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1.
Med Sci Monit ; 26: e923343, 2020 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-32281613

RESUMO

BACKGROUND The presence of a Frank's sign ear crease is closely correlated with coronary artery disease (CAD). The SYNTAX score indicates the complexity of coronary lesions. This present investigation sought to identify the correlation between SYNTAX score and several specific ear creases. MATERIAL AND METHODS Four specific types of ear creases - crossing crease not originated from ear hole (CC-NEH), crossing crease originated from ear hole (CC-EH), vertical creases on the face side (VC-F), and vertical creases dividing earlobe and face (VC-EF) - were investigated in patients undergoing coronary angiography. A Frank's sign score system was introduced based on the 4 creases. Demographic data, clinical data, and SYNTAX score were also documented. The association between ear creases and SYNTAX score, as well as the correlation between Frank's score and SYNTAX score, were statistically analyzed. RESULTS CC-NEH had the highest positive predictive value (positive predictive value=0.439), and VC-F had the highest negative predictive value for the detection of intermediate and high SYNTAX score (negative predictive value=1.000). VC-EF and CC-NEH were associated with intermediate and high SYNTAX scores (OR=2.913-7.694, all P<0.05). Only 2.9% of patients with Frank's score=0 had intermediate or high SYNTAX scores, and 52.2% and 50.0% of patients with Frank's sign score=3 and 4 had intermediate or high SYNTAX scores, respectively. The Frank's sign score was significantly and positively correlated with SYNTAX score (r=0.457, P<0.001). CONCLUSIONS Features of specific ear creases and Frank's sign scores were associated with intermediate and high complexity of coronary lesions.


Assuntos
Doença da Artéria Coronariana/patologia , Orelha/anatomia & histologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Angiografia Coronária , Estudos Transversais , Feminino , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Sensibilidade e Especificidade , Adulto Jovem
2.
Int. j. morphol ; 38(1): 147-152, Feb. 2020. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1056413

RESUMO

Dog ear is very important because of disease vulnerability. Therefore, gross anatomy and sectional anatomy on CT and MRI of the dog ear should be mastered by veterinarian. The purpose of this research was to present the digital atlases which high quality sectioned images and 3D models of detailed structures of dog ear could be displayed freely. In the sectioned images of a female beagle, ear structures were reconstructed by surface modeling to make 3D models. The sectioned images and 3D models were put into the browsing software and PDF file, respectively. Using the browsing software and the PDF file, gross and radiological anatomy of dog ear could be learned easily and accurately. The auditory tube of a dog was placed anterior to the tympanic cavity unlike human. The tensor tympani muscle of a dog was connected to the dorsal wall of the tympanic cavity with the malleus. No remarkable difference in the auditory ossicles, semicircular ducts, facial nerve, and endolymphatic duct was observed between dogs and humans. The software and the PDF file will be provided to other researchers freely to help contribute to veterinary research and education.


La oreja del perro es importante debido a la vulnerabilidad de enfermedad. Por lo tanto, el veterinario debe conocer plenamente la anatomía macroscópica y la anatomía seccional en la TC y la RM del oído del perro. El objetivo de esta investigación fue presentar los atlas digitales que podían mostrar imágenes seccionadas de alta calidad y modelos 3D de estructuras detalladas de orejas de perro. En las imágenes seccionadas de una hembra Beagle, las estructuras de las orejas se reconstruyeron mediante modelado de superficie con el objetivo de crear modelos 3D. Las imágenes seccionadas y los modelos 3D se colocaron en un software de navegación y un archivo PDF. El uso de software de navegación y el archivo PDF permiten un aprendizaje fácil y preciso de la anatomía macroscópica y radiológica de la oreja de perro. El músculo tensor del tímpano de un perro estaba conectado a la pared dorsal de la cavidad timpánica con el martillo. No se observaron diferencias notables en los huesecillos auditivos, los conductos semicirculares, el nervio facial y el conducto endolinfático entre perros y humanos. El software y el archivo PDF estarán disponibles libremente para los investigadores para ayudar en la investigación y educación veterinaria.


