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1.
J Pediatr Urol ; 15(4): 342.e1-342.e7, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31036478

RESUMO

BACKGROUND: Little is known about sperm health in male patients with familial Mediterranean fever (FMF). In this study, the authors aimed to search the frequency of sperm abnormalities of adolescent boys with FMF and also to investigate whether disease activity or colchicine treatment have negative effects on sperm parameters. METHOD: The male adolescents older than 14 years with a diagnosis of FMF were investigated retrospectively. Tel Hashomer and pediatric FMF clinical criteria were used for diagnosis of FMF. Patients who had semen analysis were included in the study. RESULT: Mean age at the diagnosis was 11.13 ± 3.82 years, and mean age at the study was 14.50 ± 0.70 years. The mean sperm concentration was found as 66.26 ± 41.02 million/ml (N > 15 million/ml), the mean total sperm count 113.42 ± 132.39 million (N > 39 million), and the mean sperm motility 51.78 ± 23.70% (N > 40%). Only 8 of 19 (42.1%) patients had normal sperm parameters. Sperm concentration was reduced in two cases, total sperm count was reduced in four patients, and motility was reduced in nine cases. The presence of FMF attacks under treatment was found to be a risk factor for decreased motility in the study group by multivariate regression analysis (odds ratio 0.076, [95% confidence interval 0.005-0.648], P = 0.031). Erythrocyte sedimentation rate at the time of diagnosis was high in patients with low sperm counts compared with those with normal sperm counts (56.00 ± 8.51 vs 24.35 ± 6.32, P: 0.03). Mean colchicine dose at the time of sperm analysis was higher in patients with low sperm motility than that with normal sperm motility (1.72 ± 0.18 vs 1.25 ± 0.08, P: 0.02). CONCLUSION: Sperm abnormalities of male patients with FMF is not infrequent, and it is linked to both inflammation due to uncontrolled disease and colchicine therapy.


Assuntos
Colchicina/efeitos adversos , Febre Familiar do Mediterrâneo/complicações , Febre Familiar do Mediterrâneo/diagnóstico , Infertilidade Masculina/prevenção & controle , Oligospermia/etiologia , Adolescente , Distribuição de Qui-Quadrado , Criança , Estudos de Coortes , Colchicina/uso terapêutico , Febre Familiar do Mediterrâneo/tratamento farmacológico , Humanos , Incidência , Masculino , Oligospermia/epidemiologia , Oligospermia/fisiopatologia , Modelos de Riscos Proporcionais , Estudos Retrospectivos , Análise do Sêmen/métodos , Índice de Gravidade de Doença , Motilidade Espermática , Turquia
2.
Front Biosci (Landmark Ed) ; 24: 1401-1425, 2019 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-31136987

RESUMO

Currently, there are few male contraceptive methods that are purely based on prevention of the entry of the sperm into the female reproductive tract. An alternative approach for designing reversible male contraceptive is achieved by transient testicular heating (TTH). This treatment, through massive germ cell apoptosis, causes reversible oligospermia or azoospermia. Here, we describe as how TTH causes DNA damage, oxidative stress, apoptosis, autophagy, sperm protein expression, and alters the biochemical components of seminal plasma. Further understanding of TTH will help design safe and reversible male contraception.


Assuntos
Calefação/métodos , Temperatura Alta , Oligospermia/fisiopatologia , Sêmen/fisiologia , Espermatozoides/fisiologia , Testículo/fisiopatologia , Anticoncepção/métodos , Dano ao DNA , Humanos , Masculino , Oligospermia/metabolismo , Sêmen/metabolismo , Espermatozoides/metabolismo , Testículo/metabolismo , Testículo/patologia
3.
Fertil Steril ; 112(1): 61-72.e1, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31103287

RESUMO

OBJECTIVE: To study peripheral blood DNA differential methylation in oligozoospermic infertile men in comparison with normozoospermic fertile controls. DESIGN: Case-control study. SETTING: Reproductive biology laboratory. PATIENTS(S): Azoospermic and oligozoospermic infertile patients (n = 6) and normozoospermic fertile controls (n = 6) in the discovery phase, and oligo/asthenozoospermic infertile men (n = 11) and normozoospermic fertile controls (n = 10) in the validation phase. INTERVENTION(S): Blood samples drawn from all participants, DNA isolation and methylation analysis. MAIN OUTCOME MEASURE(S): DNA methylation values analyzed using genomewide methylation 450K BeadChip array, followed by deep sequencing of selected regions for methylation analysis in the neighborhood regions of differentially methylated CpGs. RESULT(S): We found 329 differentially methylated CpG spots, out of which 245 referred to the genes, representing 170 genes. Deep-sequencing analysis confirmed the methylation pattern suggested by 450K array. A thorough literature search suggested that 38 genes play roles in spermatogenesis (PDHA2, PARP12, FHIT, RPTOR, GSTM1, GSTM5, MAGI2, BCAN, DDB2, KDM4C, AGPAT3, CAMTA1, CCR6, CUX1, DNAH17, ELMO1, FNDC3B, GNRHR, HDAC4, IRS2, LIF, SMAD3, SOD3, TALDO1, TRIM27, GAA, PAX8, RNF39, HLA-C, HLA-DRB6), are testis enriched (NFATC1, NMNAT3, PIAS2, SRPK2, WDR36, WWP2), or show methylation differences between infertile cases and controls (PTPRN2, RPH3AL). CONCLUSION(S): We found a statistically significant correlation between peripheral blood DNA methylation and male infertility, raising the hope that epigenome-based blood markers can be used for screening male infertility risk. The study also identified new candidates for spermatogenesis and fertility.


