Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.752
Filtrar
1.
Ulus Travma Acil Cerrahi Derg ; 25(4): 350-354, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31297773

RESUMO

BACKGROUND: Chest injuries, accounting for 25% of all trauma-related deaths, are one of the main causes of death in young adults. Our priority is the early identification of life-threatening injuries both immediate and delayed. The role of various biomarkers, such as Clara cell protein 16, von Willebrand factor, interleukin-6, tumor necrosis factor, and angiopoietin, has been studied in trauma-related acute respiratory distress syndrome (ARDS). Serum angiotensin-converting enzyme (ACE) levels have been studied in non-trauma-related ARDS. The aim of this prospective observational study was to evaluate the role of ACE levels as a prognostic marker in thoracic trauma. METHODS: A prospective observational study was conducted to evaluate serum ACE levels in thoracic trauma patients and to explore its prognostic potential with regard to clinical outcome. A total of 48 thoracic trauma patients were included in the study. RESULTS: The mean ACE level in the study population was 66.54+-11.18. A strong positive correlation was found among serum ACE levels and Thoracic Trauma Severity Score (TTSS). CONCLUSION: Our study demonstrates that serum ACE levels are increased in thoracic trauma patients with higher levels, indicating the severe nature of trauma in concordance with increased TTSS scores.


Assuntos
Peptidil Dipeptidase A/metabolismo , Traumatismos Torácicos/enzimologia , Acidentes por Quedas , Acidentes de Trânsito , Adolescente , Adulto , Idoso , Biomarcadores , Feminino , Humanos , Escala de Gravidade do Ferimento , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Peptidil Dipeptidase A/sangue , Prognóstico , Estudos Prospectivos , Síndrome do Desconforto Respiratório do Adulto/diagnóstico , Síndrome do Desconforto Respiratório do Adulto/enzimologia , Traumatismos Torácicos/diagnóstico , Traumatismos Torácicos/etiologia , Traumatismos Torácicos/mortalidade , Ferimentos Penetrantes/complicações , Adulto Jovem
2.
Urologiia ; (2): 73-81, 2019 Jun.
Artigo em Russo | MEDLINE | ID: mdl-31162906

RESUMO

Prostate cancer (PCa) is the 4th most commonly diagnosed cancer in the male population and incidence of different stages is increasing every year. The efficiency of PCa treatment is strongly dependent on the its stage. Prostate Specific Antigen (PSA) is the most widely used and universal biomarker of PCa worldwide. Considering its limited predictive value, particularly in patients older than 50 with PSA level ranging from 4.5 to 10 ng/ml, there is a need to introduce new serum biomarkers of PCa. Current data on different PCa biomarkers are reviewed in the article as well as a role of angiotensin-converting enzyme (ACE) as a novel PCa biomarker.


Assuntos
Biomarcadores Tumorais/sangue , Peptidil Dipeptidase A/sangue , Hiperplasia Prostática/sangue , Neoplasias da Próstata/sangue , Humanos , Masculino , Próstata/metabolismo , Antígeno Prostático Específico/sangue , Hiperplasia Prostática/diagnóstico , Neoplasias da Próstata/diagnóstico
3.
Oxid Med Cell Longev ; 2019: 5360560, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31182993

RESUMO

Currently, the therapeutic strategy against metabolic syndrome and its complications is required due to the increasing prevalence and its impact. Due to the benefits of both mulberry fruit extract and encapsulation technology, we hypothesized that encapsulated mulberry fruit extract (MME) could improve metabolic parameters and its complication risk in postmenopausal metabolic syndrome. To test this hypothesis, female Wistar rats were induced experimental menopause with metabolic syndrome by bilateral ovariectomy (OVX) and high-carbohydrate high-fat (HCHF) diet. Then, they were orally given MME at doses of 10, 50, and 250 mg/kg BW for 8 weeks and the parameters, such as percentage of body weight gain, total cholesterol, triglycerides, HDL-C, LDL-C, atherogenic index, fasting blood glucose, plasma glucose area under the curve, serum angiotensin-converting enzyme (ACE), oxidative stress status, histology, and protein expression of PPAR-γ, TNF-α, and NF-κB in adipose tissues were determined. MME improved body weight gain, adiposity index, glucose intolerance, lipid profiles, atherogenic index, ACE, oxidative stress status, and protein expression of TNF-α and NF-κB. Moreover, MME attenuated adipocyte hypertrophy and enhanced PPAR-γ expression. Taken altogether, MME decreased metabolic syndrome and its complication via the increased PPAR-γ expression. Therefore, MME is the potential candidate for improving metabolic syndrome and its related complications. However, further research in clinical trial is still necessary.


