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1.
J Contemp Dent Pract ; 21(2): 119-123, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32381813

RESUMO

AIM: The aim of this study is to compare the efficacy of dipotassium oxalate and potassium nitrate to occlude dentinal tubules. MATERIALS AND METHODS: This study utilized Parkinson model of longitudinal dentin tubule occluding properties of dentifrices under a 4-day acid challenge. Dentin disks of approximately 1.5 mm thick were sectioned from the crowns of the freshly extracted molars. The disks were randomized into three sets of 15 and treated with dipotassium oxalate, potassium nitrate, or used as a control. The disks were then subjected to a 4-day acid challenge and evaluated by scanning electron microscopy (SEM). RESULTS: On days 1, 2, and 3, dipotassium oxalate showed significant occlusion of dentinal tubules. On day 4, no significant difference was observed between dipotassium oxalate and potassium nitrate. Both test groups showed better occlusion properties in comparison to the control. CONCLUSION: Through the use of a 4-day acid challenge, this study demonstrates that both agents can indeed occlude dentinal tubules. Initially, dipotassium oxalate does occlude dentinal tubules faster than potassium nitrate. However, at the conclusion of the acid challenge, minimal differences were observed in occlusion rate among the two agents. Further studies should be conducted to determine the efficacy of these two agents. CLINICAL SIGNIFICANCE: Both dipotassium oxalate and potassium nitrate can help treat patients with dentinal hypersensitivity.


Assuntos
Permeabilidade da Dentina , Ácido Oxálico , Dentina , Humanos , Nitratos , Compostos de Potássio
2.
J Indian Soc Pedod Prev Dent ; 38(1): 64-70, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32174631

RESUMO

Introduction: The chemomechanical preparation of root canal dentin with hand or rotary instruments creates debris and a smear layer. Root canal preparation (RCP) along with irrigants is not effective in cleaning apical portions, and hence, different laser activation systems were used for better cleaning in the apical third. Aim: The aim of this study is to compare the efficacy of erbium: yttrium-aluminum-garnet (Er:YAG) and diode laser irradiation in smear layer removal and dentin permeability after biomechanical preparation using scanning electron microscopic investigation. Material and Methods: Thirty sound single-rooted human teeth were distributed randomly and equally into three groups (n = 10 each) based upon the type of laser irradiation after RCP: Group I (control group) - RCP with ProTaper rotary system using the standard irrigating protocol; Group II - RCP with ProTaper rotary system using the standard irrigating protocol followed by diode laser irradiation; and Group III - RCP with ProTaper rotary system using the standard irrigating protocol followed by Er:YAG laser irradiation. After root sectioning, specimens were dehydrated, then gold plated and observed using a scanning electron microscopy. Then, the smear layer scores were recorded and performed using the statistical analysis. Results: Smear layer removal efficacy of Er:YAG laser was more at coronal, middle, and apical third when compared to Group I and Group II. Debris removal score of Group III (Er:YAG) was better than Group I (17% ethylenediaminetetraacetic acid) and Group II (diode). Conclusion: Er:YAG laser-activated RCP was comparatively efficient in cleaning the smear layer and dentinal tubules opening.


Assuntos
Camada de Esfregaço , Alumínio , Cavidade Pulpar , Dentina , Permeabilidade da Dentina , Érbio , Humanos , Lasers Semicondutores , Microscopia Eletrônica de Varredura , Irrigantes do Canal Radicular , Preparo de Canal Radicular , Ítrio
3.
BMC Oral Health ; 19(1): 274, 2019 12 05.
Artigo em Inglês | MEDLINE | ID: mdl-31805922

RESUMO

BACKGROUND: Dentin hypersensitivity is a frequent finding especially in periodontitis patients. Conventional treatment aims for obstruction of dentin tubules by disabling liquid and osmotic fluctuation to and from the pulpal chamber. A novel bioglass-based desensitizer was shown to obstruct tubules and to resist periodic exposure to lactic acid. Whether this obstruction is resistant to brushing had not been tested so far. Accordingly, the present study aimed to assess dentin tubule obstruction after repeated acid exposure and brushing. METHODS: Sixty dentin discs were cleaned with 17% EDTA, mounted into a pulp fluid simulator and randomly divided into 3 groups: No surface treatment in Group A, Seal&Protect® in group B and DentinoCer in group C. Discs were exposed to 0.1 M non-saturated lactic acid thrice and standardized brushing twice a day for 12 days. At baseline and after 2, 4 and 12 d samples were removed from the setting and prepared for top-view SEM analysis to assess tubule obstruction using the Olley score. Discs were then vertically cut and the section surface morphologically assessed using backscatter imaging. For both vertical and sectional surfaces EDX analysis was used to characterize the surface composition in the tubular and inter-tubular area. RESULTS: Group A showed clean tubular lumina at all time points. From day 2 onwards dentin showed exposed collagen fibers. Group 2 initially showed a complete surface coverage that flattened out during treatment without ever exposing tubules. At baseline, samples of Group C displayed a complete homogeneous coverage. From day 2 on tubules entrances with obstructed lumen became visible. While on day 4 and 12 the dentin surface exposed collagen fibers the lumina remained closed. EDX analysis of the vertical and horizontal views showed that P and Ca were predominant elements in both the inter- and tubular dentin while Si peaks were found in the tubule plugs. CONCLUSION: While group B displayed a packed layer on the surface during the whole investigation time group C samples lost their superficial layer within 48 h. Tubule plugs containing considerable Si proportions indicated previous presence of DentinoCer, while high Ca and P proportions suggest obturation by dentin-like material.


Assuntos
Cerâmica/farmacologia , Dessensibilizantes Dentinários/farmacologia , Permeabilidade da Dentina/efeitos dos fármacos , Sensibilidade da Dentina/terapia , Dentina/efeitos dos fármacos , Materiais Biocompatíveis/farmacologia , Dentina/metabolismo , Sensibilidade da Dentina/metabolismo , Humanos , Microscopia Eletrônica de Varredura
4.
J Contemp Dent Pract ; 20(7): 838-841, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31597805

RESUMO

AIM: The aim of this study is to evaluate the permeability of root dentin after immersion in sodium hypochlorite (NaOCl) at different concentrations for 30 minutes and a final wash with 17% ethylenediaminetetraacetic acid (EDTA) for 3 minutes. MATERIALS AND METHODS: Twenty 1 × 5 mm dentin fragments from the middle third of the root were prepared from 10 bovine teeth and divided into four groups; three of these groups were immersed in 1%, 2.5%, or 5.25% NaOCl for 30 minutes, while group IV was immersed in the saline solution. All dentin fragments were subjected to a final wash with 17% EDTA for 3 minutes. The fluid transport study model was used to measure the fluid conductance induced by hydrostatic pressure. The measurements were repeated twice to confirm the values, and in the case of a discrepancy, the procedure was performed again. RESULTS: The control group (saline solution) presented lower hydraulic conductance mean values with (0.25 ± 0.12). The 5.25% NaOCl followed by 17% EDTA produced the highest mean conductance value (1.18 ± 0.18) followed by 2.5% NaOCl and 17% EDTA with (0.81 ± 0.09) and (0.48 ± 0.02), respectively. The results of the hydraulic conductance evaluation of dentin were different for each NaOCl concentration. After data analyses, there were significant differences from analysis of variance (ANOVA) between all the groups (p < 0.0001). CONCLUSION: The NaOCl solution concentration affects dentin permeability.


Assuntos
Permeabilidade da Dentina , Irrigantes do Canal Radicular , Animais , Bovinos , Cavidade Pulpar , Dentina , Ácido Edético , Hipoclorito de Sódio
5.
Rev. cuba. estomatol ; 56(3): e2075, jul.-set. 2019. tab, graf
Artigo em Espanhol | LILACS | ID: biblio-1093232

RESUMO

RESUMEN Introducción: La permeabilidad dentinaria es conocida como el paso de fluidos, iones, partículas, moléculas y bacterias a través de la dentina bajo ciertas condiciones. Objetivo: Determinar la influencia de la aplicación activa con un aplicador mejorado de un adhesivo dentinario sobre la conductancia hidráulica dentinaria humana, in vitro. Métodos: Estudio experimental in vitro en 40 terceros molares libres de caries, con indicación de exodoncia, provenientes de pacientes sanos entre 15 y 30 años, previo consentimiento informado. Posteriormente se limpiaron, desinfectaron y conservaron para ser incluidos en bloques de resina epóxica. Luego se realizaron cortes transversales y se obtuvieron discos dentinarios de 1,5 mm de espesor y 25 mm de diámetro. Se dividieron en dos grupos de 20 unidades cada uno, se sometieron a grabado con ácido ortofosfórico al 37 por ciento, y se lavaron y secaron. Sobre una balanza de precisión se les aplicó adhesivo dentinario fotopolimerizable, registrando la fuerza ejercida. En el grupo 1 se usó un microbrush en la aplicación y en el grupo 2 un aplicador mejorado diseñado especialmente. Posteriormente cada muestra fue sometida a una cámara de difusión para obtener la tasa de flujo y luego calcular la conductancia hidráulica, la cual otorga información acerca de la permeabilidad de la dentina. Resultados: los resultados obtenidos fueron levemente menores en el grupo 2, sin embargo, experimentaron una distribución normal. Conclusiones: no existen diferencias significativas, al ser analizados estadísticamente bajo diversas pruebas, en los valores de conductancia hidráulica. Tampoco se pudo establecer una relación inversa entre la fuerza de aplicación de un adhesivo en forma activa con un aplicador mejorado y la conductancia hidráulica, por lo cual no se aconseja el uso de dicho instrumento(AU)


ABSTRACT Introduction: Dentin permeability is defined as the passage of fluids, ions, particles, molecules and bacteria through the dentin under certain conditions. Objective: Determine the effect of the active application of a dentin adhesive with an improved applicator on human dentin hydraulic conductance in vitro. Methods: An in vitro experimental study was conducted of 40 caries-free third molars with an indication of exodontia from healthy patients aged 15-30 years, prior informed consent. The molars were cleaned, disinfected and preserved to be included in epoxy resin blocks. Cross-sectional cuts were then made to obtain dentin discs 1.5 mm thick and 25 mm in diameter. These were divided into two 20-unit groups, were subjected to etching with 37 percent orthophosphoric acid, and were washed and dried. On a precision scale photopolymerizable dentin adhesive was applied, recording the force exerted. Application was performed with a Microbrush applicator in Group 1 and with an improved dedicated applicator in Group 2. The samples were then placed in a diffusion chamber to determine flow rate and subsequently estimate hydraulic conductance, thus obtaining information about dentin permeability. Results: Results were slightly lower in Group 2, but they displayed normal distribution. Conclusions: Statistical analysis with various tests did not find any significant differences in hydraulic conductance values, nor could an inverse relation be established between the force of active application of an adhesive with an improved applicator and hydraulic conductance. Therefore, use of that instrument is not advised(AU)


Assuntos
Humanos , Colagem Dentária/métodos , Permeabilidade da Dentina/fisiologia
6.
Dent Mater J ; 38(6): 963-969, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31434831

RESUMO

The aim of this study was to investigate the effects of artificial saliva on permeability measured using a highly sensitive digital flow meter of dentin discs treated with a phosphate containing desensitizer compound (Teethmate desensitizer; TD). Four random groups (n=10) were treated either with TD or distilled water (DW), then stored in artificial saliva (AS) or DW for 1 day, 1 week and 1 month. Flow rates under 2 kPa pressure were calculated as percentage reduction (PR%) from the baseline. The PR% of TD/AS group was significantly lower after 1 day and 1 week, but the PR%s of 1 month groups among TD/AS, TD/DW and DW/AS were not significantly different. The SEM photograph of TD/AS group displayed that the dentin surface was densely covered with mineral deposits. Ca and phosphate ions from the artificial saliva could penetrate into the tubules and precipitate as hydroxyapatite, resulting in the reduction in permeability.


Assuntos
Permeabilidade da Dentina , Sensibilidade da Dentina , Dentina , Humanos , Microscopia Eletrônica de Varredura , Permeabilidade , Saliva Artificial
7.
J Dent ; 86: 53-59, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31125585

RESUMO

OBJECTIVES: To evaluate the performance of calcium/phosphate desensitizing agents when used for dentin permeability and tubule occlusion. METHODS: 1 mm-thick dentin specimens were immersed in 17% EDTA solution and allocated into 7 groups: 1. Clinpro White Varnish, 2. Clinpro XT Varnish, 3. Teethmate Desensitizer, 4. Desensibilize Nano P, 5. Nupro prophylaxis paste, 6. Duraphat (reference product), and 7. Control (no treatment). After treatment, specimens were submitted to erosion-abrasion cycling for 5 days. Dentin permeability was assessed by hydraulic conductance (n = 10) and environmental scanning electron microscopy ESEM (n = 8) post-EDTA, post- treatment and post-cycling. The percentage of permeability (%Lp) was calculated post-treatment and post-cycling. ImageJ software was used to obtain the number of open dentin tubules (ODT) in the micrographs. Data were statistically analyzed (α = 0.05). RESULTS: Post-treatment, the %Lp values of all treatments were significantly lower than the control, with Nupro presenting higher %Lp values than Duraphat. All the groups presented significantly lower %Lp values in the post-treatment in comparison with the post-cycling period, except the control. Post-treatment, all groups showed lower numbers of ODTs than the control, except Nupro. Clinpro WV, Clinpro XT, Duraphat and Nano P presented the best results. Post-cycling, there was no significant difference among groups. Clinpro WV and Duraphat presented lower numbers of ODTs post-treatment than they had post-cycling, and the control had a higher number of ODTs post-treatment than they had post-cycling. CONCLUSIONS: Most treatments were efficient in reducing both dentin permeability and number of ODTs after treatment; however, none of the products were able to resist the erosive-abrasive challenges.


Assuntos
Dessensibilizantes Dentinários , Permeabilidade da Dentina , Sensibilidade da Dentina , Cálcio , Dentina , Humanos , Microscopia Eletrônica de Varredura , Fosfatos
8.
Oper Dent ; 44(5): 530-535, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30951440

RESUMO

This study investigated the effect of conventional and acidified casein phosphopeptide-amorphous calcium phosphate-containing crèmes (CPP-ACP and ACPP-ACP) on dentin permeability (DP) before and after acid challenge, using a fluid-flow measuring device and scanning electron microscopic (SEM) examination. Fifty dentin discs were prepared from intact human third molars. Each tooth was sectioned 3 mm above and below the cementoenamel junction. The smear layer was removed to expose the dentin and dentinal tubules. For fluid-flow measurement, 20 specimens were used and divided into two groups: (a) CPP-ACP (pH=7) and (b) ACPP-ACP (pH=2). The dentin surfaces were treated with the crème for five minutes daily for two weeks, and then an acid challenge was performed using 6% citric acid. Under simulated pulpal pressure, DP was measured at baseline, five minutes, two weeks, and after acid challenge. The remaining specimens were prepared and examined using SEM to investigate dentinal tubule occlusion. DP was not significantly different between the two CPP-ACP-containing crèmes at any test period (p≥0.05). DP after two weeks or acid challenge was significantly lower than that at five minutes (p<0.05). The results were consistent with the SEM micrographs that presented patent dentinal tubules after a five-minute application and partial blockage of dentinal tubules after the two-week application. The dentinal tubules remained partially occluded after acid challenge. Using conventional and acid-modified CPP-ACP-containing crèmes for two weeks decreased DP and partially occluded the dentinal tubules, which resisted acid challenge.


Assuntos
Dessensibilizantes Dentinários , Permeabilidade da Dentina , Fosfatos de Cálcio , Caseínas , Dentina , Humanos , Microscopia Eletrônica de Varredura , Fosfopeptídeos
9.
Braz Oral Res ; 33: e016, 2019 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-30892411

RESUMO

To synthesize Nano eggshell-titanium-dioxide (EB@TiO2) biocomposite and to evaluate its effectiveness in occluding opened dentine tubules. EB@TiO2 was synthesized and characterized using X-ray diffraction (XRD), and Transmission Electron Microscope (TEM). Sixteen simulated bovine dentine discs were prepared and randomly assigned into four groups according to the following treatment (n = 4): Group 1: No treatment; Group 2: eggshell powder; Group 3: EB@TiO2; Group 4: Sensodyne. These were then agitated in a solution of 1g powder and 40mL water for 3hours. Thereafter, each dentine discs from the respective groups were post-treated for 5 min with 2wt% citric acid to test their acid resistant characteristics. Scanning Electron Microscope (SEM) was used to observe the effectiveness of occluded dentine pre-and post-treatment. The cytotoxicity of the synthesized EB@TiO2 was tested using NIH 3T3 assay. ANOVA was used to evaluate the mean values of the occluded area ratio and the data of MTS assay. This was followed by a multi-comparison test with Bonferroni correction (α = .05). The XRD confirmed that EB@TiO2 was successfully modified through ball-milling. The TEM revealed the presence of both spherical and irregular particle shape powders. The SEM result showed that EB@TiO2 could effectively occlude open dentine tubules. Equally, the result demonstrated that EB@TiO2 exhibited the highest acid resistant stability post-treatment. NIH 3T3 assay identified that EB@TiO2 had little effect on the NIH 3T3 cell line even at the highest concentration of 100µg/ml. This study suggests that the application of EB@TiO2 effectively occluded dentine tubules and the occlusion showed a high acid resistant stability.


Assuntos
Dessensibilizantes Dentinários/farmacologia , Permeabilidade da Dentina/efeitos dos fármacos , Sensibilidade da Dentina/terapia , Casca de Ovo/química , Fluoretos/farmacologia , Nanocompostos/química , Nitratos/farmacologia , Fosfatos/farmacologia , Titânio/química , Animais , Bovinos , Combinação de Medicamentos , Casca de Ovo/ultraestrutura , Camundongos , Microscopia Eletrônica de Varredura , Células NIH 3T3 , Nanocompostos/análise , Nanocompostos/uso terapêutico , Titânio/análise , Titânio/farmacologia , Remineralização Dentária
10.
Clin Oral Investig ; 23(2): 899-904, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29948279

RESUMO

OBJECTIVES: To investigate the effects of two disinfection/sterilization methods on the permeability of dentin specimens. MATERIALS AND METHODS: Forty intact human third molars were freshly extracted and cut, close to the pulp chamber, into dentin disks with a 500-µm thickness. The disks were randomized (n = 20 each) into a 70% ethanol group (acid-etched dentin disks soaked in 70% ethanol for 15 min) and a steam autoclaving group (acid-etched dentin disks autoclaved for 25 min). The permeability (Lp) of each dentin disk was measured before and after either treatment using a hydraulic device, and intra- and inter-group differences in values before and after treatment were analyzed using t tests. Field emission scanning electron microscopy (FE-SEM) micrographs of the dentin surface were acquired and examined. FE-SEM samples were prepared using the critical point drying (CPD) method. RESULTS: Immersion in 70% ethanol increased the Lp values of dentin specimens by 17%, which was not statistically significant. Steam autoclaving significantly reduced dentin permeability by 66% because the dentin collagen mesh became compact and collapsed, as detected by FE-SEM. CONCLUSIONS: The disinfection of acid-etched dentin disks using 70% ethanol for 15 min does not significantly affect dentin permeability, whereas sterilization of acid-etched dentin disks via autoclaving significantly reduces dentin permeability. CLINICAL RELEVANCE: Considering the influences of dentin permeability by disinfection/sterilization methods, the disinfection of the acid-etched dentin disks using 70% ethanol for 15 min could be used for the study related to dentin permeability, while the sterilization of autoclaving could not.


Assuntos
Permeabilidade da Dentina , Desinfecção/métodos , Etanol/farmacologia , Condicionamento Ácido do Dente , Permeabilidade da Dentina/efeitos dos fármacos , Temperatura Alta , Humanos , Técnicas In Vitro , Teste de Materiais , Microscopia Eletrônica de Varredura , Dente Serotino , Propriedades de Superfície
11.
Clin Oral Investig ; 23(1): 253-266, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29623418

RESUMO

OBJECTIVES: To synthesize a silver-doped bioactive glass/mesoporous silica nanoparticle (Ag-BGN@MSN), as well as to investigate its effects on dentinal tubule occlusion, microtensile bond strength (MTBS), and antibacterial activity. MATERIALS AND METHODS: Ag-BGN@MSN was synthesized using a modified "quick alkali-mediated sol-gel" method. Demineralized tooth disc models were made and divided into four groups; the following treatments were then applied: group 1-no treatment, group 2-bioglass, group 3-MSN, group 4-Ag-BGN@MSN. Next, four discs were selected from each group and soaked into 6 wt% citric acid to test acid-resistant stability. Dentinal tubule occlusion, as well as the occlusion ratio, was observed using field-emission scanning electron microscopy. The MTBS was also measured to evaluate the desensitizing effect of the treatments. Cytotoxicity was examined using the MTT assay. Antibacterial activity was detected against Lactobacillus casei, and ion dissolution was evaluated using inductively coupled plasma optical emission spectrometry. RESULTS: Ag-BGN@MSN effectively occluded the dentinal tubule and formed a membrane-like layer. After the acid challenge, Ag-BGN@MSN had the highest rate of dentinal tubule occlusion. There were no significant differences in MTBS among the four groups (P > 0.05). All concentrations of Ag-BGN@MSN used had a relative cell viability above 72%. CONCLUSIONS: Ag-BGN@MSN was successfully fabricated using a modified sol-gel method. The Ag-BGN@MSN biocomposite effectively occluded dentinal with acid-resistant stability, did not decrease bond strength in self-etch adhesive system, had low cytotoxicity, and antibacterial effect. CLININAL RELEVANCE: Dentinal tubule sealing induced by Ag-BGN@MSN biocomposite with antibacterial effect is likely to increase long-term stability in DH.


Assuntos
Antibacterianos/química , Cerâmica/química , Dessensibilizantes Dentinários/química , Dentina/efeitos dos fármacos , Dióxido de Silício/química , Prata/química , Antibacterianos/síntese química , Dente Pré-Molar , Ácido Cítrico , Dessensibilizantes Dentinários/síntese química , Permeabilidade da Dentina/efeitos dos fármacos , Sensibilidade da Dentina/tratamento farmacológico , Combinação de Medicamentos , Humanos , Técnicas In Vitro , Teste de Materiais , Microscopia Eletrônica de Varredura , Nanocompostos , Porosidade , Espectrofotometria Atômica , Espectroscopia de Infravermelho com Transformada de Fourier , Resistência à Tração , Difração de Raios X
12.
J Formos Med Assoc ; 118(4): 828-832, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30274828

RESUMO

BACKGROUND/PURPOSE: Stimuli from the oral cavity may penetrate through exposed dentinal tubules and evoke inflammatory pulp response. Anti-bacterial and anti-inflammatory drugs applied to exposed dentin may infiltrate through the dentinal tubules and cause pulp recovery. This study investigated the dentin permeability of anti-bacterial and anti-inflammation drugs via an in-vitro transwell dentin disc tube model. METHODS: Twenty-seven dentin discs prepared from extracted human molars were collected. Nine kinds of drugs were investigated with three dentin discs in each group. These nine drugs included two anti-bacterial drugs (ampicillin sodium and clindamycin phosphate), two corticosteroids (betamethasone sodium phosphate and hydrocortisone sodium succinate), three non-steroidal anti-inflammatory drugs (NSAIDs, piroxicam, lysine acetylsalicylate, and diclofenac sodium), and two natural extracts with anti-inflammatory effect (Ginsenoside Rg1 and Hinokitol). The drugs were introduced to the transwell dentin disc tube model and the 4-hour cumulative release of the drug was detected and recorded by UV-visible spectroscopy. RESULTS: We found that ampicilin sodium had better dentin permeability than clindamycin phosphate. Betamethasone sodium phosphate revealed better dentin permeability than hydrocortisone sodium succinate. Lysine acetylsalicylate showed the best dentin permeability among the three NSAIDs. Ginsenoside Rg1 had the best dentin permeability among the nine drugs tested. However, Hinokitiol could not penetrate the dentin disc after 4 h. CONCLUSION: Regarding the dentin permeability, Ginsenoside Rg1 is the best among the seven anti-inflammatory drugs tested and ampicilin sodium is the better one between the two anti-bacterial drugs tested. Therefore, these two drugs may have high potential for treating exposed dentinal tubule diseases.


Assuntos
Antibacterianos/farmacologia , Anti-Inflamatórios/farmacologia , Permeabilidade da Dentina , Dentina/efeitos dos fármacos , Ampicilina/farmacologia , Aspirina/análogos & derivados , Aspirina/farmacocinética , Betametasona/análogos & derivados , Betametasona/farmacocinética , Ginsenosídeos/farmacologia , Humanos , Lisina/análogos & derivados , Lisina/farmacocinética , Microscopia Eletrônica de Varredura
13.
Dent Mater ; 35(2): 298-309, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30545612

RESUMO

OBJECTIVE: The aim of this study was to evaluate the effect of conditioning solutions containing DL-aspartic amino (Asp) on dentine remineralization induced by bioactive glass 45S5 (BAG) in a simulated oral environment. METHODS: Sixty dentine discs from human third molars were used. Dentine specimens were treated with ethylene diamine tetraacetic acid (EDTA) to create a partially demineralization model and randomly divided to 4 groups: Artificial saliva (AS) group, Asp group (pretreated with Asp and remineralized with distilled water), BAG group (pretreated with distilled water and remineralized by BAG), Asp-BAG group (pretreated with Asp and remineralized by BAG). Each samples were measured at various time points, and at the end of the experiment, 6% citric acid challenge were taken. The remineralization characteristics were analyzed by using the spectroscopic data from attenuated total reflectance spectroscopy (ATR-IR) and Raman spectroscopy. The micro-morphology and structure were characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD). Dentine permeability was measured before and after each treatment to evaluate the resistance of remineralized layer to acid and simulated oral environment. RESULTS: Both BAG and Asp-BAG groups significantly reduced dentine permeability and formed enamel-like apatite layers on dentine surface. For the mineralization of BAG, Asp showed inhibition effect. The 7-day mineral matrix area ratio in BAG group (12.54±2.29) was lower than the value in the Asp-BAG group (17.77±2.27) (p<0.05) and the Raman intensity (RI%) in Asp-BAG Group (1.49±0.26) was also significantly higher than that of BAG group (1.34±0.14) (p<0.05). According to permeability test, the apatite layer in BAG group and Asp-BAG group effectively occluded the dentinal tubules (p<0.05) and had certain acidic resistance (p>0.05). Furthermore, adsorbed acidic amino acid on hydroxyapatite (HAP) altered the crystal to increase into a larger size in diameter during crystal growth. SIGNIFICANCE: The study demonstrated that a superior remineralization efficacy of BAG with Asp pretreatment on dentine.


Assuntos
Biomimética , Remineralização Dentária , Aminoácidos , Dentina , Permeabilidade da Dentina , Humanos , Microscopia Eletrônica de Varredura
14.
BMC Oral Health ; 18(1): 193, 2018 11 21.
Artigo em Inglês | MEDLINE | ID: mdl-30463552

RESUMO

BACKGROUND: Dentine hypersensitivity is especially frequent in patients with pronounced periodontal attachment loss. Aim of the treatment is an obstruction of the dentine tubules in order to inhibit liquid or osmotic motion, which is considered as trigger for pain sensations. Novel approaches aim for obstruction by calcium phosphate compounds in order to rely on biocompatible compounds. It was the aim of the study to optically investigate the morphology and to assess the fluid permeability of treated dentine surfaces. METHODS: Dentine discs were pretreated in an ultrasonic bath with 17% EDTA to clean the lumina of the dentine tubules. Samples of group A remained untreated while Seal&Protect® as a conventional desensitizer was applied for group B and DentinoCer in group C. Discs were mounted into a pulp fluid simulator (PFS) with a methylene blue solution in order to create a flow pressure of 0.5 bar. Over 12 d, discs were exposed three times per day to 0.1 M nonsaturated lactic acid. At baseline and after 2, 8 and 12 d samples were removed from PFS and prepared for SEM analysis. Tubule obstruction was assessed quantitatively using Olley scores and by qualitative description of the surface. Absorption spectrometry was used to assess the concentration of leaked methylene blue outside the samples in order to estimate dentine permeability. RESULTS: Untreated discs showed clean lumina of all tubules at all time points and magnifications. From day 2 onwards dentine showed exposed collagene fibers due to acid exposition. Seal&Protect® initially showed homogenous dentine surface coverage that got a more granulomatous aspect in the course of treatment time. Few samples showed sporadic tubules with open lumen at day 8 and 12. Group C showed samples with a homogeneous, even surface. Narrow slits in the superficial layer are visible from day 4 on, but the dentine surface remained invisible and dentine tubules were closed till the end of the investigation period. CONCLUSION: Over 12 d of lactid acid exposure, samples showed complete coverage of the dentine tubules in the chosen in-vitro-model when treated with Seal&Protect® or DentinoCer.


Assuntos
Cerâmica/farmacologia , Dessensibilizantes Dentinários/farmacologia , Permeabilidade da Dentina/efeitos dos fármacos , Sensibilidade da Dentina/terapia , Dentina/efeitos dos fármacos , Animais , Materiais Biocompatíveis/farmacologia , Bovinos , Dentina/diagnóstico por imagem , Dentina/metabolismo , Sensibilidade da Dentina/metabolismo , Técnicas In Vitro , Ácido Láctico/farmacologia
15.
Biomed Res Int ; 2018: 4072815, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30276206

RESUMO

The effectiveness of remineralizing agents in reducing dentine permeability by tubule occlusion using fluid filtration device functioning at 100 cmH2O (1.4 psi) pressure and SEM/EDX analysis were evaluated and compared. Seventy (n = 70) dentine discs of 1±0.2 mm width were prepared from sound permanent human molars. Fifty (n = 50) dentine discs were randomly divided into 5 groups (n = 10): Group 1: GC Tooth Mousse Plus (Recaldent GC Corporation Tokyo, Japan), Group 2: Clinpro™ White Varnish (3M ESPE, USA), Group 3: Duraphat® Varnish (Pharbil Waltrop GmbH, Germany), Group 4: Colgate Sensitive Pro-Relief™ dentifrice (Colgate Palmolive, Thailand), and Group 5: Biodentine™ (Septodont/UK). Dentine permeability was measured after treatment application at 10 minutes, artificial saliva immersion at 7 days, and citric acid challenge for 3 minutes. Data were analyzed by two-way repeated measures ANOVA. Dentine specimens (n = 20) were used for SEM/EDX analyses to obtain qualitative results on dentine morphology and surface deposits. Each treatment agent significantly reduced dentine permeability immediately after treatment application and created precipitates on treated dentine surfaces. All agents increased permeability values after 7 days of artificial saliva immersion except Clinpro White Varnish and Biodentine. Clinpro White Varnish exhibited significant resistance to acid challenge compared to others. Colgate Sensitive Pro-Relief dentifrice has a dual mechanism of action in reducing the dentine sensitivity.


Assuntos
Dessensibilizantes Dentinários/farmacologia , Permeabilidade da Dentina , Humanos , Microscopia Eletrônica de Varredura , Dente Molar
16.
Niger J Clin Pract ; 21(10): 1284-1290, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30297560

RESUMO

Objective: The objective of this study is to compare the effectiveness of final irrigation with chitosan, ethylenediaminetetraacetic acid (EDTA), and citric acid (CA) on a resin-based sealer (AH plus sealer [Dentsply DeTrey, Konstanz, Germany]) penetration into dentinal tubules using confocal laser scanning microscopy. Materials and Methods: Seventy recently extracted human mandibular premolars were instrumented and irrigated with sodium hypochlorite (NaOCl), then divided into four groups according to the final irrigation regimen used: (1) the EDTA group: 17% EDTA + 2.5% NaOCl, (2) the CA group: 10% CA + 2.5% NaOCl, (3) the chitosan group: 0.2% chitosan + 2.5% NaOCl, and (4) the control group: 2.5% NaOCl. All teeth were obturated using the cold lateral condensation technique with gutta-percha and AH Plus sealer labeled with fluorescent dye. The apical 2 mm of specimen was discarded, and slices were obtained for apical, middle, and coronal thirds of the root with 1 mm intervals. Maximum, mean, and percentage of sealer penetration (SP) inside tubules were measured using confocal laser scanning microscopy. Results: The percentage of SP was significantly higher in chitosan, EDTA, and CA group than control group for coronal thirds (P < 0.05), whereas there was no significant difference among all groups for middle and apical thirds. Chitosan and EDTA showed increased mean values of SP depth for middle thirds (P < 0.05). In all sections, the maximum depth of SP was significantly lower in EDTA group than other groups (P < 0.05). Conclusions: Chitosan, EDTA, and CA significantly improved the percentage of SP for coronal thirds.


Assuntos
Quitosana/farmacologia , Ácido Cítrico/farmacologia , Permeabilidade da Dentina/efeitos dos fármacos , Dentina/diagnóstico por imagem , Ácido Edético/farmacologia , Microscopia Confocal , Irrigantes do Canal Radicular/farmacologia , Dente Pré-Molar , Dentina/metabolismo , Resinas Epóxi , Humanos , Materiais Restauradores do Canal Radicular , Preparo de Canal Radicular/métodos , Hipoclorito de Sódio/farmacologia
17.
Arch Oral Biol ; 96: 130-136, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30237094

RESUMO

OBJECTIVE: To evaluate dentin permeability and tubule occlusion of in-office desensitizing treatments, and to analyze their resistance to erosive/abrasive challenges. DESIGN: Ninety-one 1mm-thick dentin discs were immersed in EDTA solution for 5 min. After analyzing the maximum dentin permeability, the specimens were randomly allocated into 7 experimental groups (n = 10): Control (no treatment); Er,Cr:YSGG laser; Nd:YAG laser; Gluma Desensitizer; Duraphat; Pro-Argin toothpaste; Calcium Sodium Phosphosilicate (CSP) paste. The post-treatment permeability was assessed and then the specimens were subjected to a 5-day erosion-abrasion cycling protocol: 4x/day of immersion in citric acid solution (5 min;0.3%), followed by exposure to clarified human saliva (60 min). After the first and last acid challenges, specimens were brushed for 15 s, with exposure to the toothpaste slurry for total time of 2 min. Dentin permeability was re-measured (post-cycling). Percentage of dentin permeability for each experimental time was calculated in relation to the maximum permeability (%Lp). Data were analyzed with 2-way repeated measures ANOVA and Tukey tests (α = 0.05). Surface modifications were analyzed by scanning electron microscopy. RESULTS: In both experimental time CSP paste and Gluma Desensitizer did not differ from each other (p = 0.0874), and were the only groups that presented significantly lower %Lp than the Control (p = 0.026 and p = 0.022, respectively). After treatment, they were able to reduce dentin permeability in 82% and 72%, respectively. The %Lp post-cycling was higher than post-treatment value for all groups (p = 0.008). Dentin permeability increased 21% for CSP paste and 12% for Gluma, but they remained significant different from Control. Deposits on the surface were observed for CSP paste; and for Gluma, tubule diameters were shown to be smaller. CONCLUSIONS: CSP paste and Gluma Desensitizer were the only treatments able to decrease dentin permeability post-treatment and to sustain low permeability post-cycling.


Assuntos
Dessensibilizantes Dentinários/farmacologia , Sensibilidade da Dentina/prevenção & controle , Escovação Dentária , Ácido Cítrico , Permeabilidade da Dentina/efeitos dos fármacos , Glutaral , Técnicas In Vitro , Teste de Materiais , Metacrilatos , Microscopia Eletrônica de Varredura , Saliva , Propriedades de Superfície
18.
Oral Health Prev Dent ; 16(4): 339-344, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30175331

RESUMO

PURPOSE: To evaluate the efficacy of Clinpro XT in reducing dentin permeability and the stability of this effect after different acid challenges. MATERIALS AND METHODS: Sixty-five roots of extracted human third molars were used. From each tooth, one dentin specimen was prepared and connected to a fluid filtration system to measure the dentin permeability after each of the following steps: sample preparation; treatment with 37% phosphoric acid; application of Clinpro XT; three acid challenges. Specimens were randomly assigned to 5 groups (n = 13) according to the acidic solution applied: Coca-Cola, natural lemon juice, wine vinegar, white wine and Red Bull energy drink. An additional 10 third molars were used to evaluate the degree of occlusion of the dentinal tubules and the surface roughness. RESULTS: Clinpro XT statistically significantly reduced dentin permeability after just a single application. No statistically significant increase in dentin permeability could be detected after three consecutive challenges. The application of Clinpro XT promotes the occlusion of dentinal tubules and reduces the surface roughness. CONCLUSION: The Clinpro XT is effective in reducing dentin permeability. This effect persists even after acid challenges.


Assuntos
Resinas Compostas , Permeabilidade da Dentina/efeitos dos fármacos , Dentina/efeitos dos fármacos , Selantes de Fossas e Fissuras , Ácidos/efeitos adversos , Bebidas/efeitos adversos , Dentina/ultraestrutura , Humanos , Microscopia Eletrônica de Varredura , Erosão Dentária/induzido quimicamente
19.
Dent Mater J ; 37(6): 919-927, 2018 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-29998943

RESUMO

The purpose of this study was to evaluate the effect of fluoride mouthrinses on dentin bonding performance of a two-step selfetch adhesive; Clearfil SE Bond. Bovine root dentin surface was treated with either 450, 900, or 9,000 ppm F solutions for 30 s (immediate), and continually treated for one month (one month) before the bonding procedures. Microtensile bond strength (µTBS) test and scanning electron microscopic (SEM) observation of the acid-base resistance zone (ABRZ) were performed. Chemical state of fluorine on dentin surface was analyzed by X-ray absorption fine structure (XAFS). The 450 and 900 ppm F fluoride mouthrinses did not influence the µTBS to dentin, while the 9,000 ppm F fluoride solution adversely affected the µTBSs. The fluoride application to dentin significantly enhanced acid resistance at the adhesive/dentin interface including the ABRZ. The XAFS analysis indicated different concentrations of fluoride might create different chemical compounds on the dentin surface, influencing the µTBS results.


Assuntos
Cariostáticos/química , Colagem Dentária , Adesivos Dentinários/química , Fluoretos Tópicos/química , Antissépticos Bucais/química , Cimentos de Resina/química , Raiz Dentária/efeitos dos fármacos , Condicionamento Ácido do Dente , Animais , Bovinos , Análise do Estresse Dentário , Permeabilidade da Dentina , Teste de Materiais , Microscopia Eletrônica de Varredura , Propriedades de Superfície , Resistência à Tração , Espectroscopia por Absorção de Raios X
20.
Lasers Med Sci ; 33(9): 1883-1890, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29850962

RESUMO

The purpose of this in vitro study was to evaluate and compare the efficacy of erbium-doped yttrium aluminum garnet (Er:YAG) laser, neodymium-doped yttrium aluminum garnet (Nd:YAG) laser, PrevDent nano-hydroxyapatite toothpaste plus Repairing Serum Kit (PNH), and NUPRO Sensodyne Prophylaxis Paste with NovaMin (NPP) on dentin permeability reduction. Forty dentin discs obtained from bovine incisors were divided into four study groups: Er:YAG laser-treated (2940 nm; 0.2 W, 80 mJ/pulse, 3 Hz); Nd:YAG laser-treated (1064 nm; 1 W, 10 Hz); PNH-treated; and NPP-treated groups. The quantitative changes in permeability of each dentin disc were measured using a computerized fluid filtration method (CFFM) before and after desensitizer treatments. The data were analyzed using the Wilcoxon, paired-samples t, Kruskal-Wallis, and Mann-Whitney U tests. The dentin surfaces and tubules were also morphologically detected by scanning electron microscopy (SEM). In all groups, dentin permeability was significantly reduced after the desensitizer and laser treatments (p < 0.05). Among the groups, we detected a significant difference in only when comparing the Er:YAG laser- and NPP-treated groups (p = 0.034). SEM analysis revealed physical changes in the dentin surface in all groups. This in vitro study shows that all tested desensitizers and laser treatments reduced dentin permeability. Also, surface changes, such as complete or partial occlusion or shrinkage of dentin tubules, were observed in all groups. Although the laser groups performed best, the PNH protocol can be considered as an alternative therapeutic product. In addition, clinical and laboratory studies should be performed for this product, and their efficacy should be assessed by combined therapy with lasers.


Assuntos
Dessensibilizantes Dentinários/farmacologia , Permeabilidade da Dentina , Lasers de Estado Sólido , Animais , Bovinos , Dentina/efeitos dos fármacos , Dentina/ultraestrutura , Permeabilidade da Dentina/efeitos dos fármacos , Combinação de Medicamentos , Durapatita/farmacologia , Fluoretos/farmacologia , Vidro , Nanopartículas/química , Nitratos/farmacologia , Fosfatos/farmacologia
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