Characterization and the mechanism underlying the cryoprotective activity of a peptide from large yellow croaker (Pseudosciaena crocea).

Ice crystal-induced protein denaturation is the main cause of the deterioration of fish during frozen storage and transportation. In this study, the ultra-performance liquid chromatography - quadrupole - time of flight (UPLC-Q-TOF) technique was used to identify and screen tryptic peptides Ile-Glu-Glu-Leu-Glu-Glu-Leu-Glu-Ala-Glu-Arg (IEELEEELEAER) from large yellow croaker (Pseudosciaena crocea). The results were used study their cryoprotective effects on turbot fish meat during freeze-thaw cycles at different concentrations, and to investigate their anti-freezing mechanism. The results showed that the I-2.0 group effectively inhibiting the degeneration and structure changes of myofibrillar proteins after three freeze-thaw cycles, and the Ca2+-ATPase activity (1.65 µmolPi/mg/h), increased by 55.86% compared with that of the control group. Additionally, peptide IEELEEELEAER could provide antifreeze protection by binding to the surface of ice crystals and inhibiting their transformation. This peptide acts as a natural cryoprotectant and might be used for the cryogenic storage and transportation of fish products.

Links between reproduction and immunity in two sympatric wild marine fishes.

According to life-history theory, limited resources can result in trade-offs between costly physiological functions. Particularly, it can be expected that individuals present lower immune function, or an alternative immunological strategy, during their reproductive compared to their non-reproductive season. Here we investigate the link between reproduction and immunity in two sympatric marine fish species, the rockfish Sebastes oculatus and the sandperch Pinguipes brasilianus. The results showed lower values of total white blood cells and spleen index, but higher levels of natural antibodies (only in females) in reproductive rockfish compared to non-reproductive ones. On the other hand, reproductively active sandperch showed lower levels of natural antibodies and a higher neutrophil to lymphocyte ratio and spleen index (only in males), compared to non-reproductive ones. Also, negative correlations between reproductive and immune parameters were observed in female rockfish at the individual level, but not in sandperch. Our results are consistent with the presence of different immunological strategies in reproductive and non-reproductive periods, with patterns that appear to be species-specific. This specificity suggests that various aspects of immunity might respond differentially to resource limitation, which could be associated with the disparate life-history strategies of the studied species. Alternatively, though not exclusively, the observed patterns could be driven by abiotic factors that characterize the reproductive season of each species (i.e., winter for rockfish, summer for sandperch). Our study contributes to ecoimmunological knowledge on free-living fish and highlights that detection of trade-offs can depend on the combination of study species, season, sex, and specific immune components measured.

Organic UV absorbents in the deepwater redfish (Sebastes mentella) from the St. Lawrence Estuary and Gulf: Distribution and human health risk assessment.

UV absorbents (UVAs), such as organic UV filters (UVFs) and benzotriazole UV stabilizers (BZT-UVs), are used in a wide range of consumer and industrial products and they are contaminants of emerging concern in the environment. However, their occurrence and fate in the deep-sea environments are inadequately understood. This study investigated the occurrence and distribution of five UVFs and ten BZT-UVs in the muscle (n = 127) of 2019-collected deepwater redfish (Sebastes mentella) from the St. Lawrence Estuary and Gulf (SLEG) (Canada) to better understand the accumulation of these contaminants in deep-sea fish. Small redfish (<30 cm) tended to have higher concentrations of UVAs in the muscle than that of larger specimens (>30 cm). The UVF 2-hydroxy-4-methoxybenzophenone (BP3) was the most frequently detected (present in 34 % of all samples) target UVA, with concentrations as high as 413 ng/g (dry weight). According to the δ15N and δ13C data, pelagic-eating redfish, and individuals with lower trophic levels had higher lipid content and accumulated more BP3 in their muscles. Four BZT-UVs were detected in redfish muscle, but the detection frequency was lower than 30 %. The estimated hazard quotient for these contaminants was <2.3 × 10-2 for general Canadian populations, indicating that they are unlikely to pose health risks to humans through redfish consumption. Factors influencing UVAs bioaccumulation in redfish, as well as the effects UVAs may have on deep-sea species, should be researched further.

