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1.
Food Chem ; 335: 127655, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-32731125

RESUMO

In the present study, the profile of phenolic compounds in colored (white, yellow, black and blue) naked barley was detected and their content and antioxidant abilities were investigated. The results showed that there were 156 phenolic substances identified, including monophenol, phenolic acids, flavonoids and other polyphenols. The black sample had the most types of phenolic. The content of phenolic varies depending on color of naked barley and the highest values of total phenolic acid and total flavonoids were observed in black and white samples. Furthermore, the strongest ferric reducing antioxidant power and the free radical scavenging ability of DPPH, ABTS, and superoxide anion showed in white, white, yellow and black naked barley. While white and yellow samples had the strongest scavenging ability of hydroxyl radical. There was significant correlation between phenolic components and anti-oxidation. This study suggests that colored naked barley grains are rich in phenolic compounds with antioxidant capacity.


Assuntos
Antioxidantes/análise , Antioxidantes/química , Hordeum/química , Fenóis/análise , Sementes/química , Compostos de Bifenilo/química , Flavonoides/análise , Depuradores de Radicais Livres/química , Hidroxibenzoatos/análise , Radical Hidroxila/química , Oxirredução , Picratos/química , Pigmentação , Polifenóis/análise , Superóxidos/química
2.
Food Chem ; 339: 128110, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33152887

RESUMO

Piperine, as the most abundant alkaloid in pepper, gained a lot of attention for possible antioxidant and therapeutic properties. Electrochemical techniques were applied to widely evaluate the redox behavior of piperine by comparison to that of well-known antioxidants: ascorbic acid, protocatechuic acid, syringic acid, tyrosine and capsaicin used as controls. Also, electrochemistry was involved in an innovative way to investigate the potential antioxidant properties of piperine combined with different in vitro peroxidation and reducing assays: (i) 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging; (ii) 2,2,6,6-tetramethylpiperidinyl-1-oxy (TEMPO) scavenging; (iii) ferric ions (Fe3+) reducing power; (iv) hydrogen peroxide (H2O2) scavenging. Results show that piperine readily reacts with highly oxidizing radicals and bind redox-active metal ions in a similar manner as antioxidants used as model.


Assuntos
Alcaloides/química , Antioxidantes/química , Benzodioxóis/química , Piperidinas/química , Alcamidas Poli-Insaturadas/química , Ácido Ascórbico/química , Compostos de Bifenilo/química , Óxidos N-Cíclicos/química , Técnicas Eletroquímicas , Depuradores de Radicais Livres/química , Radicais Livres/metabolismo , Peróxido de Hidrogênio/química , Ferro/química , Oxirredução , Picratos/química
3.
PLoS One ; 15(9): e0239076, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32931505

RESUMO

Dandelion (Taraxacum officinale), a common plant worldwide, is used as both a medicine and food. Fermentation is a food processing technology that has many advantages, such as low energy cost, changes in product characteristics, and enhanced product quality. The purpose of this study was to investigate the effect of solid-state fermentation (SSF) on the content, composition and antioxidant activity of dandelion flavonoids. Response surface methodology was used to optimize dandelion fermentation conditions. Under optimized conditions, the maximum flavone concentration was 66.05 ± 1.89 mg/g. The flavonoid content of the crude extract from fermented dandelion (FDF) was 183.72 ± 2.24 mg/g. The flavonoid compounds in the crude extracts were further identified by UPLC-ESI-MS/MS. A total of 229 flavonoid compounds were identified, and 57 differential flavonoids (including 27 upregulated and 30 downregulated flavonoids) between FDF and the crude extract of unfermented dandelion (DF) were observed, of which 25 were annotated to metabolic pathways. FDF displayed superior antioxidant activity to that of DF in in vitro DPPH radical-scavenging and reducing power assays. The favorable results of our investigation could provide a new way for the exploitation and utilization of dandelion, which could be promising for its application as an antioxidant and functional food additive with flavonoids as ingredients.


Assuntos
Antioxidantes/análise , Flavonoides/análise , Taraxacum/química , Antioxidantes/farmacologia , Compostos de Bifenilo/química , Fermentação , Flavonoides/farmacologia , Radicais Livres/química , Picratos/química , Extratos Vegetais/análise , Extratos Vegetais/farmacologia , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas em Tandem
4.
PLoS One ; 15(8): e0237844, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32834013

RESUMO

The feasibility of the production of germinated rice extracts using indigenous rice from Southern Thailand, including Khemtong (KHT) and Khai Mod Rin (KMR) from a single location at the Pak Phanang River Basin in Nakhon Si Thammarat, was investigated. The nutritional composition and bioactivity of the germinated rice extracts from both cultivars were evaluated. Optimum germination time for both rices was 96 h, leading to the highest GABA, thiamine, free amino acid, total sugar, and α-amylase activity (p<0.05). Germinated KHT had a higher α-amylase activity than germinated KMR at all germination times. Mashing at 60°C/pH 5.5 rendered the extract with the highest GABA content (p<0.05) and desirable contents of other nutrients. In comparison with germinated Sungyod (local colored rice) and Jasmine (commercial Thai rice) extracts, KHT and KMR showed a higher scavenging activity against DPPH•, OH•, and H2O2 (p<0.05) with a comparable ABTS•+ inhibition. For metal chelation, reducing power and ACE inhibitory activity, the germinated Sungyod extract was greater than KHT/KMR. The results demonstrated the potential use of germinated local Thai rice for the production of functional beverages.


