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1.
J Zoo Wildl Med ; 50(2): 405-413, 2019 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-31260207

RESUMO

Snake fungal disease (SFD) is an emerging mycotic disease caused by Ophidiomyces ophiodiicola, and has been demonstrated to impact snake populations of conservation concern in the United States negatively. Although Ophidiomyces has been shown to affect diverse taxa and to have a broad distribution, host factors associated with infected individuals and optimal testing protocols are not yet well characterized. The purpose of this study was to evaluate host factors and agreement across testing modalities associated with Ophidiomyces infection in a free-ranging snake population in southeast Ohio. Wild-caught snakes were swabbed and biopsied to test for Ophidiomyces via quantitative polymerase chain reaction (qPCR), culture, and histopathology. The host parameters assessed were species, sex, snout-vent length, body weight, month captured, and presence of gross skin lesions. A total of 8/30 individuals across three species-Black Racers (Coluber constrictor), Grey Ratsnakes (Pantherophis spiloides), and Eastern Gartersnakes (Thamnophis sirtalis sirtalis)-tested positive via at least one testing modality for Ophidiomyces infection. There were no associations between sex, snout-vent length, or weight and Ophidiomyces infection status. A higher proportion of individuals with gross lesions tested positive for Ophidiomyces than those without gross lesions, and most individuals that tested positive were caught in April or May. A low level of agreement was observed across testing modalities. Swab qPCR identified the most Ophidiomyces-positive individuals, and fungal culture identified the fewest at 0 individuals. Although there are limitations associated with a sample size of 30, these findings support the potential of this pathogen to infect individuals broadly across species and size, highlighting the relevance of this disease for snake conservation efforts. They also suggest that although little agreement was observed across test modalities, the concurrent use of multiple modalities is a more sensitive method for characterizing prevalence and distribution of Ophidiomyces.


Assuntos
Dermatomicoses/veterinária , Onygenales/isolamento & purificação , Serpentes , Animais , Doenças Transmissíveis Emergentes/epidemiologia , Doenças Transmissíveis Emergentes/microbiologia , Doenças Transmissíveis Emergentes/veterinária , Dermatomicoses/epidemiologia , Dermatomicoses/microbiologia , Ohio/epidemiologia , Pele/microbiologia
2.
J Zoo Wildl Med ; 50(1): 225-230, 2019 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-31120682

RESUMO

Nannizziopsis crocodili, a contagious, keratinophilic fungus, was identified from biopsied tissue in a captive juvenile freshwater crocodile during an outbreak of severe multifocal dermatitis affecting four of five crocodiles. Lesions progressed from superficial, well-demarcated ulceration of scales, to black pigmentation, localized edema, erythema, and flattening of the scales. Treatment with topical enilconazole provided clinical improvement in three of four crocodiles but all developed terminal gout. One crocodile did not develop clinical disease despite long-term exposure. This is the first report of N. crocodili in freshwater crocodiles and in a location remote to the index Australian case.


Assuntos
Jacarés e Crocodilos , Dermatite/veterinária , Fungicidas Industriais/uso terapêutico , Imidazóis/uso terapêutico , Micoses/veterinária , Onygenales/isolamento & purificação , Animais , Animais de Zoológico , Dermatite/diagnóstico , Dermatite/tratamento farmacológico , Dermatite/microbiologia , Micoses/diagnóstico , Micoses/tratamento farmacológico , Micoses/microbiologia
3.
Ecohealth ; 16(1): 141-150, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30349999

RESUMO

Snake fungal disease (SFD) is an emerging disease caused by the fungal pathogen, Ophidiomyces ophiodiicola. Clinical signs of SFD include dermal lesions, including regional and local edema, crusts, and ulcers. Snake fungal disease is widespread in the Eastern United States, yet there are limited data on how clinical signs of SFD compare with laboratory diagnostics. We compared two sampling methods for O. ophiodiicola, scale clip collection and swabbing, to evaluate whether collection method impacted the results of polymerase chain reaction (PCR). In addition, we evaluated the use of clinical signs to predict the presence of O. ophiodiicola across seasons, snake habitat affiliation (aquatic or terrestrial) and study sites. We found no significant difference in PCR results between sampling methods. Clinical signs were a strong predictor of O. ophiodiicola presence in spring and summer seasons. Snakes occupying terrestrial environments had a lower overall probability of testing positive for O. ophiodiicola compared to snakes occupying aquatic environments. Although our study indicates that both clinical signs of SFD and prevalence of O. ophiodiicola vary seasonally and based on habitat preferences of the host, our analysis suggests that clinical signs can serve as a reliable indicator of O. ophiodiicola presence, especially during spring and summer.