Assuntos
Animais , Cães , Orelha/diagnóstico por imagem , Software , Anatomia Transversal , Imageamento Tridimensional , Orelha/anatomia & histologia , Projetos Ser Humano Visível , Pavilhão Auricular/diagnóstico por imagem
3.
BMC Microbiol ; 20(1): 22, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31996131

RESUMO

BACKGROUND: Staphylococcus aureus is a human pathogen that is a common cause of nosocomial infections and infections on indwelling medical devices, mainly due to its ability to shift between the planktonic and the biofilm/sessile lifestyle. Biofilm infections present a serious problem in human medicine as they often lead to bacterial persistence and thus to chronic infections. The immune responses elicited by biofilms have been described as specific and ineffective. In the few experiments performed in vivo, the importance of neutrophils and macrophages as a first line of defence against biofilm infections was clearly established. However, the bilateral interactions between biofilms and myeloid cells remain poorly studied and analysis of the dynamic processes at the cellular level in tissues inoculated with biofilm bacteria is still an unexplored field. It is urgent, therefore, to develop biologically sound experimental approaches in vivo designed to extract specific immune signatures from the planktonic and biofilm forms of bacteria. RESULTS: We propose an in vivo transgenic mouse model, used in conjunction with intravital confocal microscopy to study the dynamics of host inflammatory responses to bacteria. Culture conditions were created to prepare calibrated inocula of fluorescent planktonic and biofilm forms of bacteria. A confocal imaging acquisition and analysis protocol was then drawn up to study the recruitment of innate immune cells in the skin of LysM-EGFP transgenic mice. Using the mouse ear pinna model, we showed that inflammatory responses to S. aureus can be quantified over time and that the dynamics of innate immune cells after injection of either the planktonic or biofilm form can be characterized. First results showed that the ability of phagocytic cells to infiltrate the injection site and their motility is not the same in planktonic and biofilm forms of bacteria despite the cells being considerably recruited in both cases. CONCLUSION: We developed a mouse model of infection to compare the dynamics of the inflammatory responses to planktonic and biofilm bacteria at the tissue and cellular levels. The mouse ear pinna model is a powerful imaging system to analyse the mechanisms of biofilm tolerance to immune attacks.


Assuntos
Orelha/microbiologia , Interações entre Hospedeiro e Microrganismos/imunologia , Otite/imunologia , Pele , Infecções Cutâneas Estafilocócicas/imunologia , Staphylococcus aureus , Animais , Biofilmes , Modelos Animais de Doenças , Feminino , Masculino , Camundongos , Camundongos Transgênicos , Otite/microbiologia , Pele/imunologia , Pele/microbiologia , Staphylococcus aureus/patogenicidade , Staphylococcus aureus/fisiologia
4.
PLoS Negl Trop Dis ; 14(1): e0006596, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31923234

RESUMO

An association between increased susceptibility to infectious diseases and obesity has been described as a result of impaired immunity in obese individuals. It is not clear whether a similar linkage can be drawn between obesity and parasitic diseases. To evaluate the effect of obesity in the immune response to cutaneous Leishmania major infection, we studied the ability of C57BL/6 mice fed a hypercaloric diet (HSB) to control leishmaniasis. Mice with diet-induced obesity presented thicker lesions with higher parasite burden and a more intense inflammatory infiltrate in the infected ear after infection with L. major. There was no difference between control and obese mice in IFN-gamma or IL-4 production by auricular draining lymph node cells, but obese mice produced higher levels of IgG1 and IL-17. Peritoneal macrophages from obese mice were less efficient to kill L. major when infected in vitro than macrophages from control mice. In vitro stimulation of macrophages with IL-17 decreased their capacity to kill the parasite. Moreover, macrophages from obese mice presented higher arginase activity. To confirm the role of IL-17 in the context of obesity and infection, we studied lesion development in obese IL-17R-/- mice infected with L. major and found no difference in skin lesions and the leukocyte accumulation in the draining lymph node is redcuced in knockout mice compared between obese and lean animals. Our results indicate that diet-induced obesity impairs resistance to L. major in C57BL/6 mice and that IL-17 is involved in lesion development.


