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1.
Andrologia ; 52(1): e13453, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31762071

RESUMO

miRNAs (MicroRNAs), known as noncoding and important endogenous factors regulating the expression protein-coding genes, are vital regulators in each biological process. Thus, this study aims to explore the key role of four microRNAs in regulating the spermatogenesis. To conduct this experiment, 55 infertile and fertile men provided the study with the sperm and testicular tissue samples. To study the spermatozoa in terms of the morphology, Diff-Quick was applied. Then, quantitative real-time polymerase chain reaction (RT-PCR) was conducted on samples. Our data indicated that in contrast to the miR-15b, significant increasing of miR-383 and miR-122 occurred in both severe oligoasthenoteratozoospermia (SOAT) and moderate oligoasthenoteratozoospermia (MOAT) compared to normal sperm group (N). In addition, it was observed that miR-15b and miR-122 increased in patients with nonobstructive azoospermia (NOA) compared with obstructive azoospermia (OA) group. Expression levels of target genes including P53, CASPASE-9 and CYCLIN D1 underwent principle changes according to miRNAs expression level. Our finding indicated that miRNAs had essential role in the regulation of spermatogenesis, and their expression altering was associated with sperm abnormalities. Thus, microRNAs can be introduced as useful biomarkers to determine male infertility reasons to choose the effective treatment.


Assuntos
Azoospermia/diagnóstico , Redes Reguladoras de Genes , MicroRNAs/metabolismo , Oligospermia/diagnóstico , Espermatogênese/genética , Adulto , Azoospermia/genética , Biomarcadores/análise , Biomarcadores/metabolismo , Caspase 9/genética , Ciclina D1/genética , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Humanos , Masculino , MicroRNAs/análise , Oligospermia/genética , Espermatozoides/metabolismo , Proteína Supressora de Tumor p53/genética , Adulto Jovem
2.
Andrologia ; 52(1): e13415, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31670423

RESUMO

Oligoasthenozoospermia is a common factor leading to male infertility. Acupuncture has been applied for treating male infertility for several thousand years in China, but clinical evidence of its efficacy and safety in treating oligoasthenozoospermia is yet to be established. This review aimed to systematically assess the evidence on the effect of acupuncture in males with oligoasthenozoospermia. Databases (PubMed, EMBASE, SINOMED, CNKI, Wanfang database and Cochrane Library) were searched to identify related studies published before 30th June 2019. The Cochrane risk of bias tool and Jadad score was adopted to assess the methodological quality of included studies. Twelve randomised controlled trials (RCTs) with 1,088 participants were included in this review. The aim of this study was to perform a meta-analysis, but it was not possible due to considerable clinical heterogeneity among the included studies. According to the narrative analysis, acupuncture or acupuncture combined with another intervention was effective in improving the semen quality based on the included studies. However, this result should be interpreted with caution due to high risk. The methodological quality of most included studies was low. The current evidence on acupuncture for oligoasthenozoospermia is inadequate to draw a solid conclusion due to the poor methodological quality. Rigorous full-scale RCTs are needed to validate the therapeutic efficacy and safety of acupuncture in treating oligoasthenozoospermia.


Assuntos
Terapia por Acupuntura/efeitos adversos , Astenozoospermia/terapia , Oligospermia/terapia , Astenozoospermia/diagnóstico , Humanos , Masculino , Oligospermia/diagnóstico , Ensaios Clínicos Controlados Aleatórios como Assunto , Análise do Sêmen , Resultado do Tratamento
3.
Dis Markers ; 2019: 9803498, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31885747

RESUMO

Vimentin is a structural protein predominantly located in the head of sperms. The function and localization of the previously identified truncated version, Vimentin 3 (Vim3), are still unknown. To investigate whether the expression of Vim3 can be used as a reliable marker for the differentiation of sperm quality, we analyzed ejaculates from patients with oligoasthenoteratozoospermia (OAT) syndrome and normozoospermia. We identified sperms with head, neck, and tail changes, which were less positive for Vim3 in OAT syndrome compared to normozoospermia. The expression of Vim3 was significantly downregulated in patients with OAT syndrome compared to sperms from patients with normozoospermia (∗∗ p < 0.01). The ELISA analysis showed similar results as ejaculates from normozoospermic patients showed a significantly higher Vim3 concentration than patients with OAT syndrome (∗∗∗ p < 0.001). This study demonstrates that Vim3 is more highly expressed in ejaculates from patients with normozoospermia compared to ejaculates from patients with OAT syndrome. Therefore, we postulate that Vim3 can be used to determine ejaculate quality. Furthermore, we identified the marker, Vim3, to differentiate between mature sperms with no morphological changes and sperms with head, neck, and tail changes. A lateral flow assay that allows quick analysis is currently under development.


