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1.
Life Sci ; 240: 117019, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31678554

RESUMO

AIMS: Long noncoding RNA melanotransferrin antisense RNA (MFI2-AS1) plays a vital role in the development of multiple diseases. This study aimed to investigate the effect of this lncRNA on osteoarthritis progression and explore the interaction among MFI2-AS1, microRNA (miR)-130a-3p and transcription factor 4 (TCF4). METHODS: Forty-six knee osteoarthritis tissues and 28 normal samples were collected. Human chondrocytes C28/I2 cells treated by lipopolysaccharide (LPS) were used as the model of osteoarthritis. The expression levels of MFI2-AS1, miR-130a-3p and TCF4 were detected by quantitative real-time polymerase chain reaction or western blot. LPS-induced chondrocytes injury was investigated by cell viability, apoptosis, inflammatory response and extracellular matrix degradation using MTT, flow cytometry, enzyme-linked immunosorbent assay and western blot. The target association between miR-130a-3p and MFI2-AS1 or TCF4 was confirmed by luciferase reporter assay and RNA immunoprecipitation. RESULTS: MFI2-AS1 expression was increased in osteoarthritis tissues and LPS-treated C28/I2 cells. Silence of MFI2-AS1 attenuated LPS-induced viability suppression, apoptosis production, inflammatory response and extracellular matrix degradation. MFI2-AS1 was validated as a decoy of miR-130a-3p and TCF4 was confirmed as a target of miR-130a-3p. miR-130a-3p overexpression inhibited LPS-induced cell injury in C28/I2 cells by decreasing TCF4 expression. Moreover, knockdown of MFI2-AS1 alleviated LPS-induced cell injury in C28/I2 cells by mediating miR-130a-3p and TCF4. CONCLUSION: Knockdown of MFI2-AS1 increased cell viability but suppressed apoptosis, inflammatory response and extracellular matrix degradation in LPS-treated chondrocytes by increasing miR-130a-3p and decreasing TCF4, indicating a novel target for the treatment of osteoarthritis.


Assuntos
Técnicas de Silenciamento de Genes , Lipopolissacarídeos , MicroRNAs/genética , Osteoartrite/genética , Osteoartrite/prevenção & controle , RNA Antissenso/genética , RNA Longo não Codificante/genética , Fator de Transcrição 4/genética , Animais , Linhagem Celular , Proliferação de Células , Sobrevivência Celular , Condrócitos , Humanos
2.
Gait Posture ; 74: 87-93, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31491565

RESUMO

BACKGROUND: Partial meniscectomy dramatically increases the risk for post-traumatic, tibiofemoral osteoarthritis after anterior cruciate ligament reconstruction (ACLR). Concomitant medial meniscus surgery influences walking biomechanics (e.g., medial tibiofemoral joint loading) early after ACLR; whether medial meniscus surgery continues to influence walking biomechanics two years after ACLR is unknown. RESEARCH QUESTION: Does medial meniscus treatment at the time of ACLR influence walking biomechanics two years after surgery? METHODS: This is a secondary analysis of prospectively collected data from a clinical trial (NCT01773317). Fifty-six athletes (age 24 ±â€¯8 years) with operative reports, two-year biomechanical analyses, and no second injury prior to two-year testing participated after primary ACLR. Participants were classified by concomitant medial meniscal status: no medial meniscus involvement (n = 36), partial medial meniscectomy (n = 9), and medial meniscus repair (n = 11). Participants underwent biomechanical analyses during over-ground walking including surface electromyography; a validated musculoskeletal model estimated medial compartment tibiofemoral contact forces. Gait variables were analyzed using 3 × 2 ANOVAs with group (medial meniscus treatment) and limb (involved versus uninvolved) comparisons. RESULTS: There was a main effect of group (p = .039) for peak knee flexion angle (PKFA). Participants after partial medial meniscectomy walked with clinically meaningfully smaller PKFAs in both the involved and uninvolved limbs compared to the no medial meniscus involvement group (group mean difference [95%CI]; involved: -4.9°[-8.7°, -1.0°], p = .015; uninvolved: -3.9°[-7.6°, -0.3°], p = .035) and medial meniscus repair group (involved: -5.2°[-9.9°, -0.6°], p = .029; uninvolved: -4.7°[-9.0°, -0.3°], p = .038). The partial medial meniscectomy group walked with higher involved versus uninvolved limb medial tibiofemoral contact forces (0.45 body weights, 95% CI: -0.01, 0.91 BW, p = 0.053) and truncated sagittal plane knee excursions, which were not present in the other two groups. SIGNIFICANCE: Aberrant gait biomechanics may concentrate high forces in the antero-medial tibiofemoral cartilage among patients two years after ACLR plus partial medial meniscectomy, perhaps explaining the higher osteoarthritis rates and offering an opportunity for targeted interventions. LEVEL OF EVIDENCE: Level III.