Assuntos
Germinação , Fenômenos Fisiológicos da Nutrição , Oryza/química , Oryza/crescimento & desenvolvimento , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Aminoácidos/análise , Inibidores da Enzima Conversora de Angiotensina/farmacologia , Antioxidantes/farmacologia , Compostos de Bifenilo/química , Estudos de Viabilidade , Flavonoides/análise , Depuradores de Radicais Livres/química , Minerais/análise , Fenóis/análise , Ácido Fítico/análise , Picratos/química , Açúcares/análise , Tiamina/análise , Fatores de Tempo , alfa-Amilases/metabolismo , Ácido gama-Aminobutírico/análise
5.
Int J Nanomedicine ; 15: 4021-4047, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32606662

RESUMO

Purpose: Periodontitis is a chronic inflammatory disease associated with microbial accumulation. The purpose of this study was to reuse the agricultural waste to produce cellulose nanofibers (CNF) and further modification of the CNF with κ-carrageenan oligosaccharides (CO) for drug delivery. In addition, this study is focused on the antimicrobial activity of surfactin-loaded CO-CNF towards periodontal pathogens. Materials and Methods: A chemo-mechanical method was used to extract the CNF and the modification was done by using CO. The studies were further proceeded by adding different quantities of surfactin [50 mg (50 SNPs), 100 mg (100 SNPs), 200 mg (200 SNPs)] into the carrier (CO-CNF). The obtained materials were characterized, and the antimicrobial activity of surfactin-loaded CO-CNF was evaluated. Results: The obtained average size of CNF and CO-CNF after ultrasonication was 263 nm and 330 nm, respectively. Microscopic studies suggested that the CNF has a short diameter with long length and CO became cross-linked to form as beads within the CNF network. The addition of CO improved the degradation temperature, crystallinity, and swelling property of CNF. The material has a controlled drug release, and the entrapment efficiency and loading capacity of the drug were 53.15 ± 2.36% and 36.72 ± 1.24%, respectively. It has antioxidant activity and inhibited the growth of periodontal pathogens such as Streptococcus mutans and Porphyromonas gingivalis by preventing the biofilm formation, reducing the metabolic activity, and promoting the oxidative stress. Conclusion: The study showed the successful extraction of CNF and modification with CO improved the physical parameters of the CNF. In addition, surfactin-loaded CO-CNF has potential antimicrobial activity against periodontal pathogens. The obtained biomaterial is economically valuable and has great potential for biomedical applications.


Assuntos
Carragenina/química , Celulose/química , Lipopeptídeos/química , Nanofibras/química , Peptídeos Cíclicos/química , Periodonto/microbiologia , Animais , Bactérias/metabolismo , Compostos de Bifenilo/química , Sobrevivência Celular , Difusão Dinâmica da Luz , Depuradores de Radicais Livres/química , Malondialdeído/metabolismo , Camundongos , Testes de Sensibilidade Microbiana , Nanofibras/ultraestrutura , Oligossacarídeos/química , Picratos/química , Células RAW 264.7 , Soja/química , Espectroscopia de Infravermelho com Transformada de Fourier
6.
J Chromatogr A ; 1623: 461150, 2020 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-32505270

RESUMO

Antioxidants play an essential role in human health, as they have been found to be capable of lowering the incidence of many diseases, such as cancer and angiocardiopathy. Currently, more attention is paid to natural antioxidants because of the possible insecurity of synthetic antioxidants. Thus, the development of efficient techniques or methods to separate antioxidants from natural sources is requested urgently. High-speed counter current chromatography (HSCCC) is a unique support-free liquid-liquid chromatographic technique and has been widely applied in the field of separation of natural products. In this review, we summarize and analyze the related researches on the application of HSCCC in the separation of various natural antioxidants so far. The purpose of the article is to provide a certain theoretical support for the separation of natural antioxidants by HSCCC, and to make full use of advantages of HSCCC in the separation of bioactive components. In particular, some key problems associated with the separation strategies, the structural categories of natural antioxidants, solvent system choices, and the application of different elution modes in HSCCC separation, are summarized and commented. We expect that the content reviewed can offer more evidence for the development of the field of natural antioxidants separation, so as to achieve large-scale preparation of natural antioxidants.


