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1.
Biomed Pharmacother ; 137: 111341, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33561646

RESUMO

Mycobacterium bovis (M. bovis) is a member of mycobacterium tuberculosis complex (MTBC), and a causative agent of chronic respiratory disease in a wide range of hosts. Bacillus Calmette-Guerin (BCG) vaccine is mostly used for the prevention of childhood tuberculosis. Further substantial implications are required for the development and evaluation of new tuberculosis (TB) vaccines as well as improving the role of BCG in TB control strategies. In this study, we prepared PLGA nanoparticles encapsulated with argF antigen (argF-NPs). We hypothesized, that argF nanoparticles mediate immune responses of BCG vaccine in mice models of M. bovis infection. We observed that mice vaccinated with argF-NPs exhibited a significant increase in secretory IFN-γ, CD4+ T cells response and mucosal secretory IgA against M. bovis infection. In addition, a marked increase was observed in the level of secretory IL-1ß, TNF-α and IL-10 both in vitro and in vivo upon argF-NPs vaccination. Furthermore, argF-NPs vaccination resulted in a significant reduction in the inflammatory lesions in the lung's tissues, minimized the losses in total body weight and reduced M. bovis burden in infected mice. Our results indicate that BCG prime-boost strategy might be a promising measure for the prevention against M. bovis infection by induction of CD4+ T cells responses and mucosal antibodies.


Assuntos
Vacina BCG/administração & dosagem , Vacina BCG/imunologia , Mycobacterium bovis , Nanopartículas/administração & dosagem , Ornitina Carbamoiltransferase/imunologia , Copolímero de Ácido Poliláctico e Ácido Poliglicólico/imunologia , Tuberculose Bovina/prevenção & controle , Administração Intranasal , Animais , Formação de Anticorpos/efeitos dos fármacos , Peso Corporal/efeitos dos fármacos , Linfócitos T CD4-Positivos/metabolismo , Bovinos , Linhagem Celular , Modelos Animais de Doenças , Feminino , Imunoglobulina A Secretora/metabolismo , Imunoglobulina G/sangue , Interferon gama/metabolismo , Interleucina-10/sangue , Interleucina-1beta/sangue , Pulmão/metabolismo , Pulmão/microbiologia , Pulmão/patologia , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Camundongos Endogâmicos BALB C , Mycobacterium bovis/crescimento & desenvolvimento , Mycobacterium bovis/patogenicidade , Nanopartículas/química , Ornitina Carbamoiltransferase/administração & dosagem , Ornitina Carbamoiltransferase/química , Copolímero de Ácido Poliláctico e Ácido Poliglicólico/administração & dosagem , Copolímero de Ácido Poliláctico e Ácido Poliglicólico/química , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/química , Proteínas Recombinantes/imunologia , Baço/microbiologia , Baço/patologia , Fator de Necrose Tumoral alfa/sangue
2.
J Microbiol Immunol Infect ; 53(2): 234-239, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29934035

RESUMO

BACKGROUND: Streptococcus suis (SS) is a major swine pathogen and a serious zoonotic pathogen causing septicemia and meningitis in piglets and humans. Using an immunoproteomic approach, we previously brought evidence that ornithine carbamoytransferase (OCT) may represent a vaccine candidate to protect against S. suis biofilm-related and acute infections. METHOD: In this study, the gene encoding OCT was cloned into the expression vector pET-28a and the recombinant protein was expressed in Escherichia coli BL21. The immunogenicity and protective efficacy of the SS OCT was further investigated in a mouse model. RESULTS: The protein was found to be expressed in vivo and elicited high antibody titers following SS infections in mice. An animal challenge experiment with SS showed that 62.5% of mice immunized with the OCT protein were protected. Using an in vitro competitive adherence inhibition assay of adherence, evidence was obtained that OCT could significantly reduce the number of SS cells adhered to porcine kidney PK-15 cells. The bacterial levels recovered in mice of the OCT immunized group were significantly decreased in some organs, compared with the control group. CONCLUSION: In summary, our results suggest that the recombinant SS OCT protein, which is involved in bacterial adherence, may efficiently stimulate an immune response conferring protection against SS infections. It may therefore be considered as a potential vaccine candidate, although further studies are necessary to evaluate their use in swine.


