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1.
Niger J Clin Pract ; 23(10): 1345-1355, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33047690

RESUMO

Background: Several studies have demonstrated an association between obesity, periodontitis, and exercise. Aims: This study aimed to investigate the effects of regular exercise on obese women with periodontal disease, using serum, saliva, and gingival crevicular fluid (GCF) samples. A before-after study design was adopted to evaluate the effects of 12 weeks of regular exercise on obese women grouped according to periodontal status, without a control group (no exercise). The study sample comprised of 15 patients without periodontitis (NP group) and 10 patients with chronic periodontitis (CP group), from whom periodontal parameters were measured and serum, saliva, and GCF samples were collected. Body mass index (BMI), anthropometric measurements, somatotype-motoric tests, and maximal oxygen consumption (VO2max) were recorded at baseline and after exercise. Subjects and Methods: Med Calc was used for statistical analysis. Results: After exercise, a significant decrease in BMI and a significant increase in VO2max were observed in both groups. A significant decrease in probing depth and clinical attachment loss, serum leptin, GCF tumor necrosis factor-α(TNF-α) and leptin, and a significant increase in GCF resistin were observed in the CP group. A significant decrease in serum TNF-α and leptin levels and a significant increase in serum resistin and GCF TNF-α, leptin, resistin, and adiponectin levels were observed in the NP group. Significant correlations between bleeding on probing and levels of interleukin-1ß and leptin in GCF were observed in the CP group. Conclusions: This study showed that regular exercise exerts different impacts with respect to clinical and biochemical aspects of periodontal and systemic conditions in obese women.


Assuntos
Adipocinas/metabolismo , Periodontite Crônica/complicações , Periodontite Crônica/metabolismo , Exercício Físico/fisiologia , Líquido do Sulco Gengival/química , Obesidade/complicações , Saliva/química , Adipocinas/sangue , Adulto , Índice de Massa Corporal , Estudos de Casos e Controles , Periodontite Crônica/sangue , Feminino , Humanos , Interleucina-1beta/sangue , Interleucina-1beta/metabolismo , Pessoa de Meia-Idade , Obesidade/sangue , Obesidade/metabolismo , Bolsa Periodontal/metabolismo , Resistina/sangue , Resistina/metabolismo , Fator de Necrose Tumoral alfa/sangue , Fator de Necrose Tumoral alfa/metabolismo
2.
J Pathol ; 251(3): 323-335, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-32418202

RESUMO

The lymphatic system plays a crucial role in the maintenance of tissue fluid homeostasis and the immunological response to inflammation. The effects of lymphatic drainage dysfunction on periodontitis have not been well studied. Here we show that lymphatic vessel endothelial receptor 1 (LYVE1)+ /podoplanin (PDPN)+ lymphatic vessels (LVs) are increased in the periodontal tissues, with accumulation close to the alveolar bone surface, in two murine periodontitis models: rheumatoid arthritis (RA)-associated periodontitis and ligature-induced periodontitis. Further, PDPN+ /alpha-smooth muscle actin (αSMA)- lymphatic capillaries are increased, whereas PDPN+ /αSMA+ collecting LVs are decreased significantly in the inflamed periodontal tissues. Both mouse models of periodontitis have delayed lymph flow in periodontal tissues, increased TRAP-positive osteoclasts, and significant alveolar bone loss. Importantly, the local administration of adeno-associated virus for vascular endothelial growth factor C, the major growth factor that promotes lymphangiogenesis, increases the area and number of PDPN+ /αSMA+ collecting LVs, promotes local lymphatic drainage, and reduces alveolar bone loss in both models of periodontitis. Lastly, LYVE1+ /αSMA- lymphatic capillaries are increased, whereas LYVE1+ /αSMA+ collecting LVs are decreased significantly in gingival tissues of patients with chronic periodontitis compared with those of clinically healthy controls. Thus, our findings reveal an important role of local lymphatic drainage in periodontal inflammation-mediated alveolar bone loss. © 2020 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.


Assuntos
Perda do Osso Alveolar/prevenção & controle , Processo Alveolar/metabolismo , Periodontite Crônica/terapia , Terapia Genética , Linfa/metabolismo , Vasos Linfáticos/metabolismo , Maxila/metabolismo , Fator C de Crescimento do Endotélio Vascular/biossíntese , Fator C de Crescimento do Endotélio Vascular/genética , Perda do Osso Alveolar/genética , Perda do Osso Alveolar/metabolismo , Perda do Osso Alveolar/patologia , Processo Alveolar/patologia , Animais , Estudos de Casos e Controles , Periodontite Crônica/genética , Periodontite Crônica/metabolismo , Periodontite Crônica/patologia , Modelos Animais de Doenças , Humanos , Vasos Linfáticos/patologia , Masculino , Maxila/patologia , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Osteoclastos/metabolismo , Osteoclastos/patologia , Fator de Necrose Tumoral alfa/genética
3.
In Vivo ; 34(2): 587-594, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32111757

