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1.
Anticancer Res ; 39(10): 5605-5610, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31570456

RESUMO

BACKGROUND/AIM: We analyzed the process of healing at osteotomy sites and timing of achievement of full weight-bearing in sarcoma patients who underwent hemicortical or intercalary reconstruction using the extracorporeal irradiated autologous bone graft technique. PATIENTS AND METHODS: We studied 10 patients who had undergone tumor resection and reconstruction with hemicortical extracorporeal irradiated autologous bone graft at mid-shaft femur or tibia. The control group consisted of 30 patients who received the reconstruction using intercalary bone graft. RESULTS: Full weight-bearing was achieved in all 10 patients at a median time of 4.8 months. Function was excellent in all patients. When comparing the clinical outcome among the patients who received intercalary and hemicortical grafts, the duration to full weight-bearing achievement in patients who received hemicortical graft was shorter than that in those with intercalary graft. CONCLUSION: Early full weight-bearing may be achieved in patients undergoing hemicortical resection and reconstruction using extracorporeal irradiated autologous bone graft.


Assuntos
Neoplasias Ósseas/patologia , Neoplasias Ósseas/cirurgia , Sarcoma/patologia , Sarcoma/cirurgia , Neoplasias de Tecidos Moles/patologia , Neoplasias de Tecidos Moles/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , Transplante Ósseo/métodos , Osso e Ossos/patologia , Osso e Ossos/cirurgia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Osteotomia , Transplante Autólogo/métodos , Resultado do Tratamento
2.
J Forensic Leg Med ; 67: 24-27, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31377682

RESUMO

BACKGROUND: A mass grave is any site that containing two or more associated corpses, at random or on purpose placed, of people who have died as a result of extra-judicial or random executions, not including those people who have died from armed confrontations or known major catastrophes. CASE PRESENTATION: The purpose of this paper is to explain how to reconstruct a biological profile of decomposed or skeletonized bodies and clarify the efforts done by the Libyan scientist after 2011 revolution and to set a reference for other researcher. The alleged location of the grave, as well as the alleged number and identities of the persons buried in the grave were obtained exclusively from witnesses' and relatives' testimonies. CONCLUSION: As the testimonies said, the grave was located at the alleged location and seven skeletons were exhumed. Also, the osteological and DNA study made investigators to identify the exhumed skeletons. And the dental analysis support the identification of a seven man alleged to have been buried in the grave, 7 victims were discovered.


Assuntos
Osso e Ossos/patologia , Impressões Digitais de DNA , Exumação , Adulto , Determinação da Idade pelo Esqueleto , Sepultamento , DNA/isolamento & purificação , Antropologia Forense , Odontologia Legal , Humanos , Líbia , Masculino , Repetições de Microssatélites , Determinação do Sexo pelo Esqueleto , Dente/química , Guerra
3.
Medicine (Baltimore) ; 98(27): e16045, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31277098

RESUMO

RATIONALE: Synovitis, acne, pustulosis, hyperostosis, and osteitis (SAPHO) syndrome is a rare auto-inflammatory disease with no standardized treatment. Systemic corticosteroids are only transiently effective, but long-term use would bring complications and would not bring long-term remission. Bone scintigraphy is a first-line method for systematic evaluation of osteoarticular lesions but seems to show an "imprinting" pattern. PATIENT CONCERNS: A 31-year-old female patient presented significant palmoplantar pustulosis and nail lesion as well as typical tracer accumulation feature on bone scintigraphy with normal hypersensitivity C-reactive protein and erythrocyte sedimentation rate, but an elevated serum immunoglobulin E level. DIAGNOSIS: The diagnosis was made by dermatological manifestations and classical sign in bone scintigraphy in accordance with the diagnostic criteria proposed in 1988. INTERVENTIONS: Methylprednisolone was given with a primary dose of 40 mg/day for 1 week followed with a subsequent 20 mg/day oral prednisone for another 1 week and then reduced in a rate of 5 mg/week until the eventual cessation. OUTCOMES: Long-term remarkable remission on clinical manifestations, MRI performance, and quantitative analysis of bone scintigraphy was achieved. LESSONS: Identification of specific subtype of SAPHO patient according to skin and nail manifestations as well as immunoglobulin E level may guide the selection of short-term systemic corticosteroids strategy, leading to remarkable long-term remission. Besides, the lesions on bone scintigraphy can hardly disappear in SAPHO patients, and instead, the quantitative analysis of bone scintigraphy and MRI performances may better reflect the change of disease condition and serve as indicator for treatment efficiency.