Assuntos
Regulação para Baixo , Oligospermia/diagnóstico , Espermatozoides/metabolismo , Vimentina/metabolismo , Adulto , Processamento Alternativo , Biomarcadores/metabolismo , Estudos de Casos e Controles , Humanos , Masculino , Oligospermia/genética , Oligospermia/metabolismo , Sêmen/metabolismo , Vimentina/genética , Adulto Jovem
4.
J Assist Reprod Genet ; 36(10): 1975-1987, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31396849

RESUMO

PURPOSE: To determine the consequences of an altered sperm fluorescence in situ hybridization (FISH) result for ART outcomes and the indications for a sperm FISH analysis. METHODS: Data from 439 infertile men were collected. Bivariate analyses were performed to determine the association of men's age, seminal alterations, and sperm FISH indication, with the incidence of X, Y, 13, 18, and 21 sperm chromosomal abnormalities. A multivariate logistic regression analysis was performed to establish the most predictive variables for altered sperm FISH. Results from the IVF/ICSI cycles were collected for 248 out of 439 patients. Two distinct groups were established: 151 couples that used their own oocytes and 97 couples involved in egg donation programs. In both groups, ART outcomes were compared between normal and altered sperm FISH. RESULTS: Teratozoospermia and oligozoospermia were associated with sperm chromosome anomalies (p < 0.05). Indications for sperm FISH analysis with the highest predictability were teratozoospermia, male age, oligozoospermia, and implantation failure (AUC = 0.702). Embryo quality (p = 0.096), pregnancy rate (p = 0.054), and implantation rate (p = 0.089) were higher in own-oocytes couples with normal sperm FISH than in altered sperm FISH couples, although differences were not statistically significant. In donor-oocytes couples, in which high-quality embryos were transferred later than in own-oocytes couples (3.8 vs. 3.0 days), we did not identify differences in the ART outcome between normal and altered sperm FISH couples. In both groups, the possible interference of woman age was negligible. CONCLUSIONS: Sperm FISH is indicated in middle-aged oligoteratozoospermic patients with implantation failures in previous IVF/ICSI cycles. Sperm chromosome anomalies have a moderate detrimental impact on embryo quality, implantation, and pregnancy rates.


Assuntos
Hibridização in Situ Fluorescente , Oligospermia/diagnóstico , Espermatozoides/ultraestrutura , Teratozoospermia/diagnóstico , Adulto , Aberrações Cromossômicas , Implantação do Embrião/genética , Implantação do Embrião/fisiologia , Feminino , Fertilização In Vitro/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Oligospermia/genética , Oligospermia/patologia , Gravidez , Taxa de Gravidez , Técnicas de Reprodução Assistida , Injeções de Esperma Intracitoplásmicas/métodos , Espermatozoides/patologia , Teratozoospermia/genética , Teratozoospermia/patologia , Doadores de Tecidos
5.
Ann Endocrinol (Paris) ; 80(4): 234-239, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31439307

RESUMO

OBJECTIVE: Congenital FSH deficiency is an exceptional cause of male infertility most often attributed to FSH ß gene mutations. The few published cases report azoospermia, severe testicular hypotrophy and normal testosterone levels associated with normal virilization. We report the exploration of two young men aged 26 and 27 years with severe sperm abnormalities, moderate testicular hypotrophy and isolated FSH deficiency. METHODS: Several FSH, LH, total testosterone and inhibin B assays and FSH ß gene sequencing were performed. RESULTS: FSH was almost undetectable at baseline and poorly responsive to GnRH test, whereas LH was normal at baseline and increased after GnRH test. Testosterone levels were within the adult range, while inhibin B levels were upper-normal to high. No FSH ß gene mutations were found. Exogenous FSH treatment was followed by spontaneous pregnancy in one case and required intra-cytoplasmic sperm injection (ICSI) in the other. CONCLUSIONS: The paradoxical high levels of inhibin B reflect the presence of functional Sertoli cells and may explain the isolated FSH deficiency. An intra-gonadal factor stimulating inhibin B secretion is discussed.


Assuntos
Subunidade beta do Hormônio Folículoestimulante/genética , Hormônio Foliculoestimulante/deficiência , Infertilidade Masculina/diagnóstico , Oligospermia/diagnóstico , Adulto , Análise Mutacional de DNA , Hormônio Foliculoestimulante/genética , Humanos , Infertilidade Masculina/genética , Masculino , Mutação , Oligospermia/genética
6.
Eur Urol ; 76(5): 626-636, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31400948

