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1.
Int J Mol Sci ; 22(16)2021 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-34445416

RESUMO

The antioxidant and anti-inflammatory potentials of polyphenols contained in Gynura procumbens (GP) extract were systematically analyzed. Polyphenols in GP were analyzed for nine peaks using high-performance liquid chromatography (HPLC) combined with mass spectrometry (MS), and quantitatively determined through each standard. A total of nine polyphenolic compounds were identified in the samples and their MS data were tabulated. To determine the potential of bioactive ingredients targeting DPPH and COX-2, we analyzed them by ultrafiltration combined with LC. The results identified the major compounds exhibiting binding affinity for DPPH and COX-2. Caffeic acid, kynurenic acid, and chlorogenic acid showed excellent binding affinity to DPPH and COX-2, suggesting that they can be considered as major active compounds. Additionally, the anti-inflammatory effect of GP was confirmed in vitro. This study will not only be used to provide basic data for the application of GP to the food and pharmaceutical industries, but will also provide information on effective screening methods for other medicinal plants.


Assuntos
Anti-Inflamatórios/análise , Antioxidantes/análise , Asteraceae/química , Ciclo-Oxigenase 2/metabolismo , Polifenóis/análise , Animais , Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Compostos de Bifenilo/metabolismo , Cromatografia Líquida de Alta Pressão , Avaliação Pré-Clínica de Medicamentos , Humanos , Ligantes , Lipopolissacarídeos/efeitos adversos , Espectrometria de Massas , Camundongos , Picratos/metabolismo , Extratos Vegetais/química , Polifenóis/farmacologia , Células RAW 264.7
2.
Artigo em Inglês | MEDLINE | ID: mdl-34339956

RESUMO

Ylang-ylang (YY) essential oil (EO) is distilled from the fresh-mature flowers of the Annonaceae family tropical tree Cananga odorata [Lam.] Hook. f. & Thomson, and is widely used in perfume and cosmetic industries for its fragrant character. Herein, two different metabolomic profiles obtained using high-performance thin-layer chromatography (HPTLC), applying different stains, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH·) and p-anisaldehyde, were used for discrimination of 52 YY samples across geographical origins and distillation grades. The first profile is developed using the DPPH· stain based on the radical scavenging activity (RSA) of YY EOs. Results of the HPTLC-DPPH· assay confirmed that RSA of YY EOs is in proportion to the length of distillation times. Major components contributing to the RSA of YY EOs were tentatively identified as germacrene D and α-farnesene, eugenol and linalool, by gas chromatography-mass spectrometry (GC-MS) and GC-flame ionisation detector (GC-FID). The second profile was developed using the general-purpose p-anisaldehyde stain based on the general chemical composition of YY EOs. Untargeted metabolomic discrimination of YY EOs from different geographical origins was performed based on the HPTLC-p-anisaldehyde profiles, followed by principal component analysis (PCA). A discrimination and prediction model for identification of YY distillation grade was developed using PCA and partial least squares regression (PLS) based on binned HPTLC-ultraviolet (254 nm) profiles, which was successfully applied to distillation grade determination of blended YY Complete EOs.


Assuntos
Cananga/química , Cromatografia em Camada Delgada/métodos , Sequestradores de Radicais Livres/química , Óleos Voláteis/química , Óleos Vegetais/química , Compostos de Bifenilo/análise , Compostos de Bifenilo/metabolismo , Cromatografia Líquida de Alta Pressão , Destilação , Eugenol/análise , Eugenol/química , Eugenol/metabolismo , Sequestradores de Radicais Livres/metabolismo , Metabolômica , Análise Multivariada , Óleos Voláteis/metabolismo , Picratos/análise , Picratos/metabolismo , Óleos Vegetais/metabolismo , Sesquiterpenos/análise , Sesquiterpenos/química , Sesquiterpenos/metabolismo
3.
Molecules ; 26(12)2021 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-34200647

RESUMO

Curcuminoids are the main bioactive components of the well-known Asian spice and traditional medicine turmeric. Curcuminoids have poor chemical stability and bioavailability; in vivo they are rapidly metabolized to a set of bioreduced derivatives and/or glucuronide and sulfate conjugates. The reduced curcuminoid metabolites were also reported to exert various bioactivities in vitro and in vivo. In this work, we aimed to perform a comparative evaluation of curcuminoids and their hydrogenated metabolites from a medicinal chemistry point of view, by determining a set of key pharmacokinetic parameters and evaluating antioxidant potential in relation to such properties.Reduced metabolites were prepared from curcumin and demethoxycurcumin through continuous-flow hydrogenation. As selected pharmacokinetic parameters, kinetic solubility, chemical stability, metabolic stability in human liver microsomes, and parallel artificial membrane permeability assay (PAMPA)-based gastrointestinal and blood-brain barrier permeability were determined. Experimentally determined logP for hydrocurcumins in octanol-water and toluene-water systems provided valuable data on the tendency for intramolecular hydrogen bonding by these compounds. Drug likeness of the compounds were further evaluated by a in silico calculations. Antioxidant properties in diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and oxygen radical absorbance capacity (ORAC) assays were comparatively evaluated through the determination of ligand lipophilic efficiency (LLE). Our results showed dramatically increased water solubility and chemical stability for the reduced metabolites as compared to their corresponding parent compound. Hexahydrocurcumin was found the best candidate for drug development based on a complex pharmacokinetical comparison and high LLE values for its antioxidant properties. Development of tetrahydrocurcumin and tetrahydro-demethoxycurcumin would be limited by their very poor metabolic stability, therefore such an effort would rely on formulations bypassing first-pass metabolism.