Assuntos
Lesões do Ligamento Cruzado Anterior , Reconstrução do Ligamento Cruzado Anterior , Traumatismos em Atletas/cirurgia , Meniscectomia/métodos , Meniscos Tibiais/cirurgia , Caminhada/fisiologia , Adolescente , Adulto , Lesões do Ligamento Cruzado Anterior/fisiopatologia , Lesões do Ligamento Cruzado Anterior/cirurgia , Atletas , Traumatismos em Atletas/fisiopatologia , Fenômenos Biomecânicos , Feminino , Marcha/fisiologia , Humanos , Masculino , Pessoa de Meia-Idade , Osteoartrite/prevenção & controle , Estudos Prospectivos , Adulto Jovem
3.
Acta Cir Bras ; 34(6): e201900604, 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31432995

RESUMO

PURPOSE: In view of the principal role of Toll-like receptor 4 (TLR4) in mediating sterile inflammatory response contributing to osteoarthritis (OA) pathogenesis, we used lipopolysaccharide (LPS), a known TLR4 activator, to clarify whether modulation of TLR4 contributed to the protective actions of intra-articular administration of curcumin in a classical rat OA model surgically induced by anterior cruciate ligament transection (ACLT). METHODS: The rats underwent ACLT and received 50µl of curcumin at the concentration of 1 mg mL-1 and 10 µg LPS by intra-articular injection once a week for 8 weeks. Morphological changes of the cartilage and synovial tissues were observed. Apoptotic chondrocytes were detected using TUNEL assay. The concentrations of IL-1ß and TNF-ɑ in synovial fluid were determined using ELISA kits. The mRNA and protein expression levels of TLR4 and NF-κB p65 were detected by real-time PCR and Western blotting, respectively. RESULTS: Intra-articular administration of curcumin significantly improved articular cartilage injury, suppressed synovial inflammation and down-regulated the overexpression of TLR4 and its downstream NF-κB caused by LPS-induced TLR4 activation in rat osteoarthritic knees. CONCLUSION: The data suggested that the inhibition of TLR4 signal might be an important mechanism underlying a protective effect of local curcumin administration on OA.


Assuntos
Ligamento Cruzado Anterior/cirurgia , Curcumina/farmacologia , Osteoartrite/prevenção & controle , Receptor 4 Toll-Like/metabolismo , Animais , Ligamento Cruzado Anterior/patologia , Western Blotting , Modelos Animais de Doenças , Ensaio de Imunoadsorção Enzimática , Injeções Intra-Articulares , Interleucina-1beta/metabolismo , Lipopolissacarídeos , Masculino , NF-kappa B/metabolismo , Osteoartrite/induzido quimicamente , Osteoartrite/metabolismo , Reação em Cadeia da Polimerase , Ratos , Receptor 4 Toll-Like/efeitos dos fármacos , Fator de Necrose Tumoral alfa/metabolismo
5.
Oxid Med Cell Longev ; 2019: 4695381, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31231454

