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1.
Scand J Immunol ; 90(3): e12799, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31211854

RESUMO

Pemphigus vulgaris (PV) is an autoimmune disease characterized by the production of IgG autoantibodies owing to an imbalance in the Th1/Th2 and Th17/Tregs cell pathways. The role of gut microbiota in the development of immune system and autoimmune diseases has been unraveled in the last two decades. However, data pertaining to gut microbiota of PV patients is largely lacking. We aimed to compare the gut microbiota of PV patients and healthy controls and assessed potential correlation with circulating cytokines of Th1/Th2/Th17 cell. Faecal bacterial diversity was analysed in 18 PV patients and 14 age- and gender-matched healthy individuals using hypervariable tag sequencing of the V3-V4 region of the 16S rRNA gene. Plasma levels of 20 inflammatory cytokines were assessed using the Luminex screening system. As a result, we identified 10 differentially abundant taxa between patients and controls. At the genera level, Lachnospiracea_incertae_sedis and Coprococcus decreased, while Granulicatella, Flavonifractor enriched in PV. Plasma levels of C5a, interleukin (IL)-2R, IL-6, IL-8, IL-7, IL-1ß, IL17A, IL-5 and IL-21 were significantly increased in PV Flavonifractor exhibited a positive correlation with C5a, IL-6, IL-8, IL-7, IL-1ß, IL17A and IL-21. Lachnospiracea_incertae_sedis and Coprococcus showed a negative correlation with IL-17A. Our results are consistent with the hypothesis that PV patients have gut microbial dysbiosis which might contribute to the immune disorder and the development of PV.


Assuntos
Doenças Autoimunes/imunologia , Citocinas/imunologia , Microbioma Gastrointestinal/imunologia , Inflamação/imunologia , Pênfigo/imunologia , Plasma/imunologia , Adulto , Autoanticorpos/imunologia , Doenças Autoimunes/microbiologia , Fezes/microbiologia , Feminino , Humanos , Inflamação/microbiologia , Masculino , Pessoa de Meia-Idade , Pênfigo/microbiologia , Plasma/microbiologia , Linfócitos T Reguladores/imunologia , Linfócitos T Reguladores/microbiologia , Células Th1/imunologia , Células Th1/microbiologia , Células Th17/imunologia , Células Th17/microbiologia , Células Th2/imunologia , Células Th2/microbiologia
2.
J Oral Pathol Med ; 47(3): 281-285, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29292532

RESUMO

OBJECTIVES: Oral dysesthesia (burning mouth syndrome) is characterized by a burning-like sensation of the oral mucosa. The etiology of this disorder is still unknown, however, associations with oral fungal carriage have been proposed and applied clinically. The aim of the this study was to compare oral Candida carriage in patients with oral dysesthesia with Candida carriage in patients with other commonly diagnosed oral diseases to clarify the relationship between Candida and oral dysesthesia. SUBJECTS AND METHODS: In total, 441 patients in total including 79 patients diagnosed with oral dysesthesia were included in this study. A retrospective analysis of mycological investigations undertaken in patients with clinically diagnosed oral dysesthesia compared with other oral conditions was undertaken. RESULTS: Oral carriage of Candida was found in 63.3% (50 of 79) of patients with oral dysesthesia. The frequency of carriage and oral load of Candida were not significantly increased in patients with oral dysesthesia relative to the other conditions assessed. Patients with clinical signs of fungal infection or xerostomia presented with increased carriage of Candida. CONCLUSION: There is no association between oral dysesthesia and the presence or load of oral Candida.


Assuntos
Síndrome da Ardência Bucal/microbiologia , Candida/isolamento & purificação , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma de Células Escamosas/microbiologia , Contagem de Colônia Microbiana , Feminino , Humanos , Hiperplasia , Líquen Plano Bucal/microbiologia , Masculino , Pessoa de Meia-Idade , Mucosa Bucal/microbiologia , Mucosa Bucal/patologia , Neoplasias Bucais/microbiologia , Pênfigo/microbiologia , Estudos Retrospectivos , Xerostomia/microbiologia , Adulto Jovem
3.
Int J Med Microbiol ; 307(6): 291-296, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28579477

RESUMO

Exfoliative toxin B (ETB) encoded by some large plasmids plays a crucial role in epidermolytic diseases caused by Staphylococcus aureus. We have found as yet unknown types of etb gene-positive plasmids isolated from a set of impetigo strains implicated in outbreaks of pemphigus neonatorum in Czech maternity hospitals. Plasmids from the strains of clonal complex CC121 were related to archetypal plasmid pETBTY4. Sharing a 33-kb core sequence including virulence genes for ETB, EDIN C, and lantibiotics, they were assigned to a stand-alone lineage, named pETBTY4-based plasmids. Differing from each other in the content of variable DNA regions, they formed four sequence types. In addition to them, a novel unique plasmid pETB608 isolated from a strain of ST130 was described. Carrying conjugative cluster genes, as well as new variants of etb and edinA genes, pETB608 could be regarded as a source of a new lineage of ETB plasmids. We have designed a helpful detection assay, which facilitates the precise identification of the all described types of ETB plasmids.


