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1.
Food Chem ; 303: 125346, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31446364

RESUMO

The effect of a respiratory quotient dynamic controlled atmosphere (DCA - RQ), which induces ethanol production through low oxygen storage, and ethanol application on softening of Braeburn apples stored at different temperatures was investigated. DCA - RQ storage was associated with the activation of the alcohol dehydrogenase (ADH) and greater anaerobic metabolism in comparison with DCA - CF (chlorophyll fluorescence) and controlled atmosphere (CA) storage. Greater anaerobic metabolism resulted in lower ethylene production, ACC oxidase activity, membrane permeability, -galactosidase activity and, therefore, less softening after long-term storage. Ethanol application after CA storage decreased ethylene biosynthesis, respiration rate and membrane permeability. Storage at 3 °C resulted in the lowest soluble solids and acidity, but not softening. In conclusion, Braeburn apples could be stored at 3 °C under DCA - RQ1.5. Additionally, ethanol produced by the fruit, or applied externally, had a significant effect on inhibiting softening during and after storage.


Assuntos
Atmosfera , Etanol/farmacologia , Malus/efeitos dos fármacos , Malus/metabolismo , Oxigênio/metabolismo , Anaerobiose/efeitos dos fármacos , Frutas/efeitos dos fármacos , Frutas/metabolismo
2.
Rev. Esc. Enferm. USP ; 53: e03469, Jan.-Dez. 2019. tab, graf
Artigo em Inglês, Português | LILACS, BDENF - Enfermagem | ID: biblio-1020388

RESUMO

RESUMO Objetivo Avaliar modificações cardiorrespiratórias em decorrência de sessão única de oxigenoterapia hiperbárica. Método Estudo aleatorizado com pacientes: grupos-controle e oxigenoterapia hiperbárica. As avaliações ocorreram no início, durante e após a exposição ao oxigênio puro acima de uma atmosfera, durante 2 horas. A pressão arterial sistêmica, saturação periférica de oxigênio, frequência de pulso, volume e capacidade pulmonar, pressões inspiratória e expiratória máximas foram avaliadas. A saturação periférica de oxigênio, frequência de pulso e pressão arterial sistêmica foram avaliadas durante a pressurização na primeira hora. Os dados foram avaliados pelo teste de ANOVA, Mann-Whitney e teste t independente (p<0,05). Resultados Foram avaliados 14 pacientes adultos. No grupo sob terapia (sete sujeitos), idade: 49,57±14,59 anos houve redução da frequência de pulso de 16 batimentos por minuto após 35 minutos de terapia (análise intragrupo), e a saturação periférica de oxigênio foi maior neste mesmo período se comparado ao grupo-controle. Conclusão A oxigenoterapia hiperbárica promove alterações cardiorrespiratórias com o aumento da saturação periférica de oxigênio e redução da frequência de pulso, sem alterar os níveis pressóricos arteriais e a força, volumes e capacidades respiratórios.


RESUMEN Objetivo Evaluar modificaciones cardiorrespiratorias consecuentes de sesión única de oxigenoterapia hiperbárica. Método Estudio aleatorizado con pacientes: grupos de control y oxigenoterapia hiperbárica. Las evaluaciones ocurrieron en el inicio, durante y después de la exposición al oxígeno puro por encima de una atmósfera, durante dos horas. La presión arterial sistémica, saturación periférica de oxígeno, frecuencia de pulso, volumen y capacidad pulmonar, presiones inspiratoria y espiratoria máximas fueron evaluadas. La saturación periférica de oxígeno, frecuencia de pulso y presión arterial sistémica fueron evaluadas durante la presurización en la primera hora. Los datos fueron evaluados por el test de ANOVA, Mann Whitney y prueba t independiente (p<0,05). Resultados Fueron evaluados 14 pacientes adultos. En el grupo bajo terapia (siete sujetos), edad: 49,57±14,59 años, hubo reducción de la frecuencia de pulso de 16 latidos por minuto tras 35 minutos de terapia (análisis intragrupo), y la saturación periférica de oxígeno fue mayor en ese mismo período si comparado con el grupo de control. Conclusión La oxigenoterapia hiperbárica proporciona alteraciones cardiorrespiratorias con el aumento de la saturación periférica de oxígeno y la reducción de la frecuencia de pulso, sin alterar los niveles de presión arteriales y la fuerza, volúmenes y capacidades respiratorios.