Assuntos
Antioxidantes/isolamento & purificação , Distribuição Contracorrente/métodos , Compostos de Bifenilo/química , Cromatografia Líquida de Alta Pressão , Humanos , Concentração de Íons de Hidrogênio , Picratos/química , Solventes/química
7.
Carbohydr Polym ; 237: 116158, 2020 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-32241435

RESUMO

Starch rich foods are almost indispensable in mundane diet of people round the globe. Rapid digestibility of starch culminates into elevated blood glucose level which is an evident factor for many diseases. To curb this rapid digestibility and the elevated glycemic response, resistant starch content in highly nutritious but unexplored popped makhana (Euryale ferox) was increased by amylopullulanase treatment. In the present study, amylopullulanase treated makhana flour (MM) was compared with the native makhana flour (NM) based on physicochemical and functional properties, where enhanced amylose content, resistant starch and crystallinity were recorded to be 12.33 %, 14.88 % and 11.32 % respectively, whereas, readily digestible starch and oil holding capacity decreased by 13.01 % and 3.12 g/g respectively. The present study ensures the reduction and sustainable release of glucose during in vitro digestibility analysis. These findings point out the elevated potential of amylopullulanase treated makhana flour for therapeutic food formulation.


Assuntos
Farinha , Glicosídeo Hidrolases/química , Nozes , Nymphaeaceae , Amido/química , Amilases/química , Antioxidantes/química , Compostos de Bifenilo/química , Alimento Funcional , Glucana 1,4-alfa-Glucosidase/química , Picratos/química
8.
Chemosphere ; 253: 126728, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32298913

RESUMO

Nile perch wastewater was biodegraded using two Bacillus species to recover bioactive substances to enhance its reutilization value. The two Bacillus species successfully produced low-molecular-weight substances with a 47.8% degree of hydrolysis. The antioxidant activities of the Nile perch wastewater increased as the biodegradation proceeded, and the culture supernatant exhibited the highest DPPH (80.1%), ABTS (93.1%) and Fe2+ chelating (88.5%) antioxidant activities at 60 h. The antioxidant potential of the biodegraded Nile perch wastewater was found to be higher than those of other fish hydrolysates. Moreover, the biodegraded Nile perch wastewater exhibited effective antimicrobial activity against Vibrio vulnificus, exhibiting a minimal inhibitory concentration of 585 µg mL-1. Two-dimensional thin layer chromatography analysis revealed the specific amino acids responsible for the antioxidant activity, and molecular-weight cut-off ultrafiltration revealed that the <2-kDa fraction exhibited the highest antioxidant activity with the lowest IC50 values (0.43 and 0.22 mg mL-1 for DPPH and ABTS antioxidant activities, respectively). This is the first report of the reutilization of Nile perch wastewater as a natural antioxidant and antimicrobial ingredient for nutraceuticals.


Assuntos
Antibacterianos/isolamento & purificação , Antioxidantes/isolamento & purificação , Bacillus/metabolismo , Peptídeos/isolamento & purificação , Percas , Águas Residuárias/química , Aminoácidos/isolamento & purificação , Aminoácidos/farmacologia , Animais , Antibacterianos/farmacologia , Antioxidantes/farmacologia , Biodegradação Ambiental , Compostos de Bifenilo/química , Quelantes/isolamento & purificação , Quelantes/farmacologia , Pesqueiros , Hidrólise , Peso Molecular , Peptídeos/farmacologia , Percas/crescimento & desenvolvimento , Picratos/química , Ultrafiltração , Vibrio vulnificus/efeitos dos fármacos
9.
Sci Rep ; 10(1): 3847, 2020 03 02.
Artigo em Inglês | MEDLINE | ID: mdl-32123269

RESUMO

The effects of different concentrations (0, 50,100, 1000 and 2500 mg/L) of engineered aluminum and nickel oxide nanoparticles (Al2O3 and NiO NPs) on plant growth, oxidative stress and antioxidant activities in the hydroponically grown tissues of Nigella arvensis L. were investigated. The plant biomass was significantly increased under 50 and 100 mg/L of Al2O3 NPs or 50 mg/L of NiO NPs treatment, but was significantly decreased at higher concentrations of these nanoparticles. Assays of several enzymatic antioxidants such as ascorbate peroxidase (APX), catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD) in roots and shoots indicate a general increase of activities after exposure to 50-2,500 mg/L of Al2O3 NPs and NiO NPs. The results are corroborated by an increased 2,2-diphenyl-1-picryl hydrazyl (DPPH) scavenging activity, total antioxidant capacity, total reducing power, total iridoids content, total saponin content, and total phenolic content in treated plants by Al2O3 NPs compared to the control plants. By contrast, the antioxidant activities, formation of secondary metabolites, and other related physiological parameters such as the total antioxidant capacity, DPPH scavenging activity and total saponin content were inhibited after the concentration of NiO NPs was increased to 100 mg/L. Total phenols, saponins, iridoids and total antioxidant content and DPPH scavenging activity were increased in plants treated with 100-2,500 mg/L Al2O3 NPs. Overall, these two nanoparticles displayed different effects in the shoots and roots of plants at different concentrations, which may be due to their physico-chemical properties.