Assuntos
Leishmania major/patogenicidade , Leishmaniose Cutânea/imunologia , Obesidade , Animais , Dieta/efeitos adversos , Orelha/parasitologia , Feminino , Interferon gama , Interleucina-17 , Leishmaniose Cutânea/parasitologia , Linfonodos/citologia , Macrófagos Peritoneais/parasitologia , Camundongos Endogâmicos C57BL , Camundongos Knockout , Fatores de Risco
5.
Int J Syst Evol Microbiol ; 70(1): 83-88, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31596191

RESUMO

Auritidibacter ignavus is a Gram-stain-positive bacillus derived from otorrhea. Four strains derived from ear discharges in Canada and Switzerland, with features consistent with but distinguishable from Auritidibacter ignavus IMMIB L-1656T (accession number FN554542) by 16S rRNA gene sequencing (97.5 % similarity), were thought to represent a novel species of the genus Auritidibacter. Auritidibacter ignavus DSM 45359T (=IMMIB L-1656T) was acquired to compare with Canadian and Swiss strains by whole-genome sequencing (WGS). Unexpectedly, those isolates were observed to be consistent with A. ignavus DSM 45359T by WGS (ANIb scores >98 %), MALDI-TOF (Bruker), cellular fatty acid analysis and biochemically (some differences were observed). A nearly full 16S rRNA gene sequence could not be readily prepared from A. ignavus DSM 45359T, even after multiple attempts. A 16S rRNA gene chimeric consensus sequence created from the genome assembly of A. ignavus DSM 45359T had only 97.5 % similarity to that of A. ignavus IMMIB L-1656T, implying that 16S rRNA sequence accession number FN554542 could not be replicated. We concluded that our isolates of members of the genus Auritidibacter were consistent with A. ignavus DSM 45359T, did not represent a novel species, and that the sequence corresponding to FN554542 was not reproducible. By WGS, A. ignavus DSM 45359T had genome of 2.53×106 bp with a DNA G+C content of 59.34%, while genomes of Canadian and Swiss isolates ranged from 2.47 to 2.59×106 bp with DNA G+C contents of 59.3-59.52 %. A. ignavus NML 100628 (=NCTC 14178=LMG 30897) did not demonstrate a rodcoccus cycle. Emendation of Auritidibacter ignavus was proposed based on these results.


Assuntos
Micrococcaceae/classificação , Filogenia , Idoso , Técnicas de Tipagem Bacteriana , Composição de Bases , Canadá , DNA Bacteriano/genética , Orelha/microbiologia , Ácidos Graxos/química , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Suíça
6.
Meat Sci ; 159: 107934, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31493738

RESUMO

The aim of this pilot study was to develop and evaluate an automated system for the assessment of ear and tail lesions as welfare indicators in pigs at the abattoir. A camera-based system was constructed to enable automatic assessment. This was evaluated in comparison to a standard observer, defined as skilled human veterinary observer, based on 5952 carcasses. We detected significant values for ear lesions (sensitivity, 77.0; specificity, 96.5; accuracy, 95.4%) and good values for tail lesions (sensitivity, 77.8%; specificity, 99.7%; accuracy, 99.5%). The reliabilities of assessments by four individual human observers directly at the abattoir and the camera system were calculated using Krippendorff's alpha. These varied between 0.42 and 0.67 for ear lesions and 0.42 and 0.55 for tail lesions, while better values were reached for the agreement between the system and picture-based evaluation (0.64 for ear lesions and 0.75 for tail lesions). More scientific evaluations at different abattoirs are now needed to further test and improve the camera-based system and its various scopes of application.


Assuntos
Bem-Estar do Animal , Automação , Orelha/patologia , Suínos , Cauda/patologia , Ferimentos e Lesões/veterinária , Matadouros , Animais , Processamento de Imagem Assistida por Computador , Projetos Piloto , Ferimentos e Lesões/diagnóstico
7.
J Matern Fetal Neonatal Med ; 33(1): 57-61, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29860925

