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1.
Water Sci Technol ; 80(3): 563-574, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31596267

RESUMO

This study was conducted to evaluate the influence of chloride ions (Cl-) on organic contaminants decolorization by the Fe0-activated persulfate process (PS/Fe0), as well as the generation of transformation products. Orange II (OII) was chosen as the target pollution. The results indicated that Cl- influenced the OII decolorization by PS/Fe0 system, resulting in the generation of chlorine-containing by-products. OII containing Cl- solution can be efficiently decolorized by PS/Fe0 process, and the decolorization efficiencies changed depending on Cl- concentration due to the reaction between Cl- and sulfate radicals (SO4-•). The operating cost for 94% color and 64% chemical oxygen demand (COD) removal of the OII dye was estimated at 0.73 USD/m3. The chlorine-containing by-products, such as chlorobenzene, 3,5-dichloro-benzene-1,2-diol, and 2,3-dichloro-2,3-dihydro-1,4-naphthoquinone, were generated during the reaction. The results further indicated that increasing both PS concentration and temperature enhanced OII decolorization and reduced the generation of chlorine-containing intermediates. The addition of ultrasound can further decrease the generation of chlorine-containing intermediates under high-temperature conditions. The proposed pathways of decolorization of OII containing Cl- also indicated that SO4-• dominated the OII degradation, while the presence of Cl- led to the generation of chlorine-containing intermediates.


Assuntos
Cloretos , Modelos Químicos , Sulfatos , Cor , Ferro/química , Oxirredução
2.
Adv Exp Med Biol ; 1161: 243-253, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31562634

RESUMO

Flavonoids are plant secondary metabolites that act as protectants against harmful effects of UV-B radiation inasmuch as biotic stress, conferring at the same time pigmentation of fruits and leaves [67]. The term "flavonoid" refers to phenolics having a basic skeleton of diphenylpropane (C6-C3-C6), which consists of two aromatic rings linked through three carbons that usually form an oxygenated heterocycle [25, 52]. Flavonoids are broken down into several different sub-categories based on their chemical structure. The main subclasses commonly found in food items are: flavonols, flavones, flavanones, flavan-3-ols, proanthocyanidins, and anthocyanins [44, 67]. Figure 19.1 depicts the major classification of flavonoids according to their chemical structure. Their occurrence in food matrices has been extensively reviewed [39, 44], and has been subject of extensive research in the last decades. Table 19.1 contains a few examples of compounds from each of the subcategory, with the fruit (berry) in which they are commonly found. The monomeric unit of flavonoids can dimerize and polymerize to form other important high molecular weight molecules; this is the case of proanthocyanidins, that are polymers of flavan-3-ols or flavanols. Not only do these compounds act as plant protectants, but they can also be very beneficial to human health. Cohorts studies performed in the early '90 have shown that dietary consumption of flavonoids was inversely associated with morbidity and mortality from coronary heart disease [31, 32]. These findings have opened an intensive field of research on the effects of flavonoids and flavonoids-rich food extracts in cardiovascular diseases (CVD) progression, particularly in the modulating CVD-associated oxidative stress and inflammation. In this short review, we will summarize the current findings in flavonoids beneficial effects in preventing CVD through inhibition of initial stages of CVD progression. Given the magnitude of scientific literature in the field, we will focus on two strictly mechanistic aspects: inhibition of chemical-induced LDL oxidation, and the effect of flavonoids in the monocyte/macrophages activation pathways.