Assuntos
Aderência Bacteriana/fisiologia , Ornitina Carbamoiltransferase/imunologia , Ornitina Carbamoiltransferase/isolamento & purificação , Infecções Estreptocócicas/imunologia , Streptococcus suis/enzimologia , Animais , Anticorpos Antibacterianos/sangue , Antígenos de Bactérias/genética , Antígenos de Bactérias/imunologia , Proteínas de Bactérias/genética , Vacinas Bacterianas/imunologia , Biofilmes , Modelos Animais de Doenças , Escherichia coli/genética , Imunização , Camundongos , Ornitina/metabolismo , Ornitina Carbamoiltransferase/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Infecções Estreptocócicas/microbiologia , Infecções Estreptocócicas/prevenção & controle , Streptococcus suis/genética , Streptococcus suis/imunologia
3.
Cell Immunol ; 346: 103997, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31703913

RESUMO

An ongoing concern of in vivo gene therapy is adaptive immune responses against the protein product of a transgene, particularly for recessive diseases in which antigens are not presented to lymphocytes during central tolerance induction. Here we show that Toll-like receptor 9 (TLR9) signaling activates T cells against an epitope tagged mitochondria-targeted ornithine transcarbamylase (OTC) following the administration of a systemic adeno-associated virus (AAV) vector. Using a transgenic mouse model system, we demonstrate that TLR9 signaling extrinsic to T cells induces a robust cytotoxic T-cell response against the transgene and results in transgene expression loss. Overall, our results suggest that inflammation mediated by TLR9 signaling and the presence of high affinity transgene-specific T cells is important for the development of adaptive immune responses to transgene products following AAV gene therapy.


Assuntos
Dependovirus/imunologia , Linfócitos T Citotóxicos/imunologia , Receptor Toll-Like 9/genética , Receptor Toll-Like 9/imunologia , Imunidade Adaptativa/imunologia , Animais , Proteínas de Ligação a DNA/genética , Dependovirus/genética , Terapia Genética , Vetores Genéticos/genética , Inflamação/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Mitocôndrias/imunologia , Ornitina Carbamoiltransferase/imunologia , Transdução de Sinais/imunologia
4.
Fish Shellfish Immunol ; 41(2): 250-9, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25218275

RESUMO

Streptococcus agalactiae is a Gram-positive bacterium and a severe aquaculture pathogen that can infect a wide range of warmwater fish species. The outer-surface proteins in bacterial pathogens play an important role in pathogenesis. We evaluated the immunogenicity of two of the identified surface proteins namely phosphoglycerate kinase (PGK) and ornithine carbamoyl-transferase (OCT). PGK and OCT were over-expressed and purified from Escherichia coli and used as the subunit vaccines in tilapia. Tilapia immunized with the S. agalactiae modified bacteria vaccine (whole cell preparations with recombinant PGK and OCT proteins) individually were tested for the efficacy. OCT and PGK combined with WC had a higher survival rate. A high-level protection and significant specific antibody responses against S. agalactiae challenge was observed upon the vaccinated tilapia with the purified PGK protein and S. agalactiae whole cells. The specific antibody titer against S. agalactiae antigen suggested that increased antibody titers were correlated with post-challenge survival rate. Il-1ß expression profile was higher in PGK + WC-treated group. Tnf-α expression in the PGK + WC group was significantly increased. Taken together, our results suggested the combinations of recombinant protein and whole cell may elicit immune responses that reach greater protection than that of individual S. agalactiae components.


