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1.
Artigo em Inglês | MEDLINE | ID: mdl-33528452

RESUMO

The syndrome known as posterior bite collapse (PBC) has taken on multiple definitions over the years since its first introduction in 1964 by Morton Amsterdam and Leonard Abrams. In 2017, the World Workshop in the Classification of Periodontal and Peri-implant Diseases and Conditions proposed a staging system for periodontitis, defined by severity and extent of periodontal breakdown. Within this staging system, Stage IV periodontitis can include PBC. However, without a clear delineation regarding the clinical presentation or pathogenesis of PBC, this further obfuscates its definition. It is therefore the goal of this article to reexamine the original definition of PBC as defined by Amsterdam and Abrams, present an updated definition, and propose a clinical grading system of PBC to coincide with the 2017 staging of periodontitis.


Assuntos
Doenças Periodontais , Periodontite , Humanos , Periodontite/diagnóstico
2.
BMJ Case Rep ; 14(2)2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33526536

RESUMO

A 49-year-old man with a 37.5 pack-year smoking history presented with a suspected neoplasm of the right lung following the discovery of a metabolically active mass on positron emission tomography-CT imaging. The patient, who demonstrated poor oral hygiene, had a history of irregular problem-oriented dental visitation. Having excluded malignancy through histologic investigations, Aggregatibacter actinomycetemcomitans-a well-established periodontal pathogen-was subsequently cultured from his pulmonary aspirate. The patient was therefore managed with systemic antimicrobials and adjunctive dental extractions to eliminate the likely source of infection, whereafter the mass resolved without complication. This case corroborates previous reports of extraoral isolation of A. actinomycetemcomitans, which may mimic cancer clinically and radiographically. While a definitive causative link between untreated periodontitis and systemic infection remains to be elucidated, such cases present a compelling argument in favour of promoting oral health to prevent systemic disease.


Assuntos
Aggregatibacter actinomycetemcomitans/isolamento & purificação , Diagnóstico Diferencial , Abscesso Pulmonar/diagnóstico por imagem , Neoplasias Pulmonares/diagnóstico , Infecções por Pasteurellaceae/diagnóstico , Periodontite/diagnóstico , Nódulo Pulmonar Solitário/diagnóstico por imagem , Biópsia por Agulha Fina , Secções Congeladas , Humanos , Abscesso Pulmonar/tratamento farmacológico , Masculino , Pessoa de Meia-Idade , Infecções por Pasteurellaceae/tratamento farmacológico , Periodontite/complicações , Periodontite/terapia , Tomografia Computadorizada com Tomografia por Emissão de Pósitrons , Radiografia Panorâmica , Radiografia Torácica , Cirurgia Torácica Vídeoassistida , Tomografia Computadorizada por Raios X
3.
Support Care Cancer ; 29(1): 135-143, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32323001

RESUMO

PURPOSE: This retrospective study investigated the effect of perioperative oral care intervention on postoperative outcomes in patients undergoing lung cancer resection, in terms of the length of postoperative hospital stay and the incidence of postoperative respiratory infections. METHODS: In total, 585 patients underwent lung resection for lung cancer, 397 received perioperative oral care intervention, whereas the remaining 188 did not. This study retrospectively investigated the demographic and clinical characteristics (including postoperative complications and postoperative hospital stay) of each group. To determine whether perioperative oral care intervention was independently associated with either postoperative hospital stay or postoperative respiratory infections, multivariate analysis, multiple regression analysis, and multivariate logistic regression analysis were conducted. RESULTS: Parameters significantly associated with a prolonged postoperative hospital stay in lung cancer surgery patients were older age, postoperative complications, increased intraoperative bleeding, more invasive operative approach (e.g., open surgery), and lack of perioperative oral care intervention (standard partial regression coefficient (ß) = 0.083, p = 0.027). Furthermore, older age and longer operative time were significant independent risk factors for the occurrence of postoperative respiratory infections. Lack of perioperative oral care intervention was a potential risk factor for the occurrence of postoperative respiratory infections, although not statistically significant (odds ratio = 2.448, 95% confidence interval = 0.966-6.204, p = 0.059). CONCLUSION: These results highlight the importance of perioperative oral care intervention prior to lung cancer surgery, in order to shorten postoperative hospital stay and reduce the risk of postoperative respiratory infections.