Assuntos
Antioxidantes/farmacologia , Antioxidantes/farmacocinética , Diarileptanoides/farmacologia , Diarileptanoides/farmacocinética , Disponibilidade Biológica , Compostos de Bifenilo/metabolismo , Permeabilidade da Membrana Celular/fisiologia , Química Farmacêutica , Curcuma/metabolismo , Curcumina/análogos & derivados , Curcumina/metabolismo , Glucuronídeos/metabolismo , Humanos , Hidrogenação , Microssomos Hepáticos/metabolismo , Picratos/metabolismo , Solubilidade
4.
Acta Sci Pol Technol Aliment ; 20(3): 253-263, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34304544

RESUMO

BACKGROUND: The diet of most of the population is limited to a reduced number of plants, even in areas that have a varied and extensive diversity, such as Brazil. Unconventional Food Plants (UFPs) are plants considered exotic, native, and wild that grow naturally and can be used as food. Among these is Talinum paniculatum (Jacq.) Gaertn., which is widespread throughout Brazil and can be a potential source of nutrients. Due to the potential of utilization of UFPs in human food and the lack of studies regarding the composition of T. paniculatum, this study aimed to assess the nutritional value of T. paniculatum leaves, their antioxidant capacity, and their antimicrobial activity for possible use in food. METHODS: The characterization of the leaves of T. paniculatum was carried out through analyses of proximal composition, color, ascorbic acid, mineral profile, and antinutritional factors showing the presence of condensed and hydrolysable tannins and nitrates in low concentrations. Solvents of water, ethanol, ethanol/water, methanol, methanol/water, methanol/acetic acid and acetone/water/acetic acid were used to evaluate the extraction yield of phenolic compounds, antioxidant capacity, and antibacterial activity of the extracts. RESULTS: High contents of protein (18.61 g 100 g-1), insoluble dietary fiber (34.75 g 100 g-1), ascorbic acid (81.03 mg 100 g-1), magnesium, potassium, and calcium (649.600, 411.520 and 228.117 mg 100 g-1, respectively) were observed. Extraction using the mixture of solvents of methanol/acetic acid showed the highest yield of phenolic compounds (432.73 mg EAG 100 g-1) and antioxidant capacity using the DPPH assay (3144.92 mg 100 g-1). Bacillus cereus growth was inhibited by the T. paniculatum extracts. CONCLUSIONS: T. paniculatum leaves are a source of nutrients and their extracts have antioxidant and antibacterial potentials which can be used as supplements in food to improve one's health.


Assuntos
Antibacterianos/farmacologia , Antioxidantes/farmacologia , Caryophyllales/química , Nutrientes/análise , Valor Nutritivo , Extratos Vegetais/farmacologia , Folhas de Planta/química , Antibacterianos/análise , Antioxidantes/análise , Bacillus cereus/efeitos dos fármacos , Bacillus cereus/crescimento & desenvolvimento , Compostos de Bifenilo/metabolismo , Brasil , Dieta , Fenóis/análise , Fenóis/farmacologia , Picratos/metabolismo , Extratos Vegetais/química , Plantas Comestíveis/química
5.
Acta Sci Pol Technol Aliment ; 20(3): 277-290, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34304546

RESUMO

BACKGROUND: The globe artichoke (Cynara scolymus L.) is a rich source of phenolic compounds which may be extracted by ultrasound technology and used as a medicinal alternative. The objective of this work was to determine the radiation amplitude (%), ethanol concentration (%), and time extraction (min) required to guarantee an elevated content of polyphenol compounds. METHODS: The optimal extraction conditions were assessed through the Box-Wilson design and by applying Composite Face Centered (CCFC) and total phenolic compounds (TPC) as the response variables. RESULTS: A quadratic model was adequate, with R2 = 0.993. The optimal conditions were a radiation amplitude of 97%, an ethanol concentration of 53%, and an extraction time of 9.7 min. The optimized extract of artichoke bracts (Cynara scolymus L.) showed a TPC of 25.13 (±0.030) mg GAE/g, an antioxidant activity DPPH of 39.79 (±0.014) mmol Trolox equivalents (TE), and an antioxidant capacity TEAC of 33.98 (±0.03) mmol Trolox equivalents. CONCLUSIONS: The results showed values closely related to the expected values, indicating that the models were well-developed.


Assuntos
Antioxidantes/análise , Cynara scolymus/química , Extratos Vegetais/química , Polifenóis/análise , Tecnologia Farmacêutica/métodos , Antioxidantes/farmacologia , Compostos de Bifenilo/metabolismo , Composição de Medicamentos , Etanol , Modelos Biológicos , Fenóis/análise , Fenóis/farmacologia , Fitoterapia , Picratos/metabolismo , Extratos Vegetais/farmacologia , Folhas de Planta/química , Polifenóis/farmacologia
6.
Int J Biol Macromol ; 183: 927-935, 2021 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-33971232

RESUMO

Human serum albumin (HSA) is widely used for the treatment of diverse clinical conditions to restore plasma volume, manage burns and treat hypoproteinemia.Although the HSA preparations should ideally preserve its functionality, the structural integrity and antioxidant properties of HSA may be compromised as a result of the manufacturing process. The present study examined seven commercially available HSA preparations for clinical use to investigate their post-translational modifications (PTMs) and antioxidant activity, including DPPH radical-scavenging, peroxyl radical antioxidant and metal binding activities, by means of mass spectrometry and Ellman's assay. The results confirmed that most of the PTMs of HSA and especially the oxidation of the free thiol residue varied between the different commercial albumins and the percentage of these PTMs were higher than those of physiological HSA. Moreover, HSA-DA isoform was increased at the end of the stability time and new oxidative modifications occurred in these samples. In conclusion, the bioprocesses for production of commercial albumins are responsible of their wide heterogeneity, being the ethanol fractionation and their storage conditions the more critical phases. Nonetheless, the Kedrion albumin shows a high content of free thiol and a lower concentration of PTMs than other commercial albumins.