Assuntos
Alumínio/química , Alumínio/farmacologia , Antioxidantes/metabolismo , Nanopartículas Metálicas/química , Níquel/química , Níquel/farmacologia , Nigella/efeitos dos fármacos , Compostos de Bifenilo/química , Engenharia , Peróxido de Hidrogênio/metabolismo , Nigella/crescimento & desenvolvimento , Nigella/metabolismo , Picratos/química
10.
J Photochem Photobiol B ; 205: 111842, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32146274

RESUMO

Development of high responsive photocatalysts for the degradation of dye from water is a significance method to solve the difficulties of water contamination. In the present project, Cr2O3 nanoparticles were decorated onto cellulose through a facile synthesis method, which was exposed to characterization by XRD, FESEM, DLS, PL, and UV-vis spectroscopy. The structural attributes confirmed the presence of rhombohedral phase of Cr2O3 nanoparticles. The mean crystal size of Cr2O3, and Cr2O3/cellulose nanocomposites were 38.50 nm, and 50.11 nm, respectively. The band gap values (Eg) of Cr2O3, and Cr2O3/cellulose nanocomposites were was found 3.00, and 2.53 eV, respectively. Moreover, the morphological and optical studies have been showed the impressive photocatalytic properties of the prepared Cr2O3/cellulose nanocomposites. The photocatalytic efficiency of Cr2O3, and Cr2O3/cellulose nanocomposites has been investigated for the photo-degradation of crystal violet in the ultraviolet light region. The Cr2O3/cellulose indicated promising photocatalytic performance and up to 99.65% of the crystal violet was photo-degraded in 40 min. The obtained crystal violet degradation results were fitted onto a Langmuir-Hinshelwood (L-H) plot. The antioxidant performances of Cr2O3, and Cr2O3/cellulose were analyzed. The beneficial antibacterial performance of the Cr2O3/cellulose nanocomposites was tested by various bacteria as Escherichia coli, Pseudomonas aeruginosa, staphylococcus aurous, and Streptococcus pyogenes.


Assuntos
Antibacterianos , Antioxidantes , Celulose , Compostos de Cromo , Nanocompostos , Antibacterianos/administração & dosagem , Antibacterianos/química , Antioxidantes/administração & dosagem , Antioxidantes/química , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Compostos de Bifenilo/química , Catálise , Celulose/administração & dosagem , Celulose/química , Compostos de Cromo/administração & dosagem , Compostos de Cromo/química , Corantes/química , Violeta Genciana/química , Nanocompostos/administração & dosagem , Nanocompostos/química , Picratos/química , Poluentes Químicos da Água/química
11.
J Photochem Photobiol B ; 205: 111846, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32151785

RESUMO

Propolis has been reported to possess rich content of antioxidant compounds and may provide health benefits through oxidative stress reduction. Presently, the formulation activities used to enhance the drug delivery have been hampered due to inherently low aqueous solubility and poor transdermal permeation of the bioactive phenols and flavonoids. Here, we show, the formulation of propolis extract (PE) into phytosome delivery systems. The optimum antioxidant activity was attained through extraction process using 75% v/v ethanol. The phytosome was prepared using thin-layer hydration technique with l-α-Phosphatidylcholine as a phospholipid. Fourier transform infrared (FTIR) was used to investigate the occurrence of molecular interactions between formulation components. This innovative approach could encapsulate >40% of bioactive compounds in PE, namely caffeic acid, quercetin, and kaempferol. FTIR spectroscopy indicated new hydrogen bond formation, supporting successful phytosome formulation. The phytosomes enhanced the dissolution up to 4-folds of bioactive compounds in bio-mimicked release media, as well as improved penetrability and skin retention up to 6-folds of the three main compounds of propolis, when compared to non-encapsulated PE formulation. Importantly, the hydrogel containing phytosome showed a potential for UVA and UVB radiation absorption, indicated by the SPF values of higher than 15. To conclude, this work shows promising novel delivery approaches for PE in the treatment of organ injured stress oxidative and skin aging.