RESUMO

CONTEXT: European and North American guidelines recommend Y-chromosome microdeletion (YCM) screening in azoospermic and oligozoospermic men with sperm concentrations of <5 million sperm/ml; however, numerous studies have suggested that YCMs are rare when sperm concentrations are >1 million sperm/ml. OBJECTIVE: We systematically reviewed and meta-analyzed European and North American studies to determine the prevalence of a complete YCM in oligozoospermic men with sperm concentrations of >0-1, >1-5, and >5-20 million sperm/ml, and to determine whether 1 or 5 million sperm/ml is the most appropriate sperm concentration threshold for YCM screening. EVIDENCE ACQUISITION: A systematic review of MEDLINE, EMBASE, Cochrane Library, and ClinicalTrials.gov was performed for studies assessing the prevalence of a complete YCM in oligozoospermic men in European and North American studies. EVIDENCE SYNTHESIS: Thirty-seven studies were identified during a systematic review (n = 12 492 oligozoospermic men). All complete YCMs in oligozoospermic men were AZFc microdeletions. Eighteen studies contained data conducive to meta-analysis (n = 10 866 men). Comparing the pooled estimated prevalence by sperm concentration, complete YCMs were significantly more common in men with sperm concentrations of >0-1 million sperm/ml (5.0% [95% confidence interval {CI}: 3.6-6.8%]) versus >1-5 million sperm/ml (0.8% [95% CI: 0.5-1.3%], p < 0.001). YCMs were similar in men with sperm concentrations of >1-5 and >5-20 million sperm/ml (0.8% [95% CI: 0.5-1.3%] vs 0.5% [95% CI: 0.2-0.9%], p = 0.14). CONCLUSIONS: In Europe and North America, the majority of YCMs occur in men with sperm concentrations of ≤1 million sperm/ml, with <1% identified in men with >1 million sperm/ml. Male infertility guidelines for North America and Europe should reconsider the sperm concentration screening thresholds to recommend testing for YCMs only for men with sperm concentrations of <1 million sperm/ml. PATIENT SUMMARY: Complete Y-chromosome microdeletions (YCMs) are rare in men with >1 million sperm/ml. Routine screening for YCMs should occur only if sperm concentration is ≤1 million sperm/ml.


Assuntos
Testes Genéticos , Infertilidade Masculina/diagnóstico , Oligospermia , Transtornos do Cromossomo Sexual no Desenvolvimento Sexual/diagnóstico , Contagem de Espermatozoides , Deleção Cromossômica , Cromossomos Humanos Y , Europa (Continente)/epidemiologia , Testes Genéticos/métodos , Testes Genéticos/normas , Humanos , Masculino , Programas de Rastreamento/métodos , Programas de Rastreamento/normas , América do Norte/epidemiologia , Oligospermia/diagnóstico , Oligospermia/epidemiologia , Oligospermia/genética , Prevalência , Aberrações dos Cromossomos Sexuais , Contagem de Espermatozoides/métodos , Contagem de Espermatozoides/estatística & dados numéricos
7.
Fertil Steril ; 112(1): 61-72.e1, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31103287

RESUMO

OBJECTIVE: To study peripheral blood DNA differential methylation in oligozoospermic infertile men in comparison with normozoospermic fertile controls. DESIGN: Case-control study. SETTING: Reproductive biology laboratory. PATIENTS(S): Azoospermic and oligozoospermic infertile patients (n = 6) and normozoospermic fertile controls (n = 6) in the discovery phase, and oligo/asthenozoospermic infertile men (n = 11) and normozoospermic fertile controls (n = 10) in the validation phase. INTERVENTION(S): Blood samples drawn from all participants, DNA isolation and methylation analysis. MAIN OUTCOME MEASURE(S): DNA methylation values analyzed using genomewide methylation 450K BeadChip array, followed by deep sequencing of selected regions for methylation analysis in the neighborhood regions of differentially methylated CpGs. RESULT(S): We found 329 differentially methylated CpG spots, out of which 245 referred to the genes, representing 170 genes. Deep-sequencing analysis confirmed the methylation pattern suggested by 450K array. A thorough literature search suggested that 38 genes play roles in spermatogenesis (PDHA2, PARP12, FHIT, RPTOR, GSTM1, GSTM5, MAGI2, BCAN, DDB2, KDM4C, AGPAT3, CAMTA1, CCR6, CUX1, DNAH17, ELMO1, FNDC3B, GNRHR, HDAC4, IRS2, LIF, SMAD3, SOD3, TALDO1, TRIM27, GAA, PAX8, RNF39, HLA-C, HLA-DRB6), are testis enriched (NFATC1, NMNAT3, PIAS2, SRPK2, WDR36, WWP2), or show methylation differences between infertile cases and controls (PTPRN2, RPH3AL). CONCLUSION(S): We found a statistically significant correlation between peripheral blood DNA methylation and male infertility, raising the hope that epigenome-based blood markers can be used for screening male infertility risk. The study also identified new candidates for spermatogenesis and fertility.