Assuntos
Cárie Dentária/terapia , Neoplasias Pulmonares/cirurgia , Periodontite/terapia , Assistência Perioperatória/métodos , Complicações Pós-Operatórias/prevenção & controle , Infecções Respiratórias/prevenção & controle , Adulto , Idoso , Cárie Dentária/diagnóstico , Empiema/tratamento farmacológico , Empiema/prevenção & controle , Feminino , Humanos , Tempo de Internação/estatística & dados numéricos , Pulmão/patologia , Neoplasias Pulmonares/complicações , Masculino , Pessoa de Meia-Idade , Razão de Chances , Saúde Bucal , Pacientes , Periodontite/diagnóstico , Pneumonia/tratamento farmacológico , Pneumonia/prevenção & controle , Complicações Pós-Operatórias/tratamento farmacológico , Complicações Pós-Operatórias/microbiologia , Infecções Respiratórias/tratamento farmacológico , Estudos Retrospectivos , Fatores de Risco
4.
Artigo em Inglês | MEDLINE | ID: mdl-33077409

RESUMO

OBJECTIVE: We sought new markers to predict oral malodor. STUDY DESIGN: Seventy-five adults complaining of oral malodor were classified into 3 groups clinically: no oral malodor, physiologic oral malodor, and periodontitis-derived oral malodor. In addition to conventional clinical parameters, 7 salivary components, occlusal force, and lip-closing force were compared among the groups. RESULTS: Concerning the salivary components, cariogenic bacteria, occult blood, leukocytes, and ammonia differed significantly among the groups. Multiple logistic regression analyses indicated that tongue-coating scores and ammonia levels were significantly associated with genuine oral malodor, including physiologic oral malodor and periodontitis-derived oral malodor, and the tongue-coating score, plaque index, and occult blood level were significantly associated with periodontitis-derived oral malodor. Occlusal force and lip-closing force did not differ among the groups. However, there was a statistically significant interaction between occlusal force and lip-closing force in oral malodor in women (P = .019). CONCLUSIONS: Novel salivary markers, ammonia levels, and occult blood levels may predict genuine oral malodor and periodontitis-derived oral malodor, respectively. An interaction effect between occlusal force and lip-closing force on oral malodor was identified in women.


Assuntos
Halitose , Periodontite , Adulto , Biomarcadores , Índice de Placa Dentária , Feminino , Halitose/diagnóstico , Humanos , Periodontite/diagnóstico , Língua
5.
Cell Prolif ; 53(10): e12886, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32794619

RESUMO

OBJECTIVES: Diabetes aggravates the risk and severity of periodontitis, but the specific mechanism remains confused. Complement 3 (C3) is closely related to complications of type 2 diabetes (T2DM). In the present study, we concentrated on whether C3 mediates the development of periodontitis in T2DM. MATERIALS AND METHODS: Levels of C3 in blood and gingival crevicular fluid (GCF) of patients were measured first. A C3-knockout diabetic mouse model was established, real-time PCR, Western blotting and histological investigation were performed to evaluate the progress of periodontitis. Microcomputed tomography (micro-CT) and TRAP staining were performed to detect alveolar bone resorption. Immunofluorescence was performed to detect polarization of macrophages. RESULTS: Our data showed that C3 levels were elevated in the blood and GCF of T2DM patients compared with non-diabetic individuals. Increased C3 was closely related to the upregulation of inflammatory cytokines including interleukin (IL)-1, IL-6 and tumour necrosis factor-alpha (TNF-α), as well as the decline of the bone volume density (BMD) and bone volume over total volume (BV/TV) of the alveolar bones in diabetic mice. The deletion of C3 inhibited inflammatory cytokines and rescued the decreased BMD and BV/TV of the alveolar bones. C3-mediated polarization of macrophages was responsible for the damage. CONCLUSION: T2DM-related upregulation of C3 contributes to the development of periodontitis by promoting macrophages M1 polarization and inhibiting M2 polarization, triggering a pro-inflammatory effect on periodontal tissues.


