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1.
PLoS One ; 15(5): e0233057, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32396545

RESUMO

Poor survival of human pluripotent stem cells (hPSCs) following freezing, thawing, or passaging hinders the maintenance and differentiation of stem cells. Rho-associated kinases (ROCKs) play a crucial role in hPSC survival. To date, a typical ROCK inhibitor, Y-27632, has been the primary agent used in hPSC research. Here, we report that another ROCK inhibitor, fasudil, can be used as an alternative and is cheaper than Y-27632. It increased hPSC growth following thawing and passaging, like Y-27632, and did not affect pluripotency, differentiation ability, and chromosome integrity. Furthermore, fasudil promoted retinal pigment epithelium (RPE) differentiation and the survival of neural crest cells (NCCs) during differentiation. It was also useful for single-cell passaging of hPSCs and during aggregation. These findings suggest that fasudil can replace Y-27632 for use in stem research.


Assuntos
1-(5-Isoquinolinasulfonil)-2-Metilpiperazina/análogos & derivados , Células-Tronco Pluripotentes/citologia , Células-Tronco Pluripotentes/efeitos dos fármacos , Quinases Associadas a rho/antagonistas & inibidores , 1-(5-Isoquinolinasulfonil)-2-Metilpiperazina/farmacologia , Amidas/farmacologia , Diferenciação Celular/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células-Tronco Embrionárias/citologia , Células-Tronco Embrionárias/efeitos dos fármacos , Humanos , Células-Tronco Pluripotentes Induzidas/citologia , Células-Tronco Pluripotentes Induzidas/efeitos dos fármacos , Crista Neural/citologia , Crista Neural/efeitos dos fármacos , Inibidores de Proteínas Quinases/farmacologia , Piridinas/farmacologia , Epitélio Pigmentado da Retina/citologia , Epitélio Pigmentado da Retina/efeitos dos fármacos , Pesquisa com Células-Tronco
2.
Cell Stem Cell ; 26(5): 627-628, 2020 05 07.
Artigo em Inglês | MEDLINE | ID: mdl-32348728

RESUMO

COVID-19 has unfortunately halted lab work, conferences, and in-person networking, which is especially detrimental to researchers just starting their labs. Through social media and our reviewer networks, we met some early-career stem cell investigators impacted by the closures. Here, they introduce themselves and their research to our readers.


Assuntos
Pesquisa Biomédica , Infecções por Coronavirus , Relações Interprofissionais , Pandemias , Pneumonia Viral , Pesquisa com Células-Tronco , Betacoronavirus , Pesquisa Biomédica/tendências , Mobilidade Ocupacional , Humanos
4.
World Neurosurg ; 134: e405-e411, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31655231

RESUMO

OBJECTIVE: Although the overall publication trends in Parkinson disease (PD) and characteristics of top-cited articles have been reported, there was only 1 literature analysis published in 2012 with a special focus on stem cells. It is necessary to evaluate and update the global publication trends in stem cell research of PD. METHODS: We identified the publications designated as "article" about stem-cell research of PD between 1999 and 2018 in the Web of Science Core Collection. We used HistCite to analyze annual outputs, journals, countries/regions, and institutions every 5 years and visualized global collaborations between publications by VOSviewer. Moreover, to track the growing hotspots, MeSH terms of each publication were obtained by Medical Text Indexer according to the title and abstract. RESULTS: We described the publication trends and topic hotspots of stem-cell research of PD by bibliometric analysis of 1709 papers. Researchers showed growing interest in publishing relevant scientific literature in journals associated with stem cells or multidisciplinary science. Stem cell research of PD was more common in developed countries and regions. The United States of America was the most contributive country throughout, accounting for 33% of total publications and ranking first in all 5-year periods. Harvard University was the most productive institution in this area, ranking first during 1999-2003, 2004-2008, and 2009-2013. The application of induced pluripotent stem cells was at the forefront of cell therapies for PD. CONCLUSIONS: These bibliometric findings suggest that stem cell research consistently promotes the understanding and treatment of PD.


Assuntos
Bibliometria , Pesquisa Biomédica/tendências , Doença de Parkinson/terapia , Pesquisa com Células-Tronco , Pesquisa Biomédica/métodos , Eficiência , Humanos , Doença de Parkinson/epidemiologia
6.
Exp Hematol ; 80: 1-10, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31765673

RESUMO

The processes generating cells of adaptive immunity render them less amenable to the single cytokine signals used so effectively to regenerate myeloid cells. T-cell neogenesis begins in the bone marrow, where specific sets of late osteolineage cells govern the specification of hematopoietic cells capable of migrating to the thymus where differentiation is completed. Osteocalcin-expressing bone marrow stromal cells producing Dll4 serve as a progenitor niche enabling this T-competent cell production. Biocompatible alginate-based cryogels containing bone morphogenetic proteins (BMP-2) and the Notch ligand Dll4 were engineered to recapitulate the endogenous niche. These cryogels are highly pliable and can be injected under the skin of animals undergoing bone marrow transplantation. The result in mice is an ectopic niche fostering T-competent progenitor generation that results in improved T-cell numbers and receptor diversity. The recipients can generate neoantigen vaccine responses while having improved tolerance manifest by reduced graft-versus-host disease upon allogeneic transplant. Through emerging details of niches in the bone marrow, therapeutics more complex than those necessary for myeloid reconstitution are possible. Niche biology-guided bioengineered design offers the possibility of regenerative therapies for T lymphoid cells.


Assuntos
Engenharia Celular/métodos , Linfopoese , Medicina Regenerativa/métodos , Nicho de Células-Tronco/fisiologia , Subpopulações de Linfócitos T/citologia , Proteínas Adaptadoras de Transdução de Sinal/farmacologia , Adulto , Fatores Etários , Alginatos , Animais , Atrofia , Austrália , Distinções e Prêmios , Medula Óssea/fisiologia , Proteína Morfogenética Óssea 2/farmacologia , Proteínas de Ligação ao Cálcio/farmacologia , Linhagem da Célula , Criogéis/farmacologia , Xenoenxertos , Humanos , Camundongos , Osteocalcina/biossíntese , Sociedades Científicas , Pesquisa com Células-Tronco , Subpopulações de Linfócitos T/transplante , Timo/patologia
8.
EMBO J ; 38(21): e103331, 2019 10 04.
Artigo em Inglês | MEDLINE | ID: mdl-31602659

RESUMO

Research that uses stem cell-based chimeras promises to advance our understanding of human developmental biology, as well as new medical interventions, such as generating transplantable human organs in livestock. However, along with these exciting research possibilities come moral concerns about the moral humanization of animals, especially when it comes to the potential effects of human cells in the brains of experimental animals. Recent work involving neurologically chimeric mice may suggest that such worries are reasonable. However, this overlooks the crucial social and neurological conditions for enabling the development of conscious self-awareness, the absence of which leaves us only with animal welfare to monitor and consider.


Assuntos
Quimera/metabolismo , Modelos Animais , Modelos Neurológicos , Pesquisa com Células-Tronco/ética , Bem-Estar do Animal , Animais , Humanos , Camundongos
9.
Rom J Morphol Embryol ; 60(2): 723-728, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31658351

RESUMO

Medicine is an ever-changing science. Stem cell research is nowadays part of the medicine. After developments and trials for decades, in 1988 it was announced that a variety of diseases and injuries would be cured with new stem cell therapy, such as: cancer, diabetes, Parkinson's, spinal cord injuries and many others. After almost 10 years of research in the field, in 2007 other good news and hopes were announced: the possibility to create induced pluripotent stem cells, derived from somatic cells, easily used to establish any disease-specific cell line. And research is going on. In order to find answer to a variety of challenges in this area, a researcher faces the following main question: Which are the legislations and the normative standards to be taken into account when we are supposing to conduct a research on/with stem cells? The purpose of the paper is: (i) to familiarize professionals with the current steps in the evolution of stem cell research; (ii) to provide main legal orientations related to stem cell research; (iii) to indicate limits and explanations on legal regulations related to the research on stem cells.


Assuntos
Células-Tronco Pluripotentes Induzidas/metabolismo , Pesquisa com Células-Tronco/legislação & jurisprudência , Humanos
10.
Cell Tissue Bank ; 20(4): 467-488, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31606767

RESUMO

Breastmilk is a dynamic, multi-faceted, and complex fluid containing a plethora of biochemical and cellular components that execute developmental effects or differentiation program, providing nourishment and immunity to newborns. Recently, it was reported that breastmilk contains a heterogeneous population of naïve cells, including pluripotent stem cells, multipotent stem cells, immune cells, and non-immune cells. The stem cells derived from breastmilk possess immune privilege and non-tumorigenic properties. Thus, breastmilk may represent an ideal source of stem cells collected by non-perceive procedure than other available sources. Thus, this "maternally originating natural regenerative medicine" may have innumerable applications in clinical biology, cosmetics, and pharmacokinetics. This review describes the efficient integrated cellular system of mammary glands, the impressive stem cell hierarchy of breastmilk, and their possible implications in translational research and therapeutics.


Assuntos
Leite Humano/citologia , Células-Tronco Multipotentes/citologia , Células-Tronco Pluripotentes/citologia , Pesquisa com Células-Tronco , Diferenciação Celular , Humanos , Imunidade Celular , Recém-Nascido , Glândulas Mamárias Humanas/citologia , Glândulas Mamárias Humanas/crescimento & desenvolvimento , Glândulas Mamárias Humanas/imunologia , Leite Humano/imunologia , Células-Tronco Multipotentes/imunologia , Células-Tronco Pluripotentes/imunologia , Medicina Regenerativa/métodos
12.
PLoS One ; 14(8): e0221164, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31454364

RESUMO

Knock-in (KI) gene targeting can be employed for a wide range of applications in stem cell research. However, vectors for KI require multiple complicated processes for construction, including multiple times of digestion/ligation steps and extensive restriction mapping, which has imposed limitations for the robust applicability of KI gene targeting. To circumvent this issue, here we introduce versatile and systematic methods for generating KI vectors by molecular cloning. In this approach, we employed the Multisite Gateway technology, an efficient in vitro DNA recombination system using proprietary sequences and enzymes. KI vector construction exploiting these methods requires only efficient steps, such as PCR and recombination, enabling robust KI gene targeting. We show that combinatorial usage of the KI vectors generated using this method and site-specific nucleases enabled the precise integration of fluorescent protein genes in multiple loci of human and common marmoset (marmoset; Callithrix jacchus) pluripotent stem cells. The methods described here will facilitate the usage of KI technology and ultimately help to accelerate stem cell research.


Assuntos
DNA Recombinante/genética , Técnicas de Introdução de Genes/métodos , Marcação de Genes/métodos , Vetores Genéticos/genética , Animais , Callithrix/genética , Clonagem Molecular/métodos , Desoxirribonucleases/genética , Genes Reporter/genética , Proteínas de Fluorescência Verde/genética , Humanos , Pesquisa com Células-Tronco
16.
17.
Nat Cell Biol ; 21(7): 801-811, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31209293

RESUMO

Despite many reports of putative stem-cell-based treatments in genetic and degenerative disorders or severe injuries, the number of proven stem cell therapies has remained small. In this Review, we survey advances in stem cell research and describe the cell types that are currently being used in the clinic or are close to clinical trials. Finally, we analyse the scientific rationale, experimental approaches, caveats and results underpinning the clinical use of such stem cells.


Assuntos
Terapia Baseada em Transplante de Células e Tecidos , Células-Tronco Embrionárias/transplante , Terapia Genética , Regeneração/genética , Células da Medula Óssea/metabolismo , Terapia Baseada em Transplante de Células e Tecidos/métodos , Terapia Genética/métodos , Humanos , Regeneração/fisiologia , Pesquisa com Células-Tronco
18.
Neurology ; 93(6): 252-258, 2019 08 06.
Artigo em Inglês | MEDLINE | ID: mdl-31227615

RESUMO

OBJECTIVE: To characterize the marketplace for direct-to-consumer (DTC) unproven stem cell-based interventions (SCBI) for neurologic diseases and injuries using crowdfunding data. METHODS: Search terms were developed from previous empirical studies of DTC businesses and the International Classification of Diseases-11 for neurologic diseases and used to query GoFundMe's internal search engine. Campaigns initiated November 2017-2018 and seeking SCBI for neurologic diseases and injuries (n = 1,030) were reviewed to identify the number of donors, number of Facebook shares, recipient location, funding pledged, funding requested, underlying neurologic condition, treatment location, and treatment facility name. RESULTS: A total of 1,030 crowdfunding campaigns for SCBI for neurologic diseases and injuries requested $33,449,979 and received $5,057,069 from 38,713 donors. The most common neurologic condition identified was multiple sclerosis (MS) (n = 404, 35.5%). Of campaigns naming specific destination facilities (n = 392), the most common clinical settings identified were the Stem Cell Institute in Panama City, Panama (n = 91, 23.2%), StemGenex in San Diego, California (n = 44, 11.2%), and Clinica Ruiz in Puebla, Mexico (n = 36, 9.2%). CONCLUSIONS: MS dominated the total number of crowdfunding campaigns. Most campaigns were linked to individuals from regions geographically proximal to destination facilities advertising SCBI for particular neurologic diseases. Most of the clinical destinations were located in comparatively high-income countries such as the United States, Mexico, and Panama. These findings provide considerable insight into the DTC marketplace for SCBI. Analysis of crowdfunding campaigns can be used to develop more targeted patient education initiatives and health policies related to domestic and international travel for unproven SCBI.


Assuntos
Crowdsourcing/economia , Doenças do Sistema Nervoso/terapia , Pesquisa com Células-Tronco/economia , Traumatismos do Sistema Nervoso/terapia , Humanos , Esclerose Múltipla/terapia , Ferramenta de Busca , Mídias Sociais
20.
Curr Stem Cell Res Ther ; 14(2): 80-82, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30957721

RESUMO

This article presents a special issue of "Current Stem Cell Research & Therapy" devoted to exploring and exploiting tissue engineering through the design of multifunctional therapeutic systems. This lead article draws from twelve contributed articles to discuss the most recent advancements in this emerging field. The common theme in the contributed articles is the emerging therapeutic strategies, and a special appeal is made for collaboration between engineers and biologists for the development of multifunctional therapeutic systems for tissue engineering and regenerative medicine.


Assuntos
Regeneração Óssea/genética , Sistemas de Liberação de Medicamentos , Nanotecnologia/tendências , Engenharia Tecidual/tendências , Materiais Biocompatíveis/uso terapêutico , Humanos , Medicina Regenerativa/tendências , Pesquisa com Células-Tronco , Tecidos Suporte/tendências
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