RESUMO

BACKGROUND/AIM: Epstein-Barr virus (EBV) associates with human chronic periodontitis (CP) progression. We previously demonstrated that butyric acid (BA), produced by periodontopathic bacteria, induced EBV lytic switch activator BZLF1 expression. We investigated whether short chain fatty acids (SCFAs) in CP patients' saliva enabled EBV reactivation. MATERIALS AND METHODS: Saliva was collected from seven CP patients and five periodontally healthy individuals. SCFAs were quantified using HPLC. BZLF1 mRNA and its pertinent protein ZEBRA were determined with Real-time PCR and western blotting. Histone H3 acetylation (AcH3) was further examined. RESULTS: BZLF1 mRNA expression and transcriptional activity in EBV-infected Daudi cells were induced only when treated with the CP saliva. Among SCFAs, BA alone correlated significantly with the BZLF1 transcription (r=0.88; p<0.02). As expected, CP patients' saliva induced AcH3. CONCLUSION: BA in saliva may play a role in EBV reactivation and hence contribute to EBV-related disease progression in CP patients.


Assuntos
Ácido Butírico/metabolismo , Periodontite Crônica/etiologia , Periodontite Crônica/metabolismo , Infecções por Vírus Epstein-Barr/complicações , Infecções por Vírus Epstein-Barr/virologia , Herpesvirus Humano 4/genética , Saliva/metabolismo , Transativadores/genética , Acetilação , Adulto , Idoso , Periodontite Crônica/patologia , Regulação Viral da Expressão Gênica , Histonas/metabolismo , Interações Hospedeiro-Patógeno , Humanos , Pessoa de Meia-Idade , Projetos Piloto
4.
Sci Rep ; 10(1): 1850, 2020 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-32024893

RESUMO

Platelet-rich fibrin (PRF) provides a scaffold for cell migration and growth factors for promoting wound healing and tissue regeneration. Here, we report using PRF in periodontal healing after open flap debridement (OFD) in canine periodontitis. A split-mouth design was performed in twenty dogs. Forty periodontitis surgical sites were randomly categorized into 2 groups; OFD alone and OFD with PRF treatment. Clinical parameters of periodontal pocket depth, gingival index, and the cemento-enamel junction-alveolar bone levels/root length ratio were improved in the OFD + PRF group. The OFD + PRF group also demonstrated a dramatically decreased inflammatory score compared with the OFD group. Collagen accumulation was improved in the OFD + PRF group at later time points compared with baseline. PRF application also significantly reduced inflammatory cytokine expression (TNFA and IL1B), and promoted the expression of collagen production-related genes (COL1A1, COL3A1, and TIMP1) and growth factors (PDGFB, TGFB1, and VEGFA). These findings suggest that PRF combined with OFD provides a new strategy to enhance the overall improvement of canine periodontitis treatment outcomes, especially in terms of inflammation and soft tissue healing. Therefore, PRF use in treating periodontitis could play an important role as a regenerative material to improve canine periodontitis treatment.


Assuntos
Periodontite Crônica/metabolismo , Fibrina/farmacologia , Bolsa Periodontal/tratamento farmacológico , Bolsa Periodontal/metabolismo , Fibrina Rica em Plaquetas/metabolismo , Regeneração/efeitos dos fármacos , Regeneração/fisiologia , Perda do Osso Alveolar/tratamento farmacológico , Perda do Osso Alveolar/metabolismo , Animais , Citocinas/metabolismo , Desbridamento/métodos , Cães , Genes Reguladores/genética , Inflamação/metabolismo , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Índice Periodontal , Retalhos Cirúrgicos/fisiologia , Resultado do Tratamento , Cicatrização/efeitos dos fármacos , Cicatrização/fisiologia
5.
BMC Oral Health ; 20(1): 18, 2020 01 22.
Artigo em Inglês | MEDLINE | ID: mdl-31969148

RESUMO

BACKGROUND: This systematic review aimed to investigate whether non-surgical periodontal therapy (NSPT) can reduce systemic inflammatory levels and improve metabolism in patients undergoing haemodialysis (HD) and/or peritoneal dialysis (PD). METHODS: Electronic databases (PubMed, EMBASE, CENTRAL, CNKI, and WFPD) were searched for randomized controlled trials (RCTs) performed through July 2019. The risk of bias within studies was assessed with the Cochrane Collaboration's risk assessment tool. The systemic inflammatory and metabolic outcomes included the highly sensitive C-reactive protein (hs-CRP), interleukin 6 (IL-6), tumour necrosis factor-a (TNF-a), the albumin (Alb), and lipid metabolite levels. Meta-analyses (MAs) were performed to calculate the overall effect size where appropriate. RESULTS: Five RCTs were included in this study. Compared with untreated periodontitis groups, the dialysis patients after NSPT significantly showed decreased hs-CRP levels at less than or equal to 2 months (standardized mean difference: - 1.53, 95% confidence interval - 2.95 to - 0.11). No significant difference was found in IL-6 and Alb levels following NSPT at either the 3- or 6- month follow-ups. No MAs could be performed on the TNF-a level and the lipid metabolic markers. CONCLUSIONS: NSPT can moderately reduce serum hs-CRP levels in HD and/or PD patients, but did not significantly change IL-6 or Alb levels. For TNF-a and lipid metabolism markers, no sufficient evidence supports that these levels are changed after NSPT. Additional scientific research is necessary to assess the effects of NSPT on systemic inflammation and metabolic parameters in dialysis patients.


