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1.
J Parasitol ; 106(1): 56-70, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31995719

RESUMO

Rhadinorhynchus hiansi Soota and Bhattacharya, 1981 , has remained unknown since its original incomplete description from 2 male specimens collected from the flat needlefish Ablennes hians Valenciennes (Belonidae) off Trivandrum, Kerala, India. Recent collections of fishes along the Pacific coast of Vietnam in 2016 and 2017 produced many specimens of the same species from the striped bonito Sarda orientalis Temminck and Schlegel (Scombridae) off the southern Pacific coast of Vietnam at Nha Trang. We describe females for the first time, assign a female allotype status, and provide an expanded description of males from a larger collection completing missing information on hooks and hook roots, receptacle, lemnisci, cement glands, Saefftigen's pouch, and trunk spines. Specimens of R. hiansi characteristically have no dorsal spines in the posterior field of trunk spines and a long proboscis with 36-48 dorso-ventrally differentiated proboscis hooks per row becoming progressively smaller posteriorly then increasing in size near the posterior end to a maximum at the posterior-most ring. Trunk, testes, and lemnisci in our specimens were considerably larger than those reported in the original description, but the proboscis was relatively smaller. The females had long reproductive system and corrugated elliptic eggs without polar prolongation of fertilization membrane. Energy Dispersive X-ray Analysis (EDXA) demonstrates high levels of calcium and phosphorus in large gallium cut hooks and high levels of sulfur in tip cuts of large and small hooks and in spines. This EDXA pattern is a characteristic fingerprint of R. hiansi. The molecular profile of R. hiansi is described from 18S rDNA and COI genes, and phylogenetic relationships with most closely related species are discussed.


Assuntos
Acantocéfalos/anatomia & histologia , Acantocéfalos/genética , Beloniformes/parasitologia , Doenças dos Peixes/parasitologia , Helmintíase Animal/parasitologia , Acantocéfalos/classificação , Acantocéfalos/ultraestrutura , Algoritmos , Animais , Teorema de Bayes , Feminino , Peixes , Gálio , Funções Verossimilhança , Masculino , Microscopia Eletrônica de Varredura/veterinária , Oceano Pacífico , Filogenia , Espectrometria por Raios X/veterinária , Vietnã
2.
Ying Yong Sheng Tai Xue Bao ; 31(1): 293-300, 2020 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-31957407

RESUMO

It is important to reveal the interspecific association and correlation of main species for deep understanding of fish community structure and function. Based on data collected from the bottom trawl surveys from 2013 to 2017 in the Haizhou Bay and the adjacent waters, we analyzed interspecific association of main species using Chi-square test, Jaccard index, and Spearman rank correlation coefficient. There were consistent results from χ2-test, Jaccard index, and Spearman's rank correlation analysis. The proportion of positively correlated species pairs was high in different years, whereas the number of significant or extremely significant species pairs was low. There were 9, 6, 7, 1, 0 significant or extremely significant species pairs over different years based on Jaccard index. There were 12, 20, 13, 5, 6 significant or extremely significant species pairs over different years based on Spearman's rank correlation coefficient. While there were inter-annual changes in related species pairs, the relationship between significant species pairs was relatively stable, indicating a relatively stable interspecific association in the main fish communities. The species within the same ecological type could replace each other in the niche, which have adapted to environmental changes in Haizhou Bay and its adjacent waters.


Assuntos
Baías , Peixes , Animais , Ecologia , Estações do Ano
3.
Ying Yong Sheng Tai Xue Bao ; 31(1): 301-308, 2020 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-31957408

