Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 968
Filtrar
1.
Carbohydr Polym ; 303: 120452, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36657842

RESUMO

The pollen of Typha angustifolia L. decoction was clinically used to treat hyperlipidemia in China. A pectin polysaccharide (PTPS-2-2) was obtained from T. angustifolia pollen through water extraction, ion-exchange chromatography, and gel chromatography. Structural characterization showed that PTPS-2-2 had a molecular weight of 54 kDa and was composed of rhamnose, arabinose, xylose, galactose, and galacturonic acid with a molar ratio of 11.5: 36.5: 4.1: 36.7: 11.2. PTPS-2-2 consisted of rhamnogalacturonan I (RG-I) and arabinogalactan II (AG-II) domains. Its backbone was predominantly composed of →4-α-D-GalpA-(1 â†’ 2)-α-L-Rhap-(1→, with branches of 1,3-Galp, 1,6-Galp, 1,3,6-Galp, T-Araf, 1.5-Araf and T-Xylp, connected to the 4-position of 1,2-Rhap and the 3-position of 1,4-GalpA. The inhibitory effect of PTPS-2-2 on lipid accumulation was studied in vitro, using L02 cells induced by oleic acid. This experiment shows that PTPS-2-2 treatment at 100-400 µg/mL dose-dependently reduce cellular triglycerides (TG), cholesterol (TC), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and malondialdehyde (MDA) levels, while elevated superoxide dismutase (SOD) levels. This indicated that PTPS-2-2 potentially ameliorated oleic acid-induced hepatic steatosis by inhibiting lipid accumulation and oxidative stress.


Assuntos
Ácido Oleico , Typhaceae , Pectinas/farmacologia , Pectinas/química , Polissacarídeos/farmacologia , Polissacarídeos/química , Galactose/química
2.
Carbohydr Polym ; 303: 120467, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36657846

RESUMO

Pectic polysaccharide has attracted increasing attention for their potential biological properties and applications in health industries. In this study, a low-molecular-weight pectic polysaccharide, POS4, was obtained from citrus peel. The structure of POS4 was preliminarily analyzed by gel-permeation chromatography, monosaccharide analysis, infrared spectroscopy (IR) and nuclear magnetic resonance spectroscopy (NMR). Results showed that the molecular weight of POS4 was 4.76 kDa and it was a galacturonic acid enriched pectic polysaccharide. The anti-aging activity in vivo showed that POS4 could notably prolong the average lifespan of fruit flies by suppressing the generation of reactive oxygen species (ROS). Further studies demonstrated that POS4 could enhance intestinal homeostasis by modulating gut microbiota in a positive way and regulating autophagy associated genes. Taken together, we proposed that galacturonic acid enriched low molecular weight pectic polysaccharide have great potential in the development of healthy foods such as anti-aging health care products.


Assuntos
Pectinas , Polissacarídeos , Pectinas/farmacologia , Pectinas/química , Peso Molecular , Polissacarídeos/farmacologia , Polissacarídeos/química
3.
Carbohydr Polym ; 303: 120432, 2023 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-36657866

RESUMO

To study structure-function relationships of pectic polysaccharides with their immunostimulatory activity, broccoli by-products were used. Pectic polysaccharides composed by 64 mol% uronic acids, 18 mol% Ara, and 10 mol% Gal, obtained by hot water extraction, activated B lymphocytes in vitro (25-250 µg/mL). To disclose active structural features, combinations of ethanol and chromatographic fractionation and modification of the polysaccharides were performed. Polysaccharides insoluble in 80 % ethanol (Et80) showed higher immunostimulatory activity than the pristine mixture, which was independent of molecular weight range (12-400 kDa) and removal of terminal or short Ara side chains. Chemical sulfation did not promote B lymphocyte activation. However, the action of pectin methylesterase and endo-polygalacturonase on hot water extracted polysaccharides produced an acidic fraction with a high immunostimulatory activity. The de-esterified homogalacturonan region seem to be an important core to confer pectic polysaccharides immunostimulatory activity. Therefore, agri-food by-products are a source of pectic polysaccharide functional food ingredients.