Assuntos
Antioxidantes , Portadores de Fármacos , Hidrogéis , Nanopartículas , Própole/química , Protetores contra Radiação , Animais , Antioxidantes/administração & dosagem , Antioxidantes/química , Compostos de Bifenilo/química , Portadores de Fármacos/administração & dosagem , Portadores de Fármacos/química , Liberação Controlada de Fármacos , Flavonoides/administração & dosagem , Flavonoides/análise , Hidrogéis/administração & dosagem , Hidrogéis/química , Técnicas In Vitro , Masculino , Nanopartículas/administração & dosagem , Nanopartículas/química , Fenóis/administração & dosagem , Fenóis/análise , Picratos/química , Protetores contra Radiação/administração & dosagem , Protetores contra Radiação/química , Ratos Sprague-Dawley , Pele/metabolismo , Absorção Cutânea , Fator de Proteção Solar , Raios Ultravioleta
12.
J Chromatogr A ; 1620: 460988, 2020 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-32115233

RESUMO

Traditional Chinese medicine (TCM), especially herbal medicine compound preparation, faces great challenges in its quality control due to a myriad of components involved. How to perform quality control of TCM more effectively has been a research topic. In this study, we used Tianmeng oral liquid (TOL) as a case study and developed a comprehensive strategy based on non-targeted, targeted and bioactive analyses for quality evaluation of TOL from different batches. Firstly, a non-targeted fingerprinting analysis was performed by HPLC-DAD and UHPLC-MS/MS. Twenty-five batches of TOL were clearly discriminated by similarity analysis and hierarchical cluster analysis and components were tentatively identified. Secondly, the targeted quantitative methods based on HPLC-DAD and HPLC-MS were applied to simultaneous quantitative determination of five and eight marker compounds, especially toxic component strychnine, respectively. The quantitative data were processed with principal component analysis for differentiating different batches of samples. Finally, we explored the feasibility of establishing a total antioxidant capacity (TAC) model. How to use the peak area instead of the corresponding concentration to determine the antioxidant activity-related compounds was theoretically explained for the first time, which was of great significance for the study of the fingerprint-efficacy relationship. The orthogonal signal correction-partial least squares model was employed to predict the TAC of TOL from their chromatographic fingerprints and identify three potential antioxidant markers. These results demonstrated that the comprehensive strategy from fingerprinting, chemical composition, multiple-component quantification, and antioxidant activity could be applied to quality evaluation of TOL and discrimination of the expired and unexpired samples.


Assuntos
Medicamentos de Ervas Chinesas/química , Medicina Tradicional Chinesa , Administração Oral , Compostos de Bifenilo/química , Calibragem , Cromatografia Líquida de Alta Pressão/métodos , Depuradores de Radicais Livres/química , Análise dos Mínimos Quadrados , Limite de Detecção , Modelos Teóricos , Picratos/química , Análise de Componente Principal , Padrões de Referência , Reprodutibilidade dos Testes , Soluções
13.
Molecules ; 25(4)2020 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-32079328

RESUMO

Pleurotus eryngii (DC:Fr.) Quel. is a cultivated mushroom of high culinary value and medicinal properties. Mycelium of P. eryngii is characterized by the ability of effective bio-elements absorption from growth media so it could be biofortified with trace elements with a functional activity in the human body. In this study, the ability of P. eryngii mycelia from in vitro cultures as well as fruiting bodies were investigated in terms of their effectiveness in zinc and selenium accumulation. The effect of Se and Zn biofortification on productivity, chemical compounds, and bio-elements content of P. eryngii was determined as well. To enhance Se and Zn content in P. eryngii fruiting bodies and mycelia, substrates were supplemented with sodium selenite, at a concentration of 50 mg L-1, zinc sulfate, and zinc hydro-aspartate at a concentration of 87.2 and 100.0 mg L-1, respectively. Mentioned Zn concentrations contained the same amount of zinc(II) ions, namely 20 mg L-1. The content of organic compounds include phenolic compounds and lovastatin, which were determined by a high-performance liquid chromatography with diode-array detector (HPLC-DAD) and reverse phase high-performance liquid chromatography (RP-HPLC) method with UV detection. The ability of P. eryngii to accumulate zinc and selenium from the culture medium was demonstrated. The degree of accumulation of zinc turned out to be different depending on the type of salt used. The present study also showed that conducting mycelium of P. eryngii in in vitro culture, with a higher content of zinc ions, can result in obtaining the materials with better antioxidant ability. The results of this study can be used to develop the composition of growing media, which ensures the production of biomass with the desired composition of elements.


Assuntos
Biofortificação , Carpóforos/química , Micélio/química , Pleurotus/química , Selênio/análise , Zinco/análise , Biomassa , Compostos de Bifenilo/química , Depuradores de Radicais Livres/química , Fenóis/análise , Picratos/química , Análise de Componente Principal
14.
Curr Pharm Biotechnol ; 21(10): 927-938, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32065101