RESUMO

We measured the ratio of δ13C and δ15N values to estimate the trophic level of main organisms based on a fisheries resource survey in coastal water of Lyusi fishing ground carried out in September 2018. The results showed that δ13C values of the main organisms ranged from -24.27‰ to -13.24‰, with an average of (-17.15±1.85)‰. The δ15N values ranged from 4.30‰ to 14.61‰, with an average of (11.21±1.90)‰. Results from cluster analysis demonstrated that the main organisms in the coastal water of Lyusi fishery ground could be divided into four trophic groups. The first group was consisted mainly by middle and small fishes, shrimps and other invertebrates. The second group included the small-sized fishes like Konosirus punctatus, Mugil cephalus, etc. and shrimps like Exopalaemon annandalei, Exopalamon carincauda, etc. The phytoplankton belonged to the third group. The fourth group was zooplankton. In the trophic spectrum, the trophic levels of fish, shrimps and crabs, shellfish were 3.2-4.7, 3.2-4.2 and 2.0-4.1, respectively. Most species belonged to the category of middle and high-level consumers. In this survey, the average trophic level of the same species in the coastal waters of Lyusi fishing ground was 0.6, which was higher than that in the East China Sea and Yellow Sea. There was niche overlap of most fish, shrimps and crabs. Those results indicated the growth environment and nutrient structure of most living organisms in this area were generally similar.


Assuntos
Cadeia Alimentar , Zooplâncton , Animais , Isótopos de Carbono , China , Peixes , Isótopos de Nitrogênio
4.
Dis Aquat Organ ; 137(3): 195-204, 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31942865

RESUMO

Golden pompano Trachinotus ovatus (Linnaeus, 1758) is an important mariculture fish species with high commercial value in China. The present study thoroughly assessed the types and frequencies of skeletal deformities at the early developmental stages of golden pompano in an intensive aquaculture production system. Golden pompano (n = 500) were sampled 30 d posthatch (dph). The specimens were stained with Alcian blue and Alizarin red for the detection of deformities. The results of the study revealed that 77.2% of the specimens showed at least 1 spinal anomaly; most anomalies occurred in the prehemal region, and the most common deformity observed was vertebral fusion (37.4% incidence of deformities). The results of this study provide useful information for the early detection of skeletal deformities and for the optimization of fish fry breeding technologies.


Assuntos
Doenças dos Peixes , Perciformes , Animais , Aquicultura , China , Peixes
5.
Mar Genomics ; 49: 100717, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31680057

RESUMO

The blood of fish has a continuous age distribution of erythrocytes. The properties of young and old erythrocytes differ with young erythrocytes being functionally much more versatile than old erythrocytes, which have higher haemoglobin content. Factors which affect the formation and breakdown of erythrocytes are reviewed. Erythropoiesis in fish is largely similar to that in mammals. However, definitive erythrocytes are mainly formed in the anterior part of the kidney, and erythropoietin is secreted mainly from the heart. Senescence of erythrocytes in fish has not been studied in detail, and consecutively the factors causing aging of erythrocytes in man are discussed. A major factor causing aging of erythrocytes is oxidative stress, which is also a major effect of toxicants on fish erythrocytes. Consequently, apparent age distribution of circulating erythrocytes will be affected by environmental pollution. At present, it is completely unknown, if the changes in age distribution of erythrocytes play a role in pollutant responses or if the age distribution of erythrocytes is actively regulated during environmental adaptation.


Assuntos
Senescência Celular , Eritrócitos/citologia , Peixes , Animais , Meio Ambiente , Eritropoese , Estresse Oxidativo
6.
Mar Genomics ; 49: 100724, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31735579

RESUMO

The ancient origins and functional versatility of globins make them ideal subjects for studying physiological adaptation to environmental change. Our goals in this review are to describe the evolution of the vertebrate globin gene superfamily and to explore the structure/function relationships of hemoglobin, myoglobin, neuroglobin and cytoglobin in teleost fishes. We focus on the globins of Antarctic notothenioids, emphasizing their adaptive features as inferred from comparisons with human proteins. We dedicate this review to Guido di Prisco, our co-author, colleague, friend, and husband of C.V. Ever thoughtful, creative, and enthusiastic, Guido spearheaded study of the structure, function, and evolution of the hemoglobins of polar fishes - this review is testimony to his wide-ranging contributions. Throughout his career, Guido inspired younger scientists to embrace polar biological research, and he challenged researchers of all ages to explore evolutionary adaptation in the context of global climate change. Beyond his scientific contributions, we will miss his warmth, his culture, and his great intellect. Guido has left an outstanding legacy, one that will continue to inspire us and our research.