Assuntos
Frutas/química , Menopausa/efeitos dos fármacos , Síndrome Metabólica/tratamento farmacológico , Morus/química , Extratos Vegetais/uso terapêutico , Animais , Dieta Hiperlipídica , Feminino , Síndrome Metabólica/sangue , Síndrome Metabólica/metabolismo , NF-kappa B/metabolismo , Estresse Oxidativo/efeitos dos fármacos , PPAR gama/metabolismo , Peptidil Dipeptidase A/sangue , Ratos Wistar , Fator de Necrose Tumoral alfa/metabolismo
4.
Medicina (Kaunas) ; 55(6)2019 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-31185683

RESUMO

BACKGROUND AND OBJECTIVES: The ACE gene encodes the angiotensin-converting enzyme (ACE), a component of the renin-angiotensin system. Increased ACE activity may cause abnormal regulation of placental circulation and angiogenesis, resulting in adverse pregnancy outcomes. Previous studies have reported that the insertion/deletion (I/D) polymorphism of the ACE gene is associated with the development of preterm birth (PTB). However, results of the association between ACE gene I/D and PTB are inconsistent in various populations. Therefore, we performed a case-control study and a meta-analysis to evaluate the association between ACE I/D polymorphism and PTB. Materials and Methods: We analyzed a total of 254 subjects (111 patients with PTB and 143 women at ≥38 weeks gestation) for the case-control study. For the meta-analysis, we searched Google Scholar, PubMed, and NCBI databases with the terms "ACE," "angiotensin-converting enzyme," "preterm birth," "preterm delivery," and their combinations. Results: Our results of the case-control study indicated that ACE I/D polymorphism is significantly associated with PTBs in the overdominant genetic model (odds ratio (OR) 0.57, 95% confidence interval (CI) 0.347-0.949, p = 0.029) and that the ID genotype of ACE I/D polymorphism has a protective effect for PTB (OR 0.57, 95% CI 0.333-0.986, p = 0.043). Similarly, the meta-analysis showed that the OR for the ACE gene ID genotype was 0.66 (95% CI 0.490-0.900, p < 0.01). Conclusion: The ACE gene ID genotype has a significant association with PTB and is a protective factor for PTB. A larger sample set and functional studies are required to further elucidate of our findings.


Assuntos
Peptidil Dipeptidase A/análise , Nascimento Prematuro/sangue , Adulto , Estudos de Casos e Controles , Feminino , Predisposição Genética para Doença/epidemiologia , Humanos , Recém-Nascido , Razão de Chances , Peptidil Dipeptidase A/sangue , Polimorfismo Genético/fisiologia , Nascimento Prematuro/epidemiologia , República da Coreia/epidemiologia
5.
J Vet Intern Med ; 33(4): 1571-1584, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31254308

RESUMO

BACKGROUND: Angiotensin-converting enzyme 2 (ACE2) is a homologue of angiotensin-converting enzyme (ACE) and produces angiotensin peptides (APs), such as angiotensin 1-9 and 1-7 that are vasodilatory and natriuretic, and act to counterbalance angiotensin II. HYPOTHESIS: Evidence of ACE2 can be found in tissues and plasma of dogs. Equilibrium concentrations of renin angiotensin aldosterone system (RAAS) APs differ in dogs with heart disease compared to healthy dogs and recombinant human ACE2 (rhACE2) alters relative concentrations of APs. ANIMALS: Forty-nine dogs with and 34 dogs without heart disease. METHODS: Immunohistochemistry and assays for tissue and plasma ACE2 activity and equilibrium concentrations of plasma RAAS APs were performed. RESULTS: Immunolabeling for ACE2 was present in kidney and myocardial tissue. Median plasma ACE2 activity was significantly increased in dogs with congestive heart failure (CHF; 6.9 mU/mg; interquartile range [IQR], 5.1-12.1) as compared to control (2.2 mU/mg; IQR, 1.8-3.0; P = .0003). Plasma equilibrium analysis of RAAS APs identified significant increases in the median concentrations of beneficial APs, such as angiotensin 1-7, in dogs with CHF (486.7 pg/mL; IQR, 214.2-1168) as compared to those with preclinical disease (41.0 pg/mL; IQR, 27.4-45.1; P < .0001) or control (11.4 pg/mL; IQR, 7.1-25.3; P = .01). Incubation of plasma samples from dogs with CHF with rhACE2 increased beneficial APs, such as angiotensin 1-9 (preincubation, 10.3 pg/mL; IQR, 4.4-37.2; postincubation, 2431 pg/mL; IQR, 1355-3037; P = .02), while simultaneously decreasing maladaptive APs, such as angiotensin II (preincubation, 53.4 pg/mL; IQR, 28.6-226.4; postincubation, 2.4 pg/mL; IQR, 0.50-5.8; P = .02). CONCLUSIONS AND CLINICAL IMPORTANCE: Recognition of the ACE2 system expands the conventional view of the RAAS in the dog and represents an important potential therapeutic target.


