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1.
Microb Pathog ; 132: 117-123, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31009656

RESUMO

In this study, we isolated four Weissella confusa strains from the healthy horse feces to test their potential as equine probiotics. The identification and characteristics of these isolates were determined as per standard methods. Resistance and susceptibility of the isolated strains were tested to low pHs, different heat treatments, commonly used antibiotics and against the pathogenic strains of Salmonella, Pasteurella, Staphylococcus aureus, and Escherichia coli. After 3 h cultural in different pH medium, the 4 strains still had a certain amount of survival above pH 3.0. WH2 and WH4 were still viable at pH2.5. All the isolated strains showed proper growth at 60 °C while no strain survived at 80 °C. The inhibition of α-amylase, the scavenging ability of free radical DPPH· and hydroxyl free radical HO·were also investigated. The results showed that WH4 had highest inhibition rate of α-amylase activity and DPPH· free radical scavenging rate, and the inhibition rate of α-amylase activity was 24.09% and the DPPH· free radical scavenging rate was 35.78%. The inhibition rate ofα-amylase activity and DPPH· scavenging rate of free radicals in the other three strains were about 10%. The clearance rate of hydroxyl radical (HO·) in 4 strains was between 12% and 15%. The antibiotic susceptibilities varied for these four Weisella strains but all of them showed resistance against the frequently used equine antibiotics. All the four strains successfully suppressed the growth of standard strains in in vitro bacteriostasis experiment, which included Salmonella enteritidis (NTNC13349), Escherichia coli (C83902) and Staphylococcus aureus (BNCC186335). they also successfully suppressed the growth of state key laboratory isolating pathogens, which are Pasterurella multocida and Salmonella. Our findings suggest that the isolated strains of Weissella confusa can act as potential equine probiotics and should be explored further.


Assuntos
Fezes/microbiologia , Probióticos/isolamento & purificação , Weissella/isolamento & purificação , Weissella/fisiologia , Animais , Antibacterianos/farmacologia , Compostos de Bifenilo , Escherichia coli/crescimento & desenvolvimento , Cavalos , Concentração de Íons de Hidrogênio , Radical Hidroxila , Testes de Sensibilidade Microbiana , Viabilidade Microbiana/efeitos dos fármacos , Pasteurella/crescimento & desenvolvimento , Filogenia , Picratos , Salmonella/crescimento & desenvolvimento , Análise de Sequência de DNA , Staphylococcus aureus/crescimento & desenvolvimento , Estresse Fisiológico , Weissella/efeitos dos fármacos , Weissella/crescimento & desenvolvimento , alfa-Amilases/metabolismo
2.
Appl Environ Microbiol ; 76(4): 1008-13, 2010 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-20038698

RESUMO

Mannheimia (Pasteurella) haemolytica is the only pathogen that consistently causes severe bronchopneumonia and rapid death of bighorn sheep (BHS; Ovis canadensis) under experimental conditions. Paradoxically, Bibersteinia (Pasteurella) trehalosi and Pasteurella multocida have been isolated from BHS pneumonic lungs much more frequently than M. haemolytica. These observations suggest that there may be an interaction between these bacteria, and we hypothesized that B. trehalosi overgrows or otherwise inhibits the growth of M. haemolytica. Growth curves (monoculture) demonstrated that B. trehalosi has a shorter doubling time ( approximately 10 min versus approximately 27 min) and consistently achieves 3-log higher cell density (CFU/ml) compared to M. haemolytica. During coculture M. haemolytica growth was inhibited when B. trehalosi entered stationary phase (6 h) resulting in a final cell density for M. haemolytica that was 6 to 9 logs lower than expected with growth in the absence of B. trehalosi. Coculture supernatant failed to inhibit M. haemolytica growth on agar or in broth, indicating no obvious involvement of lytic phages, bacteriocins, or quorum-sensing systems. This observation was confirmed by limited growth inhibition of M. haemolytica when both pathogens were cultured in the same media but separated by a filter (0.4-microm pore size) that limited contact between the two bacterial populations. There was significant growth inhibition of M. haemolytica when the populations were separated by membranes with a pore size of 8 mum that allowed free contact. These observations demonstrate that B. trehalosi can both outgrow and inhibit M. haemolytica growth with the latter related to a proximity- or contact-dependent mechanism.


