Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 4.183
Filtrar
1.
Av. odontoestomatol ; 36(3): 143-149, sept.-dic. 2020.
Artigo em Espanhol | IBECS | ID: ibc-197413

RESUMO

Los productos naturales son utilizados ancestralmente en la medicina tradicional, también llamados medicamentos herbarios, que vienen siendo utilizados por sus múltiples propiedades curativas y ante la necesidad de obtener nuevos productos que ayuden o apoyen al tratamiento de múltiples enfermedades, son en la actualidad una alternativa. La Organización Mundial de la Salud define un medicamento herbario como un preparado, producto herbario que contenga el principio activo en una parte de la planta. En la actualidad en la región amazónica de América del Sur, se vienen obteniendo una oleorresina extraída de un árbol llamado copaiba, con propiedades antimicrobianas que tiene una proyección en su uso en patologías periodontales como la periodontitis, patología cuyo mayor factor etiológico es la presencia de una biopelícula microbiana en el surco periodontal, que va degradando progresivamente los tejidos de soporte del diente, pudiendo generar la perdida de la pieza dentaria


Natural products are used anciently in traditional medicine, also called herbal medicines, which have been used for their multiple healing properties and, given the need to obtain new products that help or support the treatment of multiple diseases, are currently an alternative. The World Health Organization defines an herbal medicine as a preparation, herbal product that contains the active substance in a part of the plant. At present in the Amazon region of South America, an oleoresin extracted from a tree called copaiba is being obtained, with antimicrobial properties that has a projection in its use in periodontal pathologies such as periodontitis, pathology whose major etiological factor is the presence of a microbial biofilm in the periodontal groove, which gradually degrades the supporting tissues of the tooth, and can lead to the loss of the tooth


Assuntos
Humanos , Periodontite/terapia , Produtos Biológicos/uso terapêutico , Medicina Tradicional/métodos , Fabaceae , Periodontite/microbiologia , Extratos Vegetais/metabolismo , Antibacterianos/uso terapêutico , Boca/efeitos dos fármacos , Boca/microbiologia
2.
J Appl Oral Sci ; 28: e20200501, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33331391

RESUMO

OBJECTIVE: This study aimed to clarify the association between oral human cytomegalovirus (HCMV) and periodontitis in Japanese adults. METHODOLOGY: In total, 190 patients (75 men and 115 women; mean age, 70.2 years) who visited Hiroshima University Hospital between March 2018 and May 2020 were included. Oral rinse samples were taken to examine the presence of HCMV DNA using real-time polymerase chain reaction (PCR). P. gingivalis was detected by semi-quantitative PCR analysis. RESULTS: HCMV DNA was present in nine of 190 patients (4.7%). There were significant associations between HCMV presence and the presence of ≥4-mm-deep periodontal pockets with bleeding on probing (BOP) (P<0.01) and ≥6-mm-deep periodontal pockets with BOP (P=0.01). However, no significant relationship was observed between HCMV presence and periodontal epithelial surface area scores. Logistic regression analysis revealed that the presence of ≥4-mm-deep periodontal pockets with BOP was significantly associated with HCMV (odds ratio, 14.4; P=0.01). Propensity score matching was performed between patients presenting ≥4-mm-deep periodontal pockets with BOP (i.e., active periodontitis) and patients without ≥4-mm-deep periodontal pockets with BOP; 62 matched pairs were generated. Patients who had ≥4-mm-deep periodontal pockets with BOP showed a higher rate of HCMV presence (9.7%) than those who lacked ≥4-mm-deep periodontal pockets with BOP (0.0%). There was a significant relationship between HCMV presence and ≥4-mm-deep periodontal pockets with BOP (P=0.03). A significant relationship was found between HCMV/P. gingivalis DNA presence and ≥4-mm-deep periodontal pockets with BOP (P=0.03). CONCLUSIONS: Coinfection of oral HCMV and P. gingivalis was significantly associated with active periodontitis. Moreover, interactions between oral HCMV and P. gingivalis may be related to the severity of periodontal disease.


Assuntos
Infecções por Bacteroidaceae/epidemiologia , Infecções por Citomegalovirus/epidemiologia , Periodontite , Idoso , Coinfecção , Estudos Transversais , Citomegalovirus , Feminino , Humanos , Japão/epidemiologia , Masculino , Bolsa Periodontal/microbiologia , Bolsa Periodontal/virologia , Periodontite/epidemiologia , Periodontite/microbiologia , Periodontite/virologia , Porphyromonas gingivalis , Prevalência
3.
PLoS One ; 15(12): e0242868, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33382721

RESUMO

Rheumatoid arthritis (RA) and periodontitis (PD) are chronic inflammatory diseases that appear to occur in tandem. However, the mutual impact PD exerts on RA and vice versa has not yet been defined. To address this issue, we set up an animal model and analyzed how two prime inducers of periodontitis-Porphyromonas gingivalis (Pg) and Aggregatibacter actinomycetemcomitans (Aa)-differ in their pathogenic potential. Our experimental setup included collagen induced arthritis (CIA) in the mouse, oral inoculation with Pg or Aa to induce alveolar bone loss and the combination of both diseases in inverted orders of events. Neither pathobiont impacted on macroscopic arthritis and arthritis did not exacerbate alveolar bone loss. However, there were subtle differences between Pg and Aa with the former inducing more alveolar bone loss if PD was induced before CIA. On a molecular level, Pg and Aa led to differential expression patterns in the synovial membranes that were reminiscent of cellular and humoral immune responses, respectively. The Pg and Aa specific signatures in the synovial proteomes suggest a role for oral pathogens in shaping disease subtypes and setting the stage for subsequent therapy response.