Assuntos
Azoospermia/diagnóstico , Metilação de DNA , Fertilidade/genética , Perfilação da Expressão Gênica/métodos , Análise de Sequência com Séries de Oligonucleotídeos , Oligospermia/diagnóstico , Azoospermia/sangue , Azoospermia/genética , Azoospermia/fisiopatologia , Estudos de Casos e Controles , Ilhas de CpG , Marcadores Genéticos , Predisposição Genética para Doença , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Masculino , Oligospermia/sangue , Oligospermia/genética , Oligospermia/fisiopatologia , Fenótipo , Valor Preditivo dos Testes
8.
Andrologia ; 51(7): e13290, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30995699

RESUMO

Chimerism, a rare human disorder, is assumed to be the result of an amalgamation of two separate zygotes in a single embryo. Studies have shown that the phenotypic spectrum of chimerism is variable and there is no definite genotype-phenotype correlation in patients with chimerism, therefore a majority of cases might remain undiagnosed. This study aims to investigate the possible mechanism of the chimerism in a 46,XX/46,XY infertile and phenotypically normal male, with 46,XX blood karyotype and normal spermatogenesis. We have used Interphase-FISH analysis to study the CEPX and CEPY regions on buccal and urine samples as well as molecular analysis of polymorphic short tandem repeats (STR) markers from 34 loci in order to discover the origin of 46,XX/46,XY. Analysis of X-linked and autosomal STR markers on blood, buccal tissue, urine, fibroblast and testis biopsy samples of the proband along with the blood sample of the patient's parents and siblings, showed divergent karyotypes in different tissues and tetragametic chimerism was diagnosed.


Assuntos
Cariótipo Anormal , Quimerismo , Oligospermia/genética , Adulto , Humanos , Cariotipagem , Masculino , Oligospermia/diagnóstico
9.
Andrologia ; 51(6): e13271, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30891813

RESUMO

To evaluate whether hCG/hMG therapy has beneficial effects on idiopathic oligozoospermia in Chinese infertility population. The patients were randomly divided into the treatment group receiving hCG/hMG for 3 months and the placebo group receiving placebo for 3 months. Semen and biochemical analysis was performed, and DNA fragmentation as well as spermatid concentration was evaluated. Administration of hCG/hMG for 3 months could significantly improve sperm concentration, rate of forward motile spermatozoa, total motile sperm count, the percentage of sperm with normal morphology and the rate of spontaneous pregnancy in medium- and higher-level inhibin B group respectively. Moreover, in medium- and higher-level inhibin B group, sperm DNA fragmentation index and spermatid concentration were significantly declined respectively at the end of treatment. However, there were no significant differences in lower-level inhibin B group before and after treatment in term of seminal parameters, DNA fragmentation and spermatid concentration. HCG/hMG therapy for 3 months has a beneficial effect on a part of male with idiopathic oligozoospermia, and the efficacy of hCG/hMG therapy is associated with the inhibin B level.


Assuntos
Gonadotropina Coriônica/administração & dosagem , Menotropinas/administração & dosagem , Oligospermia/tratamento farmacológico , Adulto , Método Duplo-Cego , Combinação de Medicamentos , Feminino , Humanos , Inibinas/sangue , Masculino , Pessoa de Meia-Idade , Oligospermia/sangue , Oligospermia/diagnóstico , Placebos/administração & dosagem , Gravidez , Taxa de Gravidez , Contagem de Espermatozoides , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Resultado do Tratamento , Adulto Jovem
10.
Horm Mol Biol Clin Investig ; 39(2)2019 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-30870141

RESUMO

Objective To illustrate the importance of treatment duration with intramuscular testosterone undecanoate (Nebido®) for the final spermatogenesis recovery after treatment cessation. Also, to show a subsequent poor efficacy of the selective estrogen receptor modulator (SERM) clomiphene citrate (CC) in treating steroid-induced azoospermia following Nebido® cessation and describe that initial oligozoospermia, existing before starting Nebido®, largely contributes to that treatment outcome. Methodology Setting: Department of Human Reproduction and Department of Endocrinology, Clinical Hospital Center Rijeka, Rijeka, and Department of Endocrinology, Clinical Hospital Center Sestre milosrdnice, Zagreb, Croatia. PATIENT: A male patient having been diagnosed with primary hypogonadotropic hypogonadism, oligozoospermia and low testosterone (T) level, was treated with intramuscular testosterone undecanoate (TU) depot 1 g (Nebido®) to prevent further progression of testosterone deficiency symptoms (low mood, energy and concentration, fatigue, muscle weakness). INTERVENTIONS: Stopping Nebido® and treatment with CC 50 mg per day 5 days per week for 3-6 month to recover spermatogenesis. MAIN OUTCOME MEASURES: T levels and semen analyses. Results Semen analyses did not return to values before taking Nebido® 1 year after cessation nor after 3 months of treatment with CC. Values of T, follicle stimulating hormone (FSH) and luteinizing hormone (LH) dropped even more than before starting Nebido®, after 1 year of cessation. Conclusions Here we describe a case of initially idiopathic gonadal failure with subsequent secondary gonadal failure and infertility resulting from testosterone replacement therapy (TRT) treatment, and poor spermatogenesis recovery outcome of CC used post Nebido® cessation.


