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1.
Oncology ; 98(2): 81-90, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31509843

RESUMO

OBJECTIVES: The histologic response to chemotherapy is an important prognostic factor in osteosarcoma. Thus, we attempted to develop an effective neoadjuvant regimen to achieve an improvement in histologic response. METHODS: Twenty-nine patients with a high-grade osteosarcoma received 2 courses of neoadjuvant chemotherapy non-randomly with either the MAP regimen (methotrexate 12 g/m2, cisplatin 120 mg/m2, and doxorubicin 75 mg/m2) or MAPI regimen (MAP plus ifosfamide 9 g/m2). We applied interval compression to MAPI by shortening the preoperative period to be aligned with that of MAP. Adjuvant chemotherapy was tailored according to the necrosis rate of resected tumor specimens. Necrosis rate, toxicity, and survival outcome were compared retrospectively between the 2 groups. RESULTS: The median interval between the beginning of neoadjuvant chemotherapy and surgery was 97.0 days in the MAPI group (17 patients) and 90.5 days in the MAP group (12 patients; p = 0.19). The good histologic response (>90% of necrosis) was observed in 71% of MAPI and in 42% of MAP (p = 0.12). Major toxicities of grade 3 or worse were not different between the 2 groups. The probability of 5-year progression-free survival and overall survival of the MAPI group were 74 and 83%, and those in the MAP group were 50 and 75%, showing no difference. CONCLUSIONS: Interval-compressed MAPI therapy given in a similar duration of the preoperative phase to that of conventional MAP therapy showed a marginal trend toward a better histologic response without a significant increase in major toxicities. Regarding the proportion of good histologic response, 71% is one of the highest values ever reported in the literature. The results warrant further testing in a prospective way in a larger cohort.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Neoplasias Ósseas/diagnóstico , Neoplasias Ósseas/tratamento farmacológico , Osteossarcoma/diagnóstico , Osteossarcoma/tratamento farmacológico , Cuidados Pré-Operatórios , Adolescente , Adulto , Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos , Medula Óssea/patologia , Neoplasias Ósseas/mortalidade , Neoplasias Ósseas/cirurgia , Criança , Esquema de Medicação , Feminino , Humanos , Masculino , Estadiamento de Neoplasias , Osteossarcoma/mortalidade , Osteossarcoma/cirurgia , Cooperação do Paciente , Prognóstico , Modelos de Riscos Proporcionais , Estudos Retrospectivos , Resultado do Tratamento , Adulto Jovem
2.
BMC Cancer ; 19(1): 993, 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31646993

RESUMO

BACKGROUND: Limited evidence is available regarding the dissemination of tumor tissues due to compression during massage therapy, a routine procedure in patients with various symptoms in Asian countries. CASE PRESENTATION: A 12-year-old male presented at a massage clinic with pain and swelling of his left knee, which worsened the same night. Consistent with conventional osteosarcoma, radiography revealed cortical bone destruction, osteoblastic changes, and periosteal reactions. Magnetic resonance imaging revealed a tumor in the distal femur, an extraskeletal mass, and an infiltrative lesion in the intramuscular and neurovascular areas surrounding the distal femur; this was considered as hemorrhage and dissemination of the tumor tissue. 18Fluorine-labelled fluorodeoxyglucose-positron emission tomography and computed tomography revealed multiple metastases in the spine, liver, and lung. Consistent with osteosarcoma, histopathological examination revealed tumor cell proliferation with extensive pleomorphism and mitoses. Despite undergoing chemotherapy, radiation therapy, and hip disarticulation, the patient died due to multiple metastases 13 months after the initial diagnosis. CONCLUSIONS: The present case suggests association of massage therapy with the local dissemination of tumor tissues, although influence of massage therapy on metastatic lesions remains unclear. Massage therapists should be aware of the possibility for dissemination of hidden malignancies due to the procedure.


Assuntos
Neoplasias Ósseas/patologia , Massagem/efeitos adversos , Osteossarcoma/secundário , Neoplasias Ósseas/diagnóstico , Neoplasias Ósseas/terapia , Criança , Evolução Fatal , Humanos , Masculino , Osteossarcoma/diagnóstico , Osteossarcoma/terapia
3.
Artif Cells Nanomed Biotechnol ; 47(1): 1859-1866, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31397185

