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1.
Medicine (Baltimore) ; 99(9): e19370, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32118781

RESUMO

BACKGROUND: Knee osteoarthritis (KOA) is one of the most common chronic muscular diseases in old people. In recent years, people are more and more interested in the use of Chinese herbal medicine (CHM) in the treatment of KOA, such as kidney-tonifying and blood-activating medicinal herbs (KTBAMs) in the treatment of KOA. Many studies have confirmed that KTBAMs are effective in the treatment of KOA. However, it is still unknown whether KTBAMs and NSAIDs are more effective in the treatment of KOA. Therefore, we evaluated the efficacy and safety of KTBAMs and NSAIDs in the treatment of KOA. METHODS: Randomized controlled trials (RCTs) from online databases including PubMed, Embase, the Cochrane Library, China National Knowledge Infrastructure, Chinese Scientific Journal Database, Wanfang Data, and Chinese Biomedical Literature Database that compared the efficacy of KTBAMs and NSAIDs in the treatment of KOA were retrieved. The main outcomes included the evaluation of functional outcomes, pain and adverse effects. The Cochrane risk of bias (ROB) tool was used to assess methodological quality. RESULTS: The literature will provide a high-quality analysis of the current evidence supporting KTBAMs for KOA based on various comprehensive assessments including the total effective rate, visual analog scale scores, Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), Lequence scores, Knee Society Scale (KSS) scores, and adverse effects. CONCLUSION: This proposed systematic review will provide up-to-date evidence to assess the effect of KTBAMs in the treatment for patients with KOA. RESEARCH REGISTRY REGISTRATION NUMBER: : reviewregistry 783.


Assuntos
Anti-Inflamatórios não Esteroides/normas , Osteoartrite do Joelho/tratamento farmacológico , Plantas Medicinais/metabolismo , Anti-Inflamatórios não Esteroides/uso terapêutico , Protocolos Clínicos , Humanos , Rim/efeitos dos fármacos , Osteoartrite do Joelho/fisiopatologia
2.
Ecotoxicol Environ Saf ; 193: 110342, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32109585

RESUMO

Agricultural production of Ligusticum chuanxiong Hort. is often affected by heavy metal pollution in soil, especially mixtures of cadmium (Cd) and lead (Pb). We assessed metal-induced phytotoxicity in L. chuanxiong by exposing the plants to soil treated with Cd, Pb, or Cd/Pb mixtures. A combined Cd/Pb treatment alleviated the inhibition in plant growth, photosynthesis, and secondary metabolite generation seen in single-metal exposures in three of the four combinations. Most combined Cd/Pb treatments resulted in preferential uptake of magnesium, copper, and nitrogen in underground plant parts and accumulation of phosphorus and calcium in aboveground plant parts, thereby leading to improvements in photosynthetic potential. Compared with single-metal exposures, combined Cd/Pb treatment significantly decreased the contents of Cd by 16.67%-40.12% and Pb by 10.68%-21.70% in the plant, respectively. At the subcellular level, the Pb presence increased the Cd percentage associated with cell wall from 64.79% to 67.93% in rhizomes and from 32.76% to 45.32% in leaves, while Cd reduced Pb contents by 9.36%-46.39% in the subcellular fractions. A combined Cd/Pb treatment decreased the contents of water- and ethanol-extractable metal forms and increased the contents of acetic acid- and hydrochloric acid-extractable forms. The lower toxic effects of the Cd/Pb mixture in L. chuanxiong were associated with photosynthetic potential, subcellular distribution, the chemical forms of Cd and Pb, and synthesis of secondary metabolites. These findings are useful for plant production strategies in soils contaminated by heavy metals.