Tissue-specific transcriptional response of post-larval clownfish to ocean warming.

Anthropogenically driven climate change is predicted to increase average sea surface temperatures, as well as the frequency and intensity of marine heatwaves in the future. This increasing temperature is predicted to have a range of negative physiological impacts on multiple life-stages of coral reef fish. Nevertheless, studies of early-life stages remain limited, and tissue-specific transcriptomic studies of post-larval coral reef fish are yet to be conducted. Here, in an aquaria-based study we investigate the tissue-specific (brain, liver, muscle, and digestive tract) transcriptomic response of post-larval (20 dph) Amphiprion ocellaris to temperatures associated with future climate change (+3 °C). Additionally, we utilized metatranscriptomic sequencing to investigate how the microbiome of the digestive tract changes at +3 °C. Our results show that the transcriptional response to elevated temperatures is highly tissue-specific, as the number of differentially expressed genes (DEGs) and gene functions varied amongst the brain (102), liver (1785), digestive tract (380), and muscle (447). All tissues displayed DEGs associated with thermal stress, as 23 heat-shock protein genes were upregulated in all tissues. Our results indicate that post-larval clownfish may experience liver fibrosis-like symptoms at +3 °C as genes associated with extracellular matrix structure, oxidative stress, inflammation, glucose transport, and metabolism were all upregulated. We also observe a shift in the digestive tract microbiome community structure, as Vibrio sp. replace Escherichia coli as the dominant bacteria. This shift is coupled with the dysregulation of various genes involved in immune response in the digestive tract. Overall, this study highlights post-larval clownfish will display tissue-specific transcriptomic responses to future increases in temperature, with many potentially harmful pathways activated at +3 °C.

Gelatin nanofibers with black elderberry, Au nanoparticles and SnO2 as intelligent packaging layer used for monitoring freshness of Hake fish.

The electrospun gelatin nanofibers containing black elderberry (BE) extract, Au nanoparticles (AuNPs) and SnO2 were fabricated as intelligent packaging layer for Hake fish (Merluccius merluccius) fillets. Image analysis confirmed the formation of continuous ultrafine fibers. Differences between nanofibers were evaluated in terms of thermal stability, and chemical composition during storage. Fourier transform infrared spectrums indicated strong bonding interactions between gelatin and other compounds. Thermal analysis results showed that the addition of AuNPs contributed to the thermal stabilization of the gelatin chain. L, a, and b values of nanofibers were also measured. A rapid color change occurred after exposure to volatiles with the highest difference in L (52.29 %) of the sample containing gelatin, BE, SnO2 and AuNPs (p < 0.05). This study showed that the absorption of volatiles on nanofibers can be detected from color changes of nanofibers. The outcomes of this study can be applied for intelligent packaging layer in seafood products.

Daily thermal variability does not modify long-term gene expression relative to stable thermal environments: A case study of a tropical fish.

Global warming is leading to an increase in the frequency and intensity of extreme weather events, magnifying the breadth of temperatures faced by ectotherms across days and seasons. Despite the importance and ecological relevance of diurnal thermal variability, the vast majority of knowledge on gene expression patterns and physiology stems from animals acclimated to constant temperatures or in the early stages of exposure to a new temperature regime. If heterothermal environments modulate responses differently from constant thermal environments, our existing capacity to forecast impacts of climate warming may be compromised. To address this knowledge gap, we acclimated barramundi (Lates calcarifer) to 23 °C, 29 °C (optimal), 35 °C and to thermal cycling conditions (23-35 °C daily with a mean of 29 °C) and sampled liver and white muscle tissue before acclimation and after 2 and 17 weeks of acclimation. NanoString nCounter technologies were used to measure expression of 20 genes related to metabolism, growth and maintenance of cellular homeostasis. Acclimation to cool and warm conditions caused predictable changes in whole-animal performance (metabolism and growth) and the underlying gene expression patterns. Acclimation to a cycling temperature regime did not change the molecular regulation of metabolism or growth compared with barramundi acclimated to constant 29 °C, nor did it cause any discernible effects on whole-animal performance. However, the heat shock response was higher in the former group, suggesting that barramundi under a daily temperature cycle have an increased need for cellular chaperoning to minimise detrimental effects of temperature on proteins. We conclude that the genetic regulation of metabolism and growth may be more dependent on the mean daily temperature than on the daily temperature range.