Assuntos
Mannheimia haemolytica/crescimento & desenvolvimento , Pasteurella/fisiologia , Animais , Técnicas Bacteriológicas , Sequência de Bases , Contagem de Colônia Microbiana , DNA Bacteriano/genética , Mannheimia haemolytica/genética , Mannheimia haemolytica/patogenicidade , Mannheimia haemolytica/fisiologia , Modelos Biológicos , Pasteurella/genética , Pasteurella/crescimento & desenvolvimento , Pasteurella/patogenicidade , Pasteurella multocida/crescimento & desenvolvimento , Pasteurella multocida/patogenicidade , Infecções por Pasteurellaceae/microbiologia , Infecções por Pasteurellaceae/veterinária , Pneumonia Bacteriana/microbiologia , Pneumonia Bacteriana/veterinária , Ovinos , Doenças dos Ovinos/microbiologia , Carneiro da Montanha
3.
J Zoo Wildl Med ; 40(1): 117-25, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19368250

RESUMO

Morbidity and mortality associated with respiratory disease following capture and translocation of bighorn sheep (Ovis canadensis canadensis) is a significant concern, particularly when establishing new or augmenting existing bighorn populations. Administration of prophylactic antibiotics at the time of capture is often done to minimize the risk of respiratory disease, but the efficacy of this practice is unknown. The effects of oxytetracycline and florfenicol on the Pasteurella (Bibersteinia) and Mannheimia spp. isolated from samples collected from the oropharynx at the time of capture and 3 or 42 day later were evaluated in two groups of bighorn sheep. The most evident change in the isolation rates or types of Pasteurella (Bibersteinia) spp., Mannheimia spp., or both was an increase of beta-hemolytic strains isolated from bighorn sheep 3 day following oxytetracycline treatment. Both groups of bighorn sheep carried Pasteurella (Bibersteinia) trehalosi identified as the same biovariants, but they did not share biovariants of Mannheimia spp. No animals had signs of respiratory disease. Isolates representative of all biovariants present in cultures from the two bighorn sheep groups were sensitive to in vitro tests to both oxytetracycline and florfenicol and the majority were also sensitive to seven other antibiotics tested. The administration of neither oxytetracycline nor florfenicol eliminated Pasteurella (Bibersteinia) or Mannheimia from the oropharyngeal mucosa. Resistance to either antibiotic used in these animals was not noted. Although the prophylactic benefits of these drugs in preventing disease are uncertain, therapeutic levels of antibiotics in lung tissue during times of stress may reduce the risk of disease. Representative sampling of the oropharyngeal microflora of bighorn sheep source and recipient populations prior to being intermingled should be considered as one of the tools to minimize exposure of naive populations to potentially pathogenic bacteria.


Assuntos
Antibacterianos/uso terapêutico , Infecções por Pasteurella/veterinária , Infecções por Pasteurellaceae/veterinária , Infecções Respiratórias/veterinária , Doenças dos Ovinos/epidemiologia , Carneiro da Montanha/microbiologia , Animais , Animais Selvagens/microbiologia , Animais de Zoológico , Antibacterianos/administração & dosagem , Antibioticoprofilaxia/veterinária , Conservação dos Recursos Naturais , Feminino , Masculino , Mannheimia/efeitos dos fármacos , Mannheimia/crescimento & desenvolvimento , Mannheimia/isolamento & purificação , Orofaringe/microbiologia , Pasteurella/efeitos dos fármacos , Pasteurella/crescimento & desenvolvimento , Pasteurella/isolamento & purificação , Infecções por Pasteurella/epidemiologia , Infecções por Pasteurella/mortalidade , Infecções por Pasteurella/prevenção & controle , Infecções por Pasteurellaceae/epidemiologia , Infecções por Pasteurellaceae/mortalidade , Infecções por Pasteurellaceae/prevenção & controle , Infecções Respiratórias/epidemiologia , Infecções Respiratórias/mortalidade , Infecções Respiratórias/prevenção & controle , Doenças dos Ovinos/mortalidade , Doenças dos Ovinos/prevenção & controle , Transportes
4.
FEMS Microbiol Lett ; 280(1): 42-9, 2008 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-18179587

RESUMO

Mannheimia haemolytica and Bibersteinia (Pasteurella) trehalosi are the most common bacterial isolates that cause pulmonary diseases in ruminants worldwide. The disease is determined by specific serotypes found in cattle and small ruminants. The molecular epidemiology of strains involved in disease is important in the control of outbreaks as well as in the preparation of vaccines. This study aimed to detect the instability and variations of bacterial strains that may affect the analysis of epidemic strains, or the stability of vaccinal strains. Eight strains of M. haemolytica belonging to serotypes A1 and A2 and three B. trehalosi strains of the T3 and T4 serotypes were used. Strains were subjected to pulsed field gel electrophoresis (PFGE) and capsular and phenotypic typing at each round of a total of 50 successive subcultures. Remarkable stability was found in all selected strains of B. trehalosi in contrast to M. haemoltyica, in which strains of both serotypes showed pattern variations produced by PFGE and capsular and phenotypic analysis. Objective criteria for M. haemolytica and B. trehalosi typing are consequently addressed.