Assuntos
Angiotensinas/sangue , Cardiopatias/veterinária , Peptidil Dipeptidase A/sangue , Animais , Estudos de Casos e Controles , Cães , Feminino , Cardiopatias/sangue , Cardiopatias/enzimologia , Insuficiência Cardíaca/sangue , Insuficiência Cardíaca/enzimologia , Insuficiência Cardíaca/veterinária , Imuno-Histoquímica , Rim/enzimologia , Masculino , Miocárdio/enzimologia , Peptídeos/sangue , Peptidil Dipeptidase A/análise , Sistema Renina-Angiotensina
6.
Mar Drugs ; 17(5)2019 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-31086041

RESUMO

The peptide QAGLSPVR, which features high angiotensin-I-converting enzyme (ACE) inhibitory activity, was identified in our previous study. In this study, the in vivo antihypertensive effect of QAGLSPVR was evaluated. Results showed that QAGLSPVR exerts a clear antihypertensive effect on spontaneously hypertensive rats (SHRs), and the systolic and diastolic blood pressures of the rats remarkably decreased by 41.86 and 40.40 mm Hg, respectively, 3 h after peptide administration. The serum ACE activities of SHRs were determined at different times, and QAGLSPVR was found to decrease ACE activities in serum; specifically, minimal ACE activity was found 3 h after administration. QAGLSPVR could be completely absorbed by the Caco-2 cell monolayer, and its transport percentage was 3.5% after 2 h. The transport route results of QAGLSPVR showed that Gly-Sar and wortmannin exert minimal effects on the transport percentage of the peptide (p> 0.05), thus indicating that QAGLSPVR transport through the Caco-2 cell monolayer is not mediated by peptide transporter 1 or transcytosis. By contrast, cytochalasin D significantly increased QAGLSPVR transport (p< 0.05); thus, QAGLSPVR may be transported through the Caco-2 cell monolayer via the paracellular pathway.


Assuntos
Inibidores da Enzima Conversora de Angiotensina/farmacologia , Anti-Hipertensivos/farmacologia , Pressão Sanguínea/efeitos dos fármacos , Oligopeptídeos/farmacologia , Inibidores da Enzima Conversora de Angiotensina/farmacocinética , Animais , Anti-Hipertensivos/farmacocinética , Células CACO-2 , Captopril/farmacologia , Humanos , Hipertensão/sangue , Hipertensão/tratamento farmacológico , Hipertensão/metabolismo , Masculino , Oligopeptídeos/farmacocinética , Peptidil Dipeptidase A/sangue , Ratos Endogâmicos SHR
7.
Dis Markers ; 2019: 8565423, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30944672

RESUMO

Purpose: Sarcoidosis is a systemic granulomatous disease with unknown etiology. Many clinical presentations have been reported, and acute disease needs to be distinguished from subacute and chronic disease. The unpredictable clinical course of the disease prompted us to evaluate the clinical utility of biomarker serum detection in sarcoidosis follow-up. Methods: Serum concentrations of chitotriosidase, ACE, KL-6, and lysozyme were analyzed by different methods in a population of 74 sarcoidosis patients (46 on steroid therapy at sampling) regularly monitored at Siena Sarcoidosis Regional Referral Centre and in a group of controls with the aim of comparing their contribution to clinical management of sarcoidosis patients. Results: KL-6 concentrations were significantly elevated in sarcoidosis patients with lung fibrosis and were significantly correlated with DLco and CPI score, while chitotriosidase was significantly higher in patients with extrapulmonary localizations. With a cut-off value of 303.5 IU/ml, KL-6 showed the best sensitivity (78%), while chitotriosidase reported the best specificity (85%) among the biomarkers. Conclusions: KL-6 is a reliable biomarker of fibrotic lung involvement in sarcoidosis patients. Among biomarkers, KL-6 showed the best sensitivity and serum chitotriosidase the best specificity, even in patients on chronic steroid therapy, and seemed to correlate with extrapulmonary localizations.


