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1.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 38(5): 571-575, 2020 Oct 01.
Artigo em Chinês | MEDLINE | ID: mdl-33085244

RESUMO

Low-intensity pulsed ultrasound (LIPUS) is a common physical therapy to accelerate the healing of bone fracture and treat delayed union of bone fracture. Vessels, nerves, and bone tissue are essential constituents of bone system. Recently, increasing evidence has been revealed that LIPUS can not only promote bone regeneration by directly regulating osteoblasts, osteoblasts, mesenchymal stem cells, but also have a positive impact on the repair of bone healing through vessels and nerves. Thus, we reviewed and summarized the latest published literature about the molecular mechanism for the effects of LIPUS on bone regeneration, which might offer a promising therapy for bone-related diseases.


Assuntos
Fraturas Ósseas , Terapia por Ultrassom , Ondas Ultrassônicas , Regeneração Óssea , Consolidação da Fratura , Fraturas Ósseas/terapia , Humanos
2.
Nat Commun ; 11(1): 4907, 2020 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-32999289

RESUMO

Global alterations in the metabolic network provide substances and energy to support tumor progression. To fuel these metabolic processes, extracellular matrix (ECM) plays a dominant role in supporting the mass transport and providing essential nutrients. Here, we report a fibrinogen and thrombin based coagulation system to construct an artificial ECM (aECM) for selectively cutting-off the tumor metabolic flux. Once a micro-wound is induced, a cascaded gelation of aECM can be triggered to besiege the tumor. Studies on cell behaviors and metabolomics reveal that aECM cuts off the mass transport and leads to a tumor specific starvation to inhibit tumor growth. In orthotopic and spontaneous murine tumor models, this physical barrier also hinders cancer cells from distant metastasis. The in vivo gelation provides an efficient approach to selectively alter the tumor mass transport. This strategy results in a 77% suppression of tumor growth. Most importantly, the gelation of aECM can be induced by clinical operations such as ultrasonic treatment, surgery or radiotherapy, implying this strategy is potential to be translated into a clinical combination regimen.


Assuntos
Materiais Biomiméticos/administração & dosagem , Matriz Extracelular/química , Neoplasias/terapia , Animais , Transporte Biológico/efeitos dos fármacos , Transporte Biológico/efeitos da radiação , Materiais Biomiméticos/química , Materiais Biomiméticos/efeitos da radiação , Linhagem Celular Tumoral/transplante , Proliferação de Células/efeitos dos fármacos , Proliferação de Células/efeitos da radiação , Quimiorradioterapia/métodos , Modelos Animais de Doenças , Matriz Extracelular/metabolismo , Matriz Extracelular/efeitos da radiação , Feminino , Fibrinogênio/administração & dosagem , Fibrinogênio/química , Fibrinogênio/efeitos da radiação , Géis , Humanos , Injeções Intravenosas , Metabolômica , Camundongos , Neoplasias/metabolismo , Trombina/administração & dosagem , Trombina/química , Trombina/efeitos da radiação , Terapia por Ultrassom/métodos , Ondas Ultrassônicas
3.
Nat Commun ; 11(1): 4929, 2020 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-33004789

RESUMO

Non-invasive, molecularly-specific, focal modulation of brain circuits with low off-target effects can lead to breakthroughs in treatments of brain disorders. We systemically inject engineered ultrasound-controllable drug carriers and subsequently apply a novel two-component Aggregation and Uncaging Focused Ultrasound Sequence (AU-FUS) at the desired targets inside the brain. The first sequence aggregates drug carriers with millimeter-precision by orders of magnitude. The second sequence uncages the carrier's cargo locally to achieve high target specificity without compromising the blood-brain barrier (BBB). Upon release from the carriers, drugs locally cross the intact BBB. We show circuit-specific manipulation of sensory signaling in motor cortex in rats by locally concentrating and releasing a GABAA receptor agonist from ultrasound-controlled carriers. Our approach uses orders of magnitude (1300x) less drug than is otherwise required by systemic injection and requires very low ultrasound pressures (20-fold below FDA safety limits for diagnostic imaging). We show that the BBB remains intact using passive cavitation detection (PCD), MRI-contrast agents and, importantly, also by sensitive fluorescent dye extravasation and immunohistochemistry.