Assuntos
Azoospermia/diagnóstico , Metilação de DNA , Fertilidade/genética , Perfilação da Expressão Gênica/métodos , Análise de Sequência com Séries de Oligonucleotídeos , Oligospermia/diagnóstico , Azoospermia/sangue , Azoospermia/genética , Azoospermia/fisiopatologia , Estudos de Casos e Controles , Ilhas de CpG , Marcadores Genéticos , Predisposição Genética para Doença , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Masculino , Oligospermia/sangue , Oligospermia/genética , Oligospermia/fisiopatologia , Fenótipo , Valor Preditivo dos Testes
4.
Ups J Med Sci ; 124(2): 111-118, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30957617

RESUMO

Background: Extracellular vesicles (EVs), released from the plasma membrane or intracellular compartments, have a specific composition related to their parent cells, but they can, additionally, be modified by the extracellular environment. Although glycans are known to contribute to EV composition and may have biomedical importance as biomarkers and recognition signals, they have not been extensively investigated. In this study, seminal prostasomes, i.e. EVs from seminal plasma (SP) of normo- and oligozoospermic men, were analyzed in order to detect possible changes in their surface glycans under altered physiological conditions. Methods: Prostasomes were isolated from pooled SP by differential centrifugation and gel filtration, followed by glycobiochemical characterization using lectin/immune-transmission microscopy and ion-exchange chromatography. Results: Within the frame of overall similarity in protein composition, surface glycans specifically contributed to the differences between the examined groups of prostasomes in terms of presentation of sialylated and mannosylated moieties. These changes did not affect their anti-oxidative capacity, but implied a possible influence on the accessibility of galectin-3 to its ligands on the prostasomal surface. Conclusions: Subtle differences in the presentation of surface molecules may be helpful for differentiation among vesicles sharing the same physical properties. In addition, this may point to some unexpected regulatory mechanisms of interaction of distinct populations of vesicles with their binding partners.


Assuntos
Oligospermia/metabolismo , Oligospermia/fisiopatologia , Polissacarídeos/química , Próstata/metabolismo , Sêmen/metabolismo , Espermatozoides/metabolismo , Antioxidantes/química , Biomarcadores/metabolismo , Centrifugação , Cromatografia em Gel , Cromatografia por Troca Iônica , Galectina 3/química , Glicosilação , Humanos , Ligantes , Masculino , Microscopia Eletrônica de Transmissão , Ácido N-Acetilneuramínico/química , Ligação Proteica , Espécies Reativas de Oxigênio/metabolismo
5.
Acupunct Med ; 37(1): 25-32, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30942613

RESUMO

OBJECTIVE: To evaluate the effect of transcutaneous electrical acupuncture point stimulation (TEAS) on sperm parameters and the underlying molecular mechanisms. METHODS: A total of 121 patients diagnosed with oligozoospermia, asthenozoospermia or oligoasthenozoospermia were randomised into four groups (three treatment groups, one control): the TEAS groups were treated with 2 Hz (n=31), 100 Hz (n=31), or mock stimulation (n=29) at acupuncture points BL23, ST36, CV1 and CV4 for 2 months. The control group (n=30) was provided with lifestyle advice only. RESULTS: The changes in total sperm count and motility in the 2 Hz TEAS group were significantly greater than those in the mock group and the control group. The change in neutral α-glucosidase (NAG) and zinc levels in the 2 Hz group were significantly greater than those in the mock group and control group, and the changes in fructose levels of the 2 Hz group were significantly greater than those in the control group. Significant increases in calcium and integrin-binding protein 1 (CIB1) and reduction of cyclin-dependent kinase 1 b (CDK1) were also found after 2 Hz TEAS treatment. CONCLUSIONS: The present findings suggest that 2 Hz TEAS can improve sperm count and motility in patients with abnormal semen parameters, and is associated with increases in seminal plasma zinc, NAG and fructose. The upregulation of CIB1 and downregulation of CDK1 by TEAS may be associated with its positive effects on sperm motility and count. TRIAL REGISTRATION: http://www.chictr.org ; registration no. ChiCTR-TRC-11001775.