Assuntos
Hexosaminidases/sangue , Pneumopatias/sangue , Mucina-1/sangue , Muramidase/sangue , Peptidil Dipeptidase A/sangue , Sarcoidose/sangue , Adulto , Biomarcadores/sangue , Feminino , Hexosaminidases/normas , Humanos , Masculino , Pessoa de Meia-Idade , Muramidase/normas , Peptidil Dipeptidase A/normas , Sensibilidade e Especificidade
8.
Int Urol Nephrol ; 51(4): 755-764, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30734886

RESUMO

PURPOSE: Podocytes are terminally differentiated cells lining the Bowman's capsule. Podocytes are critical for the proper glomerular filtration barrier function. At the same time, autophagy is crucial for maintaining podocyte homeostasis and insufficient autophagy could cause podocyte loss and proteinuria that is commonly observed in diabetic nephropathy (DN). METHODS: In this study, we investigated the role of spironolactone in podocyte loss and autophagy. DN model was established in male Sprague-Dawley rats using high-fat diet and low-dose streptozotocin. The impact of spironolactone on metabolic and biochemical parameters were tested by automatic biochemical analyzer. The angiotensin converting enzyme 1 and 2 (ACE1 and ACE2) and aldosterone were examined by ELISA. We examined the kidney histology and autophagy in podocytes by histochemical staining and electron microscopy. Podocyte loss and autophagy were analyzed by anti-NPHS2 and anti-WT1 as well as anti-Beclin1 and anti-LC3B, respectively. RESULTS: Spironolacton decreased the urinary albumin excretion, lipids and fasting glucose levels, and alleviated kidney damage. Further, spironolactone increased the expression of the podocyte-specific markers WT1 and NPHS2, as well as the autophagic markers Beclin1 and LC3B (P < 0.05). Additionally, spironolactone partially blocked the rennin angiotensin aldosterone system (RAAS) by regulating the ACE1, ACE2 and aldosterone levels. CONCLUSIONS: In conclusion, spironolactone promoted autophagy in podocytes and further alleviated DN through partially blocking the RAAS.


Assuntos
Autofagia/efeitos dos fármacos , Nefropatias Diabéticas/tratamento farmacológico , Antagonistas de Receptores de Mineralocorticoides/uso terapêutico , Podócitos , Espironolactona/uso terapêutico , Albuminúria/tratamento farmacológico , Aldosterona/metabolismo , Animais , Proteína Beclina-1/metabolismo , Diabetes Mellitus Experimental/induzido quimicamente , Nefropatias Diabéticas/metabolismo , Nefropatias Diabéticas/patologia , Modelos Animais de Doenças , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Masculino , Proteínas de Membrana/metabolismo , Proteínas Associadas aos Microtúbulos/metabolismo , Antagonistas de Receptores de Mineralocorticoides/farmacologia , Peptidil Dipeptidase A/sangue , Sistema Renina-Angiotensina/efeitos dos fármacos , Espironolactona/farmacologia , Proteínas WT1/metabolismo
9.
Lupus ; 28(2): 223-233, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30621494

RESUMO

BACKGROUND: There are no reports about the association of angiotensin II type 2 receptor ( AT2R) gene polymorphisms and susceptibility to systemic lupus erythematosus (SLE) in children. OBJECTIVE: The objective of this research is to study AT2R gene polymorphisms in exon 3 (C1593A) and intron 1 (A1675G) in Egyptian children with SLE and its correlation with disease manifestations and serum angiotensin-converting enzyme (ACE) level. METHODS: Typing of AT2R gene polymorphisms was conducted in 123 children with SLE in comparison with 100 healthy controls using the restriction fragment length polymorphism method. RESULTS: Significant differences were found between SLE patients and controls for A-containing genotypes (CA + AA) and A-allele frequencies of AT2R in exon 3 (C1593A) ( p = 0.01, odds ratio (OR) = 2.5, 95% confidence interval (CI) = 1.3-5.05; p = 0.01, OR = 2.2, 95% CI = 1.2-4.1, respectively). G-containing genotypes (AG + GG) and G allele of AT2R in intron 1 (A1675G) were more frequent in SLE patients compared to controls ( p = 0.01, OR = 2.3, 95% CI = 1.2-4.5; p = 0.02, OR = 2.1, 95% CI = 1.2-3.7, respectively). Serum ACE level was significantly higher in SLE patients than in controls ( p < 0.001). There was no association between AT2R gene polymorphisms and ACE level in serum. Moreover, there was no association between AT2R gene polymorphisms and SLE clinical manifestations. CONCLUSION: AT2R gene polymorphisms can be considered risk factors for SLE development in Egyptian children.