RESUMO

Background: The purpose of our research was to explore potential value of lncRNA-BC050642 in osteosarcoma origination and prognosis. Methods: In this study, the tissue specimens were collected from 97 osteosarcoma patients and 97 age-matched healthy controls. Besides, human osteosarcoma cell lines U2OS and normal osteoblastic cell line hFOB1.19 were selected for experiments in vitro. lncRNA-BC050642 levels were measured through quantitative real-time polymerase chain reaction (qRT-PCR). Relative expression of the gene c-myc was determined via ELISA analysis. x2 test was implemented to appraise possible relationship between BC050642 level and clinicopathological features. Cell proliferation assay, plate colony formation assay, and cell apoptosis assay were adopted to analyze the influence of BC050642 on tumor development. Besides, prognostic value of BC050642 was estimated using Kaplan-Meier and cox regression analysis. Results: BC050642 levels showed distinctive increases in osteosarcoma tissues and cell lines compared with controls. And c-myc expression was down-regulated in osteosarcoma. There was a negative correlation between the expressions of BC050642 and c-myc. BC050642 expression was proved to be significantly correlated with Ennking and histological type. Up-regulated BC050642 could promote cell proliferation, induce colony formation and meanwhile inhibit cell apoptosis. Conclusions: BC050642 is up-regulated in osteosarcma and its over-expression promotes tumor development via down-regulating the expression of c-myc. It also may be an independent prognostic biomarker for osteosarcoma.


Assuntos
Neoplasias Ósseas/diagnóstico , Neoplasias Ósseas/patologia , Carcinogênese/genética , Osteossarcoma/diagnóstico , Osteossarcoma/patologia , RNA Longo não Codificante/genética , Adulto , Apoptose/genética , Neoplasias Ósseas/genética , Estudos de Casos e Controles , Linhagem Celular Tumoral , Proliferação de Células/genética , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Masculino , Osteossarcoma/genética , Prognóstico , Proteínas Proto-Oncogênicas c-myc/genética , Análise de Sobrevida
4.
J Assoc Physicians India ; 67(4): 76-78, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31299847

RESUMO

A 21-year old female, recently diagnosed with osteosarcoma of right humerus, presented to the emergency with history of fever, productive cough, chest pain and progressive respiratory distress for six days. Initial investigations suggested pneumonia but she did not respond to parenteral antibiotics. CT pulmonary angiogram revealed bilateral pulmonary artery embolism. Thrombolysis was performed using alteplase, which failed to improve the clinical condition. In view of underlying malignancy, a possibility of tumour-embolism was considered and she was started on chemotherapy for osteosarcoma. There was dramatic improvement in her respiratory symptoms after the first chemotherapy cycle, along with radiological resolution of the embolism. This case highlights the importance of suspecting tumour embolism in a known case of malignancy with respiratory distress.


Assuntos
Antineoplásicos/uso terapêutico , Neoplasias Ósseas/diagnóstico , Osteossarcoma/diagnóstico , Embolia Pulmonar/diagnóstico , Adulto , Angiografia , Neoplasias Ósseas/complicações , Neoplasias Ósseas/tratamento farmacológico , Feminino , Humanos , Osteossarcoma/complicações , Osteossarcoma/tratamento farmacológico , Pneumonia , Embolia Pulmonar/complicações , Embolia Pulmonar/tratamento farmacológico , Adulto Jovem
5.
Medicine (Baltimore) ; 98(26): e16141, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31261538

RESUMO

The aim of this study was to develop nomograms to predict long-term overall survival and cancer-specific survival of patients with osteosarcoma.We carried out univariate and multivariate analyses and set up nomograms predicting survival outcome using osteosarcoma patient data collected from the Surveillance, Epidemiology and End Results (SEER) program of the National Cancer Institute (2004-2011, n = 1426). The patients were divided into a training cohort (2004-2008, n = 863) and a validation cohort (2009-2011, n = 563), and the mean follow-up was 55 months.In the training cohort, 304 patients (35.2%) died from osteosarcoma and 91 (10.5%) died from other causes. In the validation cohort, 155 patients (27.5%) died from osteosarcoma and (12.3%) died from other causes. Nomograms predicting overall survival (OS) and cancer-specific survival (CSS) were developed according to 6 clinicopathologic factors (age, tumor site, historic grade, surgery, AJCC T/N, and M), with concordance indexes (C-index) of 0.725 (OS) and 0.718 (CSS), respectively. The validation C-indexes were 0.775 and 0.742 for OS and CSS, respectively.Our results suggest that we have successfully developed highly accurate nomograms for predicting 5-year OS and CSS for osteosarcoma patients. These nomograms will help surgeons customize treatment and monitoring strategies for osteosarcoma patients.