Assuntos
Complemento C3/metabolismo , Diabetes Mellitus Tipo 2/patologia , Macrófagos/imunologia , Periodontite/diagnóstico , Adulto , Processo Alveolar/diagnóstico por imagem , Processo Alveolar/patologia , Processo Alveolar/fisiologia , Animais , Densidade Óssea , Complemento C3/análise , Complemento C3/genética , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Experimental/patologia , Diabetes Mellitus Tipo 2/complicações , Feminino , Líquido do Sulco Gengival/metabolismo , Humanos , Interleucina-6/genética , Interleucina-6/metabolismo , Ativação de Macrófagos , Macrófagos/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Pessoa de Meia-Idade , Periodontite/etiologia , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/metabolismo
6.
PLoS One ; 15(8): e0237004, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32756589

RESUMO

Dysregulated expression of MicroRNAs (miRNAs) plays substantial role in the initiation and progression of both diabetes and periodontitis. The aim of the present study was to validate four miRNAs in saliva as potential predictive biomarkers of periodontal disease among patients with and without diabetes mellitus (DM). MiRNAs were extracted from the saliva of 24 adult subjects with DM and 29 healthy controls. Each group was subdivided into periodontally healthy or having periodontitis. In silico analysis identified 4 miRNAs (miRNA 155, 146 a/b and 203) as immune modulators. The expression of miRNAs-146a/b, 155, and 203 was tested using quantitative PCR. The expression levels in the study groups were compared to explore the effect of diabetes on periodontal status and vice versa. In our cohort, the four miRNAs expression were higher in patients with periodontitis and/or diabetes. miRNA-155 was the most reliable predictors of periodontitis among non-diabetics with an optimum cut-off value of < 8.97 with accuracy = 82.6%. MiRNA 146a, on the other hand, was the only reliable predictor of periodontitis among subjects with diabetes with optimum cut-off value of ≥11.04 with accuracy = 86.1%. The results of the present study concluded that MiRNA-146a and miRNA155 in saliva provide reliable, non-invasive, diagnostic and prognostic biomarkers that can be used to monitor periodontal health status among diabetic and non-diabetic patients.


Assuntos
Diabetes Mellitus/diagnóstico , MicroRNAs/metabolismo , Periodontite/diagnóstico , Adolescente , Adulto , Biomarcadores/metabolismo , Estudos de Casos e Controles , Complicações do Diabetes/diagnóstico , Complicações do Diabetes/genética , Complicações do Diabetes/metabolismo , Diabetes Mellitus/genética , Diabetes Mellitus/metabolismo , Análise Discriminante , Feminino , Humanos , Masculino , MicroRNAs/genética , Pessoa de Meia-Idade , Periodontite/genética , Periodontite/metabolismo , Projetos Piloto , Saliva/metabolismo , Regulação para Cima , Adulto Jovem
7.
High Blood Press Cardiovasc Prev ; 27(4): 281-289, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32500479

RESUMO

High blood pressure (BP) and periodontitis are two highly prevalent conditions worldwide with a significant impact on cardiovascular disease (CVD) complications. Poor periodontal health is associated with increased prevalence of hypertension and may have an influence on BP control. Risk factors such as older age, male gender, non-Caucasian ethnicity, smoking, overweight/obesity, diabetes, low socioeconomic status, and poor education have been considered the common denominators underpinning this relationship. However, recent evidence indicates that the association between periodontitis and hypertension is independent of common risk factors and may in fact be causal in nature. Low-grade systemic inflammation and redox imbalance, in particular, represent the major underlying mechanisms in this relationship. Neutrophil dysfunction, imbalance in T cell subtypes, oral-gut dysbiosis, hyperexpression of proinflammatory genes, and increased sympathetic outflow are some of the pathogenetic events involved. In addition, novel findings indicate that common genetic bases might shape the immune profile towards this clinical phenotype, offering a rationale for potential therapeutic and prevention strategies of public health interest. This review summarizes recent advances, knowledge gaps and possible future directions in the field.


Assuntos
Pressão Sanguínea , Hipertensão/epidemiologia , Periodontite/epidemiologia , Periodonto/microbiologia , Disbiose , Interações Hospedeiro-Patógeno , Humanos , Hipertensão/diagnóstico , Hipertensão/imunologia , Hipertensão/fisiopatologia , Periodontite/diagnóstico , Periodontite/imunologia , Periodontite/microbiologia , Prevalência , Prognóstico , Medição de Risco , Fatores de Risco
8.
Acta Odontol Scand ; 78(7): 553-559, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32552160