ABSTRACT Objective To evaluate cardiorespiratory alterations due to a single session of hyperbaric oxygen therapy. Method Randomized study with patients: a control group and hyperbaric oxygen therapy. Evaluations occurred in the beginning, during, and after exposure to pure oxygen above atmosphere for 2 hours. Systemic blood pressure, peripheral oxygen saturation, pulse rate, lung volume and lung capacity, and maximal inspiratory and expiratory pressures were evaluated. Peripheral oxygen saturation, pulse rate, and systemic blood pressure were evaluated during the pressurizing in the first hour. Data were evaluated by means of ANOVA, Mann-Whitney, and independent t-test (p<0.05). Results A total of 14 adult patients were evaluated. In the group under therapy (seven subjects), aged: 49.57±14.59 years, there was a decrease in the pulse rate of 16 beats per minute after 35 minutes of therapy (intragroup analysis), and the peripheral oxygen saturation was higher within the same period compared to the control group. Conclusion The hyperbaric oxygen therapy promotes cardiorespiratory alterations with the increase of the peripheral oxygen saturation and decrease of the pulse rate, without altering blood pressure levels and the strength, volumes, and respiratory capacities.


Assuntos
Humanos , Masculino , Feminino , Oxigenoterapia/enfermagem , Pressão Arterial , Oxigenação Hiperbárica/enfermagem , Oxigênio
3.
Isr Med Assoc J ; 21(11): 743-746, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31713363

RESUMO

BACKGROUND: The use of oral midazolam as premedication to induce anxiolysis before surgical procedures under local anesthesia is widely accepted in plastic surgery. Rhinoplasty performed under local anesthesia is known to generate high levels of perioperative anxiety, thus the use of appropriate premedication is important. Oral midazolam has been shown to be safe in various procedures. However, the safety of oral midazolam before rhinoplasty has not been evaluated. OBJECTIVES: To evaluate the safety of premedication with oral midazolam prior to rhinoplasty by analyzing the intraoperative blood oxygen saturation levels as predictors of adverse respiratory events. METHODS: We retrospectively reviewed the anesthesia records of 62 patients who underwent rhinoplasty under local anesthesia and received premedication with oral midazolam for anxiolysis between March 2017 and December 2017. The median age of the patients was 25.4 years, and they were all classified as American Society of Anesthesiologists class 1. The patients received 10 mg midazolam hydrochloride orally 1 hour prior to the procedure. Oxygen blood saturation was monitored using a pulse oximeter and recorded every 15 minutes. RESULTS: All the patients maintained blood oxygen saturation levels above 95% (median peripheral capillary oxygen saturation 99%) on room air, and they did not require supplemental intraoperative oxygen. There were no transient hypoxemic events during and following the procedure. CONCLUSIONS: Our study confirmed the safety of oral midazolam premedication to reduce perioperative anxiety when performing rhinoplasty under local anesthesia.


Assuntos
Hipnóticos e Sedativos/administração & dosagem , Midazolam/administração & dosagem , Oxigênio/sangue , Rinoplastia , Administração Oral , Adolescente , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Oximetria , Pré-Medicação , Estudos Retrospectivos
4.
Zhongguo Ying Yong Sheng Li Xue Za Zhi ; 35(4): 317-321, 2019 Jul 28.
Artigo em Chinês | MEDLINE | ID: mdl-31701714