Assuntos
Adaptação Fisiológica , Evolução Molecular , Peixes/genética , Globinas/genética , Sequência de Aminoácidos , Animais , Regiões Antárticas , Citoglobina/genética , Hemoglobinas/genética , Família Multigênica , Mioglobina/genética , Neuroglobina/genética , Sintenia
7.
J Sci Food Agric ; 100(3): 1285-1293, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31710695

RESUMO

BACKGROUND: Bioprocessing of plant feedstuff can be a novel approach for reducing the overwhelming dependence on fish meal in aquaculture. The objective of this study was to evaluate the performance of Pacific white shrimp Litopenaeus vannamei fed solid-state fermented protein concentrates in order to replace fish meal in the diet. RESULTS: In the first trial, a group of 15 shrimp (average 3.88 g) were randomly distributed into aquaria in triplicate according to the experimental diets. Ten isonitrogenous (400 g kg-1 CP) and isolipidic (90 g kg-1 CL) diets were formulated to contain high-protein fish meal (HFM) and low-protein fish meal (LFM), and four types of bioprocessed protein concentrates (BPCs) as a replacement of fish meal (BPC-A, -B, -C and -D) each at 30% and 50% FM replacement levels. BPC-A was a solid-state fermented mixture of soybean and corn gluten meals; BPC-B was pre-treated acid-hydrolyzed BPC-A; BPC-C and BPC-D were BPC-A + 2% shrimp soluble extract (SSE) and BPC-B + 2% SSE, respectively. After 8 weeks, shrimp fed the HFM, BPC-B, BPC-C and BPC-D diets showed significantly higher growth performance at 30% FM replacement than those of shrimp fed the BPC diets at 50% FM replacement. Interestingly, shrimp fed the BPC-D diet could replace up to 50% FM replacement. In the second trial, the results show that apparent digestibility coefficients of feeds and apparent digestibility coefficients of ingredients for crude protein were significantly higher in fish fed the BPC-B, BPC-C and BPC-D diets. CONCLUSIONS: The results demonstrated successful partial replacement of high-protein fish meal using high-quality fermented protein concentrates from plant sources. © 2019 Society of Chemical Industry.


Assuntos
Ração Animal/análise , Proteínas de Peixes/metabolismo , Penaeidae/crescimento & desenvolvimento , Penaeidae/metabolismo , Proteínas de Plantas/metabolismo , Soja/metabolismo , Animais , Aquicultura , Dieta/veterinária , Digestão , Proteínas de Peixes/análise , Peixes/metabolismo , Proteínas de Plantas/análise , Soja/química
8.
Mar Genomics ; 49: 100721, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31711848

RESUMO

Since the discovery of new members of the globin superfamily such as Cytoglobin, Neuroglobin and Globin X, in addition to the most well-known members, Hemoglobin and Myoglobin, different hypotheses have been suggested about their function in vertebrates. Globins are ubiquitously found in living organisms and can carry out different functions based on their ability to bind ligands such as O2, and nitric oxide (NO) and to catalyze reactions scavenging NO or generating NO by reducing nitrite. NO is a highly diffusible molecule with a central role in signaling important for egg maturation, fertilization and early embryonic development. The globins ability to scavenge or generate NO makes these proteins ideal candidates in regulating NO homeostasis depending on the micro environment and tissue NO demands. Different amounts of various globins have been found in zebrafish eggs and developing embryos where it's unlikely that they function as respiratory proteins and instead could play a role in maintaining embryonic NO homeostasis. Here we summarize the current knowledge concerning the role of NO in adult fish in comparison to mammals and we discuss NO function during embryonic development with possible implications for globins in maintaining embryonic NO homeostasis.


Assuntos
Desenvolvimento Embrionário , Peixes/embriologia , Globinas/fisiologia , Óxido Nítrico/fisiologia , Animais , Homeostase
9.
Oecologia ; 192(1): 143-154, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31709458

RESUMO

Functional traits can be used to identify the importance of various community assembly mechanisms such as ecological drift, environmental filtering, and limiting similarity. These processes act in concert, not isolation, and different processes may act upon separate traits, potentially concealing the ecological signal of one or more of the mechanisms. Nine functional attributes of marine fish were used to identify changes in the importance of various mechanisms in the assembly of marine fish communities over a latitudinal gradient along the Western Australian coast. Complementary null modelling approaches were used to test the relative importance of assembly processes (ecological drift, environmental filtering, and limiting similarity) in structuring fish communities. Ecological drift was found to be a major driver of the structure of fish communities, and dispersal limitation was strongest in the tropical region, with homogenising dispersal strongest in the temperate region. Dispersion of functional traits identified environmental filtering acting on most traits incorporated in this study, in addition to limiting similarity acting on traits associated with acquisition of trophic resources. The coexistence of Western Australian marine fishes thus results from concurrent ecological drift, environmental filtering, and limiting similarity structuring the communities. The observed ecological drift may be the result of priority effects and/or context-dependent biotic interactions. Both niche complementarity and predator avoidance may be the drivers of the observed limiting similarity in the communities.