Assuntos
Biomarcadores/metabolismo , Periodontite Crônica/terapia , Inflamação/sangue , Interleucina-6/análise , Falência Renal Crônica/sangue , Biomarcadores/análise , Proteína C-Reativa/metabolismo , Periodontite Crônica/metabolismo , Humanos , Inflamação/patologia , Falência Renal Crônica/terapia , Lipídeos/sangue , Diálise Peritoneal , Diálise Renal , Fator de Necrose Tumoral alfa/sangue
6.
Oral Dis ; 26(1): 166-172, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31587460

RESUMO

OBJECTIVE: To determine the effect of smoking on peptidoglycan recognition protein (PGRP)-1 levels in gingival crevicular fluid (GCF) in smokers and non-smokers with chronic periodontitis (CP) following initial periodontal therapy. SUBJECTS AND METHODS: A total of 40 patients with CP (20 smokers (S + CP) and 20 non-smokers (S-CP)) and 40 subjects with healthy periodontium (20 smokers (S + PH) and 20 non-smokers (S-PH)), comprising 80 subjects, were included in this study. Baseline GCF samples were obtained from all subjects, and clinical periodontal measurements were recorded. In patients who had received initial periodontal therapy, GCF samples were obtained and all clinical periodontal measurements were recorded again during the 6th-8th weeks. GCF PGRP-1 levels were analyzed by enzyme-linked immunosorbent assay. RESULTS: At baseline, GCF PGRP-1 levels were significantly higher in both groups with CP than in both groups with healthy periodontium, whereas these levels were significantly lower in S + CP than in S-CP. GCF PGRP-1 levels decreased significantly in both CP groups after periodontal therapy, and this reduction was significantly greater in non-smokers than in smokers. CONCLUSION: Smoking might have a suppressive effect on GCF PGRP-1 levels in CP. Initial periodontal therapy is effective in decreasing GCF PGRP-1 levels in both smokers and non-smokers with CP.


Assuntos
Periodontite Crônica/metabolismo , Citocinas/metabolismo , Líquido do Sulco Gengival/química , Fumar/efeitos adversos , Adulto , Estudos de Casos e Controles , Periodontite Crônica/terapia , Raspagem Dentária , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Índice Periodontal , Aplainamento Radicular , Adulto Jovem
7.
Dokl Biol Sci ; 488(1): 156-159, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31732903

RESUMO

Morphological and immunohistochemical examination was made on 24 gum biopsies obtained from 35- to 60-year-old patients with diagnosis of partial secondary adentia, chronic generalized moderate to severe periodontitis (19 patients), as well as on the biopsy samples from five patients without pathological periodontal changes who underwent dental implantation. Serial paraffin sections were treated with antibodies against Ki-67, VEGF, and SMA. In patients with severe chronic periodontitis, a high proliferative activity of epithelium indicative of hyperplastic changes was observed, as well as a reduced number of the SMA-positive cells and actual absence of the SMA-positive cell couplings associated with the "growth zones" in tissues, which testifies indirectly to a lower tissue regenerative capacity. Hence, before dental implantation, additional anti-inflammatory and pro-regenerative treatment is required.


Assuntos
Periodontite Crônica/metabolismo , Implantação Dentária , Antígeno Ki-67/metabolismo , Periodonto/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo , Adulto , Periodontite Crônica/patologia , Feminino , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Periodonto/patologia , Valor Preditivo dos Testes
8.
In Vivo ; 33(6): 1793-1800, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31662504

RESUMO

BACKGROUND/AIM: Human chronic periodontitis is a major health problem. Although some oral bacteria have been reported to be putative pathogens, Epstein-Barr virus (EBV) is reported to be associated with the progression of periodontitis. However, the role of EBV in the aetiology of periodontitis is unknown. Therefore, we investigated periodontal pathogenesis of EBV to confirm whether EBV-encoded latent membrane protein 1 (LMP1) induces Interleukin-8 (IL8) production in human gingival cells. MATERIALS AND METHODS: Real-time polymerase chain reaction, luciferase assay, enzyme-linked immunosorbent assay (ELISA), and western blotting were performed for determining IL8 mRNA expression, nuclear factor kappa B (NF-ĸB) transcription, IL8 production, and the phosphorylation of NF-ĸB p65 and Inhibitor of kappa B alpha (IĸBα), respectively, in Ca9-22 human gingival epithelial cells. Two LMP1 mutants lacking C-terminal activating region (CATR) domains responsible for activating NF-ĸB were used. RESULTS: Extremely high IL8 production was induced by LMP1 in time- and dose-dependent manner, where simultaneous phosphorylation of NF-κB p65 and IĸBα and transcription of NF-ĸB were observed. On the contrary, IL8 production and NF-ĸB transcription were drastically inhibited by dominant negative mutant of IĸBα. Moreover, the LMP1 mutants failed to induce IL8 production. CONCLUSION: Our findings suggest that due to CATR domains, LMP1 contributes to the progression of periodontitis via IL8 production attributable to NF-ĸB activation.