Assuntos
Dermatomicoses/veterinária , Onygenales/isolamento & purificação , Serpentes/microbiologia , Animais , Kentucky/epidemiologia , Reação em Cadeia da Polimerase
4.
Med Mycol ; 57(7): 825-832, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-30520962

RESUMO

We report several cases of fungal infections in snakes associated with a new species within the genus Paranannizziopsis. Three juvenile Wagler's vipers (Tropidolaemus wagleri) presented with skin abnormalities or ulcerative dermatitis, and two snakes died. Histologic examination of skin from the living viper revealed hyperplastic, hyperkeratotic, and crusting epidermitis with intralesional fungal elements. The terrestrial Wagler's vipers were housed in a room with fully aquatic tentacled snakes (Erpeton tentaculatum), among which there had been a history of intermittent skin lesions. Approximately 2 months after the biopsy of the viper, a skin sample was collected from one tentacled snake (TS1) with skin abnormalities and revealed a fungal infection with a similar histologic appearance. Fungal isolates were obtained via culture from the Wagler's viper and TS1 and revealed a novel species, Paranannizziopsis tardicrescens, based on phenotypic characterization and molecular analysis. P. tardicrescens was cultured and identified by DNA sequence analysis 8 months later from a dead tentacled snake in an exhibit in an adjacent hallway and 13 months later from a living rhinoceros snake (Rhynchophis boulengeri) with two focal skin lesions. Antifungal susceptibility testing on three of four cultured isolates demonstrated potent in vitro activity for terbinafine and voriconazole.


Assuntos
Micoses/veterinária , Onygenales/isolamento & purificação , Pele/microbiologia , Serpentes/microbiologia , Animais , Biópsia , Feminino , Masculino , Micoses/mortalidade , Onygenales/classificação , Pele/patologia
5.
Mycologia ; 110(2): 325-338, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29852093

RESUMO

Ascosphaera apis is an intestinally infective, spore-forming, filamentous fungus that infects honeybees and causes deadly chalkbrood disease. Although A. apis has been known for 60 y, little is known about the ultrastructure of the spores. In this study, the fine morphology and ultrastructure of an isolate, A. apis CQ1 from southwest China, was comprehensively identified by transmission electron microscopy, confocal laser scanning microscopy, scanning electron microscopy, and optical microscopy. The high sequence similarity and phylogenetic data based on nuc rDNA ITS1-5.8S-ITS2 (ITS) supported the hypothesis that the CQ1 strain is a new member of the A. apis species. Morphological observation indicated that the mature spores are long ovals with an average size of 2 × 1.2 µm and are tightly packed inside spherical spore balls. More than 10 spore balls that were 8-16 µm in diameter were wrapped and formed a spherical, nearly hyaline spore cyst of 50-60 µm in diameter. Ultrastructural analysis showed that mature spores have two nuclei with distinctly different sizes. A large nucleus with double nuclear membranes was found in the center of the spore, whereas the small nucleus was only one-fifth of the large nucleus volume and was located near the end of the spore. Numerous ribosomes filled the cytoplasm, and many mitochondria with well-defined structures were arranged along the inner spore wall. The spore wall consists of an electron-dense outer surface layer, an electron-lucent layer, and an inner plasma membrane. Chitin is the major component of the spore wall. The germinated spore was observed as an empty spore coat, whereas the protoplasts, including the nuclei, mitochondria, and ribosomes, had been discharged. In addition to these typical fungal spore organelles, an unknown electron-dense regular structure might be the growing mycelium, which was arranged close to the inner spore wall and almost covered the entire wall area.