RESUMO

Background: Periodontitis has been associated with several systemic diseases and medical conditions, including oral cancer (OC). However, most studies reporting an association between OC and periodontal disease have used different clinical and radiographic criteria to define periodontal disease. This review aimed to evaluate the currently available evidence to determine an association between periodontal disease (extension and severity), OC, and oral potentially malignant disorders (OPMDs).Material and methods: A systematic search of studies published up to August 2018 was performed following the PRISMA guidelines in the electronic databases MEDLINE (PubMed) and COCHRANE (OVID). A methodological evaluation was made using the Strengthening the Reporting of Observational studies in Epidemiology (STROBE) checklist.Results: Eight studies (case-control, cross-sectional and cohort) were included. An increased clinical attachment loss, plaque index, bleeding on probing, and radiographic bone loss was found in patients with OC and OPMDs. Differences in the methodological characteristics, case definition used for periodontal diseases, and OC location did not allow estimating the odds ratio required to conduct a meta-analysis.Conclusion: Some studies suggest a positive relationship between periodontal disease, OC, and OPMDs; however, the currently available evidence is insufficient to draw solid conclusions.


Assuntos
Neoplasias Bucais , Doenças Periodontais , Periodontite , Estudos de Casos e Controles , Estudos Transversais , Humanos , Neoplasias Bucais/complicações , Neoplasias Bucais/diagnóstico , Neoplasias Bucais/epidemiologia , Doenças Periodontais/complicações , Doenças Periodontais/diagnóstico , Doenças Periodontais/epidemiologia , Periodontite/complicações , Periodontite/diagnóstico , Periodontite/epidemiologia
9.
Sci Rep ; 10(1): 7093, 2020 04 27.
Artigo em Inglês | MEDLINE | ID: mdl-32341429

RESUMO

We aimed to compare the accuracy performance of the new 2018 periodontitis case definition by the European Federation of Periodontology (EFP)/ American Association of Periodontology (AAP) with Centers for Disease Control (CDC)/AAP 2012 in full-mouth partial recording protocols (PRP). Retrospective data from NHANES 2011-2012 and 2013-2014 were analyzed. For each case definition, full-mouth diagnostic was defined as the reference standard. Patients were diagnosed for the presence of periodontitis and staging for each PRP. Sensitivity, specificity, accuracy and precision, through several indicators, were determined. Performance measurement was assessed through binary and multiclass ROC/AUC analyses. Our performance analysis shows that the new 2018 classification outperforms the 2012 classification regarding the diagnosis and staging of periodontitis on full-mouth PRPs. This recent case definition has strengthened the utility of PRPs and its improvements certainly explain the observed findings. Also, our findings contribute to the reliability of PRPs and its use in future worldwide epidemiological surveys.


Assuntos
Periodontite/classificação , Periodontite/diagnóstico , Periodontite/epidemiologia , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
10.
Braz Oral Res ; 34(supp1 1): e027, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32294680

RESUMO

Periodontal diseases are considered a worldwide public health problem, owing to their high prevalence in developed and developing countries. Periodontitis may lead to tooth loss, which can impact oral health-related quality of life. Gingivitis and periodontitis have been extensively studied regarding their etiopathogenesis, epidemiology, prevention and treatment outcomes. However, most of these aspects are studied and discussed globally, which may hamper a clear interpretation of the findings and the design of effective plans of action for specific regions or populations. For example, in Latin America, epidemiological data about the distribution of periodontal diseases is still scarce, mainly when it comes to nationwide representative samples. This Consensus aimed to address the following topics related to periodontal diseases in Latin America: a) The impact of the global burden of periodontal diseases on health: a global reality; b) Periodontal diseases in Latin America; c) Strategies for the prevention of periodontal diseases in Latin America; d) Problems associated with diagnosis of periodontal conditions and possible solutions for Latin America; e) Treatment of Periodontitis. This consensus will help to increase awareness about diagnosis, prevention and treatment of periodontal diseases, in the context of Latin American countries.


Assuntos
Conferências de Consenso como Assunto , Doenças Periodontais/terapia , Feminino , Gengivite/diagnóstico , Gengivite/epidemiologia , Gengivite/terapia , Carga Global da Doença , Humanos , América Latina/epidemiologia , Masculino , Saúde Bucal , Doenças Periodontais/diagnóstico , Doenças Periodontais/epidemiologia , Periodontite/diagnóstico , Periodontite/epidemiologia , Periodontite/terapia , Qualidade de Vida
11.
PLoS One ; 15(3): e0230334, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32168352