RESUMO

OBJECTIVE: To apply hypoxia of different oxygen concentration on C2C12 cells to study the changes of Nrf2 antioxidant system under H2O2. METHODS: The perfect simulative effect time and concentration of H2O2 were chosen. Cell vitality was tested after C2C12 cells cultured in 0.1 mmol/L, 0.25 mmol/L, 0.5 mmol/L, 0.75 mmol/L, 1 mmol/L and 2 mmol/L H2O2 for 1 or 2 h respectively. The C2C12 cells were divided into different oxygen concentration group: 21%O2, 12%O2, 8%O2, 5%O2 respectively. And then cells were treated with H2O2 for 1 h, and collected for determination. Immunofluorescence of Nrf2 and the protein expression of Nrf2 were detected. The expressions of antioxidant enzymes superoxide dismutase 1 (SOD1), superoxide dismutase 2 (SOD2), catalase(CAT), NADPH quinine oxidoreductase-1 (NQO-1), glutathione peroxidase-1 (GPX-1), Heme oxygenase-1 (HO-1) mRNA and cellular ROS levels were tested by high quality fluorescence assay. RESULTS: 0.5 mmol/L H2O2 for 1 h was selected as the conditions of H2O2stimulation. Compared with 21% O2 group, the expressions of Nrf2 mRNA and protein, antioxidant enzymes SOD1, SOD2, CAT, HO-1, NQO-1, GPX-1 mRNA were increased significantly (P<0.05 or P<0.01), and ROS level was lower (P<0.01) in 12%O2 group cells; only the expression of GPX-1 mRNA was increased (P<0.05) in 8%O2 group; the expressions of Nrf2 mRNA and protein expression, antioxidant enzymes SOD1, SOD2, NQO-1, GPX-1 mRNA were decreased significantly(P<0.05 or P<0.01), and ROS level was higher (P<0.01) in 5%O2 group. CONCLUSION: Hypoxia can affect the Nrf2 antioxidant system, and the different oxygen concentrations have different impact. In addition, 12% O2 for 12 h could promote the Nrf2 antioxidant system, and 5% extremely low oxygen may inhibit it.


Assuntos
Antioxidantes/metabolismo , Mioblastos/enzimologia , Fator 2 Relacionado a NF-E2/metabolismo , Estresse Oxidativo , Animais , Linhagem Celular , Sobrevivência Celular , Peróxido de Hidrogênio , Camundongos , Oxigênio , Espécies Reativas de Oxigênio/metabolismo
5.
Anaesthesia ; 74(12): 1620, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31681990
7.
Klin Lab Diagn ; 64(10): 594-598, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31742951

RESUMO

Degenerative-dystrophic processes in the articular tissues are accompanied by the changes in the values of congenital immunity, being a result of immune responses to aseptic inflammation. The purpose of this study - to investigate the oxygen-dependent mechanisms of neutrophil phagocytic activity (NBT-test, myeloperoxidase activity), the biochemical values of lipid peroxidation, the oxidative modification of proteins and the activity of enzyme antioxidant protection in children (at the age of 12-16 years) with osteochondropathy of the femoral head.The increase in spontaneous NBT-test and myeloperoxidase content in neutrophils, the decrease in NBT stimulation index, as well as the statistically significant increase in the activity of superoxide dismutase, the main producer of hydroperoxide, was revealed in the group examined. At the same time, no increase in catalase activity and in the content of lipoperoxidation products was observed, however, redistribution of the products of oxidative modification of proteins with ketone predominance was found. In case of osteochondropathy development in children the activation of the oxygendependent mechanisms of neutrophil phagocytic activity is observed, as well as the increase in the number of the secondary products of protein peroxidation - ketones. An increase in SOD activity, with a simultaneous and significant decrease in catalase activity evidence of accumulation of hydroperoxide causing neutrophil activation and chemotaxis. The study data can be used for predicting osteonecrosis development as additional criteria when decision-making of the advisability of performing reconstructive surgeries of the joint.


Assuntos
Cabeça do Fêmur/patologia , Osteocondrodisplasias/fisiopatologia , Estresse Oxidativo , Oxigênio , Adolescente , Antioxidantes/metabolismo , Catalase/metabolismo , Criança , Humanos , Peroxidação de Lipídeos , Neutrófilos/enzimologia , Peroxidase/metabolismo , Superóxido Dismutase/metabolismo
9.
Rinsho Ketsueki ; 60(9): 1084-1091, 2019.
Artigo em Japonês | MEDLINE | ID: mdl-31597831