Assuntos
Antioxidantes/metabolismo , Albumina Sérica Humana/metabolismo , Compostos de Bifenilo/metabolismo , Humanos , Oxirredução , Peróxidos/metabolismo , Picratos/metabolismo , Processamento de Proteína Pós-Traducional
7.
Fish Physiol Biochem ; 47(4): 1073-1085, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34021418

RESUMO

In this study, substance P, an antioxidant peptide of tachykinin, was identified using bioinformatics tools from the earlier established muscle transcriptome of a freshwater murrel Channa striatus and the peptide was named RM12. The antioxidant properties of RM12 were screened using various colorimetric assays. The toxicity of RM12 was experimented using fish erythrocytes, and it is observed that the maximum concentration (320 µM) of RM12 was found to have 15 or 20% of hemolytic activity; however, it was not significant with other tested concentrations (10, 20, 40, 80, and 160 µM). Further, the in vivo antioxidant properties of RM12 were experimented on zebrafish embryo, the intracellular ROS level was estimated by 5 mM H2O2 stress in the zebrafish embryo, and inhibition of apoptosis was evaluated. The antioxidant enzymes were extracted from the H2O2-stressed zebrafish embryo, and the intracellular ROS was eliminated due to RM12. Collectively, the experiment showed that the substance P from the freshwater murrel C. striatus possessed potent antioxidant properties; thus, it can further be focused to develop it as antioxidant molecule in aquaculture organisms.


Assuntos
Antioxidantes/farmacologia , Eritrócitos/efeitos dos fármacos , Peixes/metabolismo , Substância P/farmacologia , Animais , Compostos de Bifenilo/metabolismo , Catalase/metabolismo , Embrião não Mamífero/metabolismo , Eritrócitos/metabolismo , Feminino , Peixes/embriologia , Água Doce , Hemólise/efeitos dos fármacos , Radical Hidroxila/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Picratos/metabolismo , Superóxido Dismutase/metabolismo , Superóxidos/metabolismo
8.
Acta Sci Pol Technol Aliment ; 20(2): 179-188, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33884855

RESUMO

BACKGROUND: The aim of the study was to evaluate the caffeine level and antioxidant activity of brews of specialty grade coffee compared to popular coffee brands. METHODS: Ten types of coffee were used, including 7 specialty Arabica, 1 Robusta and 2 popular cheap coffee brands. For caffeine determination, HPLC analysis and the spectrophotometric method were used as reference. The total polyphenol content and antioxidant capacity (DPPH and FRAP methods) were evaluated. For two selected high-quality coffees, the influence of the brewing method on the antioxidant activity and caffeine content in the brews was assayed. RESULTS: Regarding the caffeine content, differences between specialty coffee brews and popular products were not found, and an average level amounted to 56 and 40 mg/ml, respectively. In contrast, the antioxidant capacity of specialty coffee brews was significantly higher than for popular ones, independently of the test used. The highest scavenging ability and total phenolic content was found for S3 specialty coffee (46.15% of DPPH inhibition and 58.7 mg GAE/ml, respectively), whereas the lowest was found for popular coffee (about 35% of DPPH inhibition and about 41 mg/GAE/ml). For two selected high-quality coffees, the influence of the brewing method on the antioxidant activity and caffeine content in the brews was tested. It was found that the use of a dripper (overflow brewing method) provides the brew with the best antioxidant properties but with moderate caffeine levels. CONCLUSIONS: It was found that 'specialty' quality coffees do not differ from popular brands in terms of caffeine content, but significantly outweigh them in terms of antioxidant activity. This property can be beneficial in the case of a high consumption of coffee, due to antiradical protective effects without the risk of caffeine overdose.


Assuntos
Antioxidantes/farmacologia , Cafeína/análise , Café/química , Manipulação de Alimentos/métodos , Polifenóis/farmacologia , Antioxidantes/análise , Compostos de Bifenilo/metabolismo , Coffea/química , Café/classificação , Humanos , Picratos/metabolismo , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Polifenóis/análise
9.
Acta Sci Pol Technol Aliment ; 20(2): 223-236, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33884859