Assuntos
Abelhas/microbiologia , Onygenales/citologia , Onygenales/ultraestrutura , Esporos Fúngicos/citologia , Esporos Fúngicos/ultraestrutura , Animais , Parede Celular/química , China , Quitina/análise , Análise por Conglomerados , DNA Fúngico/química , DNA Fúngico/genética , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Microscopia , Microscopia Confocal , Microscopia Eletrônica de Transmissão , Onygenales/classificação , Onygenales/isolamento & purificação , Organelas/ultraestrutura , Filogenia , RNA Ribossômico 5,8S , Análise de Sequência de DNA
7.
PLoS Negl Trop Dis ; 12(1): e0006174, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-29357352

RESUMO

Emergomyces africanus is a thermally dimorphic fungus that causes a systemic mycosis in immunocompromised persons in South Africa. Infection is presumed to follow inhalation of airborne propagules. We developed a quantitative PCR protocol able to detect as few as 5 Es. africanus propagules per day. Samples were collected in Cape Town, South Africa over 50 weeks by a Burkard spore trap with an alternate orifice. We detected Es. africanus in air samples from 34 days (10%) distributed over 11 weeks. These results suggest environmental exposure to airborne Es. africanus propagules occurs more commonly in endemic areas than previously appreciated.


Assuntos
Microbiologia do Ar , Técnicas Microbiológicas/métodos , Onygenales/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real/métodos , Onygenales/genética , África do Sul
8.
Med Mycol ; 56(4): 510-513, 2018 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-28992307

RESUMO

The ecological niche of Emergomyces africanus (formerly Emmonsia species), a dimorphic fungus that causes an AIDS-related mycosis in South Africa, is unknown. We hypothesized that natural infection with E. africanus occurs in wild small mammals. Using molecular detection with primers specific for E. africanus, we examined 1402 DNA samples from 26 species of mole-rats, rodents, and insectivores trapped in South Africa that included 1324 lung, 37 kidney, and 41 liver specimens. DNA of E. africanus was not detected in any animals. We conclude that natural infection of wild small mammals in South Africa with E. africanus has not been proven.


Assuntos
Micoses/microbiologia , Onygenales/genética , Animais , DNA Espaçador Ribossômico/genética , Humanos , Mamíferos/microbiologia , Técnicas Microbiológicas , Onygenales/isolamento & purificação , África do Sul
9.
Mycopathologia ; 182(11-12): 967-978, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28894998

RESUMO

The new species Spiromastigoides albida (Onygenales, Eurotiomycetes, Ascomycota), from a lung biopsy in USA, is proposed and described based on morphological data and the analysis of rRNA, and fragments of actin and ß-tubulin gene sequences. This species is characterized by white colonies and a malbranchea-like asexual morph with profusely branching curved conidiophores forming sporodochia-like structures. Moreover, new combinations for Gymnoascus alatosporus, and for some new species recently described under the generic name Spiromastix, are provided.


Assuntos
Pulmão/microbiologia , Micoses/microbiologia , Onygenales , Biópsia , DNA Fúngico/genética , Humanos , Micoses/diagnóstico , Onygenales/classificação , Onygenales/genética , Onygenales/isolamento & purificação , Filogenia , RNA Ribossômico/genética , Esporos Fúngicos/classificação
10.
Mycoses ; 60(7): 469-476, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28321920

RESUMO

From stratum corneum samples of a palmar eczema, a fungus was isolated that developed white colonies with a yellowish dark reverse, suggestive of dermatophytes. The isolate produced numerous chlamydospores and sparse aleuroconidia, was resistant to cycloheximide, grew well on human stratum corneum samples and was positive in tests for urease production and hair perforation, but no dermatophyte could be identified. After several weeks, cleistothecia with delicate asci and disc-shaped ascospores were formed, suggesting Arachnomyces spp. The analyses of the ribosomal ITS and LSU (D1/D2 domains) nucleotide sequences proved a good match with the ex-type strain of Xanthothecium peruvianum (family Onygenaceae, order Onygenales), and LSU sequence showed 99% similarity with Arachnomyces glareosus. This is the first report of X. peruvianum isolated from human skin. The description of our isolate provides new information about this species and proposes its transfer to the genus Arachnomyces with the subsequent emendation of the description of Arachnomyces peruvianus. Morphologically and physiologically it mimics dermatophytes and other species of the genus Arachnomyces. Although the clinical situation did not suggest any relevance for A. peruvianus as a primary pathogen, this fungus may act as a secondary pathogen under suitable conditions due to its keratinolytic capacity.


