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1.
Gene ; 807: 145960, 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-34509581

RESUMO

Opsin is a fellow of the G protein-coupled receptors (GPCRs) superfamily. It can be divided into visual and non-visual opsin according to whether it is directly involved in visual imaging. Opsin plays an important role in visual image formation and the regulation of non-image forming functions such as circadian entrainment in the growth, development and evolution of fish. Crimson snapper belongs to Perciforme mainly found in the Indo-West Pacific and the South China Sea. It is one of the most influential economic fishes in the South China Sea. In order to study the existence and expression of opsin gene in Crimson snapper, we sequenced the genome and tissue sample transcriptome of Crimson snapper. In this study, 32 opsin genes were identified from the genome of Crimson snapper. The length of these genes ranged from 1061 bp to 86203 bp and were distributed on 15 different chromosomes. The analysis of opsin gene family of Crimson snapper showed that the sws2 had two extra copies as compared with that of Zebrafish. Domain and motif analysis revealed that all the 32 opsin genes have seven-(pass)-transmembrane domain receptors (7TM receptors) each, and the opsin family contained 10 common motifs. The expression level of opsin gene, confirmed by RT-qPCR, was analyzed by using nine tissues transcriptome databases of Crimson snapper. The results showed that almost all opsin genes were highly expressed in the retina and brain, except opn7a and opn7b which were expressed in intestine and red skin, and almost no expression in other tissues. Our results provide a comprehensive basic knowledge for the opsin gene family of Crimson snapper, which has significance for the study of the function of opsin in Lutjanidaes.


Assuntos
Opsinas/genética , Perciformes/genética , Animais , Sequência de Bases/genética , China , Clonagem Molecular/métodos , Doenças dos Peixes/genética , Expressão Gênica/genética , Opsinas/metabolismo , Perciformes/metabolismo , Receptores Acoplados a Proteínas G/genética , Transcriptoma/genética
2.
Zool Res ; 42(6): 746-760, 2021 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-34636194

RESUMO

Oxygen is an essential molecule for animal respiration, growth, and survival. Unlike in terrestrial environments, contamination and climate change have led to the frequent occurrence of hypoxia in aquatic environments, thus impacting aquatic animal survival. However, the adaptative mechanisms underlying fish responses to environmental hypoxia remain largely unknown. Here, we used large yellow croaker ( Larimichthys crocea) and large yellow croaker fry (LYCF) cells to investigate the roles of the Hif-1α/Hsf1/Hsp70 signaling pathway in the regulation of cellular redox homeostasis, and apoptosis. We confirmed that hypoxia induced the expression of Hif-1α, Hsf1, and Hsp70 in vivo and in vitro. Genetic Hsp70 knockdown/overexpression indicated that Hsp70 was required for maintaining redox homeostasis and resisting oxidative stress in LYCF cells under hypoxic stress. Hsp70 inhibited caspase-dependent intrinsic apoptosis by maintaining normal mitochondrial membrane potential, enhancing Bcl-2 mRNA and protein expression, inhibiting Bax and caspase3 mRNA expression, and suppressing caspase-3 and caspase-9 activation. Hsp70 suppressed caspase-independent intrinsic apoptosis by inhibiting nuclear translocation of apoptosis-inducing factor (AIF) and disturbed extrinsic apoptosis by inactivating caspase-8. Genetic knockdown/overexpression of Hif-1α and dual-luciferase reporter assay indicated that Hif-1α activated the Hsf1 DNA promoter and enhanced Hsf1 mRNA transcription. Hsf1 enhanced Hsp70 mRNA transcription in a similar manner. In summary, the Hif-1α/Hsf1/Hsp70 signaling pathway plays an important role in regulating redox homeostasis and anti-apoptosis in L. crocea under hypoxic stress.


Assuntos
Fatores de Transcrição de Choque Térmico/metabolismo , Homeostase/fisiologia , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Oxigênio/farmacologia , Perciformes/metabolismo , Transdução de Sinais/fisiologia , Animais , Apoptose , Linhagem Celular , Clonagem Molecular , Biologia Computacional , Regulação da Expressão Gênica/efeitos dos fármacos , Fatores de Transcrição de Choque Térmico/genética , Homeostase/efeitos dos fármacos , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Oxirredução , Oxigênio/química , Perciformes/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Água/química
3.
Mar Biotechnol (NY) ; 23(5): 749-765, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34647196

