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1.
Food Chem ; 398: 133886, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-35963218

RESUMO

In this study, pectic oligosaccharides of orange peel (OPOs) were isolated and their structure characterized, and then screened according to anti-glycation level compared with aminoguanidine (AG). The results indicated that OPOs mainly included two components, and the main component has more than 50 % inhibition level for the seven glycation products at 60 °C for 40 h. At the accelerated storage temperature, OPOs demonstrated a better anti-glycation level than AG, and this inhibition was concentration-dependent. In addition, the main component in OPOs was separated into 10 fractions by DEAE Sephadex A-25 gel chromatography, the group of 5-7 monosaccharide polymerization showed the best anti-glycation effect, average anti-glycation capability on six products was over 70 % in the 40th hour. The anti-glycation level of this group was closely related to its high content of GalA and molar ratio of GalA: Rha, and positively correlated with time at moderate temperature.


Assuntos
Citrus sinensis , Pectinas , Monossacarídeos/análise , Oligossacarídeos , Pectinas/química , Temperatura
2.
Int J Food Microbiol ; 384: 109963, 2023 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-36274385

RESUMO

A mixed solid matrix of the depolymerized pectin (DP) and rice protein (RP) were investigated to improve the antibacterial activity of star anise essential oil (SAEO) through microencapsulation. The encapsulation was undertaken via electrostatic complexation method followed by spray drying. Under the optimized conditions, SAEO microcapsules with 92.2 % encapsulation efficiency was acquired at 3:2 of DP-to-RP, 1:3 of DP-to-SAEO and pH 4.0. DP-RP/SAEO microcapsules exhibited a spherical-shaped particle with smaller particle size, and sustained release. FTIR and morphology analysis confirmed that SAEO was successfully encapsulated in the solid matrix of microcapsules. DP-RP/SAEO microcapsules caused the destruction of cytomembranes and reduction of membrane proteins, which led to the alteration of cell membrane fluidity and integrity. Meanwhile, DP-RP/SAEO microcapsules repressed the key enzyme in tricarboxylic acid (TCA) and Hexose monophosphate pathway (HMP) cycle pathway of E. coli, S. aureus. The application experiments indicated DP-RP/SAEO microcapsules can effectively control the growth of E. coli and S. aureus in crab meatballs.


Assuntos
Braquiúros , Illicium , Óleos Voláteis , Oryza , Animais , Cápsulas , Pectinas/farmacologia , Staphylococcus aureus , Eletricidade Estática , Escherichia coli , Antibacterianos/farmacologia , Óleos Voláteis/farmacologia
3.
J Ethnopharmacol ; 301: 115862, 2023 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-36283638

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Panax ginseng C. A. Meyer (Ginseng) has traditionally been used to treat diabetes. Polysaccharide is the main active component of ginseng, and has been proved to have hypoglycaemic and hypolipidaemic effects, but its mechanism remains unclear. AIM OF THE STUDY: This study aimed to evaluate the effect and the potential mechanism of rhamnogalacturonan-I enriched pectin (GPS-1) from steamed ginseng on lipid metabolism in type 2 diabetes mellitus (T2DM) rats. MATERIALS AND METHODS: GPS-1 was prepared by water extraction, ion-exchange and gel chromatography. High-glucose/high-fat diet combined with streptozotocin was used to establish T2DM rat models, and lipid levels in serum and liver were tested. 16S rRNA sequencing and gas chromatography-mass spectrometry were used to detect the changes of gut microbiota and metabolites. The protein and mRNA levels of lipid synthesis-related genes were detected by Western blot and quantitative real-time polymerase chain reaction. RESULTS: The polyphagia, polydipsia, weight loss, hyperglycaemia, hyperlipidaemia and hepatic lipid accumulation in T2DM rats were alleviated after GPS-1 intervention. GPS-1 modulated the gut microbiota composition of T2DM rats, increased the levels of short-chain fatty acids, and promoted the secretion of glucagon-like peptide-1 and peptide tyrosine tyrosine. Further, GPS-1 activated AMP-activated protein kinases, phosphorylated acetyl-CoA carboxylase, reduced the expression of sterol regulatory element-binding protein-1c and fatty acid synthases in T2DM rats. CONCLUSIONS: The regulation effects of GPS-1 on lipid metabolism in T2DM rats are related to the regulation of gut microbiota and activation of AMP-activated protein kinase pathway.