Assuntos
Brassica , Ativação Linfocitária , Polissacarídeos/química , Pectinas/farmacologia , Pectinas/química , Linfócitos B , Água/química , Relação Estrutura-Atividade
4.
Int J Biol Macromol ; 227: 241-251, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36539172

RESUMO

In this paper, a kind of superabsorbent resin (SAR) with superior quality for hygiene products was developed using Fructus Aurantii Immaturus pectin (FAIP) from Citrus aurantium L.. FAIP-g-AM/AMPS SAR was established by free radical graft co-polymerization with FAIP as skeleton structure, N, N'-Methylene-bis (acrylamide) (MBA) as the cross-linker. Meanwhile, the functional monomers of acrylamide (AM) and 2-acrylamido-2-methylpropane sulfonic acid (AMPS) were introduced. The structure and morphology of FAIP-g-AM/AMPS were characterized by FTIR, 13C NMR, XRD, SEM and TG-DSC analysis. The results confirmed that the AFIP-g-AM/AMPS SAR was successfully prepared, which exhibited a three-dimensional (3D) network structure and an excellent thermal stability. The absorption and retention capacity of FAIP-g-AM/AMPS was comparable to or even better than commercial diapers and sanitary napkins. Significantly, FAIP-g-AM/AMPS itself exhibited excellent antibacterial and safety. FAIP-g-AM/AMPS has an inhibition ratio of 97.1 % for Escherichia coli (E. coli) and 98.5 % for Staphylococcus aureus (S. aureus), and was non-irritating and non-allergic to the skin. In addition, FAIP-g-AM/AMPS presented amazing biodegradability and a weight loss reached 37.1 % after 30 days by soil burial test. The research provides a safe and high-performance SAR, which expected to be used in hygiene products such as baby diapers, adult incontinence pads and sanitary napkins.


Assuntos
Pectinas , Staphylococcus aureus , Pectinas/farmacologia , Escherichia coli , Antibacterianos/farmacologia , Antibacterianos/química , Acrilamidas/química , Cloreto de Sódio , Acrilamida
5.
Int J Mol Sci ; 23(23)2022 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-36499122

RESUMO

The study aimed to compare the in vitro biocompatibility of pectin gels formed by different cross-linking cations. Hydrogel beads named CaPG, ZnPG, FePG, and AlPG were prepared from 4% solutions of apple pectin using ionotropic gelling with CaCl2, ZnCl2, FeCl3, and AlCl3, respectively. Cations influenced the gel strength of the wet gel beads in the following order (least strong) Ca2+ < Zn2+ < Fe3+~Al3+ (most strong). The swelling degree of the CaPG beads after 24 h of incubation in the RPMI-1640 medium was 104%, whereas the ZnPG, FePG, and AlPG beads swelled by 76, 108, and 134%, respectively. The strength of the pectin gel decreased significantly after incubation in the RPMI-1640 medium for 24 h, regardless of the cross-linking cation, although the FePG beads remained the strongest. All the pectin beads adsorbed serum proteins to a low degree, however the serum protein adsorption by the ZnPG and FePG beads (1.46 ± 0.87 and 1.35 ± 0.19 µg/mm2) was more than the CaPG and AlPG beads (0.31 ± 0.36 and 0.44 ± 0.25 µg/mm2). All the pectin beads reduced the production of TNF-α and IL-10 by hPBMCs in response to LPS stimulation. The IL-1ß response of cells to LPS was significantly reduced by the CaPG, ZnPG, and FePG beads, whereas the AlPG beads enhanced it twofold. The CaPG, FePG, and AlPG beads had no cytotoxicity. The viability of hPBMCs and human fibroblasts incubated with ZnPG beads was 5.3 and 7.2%, respectively. Thus, the use of different cross-linking cations changed the properties of the pectin gel, which is important for biocompatibility.


Assuntos
Pectinas , Humanos , Géis , Pectinas/farmacologia , Cátions
6.
Molecules ; 27(23)2022 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-36500604

RESUMO

For the food sector, onion rejects are an appealing source of value-added byproducts. Bioactive compounds were recovered from yellow onion rejects using a pulse electric field process at 6000 v and 60 pulses. The onion extract was encapsulated with whey protein isolate (WPI), pectin (P), and sodium caseinate (SC) with a mass ratio of 1:5 (extract/wall material, w/w). A Simplex lattice with augmented axial points in the mixture design was applied for the optimization of wall material for the encapsulation of onion reject extract by freeze-drying (FD). The optimal wall materials were 47.6 g/100 g (SC), 10.0 g/100 g (P), and 42.4 g/100 g (WPI), with encapsulation yield (EY) of 85.1%, total phenolic content (TPC) of 48.7 mg gallic acid equivalent/g DW, total flavonoid content (TFC) of 92.0 mg quercetin equivalent/g DW, and DPPH capacity of 76.1%, respectively. The morphological properties of the optimal encapsulate demonstrated spherical particles with a rough surface. At optimal conditions, the minimum inhibitory concentration (MIC) of the extract (mean diameter of inhibition zone: 18.8 mm) was shown as antifungal activity against Aspergillus&nbsp;niger.