Assuntos
Barreira Hematoencefálica/metabolismo , Encefalopatias/tratamento farmacológico , Portadores de Fármacos/efeitos da radiação , Agonistas de Receptores de GABA-A/administração & dosagem , Ultrassonografia de Intervenção/métodos , Animais , Barreira Hematoencefálica/diagnóstico por imagem , Barreira Hematoencefálica/efeitos da radiação , Relação Dose-Resposta à Radiação , Portadores de Fármacos/química , Portadores de Fármacos/farmacocinética , Feminino , Agonistas de Receptores de GABA-A/farmacocinética , Humanos , Imagem por Ressonância Magnética , Modelos Animais , Muscimol/administração & dosagem , Muscimol/farmacocinética , Ratos , Técnicas Estereotáxicas , Ondas Ultrassônicas
4.
Ultrasonics ; 108: 106223, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32771811

RESUMO

Quantitative ultrasound can be used to characterize the evolution of the bone-implant interface (BII), which is a complex system due to the implant surface roughness and to partial contact between bone and the implant. The determination of the constitutive law of the BII would be of interest in the context of implant acoustical modeling in order to take into account the imperfect characteristics of the BII. The aim of the present study is to propose an analytical effective model describing the interaction between an ultrasonic wave and a rough BII. To do so, a spring model was considered to determine the equivalent stiffness K of the BII. The stiffness contributions related (i) to the partial contact between the bone and the implant and (ii) to the presence of soft tissues at the BII during the process of osseointegration were assessed independently. K was found to be comprised between 1013 and 1017 N/m3 depending on the roughness and osseointegration of the BII. Analytical values of the reflection and transmission coefficients at the BII were derived from values of K. A good agreement with numerical results obtained through finite element simulation was obtained. This model may be used for future finite element bone-implant models to replace the BII conditions.


Assuntos
Interface Osso-Implante , Osseointegração , Ondas Ultrassônicas , Módulo de Elasticidade , Análise de Elementos Finitos , Modelos Teóricos , Propriedades de Superfície , Resistência à Tração , Titânio
5.
Science ; 369(6504): 638, 2020 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-32764064
6.
J Oleo Sci ; 69(9): 1043-1049, 2020 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-32788510

RESUMO

The 2-ethylhexyl stearate is used as a bio-lubricant in various cosmetic products. The present study is focused on the biocatalyzed esterification of 2-ethylhexanol and stearic acid to form 2-ethylhexyl stearate catalyzed by Fermase CALB 10000 in the presence of ultrasound treatment. The maximum conversion (95.87%) was obtained at molar ratio of 2-ethylhexanol to stearic acid 2:1, enzyme amount of 2 % (w/w), power 80 W, duty cycle 50 % and temperature 50°C in comparatively short reaction time (3 h) in the presence of Fermase as a catalyst. At optimum conditions, it is observed that in the presence of ultrasound; the reaction time minimizes up to 4 h as compared to mechanical stirring method (7 h). The physiochemical properties for the 2-ethylhexyl palmitate were also evaluated.


Assuntos
Hexanóis/química , Estearatos/síntese química , Ondas Ultrassônicas , Biocatálise , Fenômenos Químicos , Enzimas Imobilizadas/química , Esterificação , Estearatos/química , Ácidos Esteáricos/química , Temperatura , Fatores de Tempo
7.
Proc Natl Acad Sci U S A ; 117(29): 17288-17295, 2020 07 21.
Artigo em Inglês | MEDLINE | ID: mdl-32632013