Assuntos
Lúpus Eritematoso Sistêmico/sangue , Lúpus Eritematoso Sistêmico/genética , Peptidil Dipeptidase A/sangue , Receptor Tipo 2 de Angiotensina/genética , Adolescente , Estudos de Casos e Controles , Criança , Estudos Transversais , Egito , Feminino , Frequência do Gene , Predisposição Genética para Doença , Humanos , Masculino , Polimorfismo Genético , Fatores de Risco
10.
Artigo em Inglês | MEDLINE | ID: mdl-30508739

RESUMO

Angiotensin converting enzyme (ACE, peptidyldipeptidase A, EC 3.4.15.1) plays an important role in the regulation of blood pressure. In this study, ACE was purified from human plasma by affinity chromatography in single step. The enzyme purified in 5367-fold from human plasma and specific activity was found to be 1208 EU/mg protein. The purity and molecular weight of ACE were determined by SDS-PAGE, which indicated two bands at around 60 kDa and 70 kDa on the gel. Effect of oxidized glutathione (GSSG) peptide and reduced glutathione (GSH) peptide on purified ACE activity were also investigated in which lisinopril was used as reference inhibitor. GSSG showed activation effect on ACE activity whereas GSH provided inhibition effect. In the lights of activity (%) versus activator graph for GSSG and activity (%) versus inhibitor graphs for GSH and lisinopril; IC50 values for GSH and lisinopril were determined to be 16.2 µM and 0.781 nM, respectively. Type of inhibition for GSH and lisinopril from graph Lineweaver-Burk was found to be reversible non-competitive inhibition and Ki constants for GSH and lisinopril were calculated as 11.7 µM and 0.662 nM, respectively.


Assuntos
Inibidores da Enzima Conversora de Angiotensina/metabolismo , Dissulfeto de Glutationa/metabolismo , Glutationa/metabolismo , Fragmentos de Peptídeos/metabolismo , Peptidil Dipeptidase A , Inibidores da Enzima Conversora de Angiotensina/química , Cromatografia de Afinidade , Glutationa/química , Dissulfeto de Glutationa/química , Humanos , Modelos Lineares , Fragmentos de Peptídeos/química , Peptidil Dipeptidase A/sangue , Peptidil Dipeptidase A/isolamento & purificação , Peptidil Dipeptidase A/metabolismo
11.
Nephron ; 141(1): 61-74, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30326474

RESUMO

BACKGROUND: Decreased levels of leucocytic angiotensin converting enzyme 2 (ACE2) relate to atherosclerosis in patients with chronic kidney disease (CKD). Recently, micro RNA 421 (miR-421) was found to target and down-regulate ACE2 in human cardiac myofibroblasts. In this study, we investigated the correlation between serum levels of miR-421 and ACE2 transcripts in circulating leukocytes of healthy individuals (NP), CKD (3-5) and haemodialysis (HD) patients. Furthermore, we tested the possible interaction between miR-421 and 3'-UTR of ACE2 under normal and uremic milieu. METHODS: The levels of circulating miR-421, serum Ang1-7 and expression of leucocytic ACE2, ACE, MASR, AT1R and AT2R were investigated in 16 CKD3-5 (76 ± 10 years), 32 HD patients (65 ± 13 years) and 23 NP (51 ± 5 years) by employment of specific primers, TaqMan and competitive enzyme-linked immunosorbent assay assays. Interaction between miR-421 and ACE2-3'-UTR was investigated on THP-1 cells by employment of normal and uremic sera, reporter vectors and miR-421 inhibitor. Effects of uremic toxins indoxyl sulphate, p-cresol and p-cresyl sulphate on ACE2 and miR-421 levels were investigated in THP-1 monocytes. RESULTS: The levels of serum miR-421 were significantly elevated, while Ang1-7 was significantly decreased in CKD3-5 and HD patients as compared with NP. Serum Ang1-7 correlated positively with leucocytic ACE2 (r2 = 0.213, p < 0.001). We found a significant and inverse correlation between the levels of circulating miR-421 and the expression of leucocytic ACE2 (r2 = 0.223, p < 0.0001). Further significant and positive correlations could be demonstrated between miR-421 and the transcripts of leucocytic AT1R (r2 = 0.094, p < 0.05) or eGFR (r2 = 0.231, p < 0.0001) or CRP (r2 = 0.092, p < 0.01). We found no correlations between miR-421 and ACE or AT2R or MASR expression. Treatment with miR-421 or uremic serum led to noticeable decrease of reporter 3'UTR-ACE2. Anti-miR-421 treatment resulted in the up-regulation of ACE2 protein. All uremic toxins tested were able to significantly elevate miR-421 levels and decrease the monocytic ACE2 transcripts. CONCLUSIONS: Uremic patients show an enhanced expression of serum miR-421 as compared to healthy individuals. A strong association of circulating miR-421 with decreased transcripts of ACE2 may contribute to the low expression of the enzyme in leukocytes of CKD patients, further supporting the development of atherosclerotic events.