RESUMO

AIM: The aim of the present study was to assess the feasibility and diagnostic contribution of protein profiling using MALDI-TOF mass spectrometry applied to saliva, gingival crevicular fluid (GCF) and dental plaque from periodontitis and healthy subjects. We hypothesized that rapid routine and blinded MALDI-TOF analysis could accurately classify these three types of samples according to periodontal state. MATERIALS AND METHODS: Unstimulated saliva, GCF and dental plaque, collected from periodontitis subjects and healthy controls, were analyzed by MALDI-TOF MS. Based on the differentially expressed peaks between the two groups, diagnostic decision trees were built for each sample. RESULTS: Among 141 patients (67 periodontitis and 74 healthy controls), the decision trees diagnosed periodontitis with a sensitivity = 70.3% (± 0.211) and a specificity = 77.8% (± 0.165) for saliva, a sensitivity = 79.6% (± 0.188) and a specificity = 75.7% (± 0.195) for GCF, and a sensitivity = 72.1% (± 0.202) and a specificity = 72.2% (± 0.195) for dental plaque. The sensitivity and specificity of the tests were improved to 100% (CI 95% = [0.91;1]) and 100% (CI 95% = [0.92;1]), respectively, when two samples were tested. CONCLUSION: We developed, for the first time, diagnostic tests based on protein profiles of saliva, GCF and dental plaque between periodontitis patients and healthy subjects. When at least 2 of these samples were tested, the best results were obtained.


Assuntos
Periodontite/diagnóstico , Proteínas/genética , Saliva/metabolismo , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Adulto , Feminino , Regulação da Expressão Gênica/genética , Humanos , Masculino , Pessoa de Meia-Idade , Periodontite/genética , Periodontite/patologia , Proteínas/isolamento & purificação , Dente/metabolismo , Dente/patologia
12.
Klin Lab Diagn ; 65(1): 55-60, 2020.
Artigo em Russo | MEDLINE | ID: mdl-32155008

RESUMO

Inflammatory periodontal diseases represent a serious dental and general medical problem due to the high prevalence among the adult population, the presence of clinical forms leading to the destruction of the dentition and tooth loss, insufficient treatment effectiveness and the frequency of relapse, including in connection with the formation of biofilms. A molecular genetic test system has been developed to evaluate the content of periodontopathogenic microorganisms Porphyromonas gingivalis, Treponema denticola, Streptococcus oralis, Streptococcus sanguis and Streptococcus sobrinus in the contents of periodontal pockets. The analytical characteristics of the test system were determined, and testing was carried out on clinical samples of patients with chronic generalized periodontitis of moderate severity. The constructed diagnostic kit allowed us to conduct a comparative analysis of the effectiveness of various types of treatment of inflammatory periodontal diseases based on quantitative data on the content of bacteria in the contents of periodontal pockets.


Assuntos
Bolsa Periodontal/microbiologia , Periodontite/diagnóstico , Periodontite/microbiologia , Adulto , Aggregatibacter actinomycetemcomitans , Bacteroides/isolamento & purificação , Diagnóstico Precoce , Testes Genéticos , Humanos , Porphyromonas gingivalis/isolamento & purificação , Streptococcus oralis/isolamento & purificação , Streptococcus sanguis/isolamento & purificação , Streptococcus sobrinus/isolamento & purificação , Treponema denticola/isolamento & purificação
13.
Sci Rep ; 10(1): 1402, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31996751

RESUMO

The aim of this prospective cohort study was to investigate the association between intrauterine fetal growth patterns and periodontal status in pregnant women. Fifty-three pregnant women were recruited. Periodontitis was diagnosed based on probing pocket depth and clinical attachment level. Maternal urinary 8-hydroxy-2'-deoxyguanosine levels and body mass index were recorded. Ultrasonographic measurements of the biparietal diameter (BPD), abdominal circumference (AC), and femur length (FL) were recorded, and estimated fetal weight (EFW) was calculated. In addition, approximation spline curves of BPD, AC, FL, and EFW were obtained throughout the gestation period. Recorded delivery outcomes were gestational age (GA), birth weight and length, sex, placental weight, and umbilical cord length. Forty-four participants (34.1 ± 4.9 years) were analyzed. Mean neonatal birth weight was 2906.0 ± 544.4 g. On multiple regression analysis, birth weight was related with periodontitis (standardized ß = -0.21, P = 0.001). For EFW and BPD, the curves of the periodontitis group were located lower than those of the non-periodontitis group, with significant differences after 32 weeks and 20 weeks of GA, respectively. In conclusion, periodontal treatment before conception may be recommended and a good periodontal condition in the early stage of pregnancy at the latest is desirable for infant growth.


Assuntos
Desenvolvimento Fetal/fisiologia , Saúde Materna , Periodontite/fisiopatologia , Complicações na Gravidez/fisiopatologia , Adulto , Peso ao Nascer/fisiologia , Feminino , Feto/diagnóstico por imagem , Feto/fisiologia , Humanos , Recém-Nascido de Baixo Peso , Recém-Nascido , Estudos Longitudinais , Índice Periodontal , Periodontite/diagnóstico , Gravidez , Complicações na Gravidez/diagnóstico , Estudos Prospectivos , Ultrassonografia Pré-Natal
14.
Int J Oral Sci ; 12(1): 2, 2020 01 02.
Artigo em Inglês | MEDLINE | ID: mdl-31900383

RESUMO

Interleukin(IL)-1ß, a pro-inflammatory cytokine, was elevated and participates in periodontitis. Not only the link between IL-1ß and periodontitis was proved by clinical evidence, but also the increased IL-1ß triggers a series of inflammatory reactions and promotes bone resorption. Currently, IL-1ß blockage has been therapeutic strategies for autoimmune and autoinflammatory diseases such as rheumatoid arthritis, cryopyrin-associated periodic syndromes, gout and type II diabetes mellitus. It is speculated that IL-1ß be a potential therapeutic target for periodontitis. The review focuses on the production, mechanism, present treatments and future potential strategies for IL-1ß in periodontitis.


Assuntos
Inflamação/terapia , Interleucina-1beta/antagonistas & inibidores , Interleucina-1beta/imunologia , Periodontite/terapia , Citocinas , Diabetes Mellitus Tipo 2 , Humanos , Inflamação/imunologia , Interleucina-1beta/efeitos dos fármacos , Periodontite/diagnóstico
15.
Anaerobe ; 61: 102089, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31430531

RESUMO

Cardamom (Elettaria cardamomum) is a traditional aromatic plant for which several pharmacological properties have been associated. In this study, the antibacterial activity of two cardamom extracts (fruit and seeds), rich in volatile compounds, against major periodontal pathogens was evaluated. Moreover, the ability of the extracts to exert anti-inflammatory activity was tested. Both cardamom fruit and seed extracts exerted an antibacterial effect against Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, and Prevotella intermedia (minimum inhibitory concentrations: 0.5% [v/v], 0.25%, 0.062%, 0.125%, respectively and minimum bactericidal concentrations: 1%, 0.25%, 0.062%, 0.25%, respectively). The cell membrane of P. gingivalis was disrupted by a treatment with cardamom extracts suggesting the bactericidal mode of action. The extracts also inhibited biofilm formation although it correlated with a growth reduction. Moreover, the cardamom extracts significantly decreased the secretion of IL-1ß, TNF-α, and IL-8 by lipopolysaccharide-stimulated macrophages. Evidence were brought that the anti-inflammatory activity may result from inhibition of the NF-κB signaling pathway. This study is the first to provide evidence that cardamom fruit and seed extracts through their antibacterial and anti-inflammatory properties may be therapeutic agents of interest against periodontal infections.


Assuntos
Antibacterianos/farmacologia , Anti-Inflamatórios/farmacologia , Elettaria/química , Extratos Vegetais/farmacologia , Antibacterianos/química , Anti-Inflamatórios/química , Biofilmes/efeitos dos fármacos , Permeabilidade da Membrana Celular , Biologia Computacional/métodos , Citocinas/metabolismo , Humanos , Lipopolissacarídeos/imunologia , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Macrófagos/metabolismo , Testes de Sensibilidade Microbiana , NF-kappa B/metabolismo , Periodontite/diagnóstico , Periodontite/tratamento farmacológico , Periodontite/microbiologia , Extratos Vegetais/química
16.
Cardiovasc Res ; 116(1): 28-39, 2020 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-31549149