Assuntos
Vacinas Bacterianas/farmacologia , Ciclídeos , Doenças dos Peixes/imunologia , Doenças dos Peixes/microbiologia , Fosfoglicerato Quinase/imunologia , Infecções Estreptocócicas/veterinária , Streptococcus agalactiae/imunologia , Análise de Variância , Animais , Anticorpos Antibacterianos/imunologia , Vacinas Bacterianas/imunologia , Western Blotting , Primers do DNA/genética , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Escherichia coli , Doenças dos Peixes/prevenção & controle , Interleucina-1beta/imunologia , Ornitina Carbamoiltransferase/imunologia , Reação em Cadeia da Polimerase em Tempo Real , Infecções Estreptocócicas/imunologia , Infecções Estreptocócicas/prevenção & controle , Fator de Necrose Tumoral alfa/imunologia
5.
Vaccine ; 29(51): 9529-37, 2011 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-22001876

RESUMO

Giardia lamblia is a leading protozoan cause of diarrheal disease worldwide, yet preventive medical strategies are not available. A crude veterinary vaccine has been licensed for cats and dogs, but no defined human vaccine is available. We tested the vaccine potential of three conserved antigens previously identified in human and murine giardiasis, α1-giardin, α-enolase, and ornithine carbamoyl transferase, in a murine model of G. lamblia infection. Live recombinant attenuated Salmonella enterica Serovar Typhimurium vaccine strains were constructed that stably expressed each antigen, maintained colonization capacity, and sustained total attenuation in the host. Oral administration of the vaccine strains induced antigen-specific serum IgG, particularly IgG(2A), and mucosal IgA for α1-giardin and α-enolase, but not for ornithine carbamoyl transferase. Immunization with the α1-giardin vaccine induced significant protection against subsequent G. lamblia challenge, which was further enhanced by boosting with cholera toxin or sublingual α1-giardin administration. The α-enolase vaccine afforded no protection. Analysis of α1-giardin from divergent assemblage A and B isolates of G. lamblia revealed >97% amino acid sequence conservation and immunological cross-reactivity, further supporting the potential utility of this antigen in vaccine development. Together. These results indicate that α1-giardin is a suitable candidate antigen for a vaccine against giardiasis.


Assuntos
Proteínas do Citoesqueleto/imunologia , Giardia lamblia/imunologia , Giardíase/prevenção & controle , Proteínas de Protozoários/imunologia , Vacinas Protozoárias/imunologia , Administração Oral , Animais , Anticorpos Antiprotozoários/biossíntese , Antígenos de Protozoários/imunologia , Toxina da Cólera/imunologia , Proteínas do Citoesqueleto/administração & dosagem , Giardíase/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Ornitina Carbamoiltransferase/administração & dosagem , Ornitina Carbamoiltransferase/imunologia , Fosfopiruvato Hidratase/administração & dosagem , Fosfopiruvato Hidratase/imunologia , Proteínas de Protozoários/administração & dosagem , Vacinas Protozoárias/administração & dosagem , Salmonella typhimurium/genética , Vacinas Sintéticas/administração & dosagem , Vacinas Sintéticas/imunologia
6.
Infect Immun ; 78(9): 3957-68, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20605988

RESUMO

Clostridium perfringens is a medically important clostridial pathogen and an etiological agent causing several diseases in humans and animals. C. perfringens and its toxins have been listed as potential biological and toxin warfare (BTW) agents; thus, efforts to develop strategies for detection and protection are warranted. Forty-eight extracellular proteins of C. perfringens type A and type C strains have been identified here using a 2-dimensional gel electrophoresis-mass spectrometry (2-DE-MS) technique. The SagA protein, the DnaK-type molecular chaperone hsp70, endo-beta-N-acetylglucosaminidase, and hypothetical protein CPF_0656 were among the most abundant proteins secreted by C. perfringens ATCC 13124. The antigenic component of the exoproteome of this strain has also been identified. Most of the extracellular proteins were predicted to be involved in carbohydrate transport and metabolism (16%) or cell envelope biogenesis or to be outer surface protein constituents (13%). More than 50% of the proteins were predictably secreted by either classical or nonclassical pathways. LipoP and TMHMM indicated that nine proteins were extracytoplasmic but cell associated. Immunization with recombinant ornithine carbamoyltransferase (cOTC) clearly resulted in protection against a direct challenge with C. perfringens organisms. A significant rise in IgG titers in response to recombinant cOTC was observed in mice, and IgG2a titers predominated over IgG1 titers (IgG2a/IgG1 ratio, 2). The proliferation of spleen lymphocytes in cOTC-immunized animals suggested a cellular immune response. There were significant increases in the levels of gamma interferon (IFN-gamma) and interleukin 2 (IL-2), suggesting a Th1 type immune response.