Assuntos
Astenozoospermia/terapia , Eletroacupuntura , Oligospermia/terapia , Sêmen/metabolismo , Pontos de Acupuntura , Adulto , Astenozoospermia/metabolismo , Astenozoospermia/fisiopatologia , Humanos , Masculino , Pessoa de Meia-Idade , Oligospermia/metabolismo , Oligospermia/fisiopatologia , Sêmen/citologia , Contagem de Espermatozoides , Motilidade Espermática , Espermatozoides/citologia , Resultado do Tratamento
6.
Cryobiology ; 86: 65-70, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30537469

RESUMO

Application of an appropriate freezing carrier is crucial for improving post-thaw recovery of oligozoospermic samples. In this study, our purpose is developing a user-friendly, easy handling and close micro-quantity (MQ) straw along with different freezing media, for cryopreservation of oligozoospermic samples. Twenty oligozoospermic semen samples were collected and mixed with glycerol egg yolk citrate (GEYC) or Spermfreeze® (SPF) medium. The mixture was loaded into MQ straws, sealed and stored in liquid nitrogen (LN) vapor. After freezing, the straws were transferred into cryotube and plunged into LN. Post-thawed sperm parameters including motion characteristics, viability, membrane and DNA integrity were evaluated one and three months after cryopreservation. The post-thawed sperm parameters were significantly reduced in GEYC and SPF medium compared to fresh samples. No statistically significant differences were seen in sperm characteristics between the two storage times (i.e. month 1 vs. month 3). Furthermore, GEYC medium yielded higher motility, viability and membrane integrity compared to SPF at both storage time-points. Sperm DNA integrity was also improved in GEYC group compared to SPF after 1 month of storage. The findings of our study showed that application of MQ straw along with GEYC, as the cryoprotectant, was beneficial for cryopreservation of low count semen samples.


Assuntos
Criopreservação/métodos , Crioprotetores/farmacologia , Oligospermia/fisiopatologia , Preservação do Sêmen/métodos , Motilidade Espermática/efeitos dos fármacos , Animais , Ácido Cítrico/farmacologia , Criopreservação/instrumentação , Congelamento , Glicerol/farmacologia , Masculino , Sêmen/efeitos dos fármacos , Preservação do Sêmen/instrumentação , Espermatozoides/efeitos dos fármacos
8.
Int Braz J Urol ; 44(5): 972-980, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30130018

RESUMO

PURPOSE: To evaluate the impact of overactive bladder disorder on patients diagnosed with retrograde ejaculation. MATERIALS AND METHODS: Retrospective analysis of prospective collected database made. Questionnaires conducted in urology polyclinics in five different centers. MAIN OUTCOME MEASURE(S): International Index of Erectile Function - 5 (IIEF - 5), Overactive Bladder 8 - Question Awareness Tool (OAB - V8), urodynamics, semen analysis. The participants of the study were n = 120 patients. There was retrograde ejaculation (RE) in only n = 47 patients (non / minimal symptomatic patients), n = 73 patients had RE and overactive (OAB) complaints (symptomatic patients) and received anticholinergic treatment (trospium), n = 37 control group patients who only had OAB and received an anticholinergic. RESULTS: While no difference was observed in overactive bladder examination and urodynamic values between the non / minimal symptomatic group and the symptomatic group (p > 0.05), sperm was detected and identified as fructose positive in post - ejaculation urine in the symptomatic group. Thus, it was possible to demonstrate the differences between symptomatic patients and non - symptomatic patients. Consequently, following three - month daily treatment with trospium 30 mg 2 x 1 in the control group and the symptomatic group, it was observed that an evident increase was observed in the sperm count and ejaculate volume in the symptomatic group and that no change was observed in the control group (p < 0.05). CONCLUSION: This clinical study is the first of its kind in terms of revealing the coexistence of RE with OAB upon performing urodynamics and showing that treatment is possible in selected patients.


Assuntos
Antagonistas Colinérgicos/uso terapêutico , Ejaculação/fisiologia , Oligospermia/complicações , Bexiga Urinária Hiperativa/complicações , Adulto , Estudos de Casos e Controles , Humanos , Masculino , Oligospermia/tratamento farmacológico , Oligospermia/fisiopatologia , Estudos Retrospectivos , Contagem de Espermatozoides , Bexiga Urinária Hiperativa/tratamento farmacológico , Bexiga Urinária Hiperativa/fisiopatologia
9.
Isr Med Assoc J ; 20(4): 250-253, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29629734

RESUMO

BACKGROUND: Male infertility is solely responsible for approximately 20% of all infertility in couples. Various factors have been proposed as having a negative effect on sperm quality; however, the reasons for the global decline in sperm parameters during the last few decades are still controversial. OBJECTIVES: To investigate the fluctuations of semen parameters (sperm concentration, motility, and morphology) in three sperm quality groups and to examine the trends of those parameters in the same men over time. RESULTS: Our data showed deterioration in all semen parameters assessed in the group of men originally considered as having normal semen values according to the 2010 criteria of the World Health Organization. In contrast, we found significant improvement over time in all semen parameters in the group of men with severe oligo-terato-asthenozoospermia. CONCLUSIONS: Our results suggest that, although there were changes in sperm quality over time in the groups assessed, the clinical significance is negligible and does not necessarily justify a change in the therapeutic approach to infertility or sperm cryopreservation.