Assuntos
Colesterol , Flavonoides , Metabolismo dos Lipídeos , Colesterol/metabolismo , Dieta , Flavonoides/metabolismo , Humanos , Inflamação/fisiopatologia , Oxirredução
3.
Chem Biol Interact ; 312: 108814, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31509734

RESUMO

Nanotechnology is a growing science that may provide several new applications for medicine, food preservation, diagnostic technologies, and sanitation. Despite its beneficial applications, there are several questions related to the safety of nanomaterials for human use. The development of nanotechnology is associated with some concerns because of the increased risk of carcinogenesis following exposure to nanomaterials. The increased levels of reactive oxygen species (ROS) that are due to exposure to nanoparticles (NPs) are primarily responsible for the genotoxicity of metal NPs. Not all, but most metal NPs are able to directly produce free radicals through the release of metal ions and through interactions with water molecules. Furthermore, the increased production of free radicals and the cell death caused by metal NPs can stimulate reduction/oxidation (redox) reactions, leading to the continuous endogenous production of ROS in a positive feedback loop. The overexpression of inflammatory mediators, such as NF-kB and STATs, the mitochondrial malfunction and the increased intracellular calcium levels mediate the chronic oxidative stress that occurs after exposure to metal NPs. In this paper, we review the genotoxicity of different types of metal NPs and the redox mechanisms that amplify the toxicity of these NPs.


Assuntos
Nanopartículas Metálicas/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Animais , Cálcio/metabolismo , Dano ao DNA/efeitos dos fármacos , Aditivos Alimentares/química , Humanos , Nanopartículas Metálicas/química , Nanopartículas Metálicas/uso terapêutico , Neoplasias/tratamento farmacológico , Oxirredução , Espécies Reativas de Oxigênio/metabolismo
4.
J Agric Food Chem ; 67(38): 10734-10743, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31479252

RESUMO

Glutenin is the main protein of flour and is a very important source of protein nutrition for humans. Methylglyoxal (MGO) is an important product of the Maillard reaction that occurs during the hot-processing of flour products, and it reacts with glutenin to facilitate changes in glutenin properties. Here, the effects of MGO on glutenin digestion during the heating process were investigated using a simulated MGO-glutenin system. MGO significantly reduced the digestibility of glutenin. The structure of MGO-glutenin and physicochemical properties were studied to understand the mechanism of the decrease of digestibility. These data suggest that changes in digestibility were caused by decreases in surface hydrophobicity and increases in disulfide bonds. MGO induces strong aggregation of glutenin after heating that led to the masking of cleavage sites for proteases. Moreover, carbonyl oxidation induced by MGO leads to intermolecular cross-linking of glutenin that increasingly masks or even destroys cleavage sites, further decreasing digestibility.


Assuntos
Glutens/química , Aldeído Pirúvico/química , Culinária , Digestão , Farinha/análise , Glutens/metabolismo , Temperatura Alta , Humanos , Interações Hidrofóbicas e Hidrofílicas , Reação de Maillard , Oxirredução , Aldeído Pirúvico/metabolismo
5.
J Agric Food Chem ; 67(38): 10563-10576, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31487171

RESUMO

Sulfur (S) metabolism plays a vital role in Cd detoxification, but the collaboration between melatonin biosynthesis and S metabolism under Cd stress remains unaddressed. Using exogenous melatonin, melatonin-deficient tomato plants with a silenced caffeic acid O-methyltransferase (COMT) gene, and COMT-overexpressing plants with cosuppression of sulfate transporter (SUT)1 and SUT2 genes, we found that melatonin deficiency decreased S accumulation and aggravated Cd phytotoxicity, whereas exogenous melatonin or overexpression of COMT increased S uptake and assimilation, resulting in an improved plant growth and Cd tolerance. Melatonin deficiency promoted Cd translocation from root to shoot, but COMT overexpression caused the opposite effect. COMT overexpression failed to compensate the functional hierarchy of S when its uptake was inhibited by cosilencing of transporter SUT1 and SUT2. Our study provides genetic evidence that melatonin-mediated tolerance to Cd is closely associated with the efficient regulation of S metabolism, redox homeostasis, and Cd translocation in tomato plants.