Assuntos
Aggregatibacter actinomycetemcomitans/fisiologia , Artrite Reumatoide/metabolismo , Artrite Reumatoide/microbiologia , Porphyromonas gingivalis/fisiologia , Proteoma/metabolismo , Membrana Sinovial/metabolismo , Animais , Citocinas/metabolismo , Camundongos , Periodontite/microbiologia , Membrana Sinovial/microbiologia
4.
Int J Nanomedicine ; 15: 5473-5489, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32801701

RESUMO

Introduction: Biofilms protect bacteria from antibiotics and this can produce drug-resistant strains, especially the main pathogen of periodontitis, Porphyromonas gingivalis. Carbon quantum dots with various biomedical properties are considered to have great application potential in antibacterial and anti-biofilm treatment. Methods: Tinidazole carbon quantum dots (TCDs) and metronidazole carbon quantum dots (MCDs) were prepared by a hydrothermal method with the clinical antibacterial drugs tinidazole and metronidazole, respectively. Then, TCDs and MCDs were characterized by transmission electron microscopy, UV-visible spectroscopy, infrared spectroscopy and energy-dispersive spectrometry. The antibacterial effects were also investigated under different conditions. Results: The TCDs and MCDs had uniform sizes. The results of UV-visible and energy-dispersive spectrometry confirmed their important carbon polymerization structures and the activity of the nitro group, which had an evident inhibitory effect on P. gingivalis, but almost no effect on other bacteria, including Escherichia coli, Staphylococcus aureus and Prevotella nigrescens. Importantly, the TCDs could penetrate the biofilms to further effectively inhibit the growth of P. gingivalis under the biofilms. Furthermore, it was found that the antibacterial effect of TCDs lies in its ability to impair toxicity by inhibiting the major virulence factors and related genes involved in the biofilm formation of P. gingivalis, thus affecting the self-assembly of biofilm-related proteins. Conclusion: The findings demonstrate a promising new method for improving the efficiency of periodontitis treatment by penetrating the P. gingivalis biofilm with preparations of nano-level antibacterial drugs.


Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Porphyromonas gingivalis/efeitos dos fármacos , Pontos Quânticos/química , Animais , Antibacterianos/efeitos adversos , Aderência Bacteriana/efeitos dos fármacos , Biofilmes/efeitos dos fármacos , Carbono/química , Carbono/farmacologia , Escherichia coli/efeitos dos fármacos , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Humanos , Metronidazol/química , Metronidazol/farmacologia , Testes de Sensibilidade Microbiana , Microscopia Eletrônica de Transmissão , Periodontite/microbiologia , Porphyromonas gingivalis/genética , Porphyromonas gingivalis/fisiologia , Coelhos , Espectrofotometria Ultravioleta , Staphylococcus aureus/efeitos dos fármacos , Tinidazol/química , Tinidazol/farmacologia , Fatores de Virulência/antagonistas & inibidores
5.
High Blood Press Cardiovasc Prev ; 27(4): 281-289, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32500479

RESUMO

High blood pressure (BP) and periodontitis are two highly prevalent conditions worldwide with a significant impact on cardiovascular disease (CVD) complications. Poor periodontal health is associated with increased prevalence of hypertension and may have an influence on BP control. Risk factors such as older age, male gender, non-Caucasian ethnicity, smoking, overweight/obesity, diabetes, low socioeconomic status, and poor education have been considered the common denominators underpinning this relationship. However, recent evidence indicates that the association between periodontitis and hypertension is independent of common risk factors and may in fact be causal in nature. Low-grade systemic inflammation and redox imbalance, in particular, represent the major underlying mechanisms in this relationship. Neutrophil dysfunction, imbalance in T cell subtypes, oral-gut dysbiosis, hyperexpression of proinflammatory genes, and increased sympathetic outflow are some of the pathogenetic events involved. In addition, novel findings indicate that common genetic bases might shape the immune profile towards this clinical phenotype, offering a rationale for potential therapeutic and prevention strategies of public health interest. This review summarizes recent advances, knowledge gaps and possible future directions in the field.