Assuntos
Ecossistema , Peixes , Animais , Austrália , Ecologia , Fenótipo
10.
J Agric Food Chem ; 68(1): 418-425, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31829625

RESUMO

For quick, noninvasive, and high-sensitivity surface analysis of foods and agricultural products, a touch sensor was developed and applied to sheath-flow probe electrospray ionization/mass spectrometry (sfPESI/MS). Upon making contact with the sample, the probe stopped by detecting the current flowing through the circuit and analytes on the sample surface were extracted in the solvent preloaded in the plastic capillary. By lifting up the probe to the default position, an electrospray ionization mass spectrum of the sample was obtained. By scanning the sample stage using a programming tool, a point analysis of targeted positions of biological samples with a spot diameter of ≤0.3 mm was achieved. It took less than 10 s for one sample spot. This method was applied to various plants and animal tissues.


Assuntos
Análise de Alimentos/métodos , Espectrometria de Massas por Ionização por Electrospray/métodos , Animais , Galinhas , Decapodiformes/química , Peixes , Análise de Alimentos/instrumentação , Frutas/química , Carne/análise , Plantas/química , Espectrometria de Massas por Ionização por Electrospray/instrumentação
11.
Bioresour Technol ; 297: 122542, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31830720

RESUMO

The effect of different mixing ratios of fish processing waste (FPW) with a liquid fraction (LF) of hydrothermal carbonization (HTC) of bamboo residues on biogas and methane yield was investigated. The different mixing ratios (FPW + LF) and HTC temperature (200-280 °C) had significant effects on biogas and methane production. The anaerobic co-digestion of the various mixing ratio of FPW and LF of bamboo residues did not enhance the methane yield compared to the AD of FPW alone. However, a mixture of 75FPW + 25LF(2 2 0) presented a comparable methane production (133 mL/g VS) to that achieved with 100FPW (142 mL/g VS), which represents an increase of only 6.4%. The ratio of 75FPW + 25LF(2 2 0) increased the biogas yield by 81% compared to the control group of 100LF(2 2 0). The mixing ratio of 75 FPW + 25LF(2 2 0) did not require clean water input to dilute FPW for biogas production and can be a practical waste management method.


Assuntos
Biocombustíveis , Metano , Anaerobiose , Animais , Reatores Biológicos , Peixes , Alimentos Marinhos
12.
Ambio ; 49(2): 531-540, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31140158

RESUMO

Artificial water bodies like ditches, fish ponds, weirs, reservoirs, fish ladders, and irrigation channels are usually constructed and managed to optimize their intended purposes. However, human-made aquatic systems also have unintended consequences on ecosystem services and biogeochemical cycles. Knowledge about their functioning and possible additional ecosystem services is poor, especially compared to natural ecosystems. A GIS analysis indicates that currently only ~ 10% of European surface waters are covered by the European Water Framework directive, and that a considerable fraction of the excluded systems are likely human-made aquatic systems. There is a clear mismatch between the high possible significance of human-made water bodies and their low representation in scientific research and policy. We propose a research agenda to build an inventory of human-made aquatic ecosystems, support and advance research to further our understanding of the role of these systems in local and global biogeochemical cycles as well as to identify other benefits for society. We stress the need for studies that aim to optimize management of human-made aquatic systems considering all their functions and to support programs designed to overcome barriers of the adoption of optimized management strategies.