RESUMO

Tricetin is a well-studied flavonoid with a wide range of pharmacological activities in cancer and inflammation. However, the ability of tricetin to ameliorate the inflammation that occurs in osteoarthritis (OA) has not been determined. This study explored the effects of tricetin on interleukin- (IL-) 1ß-induced rat chondrocytes. Chondrocytes harvested from rat cartilage were incubated in vitro with tricetin in the presence of IL-1ß. The expression of matrix metalloproteinase- (MMP-) 1, MMP-3, MMP-13, nitric oxide (NO), prostaglandin E2 (PGE2), Bax, and Bcl-2 was evaluated by real-time-PCR, ELISA, Griess reaction, and western blotting. Caspase-3 activity in chondrocytes was determined using a caspase-3 activity assay and MAPK pathway activity by western blotting. Tricetin decreased the expression of MMP-1, MMP-3, and MMP-13 at both the gene and protein level in IL-1ß-induced rat chondrocytes. It also inhibited IL-1ß-induced NO and PGE2 production, by modulating inducible NO synthase and cyclooxygenase 2 gene expression. An antiapoptotic role of tricetin involving the Bax/Bcl-2/caspase-3 pathway was also determined. The chondroprotective effect of tricetin was shown to be partly related to the suppression of the MAPK signaling pathway. The results of this study demonstrate the chondroprotective role of tricetin, based on its anticatabolic, anti-inflammatory, and antiapoptotic effects in chondrocytes. The therapeutic potential of tricetin in OA patients should be explored in future studies.


Assuntos
Anti-Inflamatórios/farmacologia , Apoptose/efeitos dos fármacos , Condrócitos/efeitos dos fármacos , Cromonas/farmacologia , Inflamação/prevenção & controle , Interleucina-1beta/toxicidade , Osteoartrite/prevenção & controle , Substâncias Protetoras/farmacologia , Animais , Condrócitos/metabolismo , Condrócitos/patologia , Feminino , Inflamação/induzido quimicamente , Inflamação/metabolismo , Inflamação/patologia , Masculino , Osteoartrite/induzido quimicamente , Osteoartrite/metabolismo , Osteoartrite/patologia , Ratos , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos
6.
Inflammation ; 42(5): 1754-1766, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31201586

RESUMO

Osteoarthritis (OA), which is characterized as a common degenerative joint disease, is presently the most prevalent chronic degenerative joint disease. Accumulating evidence has shown a biological function for Garcinol in a variety of diseases; however, whether it could be used to treat OA remains unclear. In this study, we explored the protective effects of garcinol on the progression of OA and explored the underlying mechanism. In vitro, garcinol reduced the expression of pro-inflammatory cytokines, such as IL-6 and tumor necrosis factor alpha (TNF-α). It also decreased the expression of inducible nitric oxide synthase (iNOS), as well as cyclooxygenase-2 (COX-2). Furthermore, garcinol inhibited the expression of thrombospondin motifs 5(ADAMTS5) and metalloproteinase (MMPs), both of which regulate extracellular matrix degradation. These changes could be attributed to garcinol-related suppression of the IL-1ß-induced NF-κB signaling pathway. Moreover, we investigated the protective effects of garcinol on the surgical destabilization of the medial meniscus (DMM) of the mouse, an in vivo model of OA. Taken together, our data suggest garcinol as a potential future agent for the treatment of OA.


Assuntos
Condrócitos/patologia , Inflamação/prevenção & controle , NF-kappa B/antagonistas & inibidores , Osteoartrite/prevenção & controle , Terpenos/farmacologia , Animais , Células Cultivadas , Ciclo-Oxigenase 2/metabolismo , Citocinas/metabolismo , Humanos , Inflamação/induzido quimicamente , Interleucina-1beta/farmacologia , Camundongos , Óxido Nítrico Sintase Tipo II/metabolismo , Osteoartrite/metabolismo , Transdução de Sinais/efeitos dos fármacos , Terpenos/uso terapêutico
7.
Wiad Lek ; 72(5 cz 2): 1064-1067, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31175745