Assuntos
Proteínas de Bactérias/análise , Clostridium perfringens/química , Proteômica/métodos , Animais , Proteínas de Bactérias/imunologia , Clostridium perfringens/classificação , Clostridium perfringens/patogenicidade , Eletroforese em Gel Bidimensional , Feminino , Camundongos , Camundongos Endogâmicos BALB C , Ornitina Carbamoiltransferase/imunologia , Virulência
7.
Clin Vaccine Immunol ; 15(4): 638-43, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18305107

RESUMO

Although the antigen detection assay has the potential to discriminate active tuberculosis from latent infection, development of such a test for the accurate diagnosis of this serious disease has only recently become a matter of interest. Here we present evidence that a Mycobacterium tuberculosis protein (ornithine carboamyltransferase, coded for by MT_1694; Rv1656 [argF]) is an interesting candidate molecule for this test development. The protein was initially discovered by mass spectroscopy in urine of patients with pulmonary tuberculosis and shown by Western blot analysis to be present in M. tuberculosis crude cell extract as well as in the culture supernatant ("secreted" protein). In addition, a recombinant ornithine carboamyltransferase (rMT1694) produced in Escherichia coli was recognized by immunoglobulin G (IgG) antibodies from patients with active tuberculosis but not by IgG from uninfected healthy subjects. Moreover, rMT1694 was strongly recognized by peripheral blood mononuclear cells from both healthy tuberculin purified protein derivative (PPD)-positive individuals and patients with pulmonary tuberculosis. More importantly, a capture enzyme-linked immunosorbent assay formatted with rabbit IgG antibodies specific to rMT1694 was able to identify the presence of this antigen in urine samples from 6 of 16 patients with pulmonary tuberculosis and in none of 16 urine samples collected from healthy PPD(+) controls. These results indicate that an improved antigen detection assay based on M. tuberculosis ornithine carboamyltransferase may represent an important new strategy for the development of a specific and accurate diagnostic test for tuberculosis.


Assuntos
Mycobacterium tuberculosis/enzimologia , Ornitina Carbamoiltransferase/urina , Tuberculose Pulmonar/urina , Animais , Especificidade de Anticorpos , Antígenos de Bactérias/urina , Clonagem Molecular , Ensaio de Imunoadsorção Enzimática/métodos , Humanos , Imunoglobulina G/imunologia , Mycobacterium tuberculosis/imunologia , Neutrófilos/imunologia , Ornitina Carbamoiltransferase/genética , Ornitina Carbamoiltransferase/imunologia , Coelhos , Proteínas Recombinantes/imunologia , Tuberculose Pulmonar/sangue , Tuberculose Pulmonar/imunologia , Tuberculose Pulmonar/microbiologia
8.
Clin Chim Acta ; 368(1-2): 125-30, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16445902

RESUMO

BACKGROUND: We developed a new enzyme-linked immunosorbent assay (ELISA) for ornithine carbamoyltransferase (OCT) and evaluated its usefulness. METHODS: Recombinant human OCT expressed in E. coli was used as an antigen to obtain the monoclonal antibodies for this assay. RESULTS: The reactivity of the antibodies to native OCT as well as recombinant OCT was enhanced at alkaline pH (8.5-10), and the assay's sensitivity was markedly improved. The antibodies react identically with native and recombinant OCT at pH 9.4. The dilution test showed a good linearity between dilution ratios and the concentrations. Different concentrations of OCT added were recovered on average at 90.4%. There was a good correlation between OCT protein levels in the ELISA and OCT enzyme activities (r=0.987, p<0.0001). A significant difference in the serum level of OCT was observed between chronic hepatitis patients (110.7+/-80 ng/ml) and healthy subjects (34.4+/-20.7 ng/ml) (p<0.0001). The serum levels of OCT between sexes differed significantly in the healthy subjects (p<0.0001). CONCLUSIONS: Our newly established ELISA for OCT using monoclonal antibodies is sensitive enough for clinical application.