Assuntos
Cápsulas Bacterianas/genética , Variação Genética , Mannheimia haemolytica/genética , Infecções por Pasteurella/veterinária , Pasteurella/genética , Animais , DNA Bacteriano/genética , Eletroforese em Gel de Campo Pulsado , Genótipo , Testes de Hemaglutinação , Mannheimia haemolytica/classificação , Mannheimia haemolytica/crescimento & desenvolvimento , Pasteurella/classificação , Pasteurella/crescimento & desenvolvimento , Infecções por Pasteurella/microbiologia , Fenótipo , Sorotipagem
5.
Pathol Biol (Paris) ; 49(8): 606-11, 2001 Oct.
Artigo em Francês | MEDLINE | ID: mdl-11692747

RESUMO

The MICs of seven quinolones, nalidixic acid, pefloxacin, ofloxacin, d-ofloxacin, ciprofloxacin, sparfloxacin and levofloxacin, were determined by agar dilution method comparatively to those of amoxycillin, cefpodoxime, doxycyclin and clarithromycin against 75 clinical isolates of Pasteurella multocida, P. dagmatis and P. canis. Time-kill method was performed for three selected P. multocida isolates. Fluoroquinolones were the most active agents. At concentration of 0.016 mg/L of sparfloxacin or levofloxacin the 75 isolates were inhibited. The MICs of levofloxacin and sparfloxacin showed that the activity of these molecules was two to four times higher than that of the other quinolones studied. Time-kill studies showed a complete killing in six hours with the CMI x 2 of pefloxacin, ofloxacin, ciprofloxacin, sparfloxacin and levofloxacin. This result was obtained more rapidly with the quinolones than with amoxicillin or cefpodoxime. Doxycycline and clarithromycin were devoid of bactericidal activity.


Assuntos
Anti-Infecciosos/farmacologia , Levofloxacino , Ofloxacino/farmacologia , Pasteurella/efeitos dos fármacos , Antibacterianos/administração & dosagem , Antibacterianos/farmacologia , Anti-Infecciosos/administração & dosagem , Humanos , Cinética , Testes de Sensibilidade Microbiana , Ofloxacino/administração & dosagem , Pasteurella/crescimento & desenvolvimento
6.
Lab Anim ; 35(2): 163-6, 2001 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11315166

RESUMO

Survival has been determined for Pasteurella pneumotropica on various surfaces found in an animal room at 23+/-1 degrees C and 50+/-10% relative humidity. Longest survival (120 min) was found on mouse hair, shortest (< 30 min) on laboratory coat fabric. Transmission experiments were performed using sentinel animals in order to evaluate the efficiency of their use for the detection of P. pneumotropica in quarantined mice. In sentinels exposed to infected mice by close contact, P. pneumotropica was detected by culture 2 weeks post-exposure and seroconversion 3 weeks after contact. Transfer of soiled bedding from Pasteurella-infected mice did not infect sentinels within a period of 12 weeks as tested by cultivation or serum antibodies.


Assuntos
Infecções por Pasteurella/veterinária , Pasteurella/crescimento & desenvolvimento , Doenças dos Roedores/transmissão , Animais , Anticorpos Antibacterianos/sangue , Vestuário , Transmissão de Doença Infecciosa/prevenção & controle , Transmissão de Doença Infecciosa/veterinária , Feminino , Cabelo/microbiologia , Abrigo para Animais , Camundongos , Camundongos Transgênicos , Infecções por Pasteurella/microbiologia , Infecções por Pasteurella/transmissão , Quarentena , Doenças dos Roedores/microbiologia , Organismos Livres de Patógenos Específicos
7.
J Appl Microbiol ; 85(4): 746-54, 1998 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-9812386

RESUMO

The findings from a biodegradability study of fluoranthene using two pure bacterial strains, Pasteurella sp. IFA (B-2) and Mycobacterium sp. PYR-1 (AM) are reported. Of total fluoranthene, 24% (B-2) and 46% (AM) was biodegraded in an aqueous medium during 14 d of incubation at room temperature. During this period the bacteria were capable of mineralizing approximately two-thirds (B-2) and four-fifths (AM) of biodegraded fluoranthene to CO2, while one-third (B-2) and one-fifth (AM) of the original fluoranthene remained as stable metabolic products. These metabolites were isolated using liquid-liquid extraction and identified using gas chromatography-mass spectrometry (GC-MS) and derivatization techniques. Two metabolites (9-fluorenone-1-carboxylic acid and 9-fluorenone) were identified by GC-MS directly, while the metabolites 9-fluorenone-1-carboxylic acid, 9-hydroxyfluorene, 9-hydroxy-1-fluorene-carboxylic acid, 2-carboxybenzaldehyde, benzoic acid and phenylacetic acid were determined in their derivatized forms. From the identified metabolites, a fluoranthene biodegradation pathway was proposed for Pasteurella sp. IFA.