Assuntos
Caseínas , Pectinas , Pectinas/farmacologia , Pectinas/química , Proteínas do Soro do Leite/química , Cebolas , Cápsulas/química
7.
Molecules ; 27(23)2022 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-36500662

RESUMO

A novel pectic polysaccharide (HPP-1) with high immunomodulatory activity was extracted and isolated from the immature honey pomelo fruit (Citrus grandis). Characterization of its chemical structure indicated that HPP-1 had a molecular weight of 59,024 D. In addition, HPP-1 was primarily composed of rhamnose, arabinose, fucose, mannose, and galactose at a molar ratio of 1.00:11.12:2.26:0.56:6.40. Fourier-transform infrared spectroscopy, periodic acid oxidation, and Smith degradation results showed that HPP-1 had α- and ß-glycosidic linkages and 1 → 2, 1 → 4, 1 → 6, and 1 → 3 glycosidic bonds. 13C NMR and 1H NMR analyses revealed that the main glycogroups included 1,4-D-GalA, 1,6-ß-D-Gal, 1,6-ß-D-Man, 1,3-α-L-Ara, and 1,2-α-L-Rha. Immunomodulatory bioactivity analysis using a macrophage RAW264.7 model in vitro revealed that NO, TNF-α, and IL-6 secretions were all considerably increased by HPP-1. Moreover, RT-PCR results showed that HPP-1-induced iNOS, TNF-α, and IL-6 expression was significantly increased in macrophages. HPP-1-mediated activation in macrophages was due to the stimulation of the NF-κB and MAPK signaling pathways based on western blot analyses. HPP-1 extracted from immature honey pomelo fruit has potential applications as an immunomodulatory supplement.


Assuntos
Frutas , Pectinas , Camundongos , Animais , Pectinas/farmacologia , Pectinas/análise , Frutas/química , Fator de Necrose Tumoral alfa/metabolismo , Células RAW 264.7 , Interleucina-6/metabolismo , Fatores Imunológicos/química , Polissacarídeos/química
8.
Nutrients ; 14(23)2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36501138

RESUMO

Obesity and type II diabetes are closely related to the rapid digestion of starch. Starch is the major food-energy source for most humans, and thus knowledge about the regulation of starch digestion can contribute to prevention and improved treatment of carbohydrate metabolic disorders such as diabetes. Pectins are plant polysaccharides with complex molecular structures and ubiquitous presence in food, and have diverse effects on starch digestion. Pectins can favorably regulate in vivo starch digestion and blood glucose level responses, and these effects are attributed to several reasons: increasing the viscosity of digesta, inhibiting amylase activity, and regulating some in vivo physiological responses. Pectins can influence starch digestion via multiple mechanisms simultaneously, in ways that are highly structure-dependent. Utilizing the multi-functionalities of pectin could provide more ways to design low glycemic-response food and while avoiding the unpalatable high viscosity in food by which is commonly caused by many other dietary fibers.


Assuntos
Diabetes Mellitus Tipo 2 , Amido , Humanos , Amido/metabolismo , Pectinas/farmacologia , Digestão , Fibras na Dieta/farmacologia
9.
Molecules ; 27(21)2022 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-36364232

RESUMO

Pectin is an acidic heteropolysaccharide found in the cell walls and the primary and middle lamella of land plants. To be authorized as a food additive, industrial pectins must meet strict guidelines set forth by the Food and Agricultural Organization and must contain at least 65% polygalacturonic acid to achieve the E440 level. Fruit pectin derived from oranges or apples is commonly used in the food industry to gel or thicken foods and to stabilize acid-based milk beverages. It is a naturally occurring component and can be ingested by dietary consumption of fruit and vegetables. Preventing long-term chronic diseases like diabetes and heart disease is an important role of dietary carbohydrates. Colon and breast cancer are among the diseases for which data suggest that modified pectin (MP), specifically modified citrus pectin (MCP), has beneficial effects on the development and spread of malignancies, in addition to its benefits as a soluble dietary fiber. Cellular and animal studies and human clinical trials have provided corroborating data. Although pectin has many diverse functional qualities, this review focuses on various modifications used to develop MP and its benefits for cancer prevention, bioavailability, clinical trials, and toxicity studies. This review concludes that pectin has anti-cancer characteristics that have been found to inhibit tumor development and proliferation in a wide variety of cancer cells. Nevertheless, further clinical and basic research is required to confirm the chemopreventive or therapeutic role of specific dietary carbohydrate molecules.