RESUMO

Background: The Bilicare™ is a new device that measures transcutaneous bilirubin (TcB) level at the ear pinna. There are only few studies which have evaluated its accuracy in clinical practice.Objective: This study aims to determine the accuracy of Bilicare™ as a predischarge screening tool in late preterm and term neonates and to define the optimal cutoff point for determining the need to measure total serum bilirubin (TSB).Methods: The 35 weeks' gestation or more and healthy neonates who underwent predischarge TSB measurement were enrolled. Bilicare™ TcB was measured within 30 minutes of blood sampling. Paired TcB and TSB data were analyzed.Results: We collected 214 paired samples. Mean age (SD) at TcB measurement was 57.17 (7.47) hours. Mean TSB (SD) was 9.79 (2.83) mg/dL. TcB showed a significant correlation with TSB (r = 0.84, r2 = 0.7). The mean difference (SD) between TcB and TSB was 0.7 (0.21) mg/dL (95%CI 0.49-0.91). TcB tended to overestimate TSB level at the TSB values of <12 mg/dL but underestimate at the higher TSB level. The accuracy of using TcB values to detect neonates who required phototherapy was 92.5%. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were 78.3, 94.2, 62.1, and 97.3%, respectively. If TcB +3 mg/dL was applied as a cutoff point, the sensitivity, specificity, PPV, and NPV were 100, 53.9, 20.7, and 100%, respectively.Conclusions: Bilicare™ TcB and TSB measurements were well correlated. The TcB level +3 mg/dL could detect all neonates who had significant hyperbilirubinemia requiring phototherapy during their birth hospitalization.


Assuntos
Bilirrubina/sangue , Hiperbilirrubinemia Neonatal/diagnóstico , Triagem Neonatal/instrumentação , Alta do Paciente , Pele/diagnóstico por imagem , Bilirrubina/análise , Estudos Transversais , Orelha , Feminino , Idade Gestacional , Humanos , Hiperbilirrubinemia Neonatal/sangue , Recém-Nascido , Doenças do Prematuro/sangue , Doenças do Prematuro/diagnóstico , Masculino , Triagem Neonatal/métodos , Triagem Neonatal/normas , Nascimento Prematuro/sangue , Estudos Prospectivos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Pele/metabolismo , Nascimento a Termo/sangue , Fatores de Tempo
8.
Nature ; 576(7785): 44-45, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31792418
9.
Cell Mol Biol (Noisy-le-grand) ; 65(7): 99-104, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31880525

RESUMO

Inflammation and insomnia are medical problems that may severely affect work and health, thereby necessitating strategies for their effective treatment. Icartin (ICT) is a major active monomeric component of icariin  . Studies have revealed that ICT possesses several pharmacological properties such anti-inflammatory, anti-tumor, anti-fibrotic, anti-osteoporotic and neuroprotective effects. The present research was carried out to investigate the anti-inflammatory, analgesic and sedative/hypnotic effects of ICT. The results obtained revealed that ICT exerted a good anti-inflammatory effect related to the downregulations of inflammatory cytokines and the inhibition of COX-2 signaling pathway. Moreover, ICT enhanced Cl- influx in mouse cortical cells in a concentration-dependent manner. These data suggest that ICT exerts a hypnotic effect in mice through a mechanism associated with increased Cl- influx in cortical cells.


Assuntos
Analgésicos/uso terapêutico , Anti-Inflamatórios/uso terapêutico , Encéfalo/metabolismo , Cloretos/metabolismo , Flavonoides/uso terapêutico , Hipnóticos e Sedativos/uso terapêutico , Inflamação/tratamento farmacológico , Animais , Encéfalo/efeitos dos fármacos , Ciclo-Oxigenase 2/metabolismo , Orelha/patologia , Feminino , Inflamação/induzido quimicamente , Masculino , Camundongos , Pentobarbital/uso terapêutico , Transdução de Sinais/efeitos dos fármacos , Sono/efeitos dos fármacos , Latência do Sono/efeitos dos fármacos , Xilenos/toxicidade
10.
Zhongguo Zhong Yao Za Zhi ; 44(22): 4857-4863, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31872593