Assuntos
Nomogramas , Osteossarcoma/diagnóstico , Adolescente , Adulto , Criança , Pré-Escolar , Estudos de Coortes , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Osteossarcoma/mortalidade , Osteossarcoma/patologia , Osteossarcoma/terapia , Programa de SEER , Análise de Sobrevida , Adulto Jovem
6.
World Neurosurg ; 130: 512-515, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31279922

RESUMO

BACKGROUND: Hemangiomas are benign blood vessels tumors that represent less than 1% of all the bone neoplasms. Calvarial hemangiomas are mainly solitary lesions commonly located in the frontal and parietal bone; however, they may occur in any skull region. These tumors increase in size over a period of months to years before they start showing their first symptoms such as headache, bone deformity, and pathological fractures. Differential diagnosis with osteosarcoma should be considered. Surgical resection with a safety margin is a standard treatment of the cranial hemangioma. Furthermore, radiotherapy has proven to stop the tumor's growth but not its size. CASE DESCRIPTION: We treated an 11-year-old male who had a rare case of a capillary hemangioma located in the clivus bone. The patient underwent 2 endoscopic endonasal resection because of tumor recurrence. Surgical safety margins are highly recommended, but this procedure could not be performed because of the tumor's location. After the second relapse, the oncology team decided to initiate radiotherapy. At 6-month follow-up, the tumor reduced its size and remained unchanged. CONCLUSIONS: Surgical safety margins are highly important to prevent recurrence in this type of bone tumors. Skull base hemangiomas are a big challenge when you want to achieve these safety margins. We believe that a combined treatment of surgery and radiotherapy should be considered as the main treatment.


Assuntos
Fossa Craniana Posterior/diagnóstico por imagem , Fossa Craniana Posterior/patologia , Hemangioma/diagnóstico , Hemangioma/patologia , Neoplasias da Base do Crânio/diagnóstico , Neoplasias da Base do Crânio/patologia , Criança , Fossa Craniana Posterior/irrigação sanguínea , Diagnóstico Diferencial , Humanos , Masculino , Osteossarcoma/diagnóstico , Osteossarcoma/patologia , Neoplasias da Base do Crânio/irrigação sanguínea
7.
Anticancer Res ; 39(6): 3159-3165, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31177162

RESUMO

BACKGROUND: Patients with sarcoma are particularly vulnerable to psychosocial distress. The aim of this study was to collect preliminary data on the prevalence of psychosocial distress in such patients during follow-up care and identify risk factors associated with higher psycho-oncological stress levels. PATIENTS AND METHODS: The study retrospectively enrolled 202 patients with bone or soft-tissue sarcomas who underwent routine psychosocial distress screening during their follow-up care. All patients were screened using an electronic cancer-specific questionnaire. RESULTS: Females and patients who underwent radiotherapy were more distressed. Psychosocial distress levels were markedly higher in the early postoperative phase, but approximately one-third of patients showed high psychosocial distress levels even more than 2 years postoperatively. CONCLUSION: The results underscore the importance of routine psychosocial distress screenings in patients with sarcoma, which should be performed throughout the follow-up period.


Assuntos
Assistência ao Convalescente/psicologia , Neoplasias Ósseas/terapia , Saúde Mental , Osteossarcoma/terapia , Sarcoma/terapia , Neoplasias de Tecidos Moles/terapia , Estresse Psicológico/epidemiologia , Estresse Psicológico/psicologia , Inquéritos e Questionários , Adaptação Psicológica , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Neoplasias Ósseas/diagnóstico , Neoplasias Ósseas/psicologia , Efeitos Psicossociais da Doença , Feminino , Alemanha/epidemiologia , Humanos , Masculino , Pessoa de Meia-Idade , Osteossarcoma/diagnóstico , Osteossarcoma/psicologia , Valor Preditivo dos Testes , Dados Preliminares , Prevalência , Estudos Retrospectivos , Fatores de Risco , Sarcoma/diagnóstico , Sarcoma/psicologia , Neoplasias de Tecidos Moles/diagnóstico , Neoplasias de Tecidos Moles/psicologia , Estresse Psicológico/diagnóstico , Fatores de Tempo , Adulto Jovem
8.
J Biol Regul Homeost Agents ; 33(2 Suppl. 1): 39-43, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31169001

RESUMO

With the advent of the molecularly targeted therapies, identifying molecular therapeutic targets and molecolar marker is increasingly important, especially in neoplastic diseases. Several studies show VEGF is involved in neo-angiogenesis in many solid cancers, as breast, lung, renal, gastric carcinomas, through promoting endothelial cell growth and migration. Conversely the relationship between VEFG and tumours of the musculoskeletal system is yet unclear, in particular the role of VEGF has not yet been completely understood in these tumours. Chondrosarcoma, Ewing's Sarcoma and Osteosarcoma are the tumours of the musculoskeletal system in which the activity of VEGF has been closely studied. The present study aims to give an overview focused on the relationship between VEGF and these three cancers.


Assuntos
Neoplasias Ósseas/diagnóstico , Condrossarcoma/diagnóstico , Osteossarcoma/diagnóstico , Sarcoma de Ewing/diagnóstico , Fator A de Crescimento do Endotélio Vascular/sangue , Biomarcadores Tumorais/sangue , Humanos , Prognóstico
9.
Intern Med ; 58(17): 2555-2560, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31178507

RESUMO

A 73-year-old man was referred to our hospital with a persistent fever, anemia, and a mass in the left pubic region. The findings of biopsy evaluations of the mass and a left inguinal lymph node were consistent with Castleman disease (CD) of plasma cell type. His serum interleukin 6 (IL-6) level was remarkably elevated, supporting the diagnosis of CD. However, imaging analyses revealed destruction of the pubic bone by the mass, which was atypical for CD. Therefore, another deeper biopsy was performed, which finally led to the diagnosis of IL-6-producing osteosarcoma. We conclude that clinicians should carefully exclude malignancies prior to making a CD diagnosis.