Assuntos
Síndrome de Hiperostose Adquirida/tratamento farmacológico , Glucocorticoides/administração & dosagem , Metilprednisolona/administração & dosagem , Prednisona/administração & dosagem , Administração Oral , Adulto , Biomarcadores/sangue , Osso e Ossos/diagnóstico por imagem , Osso e Ossos/patologia , Feminino , Humanos , Imunoglobulina E/sangue , Unhas/efeitos dos fármacos , Cintilografia , Indução de Remissão/métodos , Pele/efeitos dos fármacos
4.
J Photochem Photobiol B ; 197: 111515, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31255939

RESUMO

An extraordinary arrangement of research is as yet going on in the area of orthopedic implants advancement to determine different issues being looked by the engineering today. In spite of a few detriments of the orthopedic metallic inserts, they keep on being utilized, essentially as a result of their unrivaled mechanical properties. We investigated the conceivable utilization of silicon carbide (SiC) as a nano-ceramic covering material of titanium (Ti)-based all out femoral substitution implants. The thought is to keep wear garbage arrangement from the delicate titanium exterior. Silicon carbide is a hard and firmly holding bio-ceramic surface substance, and in light of these physico-chemical properties, it isn't actually degradable, just like the case with apatite (HA). To improve cytocompatibility and osseous-integration, we deposited anodized titanium nanotubes (TiO2) inserts, by electrochemical deposition method (EDM), with silicon carbide (SiC) with apatite (SiC@HA). The deposition was affirmed by SEM, while phase composition properties were assessed by XRD. Calcium affidavit, osteocalcin creation, and articulation of bone genes were essentially higher in rodent osteoblast cell culture on SiC@HA-covered anodized titanium nanotubes than in cells cultured on uncoated anodized titanium nanotubes. Implantation into rodent femurs likewise demonstrated that the SiC@HA-covered substance had unrivaled osseous-integration movement in correlation with that of customary inserts, as evaluated by in vivo tomography and histology. Therefore, anodized titanium nanotubes covered with SiC@HA holds guarantee as an orthopedic implant substance.


Assuntos
Regeneração Óssea , Compostos Inorgânicos de Carbono/química , Materiais Revestidos Biocompatíveis/química , Durapatita/química , Nanopartículas/química , Compostos de Silício/química , Titânio/química , Animais , Regeneração Óssea/efeitos dos fármacos , Osso e Ossos/metabolismo , Osso e Ossos/patologia , Adesão Celular/efeitos dos fármacos , Materiais Revestidos Biocompatíveis/farmacologia , Materiais Revestidos Biocompatíveis/uso terapêutico , Subunidade alfa 1 de Fator de Ligação ao Core/genética , Subunidade alfa 1 de Fator de Ligação ao Core/metabolismo , Fraturas do Fêmur/terapia , Regulação da Expressão Gênica/efeitos dos fármacos , Masculino , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Células-Tronco Mesenquimais/metabolismo , Osteoblastos/citologia , Osteoblastos/efeitos dos fármacos , Osteocalcina/metabolismo , Próteses e Implantes , Ratos
5.
J Biol Regul Homeost Agents ; 33(4): 1105-1111, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31332987

RESUMO

The adapter protein myeloid differentiation primary response gene 88 (MyD88) links the intracellular domains of interleukin receptors 1 and 18, and most Toll-like receptors (TLRs) to interleukin 1 receptor associated kinase (IRAK) signaling and subsequent NF-κB-mediated transcription. Previous work showed that mice with global deficiency of MyD88 (MyD88-/-) have osteopenic cancellous bone along with a reduction in osteoblastic but also osteoclastic surfaces. To further elucidate the role of MyD88 in bone, we utilized mice with osteoclast-restricted MyD88 expression in bone (MyD88OC). Bones of MyD88OC and wild type (wt) mice were examined by microCT analysis. Mechanical properties of bones were tested by three-point bending, and gene expression measured using quantitative real-time polymerase chain reaction. In MyD88OC mice, no osteopenic traits were observed, however, a drastic reduction in geometric parameters was detected. In trabecular bone a loss of connectivity density (-44%, p less than 0.0001) was measured and in cortical bone Imax (-31%, p less than 0.0001), Imin (-20%, p less than 0.001), J (-26%, p less than 0.0001) were reduced. Mechanical testing showed increased load to failure (77%, p less than 0.01) and decreased deflection at failure (-68%, p less than 0.01) of the femur. On the molecular level, relative gene expression analysis showed a (-29%, p less than 0.01) reduction in receptor activator of nuclear factor κ B ligand (RANKL) and no difference in osteoprotegerin (OPG) or RANK. Further, the bone resorption markers cathepsin K (CTSK) and tartrate-resistant acid phosphatase 5 (TRAP) were unchanged. In contrast, the bone formation markers collagen type 1 (COL1A1) and osteocalcin (OC) were decreased by -72% (p less than 0.0001) and -82% (p less than 0.0001), respectively. Together, our data suggests that the function of MyD88 in osteoclasts is sufficient to maintain bone mass, while it fails to preserve bone geometry, likely through dysfunctions in osteoblasts.