What kind of sheets does the fat sleeper fish prefer? Effect of pond bottom color on the growth and health of Dormitator latifrons (Richardson 1844).

A little-studied characteristic of the Mexican native fish Dormitator latifrons is the effect that the color of the bottom or lining of ponds or tubs can have on their growth and blood parameters under controlled conditions. After a quarantine period in prophylactic treatment, an initial assay was performed. The organisms were grouped into four treatments (white, yellow, blue, and black) in triplicate, with 30 individuals with an average weight of 34.7± 2.5 g and average size of 12.5 ± 1.5 cm per tank. The trial lasted 60 days, after which a final biometry to all specimens and blood extractions to six random organisms per tank were performed. The following hematological and blood chemistry parameters were evaluated: erythrocytes, cell counts, and differential counts, as well as glucose, albumin, a/g ratio, and total proteins. Final weight, weight gain, and specific growth rate presented statistical differences between treatments (p<0.05), with dark bottoms (blue and black) above 80% of WG and above 1.0 of SGR. No statistically differences were found in hematological blood chemistry parameters (p>0.05). The growth results suggest that dark pond bottoms promote the adaptation of Dormitator latifrons by allowing it to avoid detection by predators through the adoption of a cryptic coloration. However, the species shows a great capacity for crypsis, being able to change its pigmentation to adapt to different bottom colors.

Morphological and molecular characterization of Encotyllabe vallei Monticelli, 1907 (Monopisthocotylea, Monogenea) from the gilthead seabream Sparus aurata Linnaeus (Teleostei, Sparidae) from the southwestern Mediterranean and notes on host specificity of the genus Encotyllabe Diesing, 1850.

Incomplete original descriptions, the unavailability or poor conditions of specimens and the lack of detailed redescriptions have caused the validity of several species of the genus Encotyllabe Diesing, 1850 to be questioned. To date, seven of the recognized species were described upon one or two specimens, hindering study of intraspecific variations. This was made worse by considering few morphoanatomical differences sufficient to erect new species. Among Encotyllabe spp. occurring in Mediterranean waters, E. vallei was first described from the gilt-head bream Sparus aurata (Sparidae) off Italy. Although beautifully illustrated for a paper from that century, morphometric data for E. vallei from the type-host S. aurata remain unavailable. Previous records of E. vallei provided either morphometrical or molecular data, and its validity was questioned. We provide a redescription of E. vallei based on newly collected specimens from the S. aurata from the southwestern Mediterranean (off Algeria) using integrative taxonomy. Analysis of cox1 sequences of E. vallei from S. aurata, compared to sequences from other sparid hosts, mainly Pagellus bogaraveo, revealed a divergence not exceeding 2%, suggesting a stenoxenic specificity for this monogenean. Given that P. bogaraveo is the type-host for Encotyllabe pagelli, we were tempted to suggest a synonymy between E. vallei and E. pagelli. We refrained from doing so because E. pagelli was first described from the Atlantic coast off Brest, France. Morphological data for Encotyllabe from P. bogaraveo are warranted assessing the host specificity of E. vallei and whether there might be a species complex within individual sparid fish species.

Host-microbiota-parasite interactions in two wild sparid fish species, Diplodus annularis and Oblada melanura (Teleostei, Sparidae) over a year: a pilot study.

BACKGROUND: The microbiota in fish external mucus is mainly known for having a role in homeostasis and protection against pathogens, but recent evidence suggests it is also involved in the host-specificity of some ectoparasites. In this study, we investigated the influence of seasonality and environmental factors on both fish external microbiota and monogenean gill ectoparasites abundance and diversity and assessed the level of covariations between monogenean and bacterial communities across seasons. To do so, we assessed skin and gill microbiota of two sparid species, Oblada melanura and Diplodus annularis, over a year and collected their specific monogenean ectoparasites belonging to the Lamellodiscus genus. RESULTS: Our results revealed that diversity and structure of skin and gill mucus microbiota were strongly affected by seasonality, mainly by the variations of temperature, with specific fish-associated bacterial taxa for each season. The diversity and abundance of parasites were also influenced by seasonality, with the abundance of some Lamellodiscus species significantly correlated to temperature. Numerous positive and negative correlations between the abundance of given bacterial genera and Lamellodiscus species were observed throughout the year, suggesting their differential interaction across seasons. CONCLUSIONS: The present study is one of the first to demonstrate the influence of seasonality and related abiotic factors on fish external microbiota over a year. We further identified potential interactions between gill microbiota and parasite occurrence in wild fish populations, improving current knowledge and understanding of the establishment of host-specificity.