RESUMO

To prepare Helix aspersa muller-paeonol nanogel( PAE-HAM-Gels) with anti-proliferative scar effect,evaluate its skin penetration,retention and irritation,and to investigate its prevention and treatment effect for hypertrophic scar in rabbit ears. The dermal retention,transdermal rate and cumulative permeability of paeonol were investigated in vitro by using the modified Franz diffusion cell and the abdominal skin of suckling pigs,SD rats and KM mice,respectively,and the in vitro permeation curves were drawn. The normal skin of the back of New Zealand rabbits was continuously treated with PAE-HAM-Gels for 7 days,and the physiological state of the skin was observed under light microscope after HE staining by using homologous left and right contrast method. The hypertrophic scar model in rabbit ears was established,and the New Zealand rabbits were randomly divided into blank group,model group,positive drug group,PAE-Gels group and PAE-HAM-Gels group. After 28 days of administration,the scar hyperplasia rate and scar elevation index( SEI) of each group were calculated; the scar tissues were taken and stained with Masson for observation of collagen fibers and muscle fibers hyperplasia under light microscope,and the expression level of TGF-ß1 in each group was detected. The Qnof PAE-HAM-Gels in aqueous solution was in line with the Higuchi equation,and its transdermal rate,cumulative permeation and dermal retention in different animal skins were all higher than those of PAE-Gels. The skin of the drug-administered group was intact,without erythema,edema or other phenomena; under light microscope,the subcutaneous tissue and the epidermal cells were neatly arranged with uniform thickness,which showed no difference from the blank group. The scar hyperplasia rate of the PAE-HAM-Gels group was 62. 50%; SEI was 2. 17±0. 33 and TGF-ß1 was( 815. 4±34. 69) ng·L~(-1),significantly different from those in model group( P<0. 01). Masson staining showed that as compared with the model group,the number of collagen fibers and muscle fibers was small and the arrangement was loose and tidy in the PAE-HAM-Gels group,with regular arrangement of chondrocytes and a small number of inflammatory cells and microvessels.PAE-HAM-Gels have good transdermal properties and dermal retention without skin irritation,offering a promising therapeutic strategy for transdermal delivery during the prevention and treatment of hypertrophic scar in rabbit ears.


Assuntos
Acetofenonas/química , Cicatriz Hipertrófica , Orelha , Nanogéis/química , Animais , Camundongos , Coelhos , Ratos , Ratos Sprague-Dawley , Suínos
11.
Genes (Basel) ; 10(10)2019 10 12.
Artigo em Inglês | MEDLINE | ID: mdl-31614864

RESUMO

In an era of unprecedented global change, exploring patterns of gene expression among wild populations across their geographic range is crucial for characterizing adaptive potential. RNA-sequencing studies have successfully characterized gene expression differences among populations experiencing divergent environmental conditions in a wide variety of taxa. However, few of these studies have identified transcriptomic signatures to multivariate, environmental stimuli among populations in their natural environments. Herein, we aim to identify environmental and sex-driven patterns of gene expression in the Tasmanian devil (Sarcophilus harrisii), a critically endangered species that occupies a heterogeneous environment. We performed RNA-sequencing on ear tissue biopsies from adult male and female devils from three populations at the extremes of their geographic range. There were no transcriptome-wide patterns of differential gene expression that would be suggestive of significant, environmentally-driven transcriptomic responses. The general lack of transcriptome-wide variation in gene expression levels across the devil's geographic range is consistent with previous studies that documented low levels of genetic variation in the species. However, genes previously implicated in local adaptation to abiotic environment in devils were enriched for differentially expressed genes. Additionally, three modules of co-expressed genes were significantly associated with either population of origin or sex.


Assuntos
Variação Genética , Marsupiais/genética , Transcriptoma/genética , Adaptação Biológica/genética , Animais , Análise por Conglomerados , Biologia Computacional , Orelha , Espécies em Perigo de Extinção , Feminino , Regulação da Expressão Gênica , Ontologia Genética , Masculino , População/genética
12.
J Stroke Cerebrovasc Dis ; 28(12): 104348, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31570261

RESUMO

BACKGROUND: Sensory impairment is associated with reduced functional recovery in stroke survivors. Invasive vagus nerve stimulation (VNS) paired with rehabilitative interventions improves motor recovery in chronic stroke. Noninvasive approaches, for example, transcutaneous auricular VNS (taVNS) are safe, well-tolerated and may also improve motor function in those with residual weakness. We report the impact of taVNS paired with a motor intervention, repetitive task practice, on sensory recovery in a cohort of patients with chronic stroke. METHODS: Twelve participants who were more than 3 months postischemic stroke with residual upper limb weakness received 18 × 1 hour sessions over 6 weeks with an average of at least 300 repetitions of functional arm movements per session concurrently with taVNS at maximum tolerated intensity. Light touch and proprioception were scored as part of the Upper Limb Fugl-Meyer (UFM) assessment at baseline and postintervention (score range for sensation 0-12). RESULTS: Eleven participants (92%) had sensory impairment at baseline of whom 7 (64%) regained some sensation (proprioception n = 6 participants, light touch n = 2, both modalities n = 1) postintervention. The maximal increase in UFM sensation score (3 points) was seen in the patient with the greatest improvement in motor function. CONCLUSIONS: taVNS paired with motor rehabilitation may improve sensory recovery in chronic stroke patients. The relative contribution of motor and sensory rehabilitation to overall functional recovery in chronic stroke needs further characterization in a larger, phase 2 study.