Assuntos
Fluorenos/metabolismo , Mycobacterium/metabolismo , Pasteurella/metabolismo , Poluentes do Solo/metabolismo , Biodegradação Ambiental , Dióxido de Carbono/metabolismo , Cromatografia Gasosa , Meios de Cultura , Espectrometria de Massas , Mycobacterium/crescimento & desenvolvimento , Consumo de Oxigênio , Pasteurella/crescimento & desenvolvimento
8.
Ann N Y Acad Sci ; 849: 479-84, 1998 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-9668517

RESUMO

Pasteurella granulomatis (Pg) is a recently identified bacterium associated with proliferative fibrogranulomatous panniculitis (also called "lechiguana") in Brazilian cattle. Recent attempts to experimentally reproduce this disease have only been partially successful. We hypothesized that Pg may produce hemolysin(s) and/or cytotoxin(s) which could contribute to its pathogenicity in susceptible cattle. The objective of this study was to determine the presence and degree of hemolytic and leukotoxic activity of selected isolates of Pg. Either ovine or bovine blood agar plates were streaked with 1 of 7 Pg isolates, incubated at 37 degrees C +/- 1 C for 48 hours, and examined for hemolysis. Two of seven isolates showed hemolytic activity on bovine plates, while all seven showed hemolytic activity on ovine plates. By use of the CAMP reaction, involving simultaneous intersecting cultures of Staphylococcus aureus and Pg, all seven Pg isolates showed enhanced (positive CAMP) hemolysis within 24 hours on bovine blood agar plates. Preliminary results using tetrazolium (MTT) dye reductions with bovine neutrophils showed leukotoxicity in 13 of 16 Pg cultures. Alamar blue tests indicate leukotoxic activity for all 7 Pg isolates. We conclude that some Pg isolates have variable hemolytic and/or leukotoxic properties and that this variability (presence and/or degree) of these 2 properties may affect the relative pathogenicity of Pg in susceptible cattle.


Assuntos
Toxinas Bacterianas/biossíntese , Doenças dos Bovinos/microbiologia , Exotoxinas/biossíntese , Infecções por Pasteurella/veterinária , Pasteurella/fisiologia , Animais , Animais Recém-Nascidos , Sangue , Bovinos , Meios de Cultura , Hemólise , Pasteurella/crescimento & desenvolvimento , Pasteurella/isolamento & purificação , Infecções por Pasteurella/microbiologia , Ovinos
9.
Aust Vet J ; 75(2): 126-31, 1997 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-9066970

RESUMO

OBJECTIVE: To evaluate the administration of procaine penicillin prior to or during confinement with head elevation as a means of reducing the associated accumulation of inflammatory lower respiratory tract secretions and increased numbers of bacteria within the lower respiratory tract of confined horses. DESIGN AND PROCEDURE: Two experiments were conducted to evaluate the efficacy of different dose rates and dosing frequencies. In experiment A a single low dose (15,000 IU/kg) of procaine penicillin was administered to four horses immediately prior to confinement with head elevation for 48 hours. The systemic leucocyte response, gross and cytologic characteristics of transtracheal aspirate and bacterial numbers in lower respiratory tract samples were compared with corresponding samples from two horses confined with heads elevated but not given penicillin. The efficacy of higher dose rates (20,000 IU/kg and 40,000 IU/kg) given before and during confinement with heads elevated for 24 hours was evaluated in experiment B. RESULTS: Treatment with procaine penicillin had no effect on the systemic leucocyte response or on the accumulation of inflammatory lower respiratory tract secretions at any of the dosing schedules evaluated. The number of bacteria isolated from trans-tracheal samples was reduced at 12 hours for treated horses in experiment A and at 24 hours for experiment B. beta-haemolytic Streptococcus spp were not isolated from treated horses in either experiment. Bacterial species isolated from treated horses were predominantly Pasteurella and/or Actinobacillus spp, however, members of the family Enterobacteriaceae and a Staphylococcus sp were isolated from treated horses. One treated horse in experiment A developed clinically apparent pulmonary disease. CONCLUSIONS: The prophylactic administration of penicillin before or during confinement did not reliably reduce bacterial numbers or prevent the accumulation of purulent lower respiratory tract secretions in horses confined with their heads elevated. Numbers of beta-haemolytic Streptococcus spp were reduced following treatment, suggesting that the repeated administration of procaine penicillin may have some merit as part of a strategy to prevent transport-associated respiratory disease. However, methods directed at minimising the duration of confinement with head elevation, augmentation of the clearance of accumulated secretions and prompt identification of animals in which airway inflammation has extended to the pulmonary parenchyma remain the best ways of minimising transport-associated respiratory disease.