RESUMO

We investigated a time-course larval transcriptional analysis (RNA-seq) in the longfin yellowtail Seriola rivoliana, from hatching to day four at 22 °C, without providing zooplankton as food. Larval starvation is a critical physiological stage that must be prevented to ensure survival. However, the transcriptional mechanisms to endure starvation have not been investigated in marine fish. Differential gene expression showed newly day-specific transcriptome events during larval development. On day 1 (yolk sac absorption), the predominant upregulated developmental processes were larval growth, muscle and vision development, cytoskeletal structure, protein synthesis, protein and fat digestion-absorption, and hormone biosynthesis, whereas the cell cycle was suppressed. On day 2 (yolk sac exhaustion), a new stage of energy regeneration (ATP) was supplied by the oil drop reserve, whereas protein digestion-absorption and growth were suppressed. On day 3 (mouth opening and starvation), stress signals and nutrition deprivation upregulated the p53 signal and triggered autophagy and the AMP-activated protein kinase (AMPK) pathways as an alternative catabolic pathway to enduring starvation, and the circadian rhythm was established. On day 4 (starving and weakened larvae condition), autophagy supported subsequent protein synthesis, activated the immune system, and promoted estrogen signaling and skeleton renovation. However, larvae suppressed muscle development, vision and carbohydrate, and fat digestion-absorption and became lethargic, evidencing limited physiological support by autophagy to maintain survival without exogenous nutrition in this species.


Assuntos
Privação de Alimentos , Perciformes/crescimento & desenvolvimento , Perciformes/metabolismo , Animais , Autofagia , Larva/genética , Larva/crescimento & desenvolvimento , Larva/metabolismo , Perciformes/genética , Transcriptoma
4.
Molecules ; 26(20)2021 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-34684776

RESUMO

Oxidative stress in aquatic organisms might suppress the immune system and propagate infectious diseases. This study aimed to investigate the protective effect of polyphenolic extracts from spent coffee grounds (SCG) against oxidative stress, induced by H2O2, in C. viridis brain cells, through an in vitro model. Hydrophilic extracts from SCG are rich in quinic, ferulic and caffeic acids and showed antioxidant capacity in DPPH, ORAC and FRAP assays. Furthermore, pretreatment of C. viridis brain cells with the polyphenolic extracts from SCG (230 and 460 µg/mL) for 24 h prior to 100 µM H2O2 exposure (1 h) significantly increased antioxidant enzymes activity (superoxide dismutase and catalase) and reduced lipid peroxidation (measured by MDA levels). These results suggest that polyphenols found in SCG extracts exert an antioxidative protective effect against oxidative stress in C. viridis brain cells by stimulating the activity of SOD and CAT.


Assuntos
Antioxidantes/química , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Café/química , Perciformes/metabolismo , Animais , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Encéfalo/citologia , Catalase/metabolismo , Células Cultivadas , Coffea/química , Proteínas de Peixes/metabolismo , Pesqueiros , Sequestradores de Radicais Livres/química , Sequestradores de Radicais Livres/isolamento & purificação , Sequestradores de Radicais Livres/farmacologia , Peróxido de Hidrogênio/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Polifenóis , Superóxido Dismutase/metabolismo
5.
FASEB J ; 35(10): e21900, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34547130

RESUMO

Previous studies have shown that endoplasmic reticulum (ER) stress contributes to hepatic steatosis in several manners. However, how lipid droplet (LD) proteins participate in this process has rarely been reported. In the present study, ER stress was induced at both in vitro and in vivo levels with tunicamycin in large yellow croaker (Larimichthys crocea). Effects of LD protein perilipin2 (PLIN2) on hepatic lipid accumulation and lipoprotein transport under normal physiological condition and ER stress were then explored using dsRNA mediated knockdown. Subsequently, the transcriptional regulation of plin2 expression by transcription factors generated in the unfolded protein response (UPR) was determined by dual-luciferase reporter assays, chromatin immunoprecipitation and electrophoretic mobility-shift assay. We demonstrated that ER stress could promote LDs accumulation and inhibit lipoprotein transport by transcriptionally upregulating PLIN2 in liver. Among the transcription factors generated by UPR, spliced X-box binding protein1 can directly upregulated the expression of plin2, whereas C/EBP homologous protein can upregulate the expression of plin2 through peroxisome proliferator activated-receptor α. These results revealed that the LD protein PLIN2 played an important role in ER stress-induced hepatic steatosis, which might be a novel mechanism explaining hepatic steatosis triggered by ER stress.