RESUMO

BACKGROUND: The essential oil of methyl eugenol rich Cymbopogon khasianus Hack. was evaluated and its bioactivities were compared with pure methyl eugenol. So far, methyl eugenol rich essential oil of lemongrass was not studied for any biological activities; hence, the present study was conducted. OBJECTIVE: This study examined the chemical composition of essential oil of methyl eugenol rich Cymbopogon khasianus Hack., and evaluated its antioxidant, anti-inflammatory, antimicrobial, and herbicidal properties and genotoxicity, which were compared with pure compound, methyl eugenol. MATERIAL AND METHODS: Methyl eugenol rich variety of Cymbopogon khasianus Hack., with registration no. INGR18037 (c.v. Jor Lab L-9) was collected from experimental farm CSIR-NEIST, Jorhat, Assam (26.7378°N, 94.1570°E). The essential oil wasobtained by hydro-distillation using a Clevenger apparatus. The chemical composition of the essential oil was evaluated using GC/MS analysis and its antioxidant (DPPH assay, reducing power assay), anti-inflammatory (Egg albumin denaturation assay), and antimicrobial (Disc diffusion assay, MIC) properties, seed germination effect and genotoxicity (Allium cepa assay) were studied and compared with pure Methyl Eugenol compound (ME). RESULTS: Major components detected in the Essential Oil (EO) through Gas chromatography/mass spectroscopy analysis were methyl eugenol (73.17%) and ß-myrcene (8.58%). A total of 35components were detected with a total identified area percentage of 98.34%. DPPH assay revealed considerable antioxidant activity of methyl eugenol rich lemongrass essential oil (IC50= 2.263 µg/mL), which is lower than standard ascorbic acid (IC50 2.58 µg/mL), and higher than standard Methyl Eugenol (ME) (IC50 2.253 µg/mL). Methyl eugenol rich lemongrass EO showed IC50 38.00 µg/mL, ME 36.44 µg/mL, and sodium diclofenac 22.76 µg/mL, in in-vitro anti-inflammatory test. Moderate antimicrobial activity towards the 8 tested microbes was shown by methyl eugenol rich lemongrass essential oil whose effectiveness against the microbes was less as compared to pure ME standard. Seed germination assay further revealed the herbicidal properties of methyl eugenol rich essential oil. Moreover, Allium cepa assay revealed moderate genotoxicity of the essential oil. CONCLUSION: This paper compared the antioxidant, anti-inflammatory, antimicrobial, genotoxicity and herbicidal activities of methyl eugenol rich lemongrass with pure methyl eugenol. This methyl eugenol rich lemongrass variety can be used as an alternative of methyl eugenol pure compound. Hence, the essential oil of this variety has the potential of developing cost-effective, easily available antioxidative/ antimicrobial drugs but its use should be under the safety range of methyl eugenol and needs further clinical trials.


Assuntos
Cymbopogon/química , Eugenol/análogos & derivados , Óleos Voláteis/farmacologia , Folhas de Planta/química , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Compostos de Bifenilo/química , Aberrações Cromossômicas/efeitos dos fármacos , Cymbopogon/genética , Eugenol/isolamento & purificação , Eugenol/farmacologia , Eugenol/toxicidade , Fungos/efeitos dos fármacos , Cromatografia Gasosa-Espectrometria de Massas , Bactérias Gram-Positivas/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Óleos Voláteis/isolamento & purificação , Óleos Voláteis/toxicidade , Ovalbumina/imunologia , Picratos/química , Extratos Vegetais/química
15.
Pharm Biol ; 58(1): 184-199, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32083987

RESUMO

Context: Nephrotoxicity is a renal dysfunction that arises from direct exposure to environmental chemicals or as a side effect of therapeutic drugs. Boerhaavia diffusa Linn. (Nyctaginaceae), Rheum emodi Wall. Ex. Meissn. (Polygonaceae), Nelumbo nucifera Gaertn. (Nelumbonaceae) and Crataeva nurvala Buch-Ham. (Capparidaceae) are well-recognized medicinal plants of Indian traditional system of medicine used for kidney disorders.Objectives: The present investigation was undertaken to develop a chromatographically characterized polyherbal combination and to evaluate its nephroprotective activity.Materials and methods: Roots of B. diffusa and R. emodi, flowers of N. nucifera and stem bark of C. nurvala were extracted by decoction using 70% ethanol. Response surface methodology (RSM) was used for the optimization of extraction parameters. Polyherbal combinations with different doses (150-300 mg/kg) were tested against methotrexate-induced nephrotoxicity in Wistar rats.Results: The optimized extract contained 27% phenols and 15% flavonoids, which showed 75% 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging potential. Based on the retention time of high-performance liquid chromatography (HPLC) analysis, 17 out of 122 constituents were found common in all extracts and combinations. Two combinations showed significantly higher (p ≤ 0.05) DPPH scavenging potential and xanthine oxidase inhibition. The half maximal inhibitory concentration (IC50) of the best combination for DPPH scavenging and xanthine oxidase inhibition were 80 and 74 µg/mL, respectively. Treatment of methotrexate-induced nephrotoxic rats with polyherbal combination significantly (p ≤ 0.05) improved the kidney function markers, oxidative stress markers and histological parameters.Discussion and conclusion: The developed combination was found to be effective in nephrotoxicity; it can be explored further for the management of drug-induced nephrotoxicity and other chronic kidney diseases.