Assuntos
Malus , Neoplasias , Animais , Humanos , Pectinas/farmacologia , Pectinas/uso terapêutico , Frutas , Neoplasias/prevenção & controle , Carboidratos da Dieta
10.
Environ Health Perspect ; 130(11): 117003, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36331819

RESUMO

BACKGROUND: Perfluorooctane sulfonate (PFOS) is a persistent environmental pollutant that has become a significant concern around the world. Exposure to PFOS may alter gut microbiota and liver metabolic homeostasis in mammals, thereby increasing the risk of cardiometabolic diseases. Diets high in soluble fibers can ameliorate metabolic disease risks. OBJECTIVES: We aimed to test the hypothesis that soluble fibers (inulin or pectin) could modulate the adverse metabolic effects of PFOS by affecting microbe-liver metabolism and interactions. METHODS: Male C57BL/6J mice were fed an isocaloric diet containing different fibers: a) inulin (soluble), b) pectin (soluble), or c) cellulose (control, insoluble). The mice were exposed to PFOS in drinking water (3µg/g per day) for 7 wk. Multi-omics was used to analyze mouse liver and cecum contents. RESULTS: In PFOS-exposed mice, the number of differentially expressed genes associated with atherogenesis and hepatic hyperlipidemia were lower in those that were fed soluble fiber than those fed insoluble fiber. Shotgun metagenomics showed that inulin and pectin protected against differences in microbiome community in PFOS-exposed vs. control mice. It was found that the plasma PFOS levels were lower in inulin-fed mice, and there was a trend of lower liver accumulation of PFOS in soluble fiber-fed mice compared with the control group. Soluble fiber intake ameliorated the effects of PFOS on host hepatic metabolism gene expression and cecal content microbiome structure. DISCUSSIONS: Results from metabolomic, lipidomic, and transcriptomic studies suggest that inulin- and pectin-fed mice were less susceptible to PFOS-induced liver metabolic disturbance, hepatic lipid accumulation, and transcriptional changes compared with control diet-fed mice. Our study advances the understanding of interaction between microbes and host under the influences of environmental pollutants and nutrients. The results provide new insights into the microbe-liver metabolic network and the protection against environmental pollutant-induced metabolic diseases by high-fiber diets. https://doi.org/10.1289/EHP11360.


Assuntos
Poluentes Ambientais , Inulina , Camundongos , Masculino , Animais , Inulina/metabolismo , Inulina/farmacologia , Lipidômica , Metagenômica , Transcriptoma , Camundongos Endogâmicos C57BL , Fígado , Poluentes Ambientais/metabolismo , Fibras na Dieta/metabolismo , Fibras na Dieta/farmacologia , Pectinas/metabolismo , Pectinas/farmacologia , Mamíferos
11.
Int J Biol Macromol ; 222(Pt A): 521-532, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36184984

RESUMO

Mango is a climacteric fruit and is prone to high perishability. The rapid softening and ripening (due to degradation and disassembly of cell wall polysaccharides) are the major limitations in extending the storability of the harvested mango fruits. Various types of gum-based edible coatings have been reported for the shelf life extension of mango fruits. Tragacanth gum (TCG) also has appropriate coating properties. Its use as an edible coating has been reported on certain fruits. However, the effect of TCG coating in the regulation of harvested mango fruits ripening and softening has not been reported yet. So, the objective of this work was to investigate the effect of TCG (control, 0.5 %, 1 % and 1.5 %) coating on postharvest softening and ripening of harvested mango fruits. TCG coating affected the ripening and softening of mango in a dose-dependent manner. Results exhibited that mango fruits coated with 1.5 % TCG showed substantially lower disease incidence and weight loss. The 1.5 % TCG-coated mangoes showed substantially lower ethylene biosynthesis and respiration rate peaks as well as superoxide anion and hydrogen peroxide contents compared with the control. In the same way, 1.5 % TCG-coated mango fruits had markedly higher total chlorophyll content and lower L*, b* and a* along with substantially lower total carotenoids in peel tissues. Mango fruits coated with 1.5 % TCG exhibited markedly lower water-soluble pectin and higher chelate-soluble pectin, Na2CO3-soluble pectin, protopectin, cellulose and hemicellulose in flesh tissues compared with control. The activity of polygalacturonase (PG), cellulase (CX), pectin methylesterase (PME), ß-galactosidase (ß-Gal) and ß-glucosidase (ß-Glu) were significantly lower in flesh of 1.5 % TCG treated fruits along with substantially higher firmness in contrast with control. In addition, 1.5 % TCG coating treatment showed significantly higher activity of antioxidative enzymes and delayed the increase in soluble solids content (SSC) and ripening index (RI) along with considerably higher titratable acidity (TA) compared with the untreated control. So, pre-storage TCG based edible coating (1.5 %) could be applied to delay ripening and softening in mango fruit industry under postharvest ambient conditions.