RESUMO

BACKGROUND: Economically important vegetables are a strong source of antioxidants with different characteristics. Capsicum L. (pepper) is an important agricultural plant because of its economical, medicinal, and nutritional values. METHODS: This study aimed to test antioxidant parameters in the fruits of 9 cultivars of Capsicum annuum L. (CA 01-09), 7 cultivars of C. baccatum L. (CB 01-07), and 11 cultivars of C. chinense Jacq. (CC 01-11). The antioxidant activity of the investigated Capsicum cultivars was measured, along with the free radical scavenging activity (FRSA), using the DPPH method, and the molybdenum reducing power (MRP) was expressed as mg TE (Trolox equivalent) per g of DW (dry weight). Total polyphenol content (TPC), expressed as mg GAE (gallic acid equivalent) per g of DW, total flavonoid content (TFC), expressed as mg QE (quercetin equivalent) per g of DW, and total phenolic acid content (TPAC), expressed as mg CAE (caffeic acid equivalent) per g of DW, were the basic antioxidant parameters of antioxidant activity in this study. RESULTS: All investigated Capsicum extracts exhibited FRSA from 1.45 (CC-06) to 8.21 (CC-05) mg TE/g and MRP from 24.84 (CA-06) to 198.21 (CB-07) mg TE/g. The TPC of the tested extracts ranged from 10.13 (CB-03) to 38.68 (CB-07) mg GAE/g. The TFC of the studied samples showed values from 5.73 (CB-03) to 27.32 (CB-07) mg QE/g and TPAC from 2.24 (CB-03) to 13.07 (CC-07) mg CAE/g. A very strong correlation was found in the investigated cultivars between TPC and TPAC (r = 0.932, 0.839 and 0.848, respectively), and between TPC and TFC (r = 0.921, 0.982 and 0.939, respectively). Very strong relations were also found between TPC and FRSA (r = 0.820) in the C. annuum cultivars and between TPC and MRP (r = 0.898) in the C. baccatum cultivars. CONCLUSIONS: This study found useful results concerning the antioxidant potential of the fruits of Capsicum cultivars. The data obtained demonstrate the strong antioxidant activity of cultivars of Capsicum, which can be used in the food industry because of the commercial importance of these fruits.


Assuntos
Antioxidantes/farmacologia , Capsicum/química , Flavonoides/farmacologia , Fenóis/farmacologia , Extratos Vegetais/farmacologia , Antioxidantes/análise , Compostos de Bifenilo/metabolismo , Capsicum/classificação , Flavonoides/análise , Frutas/química , Humanos , Hidroxibenzoatos/análise , Hidroxibenzoatos/farmacologia , Fenóis/análise , Picratos/metabolismo , Extratos Vegetais/química , Polifenóis/análise , Polifenóis/farmacologia , Especificidade da Espécie , Verduras/química
10.
Acta Sci Pol Technol Aliment ; 20(2): 237-245, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33884860

RESUMO

BACKGROUND: In recent years, increasing health awareness in consumers has motivated breweries to expand their beverage ranges with products with increased biological value. The aim of the present research was to develop probiotic wort-based beverages with grapefruit or tangerine zest essential oil addition. METHODS: Wort was produced with 60% Pilsen malt, 20% Vienna malt and 20% Caramel Munich ІІ malt with and without the addition of 0.05% (v/v) grapefruit or tangerine essential oils. It was inoculated with the probiotic yeast strain Saccharomyces cerevisiae var. boulardii Y1. Fermentations were carried out at a constant temperature of 10°C for 5 days. The dynamics of the extract, the alcohol content and the concentration of viable cells were monitored daily. The total phenolic content, phenolic acid and flavonoid phenolic compounds were determined because of their antioxidant activity. The antioxidant activity was determined by radical scavenging assay (DPPH) and ferric reducing antioxidant power (FRAP). A descriptive organoleptic evaluation of the final beverages was performed. RESULTS: The essential oils inhibited yeast growth to some extent at the beginning of the fermentation, even at a concentration of 0.05% (v/v), which resulted in lower alcohol content in the beverages with essential oil addition. Nevertheless, at the end of fermentation the concentration of viable cells was almost equal in all the beverages. Tangerine essential oil addition led to the highest content of phenolics, of which phenolic acids predominated. Therefore, the highest antioxidant activity of the beverage with tangerine essential oil can be ascribed to phenolic acids. The results of the sensorial evaluation also showed that the panel had preference towards the beverage with tangerine essential oil. CONCLUSIONS: The combination of essential oil and the probiotic yeast strain resulted in beverages with higher biological value than the beverages produced with the probiotic strain alone. The results obtained will be used for optimisation of process variables in the production of pilot-scale wort-based probiotic beverages with essential oil addition.


Assuntos
Antioxidantes/farmacologia , Bebidas/microbiologia , Citrus/química , Fermentação , Manipulação de Alimentos/métodos , Óleos Voláteis/farmacologia , Probióticos , Antioxidantes/análise , Bebidas/análise , Compostos de Bifenilo/metabolismo , Citrus paradisi/química , Flavonoides/análise , Flavonoides/farmacologia , Microbiologia de Alimentos , Sucos de Frutas e Vegetais/análise , Sucos de Frutas e Vegetais/microbiologia , Humanos , Óleos Voláteis/química , Fenóis/análise , Fenóis/farmacologia , Picratos/metabolismo , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Saccharomyces cerevisiae
11.
Biotechnol Lett ; 43(1): 307-316, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32944816

RESUMO

OBJECTIVES: To assess the extracellular synthesis of silver nanoparticles using marine derived fungi Aspergillus brunneoviolaceus with their antibacterial and antioxidant activities. RESULTS: The biosynthesis of silver nanoparticles was estimated by the change in color from light yellow to dark brown within 36 h as the reaction progressed. UV-Visible spectroscopy exhibited its stability at 411 nm; ATR-FTIR spectroscopy depicted the functional group responsible for its production; X-Ray Diffraction denoted its crystalline FCC structure resembling the peaks in XRD pattern, corresponding to [111], [200], [220], [311] and [222] planes; TEM imaging revealed its spherical morphology with the particle size ranging from 0.72 to 15.21 nm and Tauc's plot analysis that disclosed its band gap energy as 2.44 eV that manifested the potential of AgNPs to be semiconductors. The characterization data henceforth, confirmed the efficient production of silver nanoparticles. The biosynthesized AgNPs expressed strong antibacterial activity against two Gram-positive and three Gram-negative bacteria. They also proved to possess higher antioxidative potentials by showing their potent radical scavenging activity against DPPH (2, 2-diphenyl-1-picrylhydrazyl). CONCLUSIONS: The study unfolds the prospect for further utilization of this mycogenically synthesized AgNPs as antibacterial, antioxidative and anticancer agents.


Assuntos
Antibacterianos , Antioxidantes , Aspergillus/química , Nanopartículas Metálicas/química , Prata , Antibacterianos/química , Antibacterianos/metabolismo , Antibacterianos/farmacologia , Antioxidantes/química , Antioxidantes/metabolismo , Antioxidantes/farmacologia , Organismos Aquáticos/química , Bactérias/efeitos dos fármacos , Produtos Biológicos/química , Compostos de Bifenilo/metabolismo , Picratos/metabolismo , Prata/química , Prata/metabolismo
12.
Protein Expr Purif ; 177: 105748, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-32911063

RESUMO

The antioxidant activity and cell viability of feather hydrolysates obtained with the Bacillus licheniformis were evaluated using an in-vitro model. The results indicate that feathers-derived peptides under 3 kDa have antioxidant activity with IC50 values of 5.03 ± 0.215 mg/mL by using DPPH antioxidant assay. Although the antioxidant activity of the peptides under 3 kDa preserved after applying diverse heating (from 20 to 100 °C), they lost their activity under strongly acidic or alkaline conditions. Antioxidant activity of the mixed feather bioactive peptides (MFBPs) obtained with partial purification of peptides under 3 kDa was with IC50 amount of 0.169 mg/mL ± 0.004 using DPPH radical scavenging assay. Also, MFBPs within an amount range of from 0.0048 to 5.0 mg/mL, illustrated no cytotoxicity to gingival fibroblast blood cell lines. In light of our results, the obtained value-added peptides could be useful in different food products as a future functional ingredient with antioxidant potency.


Assuntos
Antioxidantes/farmacologia , Bacillus licheniformis/química , Plumas/química , Queratinas/farmacologia , Peptídeos/farmacologia , Animais , Antioxidantes/química , Antioxidantes/isolamento & purificação , Bacillus licheniformis/enzimologia , Compostos de Bifenilo/antagonistas & inibidores , Compostos de Bifenilo/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Galinhas , Temperatura Alta , Humanos , Hidrólise , Queratinas/química , Queratinas/isolamento & purificação , Peso Molecular , Neuroglia/efeitos dos fármacos , Neuroglia/metabolismo , Neuroglia/patologia , Peptídeos/química , Peptídeos/isolamento & purificação , Picratos/antagonistas & inibidores , Picratos/metabolismo
13.
Acta Sci Pol Technol Aliment ; 19(3): 261-270, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32978909

RESUMO

BACKGROUND: The search for new sources of natural antioxidants is very important because many diseases are caused by oxidative stress. Fruit which contain antioxidants are an important part of a healthy diet. The aim of this study was to evaluate the antioxidant activity of extracts of both the fresh and frozen peel and the flesh of Garcinia mangostana L. METHODS: The extracts from the fresh and frozen peel and the flesh of mangosteen were prepared by ultrasound-assisted extraction using 20%, 40%, 70% and 96% (v/v) ethanol for 15, 30 or 60 minutes. The antioxidant potential was evaluated by the DPPH, ABTS, CUPRAC, FRAP and FIC methods, whereas the total phenolic content was measured using the Folin-Ciocalteu (F-C) technique. The contents of anthocyanins and flavonoids in the peel extracts were also determined. RESULTS: In most cases, the highest antioxidant activity was observed in the fresh peel samples. It was higher than the antioxidant potential of the frozen peels and the fresh and frozen flesh. The ultrasound-assisted extraction, in particular those lasting 30 or 60 minutes and using ethanol in concentrations higher than 20% (v/v), seemed to be an effective extraction process. CONCLUSIONS: The obtained results suggest that G. mangostana, in particular its peels, could be a valuable source of antioxidants. The extraction parameters, such as the time or solvent concentration, as well as the type of plant material, had an impact on the tested properties of the extracts. However, more detailed studies on the antioxidant activity of the studied plants are required.


Assuntos
Antioxidantes/farmacologia , Frutas/química , Garcinia mangostana/química , Extratos Vegetais/farmacologia , Polifenóis/farmacologia , Antocianinas/análise , Antocianinas/farmacologia , Antioxidantes/análise , Benzotiazóis/metabolismo , Compostos de Bifenilo/metabolismo , Flavonoides/análise , Flavonoides/farmacologia , Conservação de Alimentos/métodos , Armazenamento de Alimentos/métodos , Congelamento , Humanos , Fenóis/análise , Fenóis/farmacologia , Picratos/metabolismo , Epiderme Vegetal/química , Extratos Vegetais/química , Polifenóis/análise , Ácidos Sulfônicos/metabolismo
14.
Molecules ; 25(13)2020 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-32640504

RESUMO

Plants and plant-based products have been used for a long time for medicinal purposes. This study aimed to determine the antioxidant and anti-α-glucosidase activities of eight selected underutilized plants in Malaysia: Leucaena leucocephala, Muntingia calabura, Spondias dulcis, Annona squamosa, Ardisia elliptica, Cynometra cauliflora, Ficus auriculata, and Averrhoa bilimbi. This study showed that the 70% ethanolic extract of all plants exhibited total phenolic content (TPC) ranging from 51 to 344 mg gallic acid equivalent (GAE)/g dry weight. A. elliptica showed strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO) scavenging activities, with half maximal inhibitory concentration (IC50) values of 2.17 and 49.43 µg/mL, respectively. Most of the tested plant extracts showed higher inhibition of α-glucosidase enzyme activity than the standard, quercetin, particularly A. elliptica, F. auriculata, and M. calabura extracts with IC50 values of 0.29, 0.36, and 0.51 µg/mL, respectively. A total of 62 metabolites including flavonoids, triterpenoids, benzoquinones, and fatty acids were tentatively identified in the most active plant, i.e., A. elliptica leaf extract, by using ultra-high-performance liquid chromatography (UHPLC)-electrospray ionization (ESI) Orbitrap MS. This study suggests a potential natural source of antioxidant and α-glucosidase inhibitors from A. elliptica.


Assuntos
Ardisia/química , Inibidores de Glicosídeo Hidrolases/análise , Fenóis/análise , Extratos Vegetais/química , Plantas Medicinais/química , Antioxidantes/química , Ardisia/enzimologia , Benzoquinonas/química , Compostos de Bifenilo/metabolismo , Cromatografia Líquida de Alta Pressão , Fabaceae/química , Fabaceae/enzimologia , Ácidos Graxos/análise , Flavonoides/análise , Inibidores de Glicosídeo Hidrolases/química , Concentração Inibidora 50 , Malásia , Espectrometria de Massas , Óxido Nítrico/metabolismo , Picratos/metabolismo , Extratos Vegetais/análise , Caramujos/química , Triterpenos/análise
15.
Acta Sci Pol Technol Aliment ; 19(2): 169-176, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32600013

RESUMO

BACKGROUND: The chemical synthesis of antioxidants has exposed more and more of their disadvantages. Therefore, the development of safe, healthy and efficient natural antioxidants has become a research hotspot. At present, antioxidant peptides are gradually becoming more popular due to their strong antioxidant activity and high safety. METHODS: The aim of this experiment was to obtain the optimum fermentation conditions for producing antioxidant peptides from Lactobacillus casei L61. The effects of various factors (fermentation temperature, fermentation time, concentration of regenerated milk and inoculum) on the production of antioxidant peptides by Lactobacillus casei L61 were studied using a single factor test. Four methods (DPPH radical scavenging activity, the ability to chelate iron, hydroxyl radical scavenging rate, total reduction force) were used to determine antioxidant activity in vitro. The optimum fermentation conditions were determined by orthogonal experiment. RESULTS: The results showed that the optimal fermentation conditions for the production of antioxidant peptides by L61 were 11% reconstituted milk, 5% inoculated milk, fermentation at 41℃ and 16 hours of fermentation time. Under these conditions, the results of the orthogonal test showed that the DPPH radical scavenging rate of the whey sample was 59.97 ±0.87%, which was higher than those of the other 9 groups, and significantly higher than that of the control group (41.97 ±1.37%). CONCLUSIONS: The concentration of reconstituted milk and the amount of inoculated milk had a significant effect on the production of antioxidant peptides by Lactobacillus casei L61. It provides a reference for the optimization of the fermentation nutritional composition of antioxidant peptides produced by Lactobacillus casei L61.


Assuntos
Antioxidantes , Cabras , Lactobacillus casei/metabolismo , Peptídeos , Soro do Leite , Animais , Antioxidantes/análise , Antioxidantes/farmacologia , Compostos de Bifenilo/metabolismo , Produtos Fermentados do Leite , Fermentação , Humanos , Leite/microbiologia , Peptídeos/análise , Peptídeos/farmacologia , Picratos/metabolismo , Soro do Leite/metabolismo , Soro do Leite/microbiologia , Proteínas do Soro do Leite/metabolismo
16.
Acta Sci Pol Technol Aliment ; 19(2): 195-205, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32600016

RESUMO

BACKGROUND: Sea buckthorn is a good and possible source of a wide range of bioactive compounds with     a positive effect on the human body, especially polyphenols and carotenoids. METHODS: The present study aimed to evaluate the antioxidant and antimicrobial activities of Hippophae rhamnoides L. products - 100% oil, 100% juice, dry berries, and tea (dry berries, leaves, and twigs). The antioxidant activity was determined by DPPH radical scavenging and molybdenum reducing antioxidant power methods. The content of phytochemicals (polyphenols, flavonoids, carotenoids) was measured by colorimetric methods. The detection of antimicrobial activity was carried out using the disc diffusion method and an evaluation of minimal inhibition concentration against three species of Gram-negative bacteria: Escherichia coli CCM 3988, Salmonella enterica subsp. enterica CCM 3807, Yersinia enterocolitica CCM 5671, and three Gram-positive bacteria: Bacillus thuringiensis CCM 19, Listeria monocytogenes CCM 4699, Staphylococcus aureus subsp. aureus CCM 2461. RESULTS: All the tested sea buckthorn products had a high antioxidant activity. The highest content of total polyphenols (204.26 mg GAE/g), flavonoids (30.00 mg QE/g), and carotenoids (0.34 mg/g) were identified in 100% sea buckthorn juice. Maximal values of the antioxidant activity were found using the DPPH method for 100% oil (8.75 mg TE/g) and molybdenum reducing power for tea (196.41 mg TE/g). All the tested products showed strong antimicrobial activity against the tested bacteria, confirmed by both methods - disc diffusion (especially for Yersinia enterocolitica CCM 5671) and minimal inhibitory concentration. CONCLUSIONS: The pronounced antioxidant and antibacterial properties of sea buckthorn products indicate the importance of sea buckthorn application in health promotion and agricultural practice.


Assuntos
Antibacterianos/farmacologia , Antioxidantes/farmacologia , Carotenoides/farmacologia , Flavonoides/farmacologia , Hippophae/química , Extratos Vegetais/farmacologia , Polifenóis/farmacologia , Antibacterianos/análise , Antioxidantes/análise , Bactérias/efeitos dos fármacos , Compostos de Bifenilo/metabolismo , Carotenoides/análise , Cromatografia Líquida de Alta Pressão , Flavonoides/análise , Humanos , Testes de Sensibilidade Microbiana , Molibdênio/metabolismo , Fenóis/análise , Fenóis/farmacologia , Compostos Fitoquímicos/análise , Compostos Fitoquímicos/farmacologia , Picratos/metabolismo , Componentes Aéreos da Planta/química , Extratos Vegetais/química , Óleos Vegetais/química , Polifenóis/análise
17.
Biomed Res Int ; 2020: 2096947, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32566667

RESUMO

The objective of this study was to authenticate the ethnobotanical claims of the Nepeta ruderalis Buch.-Ham. (N. ruderalis) extract in the traditional system of medicine. Crude extract was prepared via a simple maceration process. DPPH free radical scavenging and carrageenan-induced rat paw edema models were used to monitor antioxidant and anti-inflammatory responses of the N. ruderalis extract. Furthermore, it was tested for antiplatelet aggregation, cardioprotective, and calcium channel antagonistic activities via standard documented protocols. The N. ruderalis extract exhibited 80.82% antioxidant activity (IC50 = 207.51 ± 4.36 µg) while the anti-inflammatory response was significant (p < 0.05 to p < 0.01) at 50 mg/kg (45.58%) and 100 mg/kg (60.90%) doses. Moreover, it was found to inhibit platelet aggregation (IC50 = 1.06 and 0.91 mg/mL) and, in addition, to increase the force of contraction at the concentration of 3.0-10 mg/mL with a decrease in the heart rate on isolated paired atria (EC50 = 11.78 mg/mL). Relaxant activity was observed on the isolated rabbit jejunum (EC50 = 0.96 mg/mL) and trachea (EC50 = 0.89 mg/mL). However, in a cumulative way, an 80-millimolar potassium-induced contraction was evaluated (EC50 = 1.31 mg/mL). The N. ruderalis extract exhibited antioxidant, anti-inflammatory, platelet aggregating, cardiotonic, and calcium channel antagonistic activities, therefore proving scientifically its effectiveness in the traditional system of medicine.


Assuntos
Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Fármacos Cardiovasculares/farmacologia , Nepeta/química , Extratos Vegetais/farmacologia , Animais , Compostos de Bifenilo/metabolismo , Edema/patologia , Átrios do Coração/efeitos dos fármacos , Frequência Cardíaca/efeitos dos fármacos , Jejuno/efeitos dos fármacos , Picratos/metabolismo , Inibidores da Agregação Plaquetária/farmacologia , Coelhos , Ratos , Traqueia/efeitos dos fármacos
18.
Molecules ; 25(11)2020 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-32521731

RESUMO

Actinopyga lecanora (A. lecanora) is classified among the edible species of sea cucumber, known to be rich in protein. Its hydrolysates were reported to contain relatively high antioxidant activity. Antioxidants are one of the essential properties in cosmeceutical products especially to alleviate skin aging. In the present study, pH, reaction temperature, reaction time and enzyme/substrate ratio (E/S) have been identified as the parameters in the papain enzymatic hydrolysis of A. lecanora. The degree of hydrolysis (DH) with antioxidant activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric-reducing antioxidant power (FRAP) assays were used as the responses in the optimization. Analysis of variance (ANOVA), normal plot of residuals and 3D contour plots were evaluated to study the effects and interactions between parameters. The best conditions selected from the optimization were at pH 5.00, 70 °C of reaction temperature, 9 h of hydrolysis time and 1.00% enzyme/substrate (E/S) ratio, with the hydrolysates having 51.90% of DH, 42.70% of DPPH activity and 109.90 Fe2+µg/mL of FRAP activity. The A. lecanora hydrolysates (ALH) showed a high amount of hydrophobic amino acids (286.40 mg/g sample) that might be responsible for antioxidant and antityrosinase activities. Scanning electron microscopy (SEM) image of ALH shows smooth structures with pores. Antityrosinase activity of ALH exhibited inhibition of 31.50% for L-tyrosine substrate and 25.40% for L-DOPA substrate. This condition suggests that the optimized ALH acquired has the potential to be used as a bioactive ingredient for cosmeceutical applications.