A 2 °C difference affecting the spatiotemporal distribution of small demersal fish assemblages in shallow tropical and subtropical waters of Western Taiwan.

Two OR3 research vessel cruises were conducted at seven nearshore sites from north to south in the western coastal waters off Taiwan in May (late spring) and November (late autumn) 2019 in order to gain insights into the 2 °C difference in the tropical-subtropical fish fauna. Totally, 37 families, 72 genera, and 99 taxas were recorded for the 1809 fishes. Three fish assemblages, the North, South, and Fall-Zhuoshui River (ZRf) groups, were identified as eurythermal, stenothermal, and croaker communities. Their dominants, in rank order, were Tarphops oligolepis, Liachirus melanospilosa, Ostorhinchus fasciatus, and Trachinocephalus myops for the Northern eurythermal group, Arnoglossus tenuis, Eubleekeria splendens, and Ostorhinchus pleuron for the Southern stenothermal group, and Johnius taiwanensis, Chrysochir aureus, and Pennahia macrocephalus for the croaker-ZRf group. Their distribution was markedly correlated with the bottom water temperature, seafloor grain size, and concentration of suspended solids. The influence of a 2 °C difference on the tropical and subtropical demersal fish could indicate how rising temperatures due to climate change are shaping fish communities. The flatfish, Liachirus melanospilos, was distributed northward by 0.5oN and is suggested to be considered as an ecological indicator of the tropicalization of subtropical marine ecosystems in the future.

Depth and temperature preferences of meagre, Argyrosomus regius, as revealed by satellite telemetry.

Argyrosomus regius (commonly referred to as meagre), is one of Europe's largest coastal bony fish species and supports important recreational and commercial fisheries in the Atlantic and Mediterranean coasts. Demand for this species, and more recently for their swim bladders, has led to regional population declines and growing importance as an aquaculture species. Despite intense research in captivity, little is known about the spatial ecology of A. regius's wild population, including basic information such as vertical migrations and depth/temperature preferences. Previous research based on indirect data suggests a seasonal habitat shift from shallow to deeper waters, but this has never been validated through direct high-resolution movement data. In this study, we tagged 13 adult A. regius with pop-up satellite archival tags in the South of Portugal, which successfully returned data from 11 individuals including high-resolution data from six recovered tags (mean, range: 167 days, 28-301 days). We found that adults of this population spend 95.2% of their time between 5 and 75 m depth (mean ± SD, 30.9m ± 18.3m) and do not venture beyond 125 m. Across seasons, A. regius move across water temperatures between 13.3 and 24.8°C with a preferred thermal range between 14 and 18°C where they spent 75.4% of their time. The inferential modelling using this electronic data validated previous hypotheses by showing significant differences between a shallower and warmer summer habitat vs. a deeper and cooler winter habitat. Visual investigation of the diel effects on depth preferences suggests subtle changes in depth use between day and night during the warmer months of the year. We speculate that these patterns are in response to the species' behavioural ecology and physiology, reflecting the seasonal changes in water stratification and presence of prey, as well as on the species reproduction, which results in summer spawning aggregations in shallower areas.

Ceratocolax tavaresi n. sp. (Copepoda: Bomolochidae) parasitic in the Tomtate Grunt Haemulon aurolineatum Cuvier, 1830 (Actinopterygii: Haemulidae) off Rio de Janeiro, southeastern Brazil.