Assuntos
Estresse do Retículo Endoplasmático , Fígado Gorduroso/metabolismo , Proteínas de Peixes/biossíntese , Perciformes/metabolismo , Perilipina-2/biossíntese , Transcrição Genética , Regulação para Cima , Animais
6.
J Fish Dis ; 44(12): 2111-2123, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34585397

RESUMO

Amyloodinium ocellatum (AO) infection in silver pomfret (Pampus argenteus) causes extensive mortality. Insufficient information exists on the molecular immune response of silver pomfret to AO infestation, so herein we simulated the process of silver pomfret being infected by AO. Translucent trophosomes were observed on the gills of AO-infected fish. Transcriptome profiling was performed to investigate the effects of AO infection on the gill, kidney complex and spleen. Overall, 404,412,298 clean reads were obtained, assembling into 96,341 unigenes, which were annotated against public databases. In total, 2730 differentially expressed genes were detected, and few energy- and immune-related genes were further assessed using RT-qPCR. Moreover, activities of three immune-related (SOD, AKP and ACP) and three energy-related (PKM, LDH and GCK) enzymes were determined. AO infection activated the immune system and increased interleukin-1 beta and immunoglobulin M heavy chain levels. Besides, the PPAR signalling pathway was highly enriched, which played a role in improving immunity and maintaining homeostasis. AO infection also caused dyspnoea, leading to extensive lactic acid accumulation, potentially contributing towards a strong immune response in the host. Our data improved our understanding regarding the immune response mechanisms through which fish coped with parasitic infections and may help prevent high fish mortality in aquaculture.


Assuntos
Dinoflagelados/fisiologia , Doenças dos Peixes/imunologia , Perciformes/imunologia , Infecções Protozoárias em Animais/imunologia , Animais , Dinoflagelados/patogenicidade , Dispneia/veterinária , Doenças dos Peixes/parasitologia , Perfilação da Expressão Gênica , Brânquias/parasitologia , Perciformes/genética , Perciformes/metabolismo
7.
Vet Parasitol ; 298: 109533, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34411977

RESUMO

The parasite Cryptocaryon irritans causes massive losses in the marine fish culture industry and is one of the most threatening pathogens affecting teleost species. The acute death of infected fish is primarily caused by the destruction of gill cells, resulting in osmotic imbalance and respiratory stress. C. irritans has wide host specificity; however, the yellow drum Nibea albiflora is highly resistant to this parasite. Metabolomic approaches in combination with transcriptomic analysis were used to characterize the host immune reaction and metabolic changes in yellow drum in response to C. irritans infection and to identify the key genes and compounds in the gills that have the strongest contribution to disease resistance. The yellow drum was challenged with theronts at a median death rate (2050 theronts per gram fish). The samples were collected from the gills 24 h and 72 h after the infection (hpi). The results of metabolomic analysis indicated that metabolites involved in energy metabolism were predominantly downregulated. In contrast, a compensatory increase in the expression of the genes involved in the citric acid cycle and glycolysis was detected 24 hpi. The suppression of metabolites was alleviated after feed intake recovery 72 hpi. The levels of amino acids were decreased, and the expression of aminoacyl-tRNA was increased. Additionally, elevated levels of arachidonic acid derivatives, primarily prostaglandins, were responsible for anti-inflammatory, osmotic, and hypoxia regulations. Purine metabolism was also involved in the immune response via generation of reactive oxygen species catalyzed by xanthine oxidase. A significant increase in the generation of retinoic acid, which could enhance mucosal adaptive immunity by stimulating the synthesis of antibodies and accelerating the restoration of epithelial integrity, was observed at 72 hpi. This result was consistent with high expression of the genes related to secreted immunoglobulin T 72 hpi. In conclusion, the present study comprehensively described the key compounds and genes related to C. irritans infection in yellow drum gills. Biomarkers that were significantly changed during the infection may represent future targets for nutritional intervention to enhance host immunity against C. irritans infection and to accelerate disease recovery.


Assuntos
Infecções por Cilióforos , Doenças dos Peixes , Brânquias , Metaboloma , Perciformes , Transcriptoma , Animais , Cilióforos , Infecções por Cilióforos/veterinária , Doenças dos Peixes/metabolismo , Doenças dos Peixes/parasitologia , Brânquias/metabolismo , Brânquias/parasitologia , Perciformes/metabolismo , Perciformes/parasitologia
8.
Chem Pharm Bull (Tokyo) ; 69(9): 918-925, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34234056