Assuntos
Doenças dos Cavalos/etiologia , Doenças dos Cavalos/prevenção & controle , Penicilina G Procaína/uso terapêutico , Penicilinas/uso terapêutico , Postura/fisiologia , Doenças Respiratórias/veterinária , Actinobacillus/crescimento & desenvolvimento , Actinobacillus/isolamento & purificação , Infecções por Actinobacillus/etiologia , Infecções por Actinobacillus/prevenção & controle , Infecções por Actinobacillus/veterinária , Animais , Relação Dose-Resposta a Droga , Enterobacteriaceae/crescimento & desenvolvimento , Enterobacteriaceae/isolamento & purificação , Infecções por Enterobacteriaceae/etiologia , Infecções por Enterobacteriaceae/prevenção & controle , Infecções por Enterobacteriaceae/veterinária , Feminino , Doenças dos Cavalos/fisiopatologia , Cavalos , Leucócitos/patologia , Pasteurella/crescimento & desenvolvimento , Pasteurella/isolamento & purificação , Infecções por Pasteurella/etiologia , Infecções por Pasteurella/prevenção & controle , Infecções por Pasteurella/veterinária , Sistema Respiratório/microbiologia , Doenças Respiratórias/etiologia , Doenças Respiratórias/prevenção & controle , Infecções Estafilocócicas/etiologia , Infecções Estafilocócicas/prevenção & controle , Infecções Estafilocócicas/veterinária , Staphylococcus/crescimento & desenvolvimento , Staphylococcus/isolamento & purificação , Infecções Estreptocócicas/etiologia , Infecções Estreptocócicas/prevenção & controle , Infecções Estreptocócicas/veterinária , Streptococcus/crescimento & desenvolvimento , Streptococcus/isolamento & purificação , Fatores de Tempo
10.
Antimicrob Agents Chemother ; 40(11): 2610-7, 1996 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-8913474

RESUMO

Siderophores selectively bind ferric iron and are involved in receptor-specific iron transport into bacteria. Several types of siderophores were synthesized, and growth-promoting or inhibitory activities when they were conjugated to carbacephalosporin, erythromycylamine, or nalidixic acid were investigated. Overall, 11 types of siderophores and 21 drug conjugates were tested against seven different bacterial species: Escherichia coli, Bordetella bronchiseptica, Pasteurella multocida, Pasteurella haemolytica, Streptococcus suis, Staphylococcus aureus, and Staphylococcus epidermidis. In some species, the inhibitory activities of the drug conjugates were associated with the ability of the bacteria to use the siderophore portion of the molecules for growth promotion in disc diffusion tests (0.04 mumol of conjugate or siderophore per disc). E. coli used catechol-based siderophore portions as well as hydroxamate-based tri-delta-OH-N-OH-delta-N-acetyl-L-ornithine ferric iron ligands for growth under iron-restricted conditions achieved by supplemental ethylenediamine di (O-hydroxyphenylacetic acid) (100 micrograms/ml) and was sensitive to carbacephalosporin conjugated to these siderophore types (up to a 34-mm-diameter inhibition zone). B. bronchiseptica used desferrioxamine B and an isocyanurate-based or trihydroxamate in addition to catechol-based siderophore portions for promotion but was not inhibited by beta-lactam conjugates partly because of the presence of beta-lactamase. P. multocida and P. haemolytica did not use any of the synthetic siderophores for growth promotion, and the inhibitory activities of some conjugates seemed partly linked to their ability to withhold iron from these bacteria, since individual siderophore portions showed some antibacterial effects. Individual siderophores did not promote S. suis growth in restrictive conditions, but the type of ferric iron ligands attached to beta-lactams affected inhibitory activities. The antibacterial activities of the intracellular-acting agents erythromycylamine and nalidixic acid were reduced or lost, even against S. aureus and S. epidermidis, when the agents were conjugated to siderophores. Conjugate-resistant E. coli mutants showed the absence of some iron-regulated outer membrane proteins in gel electrophoresis profiles and in specific phage or colicin sensitivity tests, implying that the drugs used outer membrane receptors of ferric complexes to get into cells.


Assuntos
Antibacterianos/metabolismo , Bactérias/metabolismo , Ferro/metabolismo , Sideróforos/metabolismo , Doenças dos Suínos/microbiologia , Animais , Antibacterianos/química , Bactérias/genética , Proteínas da Membrana Bacteriana Externa/metabolismo , Bordetella/genética , Bordetella/crescimento & desenvolvimento , Bordetella/metabolismo , Colicinas/farmacologia , Resistência Microbiana a Medicamentos , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Ácidos Hidroxâmicos/farmacologia , Testes de Sensibilidade Microbiana , Mutação , Pasteurella/genética , Pasteurella/crescimento & desenvolvimento , Pasteurella/metabolismo , Sideróforos/química , Streptococcus suis/genética , Streptococcus suis/crescimento & desenvolvimento , Streptococcus suis/metabolismo , Suínos
11.
Biochim Biophys Acta ; 1245(3): 407-13, 1995 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-8541320