Assuntos
Cádmio/metabolismo , Lycopersicon esculentum/metabolismo , Melatonina/metabolismo , Enxofre/metabolismo , Transporte Biológico , Regulação da Expressão Gênica de Plantas , Lycopersicon esculentum/genética , Lycopersicon esculentum/crescimento & desenvolvimento , Proteínas de Membrana Transportadoras/genética , Proteínas de Membrana Transportadoras/metabolismo , Oxirredução , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Proteína O-Metiltransferase/genética , Proteína O-Metiltransferase/metabolismo
6.
Water Sci Technol ; 80(2): 365-376, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31537773

RESUMO

At different calcination conditions, titanium-based manganese oxides (MnOx) electrodes were fabricated by spraying method without adhesive. The MnOx/Ti electrodes were applied in electrochemical oxidation of wastewater treatment for the first time. The surface morphologies of electrodes were tested by scanning electron microscopy. The formation of different manganese oxidation states on electrodes was confirmed by X-ray diffraction and X-ray photoelectron spectroscopy. The electrochemical properties of the electrodes have been performed by means of cyclic voltammetry and electrochemical impedance spectroscopy. The characterizations revealed that the MnOx/Ti-350(20) electrode, prepared at calcination temperature of 350 °C for 20 min, exhibited fewer cracks on the electrode surface, larger electrochemically effective surface area and lower charge transfer resistance than electrodes prepared at other calcination conditions. Moreover, Acid Red B was used as target pollutant to test the electrode activity via monitoring the concentration changes by UV spectrophotometer. The results showed that the MnOx/Ti-350(20) electrode presented the best performance on decolorization of Acid Red B with the lowest cell potential during the process of electrochemical oxidation, and the chemical oxygen demand (COD) conversion was 50.7%. Furthermore, the changes of Acid Red B during the electrochemical oxidation process were proposed by the UV-vis spectra.


Assuntos
Compostos Azo/química , Técnicas Eletroquímicas , Eletrodos , Naftalenossulfonatos/química , Poluentes Químicos da Água/química , Oxirredução , Titânio
7.
Bioresour Technol ; 293: 122039, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31476562

RESUMO

An anaerobic ammonium oxidation-upflow anaerobic sludge blanket (anammox-UASB) reactor was operated without temperature control during the four seasons and was therefore subjected to natural periodic temperature variations between 9 and 28 ℃. The anammox reactor had a high nitrogen removal ability at intermediate and low temperatures. The total nitrogen (TN) concentration of the influent increased from 200 to 1200 mg/L, the nitrogen removal efficiency was maintained at 90%, and the nitrogen removal rate (NRR) increased to 9.15 ±â€¯0.35 kg N/m3/d. The enrichment of anammox bacteria in the UASB granular sludge reached 53.8%, and the dominant bacteria changed from Candidatus Brocadia to Candidatus Kuenenia after several seasons of cultivation. Dynamics analysis revealed that the maximum reaction rate of the anammox-UASB sludge was 62.5 kg N/m3/d, reflecting the high potential nitrogen removal ability of the reactor.


Assuntos
Compostos de Amônio , Esgotos , Anaerobiose , Reatores Biológicos , Nitrogênio , Oxirredução , Temperatura Ambiente
8.
Anticancer Res ; 39(9): 4795-4803, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31519581

RESUMO

BACKGROUND/AIM: To determine the mechanism of vitamin D3-induced modulation of antioxidant-related factors in endometrial cancer, we investigated their role in apoptosis of human endometrial cancer cells exposed to vitamin D3 Materials and Methods: The survival rate of human endometrial cancer cells was estimated after treatment with activated vitamin D3 Reactive oxygen species (ROS) levels were measured using flow cytometry. The levels of VDR, Trx, TXNIP and apoptosis-related proteins were investigated using western blotting and immunocytochemistry in human tissues. RESULTS: Treatment with D3 induced apoptotic cell death and cell-cycle arrest by increasing ROS concentration. Vitamin D3 inhibited proliferation of human endometrial cancer cells. It regulated intracellular ROS concentration in endometrial cancer cells via increased TXNIP expression. CONCLUSION: Antioxidant regulation via TXNIP is an important cell death mechanism in human endometrial cancer, and occurs via induction by vitamin D3.