Assuntos
Terapia por Exercício , Atividade Motora , Sensação , Reabilitação do Acidente Vascular Cerebral/métodos , Acidente Vascular Cerebral/terapia , Estimulação Elétrica Nervosa Transcutânea , Extremidade Superior/inervação , Estimulação do Nervo Vago , Idoso , Doença Crônica , Orelha , Estudos de Viabilidade , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Plasticidade Neuronal , Propriocepção , Recuperação de Função Fisiológica , Acidente Vascular Cerebral/diagnóstico , Acidente Vascular Cerebral/fisiopatologia , Fatores de Tempo , Tato , Percepção do Tato , Resultado do Tratamento
13.
Nat Commun ; 10(1): 4432, 2019 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-31570755

RESUMO

The pathway of homeostatic IgG extravasation is not fully understood, in spite of its importance for the maintenance of host immunity, the management of autoantibody-mediated disorders, and the use of antibody-based biologics. Here we show in a murine model of pemphigus, a prototypic cutaneous autoantibody-mediated disorder, that blood-circulating IgG extravasates into the skin in a time- and dose-dependent manner under homeostatic conditions. This IgG extravasation is unaffected by depletion of Fcγ receptors, but is largely attenuated by specific ablation of dynamin-dependent endocytic vesicle formation in blood endothelial cells (BECs). Among dynamin-dependent endocytic vesicles, IgG co-localizes well with caveolae in cultured BECs. An Abl family tyrosine kinase inhibitor imatinib, which reduces caveolae-mediated endocytosis, impairs IgG extravasation in the skin and attenuates the murine pemphigus manifestations. Our study highlights the kinetics of IgG extravasation in vivo, which might be a clue to understand the pathological mechanism of autoantibody-mediated autoimmune disorders.


Assuntos
Imunoglobulina G/imunologia , Pênfigo/imunologia , Pênfigo/metabolismo , Proteínas Tirosina Quinases/metabolismo , Pele/imunologia , Animais , Autoanticorpos/metabolismo , Doenças Autoimunes/metabolismo , Cavéolas , Modelos Animais de Doenças , Dinaminas/metabolismo , Orelha/patologia , Endocitose , Células Endoteliais/metabolismo , Feminino , Imunoglobulina G/sangue , Cinética , Camundongos , Camundongos Endogâmicos C57BL , Pênfigo/patologia , Pele/patologia , Vesículas Transportadoras/metabolismo
14.
Acta Cir Bras ; 34(8): e201900804, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31618404

RESUMO

PURPOSE: To develop a rabbit model of a short peripheral catheter (SPC) and to observe the effects of different flushing methods on blood vessels. METHODS: Thirty rabbits were randomly divided into three groups (A, B, and C), with ten rabbits per group. In group A, we used pulsed flush; in group B, we used uniform flush; and no treatment was used in group C. RESULTS: We observed that a uniform flush reduced blockage, phlebitis, and exudation compared to a pulsed flush by visual observation. The histopathological examination found that the morphological changes in group A were more severe than in group B and C related to loss of venous endothelial cells, inflammatory cell infiltration, edema, epidermal and chondrocyte degeneration, except for the thrombosis on group B that was more serious than in group A, especially in the distal side of puncture points. The distal region of groups A and B had more inflammatory cell infiltration than the proximal region. Thrombosis was more severe in the distal region than in the proximal region in group B. CONCLUSIONS: The uniform flush produced less damage to the vascular endothelium and surrounding tissues and was superior to the pulsed flush. However, the uniform flush is prone to thrombosis.