Assuntos
Neoplasias Ósseas/diagnóstico , Hiperplasia do Linfonodo Gigante/diagnóstico , Osteossarcoma/diagnóstico , Osso Púbico/patologia , Idoso , Anemia/etiologia , Biópsia , Neoplasias Ósseas/diagnóstico por imagem , Neoplasias Ósseas/patologia , Hiperplasia do Linfonodo Gigante/patologia , Diagnóstico Diferencial , Febre/etiologia , Humanos , Inflamação/patologia , Interleucina-6/sangue , Linfonodos/patologia , Masculino , Osteossarcoma/diagnóstico por imagem , Osteossarcoma/patologia , Plasmócitos/patologia , Osso Púbico/diagnóstico por imagem
10.
Rev. Asoc. Argent. Ortop. Traumatol ; 84(2): 149-154, jun. 2019.
Artigo em Espanhol | LILACS | ID: biblio-1003024

RESUMO

El osteosarcoma "tipo osteoblastoma", una variante menos agresiva del osteosarcoma, es una enfermedad poco frecuente y representa un desafío diagnóstico tanto clínico como histopatológico. Se han publicado escasos reportes de casos de este tumor. Presentamos a un paciente de 16 años con un osteosarcoma "tipo osteoblastoma" localizado en la columna cervical. La biopsia ósea bajo tomografía reveló una lesión con un patrón de crecimiento permeativo, con sospecha de osteosarcoma "tipo osteoblastoma". Se realizó una espondilectomía total en bloque mediante un doble abordaje. Se describen el caso clínico, la secuencia diagnóstica, la técnica quirúrgica y el seguimiento a 10 años. Nivel de Evidencia: IV


Osteoblastoma-like osteosarcoma, a less aggressive variant of osteosarcoma, is a rare tumor which presents a clinical and histopathological diagnostic challenge. We have found few reports of cases of this tumor in the literature. In this case, we describe an osteoblastoma-like osteosarcoma of the cervical spine in a 16-year-old patient. CT-guided bone biopsy showed a lesion with a permeated growth pattern, which led to the suspicion of an osteoblastoma-like osteosarcoma. A total en bloc spondylectomy was performed through a dual approach. We present the clinical case, the diagnostic sequence, the surgical technique and 10-year follow-up results. Level of Evidence: IV


Assuntos
Adolescente , Neoplasias Ósseas/cirurgia , Osteossarcoma/cirurgia , Osteossarcoma/diagnóstico , Vértebras Cervicais/patologia , Osteoblastoma/cirurgia , Osteoblastoma/diagnóstico
11.
Int J Clin Oncol ; 24(8): 976-982, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31111286

RESUMO

BACKGROUND: Osteosarcoma (OS) is the most common malignant bone tumor in young adults and adolescents with approximately 3 million new cases annually. Due to the lack of sensitive and specific diagnostic biomarkers, although OS patients are curable after surgical resection, many patients suffer from metastasis or recurrence. This study aimed to investigate whether circulating microRNAs (miRNAs) could serve as biomarkers for the diagnosis of OS. MATERIALS AND METHODS: Healthy individuals and OS patients enrolled in this study came from Nanjing First Hospital. First, candidate miRNAs were selected by integrated analysis of two GEO datasets and a publicly available miRNA dataset. The expression of these miRNAs in tissues and serum samples were subsequently examined through qRT-PCR. The diagnostic utility of these differential miRNAs was examined by using receiver operating characteristic (ROC) curve analysis. Finally, the potential signaling pathways associated with candidate miRNAs were searched through online tools. RESULTS: Four miRNAs (miR-487a, miR-493-5p, miR-501-3p and miR-502-5p) were selected to further investigate their diagnostic potential for OS. We discovered miR-487a, miR-493-5p, miR-501-3p and miR-502-5p were upregulated in OS tissues and serums. Besides, miR-487a, miR-493-5p, miR-501-3p and miR-502-5p in peripheral blood of OS patients were tumor-derived. The area under the ROC curve (AUC) was 0.83 (95% CI 0.71-0.97) for miR-487a, 0.79 (95% CI 0.66-0.93) for miR-493-5p, 0.82 (95% CI 0.68-0.95) for miR-501-3p, 0.83 (95% CI 0.72-0.95) for miR-502-5p, and 0.89 (95% CI 0.78-1.0) for miRNAs combination. CONCLUSION: Circulating miR-487a, miR-493-5p, miR-501-3p and miR-502-5p were novel potential diagnostic biomarkers of OS.


