, and typhlosole damage. Furthermore, the combined exposure to UV-aged MPs and Cd enhanced the complexity of the microbial network in the earthworm gut and interfered with endocrine disruption, membrane structure, and energy metabolic pathways in earthworms. The results emphasized the need to consider the degradation of MPs in the environment. Hence, we recommend that future toxicological studies use aged MPs that are more representative of the actual environmental conditions, with the results being important for the risk assessment and management of MPs.

Isolation, identification of antioxidant peptides from earthworm proteins and analysis of the structure-activity relationship of the peptides based on quantum chemical calculations.

Earthworms are emerging sources of edible animal proteins. Earthworm extracts exhibit good in vivo antioxidant activity after oral administration. To better understand the antioxidant activity of earthworms, antioxidant peptides derived from earthworm proteins after gastrointestinal digestion were isolated and identified, and their structure-activity relationships were analysed in this research. Results showed that earthworm protein gastrointestinal digestion products exhibited good antioxidant activity, and 6030 peptide sequences were identified after separation using ion-exchange and gel-chromatography columns. Eleven peptides were screened using computer simulation activity scores, among which AFWYGLPCKL, WPWQMSLY, and GCFRYACGAFY showed the best antioxidant activities. Highest Occupied Molecular Orbital (HOMO) analysis indicated that N29-H10, O122-H38, and the peptide bond binding sites of serine and leucine were active sites of peptides AFWYGLPCKL, GCFRYACGAFY, and WPWQMSLY, respectively. This study provides a new understanding of substance basis of antioxidant activity in earthworms and contributes to application of earthworm proteins as antioxidants in health-foods.

Pesticide effect on earthworm lethality via interpretable machine learning.

Earthworms are among the most important animals (invertebrates) for soil health. Many chemical substances released into nature for agricultural development, such as pesticides, may have unwanted effects on those organisms. However, it is essential to understand the extent of the impact of chemicals on soil health first and then make the proper decisions for regulatory or commercial purposes. We hypothesize that there is an expressible quantitative structure-activity relationship (QSAR) between the structure of pesticide compounds and the acute toxicity effect of earthworm species Eisenia fetida. The description of this relationship allows for a better assessment of the impact of chemicals on the said earthworm. To describe this relationship, a dataset of chemicals was collected from open-access sources to develop a mathematical model. A novel approach, combining genetic algorithm and Bayesian optimization, was used to select structural features into the model and to optimize model parameters. The final QSAR classification model was created with the Random Forest algorithm and exhibited good prediction Accuracy of 0.78 on training set and 0.80 on test set. The model representation follows FAIR principles and is available on QsarDB.org.

Network complexity of bacterial community driving antibiotic resistome in the microbiome of earthworm guts under different land use patterns.

Recently, the study of antibiotic resistance in the soil animal microbiome has attracted extensive attention; however, the patterns of antibiotic resistance genes (ARGs) in soil and soil animals related to different land use types remain poorly studied. In the present study, soil and earthworms were collected from four different land-use types (farmland, hospital, park land, and mountain park), and 162 ARGs in the microbiomes of the soil and earthworms were quantified using high-throughput quantitative PCR. Our study showed that the abundance and number of ARGs were higher in soil samples than in earthworm guts, but earthworms as the living organisms created relatively isolated ambient surroundings, which allowed for a more heterogeneous ARGs profile. Meanwhile, land use significantly influenced the abundance, number and co-occurrence pattern of ARGs in the soil and earthworm samples. Furthermore, abiotic and biotic factors had significant effects on the ARGs profile, among which pH had a negative effect on the ARGs profiles of both soil and earthworm microbiomes, and bacterial network complexity had a positive effect on the earthworm ARGs profile. Our study provides new insights into the distribution and dispersal of ARGs in the soil animal gut microbiome under different land use patterns.

The Effects of Lumbricus rubellus Extract on Staphylococcus aureus Colonization and IL-31 Levels in Children with Atopic Dermatitis.