Assuntos
Vasos Sanguíneos/patologia , Cateterismo Periférico/métodos , Animais , Cateterismo Periférico/efeitos adversos , Modelos Animais de Doenças , Orelha/irrigação sanguínea , Células Endoteliais , Endotélio Vascular/patologia , Endotélio Vascular/ultraestrutura , Masculino , Flebite/etiologia , Coelhos , Distribuição Aleatória , Fluxo Sanguíneo Regional
15.
Molecules ; 24(18)2019 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-31546796

RESUMO

Polymethylene-interrupted polyunsaturated fatty acids (PMI-PUFAs) are emerging functional lipids with proven antioxidant and anti-inflammatory effects. In this study, a typical PMI-PUFA, sciadonic acid (C20:3, 5c 11c 14c), was enriched in the kernel oil of Torreya fargesii (T. fargesii) by fractionation. Fractionated kernel oil of T. fargesii (containing 25% sciadonic acid) showed equal stability and similar radical scavenging ability compared with the non-fractionated oil. In anti-inflammatory tests, fractionated kernel oil was shown to inhibit the activity of phosphodiesterase (PDE-5, efficiency 80% at 133.7 µg/mL) and lipoxygenase-5 (LOX-5, efficiency 65% at 66.7 µg/mL) more effectively than the non-fractionated oil. This shows that increasing the amount of sciadonic acid can enhance the anti-inflammatory effect of the kernel oil. This research also indicates that fractionation is a feasible way to obtain sciadonic acid-rich functional oil with potential pharmacological effects.


Assuntos
Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Óleos Vegetais/química , Sementes/química , Taxaceae/química , Animais , Araquidonato 5-Lipoxigenase/metabolismo , Compostos de Bifenilo/química , Fracionamento Químico , Nucleotídeo Cíclico Fosfodiesterase do Tipo 5/metabolismo , Orelha/patologia , Edema/patologia , Ácidos Graxos/análise , Depuradores de Radicais Livres/análise , Camundongos , Picratos/química
17.
Anim Genet ; 50(6): 557-568, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31475748

RESUMO

Together with their sister subspecies Bos taurus, zebu cattle (Bos indicus) have contributed to important socioeconomic changes that have shaped modern civilizations. Zebu cattle were domesticated in the Indus Valley 8000 years before present (YBP). From the domestication site, they expanded to Africa, East Asia, southwestern Asia and Europe between 4000 and 1300 YBP, intercrossing with B. taurus to form clinal variations of zebu ancestry across the landmass of Afro-Eurasia. In the past 150 years, zebu cattle reached the Americas and Oceania, where they have contributed to the prosperity of emerging economies. The zebu genome is characterized by two mitochondrial haplogroups (I1 and I2), one Y chromosome haplogroup (Y3) and three major autosomal ancestral groups (Indian-Pakistani, African and Chinese). Phenotypically, zebu animals are recognized by their hump, large ears and excess skin. They are rustic, resilient to parasites and capable of bearing the hot and humid climates of the tropics. Many resources are available to study the zebu genome, including commercial arrays of SNP, reference assemblies and publicly available genotypes and whole-genome sequences. Nevertheless, many of these resources were initially developed to support research and subsidize industrial applications in B. taurus, and therefore they can produce bias in data analysis. The combination of genomics with precision agriculture holds great promise for the identification of genetic variants affecting economically important traits such as tick resistance and heat tolerance, which were naturally selected for millennia and played a major role in the evolution of B. indicus cattle.


Assuntos
Bovinos/genética , Bovinos/fisiologia , Animais , Evolução Biológica , Bovinos/anatomia & histologia , Resistência à Doença , Domesticação , Orelha/anatomia & histologia , Fertilidade , Variação Genética , Tamanho do Órgão , Pele/anatomia & histologia
18.
Orv Hetil ; 160(39): 1533-1541, 2019 Sep.
Artigo em Húngaro | MEDLINE | ID: mdl-31544493

RESUMO

Our health is highly determined by the diverse microbial community living within our body and upon our skin. Balance among the members of the commensal microbiota is essential for the preservation of health. New generation sequencing is a rapid, sensitive method for determining the whole microbiome without prior hypothesis and also gives information on the resistance and virulence status. Application of this method can help to identify the pathogens contributing to different diseases, and also the protective bacteria inhibiting their growth. Detecting the changes of the microbiome helps to identify new therapeutic targets and establish targeted antibiotic therapy. Broad-spectrum antibiotics also act against the beneficial members of the microbial flora, which may lead to the development of recurrent or chronic disease. Ear, nose and throat infections are the most common infective diseases in humans and the leading cause for antibiotic prescription worldwide. In recent years, many studies using molecular biology methods were performed examining the microbiome of healthy humans and in otorhinolaryngologic diseases. In the present work, the authors review the changes of the microbiological communities in the healthy state and in various pathologic states in the anatomic regions of the ear, nose and throat. Orv Hetil. 2019; 160(39): 1533-1541.