RESUMO

Big brown bats transmit wideband FM biosonar sounds that sweep from 55 to 25 kHz (first harmonic, FM1) and from 110 to 50 kHz (second harmonic, FM2). FM1 is required to perceive echo delay for target ranging; FM2 contributes only if corresponding FM1 frequencies are present. We show that echoes need only the lowest FM1 broadcast frequencies of 25 to 30 kHz for delay perception. If these frequencies are removed, no delay is perceived. Bats begin echo processing at the lowest frequencies and accumulate perceptual acuity over successively higher frequencies, but they cannot proceed without the low-frequency starting point in their broadcasts. This reveals a solution to pulse-echo ambiguity, a serious problem for radar or sonar. In dense, extended biosonar scenes, bats have to emit sounds rapidly to avoid collisions with near objects. But if a new broadcast is emitted when echoes of the previous broadcast still are arriving, echoes from both broadcasts intermingle, creating ambiguity about which echo corresponds to which broadcast. Frequency hopping by several kilohertz from one broadcast to the next can segregate overlapping narrowband echo streams, but wideband FM echoes ordinarily do not segregate because their spectra still overlap. By starting echo processing at the lowest frequencies in frequency-hopped broadcasts, echoes of the higher hopped broadcast are prevented from being accepted by lower hopped broadcasts, and ambiguity is avoided. The bat-inspired spectrogram correlation and transformation (SCAT) model also begins at the lowest frequencies; echoes that lack them are eliminated from processing of delay and no longer cause ambiguity.


Assuntos
Quirópteros/fisiologia , Ecolocação/fisiologia , Som , Animais , Percepção Auditiva/fisiologia , Percepção Espacial/fisiologia , Fatores de Tempo , Ondas Ultrassônicas , Ultrassom
8.
Proc Natl Acad Sci U S A ; 117(29): 16848-16855, 2020 07 21.
Artigo em Inglês | MEDLINE | ID: mdl-32631991

RESUMO

In certain medical applications, transmitting an ultrasound beam through the skin to manipulate a solid object within the human body would be beneficial. Such applications include, for example, controlling an ingestible camera or expelling a kidney stone. In this paper, ultrasound beams of specific shapes were designed by numerical modeling and produced using a phased array. These beams were shown to levitate and electronically steer solid objects (3-mm-diameter glass spheres), along preprogrammed paths, in a water bath, and in the urinary bladders of live pigs. Deviation from the intended path was on average <10%. No injury was found on the bladder wall or intervening tissue.


Assuntos
Cálculos Renais/terapia , Terapia por Ultrassom/métodos , Ondas Ultrassônicas , Animais , Suínos , Transdutores , Terapia por Ultrassom/instrumentação
9.
PLoS One ; 15(6): e0229891, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32497076

RESUMO

A facile method has been developed for the synthesis of Schiff bases derived from substituted and unsubstituted 3-amino- and 4-amino-1,2,4-triazoles. Condensation of the aminotrizoles with a variety of aromatic aldehydes afforded desired Schiff bases in excellent yields in 3-5 minutes of exposure to ultra-sound. The synthesized compounds were characterized by means of IR, 1HNMR and Mass spectrometry. The synthesized compounds were also screened for their antibacterial potential against Gram-negative (Escherichia coli, Shigella sonnei, Pseudomonas aeruginosa and Salmonella typhi) and two Gram-positive (Staphylococcus aureus and Bacillus subtilis) strains.


Assuntos
Amitrol (Herbicida)/síntese química , Amitrol (Herbicida)/farmacologia , Antibacterianos/síntese química , Antibacterianos/farmacologia , Triazóis/síntese química , Triazóis/farmacologia , Ondas Ultrassônicas , Amitrol (Herbicida)/química , Antibacterianos/química , Bactérias/efeitos dos fármacos , Técnicas de Química Sintética , Testes de Sensibilidade Microbiana , Bases de Schiff/química , Triazóis/química
10.
Chemosphere ; 257: 127121, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32512327