Background and Objectives: The ineffective combination of corticosteroids and antibiotics in treating some atopic dermatitis (AD) cases has been concerning. The skin barrier defects in AD ease the colonization of Staphylococcus aureus (S. aureus), which results in a rise in interleukin-31 (IL-31). Lumbricus rubellus (L. rubellus) has shown antimicrobial and antiallergic effects but has not been studied yet to decrease the growth of S. aureus and IL-31 levels in AD patients. This study aimed to analyze the effect of L. rubellus extract in reducing S. aureus colonization, the IL-31 level, and the severity of AD. Materials and Methods: A randomized controlled trial (RCT) (international registration number TCTR20231025004) was conducted on 40 AD patients attending Dermatology and Venereology Polyclinic, Mother and Child Hospital (RSIA), Aceh, Indonesia, from October 2021 to March 2022. AD patients aged 8-16 who had a Scoring Atopic Dermatitis (SCORAD) index > 25, with total IgE serum level > 100 IU/mL, and had healthy weight were randomly assigned into two groups: one received fluocinolone acetonide 0.025% and placebo (control group) and one received fluocinolone acetonide 0.025% combined with L. rubellus extract (Vermint®) (intervention group). The S. aureus colony was identified using a catalase test, coagulase test, and MSA media. The serum IL-31 levels were measured using ELISA assay, while the SCORAD index was used to assess the severity of and improvement in AD. Mean scores for measured variables were compared between the two groups using an unpaired t-test and Mann-Whitney U test. Results: A significant decline in S. aureus colonization (p = 0.001) and IL-31 (p = 0.013) in patients receiving L. rubellus extract was found in this study. Moreover, fourteen AD patients in the intervention group showed an improvement in the SCORAD index of more than 35% (p = 0.057). Conclusions: L. rubellus extract significantly decreases S. aureus colonization and the IL-31 level in AD patients, suggesting its potential as an adjuvant therapy for children with AD.

Physiological and behavioral assessment of Metaphire posthuma in response to clothianidin insecticide: Insights from molecular and biochemical analysis.

In the present study, Clothianidin [(E) - 1-(2 - chloro-1,3 - thiazol - 5-ylmethyl) - 3-methyl - 2- nitroguanidine] (CLO) was selected as a soil pollutant and earthworm was employed as a test organism. The various responses like biochemical and detoxification process of earthworm Metaphire posthuma towards Clothianidin at lethal and sublethal doses were studied using OECD-standardized toxicological guidelines. The present study examined the toxicity of CLO to earthworms after 28 days of exposure at conc. 0, 1.5, 3, 6, 12 and 24 mg kg-1 in a soil mixture. Biochemical markers including Guaiacol peroxidase (POD), Superoxide dismutase (SOD), Catalase (CAT), Glutathione S-transferase (GST) and content of Malondialdehyde (MDA) in earthworms were measured. Acute toxicity tests revealed that CLO caused a concentration-dependent increase in mortality with LC50 (Lethal concentration) values of 10.960 and 8.201 mg kg-1 for 7th and 14th day respectively. The earthworms were exposed to CLO contaminated soil for 56 days and reflecting the significant decrease in earthworm growth, cocoon and hatchling production. Moreover, enzyme activities such as CAT, SOD, POD and MDA content were significantly enhanced with the increased concentration and exposure period of CLO. Molecular docking studies indicated that CLO primarily interacts to the junction site of SOD and in active centres of CAT, POD and GST. As a result, the current findings imply that the sub chronic CLO exposure can induce variations in physiology and avoidance behaviour of earthworms, oxidative stress as well as alterations in enzyme activities.

Structure and diversity of earthworm communities in long-term irrigated soils with raw effluent and treated wastewater.

This study was conducted in two natural meadows: first, soils were irrigated with raw wastewater (SIRWW) and in the second, soils were irrigated with treated wastewater (SITWW). Earthworms were sampled in eight soil blocks spaced 10 m apart at each site. Earthworm community was characterized and compared using density, biomass, composition, structure, species richness, and diversity parameters. At both meadows, 459 earthworm individuals from two families and seven species were collected. The highest earthworm density and species richness were recorded at SIRWW. Nicodrilus caligenus was the most abundant species. Most of earthworm community parameters decreased significantly at SITWW. Only two species (N. caligenus and Octodrilus complanatus) were common between the two grasslands. Among the seven species identified at both meadows, four (Allolobophora longa, Eisenia foetida, Allolobophora rosea, Allolobophora chlorotica) were exclusively present in SIRWW, whereas a single species (Amynthas sp.) was characterized in SITWW. Three ecological earthworm groups (epigeic, endogeic, and anectic) were represented in SIRWW, with the dominance of endogeics. Further studies are needed to quantify pollution in this soils and the accumulation of pollutant load in earthworms. It is also important to highlight the relationship between the abundance and diversity of earthworms in these two ecosystems with soil biological activity.

Multimodal steerable earthworm-inspired soft robot based on vacuum and positive pressure powered pneumatic actuators.