RESUMO

The blood supply system for transfusions in Japan functions well. However, in cases of sudden hemorrhagic shock, the swift supply of red blood cell (RBC) product might be difficult, particularly when medical care is required in remote regions and in obstetric medicine, where there is always a risk of hemorrhage. Blood pressure maintenance by infusion of volume expanders, such as crystalloids or colloids, may be insufficient to preserve the function of vital organs because they do not contain any oxygen-carrying molecules. If artificial RBCs were at hand, they could be used as a blood substitute until blood products are received from blood banks. This would save patients without degrading their quality of life. In the 1990s, we developed an artificial RBC in the form of a hemoglobin vesicle (Hb-V). Hb-V is a liposomal microparticle that encloses oxygen-carrying human Hb molecules. Different from RBCs, it has no blood type and is stable at room temperature, ensuring a long shelf-life. Its excellent biocompatibility and oxygen-carrying capacity have been proven in a number of animal experiments, and its production technique has also been established. Therefore, translational research is being designed with the aid of the Japan Agency of Medical Research and Development.


Assuntos
Substitutos Sanguíneos , Eritrócitos , Choque Hemorrágico/terapia , Pesquisa Médica Translacional , Animais , Hemoglobinas , Humanos , Japão , Oxigênio
10.
Phys Chem Chem Phys ; 21(41): 22902-22909, 2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31595895

RESUMO

Broken-symmetry density functional calculations have been used to calculate effective 55Mn hyperfine (A) tensors for a mixed-valence tetranuclear manganese complex, a model system for the S2 state of the oxygen-evolving complex of photosystem II. Recent investigations carried out in our laboratory showed that for calculations within the strong exchange limit, density functional calculations cannot reproduce the relative magnitude of the anisotropy of the hyperfine tensors of the MnIII center compared to the MnIV centers. In this work we therefore go beyond the strong exchange limit and investigate the effect of multiplet mixing, induced by zero-field splitting, on the effective hyperfine tensors through a perturbational treatment within the numerical spin projection procedure. Results show that the inclusion of zero-field splitting leads to a shift of the anisotropy from the MnIII ion towards the three MnIV ions, thus reconciling the calculated and experimentally observed anisotropy pattern. However, the final results are quite sensitive to the energy gap between the ground (doublet) and the first excited (quartet) state and therefore critically depend on the appropriate choice of the isotropic exchange coupling constants.


Assuntos
Anisotropia , Modelos Biológicos , Oxigênio/metabolismo , Complexo de Proteína do Fotossistema II , Manganês/química
11.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 31(9): 1118-1122, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31657336

RESUMO

OBJECTIVE: To systematically evaluate the clinical efficacies of Helmet non-invasive ventilation and oxygen therapy on patients with hypoxemic respiratory failure. METHODS: The randomized controlled trials (RCTs) for comparison of efficacy between Helmet non-invasive ventilation and oxygen therapy for treatment of patients with hypoxemic respiratory failure published by Wanfang database, China National Knowledge Infrastructure (CNKI), China Biology Medicine (CBM), PubMed, Embase, Cochrane Library and Web of Science were retrieved. The retrieval time was from the establishment of database to February 1st, 2019. The indexes of the study outcomes included oxygenation index, arterial partial pressure of carbon dioxide (PaCO2), endotracheal intubation rate, hospital mortality and intolerance rate. Literature search and data extraction was performed separately by two researchers. Quality assessment of literature was conducted according to the risk of bias criterion provided by Cochrane collaboration net. The extractive data were Meta-analyzed by RevMan 5.1.0. Funnel plot and Egger regression analysis was employed to detect publication bias. RESULTS: Six RCTs including 5 English studies and 1 Chinese study were selected. Finally, 547 patients were enrolled, with 270 patients in Helmet non-invasive ventilation group and 277 in oxygen therapy group. The study quality assessment revealed that the overall risk of bias was low, and no publication bias was detected by the funnel plot and Egger regression analysis. Meta-analysis showed that the oxygenation index in Helmet non-invasive ventilation group was significantly higher than that in oxygen therapy group [mean difference (MD) = 73.47, 95% confidence interval (95%CI) was 52.01 to 94.92, P = 0.000 01], and PaCO2 (MD = -2.46, 95%CI was -4.54 to -0.39, P = 0.02), endotracheal intubation rate [relative risk ratio (RR) = 0.38, 95%CI was 0.20 to 0.73, P = 0.004] and hospital mortality (RR = 0.35, 95%CI was 0.19 to 0.65, P = 0.000 8) in Helmet non-invasive ventilation group were significantly lower than those in oxygen therapy group. There was no significant difference in patient's intolerance between the two groups (RR = 2.38, 95%CI was 0.74 to 7.67, P = 0.15). CONCLUSIONS: Compared with oxygen therapy, the Helmet non-invasive ventilation used for treatment of patients with hypoxemic respiratory failure can effectively improve the oxygenation index, decrease the PaCO2, reduce the endotracheal intubation rate and hospital mortality, and the patients are well tolerated to the Helmet method.