Assuntos
Dermotoxinas/genética , Exfoliatinas/genética , Impetigo/microbiologia , Plasmídeos/genética , Staphylococcus aureus/genética , Proteínas de Bactérias/genética , Bacteriocinas/genética , República Tcheca/epidemiologia , DNA Bacteriano/genética , Humanos , Impetigo/epidemiologia , Pênfigo/epidemiologia , Pênfigo/microbiologia , Filogenia , Plasmídeos/isolamento & purificação , Staphylococcus aureus/classificação , Virulência/genética , Sequenciamento Completo do Genoma
4.
Dermatol Online J ; 21(11)2015 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-26632939

RESUMO

Pemphigus vulgaris is an immunobullous disease characterized by intraepidermal blister formation. These blisters eventually rupture, leaving erosions that are slow to heal, often leaving hyperpigmented patches, but no scars. We describe a case of a 67- year-old man with pemphigus vulgaris who suffered severe keloidal scarring after the pemphigus lesions became infected. His keloids were treated with intralesional corticosteroids with some improvement. Pemphigus vulgaris, a process confined to the epidermis, may lead to scarring in predisposed individuals, particularly if infection occurs.


Assuntos
Queloide/etiologia , Pênfigo/complicações , Infecções Cutâneas Estafilocócicas/complicações , Corticosteroides/uso terapêutico , Idoso , Fármacos Dermatológicos/uso terapêutico , Quimioterapia Combinada , Humanos , Queloide/tratamento farmacológico , Masculino , Pênfigo/tratamento farmacológico , Pênfigo/microbiologia , Prednisona/uso terapêutico , Rituximab/uso terapêutico , Infecções Cutâneas Estafilocócicas/tratamento farmacológico , Staphylococcus aureus , Triancinolona/uso terapêutico
7.
Res Microbiol ; 161(4): 260-7, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20382218

RESUMO

We have isolated and characterized two distinct types of exfoliative toxin A (ETA)-converting bacteriophages originating from Staphylococcus aureus strains responsible for massive outbreaks of pemphigus neonatorum in the Czech Republic. Three induced phages designated as ph iB531, phi B557 and phi B122 were found to be capable of transferring the eta gene into the prophageless non-toxigenic S. aureus strain and converting it into an ETA producer. Comparisons of the phage sequences derived from 12 selected genes and 2 genomic segments (polymorphic P2 and conserved C4) revealed that phi B531 and phi B557 were identical each other, but phi B122 differed from them in 5 gene sequences, the xis gene content and the virion protein profile. Thus, phi B122 represents a new type of still undescribed ETA-converting phage. This study highlights not only the conclusive genomic diversity of eta gene-positive phages, but also their virulence implications in impetigo S. aureus strains.


Assuntos
Exfoliatinas/genética , Variação Genética , Pênfigo/microbiologia , Infecções Estafilocócicas/microbiologia , Fagos de Staphylococcus/genética , Fagos de Staphylococcus/isolamento & purificação , Staphylococcus aureus/virologia , República Tcheca/epidemiologia , Surtos de Doenças , Exfoliatinas/metabolismo , Transferência Genética Horizontal , Humanos , Dados de Sequência Molecular , Pênfigo/epidemiologia , Infecções Estafilocócicas/epidemiologia , Fagos de Staphylococcus/metabolismo , Staphylococcus aureus/genética , Staphylococcus aureus/metabolismo
8.
Diagn Microbiol Infect Dis ; 66(3): 248-52, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19903585

RESUMO

The exfoliative toxin A (ETA) is encoded by the gene located on Staphylococcus aureus prophages. We have developed a single-reaction multiplex polymerase chain reaction (PCR) assay for rapid and specific detection of various phiETA prophages of serogroup B responsible for dissemination of eta gene and ETA production in clinical strains. This PCR strategy enabled to classify the ETA-positive strains into 6 groups designated ETA-B1, ETA-B2, ETA-B3, ETA-B4, ETA-B5, and ETA-B6. The method was tested on a diverse set of 101 ETA and/or ETB-positive S. aureus strains isolated in 22 Czech maternity hospitals and 1 Slovak maternity hospital between 1998 and 2009. This novel PCR strategy is reliable in the rapid identification of yet undescribed ETA-converting B prophages and differentiation of the closely related ETA-positive strains, and it is a convenient tool for hospital epidermolytic infection control.