Assuntos
Antioxidantes/metabolismo , Proteínas de Transporte/metabolismo , Neoplasias do Endométrio/metabolismo , Tiorredoxinas/metabolismo , Vitamina D/análogos & derivados , Apoptose/efeitos dos fármacos , Biomarcadores , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Neoplasias do Endométrio/tratamento farmacológico , Neoplasias do Endométrio/patologia , Feminino , Humanos , Imuno-Histoquímica , Oxirredução/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Vitamina D/farmacologia
9.
Waste Manag ; 97: 82-87, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31447030

RESUMO

Stable isotope measurements are an effective tool for evaluating methane (CH4) consumption in landfill soils. However, determining the extent of CH4 oxidation in soils using this approach can be inherently biased, depending on characteristics of the study site and the sampling strategy that is employed. In this study, we establish the unusual case that sampling at smaller scales captures a better representation of the degree of oxidation occurring in landfill cover soils. We did this by comparing three techniques (Plume, Probe, and Transect) that vary in the location of sampling within a site and in the areal footprint of each sample. The Plume method yielded estimates of CH4 oxidation that were 13-16% lower than the Transect and Probe methods, respectively. The Probe and Transect methods, two relatively small-scale and high resolution methods, the latter of which has not been previously described, are best suited to quantify CH4 oxidation in landfill soils as they demonstrably overcome the tendency of stable isotope methods to underestimate CH4 oxidation at the landfill scale. We recommend the use of these two sampling methods for monitoring the efficacy of landfill CH4 reduction strategies that are desired to help meet the goals of the Paris Agreement.


Assuntos
Gases de Efeito Estufa , Metano , Eliminação de Resíduos , Isótopos , Oxirredução , Paris , Solo , Instalações de Eliminação de Resíduos
10.
Chem Commun (Camb) ; 55(66): 9865-9868, 2019 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-31364624

RESUMO

Au hydrogel with a well-defined nanowire network was rationally designed through one-step dopamine-induced self-assembly. Due to the porous nanostructure along with the polydopamine induced accumulation and interfacial electron transfer effects, the resulting Au hydrogel nanozyme exhibits enhanced glucose oxidase-like activity and peroxidase-like activity, with a biomimetic cascade catalysis.


Assuntos
Biomimética , Dopamina/química , Glucose Oxidase/química , Ouro/química , Hidrogéis/química , Nanopartículas Metálicas/química , Peroxidase/química , Catálise , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Oxirredução
11.
Bioresour Technol ; 291: 121858, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31377515

RESUMO

The improved performances during anaerobic degradation of phenol to methane with Fe(OH)3 were usually inapparent, due to its lower solubility (unaccessible to dissimilatory iron reduction) and more positive reduction potential of Fe(III)/Fe(II) (unfavorable for enriching Fe(III)-reducing bacteria [IRBs]). In this study, citrate, the organic chelates, were used to solubilize Fe(III) with the aim of improving the phenol degradation and declining the reduction potential of Fe(III)/Fe(II). Results showed that, in the co-occurrence of citrate and Fe(OH)3, the degradation rates of phenol were about 1.3-fold rapider than that with sole Fe(OH)3. Analysis of cyclic voltammetry demonstrated that the reduction potential of Fe(III)/Fe(II) in the form of Fe(OH)3 (-0.41 to -0.28 V vs Ag/AgCl) declined to -0.61 to -0.41 V. As a result, the Fe(III)-reducing genera, such as Petrimonas and Shewanella, which held a great potential of proceeding syntrophic metabolism via direct interspecies electron transfer (DIET), were significantly enriched.