PURPOSE: A new species of the genus Ceratocolax Vervoort, 1965 is described based on specimens collected from the Tomtate grunt Haemulon aurolineatum Cuvier, caught in the coast of Angra dos Reis, off the State of Rio de Janeiro, Brazil. METHODS: One hundred specimens of H. aurolineatum were purchased from the local fish market and examined for parasitic copepods. Parasites were fixed and preserved in 80% ethanol. Morphological features of the copepods were examined and drawn using an Olympus BX51 equipped with a drawing tube. RESULTS: Ceratocolax tavaresi n. sp. can be distinguished from all congeners by the following combination of characters in the adult female: (1) second endopodal segment of leg 3 with one seta, (2) lack of stout spinules along outer margins on rami of legs 2-4, (3) genital somite without flaplike structures, (4) terminal exopodal segment of leg 4 with seven elements; and in the adult male: (1) legs 1 to 4 with 3-segmented rami (except endopod of leg 4), (2) presence of a pair of blunt processes on dorsal surface of the third pedigerous somite, (3) second endopodal segment of leg 3 with one seta. CONCLUSION: The number of species of Ceratocolax reported in the Atlantic Ocean was increased to three, including the new species. This is the forty-second species of copepod found parasitizing haemulid fish in marine waters from the Americas; however, the diversity of parasitic copepods off this continent is still underestimated.

DNA barcode reveals occurrence of threatened species and hidden diversity on Teleost fish trade in the Coastal Amazon.

This study aimed to identify the teleost fish species sold in Bragança, a major fishing hub on the north coast of Brazil. The COI gene analysis was performed for the identification of fish species. The local market uses common names that are not accurate and do not reflect the diversity of the species. 204 sequences were obtained, with 119 haplotypes. 83 species were identified by comparing with public databases and constructing phylogenetic trees, with Carangidae being the most prevalent family. The study also found Haemulon atlanticus, Menticirrhus cuiaranensis and Hoplias misioneira, a newly described species from the Amazon basin, among the samples. Additionally, 73 commercial names were recorded, including 10 categories, and the illegal trade of Epinephelus itajara was detected. The DNA Barcode method proved to be effective for discriminating the species. The study highlights that common and commercial names are vague and underestimate the fish diversity, and that Brazil needs to revise its regulations for commercial and scientific names.

Neuromolecular responses in disrupted mutualistic cleaning interactions under future environmental conditions.

BACKGROUND: Mutualistic interactions, which constitute some of the most advantageous interactions among fish species, are highly vulnerable to environmental changes. A key mutualistic interaction is the cleaning service rendered by the cleaner wrasse, Labroides dimidiatus, which involves intricate processes of social behaviour to remove ectoparasites from client fish and can be altered in near-future environmental conditions. Here, we evaluated the neuromolecular mechanisms behind the behavioural disruption of cleaning interactions in response to future environments. We subjected cleaner wrasses and surgeonfish (Acanthurus leucosternon, serving as clients) to elevated temperature (warming, 32 °C), increased levels of CO2 (high CO2, 1000 ppm), and a combined condition of elevated CO2 and temperature (warming and high CO2, 32 °C, and 1000 ppm) for 28 days. RESULTS: Each of these conditions resulted in behavioural disruptions concerning the motivation to interact and the quality of interaction (high CO2 - 80.7%, warming - 92.6%, warming and high CO2 - 79.5%, p < 0.001). Using transcriptomics of the fore-, mid-, and hindbrain, we discovered that most transcriptional reprogramming in both species under warming conditions occurred primarily in the hind- and forebrain. The associated functions under warming were linked to stress, heat shock proteins, hypoxia, and behaviour. In contrast, elevated CO2 exposure affected a range of functions associated with GABA, behaviour, visual perception, thyroid hormones and circadian rhythm. Interestingly, in the combined warming and high CO2 condition, we did not observe any expression changes of behaviour. However, we did find signs of endoplasmic reticulum stress and apoptosis, suggesting not only an additive effect of the environmental conditions but also a trade-off between physiological performance and behaviour in the cleaner wrasse. CONCLUSIONS: We show that impending environmental shifts can affect the behaviour and molecular processes that sustain mutualistic interactions between L. dimidiatus and its clients, which could have a cascading effect on their adaptation potential and possibly cause large-scale impacts on coral reef ecosystems.

Improved high-quality reference genome of red drum facilitates the processes of resistance-related gene exploration.