Assuntos
Álcalis , Ensaio de Imunoadsorção Enzimática/métodos , Ornitina Carbamoiltransferase/sangue , Ornitina Carbamoiltransferase/imunologia , Alanina Transaminase/sangue , Cromatografia em Gel , Expressão Gênica , Saúde , Hepatite Crônica/sangue , Humanos , Concentração de Íons de Hidrogênio , Ornitina Carbamoiltransferase/genética , Ornitina Carbamoiltransferase/isolamento & purificação , Sensibilidade e Especificidade
9.
Infect Immun ; 70(3): 1254-9, 2002 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11854208

RESUMO

To identify the major outer surface proteins of Streptococcus agalactiae (group B streptococcus), a proteomic analysis was undertaken. An extract of the outer surface proteins was separated by two-dimensional electrophoresis. The visualized spots were identified through a combination of peptide sequencing and reverse genetic methodologies. Of the 30 major spots identified as S. agalactiae specific, 27 have been identified. Six of these proteins, previously unidentified in S. agalactiae, were sequenced and cloned. These were ornithine carbamoyltransferase, phosphoglycerate kinase, nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase, purine nucleoside phosphorylase, enolase, and glucose-6-phosphate isomerase. Using a gram-positive expression system, we have overexpressed two of these proteins in an in vitro system. These recombinant, purified proteins were used to raise antisera. The identification of these proteins as residing on the outer surface was confirmed by the ability of the antisera to react against whole, live bacteria. Further, in a neonatal-animal model system, we demonstrate that some of these sera are protective against lethal doses of bacteria. These studies demonstrate the successful application of proteomics as a technique for identifying vaccine candidates.


Assuntos
Proteínas da Membrana Bacteriana Externa/isolamento & purificação , Streptococcus agalactiae/química , Sequência de Aminoácidos , Animais , Animais Recém-Nascidos , Anticorpos Antibacterianos/imunologia , Proteínas da Membrana Bacteriana Externa/imunologia , Eletroforese em Gel Bidimensional , Imunização Passiva , Camundongos , Dados de Sequência Molecular , Ornitina Carbamoiltransferase/imunologia , Fosfoglicerato Quinase/imunologia , Proteoma , Análise de Sequência de Proteína , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Infecções Estreptocócicas/etiologia , Infecções Estreptocócicas/prevenção & controle , Streptococcus agalactiae/imunologia
10.
Cancer Res ; 54(6): 1536-41, 1994 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-7511043

RESUMO

Overexpression of P-glycoprotein, the plasma membrane protein product of the MDR1 gene, is a major determinant in the development of resistance to a large number of cancer chemotherapeutic agents. A battery of antibodies, including the MDR1 gene-specific monoclonal antibody (mAb) C494, is used to evaluate human tissues in clinical multidrug resistance surveillance and modulation trials. In rat liver fractions, we report that mAb C494 strongly cross-reacted with a nonmembranous M(r) approximately 130,000 protein, comigrating with core-glycosylated human MDR1 on 7% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. By immunoblotting and microsequence analysis, this protein was identified as pyruvate carboxylase (PC), an abundant mitochondrial enzyme. A search of the National Center for Biotechnology Information data base, using the epitope-specific sequence of mAb C494, revealed that PC (mouse) contains four of the five most reactive amino acids (TLEG), located near the COOH-terminal end of PC at positions 1167-1170. mAb C494 specifically reacted with PC purified from bovine liver; immunoreactivity was completely abolished by preincubating mAb C494 in the presence of excess synthetic C494 epitope-specific peptide. Furthermore, in cryosections of human skeletal muscle, a tissue known not to express P-glycoprotein, peptide-displaceable immunohistochemical staining with mAb C494 showed a distinct mitochondrial pattern specific to type 1 fibers. Variable immunostaining results were obtained with formaldehyde-fixed, paraffin-embedded muscle and isolated liver mitochondrial preparations. In summary, mAb C494 cross-reacted strongly with rat, bovine, and human PC. Caution is warranted in interpretation of immunoblots and immunohistochemical sections with this putative MDR1 gene-specific mAb.