RESUMO

OBJECTIVE: Introduction: Medico-social significance of osteoarthritis is due to a number of factors, one of which is associated with the need for long-term anti-inflammatory therapy, which is associated with undesirable side effects. The aim: Identify the features of the course of chronic gastritis in patients taking selective NSAIDs for osteoarthritis. PATIENTS AND METHODS: Materials and methods: Were examined 122 patients with osteoarthrosis, who had verified chronic gastritis in the anamnesis (50 males and 72 females), aged from 42 to 64. Control group included 40 patients with osteoarthrosis without gastroduodenal zone pathology in the anamnesis. For arthralgia relief, patients were prescribed to intake meloxicam (average dose - 12.5±1.39 mg/day) or nimesulide (average dose - 150±14.91 mg/day). RESULTS: Results: It was determined that prescription of selective NSAIDs (meloxicam and nimesulide) raised the risk of NSAIDs gastropathy/dyspepsia in 2.9 times (P<0.03) in patients with chronic gastritis in the anamnesis than in patients without associated gastroduodenal zone pathology. Atrophy of gastric mucous membrane was associated with higher risks (P>0.05) of erosive gastropathy. CONCLUSION: Conclusions: With the purpose of gastropathy prevention upon taking NSAIDs, patients with chronic gastritis in the anamnesis are recommended to undergo gastroprotective therapy.


Assuntos
Gastrite , Osteoartrite , Adulto , Anti-Inflamatórios não Esteroides , Comorbidade , Feminino , Gastrite/complicações , Humanos , Masculino , Pessoa de Meia-Idade , Osteoartrite/complicações , Osteoartrite/epidemiologia , Osteoartrite/prevenção & controle , Prevenção Secundária , Valores Sociais
10.
J Orthop Surg Res ; 14(1): 118, 2019 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-31053150

RESUMO

OBJECTIVE: Osteoarthritis (OA) is a prevalent degenerative disease caused by various factors. MicroRNAs are important regulators in OA. MiR-21-5p expression is decreased in OA cartilage, but the effects of modulating miR-21-5p on cartilage regeneration are unknown. Therefore, our aim was to investigate the effects of miR-21-5p on cartilage metabolism of OA chondrocytes. DESIGN: We used IL-1ß (10 ng/ml) to mimic OA chondrocytes. OA chondrocytes were transfected with miR-21-5p, the gene expression of COL2A1, MMP13, and ADAMTS5 was detected by qPCR. At the same time, COL2A1, MMP13, and ADAMTS5 were analyzed at the protein level by Western blot. CCK8 measured the cell's viability and SA-ß-gal detected the cell's senescence. RESULTS: Upregulation of miR-21-5p had increased COL2A1 expression and decreased MM P13 and ADAMTS5 expression, which were in accord with Western blot data. SA-ß-gal activity significantly increased, the viability was decreased in OA chondrocytes, and upregulation of miR-21-5p can decrease the SA-ß-gal activity and increase cell viability. CONCLUSION: MiR-21-5p might be a potential disease-modifying compound in OA, as it promotes hyaline cartilage production. These results provided that novel insights into the important function in OA pathological development.


Assuntos
Condrócitos/metabolismo , Interleucina-1beta/farmacologia , MicroRNAs/biossíntese , MicroRNAs/farmacologia , Osteoartrite/metabolismo , Adulto , Células Cultivadas , Condrócitos/efeitos dos fármacos , Condrócitos/patologia , Feminino , Humanos , Masculino , MicroRNAs/uso terapêutico , Pessoa de Meia-Idade , Osteoartrite/patologia , Osteoartrite/prevenção & controle
11.
Orthop Clin North Am ; 50(3): 401-414, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31084843

RESUMO

Poor clinical results are seen with syndesmotic injuries in the setting of ankle sprains and ankle fractures. The goal of syndesmosis repair is to restore the normal anatomic relationship of the distal tibiofibular joint and prevent ankle arthritis. Indications for surgical intervention for isolated syndesmotic injuries include frank syndesmosis diastasis, medial clear space widening on plain radiographs, significant radiographic syndesmosis diastasis during stress examination, or subtle syndesmotic diastasis detected by arthroscopic evaluation. Complications after syndesmosis repair include symptomatic hardware, malreduction, and arthritis. Anatomic reduction of the syndesmosis leads to better outcomes following surgery.