Assuntos
Diabetes Mellitus Experimental , Diabetes Mellitus Tipo 2 , Microbioma Gastrointestinal , Panax , Ratos , Animais , Metabolismo dos Lipídeos , Panax/química , Proteínas Quinases Ativadas por AMP/metabolismo , Ramnogalacturonanos , Diabetes Mellitus Tipo 2/tratamento farmacológico , Diabetes Mellitus Tipo 2/metabolismo , Diabetes Mellitus Experimental/tratamento farmacológico , RNA Ribossômico 16S , Pectinas/farmacologia , Pectinas/metabolismo , Ácidos Graxos Voláteis , Tirosina/metabolismo
4.
Food Chem ; 402: 134229, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36182778

RESUMO

Multi-microscopy techniques and Fourier transform infrared (FT-IR) spectroscopy were used in this study to investigate the intrinsic causes leading to fruit texture difference between two cultivars of oriental melon 'HDB' (crisp) and 'HPM' (mealy). On the histological aspect, orderly arranged regular-shaped cells with tissue natural fracture pattern showed cell rupture in 'HDB' versus loosely arranged irregular-shaped cells with tissue natural fracture pattern showed cell-to-cell separation in 'HPM' of sarcocarp are histological causes for crisp and mealy fruit texture, respectively. On the biochemical aspect, FT-IR spectra (4000-850 cm-1) of sarcocarp tissue cell wall materials (CWM) happened a dramatic change at the mature stage in 'HPM', but not in 'HDB'. Insightly, the lower de-methyl-esterified homogalacturonan (HG) abundance with higher water-soluble pectin (WSP) ratio and lower hemicellulose (HC) content contribute a poor intercellular adhesion in 'HPM' middle lamella (ML) at the mature stage compared to 'HDB'.


Assuntos
Cucumis melo , Cucumis melo/química , Frutas/química , Espectroscopia de Infravermelho com Transformada de Fourier , Microscopia , Pectinas/química , Água/análise
5.
Food Chem ; 402: 134512, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36303394

RESUMO

In this work, the heat-induced ovalbumin (OVA)-pectin (PE) electrostatic complex particles (HIECP) prepared by different heating sequences (type I particles (I): Heat-treated ovalbumin/pectin complexes at pH 4; type II particles (II): Complexes between pre-heated ovalbumin and pectin at pH 4) and biopolymer ratios were used as stabilizers to form high internal phase Pickering emulsions (HIPPEs). The results showed that I had a more compact structure, higher net surface charge, and smaller particle size than II, due to the different growth nucleation mechanism. II-stabilized HIPPEs exhibited a smaller oil droplet size, stronger gel structure, and better stability than I-stabilized HIPPEs, owing to their suitable wettability, rigid "core-shell" structure, and robust and dense interface architecture. Moreover, the stability and gel-like structure of HIECP-stabilized HIPPEs improved with increasing PE content due to steric barrier and thickening effects. Our findings provide a new perspective for understanding heat-induced biopolymer particles as effective Pickering stabilizers.


Assuntos
Temperatura Alta , Pectinas , Emulsões/química , Eletricidade Estática , Ovalbumina , Tamanho da Partícula
6.
Food Chem ; 403: 134429, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36358075

RESUMO

Pure surimi of silver carp (Hypophthalmichthys molitrix) cannot meet the texture requirements in novel 3D printing of food. In this work, the effect mechanism of adding soluble pectin fiber on the quality of surimi was studied at molecular scale, and the interaction has been discussed by a novel prediction model. In comparison with pure surimi, pH and water-holding capacity decreased with increasing pectin, and texture properties improved. The storage modulus firstly rose and then decreased, reaching a maximum value of 15012 Pa at 0.5 % pectin addition. The thermal transition temperature of myosin was moved from 52.13 to 49.80 °C. Pectin extended the T22 relaxation time, suggesting a decrease in immobilized water. A new Time-series prediction model and interaction analysis further explored the intrinsic correlation of various parameters of surimi with different pectin additions. This work contributes to add our understanding of pectin application in surimi products in future special dietary food.