RESUMO

Yellow Croaker Ear-stone or Yunaoshi, is actually two kinds of fish otolith in China and has received increased attention in recent years as important folk medicine. For better understanding of this crude drug, a chaotic market circulation status investigation was carried out and seventeen samples with different varieties or producing areas were collected. In this study, pharmacodynamic components of nineteen varieties mineral elements of the seventeen samples were simultaneously determined by Inductively coupled plasma mass spectrometry (ICP-MS) method. The detected elements were categorized into the beneficial (Na, Mg, Ca, K, Fe, Mn, Zn, Sr, B) and unbeneficial elements (Cu, As, Cd, Hg, Al, Pb, Co, Ba, Cr and Ni) kinds and their concentrations were quantified. Then the principal component analysis (PCA) and hierarchical clustering analysis (HCA) were further applied to launch an exploratory analysis for Yunaoshi samples. The results showed that samples 1-3, 15-8, 15-3 ranked the top three from the perspective of beneficial elements and samples 1-3, 1-4, 15-2 ranked the top three based on the unbeneficial elements sides. Combined with HCA results, all samples can be used as the substitutes for Yunaoshi except for samples 1-3, 1-4 and 15-2 only judging from the perspective of mineral elements concentrations. In conclusion, simultaneous determination of mineral elements accompanied with PCA and HCA can not only provide pharmacogenetic reference for the medicinal material of Yunaoshi, but also establish a feasibility for exploring new crude resources or substitutes to this medicine.


Assuntos
Espectrometria de Massas , Minerais/análise , Perciformes/metabolismo , Animais , China , Análise por Conglomerados , Medicina Tradicional Chinesa , Análise de Componente Principal
9.
Artigo em Inglês | MEDLINE | ID: mdl-34174451

RESUMO

Embryonic and early larval development and metabolism of most teleost fishes are fueled entirely by maternally derived nutritional resources (yolk and oil) before the onset of exogenous feeding. The composition of those resources depends, in part, on maternal diet. To examine how diet-induced variations in egg composition affect embryonic and larval utilization of endogenous lipids and fatty acids, we fed red drum (Sciaenops ocellatus) broodstock four different diets to produce distinctive fatty acid compositions in eggs but with no significant difference in total lipid content. We sampled embryos and unfed larvae every 12 h from 12 h post-fertilization (hpf) until starvation (96-120 hpf; n = 5 spawns per diet group). Rates of utilization of the oil globule were significantly different among diet groups, resulting in significant differences in mean oil globule size at the first feeding stage (84 hpf). Utilization rates for 15 fatty acids were significantly different among diet groups and were proportional to their initial concentration. As a result, differences in larval fatty acid compositions among maternal diet groups diminished over time but remained different even at later stages. In addition, larval standard length at 84 hpf was positively correlated with egg total lipid content. This study suggests that the fatty acid composition of yolk and oil affects energy metabolism and tissue composition in red drum larvae. These effects could have consequences for the survival and essential physiological functions of fish larvae during the critical period of transition to exogenous feeding.


Assuntos
Dieta/veterinária , Embrião não Mamífero/citologia , Larva/crescimento & desenvolvimento , Lipídeos/análise , Exposição Materna/estatística & dados numéricos , Fenômenos Fisiológicos da Nutrição Materna , Perciformes/crescimento & desenvolvimento , Animais , Embrião não Mamífero/metabolismo , Feminino , Larva/metabolismo , Perciformes/metabolismo
10.
Biomolecules ; 11(5)2021 05 09.
Artigo em Inglês | MEDLINE | ID: mdl-34065058

RESUMO

New data on lipid and fatty acid profiles are presented, and the dynamics of the studied components in muscles in the males and females of the beaked redfish, Sebastes mentella, in the depth gradient of the Irminger Sea (North Atlantic) is discussed. The contents of the total lipids (TLs), total phospholipids (PLs), monoacylglycerols (MAGs), diacylglycerols (DAGs), triacylglycerols (TAGs), cholesterol (Chol), Chol esters, non-esterified fatty acids (NEFAs), and wax esters were determined by HPTLC; the phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine (PC), and lysophosphatidylcholine (LPC) were determined by HPLC; and fatty acids of total lipids were determined using GC. The Chol esters prevailed in muscles over the storage TAGs, and the wax ester content was high, which is a characteristic trait of vertically migrating species. Specific dynamics in certain PL in redfish were found to be depended on depth, suggesting that PLs are involved in the re-arrangement of the membrane physicochemical state and the maintenance of motor activity under high hydrostatic pressure. The high contents of DHA and EPA were observed in beaked redfish muscles is the species' characteristic trait. The MUFAs in muscles include dietary markers of zooplankton (copepods)-20:1(n-9) and 22:1(n-11), whose content was found to be lower in fish sampled from greater depths.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Ácidos Graxos/análise , Lipídeos/análise , Músculos/metabolismo , Perciformes/metabolismo , Água do Mar/química , Animais
11.
Mar Pollut Bull ; 170: 112661, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34182302