RESUMO

A 78-kDa spermatozoa motility inhibiting factor (SMIF) was purified from chicken (Gallus domesticus) seminal plasma by anion exchange (DE-53) followed by affinity chromatography on concanavalin A-Sepharose. The factor is thermostable and inhibited the spermatozoa motility in a dose dependent manner. In addition, SMIF inhibited the growth of gram negative bacteria, Pasteurella multocida but not gram positive Streptococcus equi. The factor lost its spermatozoa immobilizing property after treatment with trypsin, chymotrypsin or pepsin. The inhibition of SMIF by beta-mercaptoethanol suggest the involvement of disulfide bonds in its activity. Similarly, this property was lost in presence of chicken seminal plasma or incubating SMIF with anti-SMIF antibodies. Evidence is provided for the presence of a high molecular weight protein (> 100 kDa) in chicken seminal plasma that neutralizes the motility inhibiting property of SMIF. No significant decrease in spermatozoa ATP was observed in presence of SMIF suggesting that the loss of spermatozoa motility was due to factors other than depletion in cell's energy. Using anti-SMIF antibodies, a cross-reactive protein was identified in the blood, liver and reproductive tissues of chicken and the seminal plasma of cattle and buffalo. However, the cross-reactive protein failed to inhibit chicken spermatozoa motility. The significance of SMIF in chicken seminal plasma is discussed.


Assuntos
Galinhas/metabolismo , Pasteurella/efeitos dos fármacos , Proteínas/isolamento & purificação , Sêmen/metabolismo , Motilidade Espermática/efeitos dos fármacos , Streptococcus/efeitos dos fármacos , Animais , Divisão Celular/efeitos dos fármacos , Inibição de Migração Celular , Masculino , Pasteurella/crescimento & desenvolvimento , Proteínas/farmacologia , Streptococcus/crescimento & desenvolvimento
12.
FEMS Microbiol Lett ; 124(3): 285-9, 1994 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-7531663

RESUMO

Pasteurella piscicida grown in a glucose-rich medium produces a capsule that can be see under light and electron microscopy. The capsular polysaccharide was purified and characterized by chemical and HPLC analysis. The polymer has the composition glucose/mannose/N-acetylgalactosamine/galacturonic acid/acetic acid in the molar ratios of approximately 2.5:1.3:0.5:0.4:2.5. The polysaccharide was immunogenic in rabbits and did not cross-react with antibodies against the O-antigen lipopolysaccharide.


Assuntos
Cápsulas Bacterianas/química , Pasteurella/química , Polissacarídeos Bacterianos/isolamento & purificação , Animais , Cápsulas Bacterianas/ultraestrutura , Cromatografia Líquida de Alta Pressão , Reações Cruzadas , Soros Imunes , Microscopia Eletrônica , Antígenos O , Pasteurella/crescimento & desenvolvimento , Pasteurella/imunologia , Polissacarídeos Bacterianos/imunologia , Coelhos
13.
Jikken Dobutsu ; 43(3): 375-9, 1994 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-7925625

RESUMO

Colonization pattern of Pasteurella pneumotropica (P. pneumotropica) in mice with latent pasteurellosis was examined with the original selective media, NKBT medium and TGN broth. In the mice of 0 to 15 weeks old, the organism was mainly isolated from the upper respiratory tract, lower intestinal tract, feces and vagina, with the highest isolation rate in the pharyngolarynx. In the pregnant and lactating mice, the organism was isolated from the same sites, but not from the uterus, fetus or mammary gland. In the neonates, the organism was isolated from the respiratory and intestinal tracts within 24hr after birth. The organism was constantly detected in the feces of the 0- to 20-week-old mice, with the highest viable count one week after birth. Through the monitoring of mouse colonies, the pharyngolarynx always showed higher isolation rate than the feces in several mouse strains. These results reveal that the pharyngolarynx is the primary colonization site of P. pneumotropica in mice, and the lower intestinal tract and vagina are also the main sites. Further, frequent isolation of the organism from the feces and vagina, and the results in neonates suggest the mode of the transmission to newborn mice in the colony, i.e., intravaginal infection at the partition, and oro-nasal infection through the maternal feces and saliva.


Assuntos
Infecções por Pasteurella/veterinária , Pasteurella/crescimento & desenvolvimento , Doenças dos Roedores/microbiologia , Animais , Animais de Laboratório , Animais Recém-Nascidos , Contagem de Colônia Microbiana , Feminino , Genitália/microbiologia , Intestinos/microbiologia , Masculino , Camundongos , Infecções por Pasteurella/microbiologia , Gravidez , Sistema Respiratório/microbiologia
14.
Avian Dis ; 38(2): 269-74, 1994 Apr-Jun.
Artigo em Inglês | MEDLINE | ID: mdl-7980274

RESUMO

The biochemical and serological properties of 29 isolates of avian haemophili obtained from chickens in Brazil are described. Twenty-seven of the isolates had the typical biochemical properties of Haemophilus paragallinarum. The two remaining isolates had the typical properties of Pasteurella avium, formerly known as Haemophilus avium. All of the H. paragallinarum isolates were serotyped according to the Page scheme using a hemagglutination-inhibition test. Fourteen of the isolates were serovar A, one was serovar B, 11 were serovar C, and one isolate could not be serotyped. The isolates were also examined using a panel of monoclonal antibodies for Page serovars A (one monoclonal antibody available) and C (three monoclonal antibodies available). As expected, the serovar B isolate failed to react with any monoclonal antibody, whereas the 11 serovar C isolates reacted with all three serovar C monoclonal antibodies but not with the serovar A monoclonal antibody. Only eight of the 14 serovar A isolates reacted with the serovar A monoclonal antibody.