RESUMO

In this paper, a hybrid advanced oxidation process of sonoelectrochemical, in which ultrasound and electrochemical are applied simultaneously, has been used for the degradation of ofloxacin (bio-recalcitrant pharmaceutical pollutant). Response surface methodology based central composite design was applied to understand the parametric effects of ultrasonic power, current density, initial pH, and electrolyte dose. Enhanced ofloxacin degradation was obtained using sonoelectrochemical (≈95%) process in comparison to the electrochemical (≈60.6%) and sonolysis alone (≈7.2%) after 120 min treatment time. Multi-response optimization was used so as to maximize COD removal (70.12%) and minimize specific energy consumption (11.92 kWh (g COD removed)-1)at the optimized parametric condition of pH = 6.3 (natural pH), ultrasonic power = 54 W, current density = 213 A m-2, and Na2SO4 electrolyte dose = 2.0 g L-1. It was revealed that •OH radicals contribute major to the ofloxacin degradation reaction among the other oxidizing agents. Degradation of the ofloxacin followed pseudo-first-order kinetics with a higher reaction rate, which confirmed the synergistic effect of 34% between ultrasound and electrochemical approaches. The degradation pathway of ofloxacin removal was elucidated at optimum condition by the temporal evolution of the intermediate compounds and final products using gas chromatography coupled with mass spectroscopy (GC-MS), liquid chromatography-mass spectroscopy (LC-MS), high-resolution mass spectroscopy (HR-MS), and Fourier transform infrared spectroscopy (FTIR). Atomic force microscopy (AFM) and field emission scanning electron microscope (FE-SEM) coupled with energy dispersed X-ray (EDX) were used to determine the morphology of electrodes. Operational cost analysis was done based on the reactor employed in the present study.


Assuntos
Ofloxacino/química , Poluentes Químicos da Água/química , Técnicas Eletroquímicas , Eletrodos , Cromatografia Gasosa-Espectrometria de Massas , Cinética , Ofloxacino/análise , Oxirredução , Ondas Ultrassônicas , Poluentes Químicos da Água/análise
11.
Int J Nanomedicine ; 15: 3771-3790, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32547027

RESUMO

Introduction: Rapamycin has been considered as a potential treatment for osteoarthritis (OA). Drug carriers fabricated from liposomes can prolong the effects of drugs and reduce side effects of drugs. Low-intensity pulsed ultrasound (LIPUS) has been found to possess anti-OA effects. Materials and Methods: The anti-osteoarthritic effects of liposome-encapsulated rapamycin (L-rapa) combined with LIPUS were examined by culture of normal and OA chondrocytes in alginate beads and further validated in OA prone Dunkin-Hartley guinea pigs. Results: L-rapa with LIPUS largely up-regulated aggrecan and type II collagen mRNA in human OA chondrocytes (HOACs). L-rapa with LIPUS caused significant enhancement in proteoglycan and type II collagen production in HOACs. Large decreases in both MMP-13 and IL-6 proteins were found in the HOACs exposed to L-rapa with LIPUS. Intra-articular injection of 40 µL L-rapa at both 5 µM and 50 µM twice a week combined with LIPUS thrice a week for 8 weeks significantly increased GAGs and type II collagen in the cartilage of knee. Results on OARSI score showed that intra-articular injection of 5 µM L-rapa with LIPUS displayed the greatest anti-OA effects. Immunohistochemistry revealed that L-rapa with or without LIPUS predominantly reduced MMP-13 in vivo. The values of complete blood count and serum biochemical examinations remained in the normal ranges after the injections with or without LIPUS. These data indicated that intra-articular injection of L-rapa collaborated with LIPUS is not only effective against OA but a safe OA therapy. Conclusion: Taken together, L-rapa combined with LIPUS possessed the most consistently and effectively anabolic and anti-catabolic effects in HOACs and the spontaneous OA guinea pigs. This study evidently revealed that liposome-encapsulation collaborated with LIPUS is able to reduce the effective dose and administration frequency of rapamycin and further stably reinforce its therapeutic actions against OA.


Assuntos
Osteoartrite/terapia , Sirolimo/uso terapêutico , Ondas Ultrassônicas , Animais , Peso Corporal/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Proliferação de Células/efeitos da radiação , Células Cultivadas , Condrócitos/efeitos dos fármacos , Condrócitos/patologia , Condrócitos/efeitos da radiação , Colágeno Tipo II/metabolismo , Liberação Controlada de Fármacos , Cobaias , Humanos , Injeções Intra-Articulares , Interleucina-6/metabolismo , Lipossomos/ultraestrutura , Masculino , Metaloproteinase 13 da Matriz/metabolismo , Pessoa de Meia-Idade , Osteoartrite/sangue , Osteoartrite/patologia , Proteoglicanas/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Sirolimo/administração & dosagem , Sirolimo/farmacologia
12.
J Vis Exp ; (160)2020 06 13.
Artigo em Inglês | MEDLINE | ID: mdl-32597853