Assuntos
Clomifeno/efeitos adversos , Oligospermia/tratamento farmacológico , Oligospermia/etiologia , Testosterona/análogos & derivados , Biomarcadores , Clomifeno/administração & dosagem , Humanos , Injeções Intramusculares , Masculino , Oligospermia/diagnóstico , Contagem de Espermatozoides , Testosterona/administração & dosagem , Testosterona/efeitos adversos , Resultado do Tratamento , Adulto Jovem
11.
Eur Urol Focus ; 5(6): 1143-1145, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29729871

RESUMO

Many common sports and sports-related behaviors and practices represent potential sources of male infertility. Clinicians should be aware of these associations in the evaluation of idiopathic infertility in men.


Assuntos
Infertilidade Masculina/fisiopatologia , Espermatogênese/fisiologia , Esportes/fisiologia , Azoospermia/diagnóstico , Azoospermia/etiologia , Ciclismo/fisiologia , Humanos , Infertilidade Masculina/epidemiologia , Masculino , Metanálise como Assunto , Oligospermia/diagnóstico , Oligospermia/etiologia , Ensaios Clínicos Controlados Aleatórios como Assunto
12.
Rev Int Androl ; 16(4): 131-136, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30286866

RESUMO

OBJECTIVE: The aim of this study was to compare fertilisation, pregnancy rates and perinatal outcomes in patients undergoing intracytoplasmic sperm injection (ICSI) due to oligozoospermia. METHODS: A total of 166 patients with oligozoospermia who underwent an ICSI procedure were included in the study. The subjects were divided into two groups according to the sperm retrieval technique used: group 1, ejaculated semen (n=111); group 2, surgical sperm retrieval (n=55). RESULTS: Although the clinical pregnancy rate was lower in group 2, the difference was not statistically significant (36.4% vs. 42.3%, p=0.460). The difference between fertilisation and take-home baby rates of the groups were not significantly different, either (p=0.486, p=0.419, consecutively). CONCLUSION: Two different sperm retrieval techniques used for ICSI had no statistically significant difference on intracytoplasmic sperm injection outcomes in oligozoospermic patients.


Assuntos
Oligospermia/diagnóstico , Taxa de Gravidez , Injeções de Esperma Intracitoplásmicas/métodos , Recuperação Espermática , Adulto , Biópsia/métodos , Ejaculação , Feminino , Humanos , Masculino , Gravidez , Resultado da Gravidez , Estudos Retrospectivos , Sêmen/metabolismo , Espermatozoides/metabolismo , Adulto Jovem
13.
Andrology ; 6(4): 513-524, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-30134082

RESUMO

BACKGROUND: Oligo-astheno-teratozoospermia is frequently reported in men from infertile couples. Its etiology remains, in the majority of cases, unknown with a variety of factors to contribute to its pathogenesis. The aim of this European Academy of Andrology guideline was to provide an overview of these factors and to discuss available management options. MATERIALS AND METHODS: PubMed was searched for papers in English for articles with search terms: male infertility and oligo-astheno-teratozoospermia. For evidence-based recommendations, the GRADE system was applied. Issues related to urogenital infections/inflammations have not been included in this document as they will be covered by separate guidelines. RESULTS: For men with oligo-astheno-teratozoospermia, the European Academy of Andrology recommends: A general physical examination to assess signs of hypogonadism. A scrotal physical examination to assess (i) the testes and epididymes for volume and consistency, (ii) deferent ducts for total or partial absence, and (iii) occurrence of varicocoele. Performing two semen analyses, according to World Health Organization guidelines to define an oligo-astheno-teratozoospermia. An endocrine evaluation. A scrotal ultrasound as part of routine investigation. Karyotype analysis and assessment of Yq microdeletions in infertile men with a sperm concentration ≤5 × 106 /mL. Cystic fibrosis transmembrane conductance regulator gene evaluation in case of suspicion for incomplete congenital obstruction of the genital tract. Against quitting physical activity to improve the chance of achieving pregnancy. Against androgen replacement therapy to improve the chance of achieving pregnancy. Assisted reproduction techniques to improve the chance of achieving pregnancy, in case other treatment options are not available or not efficient. Androgen replacement therapy in patients with biochemical/clinical signs of hypogonadism, after completion of the fertility treatment. CONCLUSION: These guidelines can be applied in clinical work and indicate future research needs.