This article presents a multimodal steerable earthworm-inspired soft robot based on vacuum and positive pressure powered pneumatic actuators capable of crawling both inside pipelines and on planar surfaces. The optimized modular vacuum pressure-driven actuator can generate deformation and anchoring motion through a unified structure under low vacuum pressure, giving it significant speed advantages and multi-modal locomotion capabilities. Meanwhile, the positive pressure powered actuator (PPPA) enables the robot to achieve controlled multi-directional and multi-degrees-of-freedom steering, moreover, enhances the consistency of the driving mechanism. The incorporation of front-end pressure sensing enables the robot to autonomously detect and evaluate pressure, facilitating automatic obstacle avoidance through the activation of corresponding turning units of PPPA. In the process of optimizing motion parameters, the overall motion efficiency has been improved by 16.7% by improving the control law. Through adjustments and optimizations of the interval time (cycle time), the robot is able to achieve a speed of 7.16 mm s-1during planar locomotion and 1.94 mm s-1during in-pipe locomotion. Using the developed robot, we conducted a series of turning experiments, including surface obstacle avoidance and cross-plane crawling, which demonstrated its enhanced capability in cross-plane steering and locomotion. Its related speed indicators showcase superior overall performance compared to other developed robots of the same type.

Prediction of perfluoroalkyl acids (PFAAs) in homegrown eggs: Insights into abiotic and biotic factors affecting bioavailability and derivation of potential remediation measures.

Homegrown eggs from free-ranging laying hens often contain elevated concentrations of perfluoroalkyl acids (PFAAs). However, it is unclear which factors contribute to these relatively large exposure risk scenarios. Moreover, existing bioavailability and modeling concepts of conventional organic pollutants cannot be generalized to PFAAs due to their different physicochemical soil interactions. Therefore, there is an urgent need for empirical models, based on real-world data, to provide insights into how (a)biotic factors affect the bioavailability to eggs. To this end, 17 targeted analytes were analyzed in abiotic (i.e. rainwater, soil; both N = 101) matrices and homegrown eggs (N = 101), which were sampled in 101 private gardens across Flanders (Belgium) in 2019, 2021 and 2022. Various soil characteristics were measured to evaluate their role in affecting PFAA bioavailability to the eggs. Finally, PFAAs were measured in potential feed sources (i.e. homegrown vegetable and earthworm pools; respectively N = 49 and N = 34) of the laying hens to evaluate their contribution to the egg burden. Modeling suggested that soil was a major exposure source to laying hens, accounting for 16-55% of the total variation in egg concentrations for dominant PFAAs. Moreover, concentrations in vegetables and earthworms for PFBA and PFOS, respectively, were significantly positively related with corresponding egg concentrations. Predictive models based on soil concentrations, total organic carbon (TOC), pH, clay content and exchangeable cations were successfully developed for major PFAAs, providing possibilities for time- and cost-effective risk assessment of PFAAs in homegrown eggs. Among other soil characteristics, TOC and clay content were related with lower and higher egg concentrations for most PFAAs, respectively. This suggests that bioavailability of PFAAs to the eggs is driven by complex physicochemical interactions of PFAAs with TOC and clay. Finally, remediation measures were formulated that are readily applicable to lower PFAA exposure via homegrown eggs.

Potential ecotoxicological effects of silver nanoparticles and silver sulphide on the endogeic earthworm Aporrectodea caliginosa (Savigny 1826).

Silver nanoparticles (AgNPs) are increasingly used in consumer products and subsequently arrive in wastewater systems, accumulating as silver sulphide (Ag2S) in the resulting biosolids, which are commonly spread onto agricultural fields as a fertiliser. Experiments were performed to investigate the effect of AgNPs, using the endogeic earthworm Aporrectodea caliginosa as a test organism. In an acute toxicity experiment, A. caliginosa were exposed to soil containing different concentrations of AgNPs (0, 1, 5, 10, 50, 100, 250, 500, 750, and 1000 mg kg-1 dry soil) and Ag2S (0, 10, 50, 100, 500, and 1000 mg kg-1 dry soil). Earthworm biomass and mortality were monitored. Earthworms exposed to 500, 750 and 1000 mg kg-1 fresh AgNPs had mortality rates of 20%, 60% and 70%, respectively. Changes in biomass were directly related to AgNP concentration. Exposure to Ag2S did not affect biomass or mortality. Further experiments used 0, 10, 50, 100 and 250 mg kg-1 AgNPs and 0, 50, 100, 500, and 1000 mg kg-1 Ag2S to evaluate sublethal effects on A. caliginosa. Avoidance behaviour in a linear gradient was evaluated after 14 days. Earthworms significantly preferred soil that was free of either AgNPs or Ag2S. The same concentrations were used to assess effects on cocoon production of A. caliginosa exposed to AgNPs and Ag2S. In the first 3 months of AgNP exposure, higher concentrations had a negative effect on cocoon production, but this effect diminished thereafter. Ag2S had no discernible effect on reproduction. Overall, introduction of AgNPs into the soil through the application of biosolids appears to be of low concern to the tested endogeic earthworm.