Assuntos
Mangifera , Tragacanto , Frutas , Tragacanto/farmacologia , Parede Celular/metabolismo , Polissacarídeos/farmacologia , Pectinas/farmacologia
12.
Food Funct ; 13(22): 11438-11454, 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36314297

RESUMO

The intake of dietary fibers has been associated with a reduction in the risk of colorectal cancer. Pectins - a class of dietary fibers - are polysaccharides that have a complex structure with a wide range of direct and indirect biological beneficial effects on humans. Direct effects include dilution of carcinogens, reduction in cholesterol levels, and interaction with immune cells. Indirect effects include the fermentation and production of short-chain fatty acids. All these biological effects have implications for colon cancer development; however, the exact mechanisms are not fully understood. In this review, we explore the clinical trials regarding dietary fibers and colorectal cancer, thus indicating the potential anti-cancer effects of pectins and modified pectins. We focused on the emerging biological effects of pectins through targeting colorectal cancer hallmark effects and the enabling characteristics. We provide an overview of the mechanisms for each hallmark capability and how the different pectins might exert that anti-cancer effect, such as induction of apoptosis, reduction in cancer cell proliferation and metastasis. The data compilation described herein can guide future clinical trials to investigate how to target specific pectin structures to act as an adjuvant in colon cancer treatment.


Assuntos
Neoplasias do Colo , Pectinas , Humanos , Pectinas/farmacologia , Pectinas/uso terapêutico , Pectinas/química , Fibras na Dieta , Ácidos Graxos Voláteis , Polissacarídeos , Neoplasias do Colo/tratamento farmacológico
13.
Molecules ; 27(19)2022 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-36234870

RESUMO

In this study, keratins were extracted from pig nail waste through the reduction method using L-cysteine as a reductant. Curcumin was successively incorporated in a mixed solution including keratin, gelatin, and glycerin to prepare different kinds of keratin/gelatin/glycerin/curcumin composite films. The morphology of the keratin/ gelatin/glycerin/curcumin composite films were examined using scanning electron microscopy. The structures and the molecular interactions between curcumin, keratin, and pectin were examined using Fourier transform infrared spectroscopy and X-ray diffraction, and the thermal properties were determined through thermogravimetric analysis. The tensile strengths of keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films are 13.73 and 12.45 MPa, respectively, and their respective elongations at break are 56.7% and 4.6%. In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3. Finally, the composite films have a superior antibacterial effect against Staphylococcus aureus and Escherichia coli because of the addition of curcumin. As the concentration of curcumin reaches 1.0%, the antibacterial activity effect of the film is significantly improved. The diameter of the inhibition zone of E. coli is (12.16 ± 0.53) mm, and that of S. aureus is (14.532 ± 0.97) mm. The multifunctional keratin/gelatin/glycerin/curcumin bioactive films have great potential application in the food packaging industry.


Assuntos
Curcumina , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Curcumina/química , Curcumina/farmacologia , Cisteína/farmacologia , Escherichia coli , Embalagem de Alimentos , Gelatina/química , Gelatina/farmacologia , Glicerol/farmacologia , Queratinas/química , Pectinas/farmacologia , Substâncias Redutoras/farmacologia , Espectroscopia de Infravermelho com Transformada de Fourier , Staphylococcus aureus , Suínos
14.
Comput Math Methods Med ; 2022: 1221190, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36267315

RESUMO

The present study was designed to observe the effect of quadruple therapy combined with probiotics on Helicobacter pylori-related peptic ulcer. The patients in the control group (n = 90) were given regular quadruple therapy including proton pump inhibitor ilaprazole enteric-coated tablet + two antibiotics amoxicillin dispersible tablet and metronidazole tablet + colloidal bismuth pectin capsule for 2 weeks. Patients in the study group (n = 90) were given abovementioned quadruple therapy combined with probiotics live combined Bifidobacterium, Lactobacillus, and Enterococcus Capsules, oral for 2 weeks. Then Hp clearance rate, recurrence rate, levels of gastrointestinal hormone makers, and advance reactions between two groups were compared. At the 2nd week after the treatment, the Helicobacter pylori clearance rate in the study group (87.79%) was significantly higher than the control group (78.89%), and the total recurrence rate in the study group (6.67%) was significantly lower than the control group (13.33%) (P < 0.05). Serum gastrin and motilin expression were lower, and somatostatin expressions was significantly higher than those in the control group (P < 0.05). There was no significant difference in the total incidence of adverse reactions between the two groups (P > 0.05). In summary, quadruple therapy combined with probiotics in the treatment of Helicobacter pylori-related peptic ulcer can improve the Helicobacter pylori clearance rate, reduce the Helicobacter pylori recurrence rate, and is beneficial to improving the level of gastrointestinal hormones, with certain safety.