Assuntos
Antioxidantes/farmacologia , Nefropatias/prevenção & controle , Rim/efeitos dos fármacos , Metotrexato/toxicidade , Extratos Vegetais/farmacologia , Animais , Antioxidantes/química , Antioxidantes/isolamento & purificação , Compostos de Bifenilo/química , Simulação por Computador , Rim/metabolismo , Nefropatias/induzido quimicamente , Nefropatias/metabolismo , Masculino , Picratos/química , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Plantas Medicinais/química , Ratos Wistar , Xantina Oxidase/antagonistas & inibidores
16.
Molecules ; 25(4)2020 Feb 16.
Artigo em Inglês | MEDLINE | ID: mdl-32079080

RESUMO

In the last decade, there has been growing interest in the food industry in replacing synthetic chemicals with natural products with bioactive properties. This study's aims were to determine the chemical composition and the antioxidant properties of the essential oil of Pastianica sylvestris. The essential oil was isolated with a yield of 0.41% (w/v) by steam distillation from the dried seeds and subsequently analysed by GC-MS. Octyl acetate (78.49%) and octyl hexanoate (6.68%) were the main components. The essential oil exhibited an excellent activity for the inhibition of primary and secondary oxidation products for cold-pressed sunflower oil comparable with butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT), which were evaluated using peroxide and thiobarbituric acid values. The antioxidant activity of the essential oil was additionally validated using DPPH radical scavenging (0.0016 ± 0.0885 mg/mL), and ß-carotene-linoleic acid bleaching assays. Also, the amounts of total phenol components (0.0053 ± 0.0023 mg GAE/g) were determined.


Assuntos
Acetatos/química , Antioxidantes/química , Óleos Voláteis/química , Pastinaca/química , Sementes/química , Acetatos/isolamento & purificação , Antioxidantes/isolamento & purificação , Bioensaio , Compostos de Bifenilo/antagonistas & inibidores , Compostos de Bifenilo/química , Hidroxianisol Butilado/química , Hidroxianisol Butilado/isolamento & purificação , Hidroxitolueno Butilado/química , Cromatografia Gasosa-Espectrometria de Massas , Ácido Linoleico/química , Óleos Voláteis/isolamento & purificação , Fenóis/química , Picratos/antagonistas & inibidores , Picratos/química , Extratos Vegetais/química , Óleo de Girassol/química , Tiobarbitúricos/química , beta Caroteno/química
17.
Curr Pharm Biotechnol ; 21(11): 1070-1078, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32101118

RESUMO

INTRODUCTION: The plant, Astilboides tabularis (Hemsl.) Engler, is used in Chinese and Korean medicine to regulate blood sugar levels; however, little is known about its precise effects. MATERIALS AND METHODS: In this study, we aimed to measure the composition as well as the antioxidant, and anti-proliferative capacities of A. tabularis. Various extracts were generated using different organic solvents, and in vitro antioxidant activities were evaluated using DPPH free radical-scavenging and reducing power assays. The extracts were also evaluated based on their ability to inhibit lipopolysaccharide (LPS)-induced Nitric Oxide (NO) production in RAW 264.7 cells. RESULTS: Research shows that the A. tabularis ethyl acetate (EtOAc) extract showed significant antioxidant activity. Additionally, this extract could inhibit the LPS-induced expression of inflammatory mediators and pro-inflammatory cytokines in RAW 264.7 cells, including inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and interleukin-1 beta (IL-1ß). Notably, the A. tabularis EtOAc extract also displayed potent cytotoxic effects against MCF-7 and HeLa cancer cell lines, as determined by MTT assays. Lastly, total phenol and flavonoid content was measured for all extracts, and four flavonoid compounds-catechin, kaempferol, quercitrin, and isoquercetin were isolated from the EtOAc extract. Their structures were confirmed using mass spectrometry and nuclear magnetic resonance, and these isolated compounds were found to display potent DPPH free radical-scavenging activity. CONCLUSION: Thus, our data suggest that phenolic compounds in A. tabularis extracts promote antioxidant activity, and furthermore, these extracts show numerous features that indicate potential for therapeutic development.


Assuntos
Antineoplásicos/farmacologia , Antioxidantes/farmacologia , Proliferação de Células/efeitos dos fármacos , Óxido Nítrico/antagonistas & inibidores , Extratos Vegetais/farmacologia , Polifenóis/farmacologia , Saxifragaceae/química , Animais , Antineoplásicos/química , Antineoplásicos/isolamento & purificação , Antioxidantes/química , Antioxidantes/isolamento & purificação , Compostos de Bifenilo/química , Sobrevivência Celular/efeitos dos fármacos , Radicais Livres/química , Células HeLa , Humanos , Lipopolissacarídeos/farmacologia , Células MCF-7 , Camundongos , Óxido Nítrico/biossíntese , Óxido Nítrico Sintase Tipo II/antagonistas & inibidores , Picratos/química , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Polifenóis/química , Polifenóis/isolamento & purificação , Células RAW 264.7
18.
Molecules ; 25(3)2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-31979166