Assuntos
Ventilação não Invasiva , Insuficiência Respiratória , China , Dispositivos de Proteção da Cabeça , Humanos , Oxigênio , Ensaios Clínicos Controlados Aleatórios como Assunto , Resultado do Tratamento
12.
Water Res ; 167: 115111, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31574347

RESUMO

The novel H2O2-MnO2 system was developed to achieve highly efficient membrane cleaning for both fouled PVDF and PES membranes in this study. Compared with conventional chemical cleaning process in which the whole membrane module had to be soaked in highly concentrated solution of chemical reagent for long period of time, the H2O2-MnO2 cleaning process conducting for only 5 min in 0.5 wt% H2O2 solution could achieve more than 95% recovery of permeate flux and almost total removal of the irreversible foulants. More importantly, the permeate flux and filtration efficiency of the membrane could be still kept stable after 6 runs of consecutive fouling and cleaning. Based on the systematic microscopic analyses, Electron Spin Resonance (ESR), Fourier Transform Infrared Spectroscopy (FTIR), as well as the quenching experiments with different free radical scavengers, the outstanding performance of H2O2-MnO2 system was attributed to the generation of both free radicals and abundant oxygen simultaneously, leading to the physico-chemical membrane cleaning. Conclusively, the newly developed H2O2-MnO2 system demonstrated noteworthy advantages on efficient membrane cleaning, and exhibited highly potential for the wide application in practical water treatment process.


Assuntos
Ultrafiltração , Purificação da Água , Peróxido de Hidrogênio , Compostos de Manganês , Membranas Artificiais , Óxidos , Oxigênio
13.
Medicine (Baltimore) ; 98(42): e17591, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31626131

RESUMO

BACKGROUND: Spinal cord ischemia-reperfusion injury (SCII) is a common complication of spinal surgery as well as thoracic and abdominal surgery. Acute cytotoxic edema is the key pathogenic alteration. Therefore, avoiding or decreasing cellular edema has become the major target for SCII treatment. METHODS: The antiedema activity of ginsenoside Rb1 on aquaporin (AQP) 4, nerve growth factor (NGF), and brain-derived neurotrophic factor expression was detected by western blot and real-time polymerase chain reaction under conditions of oxygen-glucose deprivation/reoxygenation (OGD/R) in a rat astrocyte model in vitro. In addition, the cellular membrane permeability of AQP4 overexpressing cells or AQP4 small interfering RNA-transfected cells was detected. RESULTS: Ginsenoside Rb1 significantly prevented OGD/R-induced AQP4 downregulation in rat astrocytes. In addition, ginsenoside Rb1 treatment or AQP4 overexpression in rat astrocytes significantly attenuated the OGD/R-induced increase of cellular membrane permeability. Moreover, ginsenoside Rb1 obviously prevented the OGD/R-induced decrease of NGF and BDNT expression in rat astrocytes. CONCLUSION: These findings demonstrate that ginsenoside Rb1 can relieve spinal cord edema and improve neurological function by increasing AQP4 expression.