Assuntos
Infecções por Helicobacter , Helicobacter pylori , Úlcera Péptica , Probióticos , Humanos , Infecções por Helicobacter/tratamento farmacológico , Bismuto/farmacologia , Bismuto/uso terapêutico , Metronidazol/farmacologia , Metronidazol/uso terapêutico , Inibidores da Bomba de Prótons/farmacologia , Gastrinas/farmacologia , Gastrinas/uso terapêutico , Motilina/farmacologia , Motilina/uso terapêutico , Comprimidos com Revestimento Entérico/farmacologia , Comprimidos com Revestimento Entérico/uso terapêutico , Quimioterapia Combinada , Úlcera Péptica/tratamento farmacológico , Úlcera Péptica/microbiologia , Amoxicilina/uso terapêutico , Amoxicilina/farmacologia , Antibacterianos/uso terapêutico , Probióticos/uso terapêutico , Pectinas/farmacologia , Pectinas/uso terapêutico , Somatostatina/farmacologia , Somatostatina/uso terapêutico
15.
Molecules ; 27(18)2022 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-36144733

RESUMO

BACKGROUND: Various potential effect of drugs on alleviating diseases by regulating intestinal microbiome as well as the pharmaceutical excipients on gut microbiota has been revealed. However, the interaction between them is rarely investigated. METHODS: Histological analysis, immunohistochemistry analysis, enzyme-linked immunosorbent assay (ELISA) analysis, RT-qPCR, and 16S rRNA analysis were utilized to explore the effect mechanism of the five excipients including hydroxypropyl methylcellulose (HPMC) F4M, Eudragit (EU) S100, chitosan (CT), pectin (PT), and rheum officinale polysaccharide (DHP) on berberine (BBR) to cure UC. RESULTS: The combined BBR with PT and DHP group exhibited better therapeutic efficacy of UC with significantly increased colon length, and decreased hematoxylin-eosin (H&E) scores than other groups. Furthermore, the expression of tight junction ZO-1 and occludin in colon tissue were upregulated, and claudin-2 was downregulated. Ultimately, the serum content of tumor necrosis (TNF)-α, interleukin (IL)-1ß, and IL-6 was decreased. Moreover, the combined BBR with PT significantly promoted the restoration of gut microbiota. The relative abundance of Firmicutes and Lactobacillus was significantly increased by the supplement of PT and DHP, and the relative abundance of Proteobacteria was downregulated. CONCLUSIONS: Our study may provide a new perspective that the selection of pharmaceutical excipients could be a crucial factor affecting the drugs' therapeutic efficiency outcome.


Assuntos
Berberina , Quitosana , Colite Ulcerativa , Colite , Microbioma Gastrointestinal , Animais , Berberina/metabolismo , Quitosana/farmacologia , Claudina-2/metabolismo , Colite/patologia , Colite Ulcerativa/induzido quimicamente , Colite Ulcerativa/tratamento farmacológico , Colo/metabolismo , Sulfato de Dextrana/farmacologia , Modelos Animais de Doenças , Amarelo de Eosina-(YS) , Excipientes/farmacologia , Hematoxilina/metabolismo , Hematoxilina/farmacologia , Hematoxilina/uso terapêutico , Humanos , Derivados da Hipromelose/metabolismo , Interleucina-6/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Ocludina/metabolismo , Pectinas/farmacologia , RNA Ribossômico 16S/genética , RNA Ribossômico 16S/metabolismo
16.
Nutrients ; 14(18)2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36145086

RESUMO

Ulcerative colitis (UC) patients often avoid foods containing fermentable fibers as some can promote symptoms during active disease. Pectin has been identified as a more protective fermentable fiber, but little has been done to determine the interaction between pectin and bioactive compounds present in foods containing that fiber type. Quercetin and chlorogenic acid, two bioactives in stone fruits, may have anti-cancer, anti-oxidant, and anti-inflammatory properties. We hypothesized that quercetin and chlorogenic acid, in the presence of the fermentable fiber pectin, may suppress the expression of pro-inflammatory molecules, alter the luminal environment, and alter colonocyte proliferation, thereby protecting against recurring bouts of UC. Rats (n = 63) received one of three purified diets (control, 0.45% quercetin, 0.05% chlorogenic acid) containing 6% pectin for 3 weeks before exposure to dextran sodium sulfate (DSS, 3% for 48 h, 3x, 2 wk separation, n = 11/diet) in drinking water to initiate UC, or control (no DSS, n = 10/diet) treatments prior to termination at 9 weeks. DSS increased the fecal moisture content (p < 0.05) and SCFA concentrations (acetate, p < 0.05; butyrate, p < 0.05). Quercetin and chlorogenic acid diets maintained SLC5A8 (SCFA transporter) mRNA levels in DSS-treated rats at levels similar to those not exposed to DSS. DSS increased injury (p < 0.0001) and inflammation (p < 0.01) scores, with no differences noted due to diet. Compared to the control diet, chlorogenic acid decreased NF-κB activity in DSS-treated rats (p < 0.05). Quercetin and chlorogenic acid may contribute to the healthy regulation of NF-κB activation (via mRNA expression of IκΒα, Tollip, and IL-1). Quercetin enhanced injury-repair molecule FGF-2 expression (p < 0.01), but neither diet nor DSS treatment altered proliferation. Although quercetin and chlorogenic acid did not protect against overt indicators of injury and inflammation, or fecal SCFA concentrations, compared to the control diet, their influence on the expression of injury repair molecules, pro-inflammatory cytokines, SCFA transport proteins, and NF-κB inhibitory molecules suggests beneficial influences on major pathways involved in DSS-induced UC. Therefore, in healthy individuals or during periods of remission, quercetin and chlorogenic acid may promote a healthier colon, and may suppress some of the signaling involved in inflammation promotion during active disease.