Assuntos
Cádmio/toxicidade , Chumbo/toxicidade , Ligusticum/efeitos dos fármacos , Poluentes do Solo/toxicidade , Cádmio/farmacocinética , Cálcio/metabolismo , Cobre/metabolismo , Interações Medicamentosas , Chumbo/farmacocinética , Ligusticum/metabolismo , Magnésio/metabolismo , Nitrogênio/metabolismo , Fósforo/metabolismo , Fotossíntese/efeitos dos fármacos , Folhas de Planta/metabolismo , Plantas Medicinais/efeitos dos fármacos , Plantas Medicinais/metabolismo , Metabolismo Secundário/efeitos dos fármacos , Poluentes do Solo/farmacocinética
3.
PLoS One ; 15(2): e0229490, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32107496

RESUMO

Application of plant growth regulators has become one of the most important means of improving yield and quality of medicinal plants. To understand the molecular basis of phytohormone-regulated oleanolic acid metabolism, RNA-seq was used to analyze global gene expression in Achyranthes bidentata treated with 2.0 mg/L 1-naphthaleneacetic acid (NAA) and 1.0 mg/L 6-benzyladenine (6-BA). Compared with untreated controls, the expression levels of 20,896 genes were significantly altered with phytohormone treatment. We found that 13071 (62.5%) unigenes were up-regulated, and a lot of differentially expressed genes involved in hormone or terpenoid biosynthesis, or transcription factors were significantly up-regulated. These results suggest that oleanolic acid biosynthesis induced by NAA and 6-BA occurs due to the expression of key genes involved in jasmonic acid signal transduction. This study is the first to analyze the production and hormonal regulation of medicinal A. bidentata metabolites at the molecular level. The results herein contribute to a better understanding of the regulation of oleanane-type triterpenoid saponins accumulation and define strategies to improve the yield of these useful metabolites.


Assuntos
Achyranthes/efeitos dos fármacos , Achyranthes/metabolismo , Compostos de Benzil/farmacologia , Ciclopentanos/metabolismo , Ácidos Naftalenoacéticos/farmacologia , Ácido Oleanólico/biossíntese , Oxilipinas/metabolismo , Purinas/farmacologia , Achyranthes/crescimento & desenvolvimento , Vias Biossintéticas/efeitos dos fármacos , Vias Biossintéticas/genética , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Genes de Plantas , Medicina Tradicional Chinesa , Filogenia , Reguladores de Crescimento de Planta/metabolismo , Plantas Medicinais/efeitos dos fármacos , Plantas Medicinais/genética , Plantas Medicinais/metabolismo , RNA-Seq , Saponinas/metabolismo
4.
Nat Commun ; 11(1): 971, 2020 02 20.
Artigo em Inglês | MEDLINE | ID: mdl-32080175

RESUMO

Triptolide is a trace natural product of Tripterygium wilfordii. It has antitumor activities, particularly against pancreatic cancer cells. Identification of genes and elucidation of the biosynthetic pathway leading to triptolide are the prerequisite for heterologous bioproduction. Here, we report a reference-grade genome of T. wilfordii with a contig N50 of 4.36 Mb. We show that copy numbers of triptolide biosynthetic pathway genes are impacted by a recent whole-genome triplication event. We further integrate genomic, transcriptomic, and metabolomic data to map a gene-to-metabolite network. This leads to the identification of a cytochrome P450 (CYP728B70) that can catalyze oxidation of a methyl to the acid moiety of dehydroabietic acid in triptolide biosynthesis. We think the genomic resource and the candidate genes reported here set the foundation to fully reveal triptolide biosynthetic pathway and consequently the heterologous bioproduction.


Assuntos
Sistema Enzimático do Citocromo P-450/genética , Sistema Enzimático do Citocromo P-450/metabolismo , Diterpenos/metabolismo , Fenantrenos/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Tripterygium/genética , Tripterygium/metabolismo , Abietanos/metabolismo , Antineoplásicos Fitogênicos/biossíntese , Vias Biossintéticas/genética , Medicamentos de Ervas Chinesas/metabolismo , Compostos de Epóxi/metabolismo , Perfilação da Expressão Gênica , Genoma de Planta , Humanos , Engenharia Metabólica , Metaboloma , Oxirredução , Filogenia , Plantas Medicinais/genética , Plantas Medicinais/metabolismo
5.
PLoS One ; 15(2): e0220084, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32074105