Assuntos
Galinhas/microbiologia , Infecções por Haemophilus/veterinária , Haemophilus/isolamento & purificação , Infecções por Pasteurella/veterinária , Pasteurella/isolamento & purificação , Doenças das Aves Domésticas/microbiologia , Animais , Brasil , Demografia , Haemophilus/classificação , Haemophilus/crescimento & desenvolvimento , Infecções por Haemophilus/microbiologia , Pasteurella/classificação , Pasteurella/crescimento & desenvolvimento , Infecções por Pasteurella/microbiologia
16.
Equine Vet J ; 25(4): 314-8, 1993 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-8354218

RESUMO

The likelihood of finding evidence of inflammation in 551 tracheal washes collected endoscopically from 278 Thoroughbred racehorses increased with the number of bacterial colony forming units (cfu) per ml of wash (P < 0.001). The aerobic bacteria Streptococcus zooepidemicus, Pasteurella/Actinobacillus-like species and Streptococcus pneumoniae were significantly associated with lower airway inflammation whereas coagulase-negative Staphylococcus spp., alpha-haemolytic Streptococcus spp., Acinetobacter spp., Bacillus spp., Escherichia coli, Pseudomonas aeruginosa, non-haemolytic Streptococcus spp. and Enterobacteriaceae were not; Bordetella bronchiseptica was not isolated. Lower airway inflammation was particularly associated with bacteria in horses < or = 3 years of age. S. zooepidemicus, S. pneumoniae and Pasteurella/Actinobacillus-like species were isolated from 167 of 551 washes, either alone or in combination.


Assuntos
Doenças dos Cavalos/microbiologia , Infecções por Pasteurella/veterinária , Infecções Respiratórias/veterinária , Infecções Estreptocócicas/veterinária , Animais , Contagem de Colônia Microbiana , Doenças dos Cavalos/patologia , Cavalos , Inflamação , Pasteurella/crescimento & desenvolvimento , Pasteurella/isolamento & purificação , Infecções por Pasteurella/microbiologia , Infecções por Pasteurella/patologia , Infecções Respiratórias/microbiologia , Infecções Respiratórias/patologia , Infecções Estreptocócicas/microbiologia , Infecções Estreptocócicas/patologia , Streptococcus/crescimento & desenvolvimento , Streptococcus/isolamento & purificação , Traqueia/microbiologia , Traqueia/patologia
17.
J Vet Med Sci ; 55(3): 455-6, 1993 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-8357920

RESUMO

An acute death occurred in a racehorse with pneumonia after long-distance transportation in December, 1990. Pasteurella caballi, Streptococcus suis and Streptococcus zooepidemicus were isolated from the lung at high rate. Specific antigens of these bacteria were also demonstrated immunohistologically in the pneumonic lesion. These findings indicated that the disease is equine transport pneumonia caused by a mixed infection of the three bacterial species. This is the first report on the isolation of P. caballi and S. suis from a racehorse in Japan.


Assuntos
Doenças dos Cavalos , Infecções por Pasteurella/veterinária , Pneumonia/veterinária , Infecções Estreptocócicas/veterinária , Streptococcus suis , Animais , Encéfalo/microbiologia , Morte , Feminino , Coração/microbiologia , Cavalos , Pulmão/microbiologia , Linfonodos/microbiologia , Pasteurella/crescimento & desenvolvimento , Pasteurella/isolamento & purificação , Infecções por Pasteurella/complicações , Derrame Pleural/microbiologia , Pneumonia/microbiologia , Infecções Estreptocócicas/complicações , Streptococcus/isolamento & purificação , Streptococcus suis/isolamento & purificação
18.
Can J Vet Res ; 56(3): 177-83, 1992 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-1423052

RESUMO

Serial nasopharyngeal swab and bronchoalveolar lavage cultures were used to estimate changes in the bacterial flora of the respiratory tracts of calves during the first month after arrival in the feedlot. Bronchoalveolar lavage (BAL) differential cell counts served to evaluate pulmonary inflammatory changes during this period. Two groups of calves were studied, one consisting of clinically normal controls (n = 60), the other, of cases (n = 59) which received treatment for respiratory disease (penicillin +/- trimethoprimsulfadoxine). A variety of organisms, including Pasteurella multocida, Pasteurella haemolytica, Haemophilus somnus, Mycoplasma bovis and Mycoplasma bovirhinis, were present in the upper and lower airways of both groups during the postarrival period. With the exception of M. bovis, an overall decline in the prevalence of these organisms was observed during the course of the study. In cases, there was a marked decrease in the number of Pasteurella spp. and H. somnus isolates immediately following treatment. For the Pasteurella spp., however, this effect was shortlived as they often appeared to recolonize the respiratory tract within eight days of terminating antimicrobial therapy. Treatment did not appear to affect the frequency of isolating M. bovis. Its prevalence, in both groups of calves, increased to levels approaching 100% during the course of the study. All Pasteurella spp. isolates were tested for susceptibility to several commonly used antimicrobials. Resistance was only evident among P. haemolytica isolated from cases and in every instance this was to a combination of penicillin, ampicillin and tetracycline. Significantly more isolates were resistant after treatment than before. There were BAL differential cell count abnormalities indicative of inflammation in both cases and controls.(ABSTRACT TRUNCATED AT 250 WORDS)