Sciaenops ocellatus is among the most important artificially introduced farmed fish across 11 countries and regions. However, the frequent occurrence of extreme weather events and breeding escapes have placed great pressure on local marine biodiversity and ecosystems. We reported the de novo assembly and annotation with a contig N50 of 28.30 Mb using PacBio HiFi sequencing and Hi-C technologies, which resulted in a 283-fold increase in contig N50 length and improvement in continuity and quality in complex repetitive region for S. ocellatus compared to the previous version. In total, 257.36 Mb of repetitive sequences accounted for 35.48% of the genome, and 22,845 protein-coding genes associated with a BUSCO value of 98.32%, were identified by genome annotation. Moreover, 54 hub genes rapidly responding to hypoosmotic stress were identified by WGCNA. The high-quality chromosome-scale S. ocellatus genome and candidate resistance-related gene sets will not only provide a genomic basis for genetic improvement via molecular breeding, but will also lay an important foundation for investigating the molecular regulation of rapid responses to stress.

Tracing exploitation of egg boons: an experimental study using fatty acids and stable isotopes.

Coordinated spawning of marine animals releases millions of planktonic eggs into the environment, known as egg boons. Eggs are rich in essential fatty acids and may be an important lipid subsidy to egg consumers. Our aim was to validate the application of fatty acid and stable isotope tracers of egg consumption to potential egg consumers and to confirm egg consumption by the selected species. We conducted feeding experiments with ctenophores, crustaceans and fishes. We fed these animals a common diet of Artemia or a commercial feed (Otohime) and simulated egg boons for half of them by intermittently supplementing the common diet with red drum (Sciaenops ocellatus) eggs for 10-94 days. Controls did not receive eggs. Fatty acid profiles of consumers fed eggs were significantly different from those of controls 24 h after the last egg-feeding event. Consumers took on fatty acid characteristics of eggs. In fishes and ctenophores, fatty acid markers of egg consumption did not persist 2-5 days after the last egg-feeding event, but markers of egg consumption persisted in crustaceans for at least 5-10 days. Additionally, consumption of eggs, which had high values of δ15N, led to δ15N enrichment in crustaceans and a fish. We conclude that fatty acids and nitrogen stable isotope can be used as biomarkers of recent egg consumption in marine animals, validating their use for assessing exploitation of egg boons in nature.

The evolution of NLRC3 subfamily genes in Sebastidae teleost fishes.

BACKGROUND: With more than 36,000 valid fish species, teleost fishes constitute the most species-rich vertebrate clade and exhibit extensive genetic and phenotypic variation, including diverse immune defense strategies. NLRC3 subfamily genes, which are specific to fishes, play vital roles in the immune system of teleosts. The evolution of teleosts has been impacted by several whole-genome duplication (WGD) events, which might be a key reason for the expansions of the NLRC3 subfamily, but detailed knowledge of NLRC3 subfamily evolution in the family Sebastidae is still limited. RESULTS: Phylogenetic inference of NLRC3 subfamily protein sequences were conducted to evaluate the orthology of NLRC3 subfamily genes in black rockfish (Sebastes schlegilii), 13 other fish species from the families Sebastidae, Serranidae, Gasterosteidae and Cyclopteridae, and three species of high vertebrates (bird, reptile and amphibian). WGD analyses were used to estimate expansions and contractions of the NLRC3 subfamily, and patterns of expression of NLRC3 subfamily genes in black rockfish following bacterial infections were used to investigate the functional roles of these genes in the traditional and mucosal immune system of the Sebastidae. Different patterns of gene expansions and contractions were observed in 17 fish and other species examined, and one and two whole-genome duplication events were observed in two members of family Sebastidae (black rockfish and honeycomb rockfish, Sebastes umbrosus), respectively. Subsequently, 179 copy numbers of NLRC3 genes were found in black rockfish and 166 in honeycomb rockfish. Phylogenetic analyses corroborated the conservation and evolution of NLRC3 orthologues between Sebastidae and other fish species. Finally, differential expression analyses provided evidence of the immune roles of NLRC3 genes in black rockfish during bacterial infections and gene ontology analysis also indicated other functional roles. CONCLUSIONS: We hypothesize that NLRC3 genes have evolved a variety of different functions, in addition to their role in the immune response, as a result of whole genome duplication events during teleost diversification. Importantly, this study had underscored the importance of sampling across taxonomic groups, to better understand the evolutionary patterns of the innate immunity system on which complex immunological novelties arose. Moreover, the results in this study could extend current knowledge of the plasticity of the immune system.