Assuntos
Anticorpos Monoclonais/genética , Anticorpos Monoclonais/imunologia , Piruvato Carboxilase/imunologia , Membro 1 da Subfamília B de Cassetes de Ligação de ATP , Sequência de Aminoácidos , Animais , Especificidade de Anticorpos , Proteínas de Transporte/análise , Proteínas de Transporte/imunologia , Bovinos , Membrana Celular/química , Membrana Celular/enzimologia , Reações Cruzadas , Resistência a Medicamentos/genética , Epitopos/imunologia , Humanos , Immunoblotting , Imuno-Histoquímica , Fígado/enzimologia , Glicoproteínas de Membrana/análise , Glicoproteínas de Membrana/imunologia , Mitocôndrias Hepáticas/enzimologia , Dados de Sequência Molecular , Músculos/química , Ornitina Carbamoiltransferase/imunologia , Piruvato Carboxilase/análise , Ratos , Ratos Sprague-Dawley , ATPase Trocadora de Sódio-Potássio/imunologia , Frações Subcelulares/química , Frações Subcelulares/enzimologia
11.
Plant Physiol ; 92: 1205-10, 1990.
Artigo em Inglês | MEDLINE | ID: mdl-11537995

RESUMO

Pea (Pisum sativum L.) ornithine transcarbamylase (OTC) antisera were used to investigate the immunological relatedness of several plant and animal OTC enzymes. The antisera immunoprecipitated OTC activity in all monocot and dicot species tested, and sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of immunoprecipitated protein revealed monomeric proteins ranging from 35,200 to 36,800 daltons in size. Pea OTC antisera did not recognize mammalian OTC protein. OTC activity and protein levels detected on sodium dodecyl sulfate polyacrylamide gel electrophoresis immunoblots from homogenates of green leaf, etiolated epicotyl and cotyledon, and root tissues of pea were poorly correlated. This might result from differences in amounts of enzymatically active OTC protein in the homogenates. Alternatively, the antisera may fail to recognize different isozyme forms of OTC, which have been reported for some plant species. A putative cytosolic precursor OTC (pOTC) polypeptide exhibiting and Mr = 39,500 to 40,000 daltons was immunoprecipitated from in vitro translation mixtures of total pea leaf poly(A)+ RNA. The size of the pOTC polypeptide, as compared with mature OTC monomer (36,000 daltons), suggests that a 4 kilodalton N-terminal leader sequence, like that responsible for mitochondrial targeting of the mammalian enzyme, may be involved in organellar import of the plant enzyme.


Assuntos
Precursores Enzimáticos/isolamento & purificação , Ornitina Carbamoiltransferase/imunologia , Proteínas de Vegetais Comestíveis/imunologia , Plantas/enzimologia , Animais , Avena/enzimologia , Avena/genética , Avena/imunologia , Precursores Enzimáticos/análise , Precursores Enzimáticos/imunologia , Fabaceae/enzimologia , Fabaceae/genética , Fabaceae/imunologia , Humanos , Soros Imunes , Lycopersicon esculentum/enzimologia , Lycopersicon esculentum/genética , Lycopersicon esculentum/imunologia , Ornitina Carbamoiltransferase/análise , Ervilhas/enzimologia , Ervilhas/genética , Ervilhas/imunologia , Proteínas de Vegetais Comestíveis/análise , Proteínas de Vegetais Comestíveis/isolamento & purificação , Plantas/genética , Plantas/imunologia , Plantas Medicinais , Plantas Tóxicas , RNA Mensageiro/imunologia , RNA Mensageiro/isolamento & purificação , Ratos , Tabaco/enzimologia , Tabaco/genética , Tabaco/imunologia , Zea mays/enzimologia , Zea mays/genética , Zea mays/imunologia
12.
J Gen Microbiol ; 135(9): 2453-64, 1989 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-2516870