Assuntos
Bactérias/crescimento & desenvolvimento , Infecções Bacterianas/veterinária , Doenças dos Bovinos/microbiologia , Sistema Respiratório/microbiologia , Infecções Respiratórias/veterinária , Animais , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Bactérias/efeitos dos fármacos , Infecções Bacterianas/tratamento farmacológico , Infecções Bacterianas/microbiologia , Líquido da Lavagem Broncoalveolar/citologia , Líquido da Lavagem Broncoalveolar/microbiologia , Bovinos , Doenças dos Bovinos/tratamento farmacológico , Contagem de Células/veterinária , Combinação de Medicamentos , Quimioterapia Combinada , Haemophilus/efeitos dos fármacos , Haemophilus/crescimento & desenvolvimento , Masculino , Mycoplasma/efeitos dos fármacos , Mycoplasma/crescimento & desenvolvimento , Nasofaringe/microbiologia , Pasteurella/efeitos dos fármacos , Pasteurella/crescimento & desenvolvimento , Penicilinas/farmacologia , Penicilinas/uso terapêutico , Recidiva , Infecções Respiratórias/tratamento farmacológico , Infecções Respiratórias/microbiologia , Sulfadoxina/uso terapêutico , Resultado do Tratamento , Trimetoprima/uso terapêutico
19.
Infect Immun ; 59(10): 3626-9, 1991 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-1894365

RESUMO

To study the pathogenesis of atrophic rhinitis, gnotobiotic pigs (n = 6) were inoculated intranasally with a sterile sonicate of a toxigenic strain of Bordetella bronchiseptica (0.16 mg of protein per ml) at 5 days of age, and they were then inoculated intranasally with 1 ml (5,250 CFU/ml) of a live, toxigenic strain of Pasteurella multocida at 7 days of age. Pigs were necropsied at 2, 5, 9, 14, 21, and 28 days postinoculation; those pigs necropsied after 5 days had developed turbinate atrophy. Other gnotobiotic pigs received the following inoculation protocols: (i) a sterile sonicate of a nontoxigenic strain of B. bronchiseptica (0.2 mg of protein per ml), followed by toxigenic P. multocida (n = 4); (ii) toxigenic P. multocida alone (n = 7); (iii) diluent (sterile tryptose broth) (n = 2); (iv) the sterile sonicate of toxigenic B. bronchiseptica alone (n = 2); or (v) the sterile sonicate of a nontoxigenic strain of B. bronchiseptica alone (n = 2). Turbinate atrophy did not occur in the latter groups except for one pig inoculated with only toxigenic P. multocida. These studies show that turbinate atrophy occurs in pigs given the toxigenic B. bronchiseptica sonicate and then given live, toxigenic P. multocida. This experimental regimen is a useful model for (i) studying the pathogenesis of atrophic rhinitis and (ii) testing vaccine strategies.


Assuntos
Bordetella/patogenicidade , Modelos Animais de Doenças , Pasteurella/patogenicidade , Rinite Atrófica/etiologia , Animais , Vida Livre de Germes , Pasteurella/crescimento & desenvolvimento , Pasteurella/isolamento & purificação , Rinite Atrófica/patologia , Suínos
20.
Mutat Res ; 263(3): 159-63, 1991 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-2067555

RESUMO

The response of Pasteurella haemolytica to ultraviolet irradiation was determined. The results for survival show that P. haemolytica is very sensitive to UV-irradiation. This UV-sensitivity is similar to E. coli strains defective in UV repair mechanism(s). Analysis of the distribution of TCA insoluble versus TCA soluble [3H]thymine dimers in UV-irradiated DNA of P. haemolytica during a 2-h post-irradiation period indicates that the bacterium is deficient in an excision-repair system. These data suggest that P. haemolytica lacks some of the important mechanisms to repair UV-induced damage.


Assuntos
Pasteurella/efeitos da radiação , Reparo do DNA , DNA Bacteriano/genética , DNA Bacteriano/efeitos da radiação , Cinética , Pasteurella/efeitos dos fármacos , Pasteurella/crescimento & desenvolvimento , Ácido Tricloroacético/farmacologia , Raios Ultravioleta
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