Assuntos
Biomarcadores Tumorais/genética , Neoplasias Ósseas/diagnóstico , MicroRNAs/genética , Osteossarcoma/diagnóstico , Adolescente , Biomarcadores Tumorais/sangue , Neoplasias Ósseas/sangue , Neoplasias Ósseas/genética , Estudos de Casos e Controles , Feminino , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Humanos , Masculino , MicroRNAs/sangue , Osteossarcoma/sangue , Osteossarcoma/genética , Prognóstico , Curva ROC
12.
J Med Case Rep ; 13(1): 136, 2019 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-31068214

RESUMO

BACKGROUND: Metachronous osteosarcoma is a rare form of osteosarcoma. The occurrence of metachronous tumor in soft tissue is exceedingly rare. The pathogenesis of metachronous osteosarcoma, as to whether it represents multiple true primaries or metastatic disease, is still obscure. CASE PRESENTATION: A 49-year-old Indian man presented with progressively increasing swelling in his left hand of 2 months' duration. An X-ray showed a soft tissue lesion. Contrast-enhanced computed tomography showed a soft tissue mass lesion, with peripheral enhancement and central necrotic areas in radial palmar soft tissue overlying second metacarpophalangeal region with no obvious bony osteolysis. Possibilities of acute abscess, resolving hematoma, or aggressive soft tissue mass lesion were suggested. An incision biopsy showed morphological features of epithelioid osteosarcoma, which was confirmed on immunohistochemistry. A detailed history revealed that our patient was diagnosed as having osteosarcoma of his right leg 3 years previously. Based on history, radiology, morphology, and immunohistochemistry, a final diagnosis of extraskeletal (soft tissue) epithelioid osteogenic sarcoma of the left hand occurring as a metachronous tumor 3 years after diagnosis of primary osteosarcoma in the right leg was given. CONCLUSION: This is probably the first reported case of extraskeletal (soft tissue) epithelioid osteosarcoma occurring as a metachronous tumor 3 years after diagnosis of primary osteosarcoma in the right leg. The prognosis of metachronous skeletal osteosarcoma is poor as compared to that of relapse limited to lungs. In late metachronous osteosarcoma, combined-modality therapy comprising surgery and aggressive chemotherapy may affect long-term survival. Lifelong follow-up of surviving patients with osteosarcoma is necessary and if metachronous osteosarcoma is discovered, it should be treated with curative intent.


Assuntos
Segunda Neoplasia Primária/patologia , Osteossarcoma/patologia , Neoplasias de Tecidos Moles/patologia , Antibióticos Antineoplásicos/uso terapêutico , Protocolos de Quimioterapia Combinada Antineoplásica , Cisplatino/uso terapêutico , Terapia Combinada , Doxorrubicina/uso terapêutico , Mãos/cirurgia , Humanos , Masculino , Pessoa de Meia-Idade , Segunda Neoplasia Primária/diagnóstico , Segunda Neoplasia Primária/diagnóstico por imagem , Segunda Neoplasia Primária/terapia , Osteossarcoma/diagnóstico , Osteossarcoma/diagnóstico por imagem , Osteossarcoma/terapia , Neoplasias de Tecidos Moles/diagnóstico , Neoplasias de Tecidos Moles/diagnóstico por imagem , Neoplasias de Tecidos Moles/terapia , Tomografia Computadorizada por Raios X
13.
Ir J Med Sci ; 188(4): 1163-1167, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31054046

RESUMO

BACKGROUND: The overall survival of patients with localised osteosarcoma has dramatically improved with the introduction of multidrug chemotherapeutic regimens into the treatment paradigm. However, despite optimal treatment, all-cause mortality remains higher among osteosarcoma survivors than in the general population. The development of second malignant neoplasms contributes to this higher mortality rate. CASE SERIES: We present three cases of patients definitively treated for osteosarcoma who subsequently developed a second malignant neoplasm. The first case describes a 17-year-old female with osteosarcoma of her right femur treated with surgical resection and perioperative chemotherapy. Ten years later, she was diagnosed with metastatic HER2-positive breast cancer. Genetic testing identified a germline TP53 mutation, confirming the presence of Li-Fraumeni syndrome. The second case details an 18-year-old male with osteosarcoma of his right humerus treated with definitive resection and perioperative chemotherapy. He was diagnosed with appendiceal adenocarcinoma after presenting with acute abdominal pain 17 years later. The third case reviewed is of a 36-year-old male with osteosarcoma of his right femur treated with definitive resection and adjuvant chemotherapy. A diagnosis of leiomyosarcoma was made 7 years later following surveillance imaging. DISCUSSION: The risk of second malignant neoplasms in osteosarcoma may relate to previous oncological treatment, an inherited cancer predisposition syndrome or a spontaneous new neoplasm. Although screening for a second malignancy is not routinely recommended for osteosarcoma survivors, a high degree of clinical suspicion should be maintained during surveillance.