Assuntos
Reabsorção Óssea , Osso e Ossos/patologia , Fator 88 de Diferenciação Mieloide/metabolismo , Osteoclastos/citologia , Animais , Catepsina K/metabolismo , Diferenciação Celular , Colágeno Tipo I/metabolismo , Camundongos , Osteoblastos , Osteocalcina/metabolismo , Osteoclastos/metabolismo , Osteoprotegerina/metabolismo , Ligante RANK/metabolismo , Receptor Ativador de Fator Nuclear kappa-B/metabolismo , Fosfatase Ácida Resistente a Tartarato/metabolismo
6.
Forensic Sci Int ; 301: 118-128, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31153989

RESUMO

Forensic pathology and forensic anthropology are inherently related fields. The observations made during the autopsy and the conclusions from the anthropological analysis highlight the complementarities between the two approaches. In this review, gunshot wounds, sharp force injuries, chop wounds, blunt trauma, burned bodies, mechanical asphyxia and dismemberment will be analyzed from both perspectives. The result of this systematic review is that the conclusions stemming from the presence or absence of bone injuries must be very cautious because the observation of severe bone lesions does not always mean that this trauma was the cause of death; conversely, traumatic death, and even homicide, can be associated with a complete absence of bone lesions.


Assuntos
Osso e Ossos/lesões , Osso e Ossos/patologia , Antropologia Forense , Patologia Legal , Ferimentos e Lesões/patologia , Humanos
7.
Cancer Imaging ; 19(1): 42, 2019 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-31234926

RESUMO

BACKGROUND: To evaluate the value of CT-guided percutaneous needle biopsy of bone in the diagnosis of lymphomas based on PET/CT results. METHODS: A retrospective analysis of the records of all patients with percutaneous bone biopsies based on PET/CT results and a final diagnosis of lymphoma between January 2012 and August 2017 was performed. Thirty-one patients were included in this study. The success and complication rates were assessed. RESULTS: The mean age of the 31 patients was 46.6 ± 21.2 years, and there were 16 men and 15 women. A definite diagnosis and accurate histological subtype were obtained in 26 patients, for a success rate of 84%. The most common subtype was diffuse large B cell lymphoma (n = 18). The remaining subtypes included three cases of marginal-zone lymphoma, two cases of follicular lymphoma, one case of Hodgkin's lymphoma, one case of peripheral T cell lymphoma, and one case of B cell lymphoblastic lymphoma. No serious complications occurred in any of the patients. CONCLUSIONS: CT-guided needle biopsy based on PET/CT results is a reliable means of diagnosing and classifying lymphomas.


Assuntos
Osso e Ossos/patologia , Linfoma Difuso de Grandes Células B/patologia , Tomografia Computadorizada com Tomografia por Emissão de Pósitrons/métodos , Adulto , Idoso , Osso e Ossos/diagnóstico por imagem , Feminino , Humanos , Biópsia Guiada por Imagem/métodos , Linfoma Difuso de Grandes Células B/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade
8.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 33(6): 762-767, 2019 Jun 15.
Artigo em Chinês | MEDLINE | ID: mdl-31198007

RESUMO

Objective: To summarize the diagnosis and measurement methods of bone defect in anterior shoulder instability (glenoid bone defect and Hill-Sachs lesion). Methods: The related literature on the diagnosis and measurement of the bone defect in anterior shoulder instability was reviewed and summarized. Results: The commonly used techniques for the diagnosis of anterior glenoid bone defect and Hill-Sachs lesion of humeral head include X-ray, CT, MRI, arthroscopy, arthrography. The methods for measuring the degree of anterior glenoid bone defect include Griffith method, glenoid index method, Pico method, and best-fit circle method. The indexes for measuring the Hill-Sachs lesion include the length, width, depth, and volume. X-ray is mainly used for primary screening. Best-fit circle method on three-dimensional (3D) CT reconstruction is commonly used to measure the glenoid bone defect currently. Glenoid track theory on 3D CT reconstruction is popular in recent years. Reliability of measuring the glenoid bone defect and Hill-Sachs lesion with MRI and arthroscopy is still debatable. Arthrography is more and more used in the diagnosis of shoulder joint instability of bone defect and concomitant soft tissue injury. Conclusion: How to improve the accuracy of evaluating glenoid bone defect and Hill-Sachs lesion before surgery still need further study.


Assuntos
Osso e Ossos , Instabilidade Articular , Luxação do Ombro , Articulação do Ombro , Osso e Ossos/patologia , Humanos , Reprodutibilidade dos Testes , Ombro
9.
J Forensic Leg Med ; 66: 107-112, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31254968

RESUMO

More than 200 men were killed at the Koricani Cliffs on Mount Vlasic in central Bosnia during the Bosnian war. The location of this mass grave remained unknown for a long time following the war, until 2008, when the Missing Persons Institute discovered a site containing the remains of approximately 60 individuals. Later, in September 2017, a new mass grave was identified at this location that had not been robbed and skeletal remains remained close to the location where the victims had died. This grave was also unique, by definition, as it was a primary inhumation site, but with a high degree of commingling and disarticulation, typical of secondary inhumation locations. The exhumation team found the first remains in this grave approximately one and a half meters beneath the rocks, while the extent of the commingling resulted in necessary modifications to standardized exhumation protocols. The search and recovery process primarily focused on skulls, groups of bones that remained in clothing, and any bones that were still connected to each other. In total, 86 skulls, 137 groups of bones that had at least 2 bones connected, and a couple of hundred small bones that could not be appointed to individuals, were retrieved. The material was taken to the Sejkovaca Identification Centre where the team took over 1,300 DNA samples for analysis and are now awaiting the results.