Assuntos
Epiderme/microbiologia , Onygenales/classificação , Onygenales/isolamento & purificação , Adulto , Análise por Conglomerados , DNA Fúngico/química , DNA Fúngico/genética , DNA Ribossômico/química , DNA Ribossômico/genética , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Eczema/microbiologia , Humanos , Masculino , Técnicas Microbiológicas , Onygenales/genética , Onygenales/crescimento & desenvolvimento , Filogenia , RNA Ribossômico/genética , Análise de Sequência de DNA
11.
Mycoses ; 60(5): 310-319, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28240390

RESUMO

A novel dimorphic fungus, Emergomyces orientalis sp. nov. a close relative of systemic pathogens in the family Ajellomycetaceae (Blastomyces, Histoplasma). The fungus is reported in a 64-year-old male from Shanxi, China. The patient developed disseminated skin lesions, productive cough with fever and showed nodular opacities in his left lung on chest radiography. The patient had no identified cause of immunodeficiency apart from type-2 diabetes mellitus. Clinical, histopathological and mycological characteristics of the agent are given, and its phylogenetic position is determined with multilocus sequence data.


Assuntos
Infecções Fúngicas Invasivas/diagnóstico , Infecções Fúngicas Invasivas/microbiologia , Onygenales/isolamento & purificação , Onygenales/patogenicidade , Filogenia , Blastomyces/genética , China , DNA Ribossômico , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/microbiologia , Febre/etiologia , Febre/microbiologia , Histoplasma/genética , Humanos , Pulmão/diagnóstico por imagem , Pulmão/microbiologia , Pulmão/patologia , Masculino , Pessoa de Meia-Idade , Tipagem de Sequências Multilocus , Onygenales/classificação , Onygenales/genética , Análise de Sequência de DNA , Pele/microbiologia , Pele/patologia , Tomografia Computadorizada por Raios X
12.
N Z Vet J ; 64(5): 298-300, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27079795

RESUMO

AIMS: To describe the methods used at the Animal Health Laboratory (AHL, Ministry for Primary Industries) to identify Paranannizziopsis australasiensis. METHODS: Skin biopsy samples from two adult male tuatara were submitted to the AHL in March 2014. Approximately half of each sample was processed for fungal culture and incubated on mycobiotic agar containing cycloheximide at 30°C. Following morphological examination of the culture products, DNA was extracted from suspect colonies. PCR was used to amplify the internal transcribed spacer (ITS) region of fungal rRNA using primers ITS1 and ITS4. Positive amplicons were subjected to DNA sequencing and the results were compared to published sequences. In addition, DNA was extracted from the remaining skin samples and the same PCR was carried out to compare the results. RESULTS: After 7 days of incubation, colonies morphologically resembling P. australasiensis were observed. DNA extracted from these isolates tested positive for P. australasiensis by PCR and DNA sequencing. Samples of DNA extracted directly from the infected skin samples tested negative for P. australasiensis using the generic fungal PCR. CONCLUSIONS AND CLINICAL RELEVANCE: Isolation and identification of P. australasiensis was carried out using a combination of fungal culture and molecular testing available at AHL. Results were available in significantly less time than in the past, when isolates had to be sent overseas. PCR and sequencing of fungal isolates is a valuable tool for identification of species that have few, if any, unique macroscopic or microscopic features to aid identification. Further sampling from captive and wild New Zealand reptiles will provide important information on the epidemiology of P. australasiensis, and the conservation and management implications for tuatara and other native reptile species.


Assuntos
Dermatomicoses/veterinária , Onygenales/genética , Répteis/microbiologia , Animais , Sequência de Bases , DNA Fúngico/genética , Dermatomicoses/microbiologia , Masculino , Onygenales/isolamento & purificação , Reação em Cadeia da Polimerase/veterinária , Pele/microbiologia
13.
Rev. iberoam. micol ; 33(1): 51-54, ene.-mar. 2016. ilus
Artigo em Inglês | IBECS | ID: ibc-149376