Assuntos
Infertilidade Masculina/fisiopatologia , Sêmen/fisiologia , Contagem de Espermatozoides/métodos , Motilidade Espermática/fisiologia , Espermatozoides/fisiologia , Astenozoospermia/fisiopatologia , Estudos de Coortes , Seguimentos , Humanos , Masculino , Oligospermia/fisiopatologia , Estudos Retrospectivos , Índice de Gravidade de Doença , Teratozoospermia/fisiopatologia , Fatores de Tempo
10.
Andrologia ; 50(2)2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28762516

RESUMO

To study the outcome of FISH sperm examination in cases with sperm pathology and outline the potential correlation with certain chromosomal defects. A retrospective study of prospectively collected data was performed in IAKENTRO, Infertility Treatment Center. Rates of abnormal FISH semen examination were compared between male infertility patients and fertile controls. Detection of abnormal FISH semen examination as well as each chromosomal abnormality detected was correlated with each sperm deficiency (asthenozoospermia, oligozoospermia and teratozoospermia) in a univariate regression model. There were 72 male partners included, of which 52 male infertility patients and 20 controls. The rate of abnormal sperm FISH examination was significantly higher in patients' group (55.8% vs. 15.0% for controls, p = .002). Asthenozoospermia, oligozoospermia and teratozoospermia were significantly correlated with detection of abnormal FISH examination (p = .004, p = .01 and p < .001 respectively). Teratospermia was significantly correlated with increased aneuploidy rate for chromosome 17 (p = .005), chromosome X (p = .05) and Y (p = .03). FISH examination reveals pathology in a significant proportion of patients with sperm defects and should be recommended to achieve early detection of chromosomal defects that may postpone favourable reproductive outcome.


Assuntos
Astenozoospermia/fisiopatologia , Oligospermia/fisiopatologia , Análise do Sêmen/métodos , Espermatozoides/patologia , Teratozoospermia/fisiopatologia , Adulto , Aneuploidia , Astenozoospermia/genética , Estudos de Casos e Controles , Cromossomos Humanos Par 17/genética , Cromossomos Humanos X/genética , Cromossomos Humanos Y/genética , Humanos , Hibridização in Situ Fluorescente , Masculino , Oligospermia/genética , Estudos Prospectivos , Estudos Retrospectivos , Teratozoospermia/genética
11.
J Int Med Res ; 46(1): 107-114, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28730893

RESUMO

Objective * Chong Xie, Xiangfeng Chen, and Yulin Liu contributed equally to this work. Genetic defects are identified in nearly 20% of infertile males. Determining the frequency and types of major genetic abnormalities in severe male infertility helps inform appropriate genetic counseling before assisted reproductive techniques. Methods Cytogenetic results of 912 patients with non-obstructive azoospermia (NOA) and severe oligozoospermia (SOS) in Eastern China were reviewed in this multicenter study from January 2011 to December 2015. Controls were 215 normozoospermic men with offspring. Results Among all patients, 22.6% (206/912) had genetic abnormalities, including 27.3% (146/534) of NOA patients and 15.9% (60/378) of SOS patients. Chromosomal abnormalities (all autosomal) were detected in only 1.9% (4 /215) of controls. In NOA patients, sex chromosomal abnormalities were identified in 25.8% (138/534), of which 8% (43/534) had a 47,XXY karyotype or its mosaic; higher than the SOS group prevalence (1.1%; 4/378). The incidence of Y chromosome microdeletions was lower in the SOS group (13.2%; 50/378) than in the NOA group (17.8%; 95/534). Conclusions The high prevalence of genetic abnormalities in our study indicates the importance of routine genetic testing in severe male infertility diagnosis. This may help determine the choice of assisted reproductive technique and allow specific pre-implantation genetic testing to minimize the risk of transmitting genetic defects.