RESUMO

Two new xanthone derivatives, pestalotiones A (1) and B (2), one new diphenyl ketone riboside, pestalotione C (7), and one new diphenyl ether, pestalotione D (8), along with five known compounds isosulochrin dehydrate (3), 3,8-dihydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate (4), isosulochrin (5), chloroisosulochrin (6), and pestalotether D (9), were isolated from the crude extract of the plant endophytic fungus Pestalotiopsis theae (N635). The structures of the new compounds were unambiguously deduced by HRESIMS and 1D/2D-NMR spectroscopic data. Compound 6 showed modest cytotoxicity against the HeLa cell line with an IC50 value of 35.2 µM. Compound 9 also showed cytotoxic to the HeLa and MCF-7 cell lines, with IC50 values of 60.8 and 22.6 µM, respectively. Additionally, compounds 1 and 2 exhibited antioxidant activity in scavenging DPPH radical with IC50 values of 54.2 and 59.2 µg/mL, respectively.


Assuntos
Antioxidantes/química , Antioxidantes/farmacologia , Benzoatos/química , Compostos de Bifenilo/química , Sobrevivência Celular/efeitos dos fármacos , Endófitos , Células HeLa , Humanos , Hidrocarbonetos Clorados/química , Concentração Inibidora 50 , Células MCF-7 , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Picratos/química
19.
Food Funct ; 11(2): 1525-1536, 2020 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-31995080

RESUMO

Resveratrol (RES)-loaded protein-polysaccharide nanoparticles were fabricated through simple electrostatic interactions with oppositely charged α-lactalbumin (ALA) and chitosan (CHI) with a mass ratio of 5 : 1 without the addition of NaCl at pH 6.5. The Z-average diameter and zeta-potential values of RES-ALA-CHI nanoparticles were 211.0 nm and 13.23 mV, respectively. Both TEM and AFM graphs confirmed that RES-ALA-CHI nanoparticles had a spherical shape, and were dispersed homogeneously at the nanoscale. The encapsulation efficiency (EE) and loading amount (LA) of RES in RES-ALA-CHI nanoparticles were 58.86% and 196.2 µg mg-1, respectively, in the presence of 400 µg mL-1 RES. XRD results confirmed that RES was in amorphous form in ALA-CHI nanoparticles. The interaction between RES and ALA-CHI nanoparticles was mainly driven by hydrophobic interaction and hydrogen bonding. Compared to RES (free), the UV light and heat stability, in vitro bioaccessibility, and antioxidant activity of RES in RES-ALA-CHI nanoparticles were pronouncedly enhanced. The information provided in this study should be of interest to the food industry to fabricate robust nanoscale delivery systems with ALA-CHI nanoparticles for RES and other hydrophobic bioactive compounds.


Assuntos
Antioxidantes , Quitosana/química , Lactalbumina/química , Nanopartículas/química , Resveratrol , Antioxidantes/química , Antioxidantes/metabolismo , Compostos de Bifenilo/química , Compostos de Bifenilo/metabolismo , Portadores de Fármacos/química , Estabilidade de Medicamentos , Interações Hidrofóbicas e Hidrofílicas , Tamanho da Partícula , Picratos/química , Picratos/metabolismo , Resveratrol/química , Resveratrol/metabolismo
20.
Int J Biol Macromol ; 146: 444-452, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31923486

RESUMO

In this manuscript, lysozyme/κ-carrageenan (LYS-CRG) complexes were prepared and used to encapsulate curcumin. The LYS-CRG complexes demonstrate good encapsulation of curcumin (CUR), and the encapsulation efficiency (EE) and loading capacity (LC) reach 96.2% and 2.31%, respectively. The encapsulated CUR has high antioxidant activity, while the thermal stability and photostability of CUR are also increased. The LYS-CRG complexes could effectively improve the storage stability of CUR and increase its retention rate. In simulated gastric fluid, only 17.91% CUR in the CUR-LYS-CRG complex nanoparticles is released in 3 h, while in the simulated intestinal fluid, the CUR release rate quickly reaches 62.56% in 1.5 h. The release rate tends to be stable within 1.5 h to 3 h and the final release rate reaches 67.23%, suggesting that the formation of CUR-LYS-CRG complex nanoparticles does not affect CUR release in the simulated intestinal fluid.


Assuntos
Carragenina/química , Curcumina/farmacologia , Portadores de Fármacos/química , Liberação Controlada de Fármacos , Muramidase/metabolismo , Nanopartículas/química , Compostos de Bifenilo/química , Preparações de Ação Retardada , Emulsões/química , Depuradores de Radicais Livres/química , Luz , Nefelometria e Turbidimetria , Tamanho da Partícula , Picratos/química
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