Assuntos
Leucócitos/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , Peptidil Dipeptidase A/sangue , Insuficiência Renal Crônica/sangue , Insuficiência Renal Crônica/genética , Regiões 3' não Traduzidas/genética , Idoso , Idoso de 80 Anos ou mais , Angiotensina I/sangue , Linhagem Celular , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fragmentos de Peptídeos/sangue , Diálise Renal , Uremia/genética , Uremia/metabolismo
12.
Clin Exp Hypertens ; 41(7): 662-669, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30307755

RESUMO

Background: Renin-angiotensin system (RAS) is a complex network of enzymes and peptides with the essential role in blood pressure control. The relationships between RAS components, RAS-related genetic polymorphisms and therapy response in essential hypertension (EH) were widely explored but the results were inconclusive. Aim: The aim of this study was to explore the functional role of ACE insertion/deletion (I/D) polymorphism on the systemic quantity of angiotensin-converting enzyme (ACE), its homolog - ACE2, chymase and angiotensin II in EH patients with respect to achieved therapeutic blood pressure control. Results: Genotyping of ACE I/D polymorphism was performed among 140 patients with EH from Bulgaria. The serological analyses reveal the significant elevation of the serum quantity of all investigated enzymes in EH than normotensive controls. In addition, serum ACE2 (183.57 pg/ml; vs. 151.78 pg/ml; p = 0.02) and chymase (68.5 pg/ml; vs. 23.66 pg/ml; p = 0.034) were significantly higher in patients with uncontrolled EH than controlled EH in response to ACE-inhibitory therapy. Also, ACE I/D polymorphism showed a significant impact on the serum ACE and chymase levels. ACE quantity was the highest among carriers of DD-genotype, followed by ID and II-genotype. Contrary, chymase was in the highest quantity in II-genotype compared to ID-genotype (p = 0.025) and DD-genotype (p = 0.044). Conclusions: Our results suggest that insufficient blood pressure control by ACE-inhibitory therapy could be associated with elevation of serum ACE2 and chymase levels. Also, it appears that ACE I/D polymorphism may influence the circulating quantity of chymase in addition to ACE.


Assuntos
Inibidores da Enzima Conversora de Angiotensina/uso terapêutico , Pressão Sanguínea , Hipertensão Essencial/sangue , Hipertensão Essencial/genética , Peptidil Dipeptidase A/genética , Idoso , Angiotensina II/sangue , Quimases/sangue , Hipertensão Essencial/tratamento farmacológico , Feminino , Genótipo , Humanos , Mutação INDEL , Masculino , Pessoa de Meia-Idade , Peptidil Dipeptidase A/sangue , Polimorfismo Genético , Sistema Renina-Angiotensina
13.
Intern Med ; 58(5): 679-684, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30449791

RESUMO

A 61-year-old man was diagnosed with sarcoidosis involving the lungs, eyes, parotid gland and extrathoracic lymph nodes complicated by chronic kidney injury and hypercalcemia. Kidney biopsy showed non-specific interstitial nephritis and nephrosclerosis. However, immunohistochemical staining of cell surface markers revealed a multinucleated giant macrophage surrounded by T-cells, suggesting granulomatous interstitial nephritis. Corticosteroid improved the kidney function, and reduced the serum levels of calcium and angiotensin-converting enzyme. Sarcoid nephropathy may be caused by the combination of several sarcoidosis-associated pathophysiological conditions and a comprehensive kidney examination should be performed to assess the type of injury when determining a treatment strategy.


Assuntos
Nefrite Intersticial/etiologia , Sarcoidose/complicações , Biomarcadores/sangue , Biópsia , Cálcio/sangue , Glucocorticoides/uso terapêutico , Humanos , Hipercalcemia/etiologia , Rim/patologia , Masculino , Pessoa de Meia-Idade , Nefrite Intersticial/sangue , Nefrite Intersticial/tratamento farmacológico , Nefrite Intersticial/patologia , Nefroesclerose/sangue , Nefroesclerose/etiologia , Nefroesclerose/patologia , Peptidil Dipeptidase A/sangue , Cintilografia , Sarcoidose/sangue , Sarcoidose/tratamento farmacológico , Sarcoidose/patologia
15.
Indian J Dermatol Venereol Leprol ; 85(3): 295-299, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-29582789