Assuntos
Fraturas do Tornozelo/cirurgia , Traumatismos do Tornozelo/cirurgia , Ligamentos Articulares/lesões , Ligamentos Articulares/cirurgia , Fraturas do Tornozelo/diagnóstico por imagem , Traumatismos do Tornozelo/diagnóstico por imagem , Artroscopia , Parafusos Ósseos , Contraindicações de Procedimentos , Fixação Interna de Fraturas/efeitos adversos , Humanos , Ligamentos Articulares/diagnóstico por imagem , Osteoartrite/prevenção & controle , Cuidados Pós-Operatórios , Complicações Pós-Operatórias/prevenção & controle , Radiografia
12.
Inflammation ; 42(4): 1426-1440, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30937838

RESUMO

In the present study, we demonstrated the anti-catabolic effects of formononetin, a phytoestrogen derived from herbal plants, against interleukin-1ß (IL-1ß)-induced severe catabolic effects in primary rat chondrocytes and articular cartilage. Formononetin did not affect the viability of primary rat chondrocytes in both short- (24 h) and long-term (21 days) treatment periods. Furthermore, formononetin effectively antagonized the IL-1ß-induced catabolic effects including the decrease in proteoglycan content, suppression of pericellular matrix formation, and loss of proteoglycan through the decreased expression of cartilage-degrading enzymes like matrix metalloproteinase (MMP)-13, MMP-1, and MMP-3 in primary rat chondrocytes. Moreover, catabolic oxidative stress mediators like nitric oxide, inducible nitric oxide synthase, cyclooxygenase-2, and prostaglandin E2 were significantly downregulated by formononetin in primary rat chondrocytes treated with IL-1ß. Sequentially, the upregulation of pro-inflammatory cytokines (like IL-1α, IL-1ß, IL-6, and tumor necrosis factor α), chemokines (like fractalkine, monocyte chemoattractant protein-1, and macrophage inflammatory protein-3α), and vascular endothelial growth factor were significantly downregulated by formononetin in primary rat chondrocytes treated with IL-1ß. These data suggest that formononetin may suppress IL-1ß-induced severe catabolic effects and osteoarthritic condition. Furthermore, formononetin may be a promising candidate for the treatment and prevention of osteoarthritis.


Assuntos
Condrócitos/patologia , Antagonismo de Drogas , Inflamação/tratamento farmacológico , Interleucina-1beta/farmacologia , Isoflavonas/farmacologia , Metabolismo/efeitos dos fármacos , Animais , Cartilagem Articular/efeitos dos fármacos , Células Cultivadas , Interleucina-1beta/antagonistas & inibidores , Isoflavonas/antagonistas & inibidores , Osteoartrite/prevenção & controle , Fitoestrógenos/farmacologia , Ratos
13.
PLoS One ; 14(4): e0215664, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31002692

RESUMO

Senna species and anthraquinone derivatives generated by these organisms, rhein and aloe-emodin, exert anti-inflammatory effects. These species present a similar morphology but produce different ingredients when they are used as medicinal products. In this study, a DNA barcoding- (Bar-) high-resolution melting (HRM) technique was developed using internal transcribed sequence 2 (ITS2) to differentiate between Senna alata and Senna tora as a result of significant differences in their melting profiles. We used this approach for confirmation of S. alata and S. tora raw materials, and we examined the chondroprotective properties of the ethanolic extracts of S. alata and S. tora using a porcine model of cartilage degradation induced by a combination of interleukin-17A (IL-17A) and IL-1ß. We found that both Senna ethanolic extracts, at a concentration of 25 µg/mL, effectively prevented cartilage degradation. Rhein and aloe-emodin were present in the extract of S. alata but not in that of S. tora. We observed a reduction in the release of sulfated glycosaminoglycans (S-GAGs) and hyaluronic acid (HA) into media in both treatments of Senna extracts, which indicated proteoglycan preservation in explant tissues. These results suggest that neither rhein nor aloe-emodin are the main factors responsible for cartilage-protecting properties. Taken together, results show that both S. alata and S. tora are promising for further development as anti-osteoarthritic agents and that Bar-HRM using ITS2 could be applied for species confirmation with Senna products.