Assuntos
Periodontite Crônica/metabolismo , Células Epiteliais/metabolismo , Epitélio/metabolismo , Gengiva/metabolismo , Herpesvirus Humano 4/metabolismo , Interleucina-8/metabolismo , Proteínas da Matriz Viral/metabolismo , Linhagem Celular , Células Epiteliais/virologia , Epitélio/virologia , Gengiva/virologia , Humanos , NF-kappa B/metabolismo , Transdução de Sinais/fisiologia , Fator de Transcrição RelA/metabolismo
9.
Metabolomics ; 15(11): 141, 2019 10 14.
Artigo em Inglês | MEDLINE | ID: mdl-31612356

RESUMO

INTRODUCTION: Oral microflora is a well-orchestrated and acts as a sequential defense mechanism for any infection related to oral disease. Chronic periodontitis is a disease of a microbial challenge to symbiosis and homeostasis. Periodontal surgery is the most promising cure with repair process during periodontal regeneration. It has an encouraging outcome in terms of early recovery biomarkers. OBJECTIVE: Saliva of periodontal surgery subjects with the chronic periodontitis have been evaluated by 1H NMR spectroscopy in search of possible early metabolic differences that could be obtained in order to see the eradication of disease which favours the symbiotic condition. METHOD: The study employed 1H NMR spectroscopy on 176 human saliva samples in search of distinctive differences and their spectral data were further subjected to multivariate and quantitative analysis. RESULT: The 1H NMR study of periodontal surgery samples shows clear demarcation and profound metabolic differences when compared with the diseased condition. Several metabolites such as lactate, ethanol, succinate, and glutamate were found to be of higher significance in periodontal surgery in contrast to chronic periodontitis subjects. The PLS-DA model of the studied group resulted in R2 of 0.83 and Q2 of 0.70. CONCLUSION: Significant metabolites could be considered as early repair markers for chronic periodontitis disease as they are being restored to achieve symbiosis. The study, therefore, concluded the early recovery process of the diseased subjects with the restoration of possible metabolomic profile similar to the healthy controls.


Assuntos
Periodontite Crônica/metabolismo , Metabolômica , Saliva/química , Biomarcadores/análise , Biomarcadores/metabolismo , Periodontite Crônica/diagnóstico , Periodontite Crônica/cirurgia , Humanos , Análise Multivariada , Espectroscopia de Prótons por Ressonância Magnética
10.
BMC Oral Health ; 19(1): 218, 2019 10 11.
Artigo em Inglês | MEDLINE | ID: mdl-31604439

RESUMO

BACKGROUND: The purpose of this study was to investigate gingival crevicular fluid (GCF) and serum folate-receptor 1 (FOLR1) levels in subjects with different periodontal status. METHODS: The study consists of three groups: Healthy group (n = 15), gingivitis group (n = 15) and chronic periodontitis group (n = 15). Clinical periodontal parameters including probing pocket depth (PPD), clinical attachment level (CAL), gingival index (GI) and bleeding on probing (BOP) were assessed. GCF and serum samples were collected from each patient and were analyzed FOLR1 levels by enzyme-linked immunosorbent assay. RESULTS: The values of FOLR1 in GCF were higher in gingivitis and periodontitis groups than among patient in control group (p < 0.016). Serum FOLR1 levels showed no significant difference between the groups. A significant correlation was observed between FOLR1 levels of GCF and BOP (p < 0.05). CONCLUSIONS: Our preliminary data suggest that FOLR1 is not useful in monitoring the periodontal disease. Further studies are necessary to clarify the role, regulation and function of folate and it's receptors in the pathogenesis of periodontal disease.