Assuntos
Azoospermia/diagnóstico , Aberrações Cromossômicas/estatística & dados numéricos , Testes Genéticos/estatística & dados numéricos , Infertilidade Masculina/diagnóstico , Oligospermia/diagnóstico , Transtornos do Cromossomo Sexual no Desenvolvimento Sexual/diagnóstico , Adulto , Azoospermia/genética , Azoospermia/fisiopatologia , China , Deleção Cromossômica , Cromossomos Humanos Y/genética , Humanos , Infertilidade Masculina/genética , Infertilidade Masculina/fisiopatologia , Cariotipagem , Masculino , Pessoa de Meia-Idade , Oligospermia/genética , Oligospermia/fisiopatologia , Técnicas de Reprodução Assistida , Índice de Gravidade de Doença , Aberrações dos Cromossomos Sexuais , Transtornos do Cromossomo Sexual no Desenvolvimento Sexual/genética , Transtornos do Cromossomo Sexual no Desenvolvimento Sexual/fisiopatologia
12.
Mol Genet Metab ; 123(1): 43-49, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-29198592

RESUMO

Tangier disease is an autosomal recessive disorder caused by mutations in the ABCA1 gene and characterized by the accumulation of cholesteryl ester in various tissues and a near absence of high-density lipoprotein. The subject in this investigation was a 36-year-old Italian man with Tangier disease. He and his wife had come to the In Vitro Fertilization Unit, Pesaro Hospital (Azienda Ospedaliera Ospedali Riuniti Marche Nord) seeking help regarding fertility issues. The man was diagnosed with severe oligoasthenoteratozoospermia. Testosterone is the sex hormone necessary for spermatogenesis and cholesterol is its precursor; hence, we hypothesized that the characteristic cholesterol deficiency in Tangier disease patients could compromise their fertility. The aim of the study was to therefore to determine if there is an association between Tangier disease and male infertility. After excluding viral, infectious, genetic and anatomical causes of the subject's oligoasthenoteratozoospermia, we performed a hormonal analysis to verify our hypothesis. The patient was found to be negative for frequent bacteria and viruses. The subject showed a normal male karyotype and tested negative for Yq microdeletions and Cystic Fibrosis Transmembrane Conductance Regulator gene mutations. A complete urological examination was performed, and primary hypogonadism was also excluded. Conversely, hormonal analyses showed that the subject had a high level of follicle stimulating hormone and luteinizing hormone, low total testosterone and a significant decline in inhibin B. We believe that the abnormally low cholesterol levels typically found in subjects with Tangier disease may result in a reduced testosterone production which in turn could affect the hormonal axis responsible for spermatogenesis leading to a defective maturation of spermatozoa.


Assuntos
Colesterol/genética , Infertilidade Masculina/genética , Doença de Tangier/genética , Testosterona/biossíntese , Transportador 1 de Cassete de Ligação de ATP/genética , Adulto , Colesterol/deficiência , Ésteres do Colesterol/genética , Ésteres do Colesterol/metabolismo , Humanos , Infertilidade Masculina/complicações , Infertilidade Masculina/fisiopatologia , Masculino , Mutação , Oligospermia/complicações , Oligospermia/genética , Oligospermia/fisiopatologia , Espermatogênese/genética , Doença de Tangier/complicações , Doença de Tangier/fisiopatologia
13.
Sci Rep ; 7(1): 15778, 2017 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-29150651

RESUMO

Spermatogenesis is a multifactorial process that forms differentiated sperm cells in a complex microenvironment. This process involves the genome, epigenome, transcriptome, and proteome to ensure the stability of the spermatogonia and supporting cells. The identification of signaling pathways linked to infertility has been hampered by the inherent complexity and multifactorial aspects of spermatogenesis. Systems biology is a promising approach to unveil underlying signaling pathways and genes and identify putative biomarkers. In this study, we analyzed thirteen microarray libraries of infertile humans and mice, and different classes of male infertility were compared using differentially expressed genes and functional enrichment analysis. We found regulatory processes, immune response, glutathione transferase and muscle tissue development to be among the most common biological processes in up-regulated genes, and genes involved in spermatogenesis were down-regulated in maturation arrest (MArrest) and oligospermia cases. We also observed the overexpression of genes involved in steroid metabolism in post-meiotic and meiotic arrest. Furthermore, we found that the infertile mouse model most similar to human MArrest was the Dazap1 mutant mouse. The results of this study could help elucidate features of infertility etiology and provide the basis for diagnostic markers.


Assuntos
Infertilidade Masculina/fisiopatologia , Animais , Azoospermia/congênito , Azoospermia/genética , Azoospermia/fisiopatologia , Regulação para Baixo/genética , Perfilação da Expressão Gênica , Ontologia Genética , Humanos , Infertilidade Masculina/genética , Masculino , Camundongos , Oligospermia/genética , Oligospermia/fisiopatologia , Análise de Componente Principal , Teratozoospermia/genética , Teratozoospermia/fisiopatologia , Regulação para Cima/genética
14.
Urology ; 108: 90-95, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28712886