RESUMO

Stereotaxic surgery is the gold standard for localized drug and gene delivery to the rodent brain. This technique has many advantages over systemic delivery including precise localization to a target brain region and reduction of off target side effects. However, stereotaxic surgery is highly invasive which limits its translational efficacy, requires long recovery times, and provides challenges when targeting multiple brain regions. Focused ultrasound (FUS) can be used in combination with circulating microbubbles to transiently open the blood brain barrier (BBB) in millimeter sized regions. This allows intracranial localization of systemically delivered agents that cannot normally cross the BBB. This technique provides a noninvasive alternative to stereotaxic surgery. However, to date this technique has yet to be widely adopted in neuroscience laboratories due to the limited access to equipment and standardized methods. The overall goal of this protocol is to provide a benchtop approach to FUS BBB opening (BBBO) that is affordable and reproducible and can therefore be easily adopted by any laboratory.


Assuntos
Barreira Hematoencefálica/metabolismo , Sistemas de Liberação de Medicamentos/métodos , Ondas Ultrassônicas , Animais , Laboratórios , Microbolhas , Ratos
13.
J Environ Manage ; 266: 110616, 2020 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-32392147

RESUMO

Furfural is a toxic compound that can cause many problems for human health and the environment. In this study, we addressed the degradation of furfural in aqueous solution using the activated persulfate (SPS) and peroxymonosulfate (PMS) through the ultrasonic (US) wave. Besides, the effect of various parameters (pH, oxidizing dose, initial furfural concentration, US frequency, Inorganic anions concentration, and scavenger) on SPS + US (SPS/US) and PMS + US (PMS/US) processes were examined. The results showed, in order to furfural removal, the US had excellent efficiency in activating SPS and PMS, as in SPS/US and PMS/US processes, 95.3% and 58.4% of furfural (at 25 mg/L concentration) was decomposed in 90 min, respectively. The furfural degradation rate increased with increasing oxidizing dose and US frequency in both SPS/US and PMS/US processes. Considering the synergistic effect, the best removal rate has occurred in the SPS/US process. In the SPS/US and PMS/US processes, furfural removal increased at natural pH (pH 7), and the presence of inorganic anions such as NO3- and Cl- had negative effects on furfural removal efficiency. Also CO32- and HCO3- acted as a radical scavenger in the SPS/US process but these anions in the PMS/US process produced more SO4-° radicals, and subsequently, they increased the furfural degradation rate. The results also showed that the predominant radical in the oxidation reactions is the sulfate radical. This study showed that the SPS/US and PMS/US processes are promising methods for degrading organic pollutants in the environment.


Assuntos
Ondas Ultrassônicas , Poluentes Químicos da Água , Furaldeído , Oxirredução , Peróxidos
14.
Anticancer Res ; 40(5): 2549-2557, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32366399

RESUMO

BACKGROUND/AIM: Sonodynamic cancer therapy is based on the preferential uptake and/or retention of a sonosensitizing drug (sonosensitizer) in tumor tissues and the subsequent activation of the drug by ultrasound irradiation. In the present study, we investigated the sonodynamically-induced antitumoral effect with functionalized carbon nanotubes, such as poly-ethylene glycol-modified carbon nanotubes (PEG-modified CNTs). MATERIALS AND METHODS: Antitumor effects were evaluated using histological observation and assessing tumor growth following sonodynamic exposure to PEG-modified CNTs. RESULTS: The combined treatment of 100 µM PEG-modified CNT and ultrasound induced a 2-fold cytotoxicity. Sodium azide, which quenches singlet oxygen, significantly inhibited ultrasonication induced cell damage in the presence of PEG-modified CNTs. This suggests that singlet oxygen produced by the combined use of PEG-modified CNTs and ultrasound is involved in the induction of antitumoral effects. The destruction of tumor tissue was observed with the ultrasonic treatment in combination with PEG-modified CNTs, while neither the treatment with PEG-modified CNTs alone nor ultrasound alone caused any necrosis. CONCLUSION: These results indicate that PEG-modified CNT functions as a sonosensitizer and is effective for sonochemical treatment of solid tumors.