Assuntos
Osso e Ossos/patologia , Exumação , Guerra , Osso e Ossos/química , Bósnia e Herzegóvina , Sepultamento , DNA/isolamento & purificação , Antropologia Forense , Humanos , Masculino , Solo
10.
Ann R Coll Surg Engl ; 101(6): 405-410, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31155889

RESUMO

INTRODUCTION: The aims of this study were to report the presenting characteristics and identify how best to distinguish bone and soft-tissue infections that mimic sarcomas. MATERIALS AND METHODS: A total of 238 (211 osteomyelitis and 27 soft-tissue infections) patients referred to a tertiary sarcoma multidisciplinary team with suspected sarcoma who were ultimately diagnosed with a bone or soft tissue infection were included. Data from a prospectively collated database was analysed retrospectively. RESULTS: Of all possible bone and soft-tissue sarcoma referrals, a diagnosis of infection was made in 2.1% and 0.7%, respectively. Median age was 18 years in the osteomyelitis group and 46 years in the soft-tissue infection group. In the osteomyelitis group, the most common presenting features were pain (85.8%) and swelling (32.7%). In the soft-tissue infection group, the most common clinical features were swelling (96.3%) and pain (70.4%). Those in the soft-tissue group were more likely to have raised inflammatory markers. Radiological investigations were unable to discern between tumour or infection in 59.7% of osteomyelitis and 81.5% of soft-tissue infection cases. No organism was identified in 64.9% of those who had a percutaneous biopsy culture. CONCLUSIONS: This study has highlighted that infection is frequently clinically indistinguishable from sarcoma and remains a principle non-neoplastic differential diagnosis. When patients are investigated for suspected sarcoma, infections can be missed due to falsely negative radiological investigations and percutaneous biopsy. As no single clinical, biochemical or radiological feature or investigation can be relied upon for diagnosis, clinicians should have a low threshold for tissue biopsy and discussion in a sarcoma multidisciplinary team meeting.


Assuntos
Neoplasias Ósseas/diagnóstico , Comunicação Interdisciplinar , Osteomielite/diagnóstico , Equipe de Assistência ao Paciente , Sarcoma/diagnóstico , Infecções dos Tecidos Moles/diagnóstico , Neoplasias de Tecidos Moles/diagnóstico , Biópsia , Neoplasias Ósseas/patologia , Osso e Ossos/patologia , Diagnóstico Diferencial , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Osteomielite/patologia , Estudos Retrospectivos , Sarcoma/patologia , Infecções dos Tecidos Moles/patologia , Neoplasias de Tecidos Moles/patologia
11.
Life Sci ; 229: 261-276, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31082400

RESUMO

AIM: Myokines are associated with regulation of bone and muscle mass. However, limited information is available regarding the impact of myokines on glucocorticoid (GC) mediated adverse effects on the musculoskeletal system. This study investigates the role of myokine fibroblast growth factor-2 (FGF-2) in regulating GC-induced deleterious effects on bone and skeletal muscle. METHODS: Primary osteoblast cells and C2C12 myoblast cell line were treated with FGF-2 and then exposed to dexamethasone (GC). FGF-2 mediated attenuation of the inhibitory effect of GC on osteoblast and myoblast differentiation and muscle atrophy was assessed through quantitative PCR and western blot analysis. Further, FGF-2 was administered subcutaneously to dexamethasone treated mice to collect bone and skeletal muscle tissue for in vivo analysis of bone microarchitecture, mechanical strength, histomorphometry and for histological alterations in treated tissue samples. KEY FINDINGS: FGF-2 abrogated the dexamethasone induced inhibitory effect on osteoblast differentiation by modulating BMP-2 pathway and inhibiting Wnt antagonist sclerostin. Further, dexamethasone induced atrophy in C2C12 cells was mitigated by FGF-2 as evident from down regulation of atrogenes expression. FGF-2 prevented GC-induced impairment of mineral density, biomechanical strength, trabecular bone volume, cortical thickness and bone formation rate in mice. Additionally, skeletal muscle tissue from GC treated mice displayed weak myostatin immunostaining and reduced expression of atrogenes following FGF-2 treatment. SIGNIFICANCE: FGF-2 mitigated GC induced effects through inhibition of sclerostin and myostatin expression in bone and muscle respectively. Taken together, this study exhibited the role of exogenous FGF-2 in sustaining osteoblastogenesis and inhibiting muscle atrophy in presence of glucocorticoid.