RESUMO

Bletilla striata is an endangered traditional Chinese medicinal plant with multiple uses and a slow regeneration rate of its germplasm resources. To evaluate the callus growth kinetics and accumulation of secondary metabolites (SMs), a callus suspension culture was proven to be a valuable approach for acquiring high yields of medicinal compounds. An effective callus suspension culture for obtaining B. striata callus growth and its SMs was achieved with the in vitro induction of calluses from B. striata seeds. The callus growth kinetics and accumulation of SMs were analyzed using a mathematical model. The resulting callus growth kinetic model revealed that the growth curves of B. striata suspension-cultured calluses were sigmoidal, indicating changes in the growth of the suspension-cultured calluses. Improved Murashige and Skoog callus growth medium was the most favorable medium for B. striata callus formation, with the highest callus growth occurring during the stationary phase of the cultivation period. Callus growth acceleration started after 7 days and thereafter gradually decreased until day 24 of the cultivation period and reached its highest at day 36 period in both the dry weight and fresh weight analyses. The coelonin concentration peaked during the exponential growth stage and decreased afterward during the stationary stage of the callus suspension culture. The maximum content of coelonin (approximately 0.3323 mg/g callus dry weight) was observed on the 18th day of the cultivation cycle, while dactylorhin A and militarine reached the highest concentrations at day 24, and p-hydroxybenzyl alcohol at day 39. This investigation also laid a foundation for a multimathematical model to better describe the accumulation variation of SMs. The production of SMs showed great specificity during callus growth and development. This research provided a well-organized way to increase the accumulation and production of SMs during the scaled-up biosynthesis of calluses in B. striata callus suspension cultures.


Assuntos
Calo Ósseo/crescimento & desenvolvimento , Técnicas de Cultura de Células/métodos , Plantas Medicinais/metabolismo , Glucosídeos/análise , Cinética , Modelos Teóricos , Metabolismo Secundário , Sementes/química , Succinatos/análise
6.
Chemosphere ; 247: 125928, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32069718

RESUMO

The contamination and distribution of mercury and selenium in the Chinese medicinal fungus Wolfiporia cocos was investigated. The sclerotial mercury concentrations ranged from 0.0043 to 0.027 mg kg1 dry biomass (db) in the inner white part and 0.019-0.074 mg kg-1 db in the shell (outer part), while selenium concentrations ranged from < 0.00048 to 0.0040 mg kg-1 db (white) and 0.0034-0.038 mg kg-1 db (shell). Positive correlations were found for mercury, as well as for mercury and selenium but they were not consistent for both morphological parts. Mercury concentrations exceeded selenium in 16 of 17 white part pools (molar quotient 0.53 to > 10) and in 11 of 17 shell pools (quotient 0.37 to 3.2). The estimated maximal exposure to mercury contained in sclerotial products based on 45 g per capita daily intake for a 60 kg individual over one week, was 0.000020 mg kg-1 body mass (bm; white) and 0.000055 mg kg-1 bm (shell) on a daily basis, and 0.00014 mg kg-1 bm (white) and 0.00039 mg kg-1 bm (shell) on a weekly basis. Relative to mercury, the corresponding intake rates of selenium were considered very low, i.e., they averaged on a daily basis at 0.00075 µg kg-1 bm (white) and 0.0097 µg kg-1 bm (shell) with maximum intake at 0.0030 µg kg-1 bm (white) and 0.028 µg kg-1 bm (shell).