RESUMO

Background. Blastomycosis is a subacute or chronic deep mycosis caused by a dimorphic fungus called Blastomyces dermatitidis, which generally produces a pulmonary form of the disease and, to a lesser extent, extra-pulmonary forms such as cutaneous, osteoarticular and genitourinary, among others. Cutaneous blastomycosis is the second clinical presentation in frequency. It is considered as primary when it begins by inoculation of the fungus due to traumas, and secondary when the lung fails to contain the infection. Case-report. We present the case of a 57 year-old male who had a 5 year-history of an irregularly shaped verrucous infiltrative plaque related to and insect bite and posterior trauma due to the manipulation of the lesion. B. dermatitidis was identified using direct examination, stains, isolation in culture media, histopathology, and molecular studies. An antifungal susceptibility test was performed using method M38-A2 (CLSI). Clinical and mycological cure was achieved with itraconazole. Conclusions. This cutaneous blastomycosis case acquired in the United States (Indianapolis) is rather interesting and looks quite similar to other mycoses such as coccidioidomycosis or sporotrichosis. The presented case shows one of the multiple issues concerning migration between neighboring countries (AU)


Antecedentes. La blastomicosis es una micosis profunda, subaguda o crónica, causada por el hongo dimorfo Blastomyces dermatitidis, que generalmente produce una enfermedad pulmonar y, en menor proporción, formas extrapulmonares (cutánea, osteoarticular y genitourinaria). La blastomicosis cutánea es la segunda forma clínica más observada; es de tipo primario cuando se presenta por la inoculación del hongo a través de traumatismos, y de tipo secundario cuando se disemina a partir de un foco pulmonar. Caso clínico. Se presenta el caso de un varón de 57 años con blastomicosis cutánea verrugosa de 5 años de evolución, relacionada con la picadura de un insecto, que empeora con la manipulación de la lesión. El caso fue confirmado por la identificación de B. dermatitidis en exámenes directos, tinciones, aislamiento en medios de cultivo habituales, análisis histopatológico y estudios moleculares. Se realizó una prueba de sensibilidad antifúngica por el método M38-A2 (CLSI). La curación clínica y micológica se logró con itraconazol oral. Conclusiones. Este caso de blastomicosis cutánea adquirida en Estados Unidos (Indianápolis) es de gran interés y resulta similar a los de otras micosis, como la coccidioidomicosis y la esporotricosis. Este caso refleja uno de los múltiples factores relacionados con la migración entre países cercanos (AU)


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Blastomicose/diagnóstico , Blastomicose/tratamento farmacológico , Blastomicose/microbiologia , Itraconazol/uso terapêutico , Blastomyces , Blastomyces/isolamento & purificação , Onygenales , Onygenales/isolamento & purificação , Prednisona/uso terapêutico , Testes de Sensibilidade Microbiana/métodos , Testes de Sensibilidade Microbiana , Sensibilidade e Especificidade
14.
J Wildl Dis ; 52(1): 143-9, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26745835

RESUMO

Snake fungal disease (SFD) is an emerging disease of wildlife believed to be caused by Ophidiomyces ophiodiicola. Although geographic and host ranges have yet to be determined, this disease is characterized by crusty scales, superficial pustules, and subcutaneous nodules, with subsequent morbidity and mortality in some snake species. To confirm the presence of SFD and O. ophiodiicola in snakes of eastern Virginia, US, we clinically examined 30 free-ranging snakes on public lands from April to October 2014. Skin biopsy samples were collected from nine snakes that had gross lesions suggestive of SFD; seven of these biopsies were suitable for histologic interpretation, and eight were suitable for culture and PCR detection of O. ophiodiicola. Seven snakes had histologic features consistent with SFD and eight were positive for O. ophiodiicola by PCR or fungal culture.


Assuntos
Micoses/veterinária , Onygenales/isolamento & purificação , Serpentes , Animais , Feminino , Masculino , Micoses/microbiologia , Micoses/patologia , Onygenales/genética , Onygenales/patogenicidade , Pele/microbiologia , Pele/patologia , Virginia
15.
Hautarzt ; 67(9): 739-49, 2016 Sep.
Artigo em Alemão | MEDLINE | ID: mdl-26758910

RESUMO

Moulds or non-dermatophyte moulds (NDM) are being increasingly isolated as causative agent of onychomycoses. Known causes of a NDM-OM are Scopulariopsis brevicaulis, Fusarium, Aspergillus, Acremonium, Neoscytalidium dimidiatum, Arthrographis kalrae, and Chaetomium. In this article, 5 patients with suspected nail infection due to Onychocola canadensis are reported for the first time in Germany. Systemic antifungal agents are not considered to be effective in NDM onychomycosis. In individual cases, however, terbinafine seems to be effective in Onychocola canadensis infection of the nails. Treatment of choice represents, however, nontraumatic nail avulsion using 40 % urea ointment followed by antifungal nail lacquer with ciclopirox olamine or amorolfine.