Assuntos
Osso e Ossos/metabolismo , Dexametasona/toxicidade , Fator 2 de Crescimento de Fibroblastos/farmacologia , Glicoproteínas/antagonistas & inibidores , Músculo Esquelético/metabolismo , Doenças Musculoesqueléticas/tratamento farmacológico , Miostatina/antagonistas & inibidores , Animais , Osso e Ossos/efeitos dos fármacos , Osso e Ossos/patologia , Diferenciação Celular , Células Cultivadas , Glucocorticoides/toxicidade , Camundongos , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/patologia , Doenças Musculoesqueléticas/induzido quimicamente , Doenças Musculoesqueléticas/metabolismo , Osteoblastos/citologia , Osteoblastos/efeitos dos fármacos , Osteoblastos/metabolismo , Osteogênese/efeitos dos fármacos
12.
Int J Mol Sci ; 20(9)2019 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-31075984

RESUMO

PURPOSE: The objective of this study was to assess the influence of a novel surface of dental implants (ContacTi®) on the osseointegration process in a minipig model. The surface was compared with other existing surfaces on the market (SLA® and SLActive®) by employing bone implant contact analysis (BIC) and implant stability. METHOD: Twelve minipigs were used with prior authorisation from an ethics committee. Three types of surfaces were tested: SLA® (sand-blasted acid-etched titanium), SLActive® (same but hydrophilic, performed under a nitrogen atmosphere), and ContacTi® (alumina particle bombardment of titanium, bioactivated when treated thermochemically) in 4.1 mm × 8 mm implants with internal connection and a polished neck. Twelve implants of each surface type (N = 36) were placed, sacrificing 1/3 of the animals at 2 weeks of placement, 1/3 at 4 weeks and the remaining 1/3 at 8 weeks. Numerical variables were compared with Analysis of Variance, and the correlation between ISQ and BIC was established with the Spearman's rank correlation coefficient. RESULTS: SLActive® and ContacTi® surfaces showed elevated osteoconductivity at 4 weeks, maintaining a similar evolution at 8 weeks (large amount of mature lamellar tissue with high maturity and bone quality). The SLA® surface showed slower maturation. The ISQ values in surgery were elevated (above 65), higher at necropsy and higher at 4 and 8 weeks in the SLA® group than in the other two (SLActive® and ContacTi®). No significant correlation was found between ISQ and BIC for each implant surface and necropsy time. CONCLUSION: The three surfaces analysed showed high RFA and BIC values, which were more favourable for the SLActive® and ContacTi® surfaces. No statistical correlation was found between the RFA and BIC values in any of the three surfaces analysed.


Assuntos
Materiais Biocompatíveis/farmacologia , Implantes Dentários , Osseointegração , Animais , Osso e Ossos/efeitos dos fármacos , Osso e Ossos/patologia , Feminino , Osseointegração/efeitos dos fármacos , Propriedades de Superfície , Suínos , Porco Miniatura
13.
BMC Infect Dis ; 19(1): 454, 2019 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-31117976

RESUMO

BACKGROUND: Mycolicibacterium phlei (M. phlei) is known to be a non-pathogenic nontuberculous mycobacterium (NTM) which rarely causes diseases in humans. A disseminated NTM infection is mostly caused by the Mycobacterium avium complex (MAC) and is known to develop in immunocompromised hosts, like those with acquired immune deficiency syndrome (AIDS). Here, we report a case of disseminated M. phlei infection in an immunocompetent host carrying anti-interferon gamma (IFN-γ) autoantibodies. CASE PRESENTATION: We detected M. phlei in multiple organs of an elderly woman with no significant medical history except positivity for anti-IFN-γ autoantibodies. She tested negative for human immunodeficiency virus (HIV)-1, 2/ Human T-cell leukemia virus type 1 (HTLV-1) antibody. High-resolution computed tomography (HRCT) of the chest demonstrated a nodule in the left S1 + 2 segment, interlobular septal thickening, multi lymphadenopathy, and osteolysis. A maximum intensity projection image following fluorodeoxyglucose-positron emission tomography (FDG-PET) revealed multifocal hypermetabolic lesions in the nodule and all the swollen lymph nodes seen in HRCT. FDG also accumulated in multiple bones. Advanced primary lung cancer was suspected, and biopsies of each lesion were performed. The pathology revealed caseating granuloma, positive for acid-fast bacteria, and DNA sequencing of the acid-fast bacteria confirmed the organism to be M. phlei. The patient also tested positive for anti-IFN-γ autoantibodies. Based on these findings, she was diagnosed with disseminated M. phlei infection, with anti-IFN-γ autoantibodies. CONCLUSIONS: Though known to be non-pathogenic, we show that M. phlei can be pathogenic like the MAC in immunocompetent individuals carrying anti-IFN-γ autoantibodies.