Assuntos
Antioxidantes/farmacologia , Compostos de Bifenilo/metabolismo , Inibidores Enzimáticos/farmacologia , Compostos Férricos/química , Monofenol Mono-Oxigenase/antagonistas & inibidores , Picratos/metabolismo , Hidrolisados de Proteína/farmacologia , Pepinos-do-Mar/química , Animais , Oxirredução , Papaína/metabolismo
19.
Iran Biomed J ; 24(5): 314-23, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32429645

RESUMO

Background: The Peucedanum species have many pharmacological effects due to the presence of coumarins, flavonoids, phenolic compounds, and essential fatty acids in these species. In this study, for the first time, the anticancer activity of Peucedanum chenur methanolic extract via the induction of apoptosis and inhibition of invasion in HCT-116 human colon cancer cells was investigated. Methods: P. chenur methanolic extract effect on HCT-116 cells viability and antioxidant activity were evaluated using MTT assay, 1,1-Diphenyl-2-picrylhydrazyl, and iron chelating tests, respectively. Changes in mRNA expression level in a panel of relevant genes were assessed by the quantitative real-time PCR. Also, apoptosis was assessed by cell cycle analysis and Annexin V/PI (propidium iodide) method, and the effect on cell migration was tested using scratch test. Results: P. chenur methanolic extract increased significantly the expression of BAX while decreased the expression of BCL-2, AKT1, FAK, RhoA, and matrix metalloproteinase (MMP) genes compared to the control group. BAX/BCL-2 ratio and apoptosis elevated, whereas cell migration reduced significantly. Besides, our extract showed an appropriate antioxidant activity. Conclusion: P. chenur may be introduced as a new chemopreventive agent in medicine due to its notable power in terms of induction of apoptosis and inhibition of invasion.


Assuntos
Antineoplásicos Fitogênicos/uso terapêutico , Apiaceae/química , Apoptose , Neoplasias Colorretais/tratamento farmacológico , Neoplasias Colorretais/patologia , Componentes Aéreos da Planta/química , Antineoplásicos Fitogênicos/farmacologia , Antioxidantes/metabolismo , Apoptose/efeitos dos fármacos , Compostos de Bifenilo/metabolismo , Linhagem Celular Tumoral , Ensaios de Migração Celular , Sobrevivência Celular/efeitos dos fármacos , Neoplasias Colorretais/genética , DNA de Neoplasias/metabolismo , Sequestradores de Radicais Livres/farmacologia , Fase G1/efeitos dos fármacos , Fase G1/genética , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Quelantes de Ferro/farmacologia , Metanol , Invasividade Neoplásica , Proteínas de Neoplasias/genética , Proteínas de Neoplasias/metabolismo , Picratos/metabolismo , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Extratos Vegetais/toxicidade
20.
Pharmacology ; 105(11-12): 645-651, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32289807

RESUMO

INTRODUCTION: Peptic lesions usually develop when there is an imbalance between aggressive drivers and gastro-protective mediators that guard the lining of the gastrointestinal tract. The most crucial of these mediators are antioxidants, whose loss may predispose to oxidative stress, which is believed to be the main aggravator of several diseases including peptic ulcer. Proton pump inhibitors (PPIs) are drugs that are highly effective and widely used for therapeutic management of peptic disorders through inhibition of gastric acid secretion. In spite of this, oxidative damage may continue to be a major issue that can predispose to future lesions. OBJECTIVE: The present study is designed to explore the possible antioxidant capability of different PPIs, including omeprazole, esomeprazole, lansoprazole, pantoprazole, and rabeprazole, in an aim to suggest an agent that, in addition to its acid-suppression properties, can provide antioxidant profit. METHODS: The antioxidant activity of different PPIs was evaluated calorimetrically to test the ability of each drug to quench oxygen free radical, using the well-known stable free radical α,α-diphenyl-ß-picrylhydrazyl (DPPH), and compared to ascorbic acid (AA; vitamin C). The measurements were performed using a spectrophotometer at 517 nm. RESULTS: All the studied drugs reduced DPPH, but to different extents. However, omeprazole and esomeprazole showed the highest ability to scavenge free radicals (50% inhibitory concentrations [IC50s] of the percentage for free radical scavenging activity are 18.7 ± 5.7 and 18.7 ± 5.7, respectively, and the AA equivalents are 83,772 ± 11,887 and 81,732 ± 8,523 mg AA/100 g, respectively). Conversely, lansoprazole, pantoprazole, and rabeprazole might be having no role in this story (IC50s of the percentage for free radical scavenging activity are 49.3 ± 3.1, 49 ± 9.4, and 40.7 ± 7.2, respectively, and the AA equivalents are 30,458 ± 3,884, 32,222 ± 10,377, and 37,876 ± 8,816 mg AA/100 g, respectively). CONCLUSION: Thus, omeprazole and esomeprazole may confer a significant dual action in gastrointestinal protection by providing potent antioxidant properties in addition to their major role as acid-suppression agents. However, further studies are essential to elucidate the mechanism behind the difference between the drugs of the same class.


Assuntos
Antioxidantes/farmacologia , Esomeprazol/farmacologia , Lansoprazol/farmacologia , Omeprazol/farmacologia , Pantoprazol/farmacologia , Inibidores da Bomba de Prótons/farmacologia , Rabeprazol/farmacologia , Ácido Ascórbico/farmacologia , Compostos de Bifenilo/metabolismo , Radicais Livres/metabolismo , Técnicas In Vitro , Concentração Inibidora 50 , Picratos/metabolismo , Espectrofotometria
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