Assuntos
Colite Ulcerativa , Colite , Água Potável , Animais , Anti-Inflamatórios/uso terapêutico , Antioxidantes/metabolismo , Butiratos/metabolismo , Proteínas de Transporte/metabolismo , Ácido Clorogênico/metabolismo , Colite/induzido quimicamente , Colite Ulcerativa/induzido quimicamente , Colite Ulcerativa/tratamento farmacológico , Colite Ulcerativa/prevenção & controle , Colo/metabolismo , Citocinas/metabolismo , Sulfato de Dextrana , Dieta , Fibras na Dieta/metabolismo , Modelos Animais de Doenças , Água Potável/metabolismo , Fator 2 de Crescimento de Fibroblastos/metabolismo , Inflamação/metabolismo , Interleucina-1/metabolismo , Peptídeos e Proteínas de Sinalização Intracelular , NF-kappa B/genética , NF-kappa B/metabolismo , Pectinas/metabolismo , Pectinas/farmacologia , Quercetina/metabolismo , Quercetina/farmacologia , RNA Mensageiro/metabolismo , Ratos
17.
J Mater Chem B ; 10(40): 8218-8234, 2022 10 19.
Artigo em Inglês | MEDLINE | ID: mdl-36173240

RESUMO

Clinically frequently-used glutaraldehyde (GA)-crosslinked bioprosthetic valve leaflets (BVLs) are still curbed by acute thrombosis, malignant immunoreaction, calcification, and poor durability. In this study, an anticoagulant heparin-like biomacromolecule, sulfonated, oxidized pectin (SAP) with a dialdehyde structure was first obtained by modifying citrus pectin with sulfonation of 3-amino-1-propane sulfonic acid and then oxidating with periodate. Notably, a novel crosslinking approach was established by doubly crosslinking BVLs with SAP and the nature-derived crosslinking agent quercetin (Que), which play a synergistic role in both crosslinking and bioactivity. The double crosslinked BVLs also presented enhanced mechanical properties and enzymatic degradation resistance owing to the double crosslinking networks formed via CN bonds and hydrogen bonds, respectively, and good HUVEC-cytocompatibility. The in vitro and ex vivo assay manifested that the double-crosslinked BVLs had excellent anticoagulant and antithrombotic properties, owing to the introduction of SAP. The subcutaneous implantation also demonstrated that the obtained BVLs showed a reduced inflammatory response and great resistance to calcification, which is attributed to quercetin with multiple physiological activities and depletion of aldehyde groups by hydroxyl aldehyde reaction. With excellent stability, hemocompatibility, anti-inflammatory, anti-calcification, and pro-endothelialization properties, the obtained double-crosslinked BVLs, SAP + Que-PP, would have great potential to substitute the current clinical GA-crosslinked BVLs.


Assuntos
Bioprótese , Calcinose , Próteses Valvulares Cardíacas , Humanos , Glutaral/química , Quercetina/farmacologia , Propano , Fibrinolíticos , Reagentes de Ligações Cruzadas/química , Calcinose/patologia , Pectinas/farmacologia , Heparina , Anticoagulantes/farmacologia , Ácidos Sulfônicos
18.
Int J Biol Macromol ; 222(Pt A): 671-679, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36174858

RESUMO

Factors causing differences in immune activities between pectin and pectin-derived oligosaccharides have not been fully studied. In this article, four samples with different molecular weights and monosaccharide compositions, including polygalacturonic acid (poly-GA) and its oligosaccharide (oligo-GA), navel orange peel pectin (NP) and its oligosaccharide (oligo-NP), were used to compare their immunomodulatory properties on RAW264.7 cells. All samples had nontoxic effect on cells, oligo-GA and oligo-NP could increase the production of nitric oxide and cytokines to a much higher level than poly-GA and NP. The findings revealed that reducing the molecular weight and preserving the branched regions of pectin-derived samples could improve their immune-enhancing effects on macrophages. Interestingly, the addition of TAK-242 (TLR4 inhibitor) also demonstrated that the tested pectin oligosaccharides could stimulate the activation of macrophages through TLR4 signaling pathway. These results confirmed the potential value of pectin oligosaccharides, and provided theoretical support for their application in the pharmaceutical industry.