Assuntos
Pressão Sanguínea , Hipertensão/epidemiologia , Periodontite/epidemiologia , Periodonto/microbiologia , Disbiose , Interações Hospedeiro-Patógeno , Humanos , Hipertensão/diagnóstico , Hipertensão/imunologia , Hipertensão/fisiopatologia , Periodontite/diagnóstico , Periodontite/imunologia , Periodontite/microbiologia , Prevalência , Prognóstico , Medição de Risco , Fatores de Risco
6.
Arch Oral Biol ; 115: 104742, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32416352

RESUMO

BACKGROUND AND OBJECTIVE: Periodontitis is an oral chronic inflammatory disease caused by dental plaque. It is comorbid with numerous systemic diseases and associated with several predisposing factors, such as chronic kidney disease (CKD). Peritoneal dialysis is one of the ultimate treatments for patients with severe CKD. However, peritoneal dialysis patients with periodontitis often will be accompanied with more poor oral hygiene and periodontal clinical indexes. This study aimed to compare the microbial flora of periodontitis patients with or without peritoneal dialysis. METHODS: Sixteen peritoneal dialysis patients with periodontitis (P group) and 16 patients with periodontitis only (C group) were selected. Subgingival plaque samples of them were processed for bioinformatics analysis by 16S rDNA gene sequencing. RESULTS: The diversity indices and species richness in the P group were insignificantly higher than that in the C group (P > 0.05). The two groups exhibited different microbial community structure. At Genus level, Prevotellaceae, Selenomonas, Aggregatibacter, Anaeroglobus, TM7_[G-5], and Centipeda were significantly enriched in the P group than those in the C group. CONCLUSIONS: This study demonstrated that specific microbes enriched in the subgingival flora of peritoneal dialysis patients with periodontitis.


Assuntos
Microbiota , Periodontite , Diálise Peritoneal , Bactérias/genética , Gengiva/microbiologia , Humanos , Periodontite/microbiologia , RNA Ribossômico 16S/genética
7.
Sci Rep ; 10(1): 7823, 2020 05 08.
Artigo em Inglês | MEDLINE | ID: mdl-32385413

RESUMO

This study investigates the role of NLRP3 inflammasome and its main effector Caspase-1 in inflammation and alveolar bone resorption associated with periodontitis. Heat-killed Aggregatibacter actinomycetemcomitans (Aa) was injected 3x/week (4 weeks) into gingival tissues of wild-type (WT), Nlrp3-KO and Caspase1-KO mice. Bone resorption was measured by µCT and osteoclast number was determined by tartrate-resistant acid phosphatase (TRAP) staining. Inflammation was assessed histologically (H/E staining and immunofluorescence of CD45 and Ly6G). In vitro studies determined the influence of Nlrp3 and Caspase-1 in Rankl-induced osteoclast differentiation and activity and on LPS-induced expression of inflammation-associated genes. Bone resorption was significantly reduced in Casp1-KO but not in Nlrp3-KO mice. Casp1-KO mice had increased in osteoclast numbers, whereas the inflammatory infiltrate or on gene expression were similar to those of WT and Nlrp3-KO mice. Strikingly, osteoclasts differentiated from Nlrp3-deficient macrophages had increased resorbing activity in vitro. LPS-induced expression of Il-10, Il-12 and Tnf-α was significantly reduced in Nlrp3- and Casp1-deficient macrophages. As an inceptive study, these results suggest that Nlrp3 inflammasome does not play a significant role in inflammation and bone resorption in vivo and that Caspase-1 has a pro-resorptive role in experimental periodontal disease.


Assuntos
Perda do Osso Alveolar/genética , Caspase 1/genética , Inflamação/genética , Proteína 3 que Contém Domínio de Pirina da Família NLR/genética , Periodontite/genética , Aggregatibacter actinomycetemcomitans , Perda do Osso Alveolar/microbiologia , Perda do Osso Alveolar/patologia , Animais , Diferenciação Celular/efeitos dos fármacos , Modelos Animais de Doenças , Gengiva/crescimento & desenvolvimento , Gengiva/microbiologia , Humanos , Inflamação/microbiologia , Inflamação/patologia , Interleucina-10/genética , Interleucina-12/genética , Camundongos , Camundongos Knockout , Osteoclastos/microbiologia , Osteoclastos/patologia , Periodontite/microbiologia , Periodontite/patologia , Ligante RANK/genética , Fator de Necrose Tumoral alfa/genética
8.
PLoS One ; 15(5): e0232912, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32392236

RESUMO

The study of oral disease progression, in relation to the accumulation of subgingival biofilm in gingivitis and periodontitis is limited, due to either the ability to monitor plaque in vitro. When compared, optical spectroscopic techniques offer advantages over traditional destructive or biofilm staining approaches, making it a suitable alternative for the analysis and continued development of three-dimensional structures. In this work, we have developed a confocal Raman spectroscopy analysis approach towards in vitro subgingival plaque models. The main objective of this study was to develop a method for differentiating multiple oral subgingival bacterial species in planktonic and biofilm conditions, using confocal Raman microscopy. Five common subgingival bacteria (Fusobacterium nucleatum, Streptococcus mutans, Veillonella dispar, Actinomyces naeslundii and Prevotella nigrescens) were used and differentiated using a 2-way orthogonal Partial Least Square with Discriminant Analysis (O2PLS-DA) for the collected spectral data. In addition to planktonic growth, mono-species biofilms cultured using the 'Zürich Model' were also analyzed. The developed method was successfully used to predict planktonic and mono-species biofilm species in a cross validation setup. The results show differences in the presence and absence of chemical bands within the Raman spectra. The O2PLS-DA model was able to successfully predict 100% of all tested planktonic samples and 90% of all mono-species biofilm samples. Using this approach we have shown that Confocal Raman microscopy can analyse and predict the identity of planktonic and mono-species biofilm species, thus enabling its potential as a technique to map oral multi-species biofilm models.