RESUMO

OBJECTIVE: To assess the effects of abstinence time on semen parameters in normozoospermic and oligozoospermic men using a large cohort of subfertile men. MATERIALS AND METHODS: From 2002 to 2013, we retrospectively reviewed data from 15,623 patients seen at our fertility clinic. Data on patient age and semen parameters were extracted along with abstinence time. Abstinence time was categorized into 4 groups (≤2 days; >2 and ≤5 days; >5 and ≤7 days; and >7 days). Semen samples were further categorized as normozoospermic or oligozoospermic based on concentration. Age-adjusted linear mixed effect regression models were used to test the effect of abstinence categories on semen parameters. RESULTS: Data from 11,782 encounters (10,095 patients) were used for the final analysis after excluding patients <18 years old, azoospermic samples, and those missing all semen parameters. Mean age was 32.4 (standard deviation: 6.5) and median abstinence time was 4.0 days. There were 9840 normozoospermic and 1939 oligozoospermic samples. In normozoospermic men, longer abstinence was associated with increases in ejaculate volume, concentration, total sperm count, and total motile sperm count. However, in oligozoospermic men, longer abstinence time was not associated with improvements in semen parameters except ejaculate volume. CONCLUSION: The effects of abstinence are different on semen parameters in normozoospermic and oligozoospermic patients. Longer abstinence does not improve most semen parameters in oligozoospermic samples. The World Health Organization recommendations for 2-7 days of abstinence may not be beneficial for subfertile patients when timing is a factor.


Assuntos
Oligospermia/fisiopatologia , Sêmen/fisiologia , Abstinência Sexual/estatística & dados numéricos , Motilidade Espermática/fisiologia , Adulto , Seguimentos , Humanos , Incidência , Masculino , Oligospermia/epidemiologia , Oligospermia/psicologia , Estudos Retrospectivos , Contagem de Espermatozoides , Fatores de Tempo , Utah/epidemiologia
15.
Aging Cell ; 16(5): 1035-1042, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-28613007

RESUMO

Transcriptional coactivator with PDZ-binding motif (TAZ) directly interacts with transcription factors and regulates their transcriptional activity. Extensive functional studies have shown that TAZ plays critical regulatory roles in stem cell proliferation, differentiation, and survival and also modulates the development of organs such as the lung, kidney, heart, and bone. Despite the importance of TAZ in stem cell maintenance, TAZ function has not yet been evaluated in spermatogenic stem cells of the male reproductive system. Here, we investigated the expression and functions of TAZ in mouse testis. TAZ was expressed in spermatogenic stem cells; however, its deficiency caused significant structural abnormalities, including atrophied tubules, widened interstitial space, and abnormal Leydig cell expansion, thereby resulting in lowered sperm counts and impaired fertility. Furthermore, TAZ deficiency increased the level of apoptosis and senescence in spermatogenic cells and Leydig cells upon aging. The expression of senescence-associated ß-galactosidase (SA-ßgal), secretory phenotypes, and cyclin-dependent kinase inhibitors (p16, p19, and p21) significantly increased in the absence of TAZ. TAZ downregulation in testicular cells further increased SA-ßgal and p21 expression induced by oxidative stress, whereas TAZ overexpression decreased p21 induction and prevented senescence. Mechanistic studies showed that TAZ suppressed DNA-binding activity of p53 through a direct interaction and thus attenuated p53-induced p21 gene transcription. Our results suggested that TAZ may suppress apoptosis and premature senescence in spermatogenic cells by inhibiting the p53-p21 signaling pathway, thus playing important roles in the maintenance and control of reproductive function.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/genética , Envelhecimento/genética , Inibidor de Quinase Dependente de Ciclina p21/genética , Oligospermia/genética , Espermatogônias/metabolismo , Proteína Supressora de Tumor p53/genética , Proteínas Adaptadoras de Transdução de Sinal/deficiência , Envelhecimento/metabolismo , Animais , Apoptose , Senescência Celular , Inibidor p16 de Quinase Dependente de Ciclina/genética , Inibidor p16 de Quinase Dependente de Ciclina/metabolismo , Inibidor de Quinase Dependente de Ciclina p19/genética , Inibidor de Quinase Dependente de Ciclina p19/metabolismo , Inibidor de Quinase Dependente de Ciclina p21/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Células Intersticiais do Testículo/metabolismo , Células Intersticiais do Testículo/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Oligospermia/metabolismo , Oligospermia/fisiopatologia , Transdução de Sinais , Espermatogênese/genética , Espermatogônias/patologia , Células-Tronco/metabolismo , Células-Tronco/patologia , Transativadores , Proteína Supressora de Tumor p53/metabolismo , beta-Galactosidase/genética , beta-Galactosidase/metabolismo
16.
Andrology ; 5(4): 832-837, 2017 07.
Artigo em Inglês | MEDLINE | ID: mdl-28637085