Anemonefishes: A model system for evolutionary genomics.

Anemonefishes are an iconic group of coral reef fish particularly known for their mutualistic relationship with sea anemones. This mutualism is especially intriguing as it likely prompted the rapid diversification of anemonefish. Understanding the genomic architecture underlying this process has indeed become one of the holy grails of evolutionary research in these fishes. Recently, anemonefishes have also been used as a model system to study the molecular basis of highly complex traits such as color patterning, social sex change, larval dispersal and life span. Extensive genomic resources including several high-quality reference genomes, a linkage map, and various genetic tools have indeed enabled the identification of genomic features controlling some of these fascinating attributes, but also provided insights into the molecular mechanisms underlying adaptive responses to changing environments. Here, we review the latest findings and new avenues of research that have led to this group of fish being regarded as a model for evolutionary genomics.

Production of Bioactive Peptides from Hake By-Catches: Optimization and Scale-Up of Enzymatic Hydrolysis Process.

Fish by-catches, along with other fish side-streams, were previously used as raw material for the production of fishmeal and fish oil but appropriate handling allows their use in more valuable options. The aim of this research was to valorize undersized hake (Merluccius merluccius) as a model of using fish by-catch from the Bay of Biscay to produce protein hydrolysates with bioactivities. Six enzymes, with different proteolytic activities (endo- or exoproteases) and specificities, were tested to produce protein hydrolysates. Products obtained with an endoprotease of serine resulted in the most promising results in terms of protein extraction yield (68%), with an average molecular weight of 2.5 kDa, and bioactivity yield (antioxidant activity = 88.5 mg TE antioxidant capacity/g fish protein; antihypertensive activity = 47% inhibition at 1 mg/mL). Then, process conditions for the use of this enzyme to produce bioactive products were optimized using Box-Behnken design. The most favorable process conditions (time = 2 h, solids = 50% and enzyme/substrate = 2% with respect to protein) were scaled up (from 0.5 L to 150 L reactor) to confirm laboratory scale and model forecasts. The results obtained in the pilot-scale testing matched the outcomes predicted by the model, confirming the technical viability of the proposed process.

Chitosan-Based Sustained Expression of Sterol Regulatory Element-Binding Protein 1a Stimulates Hepatic Glucose Oxidation and Growth in Sparus aurata.

The administration of a single dose of chitosan nanoparticles driving the expression of sterol regulatory element-binding protein 1a (SREBP1a) was recently associated with the enhanced conversion of carbohydrates into lipids. To address the effects of the long-lasting expression of SREBP1a on the growth and liver intermediary metabolism of carnivorous fish, chitosan-tripolyphosphate (TPP) nanoparticles complexed with a plasmid expressing the N terminal active domain of hamster SREBP1a (pSG5-SREBP1a) were injected intraperitoneally every 4 weeks (three doses in total) to gilthead sea bream (Sparus aurata) fed high-protein-low-carbohydrate and low-protein-high-carbohydrate diets. Following 70 days of treatment, chitosan-TPP-pSG5-SREBP1a nanoparticles led to the sustained upregulation of SREBP1a in the liver of S. aurata. Independently of the diet, SREBP1a overexpression significantly increased their weight gain, specific growth rate, and protein efficiency ratio but decreased their feed conversion ratio. In agreement with an improved conversion of dietary carbohydrates into lipids, SREBP1a expression increased serum triglycerides and cholesterol as well as hepatic glucose oxidation via glycolysis and the pentose phosphate pathway, while not affecting gluconeogenesis and transamination. Our findings support that the periodical administration of chitosan-TPP-DNA nanoparticles to overexpress SREBP1a in the liver enhanced the growth performance of S. aurata through a mechanism that enabled protein sparing by enhancing dietary carbohydrate metabolisation.