RESUMO

Background: Alopecia areata is an immune-dependent disorder characterized by the interaction of T-lymphocytes with follicular antigens. Recent studies have shown the existence of a local renin-angiotensin system in the skin, where angiotensin-converting enzyme (ACE) plays a role in autoimmunity and inflammation. Aim: The objective of this study was to evaluate serum and tissue ACE activity in patients with alopecia areata. Methods: This case-control study was conducted on patients with alopecia areata and healthy controls. Serum and tissue ACE activity were assessed and compared between the two groups. Results: Twenty-five alopecia areata patients (60% male, mean age 32.1 ± 9.9 years) and 24 controls (50% male, mean age 37.4 ± 8.8 years) were included. Mean serum ACE activity was 52.1 ± 9 U/L in cases and 55.3 ± 14.7 U/L in controls (P = 0.37). Tissue ACE activity was significantly lower in cases in all parts of the skin i.e. epidermis (P = 0.016), follicular epithelium (P = 0.004), and endothelium (P = 0.037). Among cases, serum ACE activity was significantly higher in patients with more severe disease (P = 0.030), nonpatchy alopecia areata (alopecia universalis; ophiasis, patchy and ophiasis, diffuse) (P = 0.029), and with nail involvement (P = 0.027). Limitations: The sample size was too small to draw definite conclusions. Further, most of the patients had only mild or moderate alopecia areata. Conclusion: Unlike in some other inflammatory diseases, the tissue level of ACE seems to be significantly lower in alopecia areata compared to normal controls. Serum ACE was significantly higher in patients with more severe disease.


Assuntos
Alopecia em Áreas/sangue , Alopecia em Áreas/diagnóstico , Peptidil Dipeptidase A/sangue , Distribuição Tecidual/fisiologia , Adulto , Alopecia em Áreas/metabolismo , Biomarcadores/sangue , Biomarcadores/metabolismo , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Peptidil Dipeptidase A/metabolismo , Adulto Jovem
16.
J Affect Disord ; 244: 67-70, 2019 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-30321766

RESUMO

BACKGROUND: Abnormal activity of two enzymes relevant to neurodevelopment, namely nuclear-distribution element-like 1 (Ndel1) and angiotensin I-converting enzyme (ACE), was reported in individuals with schizophrenia; to our knowledge, these oligopeptidases were never measured in bipolar disorder (BD). AIMS: Evaluate the enzyme activity of Ndel1 and ACE in euthymic individuals with BD type 1 which was compare to healthy control (HC) group. METHODS: Ndel1 and ACE activities were assessed in the serum of individuals with BD type 1 according to DSM-IV criteria (n = 70) and a HC group (n = 34). The possible differences between BD type 1 and HC groups were evaluated using Analysis of Covariance (ANCOVA), and the results were adjusted for age, gender and body mass index. RESULTS: We observed a positive correlation between Ndel1 activity and the total YMRS score in BD group (p = 0.030) and a positive correlation between ACE activity and Ham-D score (p = 0.047). ANCOVA analysis showed lower Ndel1 activity in BDs compared to HCs. Interestingly, we did not observe between-groups differences in ACE activity, despite the recognized correlation of ACE activity levels with cognitive functions, also described to be worsened in psychiatric patients. CONCLUSION: Oligopeptidases, especially Ndel1, which has been strongly correlated with neurodevelopment and brain formation, are potentially a good new target in the study of the neurobiology of BD. LIMITATIONS: The relatively small sample size did not permit to examine the cause-effect relationship of clinical dimensions of BD and the enzymatic activity.


Assuntos
Transtorno Bipolar/sangue , Transtorno Bipolar/enzimologia , Proteínas de Transporte/sangue , Peptidil Dipeptidase A/sangue , Adolescente , Adulto , Estudos de Casos e Controles , Manual Diagnóstico e Estatístico de Transtornos Mentais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
17.
Nutrients ; 10(12)2018 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-30469459

RESUMO

Hypertension is the leading risk factor for cardiovascular disease, and prevention of high blood pressure through diet and lifestyle should be a preferred approach. High intake of fish is associated with lower blood pressure, possibly mediated through the proteins since peptides with angiotensin-converting enzyme (ACE) inhibiting capacities have been identified in fish skin, backbone, and fillet. The effects of cod meals made from residual materials and fillet on blood pressure were investigated in obese Zucker fa/fa rats which spontaneously develop high blood pressure. Rats were fed diets containing water-soluble (stickwater) or water-insoluble (presscake) fractions of protein-rich meals from cod residual materials (head, gut, backbone with muscle residuals, skin, trimmings) or fillet. Rats were fed diets containing 25% of total protein from cod meal and 75% of protein from casein, or casein as the sole protein source (control group) for four weeks. Results show that a diet containing residual presscake meal with high gut content prevented blood pressure increase, and this cod residual meal also showed the strongest in vitro inhibitions of ACE and renin activities. In conclusion, a diet containing water-insoluble proteins (presscake meal) with high gut content prevented increase in blood pressure in obese Zucker fa/fa rats.