Assuntos
Periodontite Crônica/metabolismo , Receptor 1 de Folato/sangue , Líquido do Sulco Gengival/química , Gengivite/metabolismo , Periodontite Crônica/sangue , Feminino , Ácido Fólico , Líquido do Sulco Gengival/metabolismo , Gengivite/sangue , Humanos , Masculino , Índice Periodontal , Periodontite , Projetos Piloto
11.
Artigo em Inglês | MEDLINE | ID: mdl-31605582

RESUMO

INTRODUCTION: The present study aimed to assess the presence of main types of microorganisms involved in the aetiopathogenesis of chronic periodontitis with PCR technique and determinates the presence of composite IL-1 genotype and their associations with founded bacteria. MATERIAL AND METHOD: The examined group was consisted from 20 subjects with diagnosed chronic periodontitis and 20 healthy control without periodontitis. Clinical parameters like gingival index (GI), plaque index (PI), bleeding on probing (BOP), periodontal pocket depth (PPD) and clinical attachment lost (CAL) were determinates. Subgingival dental plaque was collected using a sterilized paper point. We used Parodontose Plus test, reverse hybridization kit, for the detection of periodontal marker bacteria, as well as for the detection of composite Interleukin -1 Genotype Results: The most present bacterial species detected from subgingival dental plaque was Treponema denticola and Porfiromonas gingivalis which was present in 65% of examined patients. In relation to the presence of positive genotype in patients, there was no significant difference between the test and control group for p> 0.05 (p = 1.00). For χ2=8,17 (p=0,06, p<0,05) there is an association between Prevotella intermedia, and composite genotype. Between positive genotype and analyzed bacterial species A. actinomycetem comitans for p> 0.05 (p = 1.00), P. gingivalis for p> 0.05 (p = 0.16), T. Forsythia for p> 0.05 (p = 0.20), T. Denticola for p> 0.05 (p = 0.64) no association was found. CONCLUSION: This investigations confirmed the strong association of these five examined periopathogenes with periodontitis.


Assuntos
Periodontite Crônica/microbiologia , Placa Dentária/microbiologia , Interleucina-1/genética , Adulto , Aggregatibacter actinomycetemcomitans/genética , Aggregatibacter actinomycetemcomitans/crescimento & desenvolvimento , Aggregatibacter actinomycetemcomitans/isolamento & purificação , Periodontite Crônica/metabolismo , Placa Dentária/metabolismo , Índice de Placa Dentária , Genótipo , Humanos , Pessoa de Meia-Idade , Índice Periodontal , Bolsa Periodontal/microbiologia , Reação em Cadeia da Polimerase/métodos , Porphyromonas gingivalis/genética , Porphyromonas gingivalis/crescimento & desenvolvimento , Porphyromonas gingivalis/isolamento & purificação , Prevotella intermedia/genética , Prevotella intermedia/crescimento & desenvolvimento , Prevotella intermedia/isolamento & purificação , Treponema denticola/genética , Treponema denticola/crescimento & desenvolvimento , Treponema denticola/isolamento & purificação
12.
Clin Rheumatol ; 38(11): 3139-3143, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31372850

RESUMO

BACKGROUND: Visfatin is an adipokine and has a crucial role in pro-inflammatory response. The aim of this study was investigating the visfatin levels of gingival crevicular fluid (GCF) in patients with systemic lupus erythematosus (SLE) and chronic periodontitis and healthy subjects. MATERIALS AND METHODS: Sixty non-obese females were selected based on their clinical parameters into four groups: 15 healthy subjects (H-H), 15 systemically healthy individuals with chronic periodontitis (H-CP), 15 SLE patient with CP (SLE-CP), and 15 SLE patients without CP (SLE-H). GCF samples were collected to estimate the levels of visfatin using enzyme-linked immunosorbent assay (ELISA). RESULTS: Investigating the amount of visfatin in the GCF showed that there is a significant difference between visfatin amount of GCF in SLE patients and chronic periodontitis (L-CP) in comparison with other groups (P < 0.001). CONCLUSION: Visfatin levels have correlated positively with all the clinical periodontal parameters and its levels in (L-CP) group are highest in comparative with other groups. This finding suggests visfatin has a possible role in association between these two inflammatory conditions. Key Point • Visfatin in systemic lupus erythematosus.


Assuntos
Periodontite Crônica/metabolismo , Líquido do Sulco Gengival/metabolismo , Lúpus Eritematoso Sistêmico/metabolismo , Nicotinamida Fosforribosiltransferase/metabolismo , Adulto , Estudos de Casos e Controles , Feminino , Humanos , Pessoa de Meia-Idade
13.
Diabetes Metab Syndr ; 13(4): 2769-2774, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31405706

RESUMO

BACKGROUND: Diabetes mellitus and periodontal disease are two common and chronic diseases with bidirectional relationship influence public health and quality of life. The aims of this study was to study the impact of resveratrol supplementation in adjunct with non-surgical periodontal therapy on inflammatory, antioxidant, and periodontal markers in patients with type 2 diabetes with periodontal disease. MATERIALS AND METHODS: In this randomized clinical trial, 43 patients with diabetes and chronic periodontitis were randomly allocated into two intervention and control groups receiving either resveratrol supplements or placebo for 4 weeks. Serum levels of interleukin 6 (IL6), tumor necrosis factor α (TNFα), total antioxidant capacity (TAC) and clinical attachment loss (CAL) as the main index of periodontal marker were measured pre-intervention and post-intervention. RESULTS: In the intervention group, the mean serum level of IL6 was reduced significantly (P = 0.039) post-intervention (2.19 ±â€¯1.09 and 1.58 ±â€¯1.06). No significant differences were seen in the mean levels of IL6, TNFα, TAC and CAL between two groups post-intervention. CONCLUSIONS: It is suggested that daily consumption of resveratrol supplement may not change TNFα, TAC and CAL, but it would be beneficial in reducing serum levels of IL6. Therefore, further studies are suggested to investigate the effects of resveratrol supplementation along with NST on periodontal status.