Assuntos
Aquaporina 4/genética , Astrócitos/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Ginsenosídeos/farmacologia , Glucose/metabolismo , Oxigênio/metabolismo , Traumatismo por Reperfusão/genética , Animais , Animais Recém-Nascidos , Aquaporina 4/biossíntese , Astrócitos/efeitos dos fármacos , Astrócitos/patologia , Western Blotting , Células Cultivadas , Modelos Animais de Doenças , Ensaio de Imunoadsorção Enzimática , RNA/genética , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/tratamento farmacológico , Traumatismo por Reperfusão/patologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Medula Espinal/metabolismo , Medula Espinal/patologia
14.
Syst Appl Microbiol ; 42(6): 126016, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31635887

RESUMO

Hypoxic and anoxic niches of meromictic lakes are important sites for studying the microbial ecology of conditions resembling ancient Earth. The expansion and increasing global distribution of such environments also means that information about them serves to understand future phenomena. In this study, a long-term chemical dataset (1996-2015) was explored together with seasonal (in 2015) information on the diversity and abundance of bacterial and archaeal communities residing in the chemocline, monimolimnion and surface sediment of the marine meromictic Rogoznica Lake. The results of quantitative PCR assays, and high-throughput sequencing, targeting 16S rRNA genes and transcripts, revealed a clear vertical structure of the microbial community with Gammaproteobacteria (Halochromatium) and cyanobacteria (Synechococcus spp.) dominating the chemocline, Deltaproteobacteria and Bacteroidetes dominating the monimolimnion, and significantly more abundant archaeal populations in the surface sediment, most of which affiliated to Nanoarchaeota. Seasonal changes in the community structure and abundance were not pronounced. Diversity in Rogoznica Lake was found to be high, presumably as a consequence of stable environmental conditions accompanied by high dissolved carbon and nutrient concentrations. Long-term data indicated that Rogoznica Lake exhibited climate changes that could alter its physico-chemical features and, consequently, induce structural and physiological changes within its microbial community.


Assuntos
Biodiversidade , Lagos/microbiologia , Microbiota , Oxigênio/metabolismo , Archaea/classificação , Archaea/genética , Archaea/isolamento & purificação , Archaea/metabolismo , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Bactérias/metabolismo , Mudança Climática , Bases de Dados de Compostos Químicos , Irlanda , Lagos/química , Microbiota/genética , Oxigênio/análise , RNA Ribossômico 16S/genética , Estações do Ano , Microbiologia da Água
15.
Inorg Chem ; 58(20): 13933-13944, 2019 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-31566371

RESUMO

Density functional vibrational frequency calculations have been performed on eight geometry optimized cytochrome c oxidase (CcO) dinuclear center (DNC) reaction cycle intermediates and on the oxymyoglobin (oxyMb) active site. The calculated Fe-O and O-O stretching modes and their frequency shifts along the reaction cycle have been compared with the available resonance Raman (rR) measurements. The calculations support the proposal that in state A[Fea33+-O2-•···CuB+] of CcO, O2 binds with Fea32+ in a similar bent end-on geometry to that in oxyMb. The calculations show that the observed 20 cm-1 shift of the Fea3-O stretching mode from the PR to F state is caused by the protonation of the OH- ligand on CuB2+ (PR[Fea34+═O2-···HO--CuB2+] → F[Fea34+═O2-···H2O-CuB2+]), and that the H2O ligand is still on the CuB2+ site in the rR identified F[Fea34+═O2-···H2O-CuB2+] state. Further, the observed rR band at 356 cm-1 between states PR and F is likely an O-Fea3-porphyrin bending mode. The observed 450 cm-1 low Fea3-O frequency mode for the OH active oxidized state has been reproduced by our calculations on a nearly symmetrically bridged Fea33+-OH-CuB2+ structure with a relatively long Fea3-O distance near 2 Å. Based on Badger's rule, the calculated Fea3-O distances correlate well with the calculated νFe-O-2/3 (νFe-O is the Fea3-O stretching frequency) with correlation coefficient R = 0.973.