Assuntos
Antineoplásicos/química , Antineoplásicos/farmacologia , Nanotubos de Carbono , Polietilenoglicóis , Ondas Ultrassônicas , Animais , Antineoplásicos/isolamento & purificação , Linhagem Celular Tumoral , Modelos Animais de Doenças , Humanos , Masculino , Camundongos , Estrutura Molecular , Nanotubos de Carbono/química , Polietilenoglicóis/química , Espécies Reativas de Oxigênio/metabolismo , Sarcoma 180 , Terapia por Ultrassom , Ensaios Antitumorais Modelo de Xenoenxerto
15.
PLoS One ; 15(5): e0232617, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32438389

RESUMO

Photoaging, the premature aging of skin induced by ultraviolet rays, is characterized by wrinkling, roughness, laxity, and pigmentary changes. Various natural and synthetic retinoids have been explored for the treatment of aging. Among retinoids, adapalene (Ada, 0.3%) is one of the most potent and widely used drugs to treat photoaging. However, it causes irritant reactions that limit its acceptance by patients. Several studies have shown the applicability of Lysozyme (Lys)-shelled microbubbles (MBs) for drug delivery through sonophoresis, and recently we have shown its efficiency to treat inflammatory skin disease. Here, we report the construction of novel Ada-LysMBs based on opposite electric charges for combined effects to treat photoaging. The Ada-LysMBs were self-assembled and had a mean diameter of 2857 nm. The maximum loading efficiency of Ada onto LysMBs was 13.99 ± 0.59%. An acoustic power density of 3 W/cm2 for 1 min revealing maximum penetration depth of LysMBs was optimized for further in vitro and in vivo studies of Ada-LysMBs. It was observed that in vitro Ada release from Ada-LysMBs at 6 h after ultrasound (US) treatment was more rapid at pH 7.4 (82%) than at pH 5.5 (73%). Franz diffusion experiments on isolated porcine skin indicated that US approximately doubled Ada delivery by Ada-LysMBs and Ada + LysMBs at 12 h and six-fold Lys permeation by LysMBs at 6 h, compared to these treatments alone. A 5-week in vivo study in mice identified significant wrinkle reduction in animals treated with US plus Ada-LysMBs. Our findings indicate that US may be used with Ada-LysMBs in the water phase to treat photoaging by normalizing hyperkeratinization and promoting collagen synthesis.


Assuntos
Adapaleno/administração & dosagem , Fármacos Dermatológicos/administração & dosagem , Sistemas de Liberação de Medicamentos , Microbolhas/uso terapêutico , Muramidase/administração & dosagem , Retinoides/administração & dosagem , Envelhecimento da Pele/efeitos dos fármacos , Ondas Ultrassônicas , Adapaleno/farmacologia , Animais , Feminino , Camundongos , Camundongos Endogâmicos BALB C , Muramidase/farmacologia , Retinoides/farmacologia , Suínos , Raios Ultravioleta/efeitos adversos
16.
Proc Natl Acad Sci U S A ; 117(23): 12674-12685, 2020 06 09.
Artigo em Inglês | MEDLINE | ID: mdl-32430322

RESUMO

Robust cytotoxic T cell infiltration has proven to be difficult to achieve in solid tumors. We set out to develop a flexible protocol to efficiently transfect tumor and stromal cells to produce immune-activating cytokines, and thus enhance T cell infiltration while debulking tumor mass. By combining ultrasound with tumor-targeted microbubbles, membrane pores are created and facilitate a controllable and local transfection. Here, we applied a substantially lower transmission frequency (250 kHz) than applied previously. The resulting microbubble oscillation was significantly enhanced, reaching an effective expansion ratio of 35 for a peak negative pressure of 500 kPa in vitro. Combining low-frequency ultrasound with tumor-targeted microbubbles and a DNA plasmid construct, 20% of tumor cells remained viable, and ∼20% of these remaining cells were transfected with a reporter gene both in vitro and in vivo. The majority of cells transfected in vivo were mucin 1+/CD45- tumor cells. Tumor and stromal cells were then transfected with plasmid DNA encoding IFN-ß, producing 150 pg/106 cells in vitro, a 150-fold increase compared to no-ultrasound or no-plasmid controls and a 50-fold increase compared to treatment with targeted microbubbles and ultrasound (without IFN-ß). This enhancement in secretion exceeds previously reported fourfold to fivefold increases with other in vitro treatments. Combined with intraperitoneal administration of checkpoint inhibition, a single application of IFN-ß plasmid transfection reduced tumor growth in vivo and recruited efficacious immune cells at both the local and distant tumor sites.