RESUMO

There are many toxicological studies on microplastics, but little is known about the effect of nanoplastics (NPs). Here, we evaluated the oxidative stress responses induced by NPs (10, 104 and 106 particles/l) in juvenile Larimichthys crocea during 14-d NPs exposure followed by a 7-d recovery. After exposure, the activities of antioxidant enzymes (SOD, CAT, GPx) and MDA levels increased in the liver of fish at the highest NPs concentration. SOD and CAT activities remained elevated above the baseline after recovery under high-concentration NPs but returned to the baseline in two other NP treatments. Although lipid peroxidation in liver was reversible, juvenile fish in NPs treatments exhibited a lower survival rate than the control during both exposure and recovery. Furthermore, IBR value and PCA analysis showed the potential adverse effects of NPs. Considering that NPs can reduce the survival of fish juveniles, impacts of NPs on fishery productivity should be considered.


Assuntos
Microplásticos , Perciformes , Animais , Proteínas de Peixes/metabolismo , Fígado/metabolismo , Estresse Oxidativo , Perciformes/metabolismo , Plásticos/metabolismo
12.
Free Radic Biol Med ; 172: 213-225, 2021 08 20.
Artigo em Inglês | MEDLINE | ID: mdl-34116177

RESUMO

Lipid overload-induced hepatic steatosis is a major public health problem worldwide. However, the potential molecular mechanism is not completely understood. Herein, we found that high-fat diet (HFD) or oleic acid (OA) treatment induced oxidative stress which prevented the entry of hepatocyte nuclear factor 4 alpha (HNF4α) into the nucleus by activating protein kinase C delta (PKCδ) in vivo and in vitro in large yellow croaker (Larimichthys crocea). This reduced the level of microsomal triglyceride transfer protein (MTP) transcription, resulting in the impaired secretion of very-low-density lipoprotein (VLDL) and the abnormal accumulation of triglyceride (TG) in hepatocytes. Meanwhile, the detrimental effects induced by lipid overload could be partly alleviated by pretreating hepatocytes with Go6983 (PKCδ inhibitor) or N-acetylcysteine (NAC, reactive oxygen species (ROS) scavenger). In conclusion, for the first time, we revealed that lipid overload impaired hepatic VLDL secretion via oxidative stress-mediated PKCδ-HNF4α-MTP pathway in fish. This study may provide critical insights into potential intervention strategies against lipid overload-induced hepatic steatosis of fish and human beings.


Assuntos
Lipoproteínas VLDL , Perciformes , Animais , Proteínas de Transporte , Hepatócitos/metabolismo , Humanos , Lipoproteínas VLDL/metabolismo , Fígado/metabolismo , Estresse Oxidativo , Perciformes/metabolismo
13.
Mar Biotechnol (NY) ; 23(3): 445-455, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33993358

RESUMO

Ocean acidification is changing the fate of marine organisms. It is essential to predict the biological responses and evolutionary processes driven by ocean acidification, to maintain the equilibrium of the marine ecosystem and to facilitate aquaculture. However, how marine organisms, particularly the marine fish species, respond to ocean acidification, is still poorly understood. Consequences of ocean acidification on finfish aquaculture are largely not well known. We studied the effects of ocean acidification for 7 days on growth, behaviour and gene expression profiles in the brain, gill and kidney of Asian seabass juveniles. Results showed that growth and behaviour were not affected by short-term ocean acidification. We found tissue-specific differentially expressed genes (DEGs) involving many molecular processes, such as organ development, growth, muscle development, ion homeostasis and neurogenesis and development, as well as behaviours. Most of the DEGs, which were functionally enriched in ion homeostasis, were related to calcium transport, followed by sodium/potassium channels. We found that genes associated with neurogenesis and development were significantly enriched, implying that ocean acidification has also adversely affected the neural regulatory mechanism. Our results indicate that although the short-term ocean acidification does not cause obvious phenotypic and behavioural changes, it causes substantial changes of gene expressions in all three analysed tissues. All these changes of gene expressions may eventually affect physiological fitness. The DEGs identified here should be further investigated to discover DNA markers associated with adaptability to ocean acidification to improve fish's capability to adapt to ocean acidification.


Assuntos
Perciformes/crescimento & desenvolvimento , Água do Mar/química , Transcriptoma , Animais , Aquicultura , Comportamento Animal , Encéfalo/metabolismo , Dióxido de Carbono , Brânquias/metabolismo , Concentração de Íons de Hidrogênio , Rim/metabolismo , Perciformes/genética , Perciformes/metabolismo
14.
Food Funct ; 12(11): 4825-4841, 2021 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-33949580

RESUMO

Large yellow croaker roe phospholipids (LYCRPLs) have great nutritional value because they are rich in docosahexaenoic acid (DHA), which is an n-3 polyunsaturated fatty acid (n-3 PUFA). In previous research, we studied the effect of LYCRPLs on the inhibition of triglyceride accumulation at the cellular level. However, its lipid regulation effect in rats on a high-fat diet and its influence on the gut microbiota has not yet been clarified. In this study, a high-fat diet was used to induce the lipid metabolism disorder in SD rats, and simvastatin, low-dose, medium-dose and high-dose LYCRPLs were given by intragastric administration for 8 weeks. The rats' body weight, food intake, organ index, blood biochemical indicators, epididymal fat tissue and liver histopathology were compared and analyzed. High-throughput 16S rRNA gene sequencing technology and bioinformatics analysis technology were also used to analyze the diversity of gut microbiota in rats. We found that LYCRPLs can significantly regulate lipid metabolism, and improve the gut microbiota disorder induced in rats by a high-fat diet. These results can lay a foundation for the study of the regulation mechanism of LYCRPLs lipid metabolism, and also provide a theoretical basis for the development of LYCRPLs as functional food additives and excipients with hypolipidemic effects.


Assuntos
Dieta Hiperlipídica/efeitos adversos , Ácidos Docosa-Hexaenoicos/farmacologia , Microbioma Gastrointestinal/efeitos dos fármacos , Transtornos do Metabolismo dos Lipídeos/tratamento farmacológico , Perciformes/metabolismo , Fosfolipídeos/farmacologia , Animais , Peso Corporal , Aditivos Alimentares/metabolismo , Alimento Funcional , Metabolismo dos Lipídeos/efeitos dos fármacos , Transtornos do Metabolismo dos Lipídeos/induzido quimicamente , Fígado/metabolismo , Masculino , RNA Ribossômico 16S/genética , Ratos , Ratos Sprague-Dawley , Triglicerídeos/metabolismo
15.
Am Nat ; 197(5): E156-E172, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33908823

RESUMO

AbstractIt is well accepted that the complexity of functional systems may mitigate performance trade-offs. However, data supporting this theory are hard to find because they need to be based on a functional system with different complexity levels in closely related species. The Pomacentridae (damselfishes) provide an excellent opportunity to test this hypothesis because most of the species have two mouth-closing systems: the first using the adductor mandibulae, as in all teleost fishes, and the second relying on the ceratomandibular (cmd) ligament, a synapomorphic trait of the family. Interestingly, some pomacentrids have secondarily lost the cmd ligament during evolution and therefore have a less complex mouth-closing system. Using dissection, kinematic analysis, and mathematical modeling, we demonstrated that the possession of two mouth-closing systems enabled grazing damselfishes to have a forceful and extremely fast bite. This combination challenges a major functional trade-off in fish jaw dynamics, as systems better suited for force transmission are usually less suited for speed transmission, and vice versa. The combination of grazing behavior, small and robust lower jaws (conferring high biting force), and an ultrafast bite is unusual within actinopterygians. These attributes and their associated performance seem to be required conditions to colonize the ecological niche of farming, that is, the maintenance of small filamentous algae crops serving as both food and storage.


Assuntos
Comportamento Alimentar , Herbivoria , Perciformes , Animais , Ecossistema , Comportamento Alimentar/fisiologia , Arcada Osseodentária/anatomia & histologia , Modelos Biológicos , Perciformes/anatomia & histologia , Perciformes/metabolismo
16.
Toxins (Basel) ; 13(3)2021 03 17.
Artigo em Inglês | MEDLINE | ID: mdl-33803043

RESUMO

Ciguatera fish poisoning (CFP) is one of the most frequently encountered seafood poisoning syndromes; it is caused by the consumption of marine finfish contaminated with ciguatoxins (CTXs). The majority of CFP cases result from eating fish flesh, but a traditional belief exists among people that the head and viscera are more toxic and should be avoided. Unlike the viscera, scientific data to support the legendary high toxicity of the head is scarce. We prepared tissue samples from the fillet, head, and eyes taken from five yellow-edged lyretail (Variola louti) individuals sourced from Okinawa, Japan, and analyzed the CTXs by LC-MS/MS. Three CTXs, namely, CTX1B, 52-epi-54-deoxyCTX1B, and 54-deoxyCTX1B, were confirmed in similar proportions. The toxins were distributed nearly evenly in the flesh, prepared separately from the fillet and head. Within the same individual specimen, the flesh in the fillet and the flesh from the head, tested separately, had the same level and composition of toxins. We, therefore, conclude that flesh samples for LC-MS/MS analysis can be taken from any part of the body. However, the tissue surrounding the eyeball displayed CTX levels two to four times higher than those of the flesh. The present study is the first to provide scientific data demonstrating the high toxicity of the eyes.


Assuntos
Intoxicação por Ciguatera , Ciguatoxinas/análise , Contaminação de Alimentos/análise , Perciformes/metabolismo , Alimentos Marinhos/análise , Animais , Carga Corporal (Radioterapia) , Cromatografia Líquida , Olho/metabolismo , Cabeça , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas em Tandem , Distribuição Tecidual
17.
Sci Rep ; 11(1): 6230, 2021 03 18.
Artigo em Inglês | MEDLINE | ID: mdl-33737614

RESUMO

The purpose of subject was to explore the optimum protein requirement of juvenile grouper (Epinephelus coioides). In the test, 450 juveniles with an average weight (10.02 ± 0.22) g were randomly divided into six groups with triplicate, and were fed with 350, 400, 450, 500, 550 and 600 g/kg iso-lipid test diet twice 1 day for 8 weeks, respectively. The results showed that: (1) With the increase of protein level, the body weight gain rate and specific growth rate first increased and then reduced, while the feed coefficient rate first decreased and then increased, while the protein efficiency significantly decreased (P < 0.05). (2) With the increase of protein level, the condition factor, hepaticsomatic index and visceralsomatic index significantly reduced (P < 0.05). (3) With the increase of protein level, the crude protein content of whole fish and muscle gradually increased, while the crude lipid content gradually decreased. (4) High-protein diet (550-600 g/kg) significantly increased the plasma total protein content and decreased the triglyceride content of orange-spotted grouper (P < 0.05). (5) Compared with the 350 g/kg group, 500, 550, 600 g/kg groups significantly increased the activities of glutamic-pyruvic transaminase and glutamic oxaloacetic transaminase in liver (P < 0.05). (6) With the increase of protein level, the protease activity of intestine first increased and then decreased, and reached the maximum at the protein level of 500 g/kg, while lipase and amylase decreased significantly (P < 0.05). (7) The activities of acid phosphatase, superoxide dismutase and lysozyme in liver increased first and then decreased with the increase of protein level, and reached the maximum in the 400 g/kg protein group. According to the analysis specific growth rate, the optimum protein level of juvenile orange-spotted grouper is 521.84 g/kg.


Assuntos
Ração Animal/análise , Proteínas na Dieta/administração & dosagem , Proteínas de Peixes/genética , Intestinos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Perciformes/metabolismo , Fosfatase Ácida/genética , Fosfatase Ácida/metabolismo , Tecido Adiposo/efeitos dos fármacos , Tecido Adiposo/crescimento & desenvolvimento , Alanina Transaminase/genética , Alanina Transaminase/metabolismo , Amilases/genética , Amilases/metabolismo , Animais , Aspartato Aminotransferases/genética , Aspartato Aminotransferases/metabolismo , Composição Corporal/efeitos dos fármacos , Composição Corporal/fisiologia , Proteínas de Peixes/metabolismo , Expressão Gênica , Intestinos/crescimento & desenvolvimento , Lipase/genética , Lipase/metabolismo , Fígado/metabolismo , Muramidase/genética , Muramidase/metabolismo , Músculos/efeitos dos fármacos , Músculos/fisiologia , Peptídeo Hidrolases/genética , Peptídeo Hidrolases/metabolismo , Perciformes/genética , Perciformes/crescimento & desenvolvimento , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismo , Triglicerídeos/metabolismo , Ganho de Peso/efeitos dos fármacos , Ganho de Peso/fisiologia
18.
Fish Physiol Biochem ; 47(4): 811-828, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33694040

RESUMO

In order to study the variation of gonad lipidomics during reproductive cycle, black rockfish was employed as the research model in the present study. Using histology, lipidomics, and qPCR, the profile of gonad lipidomics and the expression levels of related genes during different developmental stages were detected and analyzed to show the potential regulatory network of lipid metabolism. Based on Ultra High-Performance Liquid Tandem Chromatography Quadrupole Time of Flight Mass Spectrometry (UHPLC-QTOFMS), four significant differential glycerophospholipid metabolic pathways including phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), and phosphatidic acid (PA) were enriched by KEGG. Pathway-related enzyme-coding genes, including phosphatidylserine decarboxylase (pisd), phosphatidylserine synthase (ptdss1, ptdss2), and phospholipase D (pld1, pld2) were identified from the whole genome data and confirmed by cloning. The expression profiles of these genes were tested by qPCR in the tissues and gonads in developmental stages, and we found that pisd, pld, and ptdss genes were all downregulated through the developmental process in the brain of male, and the latter two genes were upregulated in the liver and testis at stage IV, which were the opposite trend observed in the female. Thus, our findings would be helpful in further understanding the substance metabolism and regulation during gonad development in ovoviviparity teleosts.