Assuntos
Cartilagem/efeitos dos fármacos , Osteoartrite/prevenção & controle , Extrato de Senna/farmacologia , Senna (Planta)/química , Animais , Sequência de Bases , Cartilagem/metabolismo , Cartilagem/patologia , Colágeno Tipo II/metabolismo , Código de Barras de DNA Taxonômico/métodos , DNA Espaçador Ribossômico/genética , Modelos Animais de Doenças , Etanol/química , Osteoartrite/metabolismo , Fitoterapia/métodos , Substâncias Protetoras/farmacologia , Proteoglicanas/metabolismo , Extrato de Senna/química , Senna (Planta)/classificação , Senna (Planta)/genética , Homologia de Sequência do Ácido Nucleico , Especificidade da Espécie , Suínos
14.
Infect Immun ; 87(6)2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-30936160

RESUMO

Osteoarticular brucellosis is the most common complication in Brucella-infected humans regardless of age, sex, or immune status. The mechanism of bone destruction caused by Brucella species remained partially unknown due to the lack of a suitable animal model. Here, to study this complication, we explored the suitability of the use of the NOD-scid IL2rγnull mouse to study osteoarticular brucellosis and examined the potential use of this strain to evaluate the safety of live attenuated vaccine candidates. Mice were inoculated intraperitoneally with a single dose of 1 × 104, 1 × 105, or 1 × 106 CFU of B. abortus S19 or the vaccine candidate B. abortus S19ΔvjbR and monitored for the development of side effects, including osteoarticular disease, for 13 weeks. Decreased body temperature, weight loss, splenomegaly, and deformation of the tails were observed in mice inoculated with B. abortus S19 but not in those inoculated with S19ΔvjbR Histologically, all S19-inoculated mice had a severe dose-dependent inflammatory response in multiple organs. The inflammatory response at the tail was characterized by the recruitment of large numbers of neutrophils, macrophages, and osteoclasts with marked bone destruction. These lesions histologically resembled what is typically observed in Brucella-infected patients. In contrast, mice inoculated with B. abortus S19ΔvjbR did not show significant bone changes. Immunofluorescence, in situ hybridization, and confocal imaging demonstrated the presence of Brucella at the sites of inflammation, both intra- and extracellularly, and large numbers of bacteria were observed within mature osteoclasts. These results demonstrate the potential use of the NOD-scid IL2rγnull mouse model to evaluate vaccine safety and further study osteoarticular brucellosis.


Assuntos
Vacina contra Brucelose/administração & dosagem , Brucella abortus/imunologia , Brucelose/prevenção & controle , Osteoartrite/prevenção & controle , Animais , Vacina contra Brucelose/genética , Vacina contra Brucelose/imunologia , Brucella abortus/genética , Brucelose/imunologia , Brucelose/microbiologia , Brucelose/patologia , Modelos Animais de Doenças , Feminino , Humanos , Macrófagos/imunologia , Camundongos , Camundongos Endogâmicos NOD , Camundongos SCID , Osteoartrite/imunologia , Osteoartrite/microbiologia , Osteoartrite/patologia , Vacinas Atenuadas/administração & dosagem , Vacinas Atenuadas/genética , Vacinas Atenuadas/imunologia
15.
Biochem Pharmacol ; 165: 24-32, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-30825432