Assuntos
Bactérias/isolamento & purificação , Gengivite/microbiologia , Microscopia Óptica não Linear/métodos , Periodontite/microbiologia , Actinomyces , Técnicas Bacteriológicas/métodos , Biofilmes/crescimento & desenvolvimento , Meios de Cultura , Fusobacterium nucleatum , Gengiva/microbiologia , Viabilidade Microbiana , Microbiota , Microscopia Confocal/métodos , Plâncton , Prevotella intermedia , Streptococcus mutans , Veillonella
9.
Nat Microbiol ; 5(8): 1016-1025, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32393857

RESUMO

Porphyromonas gingivalis, an asaccharolytic member of the Bacteroidetes, is a keystone pathogen in human periodontitis that may also contribute to the development of other chronic inflammatory diseases. P. gingivalis utilizes protease-generated peptides derived from extracellular proteins for growth, but how these peptides enter the cell is not clear. Here, we identify RagAB as the outer-membrane importer for these peptides. X-ray crystal structures show that the transporter forms a dimeric RagA2B2 complex, with the RagB substrate-binding surface-anchored lipoprotein forming a closed lid on the RagA TonB-dependent transporter. Cryo-electron microscopy structures reveal the opening of the RagB lid and thus provide direct evidence for a 'pedal bin' mechanism of nutrient uptake. Together with mutagenesis, peptide-binding studies and RagAB peptidomics, our work identifies RagAB as a dynamic, selective outer-membrane oligopeptide-acquisition machine that is essential for the efficient utilization of proteinaceous nutrients by P. gingivalis.


Assuntos
Proteínas de Bactérias/metabolismo , Proteínas de Membrana Transportadoras/metabolismo , Oligopeptídeos/metabolismo , Porphyromonas gingivalis/metabolismo , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Microscopia Crioeletrônica , Cristalografia por Raios X , Proteínas de Membrana Transportadoras/química , Proteínas de Membrana Transportadoras/genética , Simulação de Dinâmica Molecular , Periodontite/microbiologia , Porphyromonas gingivalis/genética , Porphyromonas gingivalis/crescimento & desenvolvimento , Conformação Proteica
10.
J Appl Oral Sci ; 28: e20190694, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32428060

RESUMO

Objective Obesity is a chronic disease that negatively affects an individual's general and oral health. The present study aimed to compare the clinical and microbiological effects of non-surgical periodontal therapy with the full mouth disinfection (FMD) protocol on obese and non-obese individuals at 9 months post-therapy. Methodology This clinical study was first submitted and approved by the Ethics Committee. Fifty-five obese patients and 39 non-obese patients with periodontitis were evaluated. The full-mouth periodontal clinical parameters, clinical attachment level (CAL), probing depth (PD), gingival index (GI), and plaque index (PI), were monitored at baseline, 3, 6, and 9 months after periodontal treatment with full mouth disinfection (FMD) protocol. The mean count of Tannerella forsythia , Porphyromonas gingivalis , Treponema Denticola , and Aggregatibacter actinomycetemcomitans was determined by quantitative polymerase chain reaction on subgingival biofilm samples. Demographic data were assessed by Chi-square test. For clinical and microbiological parameters, two-factor repeated-measures ANOVA was used. Results In both groups, periodontal therapy using the one-stage full-mouth disinfection protocol significantly improved CAL, PD, GI, and PI (p<0.05). Obese and non-obese patients equally responded to non-surgical periodontal therapy (p>0.05). Microbial count found no major differences (p>0.05) between obese and non-obese individuals who had undergone non-surgical periodontal therapy. Conclusions Obesity did not affect the clinical and microbiological outcomes of non-surgical periodontal therapy.


Assuntos
Obesidade/microbiologia , Periodontite/microbiologia , Periodontite/terapia , Adulto , Aggregatibacter actinomycetemcomitans/isolamento & purificação , Análise de Variância , Antropometria , Índice de Placa Dentária , Feminino , Humanos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Obesidade/fisiopatologia , Índice Periodontal , Porphyromonas gingivalis/isolamento & purificação , Estudos Prospectivos , Fatores de Risco , Estatísticas não Paramétricas , Tannerella forsythia/isolamento & purificação , Fatores de Tempo , Resultado do Tratamento , Treponema denticola/isolamento & purificação
11.
Med Sci (Paris) ; 36(5): 465-471, 2020 May.
Artigo em Francês | MEDLINE | ID: mdl-32452368