Assuntos
Quelantes/farmacologia , Compostos Férricos/metabolismo , Ferro/metabolismo , Metano/metabolismo , Fenol/metabolismo , Anaerobiose , Bacteroides/efeitos dos fármacos , Bacteroides/metabolismo , Transporte de Elétrons , Oxirredução , Fenóis/metabolismo , Shewanella/efeitos dos fármacos , Shewanella/metabolismo
12.
Top Curr Chem (Cham) ; 377(5): 22, 2019 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-31444578

RESUMO

A methodology for photocatalytic reactor modeling applied to advanced oxidation processes for chemical pollution abatement is presented herein. Three distinct reactor configurations typically employed in the field of air and water purification-wall reactors, slurry reactors, and fixed-bed reactors-are considered to illustrate the suggested approach. Initially, different mechanistically derived kinetic expressions to represent the photocatalytic rate of pollutant degradation are reviewed, indicating the main assumptions made by the authors in the published contributions. These kinetic expressions are needed to solve the mass balances of the reactant species in the photocatalytic reactors. As is well known, at least one of the steps of the reaction mechanism requires evaluation of the rate of electron-hole generation, which depends on the photon absorption rate: a volumetric property for reactions with the catalyst particles in aqueous suspension or a surface property for systems with a fixed catalyst deposited on an inert support. Subsequently, the different techniques for evaluating the optical properties of slurry and immobilized systems, and the numerical methods applied to calculate the photon absorption rate, are described. The experimental and theoretical results of pollutant degradation in each reactor type are then presented and analyzed. Finally, the definition, calculation, and relevance of different efficiency parameters are briefly reviewed. Using these illustrative examples, we emphasize the need for a systematic and rigorous approach for photocatalytic reactor modeling in order to overcome the inherent drawbacks of photocatalysis and to improve the overall efficiency of the process.


Assuntos
Luz , Modelos Teóricos , Poluentes Químicos da Água/química , Catálise , Ácido Clofíbrico/química , Cinética , Oxirredução , Fótons , Titânio/química
13.
Phys Chem Chem Phys ; 21(32): 17971-17977, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31384846

RESUMO

The electron-hole injection from a family of spiropyran photoswitches into A/T-duplex DNA has been investigated at the molecular level for the first time. Multiscale computations coupled with automatized quantitative wavefunction analysis reveal a pronounced directionality and regioselectivity towards the template strand of the duplex DNA. Our findings suggest that this directional and regioselective photoinduced electron-hole transfer could thus be exploited to tailor the charge transport processes in DNA in specific applications.


Assuntos
Benzopiranos/química , DNA/química , Indóis/química , Substâncias Intercalantes/química , Nitrocompostos/química , Transporte de Elétrons , Luz , Modelos Moleculares , Conformação de Ácido Nucleico , Oxirredução , Processos Fotoquímicos , Prótons , Termodinâmica
14.
J Forensic Leg Med ; 67: 37-48, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31419763

RESUMO

Previous studies have begun to characterize the microbial community dynamics of the skin, soil, gut, and oral cavities of decomposing remains. One area that has yet to be explored in great detail is the microbiome of the fly larval mass, the community of immature flies that plays a significant role in decomposition. The current study aimed to characterize the microbiology and chemistry of larval masses established on pig (Sus scrofa domesticus) carcasses and to determine if these characteristics have potential as temporal evidence. Carcasses (n = 3) were decomposed on the soil surface of a tropical habitat on Oahu, Hawaii, USA and sampled over three days at 74 h, 80 h, 98 h, 104 h, 122 h, and 128 h (∼85-142 Accumulated Degree Days) postmortem. Larval masses were analyzed via high-throughput 16S rRNA sequencing and in situ chemical measurements (pH, temperature, oxidation-reduction potential). A trend was observed that resulted in three distinct microbial communities (pre-98 h, 98 h, and post-98 h). The oxidation-reduction potential (Eh) of larval masses apparently regulated microbial community structure with the most negative Eh being associated with the least rich and diverse microbial communities. Overall, a significant interaction between time and taxa was observed, particularly with bacterial phyla Firmicutes and Proteobacteria. The current results provide new insight into the microbial community and chemical parameters of larval masses and indicate a temporal shift that could be further studied as a PMI estimator.