Assuntos
Adenocarcinoma/diagnóstico , Neoplasias Ósseas/diagnóstico , Osteossarcoma/diagnóstico , Adolescente , Adulto , Neoplasias da Mama/diagnóstico , Quimioterapia Adjuvante , Feminino , Fêmur/patologia , Humanos , Leiomiossarcoma/diagnóstico , Síndrome de Li-Fraumeni/diagnóstico , Masculino , Segunda Neoplasia Primária/patologia
14.
J Oral Pathol Med ; 48(6): 441-450, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31062892

RESUMO

The purpose of this study was to perform a systematic review of the literature concerning all documented cases of malignant transformation of craniomaxillofacial fibro-osseous lesions (FOLs). Three electronic databases were searched. Data were evaluated descriptively. Kaplan-Meier survival curves were constructed and compared using the log-rank test. A critical appraisal of included articles was performed through the Joanna Briggs Institute tool. A total of 19 studies including 27 patients were selected for data extraction. Twenty-six cases were initially diagnosed as fibrous dysplasia and one as ossifying fibroma. The mean age at the time of malignant transformation was 38.11 years, and the average time from initial diagnosis to malignant transformation was 18.2 years. The male:female ratio was 1:1.2, and the maxilla:mandible ratio was 1.5:1. The histological type of the malignant tumor was predominantly osteosarcoma. Follow-up was available for 21 patients. The 3-year overall survival rate was 51%. Mandible tumors and diagnoses other than osteosarcoma tended to have poor survival rates, but no significant difference was identified. We concluded that between all FOLs, only fibrous dysplasia seems to have a considerable increased risk of malignant transformation. Thus, a regular and long follow-up period is advised.


Assuntos
Fibroma Ossificante/patologia , Displasia Fibrosa Óssea/patologia , Neoplasias Mandibulares/diagnóstico , Osteossarcoma/diagnóstico , Humanos , Taxa de Sobrevida
15.
Bioengineered ; 10(1): 133-141, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31055998

RESUMO

OBJECTIVE: S100A9 is a calcium- and zinc-binding molecule of S100 family. The aim of the study was to evaluate the role of S100A9 in osteosarcoma (OS) and its value as a diagnostic and therapeutic target in OS. METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR), immunohistochemistry and microdissection-based mRNA analysis were used to detect S100A9 mRNA and protein expression in OS and normal bone tissues and its potential as a diagnostic marker in OS. In vitro experiments with RNA interference were performed to evaluate the functional role of S100A9 and its potential as a therapeutic target in OS. RESULTS: S100A9 mRNA levels were significantly higher in OS tissues than that of in normal bone tissues. Receiver operating characteristic curves showed that S100A9 could be a useful diagnostic marker in OS. In vitro data showed that inhibition of S100A9 decreased the proliferation and invasiveness of OS cells, and these findings were supported by microarray data. CONCLUSIONS: Assessment of S100A9 mRNA expression is a promising tool for the diagnosis of OS, and S100A9 may be a promising therapeutic target in OS.


Assuntos
Biomarcadores Tumorais/genética , Neoplasias Ósseas/diagnóstico , Calgranulina B/genética , Regulação Neoplásica da Expressão Gênica , Osteossarcoma/diagnóstico , RNA Mensageiro/genética , Biomarcadores Tumorais/metabolismo , Neoplasias Ósseas/genética , Neoplasias Ósseas/metabolismo , Neoplasias Ósseas/patologia , Calgranulina B/metabolismo , Linhagem Celular Tumoral , Proliferação de Células , Progressão da Doença , Perfilação da Expressão Gênica , Humanos , Metástase Linfática , Invasividade Neoplásica , Proteínas de Neoplasias , Osteoblastos/metabolismo , Osteoblastos/patologia , Osteossarcoma/genética , Osteossarcoma/metabolismo , Osteossarcoma/patologia , RNA Mensageiro/antagonistas & inibidores , RNA Mensageiro/metabolismo , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , Curva ROC , Transdução de Sinais
16.
Cancer Biomark ; 25(1): 1-10, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31006663