RESUMO

In the last decade, the association between the periodontitis and rheumatoid arthritis (RA) has been established, suggesting that oral microbiome plays a causal role by initiating this chronic autoimmune inflammatory disease of articulation. Both pathogenesis are similar in term of chronic inflammation, tissue breakdown and bone resorption. Molecular aspects have also revealed that citrullination, a post-translational modification catalyzed by peptidyl-arginine deiminases (PADs), is involved in both diseases. For RA, citrullinated proteins production leads to the synthesis the of anti-citrullinated protein antibodies triggering the loss of immune tolerance. In humans, five PADs have been identified. Recently, studies have found that only Porphyromonas species possess PAD. Thus, a major periodontal pathogen, Porphyromonas gingivalis, is able to generate citrullinated epitopes, and could consequently induce anti-citrullinated protein antibodies. In this review, citrullination process, periodontitis and RA are described to put them in relation with molecular, clinical and epidemiological studies establishing the association between periodontitis and RA.


Assuntos
Artrite Reumatoide/etiologia , Bactérias/enzimologia , Citrulinação/fisiologia , Microbiota/fisiologia , Boca/microbiologia , Desiminases de Arginina em Proteínas/fisiologia , Artrite Reumatoide/enzimologia , Artrite Reumatoide/metabolismo , Artrite Reumatoide/microbiologia , Bactérias/metabolismo , Interações entre Hospedeiro e Microrganismos/fisiologia , Humanos , Periodontite/complicações , Periodontite/enzimologia , Periodontite/microbiologia , Processamento de Proteína Pós-Traducional
12.
Arch Oral Biol ; 114: 104695, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32315811

RESUMO

OBJECTIVE: To analyse the citrulline level in the periodontium in association with the presence of or antibody levels against Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. DESIGN: Gingival crevicular fluid (GCF), subgingival biofilm and blood serum were sampled from 98 subjects (26 with RA, 72 without RA (NoRA)). GCF was analyzed for the level of citrulline, for interleukin (IL)-1ß, IL-17, IL-10 and monocyte-chemoattractant protein (MCP)-1. Microorganisms were identified in subgingival biofilms. Antibodies againstP. gingivalis, and Aggregatibacter actinomycetemcomitans were quantified in serum. RESULTS: GCF citrulline level was the lowest (by trend) in NoRA group without periodontitis. In NoRA, but not in RA an association between GCF citrulline level and P. gingivalis antibody levels was found and the GCF citrulline levels were higher in P. gingivalis positive samples. Any association of A. actinomycetemcomitans with GCF citrulline level did not exist. A model of univariate variance analysis (p = 0.001) showed a dependence of GCF citrulline level from the number of sites with PD (probing depth) ≥5 mm (p = 0.003) and the GCF MCP-1/CCL2 level (p = 0.019). Compared with NoRA in RA the number of teeth was lower, the number of sites with PD ≥ 5 mm was less, GCF levels of interleukin-17 and MCP-1/CCL2 were higher and those of IL-10 lower. Yeasts were only cultured in 15 RA patients (p < 0.001). CONCLUSION: Citrullination in periodontium might be associated with P. gingivalis supporting the potential role as a trigger in the development of RA. Pathogenesis of periodontal disease in RA patients seems to differ from that in NoRA and should be investigated further.


Assuntos
Artrite Reumatoide/complicações , Citrulinação , Citrulina/análise , Periodontite/microbiologia , Periodonto/química , Aggregatibacter actinomycetemcomitans , Infecções por Bacteroidaceae/patologia , Líquido do Sulco Gengival , Humanos , Periodonto/microbiologia , Porphyromonas gingivalis/patogenicidade
13.
Klin Lab Diagn ; 65(1): 55-60, 2020.
Artigo em Russo | MEDLINE | ID: mdl-32155008

RESUMO

Inflammatory periodontal diseases represent a serious dental and general medical problem due to the high prevalence among the adult population, the presence of clinical forms leading to the destruction of the dentition and tooth loss, insufficient treatment effectiveness and the frequency of relapse, including in connection with the formation of biofilms. A molecular genetic test system has been developed to evaluate the content of periodontopathogenic microorganisms Porphyromonas gingivalis, Treponema denticola, Streptococcus oralis, Streptococcus sanguis and Streptococcus sobrinus in the contents of periodontal pockets. The analytical characteristics of the test system were determined, and testing was carried out on clinical samples of patients with chronic generalized periodontitis of moderate severity. The constructed diagnostic kit allowed us to conduct a comparative analysis of the effectiveness of various types of treatment of inflammatory periodontal diseases based on quantitative data on the content of bacteria in the contents of periodontal pockets.