Assuntos
Comportamento Alimentar , Larva/química , Larva/microbiologia , Microbiota , Mudanças Depois da Morte , Animais , Bactérias/genética , Entomologia , Patologia Legal , Hawaii , Concentração de Íons de Hidrogênio , Modelos Animais , Oxirredução , RNA Ribossômico 16S , Análise de Sequência de RNA , Suínos
15.
J Microbiol ; 57(9): 738-747, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31376106

RESUMO

The simultaneous removal of phenol and selenite from synthetic wastewater was investigated by adopting two different co-culturing techniques using the fungus Phanerochaete chrysosporium and the bacterium Delftia lacustris. Separately grown biomass of the fungus and the bacterium (suspended co-culture) was incubated with different concentrations of phenol (0-1,200 mg/L) and selenite (10 mg/L). The selenite ions were biologically reduced to extracellular Se(0) nanoparticles (3.58 nm diameter) with the simultaneous degradation of up to 800 mg/L of phenol. Upon growing the fungus and the bacterium together using an attached growth co-culture, the bacterium grew as a biofilm onto the fungus. The extracellularly produced Se(0) in the attached growth co-culture had a minimum diameter of 58.5 nm. This co-culture was able to degrade completely 50 mg/L phenol, but was completely inhibited at a phenol concentration of 200 mg/L.


Assuntos
Delftia/metabolismo , Phanerochaete/metabolismo , Fenol/metabolismo , Ácido Selenioso/metabolismo , Selênio/metabolismo , Biodegradação Ambiental , Biotransformação , Técnicas de Cocultura , Delftia/crescimento & desenvolvimento , Oxirredução , Phanerochaete/crescimento & desenvolvimento , Águas Residuárias/microbiologia , Poluentes da Água/metabolismo
16.
Bioresour Technol ; 291: 121885, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31377049

RESUMO

Lignin is the most abundant, renewable aromatic resource on earth and holds great potential for the production of value-added chemicals. The efficient valorization of lignin requires to deal with several formidable challenges, especially to prevent it from re-condensation reactions during its depolymerization. Recently, a strategy involving the activation of lignin side chains by selective oxidation of the benzylic alcohol in ß-O-4 linkages to facilitate lignin degradation to aromatic monomers has become very popular. This strategy provides great advantages for lignin selective degradation to high yields of aromatics under mild conditions, but requires an additional pre-oxidation step. The purpose of this review is to provide the latest cutting-edge innovations of this novel approach. Various catalytic systems, including those using chemo-catalytic methods, physio-chemo catalytic methods, and/or bio-catalytic methods, for the oxidative activation of lignin side chains are summarized. By analyzing the current situation of lignin depolymerization, certain promising directions are emphasized.


Assuntos
Lignina/química , Catálise , Oxirredução , Polimerização
17.
Science ; 365(6456): 866-867, 2019 08 30.
Artigo em Inglês | MEDLINE | ID: mdl-31467210
18.
Chem Commun (Camb) ; 55(68): 10142-10145, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31389424

RESUMO

Hydrogen sulfide, an endogenous signalling molecule, is central to several pathophysiological processes in mammalian systems. It scavenges reactive oxygen species and is known to ameliorate dopaminergic neuronal degeneration in neurotoxin-induced Parkinson's disease models. The rapid volatilization of H2S from spontaneously releasing sulfide salts being a challenge, we describe peptide conjugates which exhibit tris(2-carboxyethyl)phosphine mediated "slow and sustained" H2S release. These conjugates reduced hydrogen peroxide-induced oxidative stress and significantly increased dopamine levels in transgenic C. elegans.