Assuntos
Antioxidantes/metabolismo , Biomarcadores/metabolismo , Periodontite Crônica/metabolismo , Diabetes Mellitus Tipo 2/metabolismo , Suplementos Nutricionais , Mediadores da Inflamação/metabolismo , Resveratrol/uso terapêutico , Adulto , Antioxidantes/uso terapêutico , Estudos de Casos e Controles , Periodontite Crônica/complicações , Periodontite Crônica/tratamento farmacológico , Periodontite Crônica/patologia , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/tratamento farmacológico , Diabetes Mellitus Tipo 2/patologia , Método Duplo-Cego , Feminino , Seguimentos , Humanos , Inflamação/prevenção & controle , Masculino , Pessoa de Meia-Idade , Prognóstico
14.
In Vivo ; 33(4): 1165-1174, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31280206

RESUMO

Chronic periodontitis is an inflammatory disease of tooth-supporting tissues associated with Porphyromonas gingivalis. Expansion and invasion of this bacterium into the periodontium is associated with changes in the metabolome of the oral cavity. MATERIALS AND METHODS: Metabolomics analysis of mouth washout and tongue swab samples based on proton nuclear magnetic resonance (1H-NMR) method was employed to determine metabolic status of the oral cavity in chronic periodontal disease. RESULTS: Mouth washout extracts contained a total of 23 metabolites and tongue swab extracts contained 17. Identified metabolites partially overlap with the content of saliva and gingival crevicular fluid. The colonization of the oral cavity of patients with periodontitis by bacteria was manifested in the change in levels of eight metabolites. CONCLUSION: NMR-based metabolomics analysis is a potentially useful methodological approach for monitoring the pathological processes observed in the oral cavity in the course of periodontitis.


Assuntos
Periodontite Crônica/metabolismo , Metaboloma , Metabolômica , Boca/metabolismo , Biomarcadores , Periodontite Crônica/etiologia , Periodontite Crônica/patologia , Feminino , Líquido do Sulco Gengival , Humanos , Espectroscopia de Ressonância Magnética/métodos , Masculino , Curva ROC , Saliva
15.
Stomatologiia (Mosk) ; 98(2): 27-30, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31089116

RESUMO

The aim of the work was to assess the balance between osteosynthetic and osteodestructive processes in the oral cavity in patients with chronic generalized periodontitis (CGP) of moderate and severe severity in the dynamics of a remote period of patchwork using various bone-plastic materials (BPM) based on xenogeneic hydroxyapatite (HA), ß-tricalcium phosphate (TCP). 123 patients with CGP were examined. Depending on the type of BPM, two groups were distinguished by composition in the course of patchwork operations. Patients of group 1 (n=61) received BPM on the basis of xenogeneic HA, 2 groups (n=62) on the basis of ß-TCP. Initially and after surgery at 8 and 12 months in the oral fluid, the concentration of osteoprotegerin (OPG), the ligand of the soluble activator of the kappa B nucleation factor (sRANKL), the bone isoenzyme of alkaline phosphatase (BAP), was determined by an immunoenzyme method. As a result of the study, it was found that in patients with moderate-bodied CGP after surgical treatment, restriction of osteoclast activity with a decrease in sRANKL due to an increase in OPG in the oral fluid, an increase in osteoblast activity with BAP secretion is most pronounced when using ß-TCP-based BPM at 8 months. After surgery with an average severity of the disease. Less pronounced changes in bone metabolism in the oral cavity are detected with the use of BPM on the basis of HA. In severe CGP, changes in bone homeostasis in the oral cavity after scrappy operations with BPM insertion based on TCP or HA are not expressed and are observed only after 12 months. Thus, the most effective changes in bone metabolism develop with the use of TCP as BPM.