Assuntos
Interferon gama/imunologia , Infecções por Micobactéria não Tuberculosa/imunologia , Micobactérias não Tuberculosas/patogenicidade , Idoso , Antibacterianos/uso terapêutico , Autoanticorpos , Osso e Ossos/diagnóstico por imagem , Osso e Ossos/patologia , Feminino , Granuloma/diagnóstico por imagem , Granuloma/tratamento farmacológico , Granuloma/microbiologia , Humanos , Imunocompetência , Infecções por Micobactéria não Tuberculosa/diagnóstico por imagem , Infecções por Micobactéria não Tuberculosa/tratamento farmacológico , Infecções por Micobactéria não Tuberculosa/microbiologia , Micobactérias não Tuberculosas/imunologia , Tomografia por Emissão de Pósitrons/métodos , Tomografia Computadorizada por Raios X/métodos , Virulência
14.
Eur Radiol ; 29(7): 3424-3430, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31062134

RESUMO

OBJECTIVE: To evaluate contrast-enhanced T1-weighted magnetic resonance (MR) images in histologically proven fibrous dysplasia (FD) for the prevalence of "milk cloud appearance" and its association with ground-glass appearance (GGA) on radiography or computed tomography (CT). METHODS: For this retrospective cohort study, 37 patients with histologically proven FD imaged preoperatively with contrast-enhanced MR imaging and radiography or CT were identified at our institution. Three radiologists independently evaluated MR images for the presence of milk cloud appearance on T1-weighted contrast-enhanced images, sites of skeletal involvement, type of bone involved, uni- vs. multifocality, mono- vs. polyostotic disease, maximum diameter, proportion of bone involved, expansile remodeling, and T2 homogeneity. The presence or absence of GGA on radiography or CT was determined in consensus. Inter-reader agreement was evaluated for milk cloud appearance using Cohen's kappa, and associations between milk cloud appearance and other imaging parameters were tested using Spearman's rho. RESULTS: Among the 37 histologically proven FD lesions, GGA was identified in 70% of the lesions, while milk cloud appearance was found in 82% of the lesions. Inter-reader agreement for milk cloud appearance on MR imaging was good to excellent (κ 0.65, 0.82, and 0.8). A significant correlation was found between milk cloud appearance and GGA (ρ = 0.31, p < 0.001). CONCLUSION: Milk cloud appearance is a characteristic sign of FD on contrast-enhanced T1-weighted MR images. Recognition of this feature may be helpful when radiographs are equivocal or unremarkable or when MR imaging is performed as the primary imaging modality in cases of FD. KEY POINTS: • Fibrous dysplasia displays a characteristic feature on contrast-enhanced T1-weighted MR imaging: milk cloud appearance. • Milk cloud appearance correlates well with the radiographic or CT finding of ground-glass appearance. • Recognition of milk cloud appearance on contrast-enhanced MR imaging may be helpful when radiographs are equivocal or unremarkable or when MR imaging is performed as the primary imaging modality in cases of fibrous dysplasia.


Assuntos
Osso e Ossos/patologia , Meios de Contraste/farmacologia , Displasia Fibrosa Óssea/diagnóstico , Imagem por Ressonância Magnética/métodos , Adolescente , Adulto , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Adulto Jovem
15.
Cancer Biother Radiopharm ; 34(4): 258-263, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31070482

RESUMO

Background: Downregulation of LncRNA LINC-PINT has been observed in different types of cancer cells, indicating its role as a tumor suppressor. Materials and Methods: Expression of LINC-PINT and miRNA-21 in tumor tissues and adjacent healthy tissues of 56 patients with osteosarcoma was detected by real-time quantitative PCR. Correlations between expression levels of LncRNA LINC-PINT and miRNA-21 were analyzed by Pearson's correlation coefficient. Results: In this study, we found that LncRNA LINC-PINT was inhibited, whereas miRNA-21 was promoted in tumor tissues than in adjacent healthy tissues of patients with osteosarcoma. Expression levels of LncRNA LINC-PINT were affected by both tumor size and tumor metastasis. LncRNA LINC-PINT and miRNA-21 were significantly and reversely correlated in both tumor cells and adjacent healthy tissues. LncRNA LINC-PINT overexpression led to downregulated miRNA-21 expression in cancer cells, whereas miRNA-21 overexpression did not significantly affect LINC-PINT expression. Overexpression of LncRNA LINC-PINT inhibited whereas miRNA-21 overexpression promoted cancer cell proliferation, migration, and invasion. In addition, miRNA-21 overexpression partially rescued the inhibited cell proliferation, migration, and invasion mediated by LncRNA LINC-PINT overexpression. Conclusion: Therefore, LncRNA LINC-PINT may inhibit cancer cell proliferation, invasion, and migration in osteosarcoma by downregulating miRNA-21.