RESUMO

Herein, we report the clinical outcomes following intracytoplasmic sperm injection (ICSI) with cryopiece cryopreserved rare human spermatozoa from severe male factor infertility patients. We established a novel cryopiece system on the basis of previous studies. In this study, 126 spermatozoa from four patients with non-obstructiveazoospermia (NOA) or severe oligozoospermia were stored in cryopiece and then thawed on the day of the oocyte retrieval, 88 (83%) spermatozoa were recovered with a 47.5% (38 of 80) motile rate. Routine ICSI were performed in enrolled 30 MII oocytes from their spouse with their own spermatozoa, respectively. Twenty-two (73%) fertilization and 19 (86%) zygote cleavage were observed. Finally, a total of 11 embryos were achieved and each female patient received a transplantation of two fresh embryos. Four healthy babies were born at term. In conclusion, our novel cryopiece can be applied in assisted reproduction through ICSI with an effective motile spermatozoa recovery rate, high fertilization rate, and successful pregnancy result.


Assuntos
Azoospermia/terapia , Criopreservação/instrumentação , Fertilidade , Oligospermia/terapia , Preservação do Sêmen/instrumentação , Injeções de Esperma Intracitoplásmicas , Espermatozoides , Adulto , Azoospermia/diagnóstico , Azoospermia/fisiopatologia , Transferência Embrionária , Desenho de Equipamento , Feminino , Humanos , Nascimento Vivo , Masculino , Oligospermia/diagnóstico , Oligospermia/fisiopatologia , Gravidez , Preservação do Sêmen/métodos , Motilidade Espermática , Resultado do Tratamento
17.
Andrology ; 5(4): 704-710, 2017 07.
Artigo em Inglês | MEDLINE | ID: mdl-28440964

RESUMO

The methylenetetrahydrofolate reductase (MTHFR) gene codes a crucial enzyme which involve in folate metabolism. The effect of MTHFR gene polymorphisms on male fertility status is uncertain and controversial. We evaluated the effect of B vitamin family intake on total homocysteine content and semen parameters of men with MTHFR gene polymorphisms. MTHFR genotypes frequency and serum total homocysteine concentration were measured among 280 men with impaired spermatogenesis (asthenospermia, oligospermia, severe oligospermia and azoospermia) and 85 control participants. B vitamin family dietary intakes were assessed using a semi-quantitative food-frequency questionnaire. In addition, concentrations of vitamins B9 and B12 were evaluated in serum samples of some participants (n = 60). We observed significantly higher frequency of TC or TT genotypes in C677T polymorphism among oligospermic, severe oligospermic and azoospermic men. CC genotype of A1298C polymorphism was significantly higher only in azoospermic men. Also, we observed critical effect of vitamin B9 and B12 intake on decreasing of total homocysteine and improving of semen parameters among the men with T allele of MTHFR C677T polymorphism. Our investigation showed that sufficient consumption of vitamins B9 and B12 influences sperm parameters of men with different MTHFR polymorphisms, especially genotypes with T allele.


Assuntos
Azoospermia/tratamento farmacológico , Suplementos Nutricionais , Fertilidade/efeitos dos fármacos , Ácido Fólico/administração & dosagem , Homocisteína/sangue , Metilenotetra-Hidrofolato Redutase (NADPH2)/genética , Oligospermia/tratamento farmacológico , Polimorfismo Genético , Vitamina B 12/administração & dosagem , Adulto , Azoospermia/sangue , Azoospermia/genética , Azoospermia/fisiopatologia , Estudos de Casos e Controles , Fertilidade/genética , Ácido Fólico/metabolismo , Frequência do Gene , Heterozigoto , Homozigoto , Humanos , Irã (Geográfico) , Masculino , Metilenotetra-Hidrofolato Redutase (NADPH2)/metabolismo , Oligospermia/sangue , Oligospermia/genética , Oligospermia/fisiopatologia , Fenótipo , Índice de Gravidade de Doença , Vitamina B 12/metabolismo
18.
Andrology ; 5(4): 691-694, 2017 07.
Artigo em Inglês | MEDLINE | ID: mdl-28395120

RESUMO

Deletions on the long arm of the Y chromosome are a well-known cause of male infertility and it is generally accepted that deletions involving the AZFb region are not compatible with sperm production. Here, we report on two patients for whom basic diagnostic tests showed a deletion of the AZFb region. Unexpectedly, both patients had some residual sperm production. Subsequently, extension and additional analyses of the AZFb region disclosed an aberrant deletion pattern. Therefore, these results emphasize the need for a detailed and powerful analysis of cases where first-line Yq deletion tests reveal an AZFb deletion. Moreover, our study clearly demonstrated that only a very careful selection of test markers will avoid the pitfall of a 'no further treatment possible' wrongful conclusion.