Assuntos
Pressão Sanguínea/efeitos dos fármacos , Proteínas de Peixes da Dieta/farmacologia , Gadiformes , Hipertensão/prevenção & controle , Peptidil Dipeptidase A/sangue , Renina/sangue , Alimentos Marinhos , Animais , Dieta , Hipertensão/sangue , Masculino , Obesidade/complicações , Obesidade/tratamento farmacológico , Ratos Zucker
18.
Pan Afr Med J ; 30: 67, 2018.
Artigo em Francês | MEDLINE | ID: mdl-30344851

RESUMO

We report the case of a 37-year old patient with right optic neuropathy. Magnetic resonance imaging (MRI) showed T2 hypersignal in the midline, enhanced after gadolinium injection (figure 1). Cerebrospinal fluid (CSF) analysis revealed lymphocytic meningitis with 64 white blood cells associated with hyperproteinorachy. Chest CT scan suggested the diagnosis of granulomatous inflammation, namely Stage 2 sarcoidosis. The level of serum angiotensin-converting enzyme (ACE) was high. The patient underwent corticosteroid therapy with good outcome. Sarcoidosis is a granulomatous diffuse, multisystemic disorder of unknown etiology. Neurologic sarcoidosis occurs in 5-15% of cases and neurologic symptoms suggest it in 10-30% of cases. Meningoencephalitis and cranial nerve involvement are the most common cliniconeurological manifestations, that are very varied. Facial nerve is the most common nerve to be affected, followed by the optic nerve. Brain MRI can better identify brain lesions, manifesting as infiltrating nodules in T1-weighted hyposignal and in T2-weighted hypersignal enhanced after contrast injection. It mainly affects the suprasellar region with involvement of the hypothalamus, the pituitary peduncle and the optic chiasm. Other anomalies are easily identified by gadolinium contrast agent, including diffuse or nodular thickening of the leptomeninges manifesting as pachymeningitis and lesions in the brain parenchyma (parietal, frontal, cerebellar regions) or in the spinal cord. The diagnosis is based on combination of clinical, radiological, laboratory tests and on histological data. Treatment is based on corticotherapy as first-line therapy sometimes associated with immunosuppressants.


Assuntos
Corticosteroides/administração & dosagem , Doenças do Sistema Nervoso Central/diagnóstico , Doenças do Nervo Óptico/diagnóstico , Sarcoidose/diagnóstico , Adulto , Doenças do Sistema Nervoso Central/diagnóstico por imagem , Doenças do Sistema Nervoso Central/tratamento farmacológico , Meios de Contraste/administração & dosagem , Humanos , Imagem por Ressonância Magnética/métodos , Masculino , Doenças do Nervo Óptico/diagnóstico por imagem , Doenças do Nervo Óptico/tratamento farmacológico , Peptidil Dipeptidase A/sangue , Sarcoidose/diagnóstico por imagem , Sarcoidose/tratamento farmacológico , Tomografia Computadorizada por Raios X
20.
Medicine (Baltimore) ; 97(42): e12917, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30335025

RESUMO

Angiotensin-converting enzyme 2 (ACE2) plays an important role in the development of essential hypertension (EH). The aim of this study was to investigate the relationship of ACE2 gene polymorphisms and enzymatic activity with EH in the northeastern Chinese Han population. 34 single-nucleotide polymorphism (SNP) loci of ACE2 were detected in 1024 EH patients and 956 normotensive (NT) controls by Sequenom Mass-ARRAY RS1000. Five SNPs (rs1514283, rs4646155, rs4646176, rs2285666, and rs879922) in ACE2 gene were determined to significantly associate with EH in female participants, while no SNP locus was linked to male group. Specifically, it was the first time to report that rs4646155 was significantly associated with EH in females. Furthermore, the correlation between ACE2 activity and clinical parameters were performed by Pearson correlation analysis in EH patients. We found that the ACE2 activity level was negatively correlated with body mass index (BMI), DBP, and pulse pressure, and significantly positively with ACE2 concentration, blood glucose and estrogen level in female EH patients. These results demonstrated that the genetic variants of ACE2 played vital roles in the development of EH. And the serum ACE2 activity can predict the development of cardiac dysfunction in EH patients.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Hipertensão Essencial/genética , Peptidil Dipeptidase A/sangue , Polimorfismo de Nucleotídeo Único , Fatores Sexuais , Idoso , Pressão Sanguínea/genética , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Peptidil Dipeptidase A/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA