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1.
J Biol Regul Homeost Agents ; 35(1 Suppl. 1): 1-9, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33463138

RESUMO

Periodontal diseases (PDs) affect about half of the adult population all over the world. PDs are caused by bacterial infection which induces an inflammatory response with progressive destruction of the periodontal tissues and finally the loss of teeth. Tobacco smoking (TS), alcohol consumption, and systemic diseases (SDs), are considered additional risk factors. This short review examines the potential causal association between PDs, TS and SDs. There is strong evidence that PDs are associated with an increased risk of SDs. In addition, many patients with SDs are also affected by PD, which can be mild or severe, and tobacco smokers manifest a greater risk of developing PDs. This paper includes many randomized controlled trials and reviews to test the effects of different periodontal therapies for patients with SDs.


Assuntos
Doenças Periodontais , Humanos , Doenças Periodontais/epidemiologia , Doenças Periodontais/etiologia , Periodonto , Fatores de Risco , Fumantes
2.
Orthod Fr ; 91(1-2): 41-46, 2020 06 01.
Artigo em Francês | MEDLINE | ID: mdl-33146131

RESUMO

For several decades, orthodontists and periodontists have repeated that orthodontics have no harmful consequences on the periodontium when it is healthy or treated. However, a systematic review of the literature undermines this common refrain and concludes that there is a lack of reliable evidences of the positive effects of orthodontics on periodontal health with, in the best periodontal conditions, slight adverse effects. It is therefore the responsibility of orthodontists to keep the periodontal cost of orthodontic treatment as low as possible. How to make sure that this « at best ¼ does not turn into an « at worst ¼ ? In order to minimize the deleterious consequences of orthodontic treatment on the periodontium, the orthodontist must be able to specify which patients she/he can immediately consider providing orthodontic treatment and those on whom periodontal treatment is mandatory before all. The orthodontist must therefore transform, for a few minutes, into a periodontist in order to recognize the eight signs of loss of attachment and the six risk factors for periodontitis, exposed in this article. Both needs for periodontal and orthodontic treatment have to be measured and would not be efficient without the patient's and the practitioners' motivation.


Assuntos
Ortodontia , Doenças Periodontais , Odontólogos , Feminino , Humanos , Masculino , Doenças Periodontais/terapia , Periodontia , Periodonto
3.
Shanghai Kou Qiang Yi Xue ; 29(4): 350-354, 2020 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-33089280

RESUMO

PURPOSE: To investigate the expression and significance of chemokines CCL21, E-selectins and heat shock protein 90 (Hsp90) in periodontal tissues of rats with experimental periodontitis. METHODS: Forty 10-week-old male Wistar rats were significantly randomly divided into 4 groups with 10 rats in each group. Periodontitis models were established in groups A, B and C, and the rest were 10 blank control groups. Rats in group A, B and C were sacrificed at 4, 8 and 12 weeks after basic periodontal treatment, and the periodontal tissues of the first and second molars were taken for CCL21, E-selectins and Hsp90 protein expression detection. SPSS 25.0 software package was used to analyze the data. RESULTS: The levels of periodontal attachment in group A, B and C were higher than those in the control group(P<0.05). The levels of periodontal attachment, CCL21, E-selectins, Hsp90 mRNA and protein expression in periodontal tissues increased first and then decreased(P<0.05). The levels of periodontal attachment, CCL21, E-selectins, Hsp90 mRNA and protein expression in group B and C were significantly higher than those in group A(P<0.05). The levels of periodontal attachment, CCL21, E-selectins, Hsp90 and relative protein expression in periodontal tissues of group C were significantly lower than those of group B(P<0.05). CONCLUSIONS: The expression of CCL21, E-selectins and Hsp90 is up-regulated in periodontitis tissues. With local periodontal treatment, the expression level of CCL21, E-selectins and Hsp90 gradually decreases.


Assuntos
Selectina E , Periodontite , Animais , Quimiocina CCL21/metabolismo , Proteínas de Choque Térmico HSP90/metabolismo , Masculino , Dente Molar , Periodontite/genética , Periodonto , Ratos , Ratos Wistar
4.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 55(10): 710-715, 2020 Oct 09.
Artigo em Chinês | MEDLINE | ID: mdl-33045780

RESUMO

Accurate and measurable tooth preparation under the principle of protecting dental tissue, vital pulp and periodontal tissue health is a challenging procedure in esthetic dental restoration. The traditional protocol of tooth preparation often leads to complications after restoration because of the lack of precise preparation control. The pre-operative analysis and design, intraoperative guidance and sequence guidance technology of post-operative prosthesis production, with the target restoration space (TRS) as the core and digital technology as the support technology, embody the numerical requirements of the prosthesis space based on the actual measurement, and will lay the core foundation of the future digital prosthodontics. This paper introduces the new understanding of tooth preparation guidance, the classification and application of digital guides, the practice of micro tooth preparation guided by digital template, and summarizes the development direction of prosthodontics from experience guidance to digital guidance.


Assuntos
Estética Dentária , Preparo do Dente , Polpa Dentária , Periodonto , Prostodontia
5.
J Contemp Dent Pract ; 21(7): 798-802, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-33020366

RESUMO

AIM: To illustrate, with two clinical cases of endoperiodontal lesions, the clinical application of the new classification of periodontal and peri-implant diseases and conditions. BACKGROUND: The endodont and the periodont are two entities that communicate with each other through physiological communication channels (apical foramen, lateral and secondary canals, and dentinal tubules) resulting in close anatomical and functional interaction. An endoperiodontal lesion is defined by pathological communication between the endodontic and periodontal tissues in a given tooth, according to the definition given by the new classification of periodontal and peri-implant diseases and conditions from the work of the Chicago Consensus Conference in 2017. This new classification differentiates the lesions with and without root damage. Diagnosis and therapeutic strategy will be analyzed through two clinical cases. REVIEW RESULTS: The clinical cases we presented show that the treatment of these lesions must involve endodontic and periodontal management due to the intimate relationship between the tooth and periodontium. CONCLUSION: The classification of periodontal and peri-implant diseases and conditions provides a clinical focus on endoperiodontal lesions, based on signs and symptoms that have a direct effect on the prognosis and the treatment of the tooth. The pathological communication between the endodont and the periodontium complicates the management of the involved tooth. CLINICAL SIGNIFICANCE: Chicago's new classification of periodontal and peri-implant diseases and conditions offers an up-to-date vision of periodontal lesions management and highlights the intimate links between endodontic and periodontal tissues.


Assuntos
Peri-Implantite , Doenças Periodontais , Chicago , Humanos , Ligamento Periodontal , Periodonto
6.
Shanghai Kou Qiang Yi Xue ; 29(3): 298-303, 2020 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-33043348

RESUMO

PURPOSE: To explore the percentage of residual support height (Ph) and the percentage of residual root surface area (Ps) in evaluating periodontal support ability by simulating different stages of periodontitis based on the curved surface modeling. METHODS: Fifteen cone-beam CT (CBCT) images including 420 teeth in total were collected. The data were reconstructed into 3-dimensional teeth models by Mimics software.The 3D surface model of the tooth was then optimized by Geomagic software and then imported into Solidworks software to simulate different periodontal support height. Ph and Ps were measured and calculated to evaluate the consistency of Ph and Ps results in all tooth types. The data were analyzed with SPSS 22.0 software package. RESULTS: RSA in incisors, canines and premolars: coronal 1/3>middle 1/3>apical 1/3. RSA in molars: middle 1/3>coronal 1/3>apical 1/3. Maxillary first molar had the largest RSA, accounting for 11.60% of the dentition, which was about 3.18 times than mandibular central incisor. The difference between Ph and Ps in all types of teeth was statistically significant (P<0.01). The 95% confidence interval(CI) of the difference between Ph and Ps in the maxillary incisor, mandibular incisor, mandibular canine was between the clinical consistency limit (-15%, 15%). In the remaining tooth types, 95%CI of the difference between Ph and Ps was beyond the clinical consistency limit (-15%, 15%). CONCLUSIONS: For single-root tooth, except maxillary canine, the remaining periodontal support height could replace periodontal support area. For multi-rooted tooth, judging the ability of periodontal support ability only by alveolar bone absorption ratio in 2D index has significant limitations. Full consideration is needed to focus on root morphological discrepancy when determining the extent of periodontal disease.


Assuntos
Periodonto , Raiz Dentária , Dente Pré-Molar , Tomografia Computadorizada de Feixe Cônico , Incisivo , Periodonto/diagnóstico por imagem
7.
Shanghai Kou Qiang Yi Xue ; 29(3): 293-297, 2020 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-33043347

RESUMO

PURPOSE: To explore the efficacy and safety of ornidazole combined with periodontal tissue regeneration in the treatment of periodontitis. METHODS: From March 2018 to March 2019, 100 patients with periodontitis who received treatment in the Stomatological Hospital Affiliated to the School of Medicine of Nanjing University were selected and randomly divided into the regeneration group and combined treatment group with 50 patients in each group. Patients in the regeneration group received periodontal tissue regeneration treatment, while patients in the combined treatment group received ornidazole combined periodontal tissue regeneration treatment. Related periodontal indexes including periodontal probing depth(PPD), periodontal attachment level(PAL), tooth mobility degree(MD) were measured, serum malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (gsh-px) and interleukin 10 (IL-10) and interleukin 4 (IL-4), c-reactive protein(CRP) level and immune globulin level were detected before and after treatment, the therapeutic effects and complications were recorded and compared. SPSS 21.0 software package was used for statistical analysis of the data. RESULTS: After treatment, PPD, PAL and MD levels in the combined treatment groups were significantly lower than those in the regenerative group (P<0.05). Serum MDA level in the combined treatment group was significantly lower than that in the regenerative group, SOD and gsh-px levels were significantly higher than that in the regenerative group(P<0.05). The serum levels of IgA, IgM, IgG, IgE, IL-10, IL-4 and CRP in the combined treatment group were significantly lower than those in the regenerative treatment group (P<0.05). The total effective rate of the combined treatment group was significantly higher than that of the regenerative treatment group, and the incidence of complications was significantly lower than that of the regenerative group(P<0.05). CONCLUSIONS: Ornidazole combined with periodontal tissue regeneration can improve the level of periodontal index, alleviate oxidative stress injury, improve immune function, inhibit inflammation, and has a significant therapeutic effect with high safety.


Assuntos
Metronidazol , Periodontite , Terapia Combinada , Humanos , Metronidazol/efeitos adversos , Índice Periodontal , Periodontite/tratamento farmacológico , Periodonto
8.
Wiad Lek ; 73(8): 1761-1764, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33055348

RESUMO

OBJECTIVE: The aim: To investigate current trends in the study of oral biofilm and its control. PATIENTS AND METHODS: Materials and methods: A research of 32 literature sources has been made and it has been taken into account that some terminological differences in determining objects of study. CONCLUSION: Conclusions: Detailed analysis of modern domestic and foreign literature argues the necessity of further in-depth study of oral biofilms. Understanding the ethiological factors and mechanisms of the pathogenesis periodontal tissues inflammatory diseases gives the opportunity to treat targetly by destroying complicated sections of the vital activities and oral biofilm microorganisms relationships.


Assuntos
Doenças Periodontais , Biofilmes , Humanos , Periodonto
9.
J Int Acad Periodontol ; 22(3): 174-181, 2020 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-32980830

RESUMO

AIMS: The purpose of this study was to evaluate the impact of fullfixed orthodontic appliances on the periodontium in adult patients. METHODS: Seventeen periodontally and systemically healthy subjects were selected from the Periodontal Clinic of Guarulhos University, 7 males and 10 females (mean age: 38.3 ± 6.3 years). The patients undergoing orthodontic treatment were submitted a clinical examination, a cone beam computed tomography at baseline and after 12 months of treatment. Subgingival biofilm samples were analyzed by Checkerboard DNA-DNA hybridization. Statistical analysis was performed by a Wilcoxon test. RESULTS: The percentage of sites with visible plaque increased (p =0.003), but no significant reduction in marginal bone was observed. The mean periodontal pocket depth was reduced (p=0.001) and the clinical attachment level significantly improved (p =0.001). There was a significant reduction in the mean proportions of the Actinomyces sp and an increase in the orange complex species. The proportions of the red complex species remained unchanged. CONCLUSIONS: In spite of increase in plaque accumulation no significant clinical or tomographic iatrogenic changes in periodontally healthy adults undergoing orthodontic full-fixed appliance treatment could be detected. The microbiological changes did not affect the periodontal parameters in monitored adult patients that received short period of orthodontic treatment.


Assuntos
Placa Dentária , Microbiota , Adulto , Feminino , Humanos , Masculino , Aparelhos Ortodônticos Fixos/efeitos adversos , Bolsa Periodontal , Periodonto
10.
Int J Periodontics Restorative Dent ; 40(5): e197-e204, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32925995

RESUMO

The relationship between attachment loss and occlusal trauma has been debated for many years. When a patient presents with advanced periodontal disease, a decision has to be made on whether the teeth can be saved or extracted. In this treatment example, the decision process in therapeutic planning for a patient with stage IV periodontal disease is discussed. The main dilemma is whether the patient should receive a prosthodontic reconstruction supported by osseointegrated implants or by periodontally compromised natural teeth. It is assumed that implants do better than teeth over the long term based on firm documentation in the literature, but this article describes why a periodontal prosthesis is still a viable treatment option.


Assuntos
Implantes Dentários , Doenças Periodontais , Dente , Implantação Dentária Endo-Óssea , Humanos , Ligamento Periodontal , Periodonto
11.
PLoS One ; 15(9): e0238659, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32941479

RESUMO

Adjunctive use of laser devices as high reactive-level laser/light therapy (HLLT) or photobiomodulation therapy (PBMT) for periodontal therapy is known to be more effective on suppressing pain than conventional therapy, however, there are no systematic reviews addressed its effectiveness. This systematic review and meta-analysis aim to investigate the following clinical question (CQ): does adjunctive use of lasers with conventional therapy suppress the pain associated with periodontal treatment? A systematic and extensive literature search was performed to summarize the currently available knowledge to answer the CQ using the PubMed, Cochrane Library, and Web of Science databases for randomized controlled trials (RCTs) conducted before June 2020. Bias risk was assessed using the Cochrane tool for the risk of bias evaluation. A meta-analysis was performed on quantitative evaluation of pain control based on patient-reported outcomes. After an independent screening of 165 initial records, ten RCTs were included. Six of them focused on surgical procedures and the others on non-surgical periodontal pocket therapy. The protocols of HLLT, PBMT, and combination with HLLT and PBMT were employed in five, four and one RCTs, respectively. Following the assessment of bias risk, it is revealed that all RCTs had methodological weaknesses regarding the blinding of key personnel, although other bias risk factors were not evident. Meta-analysis showed that HLLT using erbium lasers significantly reduced the patient-reported pain immediately after treatment (two RCTs, p < 0.0001), while PBMT using diode lasers significantly reduced pain 2-7 days after treatment (two RCTs, p < 0.0001 to p = 0.03). The presented systematic review and meta-analysis suggest that the alternative use of HLLT using erbium lasers to conventional instrumentation can significantly suppress postoperative pain and that intraoperative or postoperative PBMT using diode lasers combined with periodontal surgery can significantly reduce postoperative pain. However, the evidence is still insufficient and more well-designed RCTs are required.


Assuntos
Terapia a Laser , Manejo da Dor , Medidas de Resultados Relatados pelo Paciente , Periodonto/cirurgia , Humanos , Julgamento , Terapia com Luz de Baixa Intensidade , Viés de Publicação , Risco , Escala Visual Analógica
12.
J Med Life ; 13(2): 225-228, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32742518

RESUMO

The high prevalence of periodontal diseases in workers with professional contact with unfavorable factors of the production environment is an unresolved problem of dentistry. This study aimed to investigate the harmful effects of formaldehyde on periodontal tissues in woodworkers who have long-term contact with formaldehyde in their professional activities. Sixty-nine men with occupational exposure to formaldehyde were examined to study the effect of formaldehyde on the human periodontal tissues, looking particularly at signs of the periodontal tissues' inflammatory process using a series of periodontal indices. The study results showed that the condition of periodontal tissues was statistically significantly worse in woodworkers who have long-term contact with formaldehyde in their professional activities. However, the hygiene status was not significantly different in the main group and the comparison group. Thus, we concluded that working under conditions of constant exposure to formaldehyde has a negative effect on the condition of periodontal tissues.


Assuntos
Formaldeído/efeitos adversos , Indústrias , Exposição Ocupacional/análise , Periodonto/efeitos dos fármacos , Adulto , Humanos , Masculino , Pessoa de Meia-Idade , Saúde Bucal , Prevalência , Adulto Jovem
13.
Periodontol 2000 ; 84(1): 45-68, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32844417

RESUMO

Periodontitis is a highly prevalent disease. As it progresses, it causes serious morbidity in the form of periodontal abscesses and tooth loss and, in the latter stages, pain. It is also now known that periodontitis is strongly associated with several nonoral diseases. Thus, patients with periodontitis are at greater risk for the development and/or exacerbation of diabetes, chronic obstructive pulmonary disease, and cardiovascular diseases, among other conditions. Although it is without question that specific groups of oral bacteria which populate dental plaque play a causative role in the development of periodontitis, it is now thought that once this disease has been triggered, other factors play an equal, and possibly more important, role in its progression, particularly in severe cases or in cases that prove difficult to treat. In this regard, we allude to the host response, specifically the notion that the host, once infected with oral periodontal pathogenic bacteria, will mount a defense response mediated largely through the innate immune system. The most abundant cell type of the innate immune system - polymorphonuclear neutrophils - can, when protecting the host from microbial invasion, mount a response that includes upregulation of proinflammatory cytokines, matrix metalloproteinases, and reactive oxygen species, all of which then contribute to the tissue damage and loss of teeth commonly associated with periodontitis. Of the mechanisms referred to here, we suggest that upregulation of reactive oxygen species might play one of the most important roles in the establishment and progression of periodontitis (as well as in other diseases of inflammation) through the development of oxidative stress. In this overview, we discuss both innate and epigenetic factors (eg, diabetes, smoking) that lead to the development of oxidative stress. This oxidative stress then provides an environment conducive to the destructive processes observed in periodontitis. Therefore, we shall describe some of the fundamental characteristics of oxidative stress and its effects on the periodontium, discuss the diseases and other factors that cause oxidative stress, and, finally, review potentially novel therapeutic approaches for the management (and possibly even the reversal) of periodontitis, which rely on the use of therapies, such as resveratrol and other antioxidants, that provide increased antioxidant activity in the host.


Assuntos
Periodontite , Citocinas , Humanos , Inflamação , Estresse Oxidativo , Periodonto
14.
Georgian Med News ; (303): 125-134, 2020 Jun.
Artigo em Russo | MEDLINE | ID: mdl-32841194

RESUMO

A side effect of treating children with fixed orthodontic equipment (FOE) is the deterioration of the oral microbiota and, as a typical consequence, a significant increase in the risk of chronic catarrhal gingivitis (CCG). The problem is to calculate the specified risk for each specific child, and its modern solution using models subjected to the machine learning procedure requires the identification of microbial and other markers of the process of formation of oral microbiota in the presence of FOE in healthy children and children with CCG. The identification of such markers was the main goal of this study. The experiment consisted in monitoring this process by four dental and nineteen microbial parameters at the third, sixth and twelfth month of orthodontic treatment of 30 non-sick and 32 patients with CCG having healthy periodontal tissues before the FOE. Markers were detected by correlation, principal component (PCA), factor and cluster analyzes of the obtained experimental data. As a result, a marker rating of the studied microbial and dental parameters was built, and most importantly, it was shown that the microorganisms Veillonella spp., Neisseria spp., Lactobacillus spp., Enterobacteriaceae spp. and some others have pronounced properties of markers of the process of formation of oral microbiota in the presence of FOE in healthy children and children with CCG.


Assuntos
Gengivite , Microbiota , Criança , Doença Crônica , Humanos , Periodonto
15.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 55(8): 591-594, 2020 Aug 09.
Artigo em Chinês | MEDLINE | ID: mdl-32842352

RESUMO

Tooth loss caused by trauma, periodontitis or inherited disorders severely affects human physical and mental health. As an essential part of the tooth, tooth root is connected to periodontal tissues to maintain the tooth in the alveolar socket. To figure out the molecular mechanisms regulating tooth root development will contribute to the discovery of new approaches in tooth root regeneration. The development of tooth root is a complicated process involving communication between the epithelial and mesenchymal tissues and the regulation of multiple signaling pathways. The present article reviewed the research progress of the signaling pathways in tooth root development.


Assuntos
Raiz Dentária , Dente , Humanos , Odontogênese , Ligamento Periodontal , Periodonto
16.
Ned Tijdschr Tandheelkd ; 127(6): 358-364, 2020 Jun.
Artigo em Holandês | MEDLINE | ID: mdl-32716399

RESUMO

Gingivitis and periodontitis can worsen with reduced immune fitness. Various causes can reduce immune fitness in a host, as a result of which the balance between the host and the microbiome is disturbed. Among others, lifestyle factors, such as stress and smoking, can have a negative influence on immune fitness. An association has been demonstrated between stress and periodontitis and also acute necrotising ulcerative gingivitis or periodontitis. There are indications that neurons are able to secrete pro-inflammatory cytokines and chemokines that worsen chronic inflammatory reactions in the periodontium and compromise immune fitness. In vitro studies show high cortisol levels may contribute to the increased growth of P. gingivalis. Stress as a risk factor for periodontitis and the role of stress as a negative influence on the results of periodontal treatment are difficult to estimate clinically. Nevertheless, attention to and awareness of stress as an aspect of the comprehensive set of risk factors for periodontitis can diminish its negative impact on immune fitness.


Assuntos
Gengivite , Periodontite , Humanos , Inflamação , Periodonto
17.
J Appl Oral Sci ; 28: e20190766, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32638830

RESUMO

Objective To compare two corticotomy surgical protocols in rats to verify whether they alter conventional orthodontic movement. Methodology Sixty Wistar rats were divided into three groups - orthodontic movement (CG), orthodontic movement and corticotomy (G1) and orthodontic movement with corticotomy and decortication (G2) - and euthanized after 7 and 14 days. Tooth movement (mm), bone volume fraction and bone volume ratio to total volume (BV/TV), and bone mineral density (BMD) were evaluated by micro-CT. The total amount of bone was measured in square millimeters and expressed as the percentage of bone area in the histomorphometry. The number of positive TRAP cells and RANK/RANKL/OPG interaction were also investigated. Results Day 14 showed a statistically significant difference in orthodontic tooth movement in CG compared with G1 (7.52 mm; p=0.009) and G2 (7.36 mm; p=0.016). A micro-CT analysis revealed a difference between CG, G1 and G2 regarding BV/TV, with G1 and G2 presenting a lower BV/TV ratio at 14 days (0.77 and 0.73 respectively); we found no statistically significant differences regarding BMD. There was a difference in the total amount of bone in the CG group between 7 and 14 days. At 14 days, CG presented a significantly higher bone percentage than G1 and G2. Regarding TRAP, G2 had more positive cells at 7 and 14 days compared with CG and G1. Conclusion Corticotomy accelerates orthodontic movement. Decortication does not improve corticotomy efficiency.


Assuntos
Processo Alveolar , Técnicas de Movimentação Dentária , Animais , Periodonto , Ratos , Ratos Wistar , Microtomografia por Raio-X
18.
Int J Nanomedicine ; 15: 4021-4047, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32606662

RESUMO

Purpose: Periodontitis is a chronic inflammatory disease associated with microbial accumulation. The purpose of this study was to reuse the agricultural waste to produce cellulose nanofibers (CNF) and further modification of the CNF with κ-carrageenan oligosaccharides (CO) for drug delivery. In addition, this study is focused on the antimicrobial activity of surfactin-loaded CO-CNF towards periodontal pathogens. Materials and Methods: A chemo-mechanical method was used to extract the CNF and the modification was done by using CO. The studies were further proceeded by adding different quantities of surfactin [50 mg (50 SNPs), 100 mg (100 SNPs), 200 mg (200 SNPs)] into the carrier (CO-CNF). The obtained materials were characterized, and the antimicrobial activity of surfactin-loaded CO-CNF was evaluated. Results: The obtained average size of CNF and CO-CNF after ultrasonication was 263 nm and 330 nm, respectively. Microscopic studies suggested that the CNF has a short diameter with long length and CO became cross-linked to form as beads within the CNF network. The addition of CO improved the degradation temperature, crystallinity, and swelling property of CNF. The material has a controlled drug release, and the entrapment efficiency and loading capacity of the drug were 53.15 ± 2.36% and 36.72 ± 1.24%, respectively. It has antioxidant activity and inhibited the growth of periodontal pathogens such as Streptococcus mutans and Porphyromonas gingivalis by preventing the biofilm formation, reducing the metabolic activity, and promoting the oxidative stress. Conclusion: The study showed the successful extraction of CNF and modification with CO improved the physical parameters of the CNF. In addition, surfactin-loaded CO-CNF has potential antimicrobial activity against periodontal pathogens. The obtained biomaterial is economically valuable and has great potential for biomedical applications.


Assuntos
Carragenina/química , Celulose/química , Lipopeptídeos/química , Nanofibras/química , Peptídeos Cíclicos/química , Periodonto/microbiologia , Animais , Bactérias/metabolismo , Compostos de Bifenilo/química , Sobrevivência Celular , Difusão Dinâmica da Luz , Depuradores de Radicais Livres/química , Malondialdeído/metabolismo , Camundongos , Testes de Sensibilidade Microbiana , Nanofibras/ultraestrutura , Oligossacarídeos/química , Picratos/química , Células RAW 264.7 , Soja/química , Espectroscopia de Infravermelho com Transformada de Fourier
19.
Stomatologiia (Mosk) ; 99(3): 32-36, 2020.
Artigo em Russo | MEDLINE | ID: mdl-32608946

RESUMO

The aim of the study was to evaluate the diagnostic value of determining the level of hypoxia-inducible factor-1α (HIF-1α) in the gingival fluid in patients with chronic generalized periodontitis (CGP) and exacerbation of inflammatory periodontal diseases in patients with cancer of the oral mucosa (OM). A clinical study was carried out with the participation of 60 patients with CGP (group 1) and 22 patients with cancer of OM (group 2) of the T1-2N0M0 stage, as well as 15 healthy individuals with no revealed periodontal pathology. CGP patients, depending on the depth of periodontal pockets (PC), were divided into two subgroups - with a mild degree (PC depth up to 3.5 mm) and an average degree (PC depth from 4 to 6 mm). Group 2 patients were also divided into two subgroups - with exacerbation of CGP (n=12) and patients with no complications (n=10). The content of HIF-1α in the gingival fluid was determined by enzyme immunoassay. The results of the study made it possible to establish that in patients of group 1, the concentration of HIF-1α in inflammatory exudate increased by 2.4 times (p<0.001) compared with the control group. The concentration of HIF-1α in the contents of periodontal pockets in patients with CGP depended on the severity of the disease: with mild CGP, it did not differ from the control group, and with moderate CGP it was 3.15 times higher (p<0.001). In patients with cancer of OM, the concentration of HIF-1α in the gingival fluid did not depend on the presence of inflammatory periodontal lesions and was determined by a multiple increase in the biomarker level (7.5 times) due to the development of a malignant process in the oral cavity. Therefore, monitoring the concentration of HIF-1α in the gingival fluid is informative in patients with CGP in assessing the severity of periodontal conditions, as well as in differential diagnosis with malignant formations of the oral cavity.


Assuntos
Periodontite Crônica , Periodonto , Líquido do Sulco Gengival , Humanos , Hipóxia , Ligamento Periodontal , Bolsa Periodontal
20.
Georgian Med News ; (302): 28-33, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32672685

RESUMO

Implant therapy, a modern treatment option for completely and partially edentulous patients. However, numerous clinical studies indicate a sufficiently large number (9-20%) of the failure rate of implants. The achievement of osseointegration depends on many factors, such as a suitable host, alteration in bone metabolism, hormone balance, smoking habits, and local conditions (quantity and quality of bone, biocompatible materials, traumatic surgical technique, occlusal conditions, quality of dental implants, and corrosion of the metal of implants, thoroughness of surgery and healing time). As can be seen from the review data, achievements in the field of dental implantation are based on a deep understanding of the fundamental cellular processes of regeneration and restoration of periodontal tissues, maintaining optimal local conditions that contribute to accelerating the regeneration process. A thorough knowledge of the cellular mechanisms of wound healing and the clinical consequences of modern periodontal treatments should provide further improvement or the development of new treatment strategies for the restoration and regeneration of soft tissues and dental implants. Various immuno-inflammatory mediators and bone-related molecules can be considered potential biomarkers that will be used for monitoring peri-implant health and disease. Their timely detection and systematic maintenance therapy may have a crucial impact on decreasing the occurrence of peri-implant lesions. Importantly, individual genetic data might contribute to therapeutic planning and prognosis, make its coordination depending on observed genotype. Supplementary evidence concerning the molecular pathophysiology of peri-implantitis may, in the future, benefit the treatment of dental implants, development of preventive approaches to avoid peri-implant tissue breakdown.


Assuntos
Implantes Dentários , Boca Edêntula , Peri-Implantite , Humanos , Osseointegração , Peri-Implantite/terapia , Periodonto , Cicatrização
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