Assuntos
Teoria da Densidade Funcional , Complexo IV da Cadeia de Transporte de Elétrons/metabolismo , Ferro/química , Oxigênio/química , Biocatálise , Domínio Catalítico , Cristalografia por Raios X , Complexo IV da Cadeia de Transporte de Elétrons/química , Ferro/metabolismo , Ligantes , Modelos Moleculares , Oxigênio/metabolismo , Vibração
16.
Inorg Chem ; 58(20): 14244-14259, 2019 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-31595752

RESUMO

Near-IR-emitting and/or efficiently photodynamic water-soluble Ru(II) complexes that hold great application potentials as photodynamic therapy and/or photodetection agents for cancers have been poorly explored. In this paper, the solvatochromism, calf thymus DNA binding, and singlet oxygen generation properties of a known ruthenium(II) complex of visible-emitting [Ru(bpy)2(dtdpq)](ClO4)2 (Ru1) and a new homoleptic complex of near-IR-emitting [Ru(dtdpq)3](ClO4)2 (Ru2) (bpy = 2,2'-bipyridine, dtdpq = 2,3-bis(thiophen-2-yl)pyrazino[2,3-f][1,10]phenanothroline) in water are reported. Moreover, DNA photocleavage, singlet oxygen generation in HeLa cells, cellular uptake/localization, and in vitro photodynamic therapy for cancer cells of water-soluble Ru1 are described in detail. The results show that Ru1 acted as potent photodynamic cancer therapy and mitochondrial imaging agents. Ru2 exhibited very strong solvatochromism from a visible emission maximum at 588 nm in CH2Cl2 to the near-IR region at 700 nm in water and singlet oxygen generation yield in water (23%) and DNA binding properties (intercalative DNA binding constant on the order of 106 M-1) comparable to those of Ru1, which should make Ru2 attractive for the aforementioned applications of Ru1 if the water solubility of Ru2 can be improved enough for the studies above.


Assuntos
Antineoplásicos/farmacologia , Complexos de Coordenação/farmacologia , DNA de Neoplasias/efeitos dos fármacos , Luz , Fotoquimioterapia , Rutênio/farmacologia , Tiofenos/farmacologia , Células A549 , Antineoplásicos/síntese química , Antineoplásicos/química , Apoptose/efeitos dos fármacos , Sítios de Ligação/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Complexos de Coordenação/síntese química , Complexos de Coordenação/química , Células HeLa , Humanos , Raios Infravermelhos , Células MCF-7 , Estrutura Molecular , Oxigênio/análise , Oxigênio/metabolismo , Rutênio/química , Tiofenos/química
17.
J Strength Cond Res ; 33(11): 2913-2919, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31658211

RESUMO

Morris, KL, Widstrom, L, Goodrich, J, Poddar, S, Rueda, M, Holliday, M, San Millian, I, and Byrnes, WC. A retrospective analysis of collegiate athlete blood biomarkers at moderate altitude. J Strength Cond Res 33(11): 2913-2919, 2019-Blood biomarkers are used to assess overall health and determine positive/negative adaptations to training/environmental stimuli. This study aimed to describe the changes in blood biomarkers in collegiate football (FB) (n = 31) and cross-country (XC) (n = 29; 16 women [FXC], 13 men [MXC]) athletes across a competitive season while training and living at a moderate altitude (1,655 m). This study used a database of previously collected hematological (complete blood count and serum ferritin) and muscle damage (lactate dehydrogenase and creatine kinase) blood biomarkers. Data were analyzed both within and between groups using linear mixed-model and variance component analyses, alpha = 0.05. All 3 groups had significant but different patterns of change in the measured biomarkers. Hematological blood biomarkers increased at different time points but remained within the normal reference ranges with greater between-subject vs. within-subject variability, suggesting no significant decrements to oxygen-carrying capacity across the season for FB, MXC, or FXC. Muscle damage biomarkers increased over time and exceeded the normal reference ranges, indicating cell damage pathology. However, it is also possible that the demands of training and competition might alter baseline values in these athletes, although this cannot be confirmed with the current experimental design. The patterns of change in the hematological and muscle damage biomarkers varied by sport discipline, suggesting that the training/competitive environments of these athletes influence these changes. Further studies should assess how much training, altitude, and nutrition influence these changes by using a more comprehensive set of biomarkers and related performance parameters.