Assuntos
Exfoliatinas/genética , Reação em Cadeia da Polimerase/métodos , Prófagos/genética , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/classificação , Técnicas de Tipagem Bacteriana/métodos , Tchecoslováquia , Feminino , Maternidades , Humanos , Recém-Nascido , Pênfigo/microbiologia , Gravidez , Pele/microbiologia , Staphylococcus aureus/genética
9.
Br J Dermatol ; 148(3): 526-32, 2003 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-12653745

RESUMO

BACKGROUND: Glycocalyx collapses during dehydration to produce electron-dense accretions. Confocal laser scanning microscopy (CLSM) may be used to visualize fully hydrated microbial biofilms. OBJECTIVES: Using CLSM, to analyse glycocalyx production by Staphylococcus aureus cells in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus. A second objective was to compare numbers of S. aureus cells in tissue sections prepared by different methods for routine light microscopy. METHODS: S. aureus cells in skin lesions of impetigo, atopic dermatitis and pemphigus were stained with safranin, and positive staining with fluorescein isothiocyanate-conjugated concanavalin A was considered to indicate the presence of glycocalyx. RESULTS: All S. aureus cells tested in skin lesions of impetigo, atopic dermatitis and pemphigus were covered with glycocalyx and formed microcolonies. The numbers of S. aureus cells in a routine light microscopy section were significantly lower than those in a frozen section that had not been dehydrated with ethanol. CONCLUSIONS: S. aureus cells generally produce glycocalyx in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus, which accounts for the difficulty of removing S. aureus cells from these skin lesions. The glycocalyx may collapse during dehydration and most of the S. aureus cells may be carried away during preparation of routine light microscope sections.


Assuntos
Biofilmes/crescimento & desenvolvimento , Dermatite Atópica/microbiologia , Glicocálix/metabolismo , Impetigo/microbiologia , Microscopia Confocal/métodos , Pênfigo/microbiologia , Staphylococcus aureus/fisiologia , Ácido Acético/farmacologia , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Contagem de Colônia Microbiana , Feminino , Fibrina , Ácido Fusídico/farmacologia , Humanos , Ácido Clorídrico/farmacologia , Masculino , Pessoa de Meia-Idade , Staphylococcus aureus/efeitos dos fármacos
10.
Acta Chir Plast ; 45(3): 83-7, 2003.
Artigo em Inglês | MEDLINE | ID: mdl-14733251

RESUMO

The case report is describing patient with an autoimmune disease, Pemphigus Vulgaris. This patient arrived to the general practitioner with Pemphigus Vulgaris after 20 days from the first manifestation of the disease. Despite intensive care, patient dies forty-seventh day after first clinical manifestation of the illness of candidemia. Candidiasis was diagnosed only in postmortem examination.


Assuntos
Candidíase/etiologia , Fungemia/etiologia , Pênfigo/diagnóstico , Pênfigo/microbiologia , Candidíase/patologia , Evolução Fatal , Feminino , Fungemia/patologia , Humanos , Pênfigo/tratamento farmacológico , Pênfigo/patologia
11.
Appl Environ Microbiol ; 68(5): 2572-5, 2002 May.
Artigo em Inglês | MEDLINE | ID: mdl-11976137

RESUMO

Buchnera strains from most aphid subfamilies studied to date have been found to carry the leucine gene cluster (leuA, -B, -C, and -D) on a plasmid, an organization unique among bacteria. Here, however, we demonstrate a classical chromosomal location of the cluster in Buchnera sp. strain PSY from the aphid Pemphigus spyrothecae (subfamily Pemphiginae). The genes that flank leuABCD in Buchnera sp. strain PSY appear to be adjacent in the genome of Buchnera sp. strain APS, a strain carrying a leucine plasmid. We propose that the presence of a leucine plasmid predates the diversification of symbiotic Buchnera and that the chromosomal location observed in Buchnera sp. strain PSY arose by a transfer of the leucine genes from a plasmid to the chromosome.