Assuntos
Ecossistema , Peixes , Animais , Humanos
13.
Ambio ; 49(2): 391-406, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31168701

RESUMO

Declining physiological status in marine top consumers has been observed worldwide. We investigate changes in the physiological status and population/community traits of six consumer species/groups in the Baltic Sea (1993-2014), spanning four trophic levels and using metrics currently operational or proposed as indicators of food-web status. We ask whether the physiological status of consumers can be explained by food-web structure and prey food value. This was tested using partial least square regressions with status metrics for gray seal, cod, herring, sprat and the benthic predatory isopod Saduria as response variables, and abundance and food value of their prey, abundance of competitors and predators as predictors. We find evidence that the physiological status of cod, herring and sprat is influenced by competition, predation, and prey availability; herring and sprat status also by prey size. Our study highlights the need for management approaches that account for species interactions across multiple trophic levels.


Assuntos
Cadeia Alimentar , Comportamento Predatório , Animais , Países Bálticos , Peixes , Alimentos Marinhos
14.
Ambio ; 49(2): 640-649, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31201615

RESUMO

Understanding fisher beliefs and attitudes towards specific management strategies can help inform and improve fisheries management, and thus stock sustainability. Previous studies highlight a lack of fisher awareness regarding environmental issues influencing the systems they utilise and the negative impacts of specific strategies, such as stock enhancement. Our study used a two-phase approach to first elicit and then measure the strength of common fishers' beliefs and associated attitudes regarding stock enhancement. Specifically, this research focused on recreational fishers of an estuarine crab fishery (Portunus armatus) in south-western Australia. The results demonstrate that recreational fishers believe stock enhancement could have strong positive outcomes, but also recognise that this management strategy could lead to some negative outcomes, though the latter are perceived as less likely to happen. This contrasts with previous research on fisheries stocking and demonstrates the value of using the two-phase approach to clarify fishers' perceptions of particular management approaches. To reduce fisher dissatisfaction with management actions, careful communication on the benefits and costs of stock enhancement is recommended. Our study highlights the significance of integrating social sciences into fisheries research, and the need to better understand fishing community beliefs to ensure effective management of the fishery.


Assuntos
Conservação dos Recursos Naturais , Pesqueiros , Peixes
15.
Adv Exp Med Biol ; 1131: 281-320, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31646515

RESUMO

In mammalian cardiomyocytes, Ca2+ influx through L-type voltage-gated Ca2+ channels (VGCCs) is amplified by release of Ca2+ via type 2 ryanodine receptors (RyR2) in the sarcoplasmic reticulum (SR): a process termed Ca2+-induced Ca2+-release (CICR). In mammalian skeletal muscles, VGCCs play a distinct role as voltage-sensors, physically interacting with RyR1 channels to initiate Ca2+ release in a mechanism termed depolarisation-induced Ca2+-release (DICR). In the current study, we surveyed the genomes of animals and their close relatives, to explore the evolutionary history of genes encoding three proteins pivotal for ECC: L-type VGCCs; RyRs; and a protein family that anchors intracellular organelles to plasma membranes, namely junctophilins (JPHs). In agreement with earlier studies, we find that non-vertebrate eukaryotes either lack VGCCs, RyRs and JPHs; or contain a single homologue of each protein. Furthermore, the molecular features of these proteins thought to be essential for DICR are only detectable within vertebrates and not in any other taxonomic group. Consistent with earlier physiological and ultrastructural observations, this suggests that CICR is the most basal form of ECC and that DICR is a vertebrate innovation. This development was accompanied by the appearance of multiple homologues of RyRs, VGCCs and junctophilins in vertebrates, thought to have arisen by 'whole genome replication' mechanisms. Subsequent gene duplications and losses have resulted in distinct assemblies of ECC components in different vertebrate clades, with striking examples being the apparent absence of RyR2 from amphibians, and additional duplication events for all three ECC proteins in teleost fish. This is consistent with teleosts possessing the most derived mode of DICR, with their Cav1.1 VGCCs completely lacking in Ca2+ channel activity.