Assuntos
Oligospermia/diagnóstico , Oligospermia/terapia , Humanos , Masculino
14.
Zhonghua Nan Ke Xue ; 24(1): 27-32, 2018.
Artigo em Chinês | MEDLINE | ID: mdl-30157356

RESUMO

Objective: To investigate the characteristics of the semen parameters of native Tibetans and immigrated Tibetan Hans in the high-altitude area and analyze the influence of altitude adaptation on male fertility. METHODS: This study included 1 563 infertile male patients, including 698 native Tibetans and 865 immigrated Tibetan Hans, and 56 normal fertile men, including 33 native Tibetans and 23 Tibetan Hans. We obtained semen samples from the subjects for routine semen analysis and sperm DNA fragmentation index (DFI) examination and collected peripheral blood for determination of the reproductive hormone levels. RESULTS: In the infertile patients, the native Tibetans, as compared with the immigrated Hans, showed significantly higher incidence rates of azoospermia (5.87% vs 2.89%, P <0.05), severe oligozoospermia (3.15% vs 1.73%, P <0.05) and abnormal seminal viscosity (43.12% vs 25.89%, P<0.01), but no statistically significant differences in the percentages of normozoospermia (81.08% vs 87.39%, P >0.05), oligozoospermia (5.44% vs 3.93%, P >0.05), severe asthenozoospermia (4.44% vs 4.04%, P >0.05) or severe teratozoospermia (4.58% vs 6.59%, P >0.05). In the normal fertile men, there were no statistically significant differences between the native Tibetans and immigrated Hans in age (ï¼»32.42 ± 4.82ï¼½ vs ï¼»34.57 ± 6.01ï¼½ yr, P >0.05), sperm concentration (ï¼»143.69 ± 85.74ï¼½ vs ï¼»155.11 ± 82.56ï¼½ ×106/ml, P >0.05), straight line velocity (ï¼»25.74 ± 3.94ï¼½ vs ï¼»27.24 ± 3.46ï¼½ µm/s, P >0.05), percentage of morphologically normal sperm (ï¼»8.22 ± 4.35ï¼½ vs ï¼»7.28±2.46ï¼½ %, P >0.05), total testosterone concentration (ï¼»17.97 ± 2.98ï¼½ vs ï¼»15.72 ± 6.38ï¼½ nmol/L, P >0.05), or follicle stimulating hormone level (ï¼»5.51 ± 1.62ï¼½ vs ï¼»4.17 ± 2.08ï¼½ IU/L, P >0.05). However, the immigrated Hans, in comparison with the native Tibetans, exhibited a higher sperm motility (ï¼»79.75 ± 14.67ï¼½ vs ï¼»66.58 ± 17.21ï¼½%, P <0.05), a lower curvilinear velocity (ï¼»60.97 ± 2.71ï¼½ vs ï¼»71.14 ± 82.13ï¼½ µm/s, P <0.05) and a lower level of luteinizing hormone (ï¼»4.28 ± 1.20ï¼½ vs ï¼»5.84 ± 1.15ï¼½ IU/L, P <0.05). CONCLUSIONS: During the acclimatization to the plateau hypoxia environment, the immigrated Tibetan Hans undergo adaptive changes in sperm concentration and motility and have lower incidence rates of azoospermia and severe oligozoospermia than native Tibetan males.


Assuntos
Aclimatação/fisiologia , Altitude , Emigrantes e Imigrantes , Infertilidade Masculina/diagnóstico , Análise do Sêmen , Azoospermia/sangue , Azoospermia/diagnóstico , Azoospermia/epidemiologia , Fragmentação do DNA , Fertilidade , Humanos , Hipóxia/sangue , Hipóxia/fisiopatologia , Infertilidade Masculina/sangue , Infertilidade Masculina/epidemiologia , Hormônio Luteinizante/sangue , Masculino , Oligospermia/sangue , Oligospermia/diagnóstico , Oligospermia/epidemiologia , Sêmen , Contagem de Espermatozoides , Motilidade Espermática , Tibet , Viscosidade
15.
Andrology ; 6(6): 860-864, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30094956

RESUMO

OBJECTIVES: Awareness and acceptance of transgenderism have increased in the last two decades. There is limited literature regarding the incidence and semen characteristics of transwomen banking spermatozoa. We sought to assess the incidence of sperm cryopreservation of transgender individuals compared with the cisgender population in the last 10 years. Semen parameters were also compared between the two groups. MATERIALS AND METHODS: We performed a retrospective analysis of sperm cryopreservation performed at a single center from 2006 through 2016. Using available data on indications for banking and prior hormonal therapy status, we isolated healthy transgender and cisgender cohorts for semen parameter comparison. Linear regression was used to compare the incidence trends. Semen parameters were compared using the generalized estimating equations method. The rates of semen parameter abnormality of each group were compared using chi-square test. Semen parameter abnormalities were defined using WHO 2010 reference values. RESULTS: We analyzed 194 transgender samples and 2327 cisgender samples for a total of 84 unique transgender sperm bankers and 1398 unique cisgender sperm bankers. The number of transgender sperm bankers increased relative to cisgender sperm bankers from 2006 to 2016. Following exclusion of cisgender sperm bankers with health issues that might impact semen quality and transgender sperm bankers with known prior hormonal therapy, we compared the semen parameters of 141 healthy cisgender sperm bankers and 78 healthy transgender sperm bankers. The transgender sperm bankers demonstrated lower sperm concentration, total motile sperm count, and post-thaw sperm parameters. The transgender sperm bankers also demonstrated a higher incidence of oligozoospermia. CONCLUSIONS: This is the largest report to date on the incidence of transgender sperm cryopreservation and comparison of semen characteristics with cisgender sperm bankers. The data reveal an increased incidence of transgender sperm banking as well as poorer semen parameters of transgender individuals compared with cisgender controls.