Assuntos
Imunoterapia/métodos , Interferon beta/genética , Neoplasias Experimentais/terapia , Linfócitos T/imunologia , Transfecção/métodos , Ondas Ultrassônicas , Animais , Linhagem Celular Tumoral , Membrana Celular/efeitos da radiação , Movimento Celular , Humanos , Interferon beta/metabolismo , Camundongos , Microbolhas/uso terapêutico , Linfócitos T/fisiologia
17.
Zhonghua Yi Xue Za Zhi ; 100(18): 1432-1436, 2020 May 12.
Artigo em Chinês | MEDLINE | ID: mdl-32392996

RESUMO

Objective: To study the efficacy and safety of low-intensity pulsed ultrasound (LIPUS) at different intervals by mechanical force in treating erectile dysfunction (ED). Method: Forty patients with mild to moderate ED were randomized in a 1∶1 ratio to receive 16-treatment sessions of LIPUS in group A and group B, applied 3 times per week and 2 times per week, respectively. End-point assessments were made at 8th week after treatment. Efficacy were evaluated using International Index of Erectile Function-Erectile Function domain score (IIEF-EF), Erectile Hardness Score (EHS), Self-Esteem and Relationship Questionnaire (SEAR), Sexual Encounter Profile (SEP), Global Assessment Question (GAQ), and pain were assessed by Visual Analogue Score (VAS).Treatment response was confirmed by a minimal clinically importance difference (MCID) at 8th week. Results: Compared with baseline, IIEF-EF score [(17.1±5.48 vs 23.4±3.75, P<0.05) and (18.9±4.34 vs 24.1±4.32, P<0.05)], proportion of EHS 4 [(0 vs 40%, P<0.05) and (16.7% vs 55.6%, P<0.05)], and Overall Relationship score [(50.6 vs 67.5, P<0.05) and (44.4 vs 70.1, P<0.05)] were significantly improved at 8th week in two groups, respectively. Compared with baseline, the positive responses to SEP-3 increased significantly at 8th week in two groups (50.0% vs 80.0%,P<0.05) and (44.4% vs 88.9%, P<0.05), respectively. The positive responses to GAQ-2 were 90.0% and 88.9% at 8th week in two groups, respectively. There were no significant differences in IIEF-EF, EHS, SEAR, SEP and GAQ at 8th week between two groups. There was no significant difference in treatment response using MCID between two groups at end-point (80.5% vs 77.5%). The treatment duration for full sessions were 2.5 weeks less in group A than group B. No adverse effects were reported in all cases. Conclusion: LIPUS at two different intervals is effective and safe for mild to moderate ED, and the regimen at 3 times per week can achieve quite good effect in relatively short duration,while the long-term effects is still be clarified in further study.


Assuntos
Disfunção Erétil , Ondas Ultrassônicas , Método Duplo-Cego , Disfunção Erétil/terapia , Humanos , Masculino , Ereção Peniana , Resultado do Tratamento , Terapia por Ultrassom
18.
Food Chem ; 326: 127026, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32438231

RESUMO

This study investigates the effect of flat sweep frequency and pulsed ultrasound (FSFPU) pretreatment prior to vacuum pulsation (VP) drying on water migration and quality attributes of okra pods. The results showed that the moisture diffusion was the fastest in VP drying process when FSFPU pretreatment parameters were set as 40 kHz, 25.0 W/L, sweep period 18-180 ms and amplitude ± 1 kHz. In addition, FSFPU pretreatment caused the change of microstructure (villi and xylem), resulting in significant decrease of drying time required. The VP dried okra pods with FSFPU pretreatment had features: i) increased total phenolic contents and antioxidant capacity; ii) no significant changes of total flavonoids contents; iii) a lower chlorophyll degradation (13.5% less), higher hardness and frangibility, and a lower total color change (reduced browning index); iv) reduced negative effects on the flavor. Therefore, the use of FSFPU pretreatment prior to VP drying is suggested.