RESUMO

BACKGROUND: Despite the major advances in the treatment, the overall survival of osteosarcoma remains poor. MicroRNAs (miRNAs) are involved in tumorigenesis and progression though modulating their target genes. In the present study, the roles of miR-1285-3p in osteosarcoma was investigated. METHODS: Microarray profiling was applied to distinguish the up and down regulated microRNAs in osteosarcoma. Quantitative real-time PCR (qRT-PCR) assay was performed to detect the expression of miR-1285-3p and YAP1 expression. MTT and transwell assays were carried out to determine the cells proliferation and invasion respectively. Moreover, dual luciferase reporter assay was performed to evaluate the binding efficiency between miR-1285-3p and the 3'UTR of YAP1. RESULTS: MiR-1285-3p was down regulated in osteosarcoma tissues and cell lines and the reduction of miR-1285-3p expression predicted a poor overall survival of osteosarcoma patients. Ectopic expression of miR-1285-3p inhibited osteosarcoma cell proliferation, colony formation and invasion. In addition, YAP1 was further demonstrated as a direct target of miR-1285-3p. Moreover, overexpression of YAP1 reversed the inhibitory effects of miR-1285-3p on osteosarcoma cells proliferation and invasion. CONCLUSIONS: MiR-1285-3p which was low expressed in osteosarcoma inhibited the proliferation and invasion of osteosarcoma cells via direct targeting YAP1. These results suggested that miR-1285-3p might be a potential therapeutic targets and biomarker in osteosarcoma.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/genética , Biomarcadores Tumorais/metabolismo , Neoplasias Ósseas/diagnóstico , Neoplasias Ósseas/metabolismo , MicroRNAs/metabolismo , Osteossarcoma/diagnóstico , Osteossarcoma/metabolismo , Fosfoproteínas/genética , Regiões 3' não Traduzidas , Biomarcadores Tumorais/genética , Neoplasias Ósseas/genética , Neoplasias Ósseas/patologia , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Regulação para Baixo/genética , Feminino , Expressão Gênica , Humanos , Masculino , MicroRNAs/genética , Invasividade Neoplásica , Osteossarcoma/genética , Osteossarcoma/patologia , Prognóstico , Ligação Proteica , Taxa de Sobrevida , Fatores de Transcrição , Adulto Jovem
17.
Cesk Patol ; 55(1): 42-47, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30939886

RESUMO

35-year-old woman suffered prolonged pain in the left shoulder, where an aggressively growing tumor of the proximal humerus was revealed thereafter. The lesion caused massive osteolysis of the metaepiphysis with cortical disruption, but no soft tissue extension was evident. Given the unsatisfactory effect, the ongoing neoadjuvant chemotherapy was prematurely ceased and the resection 13 cm long segment of bone with modular prosthesis replacement followed. Histologically, clear-cut malignant tumor with both the presence of numerous reactive osteoclast-like giant cells and geographic structural deposition of chondroid matrix bore a close resemblance to chondroblastoma. Dominant cellular composition formed solid mosaic clusters of large, atypical, frequently binucleated cells with voluminous eosinophilic cytoplasm. Impressive nuclear pleomorphism was accentuated by both the grooving and atypical mitotic figures. Thorough sampling disclosed limited, but sharply contrasting parts, where biphasic arrangement of small uniform stromal elements together with regularly distributed, reactive osteoclasts suggested putative precursor giant cell lesion. Except the osteoclasts, all matrical and stromal cells were strongly SOX9 and D2-40 positive; in contrary desmin, SATB2, S100 and p63 yielded completely negative results. Detected H3F3A c.103G>T mutation in exon 2 finally established true nature of that peculiar neoplastic proliferation and lead to descriptive term of primary chondroblastoma-like malignant giant cell tumor. In the setting of all the microscopic variability, histogenesis and complex differential diagnosis of skeletal (malignant) giant cell lesions, there are discussed e.g. aggressive/malignant chondroblastoma, chondroblastoma-like osteosarcoma or giant cell-rich osteosarcoma and practical impact of specific mutational analysis results as well.


Assuntos
Neoplasias Ósseas , Condroblastoma , Tumores de Células Gigantes , Proteínas de Ligação à Região de Interação com a Matriz , Osteossarcoma , Adulto , Neoplasias Ósseas/complicações , Neoplasias Ósseas/diagnóstico , Neoplasias Ósseas/cirurgia , Condroblastoma/complicações , Condroblastoma/diagnóstico , Condroblastoma/cirurgia , Feminino , Tumores de Células Gigantes/complicações , Tumores de Células Gigantes/diagnóstico , Tumores de Células Gigantes/cirurgia , Humanos , Úmero , Osteossarcoma/complicações , Osteossarcoma/diagnóstico , Osteossarcoma/cirurgia , Fatores de Transcrição
18.
Korean J Radiol ; 20(5): 801-811, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30993931