Assuntos
Criopreservação/tendências , Conhecimentos, Atitudes e Prática em Saúde , Análise do Sêmen/tendências , Preservação do Sêmen/tendências , Pessoas Transgênero/psicologia , Transexualidade/psicologia , Adolescente , Adulto , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Oligospermia/diagnóstico , Oligospermia/epidemiologia , Estudos Retrospectivos , Contagem de Espermatozoides , Motilidade Espermática , Fatores de Tempo , Transexualidade/epidemiologia , Adulto Jovem
16.
Fertil Steril ; 110(3): 419-428.e1, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29961538

RESUMO

OBJECTIVE: To evaluate the epigenetic consequence on the methylome and subsequent transcriptome in euploid blastocysts of male-factor (MF) infertility patients. DESIGN: Methylome and transcriptome analysis on individual oligoasthenoteratozoospermia (OAT [MF]) blastocysts. SETTING: Infertility clinic. PATIENT(S): Clinical data from 128 couples presenting with OAT (MF) and 118 maternal age-matched control (no MF) subjects undergoing infertility treatment from 2010 to 2014, along with 72 surplus cryopreserved blastocysts donated from 33 couples with their informed consents. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Methyl Maxi-Seq (Zymo Research) was used to determine genome-wide DNA methylation, and small cell number RNA-Seq was used to examine the global transcriptome. Validation experiments were performed with the use of pyrosequencing or quantitative real-time polymerase chain reaction. Statistical analysis used Student t test, analysis of variance in R, Fisher exact test, and pairwise fixed reallocation randomization test where appropriate, with significance at P<.05. RESULT(S): Clinical pregnancy rates were similar between OAT (MF) patients and control (no MF) subjects after euploid embryo transfer. However, the miscarriage rate for OAT (MF) patients was significantly higher (14.7% vs. 2.2%; P<.05). Methylome and transcriptome analyses of individual blastocysts revealed significant alterations in 1,111 CpG sites and 469 transcripts, respectively (P<.05). Pathway analysis elucidated genes involved in "regulation of cellular metabolic process" as universally affected. Validation of the genome-wide approaches was performed for SBF1 and SLC6A9 (P<.05). CONCLUSION(S): Methylation and transcription aberrations in individual OAT (MF) blastocysts illustrate an epigenetic consequence of MF infertility on embryogenesis, significantly altering key developmental genes and affecting embryonic competence. This epigenetic dysregulation provides an explanation for the reduced reproductive potential in OAT (MF) patients despite euploid blastocyst transfers.


Assuntos
Transferência Embrionária/métodos , Epigênese Genética/genética , Fertilização In Vitro/métodos , Infertilidade Masculina/genética , Oligospermia/genética , Reprodução/genética , Adulto , Transferência Embrionária/tendências , Feminino , Fertilização In Vitro/tendências , Humanos , Masculino , Oligospermia/diagnóstico , Oligospermia/terapia , Gravidez , Taxa de Gravidez/tendências
17.
Andrology ; 6(4)July 2018.
Artigo em Inglês | BIGG - guias GRADE | ID: biblio-1015447

RESUMO

Oligo­astheno­teratozoospermia is frequently reported in men from infertile couples. Its etiology remains, in the majority of cases, unknown with a variety of factors to contribute to its pathogenesis. The aim of this European Academy of Andrology guideline was to provide an overview of these factors and to discuss available management options.


Assuntos
Humanos , Masculino , Oligospermia/diagnóstico , Oligospermia/terapia , Andrologia/métodos , Teratozoospermia/tratamento farmacológico
18.
Asian J Androl ; 20(5): 454-458, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29798938

RESUMO

In the present study, we evaluated the impact of sperm origins and concentration on the clinical outcomes of intracytoplasmic sperm injection (ICSI) cycles. A total of 1201 ICSI cycles were retrospectively analyzed for male azoospermia or oligozoospermia between January 2015 and December 2015 in the Peking University Third Hospital. Patients were divided into three groups (Group 1 vs Group 2/3; surgically extracted sperm vs ejaculated sperms): Group 1 included 343 ICSI cycles and Group 2 analyzed 388 cycles on semen with sperm concentration <5 × 106 ml-1 (severe oligozoospermia group). Group 3 included 470 cycles with sperm concentration between 5 × 106 ml-1 and 15 × 106 ml-1 (mild oligozoospermia group). Fertilization rates, clinical pregnancy rates, and live birth rates were analyzed and compared among groups of different semen origins and concentrations on the oocyte retrieval day. Group 2 showed a lower fertilization rate than Group 3 (62.9% ± 21.6% vs 66.8% ± 22.1%,P< 0.05). There were no statistically significant differences in clinical pregnancy rate per transfer (51.3%, 46.7%, and 50.0%, respectively), live birth rate per transfer (44.4%, 40.9%, and 41.4%, respectively), accumulative live birth rate (58.3%, 51.0%, and 52.1%, respectively), twin birth rate (18.4%, 10.6%, and 12.6%, respectively), and birth defects rate (0, 0.3%, and 0.2%, respectively) among three groups. The results of this study indicated that sperm origins and concentration do not impact the clinical outcomes in ICSI cycles.