RESUMO

An immunological approach was used for the study of ornithine carbamoyltransferase (OTCase) evolution in bacteria. Antisera were prepared against the anabolic and catabolic OTCases of Pseudomonas aeruginosa and Aeromonas formicans as well as against OTCase and putrescine carbamoyltransferases from Streptococcus faecalis; these antisera were then tested against the unpurified OTCases, either anabolic or catabolic, of 34 bacterial strains. Extensive cross-reactions were observed between the antisera to catabolic OTCases from P. aeruginosa, A. formicans and S. faecalis and the catabolic enzymes from other species or genera. These antisera cross-reacted also with the anabolic OTCases of strains of the Enterobacteriaceae but not with the anabolic OTCases of the same species or of other species or genera. The cross-reaction measured between the antisera against P. aeruginosa anabolic OTCase and the anabolic OTCases of other Pseudomonas were largely in agreement with the phylogenic subdivision of Pseudomonas proposed by N. J. Palleroni. The correlation was also significantly higher with the anabolic enzyme of an archaeobacterium, Methanobacterium thermoaceticum, than with the catabolic or anabolic OTCases from other genera in the eubacterial line. The antiserum raised against A. formicans anabolic OTCase was quite specific for its antigen and appeared to be raised against the heaviest of the various oligomeric structures of the enzyme.


Assuntos
Bactérias/enzimologia , Evolução Biológica , Ornitina Carbamoiltransferase/imunologia , Aeromonas/enzimologia , Sequência de Aminoácidos , Especificidade de Anticorpos/fisiologia , Antígenos de Bactérias/imunologia , Bactérias/imunologia , Cromatografia em Gel , Soros Imunes/imunologia , Dados de Sequência Molecular , Pseudomonas aeruginosa/enzimologia
13.
Tohoku J Exp Med ; 152(1): 15-21, 1987 May.
Artigo em Inglês | MEDLINE | ID: mdl-3617058

RESUMO

Antigen contents of ornithine transcarbamylase (OTC) protein in the liver of 5 patients with OTC deficiency and mutant mice were studied by ELISA. OTC activities in the liver tissues of 2 patients were 13.1% and 5.2% of the controls, respectively. The 2 patients' antigen contents of OTC protein were decreased in parallel with the enzyme activities. OTC antigen contents were not detected in the liver tissues of remaining 3 patients who had the complete type of OTC deficiency. Enzyme activities, kinetic properties and antigen contents of the OTC deficient mutant mice were the same as the results reported previously (Briand et al. 1982). The ELISA method for the assay of OTC antigen contents used in this study is more sensitive as compared with the radial immunodiffusion technique.


Assuntos
Fígado/enzimologia , Doença da Deficiência de Ornitina Carbomoiltransferase , Animais , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Concentração de Íons de Hidrogênio , Cinética , Masculino , Camundongos , Ornitina Carbamoiltransferase/imunologia , Ornitina Carbamoiltransferase/metabolismo
14.
J Bacteriol ; 161(2): 714-9, 1985 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-3968036

RESUMO

Purified catabolic ornithine carbamoyltransferase of Pseudomonas putida and anabolic ornithine carbamoyltransferase (argF product) of Escherichia coli K-12 were used to prepare antisera. The two specific antisera gave heterologous cross-reactions of various intensities with bacterial catabolic ornithine carbamoyltransferases formed by Pseudomonas and representative organisms of other bacterial genera. The immunological cross-reactivity observed only between the catabolic ornithine carbamoyltransferases and the anabolic enzymes of enterobacteria suggests that these proteins share some structural similarities. Indeed, the amino acid composition of the anabolic ornithine carbamoyltransferase of E. coli K-12 (argF and argI products) closely resembles the amino acid compositions of the catabolic enzymes of Pseudomonas putida, Aeromonas formicans, Streptococcus faecalis, and Bacillus licheniformis. Comparison of the N-terminal amino acid sequence of the E. coli anabolic ornithine carbamoyltransferase with that of the A. formicans and Pseudomonas putida catabolic enzymes shows, respectively, 45 and 28% identity between the compared positions; the A. formicans sequence reveals 53% identity with the Pseudomonas putida sequence. These results favor the conclusion that anabolic ornithine carbamoyltransferases of enterobacteria and catabolic ornithine carbamoyltransferases derive from a common ancestral gene.