RESUMO

Osteoarthritis (OA) is a chronic joint disease characterized by cartilage degradation, osteophyte formation, subchondral bone sclerosis, and synovitis. Systemic factors such as obesity and the components of the metabolic syndrome seem to contribute to its progression. Breakdown of cartilage ensues from an altered balance between mechanical overload and its absorption by this tissue. There is in this context a status of persistent local inflammation by means of the chronic activation of innate immunity. A broad variety of danger-associated molecular patterns inside OA joint are able to activate pattern recognition receptors, mainly TLR (toll-like receptor) 2 and 4, which are overexpressed in the OA cartilage. Chronic activation of innate immune responses in chondrocytes results in a robust production of pro-inflammatory cytokines and chemokines, as well as of tissue-destructive enzymes, downstream of NF-κB and MAPK (mitogen activated protein kinase) dependent pathways. Besides, the toxic effects of an excess of glucose and/or fatty acids, which share the same pro-inflammatory intracellular signalling pathways, may add fuel to the fire. Not only high concentrations of glucose can render cells prone to inflammation, but also AGEs (advanced glycation end products) are integrated into the TLR signalling network through their own innate immune receptors. Considering these mechanisms, we argue for the control of both primary inflammation and proteolytic catabolism as a preventive strategy in OA, instead of focusing treatment on the enhancement of anabolic responses. Even though this approach would not return to normal already degraded cartilage, it nonetheless might avoid damage extension to the surrounding tissue.


Assuntos
Imunidade Inata , Inflamação/complicações , Osteoartrite/prevenção & controle , Animais , Condrócitos/imunologia , Progressão da Doença , Produtos Finais de Glicação Avançada/fisiologia , Humanos , Proteína 3 que Contém Domínio de Pirina da Família NLR/fisiologia , Osteoartrite/etiologia , Receptores Toll-Like/fisiologia
16.
J Athl Train ; 54(3): 270-275, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30829538

RESUMO

CONTEXT: Individuals with an anterior cruciate ligament reconstruction (ACLR) are at an increased risk of developing posttraumatic osteoarthritis. How osteoarthritis risk factors, such as increased body mass index (BMI), may influence early changes in joint tissue metabolism is unknown. OBJECTIVE: To determine the association between BMI and type 2 cartilage turnover in individuals with an ACLR. DESIGN: Cross-sectional study. SETTING: Research laboratory. PATIENTS OR OTHER PARTICIPANTS: Forty-five individuals (31 women, 14 men) with unilateral ACLR at least 6 months earlier who were cleared for unrestricted physical activity. MAIN OUTCOME MEASURE(S): Body mass index (kg/m2) and type 2 collagen turnover were the primary outcomes. Body mass index was calculated from objectively measured height and mass. Serum was obtained to measure type 2 collagen turnover, quantified as the ratio of degradation (collagen type 2 cleavage product [C2C]) to synthesis (collagen type 2 C-propeptide [CP2]; C2C : CP2). Covariate measures were physical activity level before ACLR (Tegner score) and current level of disability (International Knee Documentation Committee Index score). Associations of primary outcomes were analyzed for the group as a whole and then separately for males and females. RESULTS: Overall, greater BMI was associated with greater C2C : CP2 (r = 0.32, P = .030). After controlling for covariates (Tegner and International Knee Documentation Committee Index scores), we identified a similar association between BMI and C2C : CP2 (partial r = 0.42, P = .009). Among women, greater BMI was associated with greater C2C : CP2 before (r = 0.47, P = .008) and after (partial r = 0.50, P = .008) controlling for covariates. No such association occurred in men. CONCLUSIONS: Greater BMI may influence greater type 2 collagen turnover in those with ACLR. Individuals, especially women, who maintain or reduce BMI may be less likely to demonstrate greater type 2 collagen turnover ratios after ACLR.


Assuntos
Lesões do Ligamento Cruzado Anterior , Reconstrução do Ligamento Cruzado Anterior/reabilitação , Colágeno Tipo II , Articulação do Joelho/metabolismo , Osteoartrite , Adulto , Lesões do Ligamento Cruzado Anterior/complicações , Lesões do Ligamento Cruzado Anterior/diagnóstico , Lesões do Ligamento Cruzado Anterior/metabolismo , Lesões do Ligamento Cruzado Anterior/cirurgia , Reconstrução do Ligamento Cruzado Anterior/métodos , Índice de Massa Corporal , Colágeno Tipo II/sangue , Colágeno Tipo II/metabolismo , Estudos Transversais , Exercício/fisiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Osteoartrite/diagnóstico , Osteoartrite/etiologia , Osteoartrite/prevenção & controle , Prognóstico
17.
Ultrasound Med Biol ; 45(4): 944-953, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30732913