Assuntos
Naftalenos/uso terapêutico , Onicomicose/diagnóstico , Onicomicose/tratamento farmacológico , Onygenales/isolamento & purificação , Idoso , Antifúngicos/uso terapêutico , Doenças Transmissíveis Emergentes/diagnóstico , Doenças Transmissíveis Emergentes/tratamento farmacológico , Doenças Transmissíveis Emergentes/microbiologia , Feminino , Alemanha , Humanos , Masculino , Pessoa de Meia-Idade , Onicomicose/microbiologia , Terbinafina , Resultado do Tratamento
16.
PLoS One ; 10(6): e0130560, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26102072

RESUMO

Bees are essential pollinators for many flowering plants, including agriculturally important crops such as apple. As geographic ranges of bees or their host plants change as a result of human activities, we need to identify pathogens that could be transmitted among newly sympatric species to evaluate and anticipate their effects on bee communities. We used PCR screening and DNA sequencing to evaluate exposure to potentially disease-causing microorganisms in a pollinator of apple, the horned mason bee (Osmia cornifrons). We did not detect microsporidia, Wolbachia, or trypanosomes, which are common pathogens of bees, in any of the hundreds of mason bees screened. We did detect both pathogenic and apathogenic (saprophytic) fungal species in the genus Ascosphaera (chalkbrood), an unidentified species of Aspergillus fungus, and a strain of bacteria in the genus Paenibacillus that is probably apathogenic. We detected pathogenic fungal strains in asymptomatic adult bees that therefore may be carriers of disease. We demonstrate that fungi from the genus Ascosphaera have been transported to North America along with the bee from its native range in Japan, and that O. cornifrons is exposed to fungi previously only identified from nests of other related bee species. Further study will be required to quantify pathogenicity and health effects of these different microbial species on O. cornifrons and on closely-related native North American mason bees that may now be exposed to novel pathogens. A global perspective is required for pathogen research as geographic ranges of insects and microorganisms shift due to intentional or accidental introductions.


Assuntos
Aspergillus/isolamento & purificação , Abelhas/microbiologia , Espécies Introduzidas , Onygenales/isolamento & purificação , Polinização , Migração Animal , Animais , Aspergillus/classificação , Aspergillus/genética , Aspergillus/patogenicidade , Abelhas/classificação , DNA Bacteriano/análise , DNA Fúngico/análise , Feminino , Interações Hospedeiro-Patógeno , Inseticidas , Japão , Funções Verossimilhança , Masculino , Malus , Dados de Sequência Molecular , Comportamento de Nidação , New York , Onygenales/classificação , Onygenales/genética , Onygenales/patogenicidade , Paenibacillus/classificação , Paenibacillus/genética , Paenibacillus/isolamento & purificação , Paenibacillus/patogenicidade , Filogenia , Alinhamento de Sequência , Homologia de Sequência do Ácido Nucleico , Especificidade da Espécie
17.
Mycoses ; 58(2): 113-7, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25591072

RESUMO

We report the first case of onychomycosis caused by Onychocola canadensis in Estonia. We believe that the number of nail infections caused by this fungus is underestimated due to the current diagnostic algorithm of non-dermatophytic onychomycosis. The need to define categories and criteria for 'proven' and 'probable' non-dermatophyte mold infections to promote more extensive studies in the future is also discussed.