Assuntos
Aclimatação , Altitude , Biomarcadores/sangue , Esportes/fisiologia , Atletas , Creatina Quinase/sangue , Feminino , Ferritinas/sangue , Humanos , L-Lactato Desidrogenase/sangue , Masculino , Oxigênio , Estudos Retrospectivos , Universidades
18.
Bioresour Technol ; 294: 122150, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31569045

RESUMO

Simultaneous flue gas desulphurisation and denitrification in biotrickling filter was investigated under different O2 concentrations (0%, 3%, 5%, 8% and 10%) at 45 °C. NO and SO2 removal efficiency, intermediates (NO3-, NO2-, NO2, SO42- and S2-) interaction and accumulation, S0 recovery and microbial community structure were investigated. Results indicated the highest NO removal efficiency was 96.5% at 5% O2. Maximum SO2 removal efficiency was 95.6% at 3% O2. Moreover, N intermediates accumulation increased when O2 concentration increased from 0% to 10%. The lowest S2- concentration of 61 mg/L and the maximum S0 recovery of 76.9% were achieved at 5% O2. The bioreactor at 10% O2 contained less bacterial OTUs richness and evenness compared with other conditions. Illumina analysis indicated Proteobacteria, Firmicutes and Bacteroidetes were the dominant members. Overall, microbial community structure differs significantly under different O2 concentrations.


Assuntos
Microbiota , Dióxido de Enxofre , Reatores Biológicos , Óxido Nítrico , Oxigênio
19.
J Environ Manage ; 252: 109682, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31610444

RESUMO

When dual-chamber microbial fuel cell (MFC) is used to detect biochemical oxygen demand (BOD), dissolved oxygen is traditionally used as cathodic electron acceptor. The detection limit of this MFC-based BOD biosensor is usually lower than 200 mg/L. In this paper, the startup of MFC-based BOD biosensor was researched and the external resistor of MFC was optimized. Results showed that the MFC started up with the dissolved oxygen as cathodic electron acceptor within 10 d, and the external resistor was optimized as 500â€¯Ω to ensure the maximum output power of MFC. Dissolved oxygen and potassium permanganate (KMnO4) were used as cathodic electron acceptor to run MFC for detection of wastewater BOD, and the performances of two kinds of BOD biosensors were compared. The MFC-based BOD biosensor using KMnO4 (10 mmol/L) as cathodic electron acceptor exhibited an excellent performance, compared with that using dissolved oxygen. The upper limit of BOD detection was greatly broadened to 500 mg/L, the response time was shortened by 50% for artificial wastewater with a BOD of 100 mg/L, and the relative error of BOD detection was reduced to less than 10%. The MFC-based BOD biosensor using KMnO4 as cathodic electron acceptor showed a better linear relationship (R2 > 0.992) between the electric charge and BOD concentration within a BOD range of 25-500 mg/L. The MFC-based BOD biosensor using the KMnO4 as cathodic electron acceptor is promising with a better application prospect.


Assuntos
Fontes de Energia Bioelétrica , Técnicas Biossensoriais , Análise da Demanda Biológica de Oxigênio , Eletrodos , Elétrons , Oxigênio , Permanganato de Potássio
20.
Bioresour Technol ; 294: 122228, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31610496

RESUMO

Two aeration modes, namely point aeration and step aeration, were proposed and implemented in a full scale Orbal oxidation ditch, and nitrogen removal performance was studied. The results showed that nitrogen removal performance under point aeration mode depended on oxygen supply control. Highest total nitrogen (TN) removal efficiency of 73.2% was achieved when oxygen input in the outer, middle and inner channel accounted for 50, 25 and 25% of total oxygen supply, respectively. With the same oxygen supply, both aeration modes demonstrated complete nitrification with over 97% ammonia nitrogen removal efficiencies. However, TN removal efficiency was 78.8% under step aeration mode, which was higher than that under point aeration. The pyrosequencing results indicated that microbial community composition was affected by aeration modes and step aeration mode was beneficial to the enrichment of denitrifiers. The greater diversity and relative abundance of denitrifiers enhanced TN removal under step aeration mode.


Assuntos
Desnitrificação , Nitrogênio , Amônia , Reatores Biológicos , Nitrificação , Oxigênio
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