Assuntos
Bolsa Periodontal/microbiologia , Periodontite/diagnóstico , Periodontite/microbiologia , Adulto , Aggregatibacter actinomycetemcomitans , Bacteroides/isolamento & purificação , Diagnóstico Precoce , Testes Genéticos , Humanos , Porphyromonas gingivalis/isolamento & purificação , Streptococcus oralis/isolamento & purificação , Streptococcus sanguis/isolamento & purificação , Streptococcus sobrinus/isolamento & purificação , Treponema denticola/isolamento & purificação
14.
Braz Oral Res ; 34: e012, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32049112

RESUMO

Lipoproteins are important bacterial immunostimulating molecules capable of inducing receptor activator of nuclear factor-κB (RANKL) and osteoclast formation in vitro and in vivo . Although these molecules are present in periodontopathogenic bacteria, their role in periodontitis is not known. In this study, we used Pam2CSK4 (PAM2), a synthetic molecule that mimics bacterial lipoprotein, to investigate the effects of lipoproteins on periodontitis in mice. C57BL/6 male mice were randomly divided into three experimental groups: 1) Negative control group: animals received vehicle injection; 2) Positive control group: animals received injection of Escherichia coli lipopolysaccharide (LPS); 3) PAM2 group: animals received PAM2 injection. All the injections were performed bilaterally every other day into the palatal mucosa between first and second molars. After twenty-four days, the animals were euthanized to assess alveolar bone volume (micro-CT), cellular and extracellular composition in the gingiva (stereometric analysis), and osteoclast numbers (TRAP staining). Treatment with either PAM2 or LPS induced gingival inflammation, as demonstrated by increased infiltration of inflammatory cells and enhanced angiogenesis, associated with a smaller number of fibroblasts and decreased extracellular matrix. Importantly, treatment not only with LPS but also with PAM2 resulted in a larger number of TRAP+ multinucleated osteoclasts and significant loss of alveolar bone. Collectively, our data demonstrate that PAM2 can induce gingival inflammation and bone loss in mice, broadening the avenues of investigation into the role of lipoproteins in the pathogenesis of periodontal disease.


Assuntos
Lipopeptídeos/farmacologia , Periodontite/etiologia , Periodontite/patologia , Receptor 2 Toll-Like/antagonistas & inibidores , Perda do Osso Alveolar/etiologia , Perda do Osso Alveolar/patologia , Processo Alveolar/efeitos dos fármacos , Processo Alveolar/patologia , Animais , Modelos Animais de Doenças , Gengiva/efeitos dos fármacos , Gengiva/patologia , Gengivite/etiologia , Gengivite/patologia , Masculino , Camundongos Endogâmicos C57BL , Osteoclastos/efeitos dos fármacos , Osteoclastos/fisiologia , Periodontite/microbiologia , Distribuição Aleatória , Fosfatase Ácida Resistente a Tartarato , Fatores de Tempo , Microtomografia por Raio-X
15.
Infect Immun ; 88(5)2020 04 20.
Artigo em Inglês | MEDLINE | ID: mdl-32041789

RESUMO

Programmed death-ligand 1 (PD-L1/B7-H1) serves as a cosignaling molecule in cell-mediated immune responses and contributes to chronicity of inflammation and the escape of tumor cells from immunosurveillance. Here, we investigated the molecular mechanisms leading to PD-L1 upregulation in human oral carcinoma cells and in primary human gingival keratinocytes in response to infection with Porphyromonas gingivalis (P. gingivalis), a keystone pathogen for the development of periodontitis. The bacterial cell wall component peptidoglycan uses bacterial outer membrane vesicles to be taken up by cells. Internalized peptidoglycan triggers cytosolic receptors to induce PD-L1 expression in a myeloid differentiation primary response 88 (Myd88)-independent and receptor-interacting serine/threonine-protein kinase 2 (RIP2)-dependent fashion. Interference with the kinase activity of RIP2 or mitogen-activated protein (MAP) kinases interferes with inducible PD-L1 expression.


Assuntos
Antígeno B7-H1/metabolismo , Infecções por Bacteroidaceae/metabolismo , Carcinoma/metabolismo , Parede Celular/metabolismo , Neoplasias Bucais/metabolismo , Porphyromonas gingivalis/metabolismo , Proteína Serina-Treonina Quinase 2 de Interação com Receptor/metabolismo , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Infecções por Bacteroidaceae/microbiologia , Carcinoma/microbiologia , Linhagem Celular Tumoral , Gengiva/metabolismo , Gengiva/microbiologia , Humanos , Queratinócitos/metabolismo , Queratinócitos/microbiologia , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Neoplasias Bucais/microbiologia , Periodontite/metabolismo , Periodontite/microbiologia , Regulação para Cima/fisiologia
16.
BMC Oral Health ; 20(1): 27, 2020 01 30.
Artigo em Inglês | MEDLINE | ID: mdl-32000757