Assuntos
Buchnera/metabolismo , Hidroliases , Leucina/metabolismo , Família Multigênica , Pênfigo/microbiologia , Simbiose/fisiologia , Animais , Proteínas de Bactérias/genética , Sequência de Bases , Buchnera/genética , Clonagem Molecular , DNA Bacteriano/análise , Amplificação de Genes , Leucina/genética , Dados de Sequência Molecular
13.
Acta Otolaryngol Suppl ; 524: 73-8, 1996.
Artigo em Inglês | MEDLINE | ID: mdl-8790766

RESUMO

We report on a case of pemphigus vulgaris with lesions in the oral cavity as well as an outline of differential diagnosis and treatment. Changes in the pemphigus antibodies in blood assisted in judging the therapeutic effects along with the therapeutic course.


Assuntos
Pênfigo/diagnóstico , Adulto , Canal Anal/microbiologia , Candida/isolamento & purificação , Diagnóstico Diferencial , Humanos , Masculino , Pênfigo/microbiologia , Pênis/microbiologia , Estomatite/diagnóstico
14.
Mycoses ; 37(3-4): 137-9, 1994 Mar-Apr.
Artigo em Inglês | MEDLINE | ID: mdl-7845420

RESUMO

We report the case of a 48-year old man who after a disease-free interval of 7 years developed a relapse of pemphigus vulgaris (PV) presenting as pompholyx of the left foot. The patient was first thought to suffer from a dyshidrosic form of tinea pedis due to Trichophyton rubrum. Although the concomitant occurrence of a dermatomycosis and PV has been described previously, our case illustrates an unusual and misleading presentation of PV, which may delay the correct diagnosis.


Assuntos
Pênfigo/complicações , Tinha/complicações , Trichophyton/isolamento & purificação , Humanos , Masculino , Pessoa de Meia-Idade , Pênfigo/tratamento farmacológico , Pênfigo/microbiologia , Pênfigo/patologia , Tinha/tratamento farmacológico , Tinha/microbiologia , Tinha/patologia
15.
J Infect ; 20(1): 73-82, 1990 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-2299186

RESUMO

During the summer of 1987, an epidemic of pemphigus neonatorum took place at Guy's Hospital. It involved more than 80 neonates in the maternity unit. Swabs from the umbilical stumps of the babies and from the noses of several attending midwives yielded Staphylococcus aureus of phage-type Group II 3A/3C. Despite an extensive disinfection policy, which included identification and treatment of carriers, the outbreak persisted for 3 months. Final resolution came only after detailed epidemiological research revealed those midwives most likely to be involved. After these had been singled out for further treatment, the outbreak ended. The epidemic strains were later subjected to reverse phage-typing, plasmid profiling and in vivo testing for production of epidermolytic toxin in order to confirm true carriers and cases. Retrospective analysis identified those persons most likely to have been responsible for propagation of the epidemic strain. The exact course of the outbreak was then clarified.


Assuntos
Infecção Hospitalar/epidemiologia , Surtos de Doenças , Pênfigo/epidemiologia , Infecção Hospitalar/tratamento farmacológico , Infecção Hospitalar/microbiologia , Floxacilina/uso terapêutico , Humanos , Recém-Nascido , Nariz/microbiologia , Pênfigo/tratamento farmacológico , Pênfigo/microbiologia , Estudos Retrospectivos , Staphylococcus aureus/isolamento & purificação , Umbigo/microbiologia
16.
Ann Dermatol Venereol ; 104(12): 811-5, 1977 Dec.
Artigo em Francês | MEDLINE | ID: mdl-207214

RESUMO

The fragility of epidermal cells in Hailey-Hailey's disease end even more in Darier's disease seems to favour the development of viral infections. The authors present six cases of herpes virus infections in three siblings with an intermediate form between Darier's disease and Hailey-Hailey's disease, both having a tendency to recur. One of the flares demonstrated signs of Kaposi and Juliusberg's pustulosis vacciniformis which is often found in the literature as a complication of Darier's disease. The authors discuss the frequency of the recurrences, which could be due to different viruses. Herpetic virus is often found, less frequently vaccinial virus, but sometimes also Coxsackie A 16 virus.


Assuntos
Doença de Darier/complicações , Pênfigo/complicações , Viroses/epidemiologia , Adolescente , Adulto , Criança , Doença de Darier/microbiologia , Diagnóstico Diferencial , Feminino , França , Infecções por Herpesviridae/epidemiologia , Infecções por Herpesviridae/microbiologia , Humanos , Masculino , Pênfigo/microbiologia , Viroses/microbiologia , Vírus/isolamento & purificação
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