Assuntos
Regulação Neoplásica da Expressão Gênica , MicroRNAs/genética , Osteossarcoma/genética , RNA Longo não Codificante/metabolismo , Adolescente , Adulto , Osso e Ossos/patologia , Osso e Ossos/cirurgia , Moléculas de Adesão Celular/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Regulação para Baixo , Feminino , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/genética , Masculino , Invasividade Neoplásica/genética , Invasividade Neoplásica/patologia , Osteossarcoma/patologia , Osteossarcoma/cirurgia , Carga Tumoral/genética , Regulação para Cima , Adulto Jovem
16.
Cancer Biother Radiopharm ; 34(4): 264-270, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31070483

RESUMO

Purpose: Many studies have revealed that microRNAs (miRNAs) play crucial roles in cancer development and progression. miRNA-217 (miR-217) has been implicated in various cancers. However, the role of miR-217 in osteosarcoma (OS) remains unclear. In this study, the authors examined the role of miR-217 in development of OS. Materials and Methods: Using quantitative real-time PCR, they assessed expression levels of miR-217 in cultured cells and patient samples and examined the proliferation, migration, and invasion of cultured cells by MTT cell proliferation assays, cell scratch test, and cell transwell test. The proliferation, migration, and invasion were examined for MG63 and U2OS transfected by miR-217. Silent information regulator 2 homolog 1 (SIRT1) overexpression plasmid was designed. Results: Expression of miR-217 was downregulated in samples of OS tissue and cultured cells. Proliferation, migration, and invasion were inhibited by ectopic overexpression of miR-217. SIRT1 was identified as targets of miR-217. Expression levels of SIRT1 were inhibited by miR-217 expression in cultured cells, and the expression levels of miR-217 and SIRT1 were inversely correlated in patients with OS. Conclusion: MiR-217 acts as a tumor suppressor in the development of OS. The tumor-suppressive function of miR-217 in OS suggests inhibition of SIRT1.


Assuntos
Regulação Neoplásica da Expressão Gênica , MicroRNAs/metabolismo , Osteossarcoma/genética , Sirtuína 1/genética , Osso e Ossos/patologia , Osso e Ossos/cirurgia , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Regulação para Baixo , Humanos , Invasividade Neoplásica/genética , Invasividade Neoplásica/patologia , Osteossarcoma/patologia , Osteossarcoma/cirurgia , Regulação para Cima
17.
Anticancer Res ; 39(5): 2553-2559, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31092452

RESUMO

BACKGROUND/AIM: This study aimed to evaluate the therapeutic effect of enzalutamide (ENZ) or abiraterone acetate (ABI) on bone metastasis in castration-resistant prostate cancer (CRPC), using bone scan index (BSI). MATERIALS AND METHODS: Treatment outcomes for 31 patients who had undergone ENZ or ABI treatment were examined for CRPC with bone metastases. Cox proportional-hazards regression models were used to investigate the association between overall survival (OS) and clinical characteristics. RESULTS: Median OS after ENZ or ABI treatment was 29 months. Considering the flare phenomenon, BSI in 17 (55%) patients decreased following treatment. In multivariate analysis, low baseline BSI value and a decrease in BSI following treatment were associated with longer OS (hazard ratio [HR]=8.009; p=0.35 and HR=7.025; p=0.045*, respectively). CONCLUSION: Low BSI value before ENZ/ABI treatment and a decrease in BSI following ENZ or ABI treatment are independent predictors of longer OS. BSI could be useful for risk assessment of CRPC patients with bone metastases.


Assuntos
Neoplasias Ósseas/diagnóstico , Metástase Neoplásica/diagnóstico , Neoplasias de Próstata Resistentes à Castração/diagnóstico , Cintilografia/métodos , Acetato de Abiraterona/administração & dosagem , Idoso , Neoplasias Ósseas/diagnóstico por imagem , Neoplasias Ósseas/patologia , Neoplasias Ósseas/secundário , Osso e Ossos/diagnóstico por imagem , Osso e Ossos/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Metástase Neoplásica/diagnóstico por imagem , Metástase Neoplásica/patologia , Feniltioidantoína/administração & dosagem , Feniltioidantoína/análogos & derivados , Neoplasias de Próstata Resistentes à Castração/diagnóstico por imagem , Neoplasias de Próstata Resistentes à Castração/patologia
18.
Medicine (Baltimore) ; 98(19): e15546, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31083212

RESUMO

INTRODUCTION: Epithelioid hemangioendothelioma (EHE) of the bone is a very rare malignant vascular neoplasm, with biologic behavior between that of locally aggressive epithelioid hemangioma and malignant epithelioid angiosarcoma. We report a case of a patient with EHE who underwent bone scintigraphy, try to identify the characteristics of it, to highlight the clinical importance of whole-body bone scintigraphy and single-photon emission computed tomography/computed tomography (SPECT/ CT) in the diagnosis and treatment of EHE. PATIENT CONCERNS: A 67-year-old man with no history of trauma who reported pain in both lower limbs for 6 months, which had been worsening over the last 20 days. Anteroposterior and lateral radiographs of both lower limbs revealed numerous osteolytic lesions in the tibia and fibula bilaterally. Tc-methylene diphosphonate (MDP) bone scintigraphy demonstrated increased tracer uptake in the pelvic and bilateral lower limb bones. SPECT/CT bone imaging showed numerous osteolytic lesions cluster in the same anatomic region, with high tracer uptake in lesion margins. DIAGNOSIS: EHE of the bone. INTERVENTIONS: Two months after the diagnosis was confirmed, the patient was rehospitalized. Nonopioid analgesic use had not provided pain relief. Magnetic resonance imaging (MRI) of both thighs showed the bone cortex was destroyed with numerous irregular lesions, and soft-tissue was involved. A second bone scintigraphy did not show any new lesions. He was administrated with recombination human endostatin injection 15 mg ivgtt qd for 14 days, combined with apatinib mesylate tablets 500 mg po qd for 18 days. OUTCOMES: He was discharged voluntarily and died 2 months later. CONCLUSION: EHE of the bone is a very rare malignant vascular neoplasm with no specific radiographic imaging features. Whole-body bone scintigraphy, especially SPECT/CT bone imaging, significantly reduces ambiguous diagnoses and is recommended before treatment.