Assuntos
Pectinas , Receptor 4 Toll-Like , Pectinas/farmacologia , Pectinas/metabolismo , Oligossacarídeos/farmacologia , Monossacarídeos , Peso Molecular
19.
Int J Biol Macromol ; 222(Pt A): 701-714, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36170930

RESUMO

Wound healing is a complicated cellular process with overlapping phases. Naringin (NAR); a flavanone glycoside, possesses numerous pharmacological effects such as anti-inflammatory, antioxidant and anti-apoptotic effects. In the current study, Arabic gum (AG)/pectin hydrogel was utilized to encapsulate NAR. Drug-loaded AG/pectin hydrogel exhibited excellent EE% of about 99.88 ± 0.096 and high DL% of about 16.64 ± 0.013. The formulated drug-loaded hydrogel was characterized using Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), scanning electron microscopy (SEM) and Zetasizer analyzer, besides determination of equilibrium degree of swelling (EDS%). Afterwards, wound healing potential of NAR-loaded AG/pectin hydrogel was evaluated in an in vivo animal model. Results manifested that NAR-loaded AG/pectin hydrogel was able to accelerate wound healing in terms of enhanced angiogenesis, re-epithelialization and collagen deposition. Furthermore, it significantly (P < 0.001) down-regulated the mRNA expression of inflammatory mediators (TNF-α) and apoptosis (BAX). In addition, NAR-loaded AG/pectin hydrogel was found to possess potent antioxidant activity as it enhanced the levels of SOD and GSH, besides decreasing the levels of MPO, MDA and nitrite. These data suggest that NAR-loaded AG/pectin hydrogel could be utilized in wound healing applications.


Assuntos
Flavanonas , Hidrogéis , Animais , Hidrogéis/química , Pectinas/farmacologia , Cicatrização , Flavanonas/farmacologia
20.
J Food Biochem ; 46(9): e14380, 2022 09.
Artigo em Inglês | MEDLINE | ID: mdl-35976799

RESUMO

In this paper, a pectin polysaccharide AP2-c with molecular weight 6.69 × 105 Da was obtained from the lignified okra. The monosaccharide composition analysis indicated that AP2-c consisted of galactose, rhamnose and galacturonic acid in a molar ratio of 2.3: 1.5: 1.5. The structural characterization indicated that the main chain of AP2-c was composed of →2)-α-L-Rhap-(1→ and →4)-α-D-GalAp-(1→. →2)-α-L-Rhap-(1→ was branched at position O-4 and the branched chain consisted of →3,6)-ß-D-Galp-(1→, →6)-ß-D-Galp-(1→, α-L-Rhap-(1→ and ß-D-Galp-(1→. AP2-c could inhibit the mRNA expression levels of TNF-α, IL-1ß and iNOS in LPS-induced macrophages with a dose-dependent manner. Furthermore, AP2-c inhibited the phosphorylation of IκB and p65 via NF-κB pathway. The results indicated that AP2-c had obvious anti-inflammatory activity. PRACTICAL APPLICATIONS: When okra seeds were harvested, lignified okra was always abandoned as waste and had not been fully used for exploitation. Nevertheless, it accounted for more than half of the total plant's weight and was abundant in cell wall polysaccharides, which were the main components of okra to perform a variety of biological functions. In the research, the purified pectin polysaccharide AP2-c was obtained from lignified okra and its physicochemical properties, structural features and anti-inflammatory activity were systematically researched. It was detected that AP2-c exhibited anti-inflammatory activity by blocking NF-κB pathway and thus lowering the expression of related inflammatory factors. The results have significant implications for the value-added application of okra and its processing side products can obviously help to promote the anti-inflammatory application of AP2-c and avoid wasting resources.


Assuntos
Abelmoschus , Abelmoschus/química , Abelmoschus/metabolismo , Anti-Inflamatórios/farmacologia , NF-kappa B/genética , NF-kappa B/metabolismo , Pectinas/química , Pectinas/farmacologia , Polissacarídeos/química , Polissacarídeos/farmacologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...