Assuntos
Abelmoschus/química , Ondas Ultrassônicas , Antioxidantes/química , Dessecação , Flavonoides/química , Fenóis/química , Vácuo
19.
J Oleo Sci ; 69(6): 585-595, 2020 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-32404549

RESUMO

The extraction of peanut oil was investigated using the combination of ultrasound and heat application, which is known as a novel technology called thermosonication. The study was set up using the Box-Behnken design and the models based on quadratic equations were established. The effects of extraction time (4-12 min), extraction temperature (40-60°C), solvent-to-solid ratio (SSR) (3:1-9:1)(v/w) and ultrasound power (60-100%) on the extraction yield and the oleic acid concentration of extracted oils were investigated. Results showed that the extraction yield was primarily affected by the extraction temperature and SSR. The average maximum yield of 39.93% was achieved when variables were set to 12 min of time, 50°C of temperature, 9:1(v/w) of SSR and 80% of ultrasound power. Thermosonication did not significantly affect the fatty acid composition. Since it was targeted to determine an optimum point where the maximum extraction yield and oleic acid concentration were obtained, a multiobjective optimization was performed. The optimum thermosonication conditions were determined as 4 min of time, 60°C of temperature, 9:1(v/w) of SSR and 100% of power with a maximum extraction yield of 39.86%. Also, the oleic acid concentration was determined as 63.51% in this optimum condition.


Assuntos
Manipulação de Alimentos/métodos , Temperatura Alta , Extração Líquido-Líquido/métodos , Óleo de Amendoim/química , Óleo de Amendoim/isolamento & purificação , Temperatura , Ondas Ultrassônicas , Ácido Oleico/análise , Fatores de Tempo
20.
Phys Med Biol ; 65(12): 125017, 2020 06 22.
Artigo em Inglês | MEDLINE | ID: mdl-32460260

RESUMO

Nanocarriers offer a promising approach to significantly improve therapeutic delivery to solid tumors as well as limit the side effects associated with anti-cancer agents. However, their relatively large size can negatively affect their ability to efficiently penetrate into more interior tumor regions, ultimately reducing therapeutic efficacy. Poor penetration of large agents such as nanocarriers is attributed to factors in the tumor microenvironment such as elevated interstitial fluid pressure (IFP) and fibrillar collagen in the extracellular matrix. Our previous studies reported that pretreatment of solid tumor xenografts with nondestructive pulsed focused ultrasound (pFUS) can improve the delivery and subsequent therapy of a variety of therapeutic formulations in different tumor models, where the results were associated with expanded extracellular spaces (ECS), an increase in hydraulic conductivity, and decrease in tissue stiffness. Here, we demonstrate the inverse relationship between IFP and the penetration of systemically administered nanoparticle (NP) probes, where IFP increased from the tumor periphery to their center. Furthermore, we show that pretreatment with pFUS can safely reduce IFP and improve NP delivery; especially into the center of the tumors. These results coincide with effects generated in the fibrillar collagen network microstructure in the ECS as determined by quantitative polarized light microscopy. Whole tumor and histomorphometric analysis, however, did not show significant differences in collagen area fraction or collagen feature solidity, as well as tumor cross-sectional area and aspect ratio, as a result of the treatments. We present a biophysical model connecting the experimental results, where pFUS-mediated cytoarchitectural changes are associated with improved redistribution of the interstitial fluid and lower IFP. The resulting improvement in NP delivery supports our previous therapeutic studies and may have implications for clinical applications to improve therapeutic outcomes in cancer therapy.


Assuntos
Transformação Celular Neoplásica , Líquido Extracelular/metabolismo , Nanopartículas/metabolismo , Pressão , Carcinoma de Células Escamosas de Cabeça e Pescoço/patologia , Ondas Ultrassônicas , Animais , Transporte Biológico , Humanos
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