RESUMO

OBJECTIVE: To determine whether diffusion kurtosis imaging (DKI) is effective in monitoring tumor response to neoadjuvant chemotherapy in patients with osteosarcoma. MATERIALS AND METHODS: Twenty-nine osteosarcoma patients (20 men and 9 women; mean age, 17.6 ± 7.8 years) who had undergone magnetic resonance imaging (MRI) and DKI before and after neoadjuvant chemotherapy were included. Tumor volume, apparent diffusion coefficient (ADC), mean diffusivity (MD), mean kurtosis (MK), and change ratio (ΔX) between pre- and post-treatment were calculated. Based on histologic response, the patients were divided into those with good response (≥ 90% necrosis, n = 12) and those with poor response (< 90% necrosis, n = 17). Several MRI parameters between the groups were compared using Student's t test. The correlation between image indexes and tumor necrosis was determined using Pearson's correlation, and diagnostic performance was compared using receiver operating characteristic curves. RESULTS: In good responders, MDpost, ADCpost, and MKpost values were significantly higher than in poor responders (p < 0.001, p < 0.001, and p = 0.042, respectively). The ΔMD and ΔADC were also significantly higher in good responders than in poor responders (p < 0.001 and p = 0.01, respectively). However, no significant difference was observed in ΔMK (p = 0.092). MDpost and ΔMD showed high correlations with tumor necrosis rate (r = 0.669 and r = 0.622, respectively), and MDpost had higher diagnostic performance than ADCpost (p = 0.037) and MKpost (p = 0.011). Similarly, ΔMD also showed higher diagnostic performance than ΔADC (p = 0.033) and ΔMK (p = 0.037). CONCLUSION: MD is a promising biomarker for monitoring tumor response to preoperative chemotherapy in patients with osteosarcoma.


Assuntos
Neoplasias Ósseas/terapia , Imagem de Difusão por Ressonância Magnética , Terapia Neoadjuvante , Osteossarcoma/terapia , Adolescente , Adulto , Área Sob a Curva , Neoplasias Ósseas/diagnóstico , Neoplasias Ósseas/diagnóstico por imagem , Criança , Feminino , Humanos , Processamento de Imagem Assistida por Computador/métodos , Masculino , Necrose , Osteossarcoma/diagnóstico , Osteossarcoma/diagnóstico por imagem , Estudos Prospectivos , Curva ROC , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Adulto Jovem
19.
PLoS One ; 14(4): e0210706, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30995247

RESUMO

Pathological estimation of tumor necrosis after chemotherapy is essential for patients with osteosarcoma. This study reports the first fully automated tool to assess viable and necrotic tumor in osteosarcoma, employing advances in histopathology digitization and automated learning. We selected 40 digitized whole slide images representing the heterogeneity of osteosarcoma and chemotherapy response. With the goal of labeling the diverse regions of the digitized tissue into viable tumor, necrotic tumor, and non-tumor, we trained 13 machine-learning models and selected the top performing one (a Support Vector Machine) based on reported accuracy. We also developed a deep-learning architecture and trained it on the same data set. We computed the receiver-operator characteristic for discrimination of non-tumor from tumor followed by conditional discrimination of necrotic from viable tumor and found our models performing exceptionally well. We then used the trained models to identify regions of interest on image-tiles generated from test whole slide images. The classification output is visualized as a tumor-prediction map, displaying the extent of viable and necrotic tumor in the slide image. Thus, we lay the foundation for a complete tumor assessment pipeline from original histology images to tumor-prediction map generation. The proposed pipeline can also be adopted for other types of tumor.


Assuntos
Neoplasias Ósseas/diagnóstico , Aprendizado Profundo , Interpretação de Imagem Assistida por Computador/métodos , Osteossarcoma/diagnóstico , Máquina de Vetores de Suporte , Neoplasias Ósseas/patologia , Osso e Ossos/patologia , Conjuntos de Dados como Assunto , Humanos , Necrose/patologia , Osteossarcoma/patologia , Curva ROC , Reprodutibilidade dos Testes , Software
20.
J Cutan Pathol ; 46(7): 546-549, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30972791

RESUMO

Extraskeletal osteosarcoma (ESOS) is a rare variant of osteosarcoma that arises without attachment to the underlying skeleton. These cancers are typically found embedded in deeper tissues, most commonly the muscle or fascia, and are rarely found within the skin or subcutis. Most tumors are large in size upon initial presentation, and carry a poor prognosis. We discuss the case of a 48-year-old Caucasian woman who presented to a dermatology clinic with an asymptomatic, small, mobile, subcutaneous mass that appeared clinically benign. After elective removal and histopathologic examination, the patient was diagnosed with ESOS. ESOS presenting in this manner is exceedingly rare, and this case highlights the importance of sending all excised specimens, even those with a benign presentation, for pathologic examination.


Assuntos
Osteossarcoma , Neoplasias Cutâneas , Tela Subcutânea , Feminino , Humanos , Pessoa de Meia-Idade , Osteossarcoma/diagnóstico , Osteossarcoma/metabolismo , Osteossarcoma/patologia , Neoplasias Cutâneas/diagnóstico , Neoplasias Cutâneas/metabolismo , Neoplasias Cutâneas/patologia , Tela Subcutânea/metabolismo , Tela Subcutânea/patologia
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