Assuntos
Neoplasias Ósseas/diagnóstico por imagem , Hemangioendotelioma Epitelioide/diagnóstico por imagem , Idoso , Neoplasias Ósseas/tratamento farmacológico , Neoplasias Ósseas/patologia , Osso e Ossos/diagnóstico por imagem , Osso e Ossos/patologia , Diagnóstico Diferencial , Evolução Fatal , Hemangioendotelioma Epitelioide/tratamento farmacológico , Hemangioendotelioma Epitelioide/patologia , Humanos , Imagem por Ressonância Magnética , Masculino , Tomografia Computadorizada com Tomografia Computadorizada de Emissão de Fóton Único , Imagem Corporal Total
19.
J Forensic Leg Med ; 65: 68-75, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31108434

RESUMO

The aim of this work is to discuss the frequency of traumatic bone injuries in a quite unique skeletal assemblage of enslaved people from Valle da Gafaria, Lagos, Portugal (15th-17th centuries). In all, 30 males, 58 females, and 15 individuals of unknown sex were included in the study. The skeletal remains were macroscopically observed for traumatic lesions. When present, the traumatic bone injuries were classified as having occurred ante or perimortem. The antemortem lesions were also studied through radiological analysis. Traumatic lesions were identified in 11 men (36.7%), 23 women (39.7%) and two individuals of unknown sex (13.3%). From these 36 individuals, 61.1% presented antemortem trauma, 25.0% perimortem trauma and 13.9% exhibited simultaneously ante and perimortem trauma. The mechanism of all traumatic injuries was blunt force trauma. From the 9965 analysed bones, 186 exhibited traumatic lesions (87 antemortem, 97 perimortem, and two with both ante and perimortem lesions). The bone more affected by antemortem trauma was the 5th right intermediate foot phalange (40.0%) and by perimortem trauma was the skull (11.4%), probably related to accidents and interpersonal violence, respectively. When analysed by sex, the only significant differences were found in the skull and the right 5th proximal foot phalanges, men (57.1%) presenting more lesions than women (15.4%). The obtained results are consistent with an arduous life, corroborating historical sources which document labour accidents, physical punishments and hard work in the populations of slaves.


Assuntos
Grupo com Ancestrais do Continente Africano/história , Osso e Ossos/lesões , Osso e Ossos/patologia , Escravização/história , Fraturas Ósseas/patologia , Adolescente , Adulto , Feminino , Antropologia Forense , História do Século XV , História do Século XVI , História do Século XVII , Humanos , Masculino , Pessoa de Meia-Idade , Portugal , Adulto Jovem
20.
Comput Methods Biomech Biomed Engin ; 22(7): 772-787, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30931618

RESUMO

Statistical shape models (SSM) of bony surfaces have been widely proposed in orthopedics, especially for anatomical bone modeling, joint kinematic analysis, staging of morphological abnormality, and pre- and intra-operative shape reconstruction. In the SSM computation, reference shape selection, shape registration and point correspondence computation are fundamental aspects determining the quality (generality, specificity and compactness) of the SSM. Such procedures can be made critical by the presence of large morphological dissimilarities within the surfaces, not only because of anthropometrical variability but also mainly due to pathological abnormalities. In this work, we proposed a SW pipeline for SSM construction based on pair-wise (PW) shape registration, which requires the a-priori selection of the reference shape, and on a custom iterative point correspondence algorithm. We addressed large morphological deformations in five different bony surface sets, namely proximal femur, distal femur, patella, proximal fibula and proximal tibia, extracted from a retrospective patient dataset. The technique was compared to a method from the literature, based on group-wise (GW) shape registration. As a main finding, the proposed technique provided generalization and specificity median errors, for all the five bony regions, lower than 2 mm. The comparative analysis provided basically similar results. Particularly, for the distal femur that was the shape affected by the largest pathological deformations, the differences in generalization, specificity and compactness were lower than 0.5 mm, 0.5 mm, and 1%, respectively. We can argue the proposed pipeline, along with the robust correspondence algorithm, is able to compute high-quality SSM of bony shapes, even affected by large morphological variability.


Assuntos
Osso e Ossos/patologia , Osso e Ossos/fisiopatologia , Processamento de Imagem Assistida por Computador , Modelos Anatômicos , Modelos Estatísticos , Idoso , Algoritmos , Humanos , Imagem Tridimensional/métodos , Estudos Retrospectivos
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