RESUMO

BACKGROUND: Both substance P and hypoxia-inducible factor 1 alpha (HIF-1α) are involved in inflammation and angiogenesis. However, the relationship between substance P and HIF-1α in rat periodontitis is still unknown. METHODS: Ligation-induced rat periodontitis was established to observe the distribution and expression of substance P and HIF-1α by immunohistochemistry. Rat gingival fibroblasts were cultured and stimulated with Porphyromonas gingivalis lipopolysaccharide (LPS). Recombinant substance P was applied to elaborate the relationship between substance P and HIF-1α in gingival fibroblasts in vitro. Primary mouse bone marrow-derived macrophages (BMMs) were isolated and cultured to observe the effect of substance P on receptor activator of NF-κB ligand (RANKL)-induced osteoclastogenesis by TRAP staining. Western blotting was used to investigate the expression of HIF-1α, osteoprotegerin (OPG) and RANKL. RESULTS: Rat experimental periodontitis was successfully established 6 weeks after ligation. Gingival inflammatory infiltration and alveolar bone loss were observed. Positive expression of substance P was found in the infiltrating cells. Higher HIF-1α levels were observed in periodontitis compared to that of normal tissues. Substance P upregulated the level of HIF-1α in gingival fibroblasts with or without 1 µg/ml LPS in vitro (*P < 0.05). Substance P upregulated the expression of HIF-1α in RANKL-stimulated BMMs in vitro. Substance P also increased the RANKL/OPG ratio in gingival fibroblasts (*P < 0.05). Both 10 nM and 50 nM substance P promoted RANKL-induced osteoclast differentiation (*P < 0.05). CONCLUSION: Substance P participates in periodontitis by upregulating HIF-1α and the RANKL/OPG ratio.


Assuntos
Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Osteoprotegerina/genética , Periodontite/metabolismo , Porphyromonas gingivalis/isolamento & purificação , Ligante RANK/genética , Substância P/genética , Animais , Regulação da Expressão Gênica , Gengiva/microbiologia , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Masculino , Camundongos , Osteoclastos , Osteoprotegerina/metabolismo , Periodontite/microbiologia , Porphyromonas gingivalis/genética , Ratos , Ratos Wistar , Substância P/metabolismo , Regulação para Cima/genética
17.
Biochim Biophys Acta Mol Basis Dis ; 1866(6): 165731, 2020 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-32088316

RESUMO

Outer membrane vesicles (OMVs) are nanosized particles derived from the outer membrane of gram-negative bacteria. Oral bacterium Porphyromonas gingivalis (Pg) is known to be a major pathogen of periodontitis that contributes to the progression of periodontal disease by releasing OMVs. The effect of Pg OMVs on systemic diseases is still unknown. To verify whether Pg OMVs affect the progress of diabetes mellitus, we analyzed the cargo proteins of vesicles and evaluated their effect on hepatic glucose metabolism. Here, we show that Pg OMVs were equipped with Pg-derived proteases gingipains and translocated to the liver in mice. In these mice, the hepatic glycogen synthesis in response to insulin was decreased, and thus high blood glucose levels were maintained. Pg OMVs also attenuated the insulin-induced Akt/glycogen synthase kinase-3 ß (GSK-3ß) signaling in a gingipain-dependent fashion in hepatic HepG2 cells. These results suggest that the delivery of gingipains mediated by Pg OMV elicits changes in glucose metabolisms in the liver and contributes to the progression of diabetes mellitus.


Assuntos
Membrana Externa Bacteriana/metabolismo , Cisteína Endopeptidases Gingipaínas/genética , Periodontite/genética , Porphyromonas gingivalis/genética , Animais , Membrana Externa Bacteriana/patologia , Modelos Animais de Doenças , Cisteína Endopeptidases Gingipaínas/metabolismo , Glicogênio Sintase Quinase 3 beta/genética , Humanos , Resistência à Insulina/genética , Fígado/metabolismo , Fígado/microbiologia , Camundongos , Periodontite/microbiologia , Periodontite/patologia , Porphyromonas gingivalis/metabolismo , Porphyromonas gingivalis/patogenicidade , Proteínas Proto-Oncogênicas c-akt/genética , Transdução de Sinais/genética
18.
Microb Pathog ; 140: 103962, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31904448

RESUMO

BACKGROUND AND OBJECTIVES: Recent evidence suggests that oral bacteria can affect extra-oral diseases by modulating aspects of the gut environment such as the microbiome, metabolome, and immune profiles. However, differences in the effects of different types of oral bacteria, particularly periodontopathic and health-associated bacteria, remain elusive. MATERIALS AND METHODS: Five-week-old germ-free mice were orally administered with either periodontopathic bacteria as oral pathobionts (Porphyromonas gingivalis, Filifactor alocis, and Fusobacterium nucleatum) or bacteria associated with periodontal health (Actinomyces naeslundii, Streptococcus mitis, and Veillonella rogosae) twice a week for five weeks. The presence of all bacterial species in the feces and the livers of the mice was analyzed via polymerase chain reaction (PCR), using specific primers for 16S rRNA genes. Alveolar bone resorption was evaluated histologically. The expression profiles of various genes in the liver and small intestine were analyzed using real-time PCR. Sera were analyzed to determine the levels of antibodies and endotoxin. The proportions of T helper 17 (Th17) and regulatory T (Treg) cells in mesenteric lymph nodes and Peyer's patches were analyzed using flow cytometry. RESULTS: Neither of the types of bacteria administered in this experiment induced alveolar bone resorption. All bacteria elicited some degree of systemic antibody response in the mice, although the response to S. mitis was not obvious. The response to P. gingivalis and V. rogosae was strongest. Generally, the health-associated bacteria but not the periodontitis-associated bacteria were detected in fecal samples. Interestingly, only Fusobacterium nucleatum DNA was detected in the liver, despite that live Fusobacterium nucleatum were not detected in the liver. The levels of interleukin-17 in the intestine and genes related to lipid accumulation in the liver were significantly higher in the mice that received periodontitis-associated bacteria. In addition, expression of the gene associated with endoplasmic reticulum stress was higher and that of the gene controlling circadian rhythm was lower in the periodontitis group. There was no difference in serum endotoxin, T-cell phenotypes in the lymphatic tissues, or genes related to the gut barrier. CONCLUSION: Oral administration of periodontitis-associated bacteria can induce pathological changes in the liver and intestine that are implicated in the process of periodontitis. These findings further support the importance of the oral-gut connection.