Assuntos
Biomarcadores , Periodontite Crônica , Biomarcadores/análise , Osso e Ossos , Periodontite Crônica/metabolismo , Durapatita , Humanos
16.
Ann Afr Med ; 18(2): 92-96, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31070151

RESUMO

Background: Increased plasma adrenomedullin levels are usually observed in certain conditions such as diabetes mellitus (DM). Aims and Objectives: To gauge adrenomedullin values in the gingival crevicular fluid (GCF) of healthy individuals, chronic periodontitis (CP) patients with and without DM, and diabetics without any periodontal involvement, prior and after periodontal therapy. Materials and Methods: A total of 120 subjects were segregated into four categories of 30 each: Group 1 (healthy subjects), Group 2 (CP without DM), Group 3 (patients with CP and DM), and Group 4 (diabetics without any periodontal involvement). GCF was collected from all the patients. Nonsurgical periodontal therapy was done in Group 2 and Group 3 patients, followed up for 6 weeks, and GCF was obtained again from the same site. Enzyme-linked immunosorbent assay was used to evaluate the samples. Results: Adrenomedullin level in the GCF was high in diabetics without any periodontal involvement than in healthy individuals and significantly elevated in those with CP and DM. Reduced adrenomedullin was noted 6 weeks postperiodontal therapy. Conclusion: Adrenomedullin levels being higher in diabetics without any periodontal involvement than healthy controls suggest a link between DM and adrenomedullin. Furthermore, infection plays a noteworthy role in the elevation of adrenomedullin, indicating its role in periodontal disease progression.


Assuntos
Adrenomedulina/metabolismo , Periodontite Crônica/complicações , Periodontite Crônica/metabolismo , Raspagem Dentária , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/metabolismo , Líquido do Sulco Gengival/química , Aplainamento Radicular , Adrenomedulina/análise , Adrenomedulina/sangue , Adulto , Estudos de Casos e Controles , Diabetes Mellitus Tipo 2/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos
17.
Mediators Inflamm ; 2019: 1029857, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31049022

RESUMO

Periodontitis is characterized by a chronic inflammation produced in response to a disease-associated multispecies bacterial community in the subgingival region. Although the inflammatory processes occur locally in the oral cavity, several studies have determined that inflammatory mediators produced during periodontitis, as well as subgingival species and bacterial components, can disseminate from the oral cavity, contributing therefore, to various extraoral diseases like cancer. Interestingly, carcinogenesis associated with periodontal species has been observed in both the oral cavity and in extra oral sites. In this review, several studies were summarized showing a strong association between orodigestive cancers and poor oral health, presence of periodontitis-associated bacteria, tooth loss, and clinical signs of periodontitis. Proinflammatory pathways were also summarized. Such pathways are activated either by mono- or polymicrobial infections, resulting in an increase in the expression of proinflammatory molecules such as IL-6, IL-8, IL-1ß, and TNF-α. In addition, it has been shown that several periodontitis-associated species induce the expression of genes related to cell proliferation, cell cycle, apoptosis, transport, and immune and inflammatory responses. Intriguingly, many of these pathways are linked to carcinogenesis. Among them, the activation of Toll-like receptors (TLRs) and antiapoptotic pathways (such as the PI3K/Akt, JAK/STAT, and MAPK pathways), the reduction of proapoptotic protein expression, the increase in cell migration and invasion, and the enhancement in metastasis are addressed. Considering that periodontitis is a polymicrobial disease, it is likely that mixed species promote carcinogenesis both in the oral cavity and in extra oral tissues and probably-as observed in periodontitis-synergistic and/or antagonistic interactions occur between microbes in the community. To date, a good amount of studies has allowed us to understand how monospecies infections activate pathways involved in tumorigenesis; however, more studies are needed to determine the combined effect of oral species in carcinogenesis.


Assuntos
Carcinogênese/imunologia , Carcinogênese/metabolismo , Periodontite Crônica/imunologia , Periodontite Crônica/metabolismo , Inflamação/metabolismo , Animais , Citocinas/metabolismo , Humanos
18.
Genet Test Mol Biomarkers ; 23(4): 255-263, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30986096

RESUMO

AIMS: Periodontitis results from the presence of periodontopathogenic bacterial activity in the region of the gingival sulcus promoting tissue degradation and alveolar bone resorption. Biochemical analysis of the saliva can be used as a less invasive method for disease prognosis. This study aimed to evaluate the relationship between biochemical protein levels in the saliva sample of patients with chronic periodontitis and healthy patients. MATERIALS AND METHODS: A literature review was performed using electronic databases (Cochrane Library, Google Scholar, MEDLINE, PubMed, and Web of Science) for studies published before July 2, 2016. The abstracts were evaluated, and the data extraction was performed by two calibrated examiners. The mean difference, and heterogeneity were calculated, and funnel plots were produced. RESULTS: Twenty case-control studies were selected with 2436 patients with chronic periodontitis and 1787 controls. The meta-analysis showed that increased levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and acid phosphatase (ACP) were all associated with periodontitis (p < 0.05), while blood urea nitrogen (BUN) and osteoprotegerin (OPG) levels did not show statistical differences between cases and controls (p > 0.05). CONCLUSIONS: This meta-analysis evidenced that increased levels of AST, ALT, CK, gama glutamil transferase (GGT), LDH, ALP, and ACP are associated in patients with chronic periodontitis, while BUN and OPG level in saliva did not present differences between groups.