Assuntos
Carpas , Animais , Produtos Pesqueiros/análise , Pectinas , Géis/química , Água
7.
Food Chem ; 403: 134373, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36358094

RESUMO

To protect tomato pulp quality, this study investigated the effect of the infrared peeling method (using our newly developed catalytic infrared peeling equipment) on pectin's chemical, structural and functional properties and their correlation compared with manual, hot-water, and lye peeling methods. Infrared peeling significantly improved pectin's emulsifying and antioxidant capacity compared to manual peeling by increasing branching degree. Hot water peeling significantly improved pectin's viscosity, emulsifying and antioxidant capacity. However, the pectin chains had low flexibility. The effect of lye peeling on pectin was the greatest, causing the lowest linearity and the largest degree of branching. In comparison, infrared peeling had the least impact on pectin. It was further confirmed that pectin' viscosity, emulsifying, and antioxidant capacity were highly correlated with its chemical and structural properties. In summary, the infrared peeling method provides better pulp quality and is more sustainable because no water and chemicals are used.


Assuntos
Lycopersicon esculentum , Lixívia , Pectinas/química , Antioxidantes/química , Viscosidade
8.
J Sci Food Agric ; 103(1): 255-263, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-35859266

RESUMO

BACKGROUND: Knowledge about specific functional characteristics, such as viscosimetric, conductometric, tensiometric and structural properties of polysaccharide aqueous solutions is highly important in the successful and adequate application in food emulsion formulation. For the first time detailed characterization of sugar beet fibers aqueous solutions in comparison to high molecular weight (sugar beet pectin) and low molecular weight [octenyl succinic anhydride (OSA) maltodextrin] hydrocolloids/stabilizers was performed through viscometry, conductometry, tensiometry and component analysis. RESULTS: Sugar beet fibers and its water-soluble fraction were investigated. All sugar beet fiber samples showed substantial surface-active properties but different effect on the viscosity values of aqueous solutions. Sugar beet pectin had higher impact on aqueous solutions viscosity values compared to sugar beet fiber samples. Structural bonding between investigated polysaccharides were evaluated through conductometric measurements. Intermolecular linking and probable embedding of OSA maltodextrin molecules into the sugar beet fiber complex structure was detected in conductometric studies. The increased concentration of sugar beet fibers in the presence of sugar beet pectin led to the accelerated increase in specific conductivity values indicating effects of 'macromolecular crowding', intermolecular and intramolecular conformation changes and charge formation. CONCLUSIONS: Detailed characterization of sugar beet fibers provided scientific insight towards fundamental characteristics of sugar beet fiber aqueous solutions. The presented characteristics are particularly applicable in the field of food emulsion stabilization due to the presented surface-active properties of sugar beet fibers as well as specific characteristics of investigated multi-polysaccharide systems. © 2022 Society of Chemical Industry.


Assuntos
Beta vulgaris , Condutometria , Pectinas/química , Beta vulgaris/química , Anidridos Succínicos , Emulsões/química , Água/química , Fibras na Dieta , Açúcares
9.
Food Chem ; 401: 134117, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36096001

RESUMO

Although nanocomposite films had shown excellent potential in antibacterial food packaging, their potential harmful impact limits their further application in reality. Therefore, exploring a Generally Recognized As Safe (GRAS) nanomaterial that has antibacterial ability is the pioneer for the fabrication of a real edible nanocomposite-based antibacterial packaging film. Herein, for the first time by using the natural nanostructure extracted from cuttlefish ink, an edible antibacterial food packaging with high safety were constructed. The natural melanin nanoparticles (NMPs) in cuttlefish ink have good photothermal conversion ability. As such, by incorporating with natural pectin (PC) film and with near infrared (NIR) irradiation triggering, the results show that PC/NMPs films perform high-efficiency and short-term bactericidal activity against foodborne pathogenic bacteria, including thermotolerant Listeria monocytogenes. The sterilization rate could reach more than 90 % within only 5 min. Also, this nanocomposite film showed better mechanical properties, thermal stability and barrier properties than the neat pectin film. Antibacterial test on food sample also proved the good antibacterial ability of the PC/NMPs films. Therefore, exploring GRAS natural functional nanocomposite film is expected to be the effective way to promote edible nano-antibacterial packaging.