Assuntos
Bactérias/classificação , Orelha/microbiologia , Microbiota , Nariz/microbiologia , Otolaringologia , Otorrinolaringopatias/microbiologia , Faringe/microbiologia , Antibacterianos/uso terapêutico , Bactérias/genética , Bactérias/patogenicidade , Infecções Bacterianas/tratamento farmacológico , Infecções Bacterianas/microbiologia , Humanos , Otolaringologia/tendências , Otorrinolaringopatias/tratamento farmacológico , Otorrinolaringopatias/terapia , Análise de Sequência de DNA/métodos
19.
J Craniofac Surg ; 30(7): 2268-2270, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31503118

RESUMO

In this study, the authors present the challenges of replantation and management of 2 patients who have total scalp, partial forehead and ear avulsion. A 39-year-old male farmer was admitted to the emergency room with the total scalp, forehead, partial right eyebrow and upper eyelid amputation after his hair was catched by an agricultural machine. Anastomoses of 2 arteries and 2 veins in the occipital region were done first. Anastomosis of the right and left superficial temporal arteries and veins were performed end to end by turning the operating table without moving the head, followed by 1 vein anastomoses in the frontal region. Although anastomotic blood flow was observed by Doppler ultrasonography, there were changes suggesting necrosis in the frontal and both temporoparietal regions and later in the occipital region. Necrotic tissues began to be debrided tangentially from the 10th day. During serial debridement, it was observed that the necrotic tissue includes skin and connective tissue. At the end of the post-operative second month, surgical treatment was completed and 12 months after the surgery, the patient lives with the wig because of hair loss, but the patient is cosmetically satisfied. An 18-year-old female gatherer was admitted to the emergency room with the total scalp, both eyebrows, right upper eyelid and partial right ear avulsion, after her hair was catched by an agricultural machine. Totally, anastomoses of 4 arteries and 3 veins were performed. On the 10th post-operative day, necrotic skin findings appeared and serial debridement were performed and it was revealed that aponeurosis was fully intact. The defect areas of skin and connective tissue were repaired with STSG. Intact partial temporal scalp tissue was present. Scalp replantation should always be considered as the first choice, according to the similar tissue principle of tissue repair in plastic surgery if there is no contraindication. Additionally, performing multiple arterial and vein anastomoses, evaluating the patient position in the post-operative period for anastomosis and hemodynamic follow-up, avoiding aggressive debridement and early reconstruction may give the patient the chance of skin grafting which is the basis of the reconstructive ladder.


Assuntos
Orelha/cirurgia , Testa/cirurgia , Procedimentos Cirúrgicos Reconstrutivos , Couro Cabeludo/cirurgia , Adolescente , Adulto , Alopecia , Anastomose Cirúrgica , Desbridamento , Orelha/irrigação sanguínea , Feminino , Testa/irrigação sanguínea , Cabelo , Humanos , Masculino , Microcirurgia , Necrose/cirurgia , Couro Cabeludo/irrigação sanguínea
20.
J Int Adv Otol ; 15(2): 204-208, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31418720

RESUMO

The aim of this report is to provide international recommendations for functional ear reconstruction in patients with microtia and aural atresia. All patients with microtia and external auditory atresia should be seen in the setting of a multidisciplinary team and agreed treatment outcomes should be measured, so that techniques, approaches, and results can be compared. The methods are expert opinion from the members of the International Microtia and Atresia Workgroup (IMAW). The consensus recommendations reported herein take into account the variability in practice patterns present among experts in the field; the degree of consensus was quantified by presenting the percentage of above authors who agree or partially agree with each statement. Recommendations include the definition and classification of microtia/atresia, treatment of microtia, treatment of congenital aural atresia, flowchart of functional ear reconstruction, and future research directions. Patients with microtia and aural atresia can be guided by the consensus recommendations provided herein.


Assuntos
Anormalidades Congênitas/cirurgia , Microtia Congênita/cirurgia , Orelha Externa/cirurgia , Orelha Média/cirurgia , Orelha/anormalidades , Criança , Pré-Escolar , Constrição Patológica/cirurgia , Orelha/cirurgia , Humanos , Procedimentos Cirúrgicos Reconstrutivos/métodos , Resultado do Tratamento
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