Assuntos
Enterobacteriaceae/enzimologia , Ornitina Carbamoiltransferase/análise , Sequência de Aminoácidos , Aminoácidos/análise , Especificidade de Anticorpos , Ornitina Carbamoiltransferase/imunologia
16.
Biochim Biophys Acta ; 704(1): 100-6, 1982 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-7093283

RESUMO

Enzymatic activities and kinetics of liver ornithine transcarbamylase (carbamoylphosphate:L-ornithine carbamoyltransferase) were studied in 16 human males with ornithine transcarbamylase mutations. In the same liver fragments, cross-reactive material was measured with specific anti-ornithine transcarbamylase antibody. These studies allowed us to describe five groups of mutations. Two of them were similar on the basis of their enzymatic properties and cross-reactive material amounts to two mouse ornithine transcarbamylase mutations:sparse-fur (spf) and sparse-fur with abnormal skin and hair (spf-ash).


Assuntos
Doença da Deficiência de Ornitina Carbomoiltransferase , Amônia/sangue , Animais , Reações Cruzadas , Humanos , Cinética , Fígado/enzimologia , Masculino , Camundongos , Mutação , Ornitina Carbamoiltransferase/genética , Ornitina Carbamoiltransferase/imunologia
17.
J Bacteriol ; 129(3): 1387-96, 1977 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-14923

RESUMO

Ornithine transcarbamylase (OTCase) was purified to hemogeneity from a derepressed strain of Salmonella typhimurium. The optimal pH for enzyme activity is 8.0. The molecular weight of the enzyme was calculated to be 116,000, based on measurements of the sedimentation coefficient by sucrose gradient ultracentrifugation and the Stokes radius by gel filtration. Polyacrylamide gel electrophoresis of cross-linked OTCase in the presence of sodium dodecyl sulfate showed that the enzyme is composed of three identical subunits. The molecular weight of the monomer was determined to be 39,000. Steady-state kinetics indicate that the reaction mechanism is sequential. The limiting Michealis constants for carbamylphosphate and ornithine were determined to be 0.06 and 0.2 mM, respectively. The dissociation constant for carbamylphosphate was 0.02 mM. Product and dead-end inhibition patterns are consistent with an ordered Bi Bi mechanism, in which carbamylphosphate is the first substrate added and phosphate is the last product released. OTCase activity was inhibited by arginine, but relatively high concentrations were required for significant inhibition. The inhibition by arginine might be physiologically significant in the regulation of carbamlphosphate utilization; a single carbamylphosphate synthetase is responsible for the synthesis of carbamylphosphate for both arginine and pyrimidines in S. typhimurium and the inhibition by argine might serve to divert carbamlphosphate to the synthesis of pyrimidines when arginine is present at high concentrations. The crossreaction of OTCases from different microorganisms with purified antibodies raised against the homogeneous OTCase from S. typhimurium was investigated. The results of immunotitration and immunodiffusion experiments revealed a high degree of identity between the enzymes form S. typhimurium and Esherichia coli B and W. In these three cases, a single gen (argl) encodes OTCase. Wild-type E. coli K-12 and strain 3000 X 111, which carry two OTCase genes (argI, argF), also revealed similar cross-reactivity, supporting the hypothesis that argF is the product of a relatively recent duplication. The activity of OTCase from Bacillus subtilis was partially inhibited by antibodies against the enzyme from S. typhimurium, indicating unusual conservation of primary structure among widely different taxonomic groups. OTCase from Saccharomyces cerevisiae, whose molecular weight and primary structure are similar to those of the enzyme from S. typhimurium, was without detectable cross-reactivity.


Assuntos
Ornitina Carbamoiltransferase/metabolismo , Salmonella typhimurium/enzimologia , Arginina/farmacologia , Citrulina/farmacologia , Reações Cruzadas , Concentração de Íons de Hidrogênio , Cinética , Conformação Molecular , Peso Molecular , Ornitina Carbamoiltransferase/imunologia , Ornitina Carbamoiltransferase/isolamento & purificação
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