Assuntos
Periodontite Crônica/metabolismo , Periodontite Crônica/fisiopatologia , Fosfatase Ácida/análise , Fosfatase Ácida/sangue , Adulto , Alanina Transaminase/análise , Alanina Transaminase/sangue , Fosfatase Alcalina/análise , Fosfatase Alcalina/sangue , Aspartato Aminotransferases/análise , Aspartato Aminotransferases/sangue , Estudos de Casos e Controles , Creatina Quinase/análise , Creatina Quinase/sangue , Feminino , Humanos , L-Lactato Desidrogenase/análise , L-Lactato Desidrogenase/sangue , Masculino , Saliva/química
19.
J Periodontal Res ; 54(5): 555-565, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30982988

RESUMO

BACKGROUND AND OBJECTIVE: Wnt signaling pathways regulate osteoblast differentiation and bone formation and are associated with inflammatory responses driven by innate and adaptive immunity via the NF-κB pathway. The aim of this study was to compare the levels of sclerostin (SOST), WNT-5a, and TNF-α between chronic periodontitis and periodontally healthy sites and determine their value as diagnostic markers of chronic periodontitis. MATERIAL AND METHODS: In a cross-sectional assessment 25 chronic periodontitis cases and 25 periodontally healthy controls were selected upon clinical and radiographic periodontal evaluation. Gingival crevicular fluid (GCF) was collected cross-sectionally from diseased and healthy sites in periodontitis patients and from healthy sites in each control subject. In a subgroup analysis, ten patients with generalized moderate and severe chronic periodontitis and ten generalized periodontally healthy individuals were included. The protein levels of SOST, WNT-5a, and TNF-α in GCF were measured by sandwich ELISA. The Shapiro-Wilk test was utilized to assess the normality of the distribution and non-parametric comparisons were performed. RESULTS: The protein levels of SOST were significantly higher in the generalized moderate and severe chronic periodontitis subgroup when compared to the generalized healthy (P = 0.002), while the WNT-5a and TNF-α GCF total amounts were similar (P > 0.05). Diseased sites in the periodontitis patients exhibited significantly higher total protein levels of WNT-5a than in healthy sites (P = 0.017), whereas no differences were detected for SOST and TNF-α (P > 0.05). The total protein levels of SOST, WNT-5a, and TNF-α in GCF were similar in periodontitis and non-periodontitis patients (P > 0.05). CONCLUSIONS: Sclerostin and WNT-5a gingival protein levels demonstrated a high diagnostic value for generalized moderate and severe chronic periodontitis, while a low accuracy was detected for localized chronic periodontitis.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Produtos Biológicos , Periodontite Crônica , Proteína Wnt-5a , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Produtos Biológicos/metabolismo , Periodontite Crônica/diagnóstico , Periodontite Crônica/metabolismo , Estudos Transversais , Gengiva , Líquido do Sulco Gengival , Humanos , Fator de Necrose Tumoral alfa , Proteína Wnt-5a/metabolismo
20.
Clin Chim Acta ; 495: 278-286, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31026423

RESUMO

BACKGROUND: Certain candidate biomarkers for periodontal diseases in saliva, gingival crevicular fluid (GCF), and serum were reported by some previous studies, but little evidence was obtained in their potentiality for screening patients with periodontal diseases. METHODS: Unstimulated whole saliva, GCF, and serum samples, which were collected from 17 patients with chronic periodontitis, 17 with gingivitis, and 16 periodontally healthy persons as control, were analysed by MALDI-TOF MS. Cluster analysis and receiver operating characteristic (ROC) curve analysis were carried out to evaluate the ability of candidate peptides to distinguish patients with periodontal diseases from healthy subjects. Nano-LC/ESI-MS/MS was performed to identify possible proteins that these peptides might derive from. RESULTS: Most of the differentially expressed peptides exhibited an increase in participants with chronic periodontitis and gingivitis compared with healthy controls. Cluster analysis showed a good clustering capacity between chronic periodontitis and healthy controls. Most AUCs for differentially expressed peptides were >0.7, whereas some peptides from GCF and serum even exhibited AUCs of 0.9-1.0. CONCLUSIONS: Some peptides in saliva, GCF, and serum act as biomarkers for chronic periodontitis and gingivitis, which have certain potentiality for screening patients with periodontal diseases and distinguishing them from healthy individuals in a comparatively large population by mass spectrometry.


Assuntos
Periodontite Crônica/metabolismo , Gengivite/metabolismo , Programas de Rastreamento/métodos , Saliva/química , Adulto , Biomarcadores/análise , Estudos de Casos e Controles , Periodontite Crônica/sangue , Periodontite Crônica/diagnóstico , Análise por Conglomerados , Feminino , Líquido do Sulco Gengival/química , Gengivite/sangue , Gengivite/diagnóstico , Humanos , Masculino , Espectrometria de Massas/métodos , Pessoa de Meia-Idade , Proteínas/análise , Proteínas/metabolismo , Curva ROC
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