RESUMO

Recent evidence suggests a link between periodontitis (PD) and hypertension, but the nature of this association remains unclear. The overall aim of this review was to critically appraise the evidence linking these two common disorders. Systematic search was conducted for studies published up to December 2018. Prevalence of hypertension in patients with PD (moderate/severe groups) vs. those without PD (non-PD) was the primary outcome. Additional outcomes included adjusted mean difference in systolic (SBP) and diastolic (DBP) blood pressure (BP) levels in PD vs. non-PD, assessment of biomarkers in PD and hypertension, and BP changes after periodontal therapy. From 81 studies selected, 40 were included in quantitative meta-analyses. Diagnoses of moderate-severe PD [odds ratio (OR) = 1.22; 95% confidence interval (CI): 1.10-1.35] and severe PD (OR = 1.49; 95% CI: 1.09-2.05) were associated with hypertension. Prospective studies confirmed PD diagnosis increased likelihood of hypertension occurrence (OR = 1.68; 95% CI: 0.85-3.35). Patients with PD exhibited higher mean SBP [weighted mean difference (WMD) of 4.49 mmHg; 95% CI: 2.88-6.11] and DBP (2.03 mmHg; 95% CI: 1.25-2.81) when compared with non-PD. Lastly, only 5 out of 12 interventional studies confirmed a reduction in BP following periodontal therapy, ranging from 3 to 12.5 mmHg of SBP and from 0 to 10 mmHg of DBP. PD is associated with increased odds of hypertension (SORT C) and higher SBP/DBP levels. The evidence suggesting that PD therapy could reduce BP is inconclusive. Although additional research is warranted on this association, these results suggest that oral health assessment and management of PD could not only improve oral/overall health and quality of life but also be of relevance in the management of patients with hypertension.


Assuntos
Pressão Sanguínea , Hipertensão/epidemiologia , Saúde Bucal , Periodontite/epidemiologia , Assistência Odontológica , Feminino , Humanos , Hipertensão/diagnóstico , Hipertensão/fisiopatologia , Hipertensão/prevenção & controle , Masculino , Higiene Bucal , Periodontite/diagnóstico , Periodontite/terapia , Prevalência , Prognóstico , Qualidade de Vida , Medição de Risco , Fatores de Risco , Índice de Gravidade de Doença
17.
J Clin Periodontol ; 47(2): 182-192, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31680280

RESUMO

AIMS: The present study aimed to monitor the levels of selected salivary biomarkers during the development and treatment of periodontitis and to evaluate their ability to identify periodontitis in dogs. MATERIALS AND METHODS: A total of 15 beagle dogs were divided into a control group (no ligature), group 1 (ligature on six teeth), and group 2 (ligature on 12 teeth). The experimental periods consisted of 8 weeks of periodontitis induction and 4 weeks of treatment. Clinical measurements and the sampling of saliva were performed every 4 weeks. The levels of S100A8, S100A9, S100A8/A9, and matrix metalloproteinase (MMP)-9 were measured by enzyme-linked immunosorbent assay. RESULTS: All experimental animals and two control animals developed periodontitis, which was successfully treated. All salivary biomarkers were significantly increased in periodontitis with high diagnostic power (c-index ≥ 0.944) and were able to identify animals with periodontitis on a single tooth. Whereas the levels of salivary S100A8/A9 recovered to levels in health, those of S100A8, S100A9, and MMP-9 in periodontitis stability remained significantly higher than in health. CONCLUSION: Salivary S100A8, S100A9, S100A8/A9, and MMP-9 may be used for the screening of periodontitis in dogs, but with caution of other conditions that can affect their levels in saliva.


Assuntos
Metaloproteinase 9 da Matriz , Periodontite/diagnóstico , Animais , Calgranulina A , Calgranulina B , Cães , Metaloproteinase 8 da Matriz , Proteínas S100
18.
Acta Odontol Scand ; 78(4): 290-296, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31852321

RESUMO

Objectives: The mechanisms underlying the formation and composition of gingival crevicular fluid (GCF) and its flow into and from periodontal pockets are not understood very well. The aim of this study was to evaluate the length of sampling time and sequential sampling of GCF neutrophil elastase (NE) enzyme levels by using intracrevicular and orifice methods.Material and methods: Twenty adults (mean age of 41.8 years, ranged 31-60 years, 18 males and 2 females) with chronic periodontitis were enrolled and all completed the 3-d study. GCF was collected by both intracrevicular and intrasulcular methods, 720 samples of GCF were collected. In first, second and third day, the length of sampling time in seconds (s) and order were '5- 10-30-s'; '10- 30- 5-s' and '30- 5- 10-s,' respectively. GCF elastase levels were determined by hydrolysis of neutrophil specific substrate N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide.Results: NE activity (µU) and NE activity/volume (µU/µl) were significantly different for order of sampling (p < .05), but not for the length of sampling time (p>.05).Conclusions: Within the limits of this study, the choice of sampling technique in GCF-profile studies seems to be a critical decision as it has the potential to affect the GCF volume and NE activity.