Assuntos
Boca/microbiologia , Periodontite/microbiologia , Simbiose , Actinomyces/fisiologia , Animais , Clostridiales/fisiologia , Feminino , Fusobacterium nucleatum/fisiologia , Vida Livre de Germes , Humanos , Interleucina-17/genética , Interleucina-17/imunologia , Intestinos/imunologia , Fígado/imunologia , Linfonodos/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Periodontite/genética , Periodontite/imunologia , Porphyromonas gingivalis/fisiologia , Streptococcus mitis/fisiologia , Linfócitos T Reguladores/imunologia , Células Th17/imunologia , Veillonella/fisiologia
19.
Acta Odontol Scand ; 78(5): 327-331, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31980002

RESUMO

Objective: Type 2 diabetes mellitus (T2DM) is a well-defined risk factor of periodontitis and it can affect expression of human beta-defensins (hBDs) and cathelicidin (LL-37) as well. The aim of the present study was to evaluate the impact of periodontitis and T2DM on salivary concentrations of these antimicrobial peptides.Material and methods: Unstimulated saliva samples, together with full-mouth periodontal recordings were collected from 92 individuals with periodontitis (63 with T2DM and 21 smokers) and 86 periodontally healthy controls (58 with T2DM and 21 smokers). Salivary hBD-1, -2, -3, LL-37, and advanced glycalization end products (AGE) concentrations were measured by enzyme-linked immunosorbent assay.Results: Among the periodontitis patients, T2DM group demonstrated lower levels of hBD-1 (p = .006), hBD-2 (p < .001) and hBD-3 (p < .001), and higher levels of LL-37 (p < .001) compared to systemically healthy controls. When only periodontally healthy controls were included into the analysis, higher hBD-1 (p = .002) and LL-37 (p < .001) levels were found in T2DM patients in comparison to systemically healthy controls. Salivary LL-37 levels were associated with HbA1c and periodontitis, while hBD-2, hBD-3 and levels associated only with HbA1c.Conclusion: In the limits of this study, hyperglycaemia can be proposed as a regulator of salivary hBD and cathelicidin levels. Periodontitis, on the other hand, affects only salivary LL-37 levels.


Assuntos
Peptídeos Catiônicos Antimicrobianos/metabolismo , Diabetes Mellitus Tipo 2/sangue , Periodontite/sangue , Saliva/química , beta-Defensinas/sangue , Adulto , Peptídeos Catiônicos Antimicrobianos/sangue , Estudos de Casos e Controles , Diabetes Mellitus Tipo 2/complicações , Hemoglobina A Glicada , Humanos , Pessoa de Meia-Idade , Periodontite/microbiologia , Periodontite/terapia , Saliva/metabolismo , beta-Defensinas/metabolismo
20.
J Med Microbiol ; 69(2): 298-308, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31976854

RESUMO

Introduction. Periodontitis is among the most widespread oral bacterial diseases affecting 15-20% of the world population.Aim. This study aimed to develop dental floss impregnated with povidone-iodine (PVP-I) as an antimicrobial delivery system against periodontopathogenic bacteria in a planktonic form and within biofilms.Methods. Identical lengths of dental floss impregnated with PVP-I formulations were placed on agar along with previously grown periodontal pathogens. The bioactivity of the dental floss was investigated by response-surface methodology. In order to explore the antibacterial activity of the selected formulation and the potential application in the prevention and treatment of plaque-caused diseases such as periodontitis and caries, the antibacterial and anti-biofilm activity of the selected PVP-I formulation against pathogenic bacteria were investigated.Results. The results indicated that the coating formulation containing Eudragit L-100 2.90 %, PVP-I 24.58 % and PEG 400 3.73 % had antimicrobial activity for all pathogens. The mechanism of this formulation involved disruption of bacterial cell membranes. Moreover, this formulation inhibited the formation of oral pathogenic biofilms.Conclusion. It was concluded that Eudragit L-100 and PVP-I-coated dental floss represented a potential therapeutic agent to prevent periodontal diseases and dental caries and exhibited non-toxicity to periodontal ligament cells.


Assuntos
Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Periodontite/prevenção & controle , Ácidos Polimetacrílicos/química , Povidona-Iodo/farmacologia , Antibacterianos/química , Bactérias/crescimento & desenvolvimento , Dispositivos para o Cuidado Bucal Domiciliar , Sistemas de Liberação de Medicamentos , Humanos , Periodontite/microbiologia , Povidona-Iodo/química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA