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1.
Cornea ; 38(8): 951-954, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31276458

RESUMO

PURPOSE: To describe the outcomes of tuck-in tenon patch graft (TPG) in the management of corneal perforation up to 5-mm size. METHODS: Thirty-one cases of sterile corneal perforation (3-5 mm) underwent autologous TPG. The technique included, freshening of the edges, measuring the size of defect, creating a 360-degree stromal pocket around the perforation margin, harvesting the tenon graft followed by tucking into the stromal pocket, and application of cyanoacrylate glue or suturing the graft using 10-0 monofilament nylon suture. The main outcome measure was rate of healing (percentage of cases healed). RESULTS: The mean age was 52.3 ± 8.9 years with 22 male and 9 female patients. The various etiologies of corneal perforation included trauma (n = 10), neurotrophic keratitis (n = 11), and peripheral ulcerative keratitis (n = 10). The mean size of corneal perforation was 4.2 ± 0.6 mm (range 3-5 mm). The mean duration of epithelial healing was 25.7 ± 6.7 days. Best-corrected visual acuity improved from 1.8 ± 0.4 to 1.2 ± 0.4 logarithm of the minimum angle of resolution units at 4 weeks after surgery (P ≤ 0.01). Twenty-seven (87.1%) cases healed with formation of a leucomatous scar at 16.9 ± 2.7 weeks, whereas 4 cases had a flat anterior chamber. In three-fourths of the cases, a corneal graft was performed. In one case, graft resuturing was performed for post-op aqueous leak, which healed with the formation of a corneo-iridic scar. CONCLUSIONS: TPG is a safe, simple, inexpensive, and an effective technique for the management of corneal perforations. The advantages include the autologous nature of the graft, cost effectiveness, and easy availability.


Assuntos
Perfuração da Córnea/cirurgia , Úlcera da Córnea/cirurgia , Cápsula de Tenon/transplante , Adulto , Idoso , Perfuração da Córnea/fisiopatologia , Úlcera da Córnea/fisiopatologia , Cianoacrilatos/uso terapêutico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Técnicas de Sutura , Adesivos Teciduais/uso terapêutico , Transplante Autólogo , Acuidade Visual/fisiologia , Cicatrização/fisiologia
2.
Chin Med J (Engl) ; 129(15): 1817-21, 2016 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-27453231

RESUMO

BACKGROUND: Small incision refractive lenticule extraction (SMILE) is an effective laser procedure that treats myopia. This research was to describe a novel approach to treat corneal ulcer or perforation using the corneal lenticules obtained from SMILE and to evaluate the safety and effectiveness of tectonic keratoplasty with femtosecond laser intrastromal lenticule (TEKIL). METHODS: A total of twenty patients (22 eyes) were monitored for at least 6 months and were assessed using slit lamp microscopy, optical coherence tomography, and best-corrected visual acuity (BCVA). Postoperative complications throughout the study period were recorded. RESULTS: Corneal ulcer in 14 patients (16 eyes) and corneal perforation in six patients (6 eyes) were treated with TEKIL. The patients were ten females and ten males, with a mean age of 58.5 ± 16.3 years (range: 16-81 years). In this study, the most causes of corneal ulcer or perforation were immunologic causes (54.5%). After TEKIL procedure, global integrity was achieved in all cases. No immune rejection or perforation was detected. The mean BCVA improved from 0.17 ± 0.20 preoperatively to 0.27 ± 0.25 postoperatively at the final follow-up (t = 2.095, P = 0.052). The postoperative BCVA improved in 12 eyes (54.5%) and maintained in nine eyes (40.9%). Vision function successfully maintained in all eyes (100%). In three cases, corneal ulcers were treated by reoperation of TEKIL at 3 months after the initial surgery for the reason that the residual corneal thickness was <250 µm. CONCLUSIONS: TEKIL seems to be an effective treatment for corneal ulcer and perforation in the condition of emergency and donor shortage.


Assuntos
Córnea/cirurgia , Perfuração da Córnea/cirurgia , Transplante de Córnea/métodos , Úlcera da Córnea/cirurgia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Perfuração da Córnea/fisiopatologia , Transplante de Córnea/efeitos adversos , Úlcera da Córnea/fisiopatologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias , Estudos Retrospectivos , Tomografia de Coerência Óptica , Acuidade Visual/fisiologia , Adulto Jovem
3.
Vestn Oftalmol ; 132(2): 70-72, 2016 Mar-Apr.
Artigo em Russo | MEDLINE | ID: mdl-27213800

RESUMO

The paper presents an original method for temporary biokeratoprothetics in total destruction of the cornea, which consists of the following. In the operating room a 12-mm disc is cut out of patient's conchal cartilage by a trephine and then thinned down to 1 mm with a blade. The prepared autograft is placed in front of the iris completely overlapping corneal defect and sutured to the sclera with 10--12 U-shaped interrupted stitches using a 7/0 suture. Between the stitches 0.2--0.3 ml of viscoelastic are injected into the anterior chamber. Temporary blepharorrhaphy is done within the temporal one-third of the eyelids with a U-shaped suture 6/0. The authors present an own clinical observation of patient D., aged 46, with purulent corneal ulcer and total destruction of the cornea. In ophthalmic emergency, if no donor cornea is available, the described method allows to save the eyeball from its anatomical and functional loss. Further routine keratoplasty or optical keratoprosthetics may bring some functional improvement.


Assuntos
Perfuração da Córnea , Transplante de Córnea/métodos , Cartilagem da Orelha/transplante , Córnea/patologia , Córnea/fisiopatologia , Córnea/cirurgia , Perfuração da Córnea/diagnóstico , Perfuração da Córnea/etiologia , Perfuração da Córnea/fisiopatologia , Perfuração da Córnea/terapia , Técnicas de Diagnóstico Oftalmológico , Humanos , Masculino , Pessoa de Meia-Idade , Transplante Autólogo/métodos , Resultado do Tratamento
4.
Yonsei Med J ; 57(1): 269-71, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26632412

RESUMO

We describe herein a case of an impending corneal perforation with a large descemetocele in a patient with previous penetrating keratoplasty (PKP) that subsequently was treated with an emergent lamellar keratoplasty using frozen preserved cornea. A 76-year-old male patient, who had a PKP, presented with a completely whitish and edematous graft accompanied by large epithelial defects. Although antibiotics and antiviral agents were tried for three days, the corneal stroma abruptly melted, except for the Descemet's membrane and endothelium. Cryopreserved corneal tissue that was kept at -80°C was thawed and sutured on top of the remaining Descemet's membrane and endothelium. Pathological and microbiological tests were conducted using the remaining donor and recipient corneal tissues. After tectonic corneal transplantation on top of a large descemetocele, a healthy graft and relatively clear interfaces between graft-host junctions were maintained without serious adverse reactions throughout 6 month follow-up period. Microbiological evaluations of donor tissue at the time of thawing and tissue preparation were done, and the results were all negative. Tissue that was taken intraoperatively from the recipient cornea also showed negative microbiological results. In conclusion, tectonic lamellar keratoplasty, using cryopreserved corneal tissue, only onto the remaining Descemet's membrane and endothelium in an emergent condition, was a safe and effective treatment.


Assuntos
Córnea/cirurgia , Perfuração da Córnea/cirurgia , Transplante de Córnea/métodos , Criopreservação , Ferimentos Oculares Penetrantes/cirurgia , Doadores de Tecidos , Perfuração da Córnea/patologia , Perfuração da Córnea/fisiopatologia , Ferimentos Oculares Penetrantes/patologia , Ferimentos Oculares Penetrantes/fisiopatologia , Feminino , Humanos , Ceratoplastia Penetrante , Masculino , Pessoa de Meia-Idade , Resultado do Tratamento , Acuidade Visual
5.
Cornea ; 34(11): 1504-7, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26312620

RESUMO

PURPOSE: To report 2 cases of corneal perforation associated with a persistent epithelial defect (PED), which were treated with eye drops containing the fibronectin-derived peptide PHSRN (Pro-His-Ser-Arg-Asn). METHODS: A 67-year-old man and a 58-year-old man presented with corneal perforation associated with a PED caused by lagophthalmos. PHSRN eye drops were administered 4 times a day to both patients. RESULTS: Both patients experienced healing of the epithelial defect and closure of corneal perforation within 3 or 4 days after the onset of PHSRN treatment. Anterior segment optical coherence tomography also revealed recovery of corneal stromal thickness at the lesion site. CONCLUSIONS: PHSRN eye drops were effective for the treatment of corneal perforation due to the PED, with rapid reepithelialization being followed by full restoration of stromal thickness.


Assuntos
Perfuração da Córnea/tratamento farmacológico , Epitélio Anterior/patologia , Fibronectinas/administração & dosagem , Fragmentos de Peptídeos/administração & dosagem , Reepitelização/efeitos dos fármacos , Administração Tópica , Idoso , Doenças da Córnea/complicações , Perfuração da Córnea/etiologia , Perfuração da Córnea/fisiopatologia , Humanos , Masculino , Pessoa de Meia-Idade , Soluções Oftálmicas , Recuperação de Função Fisiológica/fisiologia , Cicatrização/efeitos dos fármacos
6.
Cornea ; 34(6): 658-63, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25811718

RESUMO

PURPOSE: To evaluate the use of lenticules extracted using small incision lenticule extraction (SMILE) surgery as a surgical alternative for wound closure in corneal perforation. METHODS: Corneal lenticules obtained through SMILE surgery with central thickness >100 µm were fixed over corneal perforation sites using 10-0 nylon interrupted stitches. Patients were monitored for a minimum of 1 year and were assessed using slit-lamp microscopy, fluorescein, digital tonometry, and best spectacle-corrected visual acuity (BSCVA) measurements. Postoperative complications throughout the study period were recorded. RESULTS: Corneal perforations were successfully sealed in all 6 patients; 3 patients (50%) exhibited improved postoperative BSCVA. Between 3 and 4 weeks after the operation, part of the lenticules became incorporated into the corneal stroma and complete reepithelialization was achieved. During the follow-up period of 12 months, no evidence of infection, relapse, or perforation was detected in any patient. CONCLUSIONS: These preliminary findings suggest that the use of corneal lenticules may be a safe and effective surgical alternative for corneal perforation closure, with potential clinical application as relatively simple and inexpensive temporary measures to improve the condition of the cornea for further definitive interventions.


Assuntos
Perfuração da Córnea/cirurgia , Substância Própria/transplante , Cirurgia da Córnea a Laser , Adulto , Idoso , Perfuração da Córnea/fisiopatologia , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias , Estudos Prospectivos , Técnicas de Sutura , Preservação de Tecido , Acuidade Visual/fisiologia , Cicatrização/fisiologia
7.
Can J Ophthalmol ; 50(1): 80-4, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25677289

RESUMO

OBJECTIVE: To describe a simplified tectonic lamellar keratoplasty (LK) technique, which can be used to manage full-thickness corneal perforations. DESIGN: Case series demonstrating a new surgical technique. PARTICIPANTS: A 69-year-old female with rosacea keratitis and peripheral corneal perforation; a 49-year-old male with keratoconus, ipsilateral herpes simplex keratitis and an inferonasal corneal perforation; and a 12-year-old female with an apparently spontaneous central corneal perforation. RESULTS: These 3 patients with full-thickness corneal perforations were successfully managed with this automated tectonic LK technique. CONCLUSIONS: Tectonic LK has benefits relative to tectonic penetrating keratoplasty but remains unpopular because it is technically difficult to prepare the host and donor corneas. This tectonic LK technique negates the need for challenging free-hand dissection and gives an excellent donor fit to the host bed. This provides an easier and more effective way to manage corneal perforations.


Assuntos
Perfuração da Córnea/cirurgia , Transplante de Córnea/métodos , Idoso , Criança , Córnea/patologia , Perfuração da Córnea/diagnóstico , Perfuração da Córnea/fisiopatologia , Transplante de Córnea/instrumentação , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Técnicas de Sutura , Doadores de Tecidos , Coleta de Tecidos e Órgãos , Tomografia de Coerência Óptica , Acuidade Visual/fisiologia
8.
Eye Contact Lens ; 40(4): 257-64, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25390550

RESUMO

Acute corneal hydrops (ACH) and perforation in corneal thinning diseases are the consequences of exposure to distending intraocular pressure (IOP) forces that are in excess of corneal resistance to them. Apart from thinning, resistance to these forces may be reduced by disease-related tissue changes, such as corneal scarring, which could lower resistance to IOP. Eye rubbing trauma has sometimes been found to be associated with ACH and perforation. This association is not surprising given that the combination of rubbing-related mechanical tissue trauma and the associated increased distending stress of higher IOP seem likely to increase the risk of complications. Many cases of ACH and perforation are described as spontaneous, but this classification may be the consequence of not considering the multiple potential mechanisms for IOP elevation such as coughing, sneezing, nose blowing, and sneeze suppression in addition to those related to eye rubbing/wiping/massaging/touching as well as changes in body orientation, strenuous exercise, and wearing swimming goggles for example. Classification of ACH or perforation as spontaneous may lead patients to assume that nothing can be done to avoid these complications. Patients with corneal thinning diseases who are counseled regarding the potential precipitating mechanisms for IOP elevation will have the opportunity of reducing exposure to them and the risk of the associated complications. In addition, when ACH or perforation occur, faster resolution of edema and wound healing may depend on reducing potentially exacerbating exposures to mechanisms for IOP elevation.


Assuntos
Edema da Córnea , Perfuração da Córnea , Edema da Córnea/etiologia , Edema da Córnea/fisiopatologia , Lesões da Córnea/complicações , Perfuração da Córnea/etiologia , Perfuração da Córnea/fisiopatologia , Humanos , Pressão Intraocular/fisiologia , Ceratocone/complicações , Fatores de Risco
9.
Cornea ; 32(10): 1326-30, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23974883

RESUMO

PURPOSES: To describe a new technique of scleral tissue augmented cyanoacrylate tissue adhesive (CTA) application and to evaluate its efficacy in noninfectious corneal perforations that measure between 3.5 and 4.5 mm. METHODS: Retrospective noncomparative case series included 16 consecutive patients diagnosed with moderate-sized corneal perforations measuring 3.5 to 4.5 mm who were treated with scleral patch graft augmented glue application. A partial-thickness scleral patch equal to the size of corneal perforation was placed in the corneal perforation site. After the area was sufficiently dried, CTA was applied on the interface of the host cornea and scleral patch. The ability of the scleral patch graft with CTA to seal the perforation and allow complete wound healing was considered a successful outcome. RESULTS: The corneal perforation healed in 14 eyes (87.5%), with a mean of 5.65 weeks (range, 5-9 weeks). One eye (6.25%) developed microperforation that required additional CTA to seal the wound. Three eyes (18.75%) required reapplication of the scleral patch graft and CTA. The 2 eyes (12.50%) that failed scleral patch with CTA were successfully treated with tectonic penetrating keratoplasties. In all 5 eyes (100%), the corneal perforations as a result of Mooren ulcer healed in a mean 5.80 weeks (range, 5-7 weeks). One patient developed retinal detachment with proliferative vitreoretinopathy and cataract after trauma. CONCLUSIONS: Scleral patch graft augmented CTA technique is a successful alternative treatment method to emergent penetrating keratoplasty for corneal perforations that measure 3.5 to 4.5 mm.


Assuntos
Perfuração da Córnea/cirurgia , Cianoacrilatos/uso terapêutico , Esclera/transplante , Adesivos Teciduais/uso terapêutico , Adulto , Perfuração da Córnea/etiologia , Perfuração da Córnea/fisiopatologia , Feminino , Humanos , Ceratoplastia Penetrante , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Doadores de Tecidos , Acuidade Visual/fisiologia , Cicatrização/fisiologia
10.
Invest Ophthalmol Vis Sci ; 54(9): 6262-8, 2013 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-23963165

RESUMO

PURPOSE: Thrombospondin-1 (THBS1) has been suggested as a corneal wound-healing modulator. Therefore, we compromised the integrity of the cornea to elucidate the role of THBS1. METHODS: Full-thickness penetrating corneal incisions (1.5 mm) were created in wild type (WT, 129S2/SvPas) and THBS1-deficient mice (Thbs1⁻/⁻), 129S2/SvPas-Thbs1(tm1Hyn)/Thbs1(tm1Hyn)), and allowed to heal up to 1 month, while being monitored by slit-lamp and intravital corneal examinations. Corneas also were examined by transmission electron microscopy and indirect immunofluorescence. To determine how THBS1 was involved in the healing process, we examined THBS1 and α-smooth muscle actin (SMA), a marker of myofibroblasts and myoepithelial cells. RESULTS: In WT mice by 1 month, corneas appeared transparent with a thin scar, and endothelium and Descemet's membrane (DM) were restored. In contrast, Thbs1⁻/⁻ corneas exhibited chronic edema and persistent opacity after wounding. The DM and endothelium were not restored, and wound contraction was impaired. The THBS1 was localized in epithelial cells at early stages of the healing process, and in the stroma and endothelial cells during later stages. The SMA-positive epithelial cells and myofibroblasts were observed within the healing area at day 4, peaked at day 14, and disappeared at day 30. The SMA-positive cells were reduced greatly in Thbs1⁻/⁻ mice. CONCLUSIONS: In the current study, we demonstrated that corneal restoration is strikingly compromised by a penetrating incision in Thbs1⁻/⁻ mice. The wound results in persistent edema and wound gaping. This appears to be the result of the lack of endothelial migration and DM restoration. In addition, myofibroblast formation is compromised, resulting in the lack of wound contraction.


Assuntos
Perfuração da Córnea/fisiopatologia , Ferimentos Oculares Penetrantes/fisiopatologia , Trombospondina 1/fisiologia , Cicatrização/fisiologia , Actinas/metabolismo , Animais , Perfuração da Córnea/patologia , Substância Própria/metabolismo , Modelos Animais de Doenças , Epitélio Posterior/metabolismo , Ferimentos Oculares Penetrantes/patologia , Feminino , Imunofluorescência , Masculino , Camundongos , Camundongos Knockout , Microscopia Eletrônica de Transmissão , Miofibroblastos/citologia
11.
Vestn Oftalmol ; 129(1): 49-52, 2013 Jan-Feb.
Artigo em Russo | MEDLINE | ID: mdl-23650749

RESUMO

Method for express diagnosis of mechanical corneal injury using infrared spectroscopic analysis of tear fluid is proposed. Differential diagnosis of open and closed corneal injuries in absence of clinical data is presented. Clinical material is provided by patients with open globe injuries--25 patients (25 eyes) and 25 patients (25 eyes) with closed globe injuries. 20 healthy adults (40 eyes) were included into the control group. Proposed method allows to develop treatment strategy, determine extent of surgical interventions in corneal trauma and predict the course of posttraumatic process and complications as well.


Assuntos
Lesões da Córnea , Perfuração da Córnea/diagnóstico , Ferimentos Oculares Penetrantes/diagnóstico , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Ferimentos não Penetrantes/diagnóstico , Adulto , Córnea/fisiopatologia , Perfuração da Córnea/fisiopatologia , Diagnóstico Diferencial , Ferimentos Oculares Penetrantes/fisiopatologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Seleção de Pacientes , Prognóstico , Ferimentos não Penetrantes/fisiopatologia
13.
Eye (Lond) ; 26(11): 1446-50, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22975657

RESUMO

AIMS: To report the surgical outcome of tectonic graft using glycerol-preserved donor corneas to treat perforated keratitis. METHODS: The medical records were reviewed of all patients treated for perforated keratitis using glycerol-preserved corneas at a single institution between 1 July 2004 and 31 June 2010. The clinical features, precipitating factors, adjuvant therapies, and therapeutic outcomes were analyzed. Success was defined as re-epithelialization of the ocular surface without evisceration. RESULTS: Fourteen eyes from 14 patients (6 male and 8 female) were included. Age ranged from 58 to 84 years (average, 70.71 ± 8.52 years) and the follow-up time ranged from 7 to 56 months (mean, 25.35 ± 16.84 months). The culture results showed five bacterial infections, five cases of fungal keratitis, and one mixed infection; the culture results were negative for three patients. Satisfactory anatomical integrity was obtained in eight grafts (57.14%) that healed with neovascularization. Six grafts (48.85%) showed delayed re-epithelialization and were repaired with conjunctival flaps to maintain ocular surface integrity. Three patients developed secondary glaucoma and received trans-scleral cyclophotocoagulation. Thirteen patients had satisfactory anatomical integrity without evisceration or exenteration, while one patient received evisceration at 39-month follow-up because of intractable glaucoma. CONCLUSIONS: Glycerol-preserved donor corneas combined with anterior vitrectomy with or without conjunctival flaps may be effective substitutes for evisceration surgery in patients with perforated keratitis.


Assuntos
Perfuração da Córnea/cirurgia , Transplante de Córnea , Úlcera da Córnea/cirurgia , Infecções Oculares Bacterianas/cirurgia , Infecções Oculares Fúngicas/cirurgia , Glicerol , Preservação de Órgãos/métodos , Idoso , Idoso de 80 Anos ou mais , Antibacterianos/uso terapêutico , Túnica Conjuntiva/cirurgia , Córnea , Perfuração da Córnea/microbiologia , Perfuração da Córnea/fisiopatologia , Úlcera da Córnea/microbiologia , Úlcera da Córnea/fisiopatologia , Infecções Oculares Bacterianas/microbiologia , Infecções Oculares Bacterianas/fisiopatologia , Infecções Oculares Fúngicas/microbiologia , Infecções Oculares Fúngicas/fisiopatologia , Feminino , Humanos , Injeções Intravítreas , Masculino , Pessoa de Meia-Idade , Retalhos Cirúrgicos , Técnicas de Sutura , Resultado do Tratamento , Acuidade Visual/fisiologia , Vitrectomia
15.
Korean J Ophthalmol ; 25(2): 132-5, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21461227

RESUMO

We report a case of tectonic corneal transplantation for impending corneal perforation to preserve anatomic integrity using cryopreserved donor tissue. An 82-year-old woman exhibiting impending corneal perforation suffered from moderate ocular pain in the left eye for one week. After abnormal tissues around the impending perforation area were carefully peeled away using a Crescent blade and Vannas scissors, the patient received tectonic deep anterior lamellar keratoplasty using a cryopreserved cornea stored in Optisol GS® solution at -70℃ for four weeks. At six months after surgery, the cornea remained transparent and restored the normal corneal thickness. There were no complications such as corneal haze or scars, graft rejection, recurrent corneal ulcer, and postoperative rise of intraocular pressure. Cryopreserved donor lamellar tissue is an effective substitute in emergency tectonic lamellar keratoplasty, such as impending corneal perforation and severe necrotic corneal keratitis.


Assuntos
Córnea/cirurgia , Perfuração da Córnea/cirurgia , Transplante de Córnea/métodos , Criopreservação , Ferimentos Oculares Penetrantes/cirurgia , Doadores de Tecidos , Idoso de 80 Anos ou mais , Lesões da Córnea , Perfuração da Córnea/patologia , Perfuração da Córnea/fisiopatologia , Ferimentos Oculares Penetrantes/patologia , Ferimentos Oculares Penetrantes/fisiopatologia , Feminino , Seguimentos , Humanos , Acuidade Visual
16.
Biol Pharm Bull ; 32(9): 1594-9, 2009 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-19721238

RESUMO

The protein sericin is the main constituent of silk. We investigated the effects of sericin on corneal wound healing in Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a model for human type 2 diabetes. Corneal wounds were prepared by removal of the corneal epithelium, and documented using a TRC-50X equipped with a digital camera. Sericin solutions were instilled into the eyes of rats five times a day following corneal abrasion. Plasma glucose and triglycerides were determined using an Accutrend GCT. Cholesterol and insulin were measured using a Cholesterol E-Test Kit and ELISA Insulin Kit, respectively. The plasma levels of glucose, triglycerides, cholesterol and insulin in 38-week-old OLETF rats were significantly higher than in Long-Evans Tokushima Otsuka (LETO) rats used as normal controls, and the rate of corneal wound healing in OLETF rats was slower than in LETO rats. The corneal wounds of rats instilled with saline showed almost complete healing by 72 h after corneal epithelial abrasion. On the other hand, the corneal healing rate of OLETF rats instilled with 10% sericin solution was significantly higher than that of LETO rats instilled with saline, and the wounds showed almost complete healing at 48 h after abrasion. The corneal healing rate increased with increasing sericin concentration. The present study demonstrates that the corneal wound healing rate in OLETF rat is slower than in LETO rats, and the instillation of sericin solution has a potent effect in promoting wound healing and wound-size reduction in LETO and OLETF rats.


Assuntos
Perfuração da Córnea/tratamento farmacológico , Perfuração da Córnea/fisiopatologia , Diabetes Mellitus Tipo 2/tratamento farmacológico , Diabetes Mellitus Tipo 2/fisiopatologia , Sericinas/metabolismo , Sericinas/uso terapêutico , Cicatrização/efeitos dos fármacos , Animais , Perfuração da Córnea/complicações , Diabetes Mellitus Tipo 2/complicações , Modelos Animais de Doenças , Humanos , Masculino , Ratos , Ratos Endogâmicos OLETF , Ratos Long-Evans , Sericinas/farmacologia , Cicatrização/fisiologia
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