Assuntos
Dopamina/metabolismo , Sulfeto de Hidrogênio/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Peptídeos/farmacologia , Tionas/farmacologia , Tiofenos/farmacologia , Animais , Animais Geneticamente Modificados , Anti-Inflamatórios/metabolismo , Antioxidantes/metabolismo , Caenorhabditis elegans/genética , Liberação Controlada de Fármacos , Oxirredução , Peptídeos/síntese química , Peptídeos/química , Fosfinas/química , Pró-Fármacos/síntese química , Pró-Fármacos/química , Pró-Fármacos/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Tionas/síntese química , Tionas/química , Tiofenos/síntese química , Tiofenos/química , alfa-Sinucleína/genética
19.
An Acad Bras Cienc ; 91(3): e20180646, 2019 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-31411259

RESUMO

The hepatoprotective effects of the ethanolic extracts of propolis (EEP) on alcohol-induced liver steatosis were investigated in Wistar rats. Chronic alcoholic fatty liver was induced by administration of 52% alcohol to male Wistar rats at the dose of 1% body weight for 7 weeks. Then animals were simultaneously treated with 50% ethanol solutions of EEP or normal saline at the dose of 0.1% body weight for 4 further weeks. Serological analyses and liver histopathology studies were performed to investigate the development of steatosis. Microarray analysis was conducted to investigate the alterations of hepatic gene expression profiling. Our results showed that 4-week treatment of EEP helped to restore the levels of various blood indices, liver function enzymes and the histopathology of liver tissue to normal levels. Results from the microarray analysis revealed that the hepatic expressions of genes involved in lipogenesis were significantly down-regulated by EEP treatment, while the transcriptional expressions of functional genes participating in fatty acids oxidation were markedly increased. The ability of EEP to reduce the negative effects of alcohol on liver makes propolis a potential natural product for the alternative treatment of alcoholic fatty liver.


Assuntos
Fígado Gorduroso Alcoólico/metabolismo , Hepatopatias Alcoólicas/metabolismo , Extratos Vegetais/metabolismo , Própole/metabolismo , Substâncias Protetoras/metabolismo , Alanina Transaminase/metabolismo , Animais , Apiterapia/métodos , Aspartato Aminotransferases/metabolismo , Colesterol/metabolismo , Modelos Animais de Doenças , Etanol , Ácidos Graxos/biossíntese , Fígado Gorduroso Alcoólico/tratamento farmacológico , Fígado Gorduroso Alcoólico/genética , Fígado Gorduroso Alcoólico/patologia , Hepatopatias Alcoólicas/tratamento farmacológico , Hepatopatias Alcoólicas/genética , Hepatopatias Alcoólicas/patologia , Masculino , Oxirredução , Extratos Vegetais/química , Extratos Vegetais/uso terapêutico , Própole/química , Própole/uso terapêutico , Substâncias Protetoras/química , Substâncias Protetoras/uso terapêutico , Ratos Wistar , Análise Serial de Tecidos/métodos , Transcrição Genética/genética , Triglicerídeos/metabolismo
20.
Dokl Biochem Biophys ; 486(1): 197-200, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31367820

RESUMO

The oxidative modification of human hemoglobin (Hb) treated with hydrogen peroxide was investigated. Using the mass spectrometry method, the oxidized amino acid residues of the hemoglobin molecule were detected: αTrp14, αTyr24, αArg31, αMet32, αTyr42, αHis45, αHis72, αMet76, αPro77, αLys90, αCys104, αTyr140, ßHis2, ßTrp15, ßTrp37, ßMet55, ßCys93, ßCys112, ßTyr130, ßLys144, and ßHis146. The antioxidant potential of the Hb molecule in the intracellular space and in the blood plasma is discussed.


Assuntos
Hemoglobinas/metabolismo , Peróxido de Hidrogênio/farmacologia , Humanos , Oxirredução/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos
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