Assuntos
Glicerofosfolipídeos/metabolismo , Ovário/crescimento & desenvolvimento , Ovário/metabolismo , Perciformes , Testículo/crescimento & desenvolvimento , Testículo/metabolismo , Animais , Feminino , Expressão Gênica , Lipidômica , Masculino , Ovoviviparidade/genética , Perciformes/genética , Perciformes/crescimento & desenvolvimento , Perciformes/metabolismo , Filogenia
19.
J Biol Chem ; 296: 100414, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33581111

RESUMO

Increasing evidence shows that the long noncoding RNA (lncRNA) is a major regulator and participates in the regulation of various physiological and pathological processes, such as cell proliferation, differentiation, metastasis, and apoptosis. Unlike mammals, however, the study of lncRNA in lower invertebrates is just beginning and the extent of lncRNA-mediate regulation remains unclear. Here, we for the first time identify an lncRNA, termed nucleotide oligomerization domain 1 (NOD1) antibacterial and antiviral-related lncRNA (NARL), as a key regulator for innate immunity in teleost fish. We found that NOD1 plays an important role in the antibacterial and antiviral process in fish and that the microRNA miR-217-5p inhibits NOD1 expression and thus weakens the NF-κB and the IRF3-driven signaling pathway. Furthermore, our results indicated that NARL functions as a competing endogenous RNA (ceRNA) for miR-217-5p to regulate protein abundance of NOD1; thus, invading microorganisms are eliminated and immune responses are promoted. Our study also demonstrates the regulation mechanism that lncRNA NARL can competitive adsorption miR-217-5p to regulate the miR-217-5p/NOD1 axis is widespread in teleost fish. Taken together, our results reveal that NARL in fish is a critical positive regulator of innate immune responses to viral and bacterial infection by suppressing a feedback to NOD1-NF-κB/IRF3-mediated signaling.


Assuntos
Imunidade Inata/genética , Perciformes/imunologia , Animais , Apoptose , Proliferação de Células , China , Expressão Gênica , Regulação da Expressão Gênica/genética , Fator Regulador 3 de Interferon/metabolismo , MicroRNAs/genética , NF-kappa B/metabolismo , Proteína Adaptadora de Sinalização NOD1/genética , Proteína Adaptadora de Sinalização NOD1/metabolismo , Perciformes/genética , Perciformes/metabolismo , RNA Longo não Codificante/genética , Transdução de Sinais
20.
Fish Physiol Biochem ; 47(2): 393-407, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33547601

RESUMO

Meiosis is a specialized type of cell division critical for gamete production during sexual reproduction in eukaryotes. The meiotic recombination protein Rec8 has been identified as an important factor in germ cell meiotic initiation in vertebrates; however, its equivalent role in teleosts is poorly characterized. In this study, we cloned and sequenced the rec8 gene from orange-spotted grouper (Epinephelus coioides). The cDNA sequence consisted of 2244 base pairs (bp), including a 5' untranslated region (UTR) of 198 bp and a 3'UTR of 284 bp. The open reading frame of grouper rec8 was 1752 bp, encoding 584 amino acids. Expression levels of rec8 were higher in the ovary, intersex gonad, and testis. A neighbor-joining phylogenetic tree based on the deduced amino acid sequence indicated a common origin for grouper and other teleost rec8 molecules. Immunohistochemistry using a polyclonal anti-Rec8 antibody localized the protein in the oogonia and primary oocytes in the ovary and in spermatogonia and spermatocytes in the intersex gonad and testis, suggesting that Rec8 may play an important role in the meiotic division and the development of grouper germ cells. In addition, we found that the transcription factor Dmrt1 increased rec8 promoter activity through the second binding site, based on dual-luciferase assays. Together, these results suggest that Rec8 plays a crucial role in meiosis and may be regulated by Dmrt1 to affect meiosis in groupers.


Assuntos
Proteínas de Ciclo Celular/metabolismo , Clonagem Molecular , Perciformes/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Proteínas de Ciclo Celular/genética , Feminino , Masculino , Perciformes/genética , Filogenia , Transporte Proteico
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