Assuntos
Azoospermia/diagnóstico , Coeficiente de Natalidade , Oligospermia/diagnóstico , Taxa de Gravidez , Adulto , Feminino , Humanos , Nascimento Vivo , Masculino , Gravidez , Estudos Retrospectivos , Análise do Sêmen , Injeções de Esperma Intracitoplásmicas/métodos
19.
Chin J Integr Med ; 24(7): 483-486, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29700764

RESUMO

Oligo-astheno-teratozoospermia (OAT) which perplexes doctors and patients due to its complicated etiology, atypical symptoms and poor clinical efficacy, is a general term for the three pathological states of abnormal semen. OAT is the main factor of male infertility. It is also a hot and difficult point in present studies. Empiric drug is the most popular treatment of this disease in the modern medicine. Chinese medicine (CM) is one of the main methods for the treatment of this disease, and it has certain clinical effect. The author believes that the use of modern medical technology to make the diagnosis as clear as possible is the key to treat OAT. The combination of syndrome and disease differentiation is the main mode in the treatment of OAT. Microscopic syndrome differentiation and macroscopic evidence embodies the basic principle of "Si Wai Chuai Nei" and broadens the perspective of CM syndrome differentiation. Classification and treatment are basic methods for the treatment of OAT. The treatment should not be limited to the Shen (Kidney), instead it should focus on the whole body condition. At different stages, the treatment should also pay attention to strengthening the Pi (Spleen), nourishing the Gan (Liver) and promoting blood circulation. Complementing Chinese and Western medicine, and highlighting the characteristics and advantages of CM treatment, have a great guiding value for the treatment of OAT.


Assuntos
Astenozoospermia/terapia , Medicina Integrativa/métodos , Medicina Tradicional Chinesa/métodos , Oligospermia/terapia , Astenozoospermia/diagnóstico , Terapia Combinada , Diagnóstico Diferencial , Humanos , Masculino , Oligospermia/diagnóstico , Análise do Sêmen , Ocidente
20.
Medicine (Baltimore) ; 97(15): e0452, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29642220

RESUMO

RATIONALE: Infertile male carrying balanced translocations can be broadly divided into two types: pregestational and gestational infertility. Chromosome and breakpoints involved translocation should be considered in genetic counselling for these patients. To date, > 100 cases have been described with carrying balanced translocations involving chromosome 10 in fertile male. PATIENT CONCERNS: We report 11 cases translocation carriers involving chromosome 10, and review 99 carriers of chromosome 10 translocation from reported literature. DIAGNOSES: Eleven cases of chromosomal translocation were diagnosed by cytogenetic analysis. Three of these men had azoospermia or oligozoospermia, while eight had normal semen. Of these latter cases, their partners were able to conceive, but had a tendency to miscarry or have a stillbirth. INTERVENTIONS: Chromosome breakpoints should be considered in genetic counseling. Preimplantation genetic diagnosis should be performed to decrease the high risk of miscarriage and to minimize the genetic risks to offspring for patients with gestational infertility. OUTCOMES: The most common translocations and breakpoints were at t(4;10) and 10q24, observed in 12 and 10 patients respectively. Breakpoints at 10p15.1, 10p12, 10q10, 10q22.1, 10q24.2, and 10q26.3 were linked to pregestational infertility; breakpoints at 10p12.1, 10q11, 10q21.2, and 10q23.3 were associated with gestational infertility; the other breakpoints were connected with both forms of infertility. LESSONS: Breakpoints at 10p12 and 10q26.3 were associated with pregestational infertility. Other breakpoints at chromosome 10 were correlated with gestational infertility. These breakpoints should be considered when counseling men with chromosome 10 translocations should be informed of their options.


Assuntos
Cromossomos Humanos Par 10/genética , Triagem de Portadores Genéticos , Infertilidade Masculina/genética , Translocação Genética/genética , Adulto , Azoospermia/diagnóstico , Azoospermia/genética , Pontos de Quebra do Cromossomo , Feminino , Aconselhamento Genético , Humanos , Cariotipagem , Masculino , Oligospermia/diagnóstico , Oligospermia/genética , Gravidez , Diagnóstico Pré-Implantação
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