RESUMO

The aim of this study was to assess the effect of low-intensity pulsed ultrasound (LIPUS) application on rat temporomandibular joints (TMJs) with early-stage of osteoarthritis-like conditions induced by mechanical overloading. Fifteen-week-old male Wistar rats were divided into two experimental groups and a control group (n = 10 each). Both TMJs of all rats in one experimental group were subjected to mechanical overloading for 5 d, and those in the other experimental group were exposed to LIPUS for 20 min/d after overloading. Condyles were assessed using micro-computed tomography, histology and histomorphometry. LIPUS treatment attenuated cartilage degeneration, decreased the number of osteoclastic cells and restored the expression of aggrecan after an initial decrease induced by mechanical overloading. These results indicate that LIPUS may have a protective effect on the early progression of TMJ osteoarthritis.


Assuntos
Cartilagem Articular/fisiopatologia , Côndilo Mandibular/fisiopatologia , Osteoartrite/prevenção & controle , Estresse Mecânico , Terapia por Ultrassom/métodos , Animais , Modelos Animais de Doenças , Masculino , Ratos , Ratos Wistar , Ondas Ultrassônicas
18.
Maturitas ; 122: 35-43, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30797528

RESUMO

Osteoarthritis is the most prevalent chronic inflammatory joint disease affecting mobility in humans, as well as in companion and captive animals. Understanding the effect of dietary phytochemical intake from foods on osteoarthritis and its long-term outcomes may inform public health strategies for osteoarthritis prevention and management, reducing healthcare costs globally. The aim of this systematic review was to examine the effects of dietary phytochemical intake from foods on osteoarthritis in adult populations. A literature search was performed using Scopus, Web of Science, MEDLINE, PubMed and the Cochrane Library for human studies to identify randomised controlled trials (RCTs) and observational studies focused on osteoarthritis up to May 2018. From 5879 articles, five RCTs and four cross-sectional studies were identified. Dietary carotenoids were examined in the observational studies, while dietary intakes of polyphenols from foods were assessed in the RCTs. Dietary polyphenol intake from foods (e.g., freeze-dried strawberries and tart cherry juice) may slow the progression of osteoarthritis via decreased inflammation and reduced cartilage degradation. However, there were relatively few studies and a lack of uniformity in the biomarkers used and the measurements of pain, quality of life and physical activity relating to osteoarthritis. The heterogeneity among the studies suggests that there is insufficient evidence related to phytochemical intake from foods. High-quality epidemiological studies and controlled trials are therefore required. Nevertheless, exploring dietary phytochemical intake from foods may complement current dietary strategies for the management of osteoarthritis and help in the formulation of more economical and manageable strategies for osteoarthritis.


Assuntos
Osteoartrite/dietoterapia , Osteoartrite/prevenção & controle , Compostos Fitoquímicos/administração & dosagem , Animais , Humanos
20.
Nutrients ; 11(2)2019 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-30678273

RESUMO

Older adults are recommended vitamin D to prevent fractures. Though this population is also at risk of osteoarthritis (OA), the effect of vitamin D on OA is unclear and may differ by disease state. The relationship between vitamin D and OA during OA initiation and progression were considered in this narrative review of in vivo and in vitro studies. Regarding OA initiation in humans, the small number of published observational studies suggest a lack of association between induction of OA and vitamin D status. Most randomized controlled trials were performed in White OA patients with relatively high vitamin D status (>50 nmol/L). These studies found no benefit of vitamin D supplementation on OA progression. However, subset analyses and one randomized controlled pilot trial indicated that vitamin D supplementation may alleviate joint pain in OA patients with low vitamin D status (<50 nmol/L). As the etiology of OA is recently being more fully uncovered, better animal and cell models are needed. According to currently available clinical results, evidence is lacking to set a vitamin D level to prevent OA, and increasing vitamin D status above 50 nmol/L does not seem to benefit OA patients.


Assuntos
Osteoartrite/prevenção & controle , Vitamina D/administração & dosagem , Vitamina D/farmacologia , Envelhecimento , Humanos , Osteoartrite/tratamento farmacológico
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