Assuntos
Canais de Cálcio Tipo L , Evolução Molecular , Acoplamento Excitação-Contração , Canal de Liberação de Cálcio do Receptor de Rianodina , Animais , Canais de Cálcio Tipo L/metabolismo , Acoplamento Excitação-Contração/genética , Peixes/genética , Peixes/metabolismo , Genoma/genética , Músculo Esquelético/fisiologia , Miócitos Cardíacos/fisiologia , Canal de Liberação de Cálcio do Receptor de Rianodina/metabolismo , Retículo Sarcoplasmático/fisiologia
16.
Artigo em Inglês | MEDLINE | ID: mdl-31525459

RESUMO

As the first marine teleost demonstrated to biosynthesize long-chain polyunsaturated fatty acids (LC-PUFAs) from C18 precursors such as linoleic acid (LOA, 18:2n-6) and α-linolenic acid (ALA, 18:3n-3), the rabbitfish (Siganus canaliculatus) contains the complete enzymatic system for LC-PUFA biosynthesis, including Δ6/Δ5 fatty acid desaturase (Fad), Δ4 Fad, and elongase 5 (Elovl5). Previously, our group demonstrated that hepatocyte nuclear factor 4α (Hnf4α) is a transcription factor (TF) for rabbitfish Δ4 fad and elovl5, and interacts with the core promoter of Δ6/Δ5 fad. To fully clarify the role of Hnf4α in the regulation of LC-PUFA biosynthesis, the present study aimed to explore the regulatory role of Hnf4α on Δ6/Δ5 fad gene expression. First, Hnf4α overexpression and agonist assays identified the Hnf4α response region in the Δ6/Δ5 fad core promoter as -456 bp to +51 bp. Bioinformatic analysis predicted four potential Hnf4α binding elements in the core promoter, which were confirmed by site-directed mutation and functional assays in a dual luciferase assay system. Moreover, the mRNA expression levels of hnf4α, Δ6/Δ5 fad, and Δ4 fad were significantly increased in the S. canaliculatus hepatocyte line (SCHL) cells after treatment with Hnf4α agonists (Alverine and Benfluorex) or its mRNA overexpression. By contrast, the expression levels of these three genes were markedly decreased after hnf4a small interfering RNA (siRNA) transfection. The results indicated that Hnf4α has a regulatory effect on rabbitfish Δ6/Δ5 fad gene transcription, identifying Hnf4α as a TF of Δ6/Δ5 fad in vertebrates for the first time.


Assuntos
Ácidos Graxos Dessaturases/biossíntese , Proteínas de Peixes/metabolismo , Peixes/metabolismo , Regulação Enzimológica da Expressão Gênica/fisiologia , Fator 4 Nuclear de Hepatócito/metabolismo , Linoleoil-CoA Desaturase/biossíntese , Animais , Ácidos Graxos Dessaturases/genética , Proteínas de Peixes/genética , Peixes/genética , Fator 4 Nuclear de Hepatócito/genética , Linoleoil-CoA Desaturase/genética
17.
Food Chem ; 305: 125508, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31622806

RESUMO

Fish is one of the most common elicitors of food-allergic reactions worldwide. These reactions are triggered by the calcium-binding muscle protein ß-parvalbumin, which was shown to have reduced immunoglobulin E (IgE)-binding capacity upon calcium depletion. This work aimed to reduce gilthead seabream allergenicity using diets supplemented with a calcium chelator. Three experimental feeds were tested, differing in ethylenediaminetetraacetic acid (EDTA) supplementation, and its effects on muscle and parvalbumin's IgE-reactivity were analyzed. Chromatographic determination of EDTA showed no accumulation in the muscle and sensory results demonstrated that the lowest concentration did not affect fish quality as edible fish. Proteomics revealed one protein related to muscle contraction with significantly different relative abundance. Immunoblot assays performed with fish-allergic patients sera indicated a 50% reduction in IgE-reactivity upon EDTA presence. These preliminary results provide the basis for the further development of a non-GMO approach to modulate fish allergenicity and improve safety of aquaculture fish.


Assuntos
Ração Animal/análise , Aquicultura , Ácido Edético/análise , Peixes/imunologia , Qualidade dos Alimentos , Músculos/química , Proteômica , Animais , Peixes/metabolismo , Humanos , Imunoglobulina E/imunologia , Parvalbuminas/imunologia , Alimentos Marinhos
18.
Chemosphere ; 240: 124878, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31563719