Assuntos
Cromossomos Humanos Y , Fertilidade/genética , Infertilidade Masculina/genética , Oligospermia/genética , Transtornos do Cromossomo Sexual no Desenvolvimento Sexual/genética , Espermatogênese/genética , Deleção Cromossômica , Cromossomos Humanos Y/genética , Predisposição Genética para Doença , Humanos , Masculino , Reação em Cadeia da Polimerase Multiplex , Oligospermia/diagnóstico , Oligospermia/fisiopatologia , Oligospermia/terapia , Fenótipo , Valor Preditivo dos Testes , Reprodutibilidade dos Testes , Fatores de Risco , Índice de Gravidade de Doença , Aberrações dos Cromossomos Sexuais , Contagem de Espermatozoides , Motilidade Espermática
19.
Hum Reprod ; 32(6): 1192-1201, 2017 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-28444255

RESUMO

STUDY QUESTION: Does lower dose (<26 Gy) cranial radiation therapy (CRT) used for central nervous system prophylaxis in acute lymphoblastic leukemia (ALL) adversely affect sperm concentration or morphology? SUMMARY ANSWER: CRT doses <26 Gy had no demonstrable adverse effect on sperm concentration or morphology. WHAT IS KNOWN ALREADY: Treatment with alkylating agents produces oligospermia and azoospermia in some patients. No prior study has been large enough to evaluate the independent effects of alkylating agents and lower dose (<26 Gy) CRT on sperm concentration or morphology. STUDY DESIGN, SIZE, DURATION: This cross-sectional study included male adult survivors of pediatric ALL who had received alkylating agent chemotherapy with or without CRT and who enrolled in the St. Jude Lifetime Cohort Study (SJLIFE) from September 2007 to October 2013. PARTICIPANTS/MATERIALS, SETTING, METHODS: The inclusion criteria were males, ≥18 years of age, ≥10 years after diagnosis, treated at St. Jude Children's Research Hospital for ALL, and received alkylating agent chemotherapy. Semen analyses were performed on 173 of the 241 (78.1%) adult survivors of pediatric ALL who had received alkylating agent chemotherapy with or without CRT. Cumulative alkylating agent treatment was quantified using the cyclophosphamide equivalent dose (CED). Log-binomial multivariable models were used to calculate relative risks (RRs) and 95% CI. MAIN RESULTS AND THE ROLE OF CHANCE: Compared to those without CRT, risk of oligospermia or azoospermia was not increased for CRT <20 Gy (P = 0.95) or 20-26 Gy (P = 0.58). Participants 5-9 years of age at diagnosis compared to those 0-4 years of age (RR = 1.30, 95% CI, 1.05-.61) or those treated with 8-12 g/m2 CED (RR = 2.06, 95% CI, 1.08-3.94) or ≥12 g/m2 CED (RR = 2.12, 95% CI, 1.09-4.12) compared to those treated with >0 to <4 g/m2 CED had an increased risk for oligospermia or azoospermia. LIMITATIONS, REASONS FOR CAUTION: Our study relied on the results of one semen analysis. ALL survivors who did not participate in SJLIFE or who declined to submit a semen analysis may also have biased our results regarding the proportion with azoospermia or oligospermia, since those who provided a semen specimen were less likely to have previously fathered children compared to those who did not. The lower rate of previous parenthood among participants may have resulted in a higher observed frequency of azoospermia and oligospermia. WIDER IMPLICATIONS OF THE FINDINGS: Treatment with <26 Gy CRT did not increase the risk of oligospermia or azoospermia, although a CED exceeding 8 g/m2 and an age at diagnosis of 5-9 years did increase risk of oligospermia and azoospermia. These findings can be used to counsel adult survivors of pediatric ALL. STUDY FUNDING/COMPETING INTEREST(S): This work was supported by the National Institutes of Health (grant numbers CA 21765, CA 195547, CA00874) and the American Lebanese Syrian Associated Charities (ALSAC). The authors have no competing interests to declare.


Assuntos
Azoospermia/etiologia , Sobreviventes de Câncer , Neoplasias do Sistema Nervoso Central/prevenção & controle , Irradiação Craniana/efeitos adversos , Oligospermia/etiologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/terapia , Lesões por Radiação/epidemiologia , Adulto , Fatores Etários , Antineoplásicos Alquilantes/efeitos adversos , Antineoplásicos Alquilantes/uso terapêutico , Azoospermia/epidemiologia , Azoospermia/fisiopatologia , Neoplasias do Sistema Nervoso Central/secundário , Quimiorradioterapia/efeitos adversos , Estudos de Coortes , Estudos Transversais , Relação Dose-Resposta à Radiação , Seguimentos , Hospitais Pediátricos , Humanos , Masculino , Oligospermia/epidemiologia , Oligospermia/fisiopatologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/tratamento farmacológico , Prevalência , Lesões por Radiação/fisiopatologia , Índice de Gravidade de Doença , Contagem de Espermatozoides , Estados Unidos/epidemiologia
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