Assuntos
Dermatoses do Pé/microbiologia , Fungos Mitospóricos/isolamento & purificação , Onicomicose/microbiologia , Onygenales/isolamento & purificação , Estônia , Feminino , Humanos , Pessoa de Meia-Idade , Fungos Mitospóricos/citologia , Onygenales/citologia
18.
Med Mycol ; 51(6): 614-24, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23470038

RESUMO

Auxarthron is a genus within the Onygenales encompassing keratinophilic species with typical ascomata (gymnothecia) consisting of anastomosing network of thick-walled hyphae and small globose or oblate ascospores. No association of this genus with clinically relevant cases of human or animal infection has been reported. This paper describes the isolation of an undescribed Auxarthron species as an agent of proven onychomycosis affecting almost all fingernails in a man with psoriasis. The causality of the isolated fungus was verified by repeated sampling and direct microscopy revealing irregular septate hyphae. Based on micro- and macromorphological features and unique sequence data (ITS region, benA and RPB2 gene), the isolated fungus is proposed as the new species A. ostraviense. The sibling species of A. ostraviense, A. umbrinum, was isolated from three patients with suspected onychomycosis and a detailed clinical history is provided for one of these patients. All four isolates were tested for susceptibility to selected antifungal agents. Terbinafine and clotrimazole appear to be effective in vitro. The morphological identification of Auxarthron spp. is non-trivial, time-consuming and requires cultivation media other than Sabouraud glucose agar which is routinely used in dermatomycology.


Assuntos
Onicomicose/microbiologia , Onygenales/isolamento & purificação , Adulto , Antifúngicos/farmacologia , Clotrimazol/farmacologia , DNA Fúngico/química , DNA Fúngico/genética , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Feminino , Proteínas Fúngicas/genética , Humanos , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Dados de Sequência Molecular , Naftalenos/farmacologia , Onygenales/classificação , Onygenales/genética , Psoríase/complicações , Análise de Sequência de DNA , Terbinafina
19.
Med Mycol ; 51(2): 113-20, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22852752

RESUMO

A new fungal genus and species, Aphanoascella galapagosensis, recovered from carapace keratitis in a Galapagos tortoise residing in a south Texas zoological collection, is characterized and described. The presence of a pale peridium composed of textura epidermoidea surrounded by scarce Hülle cell-like chlamydospores, and the characteristic reticulate ascospores with an equatorial rim separates it from other genera within the Onygenales. The phylogenetic tree inferred from the analysis of D1/D2 sequences demonstrates that this fungus represents a new lineage within that order. As D1/D2 and ITS sequence data also shows a further separation of Aphanoascus spp. into two monophyletic groups, we propose to retain the generic name Keratinophyton for species whose ascospores are pitted and display a conspicuous equatorial rim, and thereby propose new combinations in this genus for four Aphanoascus species.


Assuntos
Ceratite/veterinária , Onygenales/classificação , Tartarugas/microbiologia , Animais , Sequência de Bases , DNA Fúngico/química , DNA Fúngico/genética , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Ceratite/microbiologia , Ceratite/patologia , Dados de Sequência Molecular , Onygenales/citologia , Onygenales/genética , Onygenales/isolamento & purificação , Filogenia , Alinhamento de Sequência , Análise de Sequência de DNA , Especificidade da Espécie , Esporos Fúngicos , Texas
20.
Braz. j. microbiol ; 43(1): 247-252, Jan.-Mar. 2012. ilus, tab
Artigo em Inglês | LILACS | ID: lil-622810

RESUMO

The past few decades have witnessed an overwhelming increase in the incidence of fungal infections, particularly in immunocompromised individuals. Consequently, zoonotic diseases, especially through rodents constitute a prominent group among the emerging diseases. Rodents are commensal to man and related health risks are common. Water rats (Rattus norvegicus) are typical to Vembanadu-Kol wetland agroecosystems, where they can act as a good carrier nexus for pathogens. The present study evaluates the carrier status of water rats with respect to fungal pathogens. A total of fifty two fungi covering eighteen families were isolated. Among the isolates, eight were dermaptophytes and Chrysosporium sp. (89.18%) was the frequent isolate. The source-wise analyses showed an increased isolation from ventral hair (67 isolates). Water rats of Vembanadu-Kol wetland agroecosystem are potent carrier of dermaptophytes and other opportunistic fungi, and strong carrier paths are existing too.


Assuntos
Humanos , Animais , Ratos , Antifúngicos/análise , Chrysosporium/isolamento & purificação , Fungos Mitospóricos/isolamento & purificação , Pneumopatias Fúngicas , Onygenales/isolamento & purificação , Doenças dos Roedores , Roedores , Agricultura Sustentável , Fungos Aquáticos , Metodologia , Áreas Alagadas , Áreas Alagadas
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