RESUMO

Dihydroxylated polybrominated diphenyl ethers (diOH-PBDEs) can be natural products of marine organisms or the metabolites of PBDEs. The optimal determination method and concentration of diOH-PBDEs in seafood are unknown due to a lack of commercially available standards. In the present study, diOH-PBDEs were synthesized, and an efficient measurement method for OH-PBDEs and diOH-PBDEs in sea fish muscle samples, including extraction, clean-up and gas chromatography-tandem mass spectrometry (GC-MS/MS) analysis, was established. Pressurized liquid extraction (PLE) followed by partitioning with a KOH solution and florisil cartridge clean-up proved to be a reliable and robust method for detecting all OH-PBDEs/diOH-PBDEs. GC-MS/MS with an electron ionization (EI) source analysis was a sensitive analytical instrument for OH-PBDEs/diOH-PBDEs. The recovery using this method ranged from 19% to 101%, 28%-88% and 42%-90% for 10 ng, 20 ng and 40 ng spiking levels, respectively. The equipment detection limits (EDLs) were in the range of 0.31-2.78 pg/µL, and the limits of detection (LOD) for the method were in the range of 5.07-38.74 pg/g wet weight. Concentrations of diOH-PBDEs in the marine fish muscle samples were in the range of 32.43-1528.63 pg/g wet weight. Similar compositions of OH-PBDEs/diOH-PBDEs were found within the same family of marine fish.


Assuntos
Peixes/metabolismo , Cromatografia Gasosa-Espectrometria de Massas/métodos , Éteres Difenil Halogenados/análise , Espectrometria de Massas em Tandem/métodos , Animais , Limite de Detecção
19.
Ecotoxicol Environ Saf ; 187: 109833, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31654862

RESUMO

The utilization of fish scale-derived biosorbent for immobilization of cadmium and lead in polluted sediment was thoroughly investigated in this study. Fish scale (FS) biomass was chemically and physically treated. The FS biomass treated with acid (0.1 M HCl), alkali (0.1 M NaOH) and hydrothermally, showed minimum removal capacity. While, FS treated hydrothermally along with acid showed the maximum removal efficiency of metal ions. We used different dosages (0%, 2.5%, 5%, 10%, 15%, and 20%) of FS biosorbent in the sediment. Isotherm modelling showed that this biosorbent can hold 89.30 and 92.65 mg/g of Cd and Pb on its surface. This indicated that prepared FS biosorbent has enough potential to adsorb Cd and Pb ions on its surface from the sediment. Compared to the control, sediment treated with 20% FS showed the highest immobilization capacities for Pb (92.9%), and Cd (87.9%). The values of partition coefficient (Kd) increased by 83% for Pb and 78% for Cd, which specified that availability of free ions of Pb and Cd in the aquatic system was successfully decreased. The sediment treated with 20% FS biosorbent showed 70-80% immobilization of Cd and Pb from mobile and exchangeable fractions that ultimately decreased the bioavailability of metal ions to the biota. Inclusively, compared to control, sediment served with 20% FS biosorbent showed higher level of Pb and Cd ions in residual fraction near by 80%. The prepared FS biosorbent had shown its potential in immobilizing the Cd and Pb ions from sediment as a cheap and ecologically feasible method for amendment.


Assuntos
Escamas de Animais/química , Peixes , Metais Pesados/química , Eliminação de Resíduos/métodos , Poluentes Químicos da Água/química , Adsorção , Animais , Biodegradação Ambiental , Cádmio/química , Chumbo/química
20.
Ambio ; 49(1): 130-143, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30843168

RESUMO

The transformation of coral reefs has profound implications for millions of people. However, the interactive effects of changing reefs and fishing remain poorly resolved. We combine underwater surveys (271 000 fishes), catch data (18 000 fishes), and household surveys (351 households) to evaluate how reef fishes and fishers in Moorea, French Polynesia responded to a landscape-scale loss of coral caused by sequential disturbances (a crown-of-thorns sea star outbreak followed by a category 4 cyclone). Although local communities were aware of the disturbances, less than 20% of households reported altering what fishes they caught or ate. This contrasts with substantial changes in the taxonomic composition in the catch data that mirrored changes in fish communities observed on the reef. Our findings highlight that resource users and scientists may have very different interpretations of what constitutes 'change' in these highly dynamic social-ecological systems, with broad implications for successful co-management of coral reef fisheries.


Assuntos
Antozoários , Recifes de Corais , Animais , Ecossistema , Pesqueiros , Peixes , Ilhas do Pacífico
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