Assuntos
Filmes Comestíveis , Nanopartículas , Embalagem de Alimentos/métodos , Melaninas , Antibacterianos/farmacologia , Antibacterianos/química , Pectinas/farmacologia , Pectinas/química
10.
Food Chem ; 401: 134156, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36099826

RESUMO

Influences of conventional thermal and innovative non-thermal extraction methods on the physicochemical characteristics and properties of pectic polysaccharides from Choerospondias axillaris peels were investigated. Results showed that ultrasound-assisted extracted polysaccharides (UP) had a heterogeneous nature with lower molecular weight (127.7 kDa) and lower neutral sugar content (35.1%) but higher contents of protein (4.8%) and phenolic compounds (5.1%) than those of polysaccharides extracted by hot water (HP). Additionally, the monosaccharide composition results showed that glucose (77.8%) was the most abundant monosaccharide in HP, while arabinose (67.1%) was the most abundant monosaccharide in UP. The ultrasound significantly induced the degradation of polysaccharide chains but reduced the thermal degradation of phenolics. Finally, we found that UP had higher apparent viscosity, interfacial, emulsifying and antioxidant activity but lower α-glucosidase inhibition activity than those of HP. The results indicated that we could obtain polysaccharides with different functional and biological properties by using different extraction methods.


Assuntos
Anacardiaceae , Pectinas , Pectinas/química , Antioxidantes/química , alfa-Glucosidases/metabolismo , Arabinose , Anacardiaceae/química , Polissacarídeos/química , Monossacarídeos , Água/química , Fenóis , Glucose
11.
Food Chem ; 401: 134079, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36115226

RESUMO

Due to the hydrophilic of the pectin material, the coating has poor barrier properties and a negative preservation effect on fresh fruits. In this study, citrus pectin coating with improved barrier and antioxidant properties was prepared by embedding with functional cellulose nanocrystals (CNC). It was assessed that cellulose nanocrystals grafted with p-coumaric acid (CNC-P) were uniformly dispersed in the pectin matrix to improve coating barrier properties. The addition of 8 % CNC-P to the pectin coating led to a decrease in water vapor and oxygen permeability from the coating by 12.6 % and 22.3 %, respectively. Additionally, the grafted p-coumaric acid (PA) introduced antioxidant properties to the cellulose nanocrystals. The fresh-cut fruits preservation assay showed that the coating containing CNC-P exerted a stronger inhibition effect of the browning process within 8 h than other coatings. This study suggests that pectin coating embedded with CNC-P has the potential to be used in food packaging.


Assuntos
Nanocompostos , Nanopartículas , Celulose/química , Antioxidantes , Vapor , Nanocompostos/química , Pectinas/química , Nanopartículas/química , Oxigênio
12.
Carbohydr Polym ; 300: 120272, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36372494

RESUMO

The aim of this study was to design a pectin-chitosan (PEC-CS) hydrogel loaded with a bioadhesive-design micelle containing large amount of ciprofloxacin for antibacterial and healing wound applications. Pectin and chitosan are crosslinked in a safe and convenient way, and the PEC-CS hydrogel have high water content (>95 %), strong water absorption (15,000 %), good water retention (>10,000 % at 30 % RH for 12 h), and the PEC-CS hydrogels showed no cytotoxicity and hemolysis, thus providing a humid microenvironment suitable for wound. Additionally, the dopamine modified carrier can greatly improve the solubility and retention time in the wound of ciprofloxacin, effectively increase the efficiency of drug loading into the PEC-CS hydrogels and exert antibacterial activity in the wound for a long time. In vitro and in vivo pharmacodynamics experiments have shown that PEC-CS#CIP@DPDMCs hydrogels can resist bacteria and promote wound healing. Thus,The PEC-CS#CIP@DPDMCs hydrogels can be a potential anti-infective hydrogel excipient.


Assuntos
Infecções Bacterianas , Quitosana , Humanos , Hidrogéis/farmacologia , Pectinas/farmacologia , Micelas , Cicatrização , Ciprofloxacina , Antibacterianos/farmacologia , Água
13.
Methods Mol Biol ; 2566: 243-259, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36152257

RESUMO

Autofluorescence of plant tissues can be used as a label-free method to detect a range of phenolic-based cell wall components including lignin, suberin, and ferulate using widefield or confocal fluorescence microscopy. Likewise, fluorescently labeled antibodies can be used to localize specific carbohydrate molecules including arabinoxylan, ß-1,4 galactan, glucomannan, glucuronoxylan, pectins, and xyloglucan. When combined, these two methods allow detailed study of topochemistry in different plant tissues for phenotyping of mutant varieties and plant biology studies. This article describes the protocols for fluorescent detection and imaging of molecules in plant cell walls using autofluorescence and immunofluorescence.