Assuntos
Líquido do Sulco Gengival/química , Gengivite/enzimologia , Elastase de Leucócito/metabolismo , Periodontite/enzimologia , Adulto , Feminino , Bolsa Gengival/enzimologia , Gengivite/diagnóstico , Humanos , Masculino , Pessoa de Meia-Idade , Índice Periodontal , Periodontite/diagnóstico , Fatores de Tempo
19.
Immunol Lett ; 218: 11-21, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31863783

RESUMO

Aging humans display an increased prevalence and severity of periodontitis, although the mechanisms underlying these findings remain poorly understood. This report examined antigenic diversity of P. gingivalis related to disease presence and patient demographics. Serum IgG antibody to P. gingivalis strains ATCC33277, FDC381, W50 (ATCC53978), W83, A7A1-28 (ATCC53977) and A7436 was measured in 426 participants [periodontally healthy (n = 61), gingivitis (N = 66) or various levels of periodontitis (N = 299)]. We hypothesized that antigenic diversity in P. gingivalis could contribute to a lack of "immunity" in the chronic infections of periodontal disease. Across the strains, the antibody levels in the oldest age group were lower than in the youngest groups, and severe periodontitis patients did not show higher antibody with aging. While 80 % of the periodontitis patients in any age group showed an elevated response to at least one of the P. gingivalis strains, the patterns of individual responses in the older group were also substantially different than the other age groups. Significantly greater numbers of older patients showed strain-specific antibody profiles to only 1 strain. The findings support that P. gingivalis may demonstrate antigenic diversity/drift within patients and could be one factor to help explain the inefficiency/ineffectiveness of the adaptive immune response in managing the infection.


Assuntos
Anticorpos Antibacterianos/imunologia , Infecções por Bacteroidaceae/diagnóstico , Infecções por Bacteroidaceae/imunologia , Variação Biológica Individual , Periodontite/diagnóstico , Periodontite/etiologia , Porphyromonas gingivalis/imunologia , Adulto , Idoso , Anticorpos Antibacterianos/sangue , Infecções por Bacteroidaceae/microbiologia , Biomarcadores , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Vigilância da População , Adulto Jovem
20.
J Periodontal Res ; 55(2): 267-276, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31769036

RESUMO

OBJECTIVES: The aim of this present study was to evaluate the levels of Netrin-1 and Unc5b in periodontal health and disease. BACKGROUND: Netrin-1, acting via its receptor UNC5b, regulates the inflammatory response and takes apart in bone destructive diseases. METHODS: Samples of gingival crevicular fluid (GCF), whole saliva, and serum were taken from systemically healthy, nonsmoking 20 periodontitis , 20 gingivitis patients, and 20 periodontally healthy subjects at baseline and 4 weeks after nonsurgical periodontal treatment (NSPT). Whole-mouth and site-specific clinical periodontal parameters were recorded. Netrin-1 and Unc5b levels were measured by enzyme-linked immunosorbent assay. Data were analyzed by nonparametric tests. RESULTS: Total amount of Netrin-1 in GCF was significantly higher in periodontitis than the others, and the levels were significantly reduced after NSPT. Salivary and serum concentrations of Netrin-1 were significantly different among the study groups (P = .000), and NSPT significantly increased the concentration levels of both salivary and serum Netrin-1 (P < .05). Healthy subjects had significantly lower GCF (P = .001) and conversely, higher salivary and serum levels of Unc5b than the other groups (P = .002). The GCF levels of Unc5b were significantly reduced (P < .01), and conversely, serum concentrations were significantly increased after NSPT (P < .05). GCF Netrin-1 and Unc5b total amounts were positively correlated with clinical parameters (P < .01 and P < .05) whereas salivary Netrin-1 and Unc5b concentrations were negatively correlated with clinical parameters (P < .01 and P < .05). CONCLUSIONS: The results of this study indicate that Netrin-1 and its receptor Unc5b may have essential roles in periodontal inflammation and those can be assumed as useful therapeutic agent to control inflammation and periodontal breakdown.


Assuntos
Receptores de Netrina/análise , Netrina-1/análise , Periodontite/diagnóstico , Estudos de Casos e Controles , Líquido do Sulco Gengival/química , Gengivite , Humanos , Receptores de Superfície Celular , Saliva/química , Soro/química
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