Assuntos
Parede Celular , Lignina , Parede Celular/química , Galactanos , Lignina/química , Microscopia de Fluorescência/métodos , Pectinas/análise
14.
Methods Mol Biol ; 2566: 269-279, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36152259

RESUMO

The plant cell wall comprises various types of macromolecules whose abundance and spatial distribution change dynamically and are crucial for plant architecture. High-resolution live cell imaging of plant cell wall components is, therefore, a powerful tool for plant cell biology and plant developmental biology. To acquire suitable data, the experimental setup for staining and imaging of non-fixed samples must be straightforward and avoid creating stress-induced artifacts. We present a detailed sample preparation and live image acquisition protocol for fluorescence visualization of cell wall components using commercially available probes and stains.


Assuntos
Celulose , Pectinas , Membrana Celular/metabolismo , Parede Celular/metabolismo , Celulose/metabolismo , Pectinas/metabolismo , Células Vegetais/metabolismo
15.
Food Chem ; 402: 134253, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36162172

RESUMO

In this study, shell-core fibers were successfully prepared by using Eudragit S100 (ES100) and poly(vinyl alcohol) (PVA)/pectin (PEC) through coaxial electrospinning technology. The electrospun fiber was characterized by attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD). Thermo-gravimetric analysis (TGA) showed that the coaxial electrospun fiber encapsulated with Lactobacillus rhamnosus 1.0320 (L. rhamnosus) had higher thermal stability than the electrospun fiber prepared by uniaxial electrospinning. L. rhamnosus encapsulated by coaxial electrospun fiber maintained 90.07% and 91.96% survivability in simulated gastric and intestinal fluids. After continuous simulated gastrointestinal fluid treatment, the survival rate of L. rhamnosus encapsulated by coaxial electrospun fiber was 81.40%. The results indicate that shell-core fiber-encapsulated probiotics can improve the tolerance of probiotics to the harsh environment of gastrointestinal tract. The fiber prepared in this study can be applied to the preparation of functional fermented food such as probiotic yogurt fermentation in the future.


Assuntos
Lactobacillus rhamnosus , Nanofibras , Probióticos , Álcool de Polivinil/química , Fibras na Dieta , Probióticos/química , Pectinas , Nanofibras/química
16.
Food Chem ; 403: 134378, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36166923

RESUMO

Rhamnogalacturonan type I (RG-I) is one of the pectin family member abundant in plant cell walls. Process of RG-I extraction from cell walls, either as a one-step or several-stage process, conditions the structure and properties of obtained polysaccharides. In this paper, we provide comprehensive overview of the factors related to the source and extraction techniques that determine the yield and chemical composition of pectin belonging to RG-I. The role of the source material, solvent, pH, temperature, time and additional factors related to applied techniques, such as microwaves, ultrasounds, high and low pressure or enzymatic treatments are discussed.


Assuntos
Parede Celular , Pectinas , Pectinas/química , Parede Celular/química , Polissacarídeos/análise , Micro-Ondas
17.
Food Chem ; 399: 133997, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36037687

RESUMO

The effect of hydrogen-rich water (HRW) treatment on softening, cell wall components and cell wall metabolic genes in okras after harvest was studied. The results showed that HRW treatment could maintain fruit firmness and delay softening, thereby prolonging shelf life in okras during storage. The treated okras displayed significantly lower levels water- and chelate-soluble pectins while higher contents of Na2CO3-soluble pectin, hemicellulose and cellulose. The cell wall biosynthesis was maintained by HRW treatment via up-regulating genes involved in biosynthesis of pectin, hemicellulose and cellulose at the beginning of storage. On the contrary, the treatment could inhibit the cell wall disassembly due to the down-regulation of numerous cell wall degradative genes including AePME, AeGAL and AeCX at the end of storage. Taken together, our results suggested that HRW treatment delayed softening and extended shelf life in postharvest okras through modifying cell wall biosynthesis and disassembly at different times of storage.


Assuntos
Abelmoschus , Frutas , Abelmoschus/metabolismo , Parede Celular/metabolismo , Celulose/metabolismo , Frutas/metabolismo , Hidrogênio/farmacologia , Pectinas/metabolismo , Água/metabolismo
18.
Food Chem ; 398: 133801, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-35961168

RESUMO

Effects of pectin, inulin, and their combination on the production of microbiota-derived indoles and short-chain fatty acids (SCFAs) from different colon segments were investigated in a batch system inoculated with microbiota from proximal colon (PC) and distal colon (DC) compartments of the Simulator of Human Intestinal Microbial Ecosystem. Bacteria from DC compartment had a higher abundance of Firmicutes and a stronger capacity to produce indoles and SCFAs than bacteria from PC compartment. Fiber supplementation significantly increased the production of SCFAs, indole-3-propionic acid, and indole-3-lactic acid, but decreased the production of oxindole, tryptamine, and serotonin. Pectin specifically promoted the production of indole-3-acetic acid and indole-3-aldehyde. Interestingly, supplementation of pectin or inulin increased the relative abundance of Bacteroidetes whereas supplementation of a mixture of two fibers decreased it. Overall, these results suggest that fiber supplementation and colon segment affect the composition of gut microbiota and the microbial catabolism of tryptophan.


Assuntos
Inulina , Microbiota , Bactérias/genética , Bactérias/metabolismo , Colo/metabolismo , Fibras na Dieta/metabolismo , Ácidos Graxos Voláteis/metabolismo , Fermentação , Humanos , Indóis/metabolismo , Inulina/metabolismo , Pectinas/metabolismo
19.
Sci Rep ; 12(1): 19834, 2022 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-36400863

RESUMO

A 23 factorial design was used to evaluate the influence of temperature, catalyst and time and esterification degree (DE) of pectin obtained from mango, orange and tangerine peels as well as tamarind seeds by using the acid hydrolysis method. The study showed that a high temperature positively influenced the percentage of pectin yield for the four second generation biomasses. Nevertheless, the temperature showed a greater influence in the solubility and diffusion of the acid solvent in the tamarind seed matrix, resulting a pectin recovery 32.9%. Concerning the %DE, the most statistically significant value observed was dependent on the type of biomass studied. The %DE and the nature of the pectin are determining factors in the pectin's final use, in the present work the pectin extracted was used to produce furfural, a precursor of high value chemicals. The furfural production was achieved through alkaline hydrolysis and enhanced using the Maillard reaction, reaching a maximum concentration of 71.8 g/L which represents a 42.1% increase from the alkaline hydrolysis.


Assuntos
Mangifera , Pectinas , Reação de Maillard , Furaldeído , Esterificação
20.
Environ Health Perspect ; 130(11): 117003, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36331819

RESUMO

BACKGROUND: Perfluorooctane sulfonate (PFOS) is a persistent environmental pollutant that has become a significant concern around the world. Exposure to PFOS may alter gut microbiota and liver metabolic homeostasis in mammals, thereby increasing the risk of cardiometabolic diseases. Diets high in soluble fibers can ameliorate metabolic disease risks. OBJECTIVES: We aimed to test the hypothesis that soluble fibers (inulin or pectin) could modulate the adverse metabolic effects of PFOS by affecting microbe-liver metabolism and interactions. METHODS: Male C57BL/6J mice were fed an isocaloric diet containing different fibers: a) inulin (soluble), b) pectin (soluble), or c) cellulose (control, insoluble). The mice were exposed to PFOS in drinking water (3µg/g per day) for 7 wk. Multi-omics was used to analyze mouse liver and cecum contents. RESULTS: In PFOS-exposed mice, the number of differentially expressed genes associated with atherogenesis and hepatic hyperlipidemia were lower in those that were fed soluble fiber than those fed insoluble fiber. Shotgun metagenomics showed that inulin and pectin protected against differences in microbiome community in PFOS-exposed vs. control mice. It was found that the plasma PFOS levels were lower in inulin-fed mice, and there was a trend of lower liver accumulation of PFOS in soluble fiber-fed mice compared with the control group. Soluble fiber intake ameliorated the effects of PFOS on host hepatic metabolism gene expression and cecal content microbiome structure. DISCUSSIONS: Results from metabolomic, lipidomic, and transcriptomic studies suggest that inulin- and pectin-fed mice were less susceptible to PFOS-induced liver metabolic disturbance, hepatic lipid accumulation, and transcriptional changes compared with control diet-fed mice. Our study advances the understanding of interaction between microbes and host under the influences of environmental pollutants and nutrients. The results provide new insights into the microbe-liver metabolic network and the protection against environmental pollutant-induced metabolic diseases by high-fiber diets. https://doi.org/10.1289/EHP11360.


Assuntos
Poluentes Ambientais , Inulina , Camundongos , Masculino , Animais , Inulina/metabolismo , Inulina/farmacologia , Lipidômica , Metagenômica , Transcriptoma , Camundongos Endogâmicos C57BL , Fígado , Poluentes Ambientais/metabolismo , Fibras na Dieta/metabolismo , Fibras na Dieta/farmacologia , Pectinas/metabolismo , Pectinas/farmacologia , Mamíferos
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