Assuntos
Poluentes Ambientais/metabolismo , Mercúrio/metabolismo , Selênio/metabolismo , Wolfiporia/metabolismo , China , Poluentes Ambientais/análise , Humanos , Mercúrio/análise , Plantas Medicinais/química , Plantas Medicinais/metabolismo , Selênio/análise , Wolfiporia/química
7.
Plant Mol Biol ; 102(4-5): 477-499, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31902069

RESUMO

KEY MESSAGE: The study carry out comprehensive transcriptome analysis of C. deltoidea and exploration of BIAs biosynthesis and accumulation based on UHPLC-MS/MS and combined sequencing platforms. Coptis deltoidea is an important medicinal plant with a long history of medicinal use, which is rich in benzylisoquinoline alkaloids (BIAs). In this study, Ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) and combined sequencing platforms were performed for exploration of BIAs biosynthesis, accumulation and comprehensive transcriptome analysis of C. deltoidea. By metabolism profiling, the accumulation of ten BIAs was analyzed using UHPLC-MS/MS and different contents were observed in different organs. From transcriptome sequencing result, we applied single-molecule real-time (SMRT) sequencing to C. deltoidea and generated a total of 75,438 full-length transcripts. We proposed the candidate biosynthetic pathway of tyrosine, precursor of BIAs, and identified 64 full length-transcripts encoding enzymes putatively involved in BIAs biosynthesis. RNA-Seq data indicated that the majority of genes exhibited relatively high expression level in roots. Transport of BIAs was also important for their accumulation. Here, 9 ABC transporters and 2 MATE transporters highly homologous to known alkaloid transporters related with BIAs transport in roots and rhizomes were identified. These findings based on the combined sequencing platforms provide valuable genetic information for C. deltoidea and the results of transcriptome combined with metabolome analysis can help us better understand BIAs biosynthesis and transport in this medicinal plant. The information will be critical for further characterization of C. deltoidea transcriptome and molecular-assisted breeding for this medicinal plant with scarce resources.


Assuntos
Alcaloides/biossíntese , Benzilisoquinolinas/química , Coptis/genética , Coptis/metabolismo , Transcriptoma , Cromatografia Líquida de Alta Pressão , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Filogenia , Folhas de Planta/metabolismo , Raízes de Plantas/metabolismo , Plantas Medicinais/metabolismo , Rizoma/metabolismo , Análise de Sequência de DNA , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas em Tandem
8.
Plant Physiol Biochem ; 148: 80-89, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31951944

RESUMO

Secondary metabolites (SMs) of medicinal plants are the material basis of their clinically curative effects. They are also important indicators for evaluating the quality of medicinal materials. However, the synthesis and accumulation of SMs are very complex, which are affected by many factors including internal developmental genetic circuits (regulated gene, enzyme) and by external environment factors (light, temperature, water, salinity, etc.). Currently, lots of literatures focused on the effect of environmental factors on the synthesis and accumulation of SMs of medicinal plants, the effect of the developmental growth and genetic factors on the synthesis and accumulation of SMs still lack systematic classification and summary. Here, we have given the review base on our previous works on the morphological development of medicinal plants and their secondary metabolites, and systematically outlined the literature reports how different environmental factors affected the synthesis and accumulation of SMs. The results of our reviews can know how developmental and environmental factors qualitatively and quantitatively influence SMs of medicinal plants and how these can be integrated as tools to quality control, as well as on the improvement of clinical curative effects by altering their genomes, and/or growth conditions.


Assuntos
Meio Ambiente , Plantas Medicinais , Plantas Medicinais/química , Plantas Medicinais/crescimento & desenvolvimento , Plantas Medicinais/metabolismo , Salinidade , Temperatura , Água
9.
Bull Environ Contam Toxicol ; 104(3): 373-379, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31955229

RESUMO

Chlorophyll content in leaves is a convenient indicator of the physiological state of the plant following exposure to a stressor. In the present study, a pot experiment was carried out using two medicinal plant species - peppermint (Mentha X piperita L.) and common sage (Salvia officinalis L.) to determine the link between the chlorophyll content in the plant leaves and the exposure of the plants to nano-TiO2 either through the leaves (in suspension) or through the root system (in soil). Following the exposure, the shoots were analysed for the contents of Ti, Al, Ca, K, Mg, Mn, Na and chlorophyll. Significant decrease in chlorophyll content was observed in all but one of the nano-TiO2 treatments, the differences in the determined element content were mostly species-dependent: Ti exposure was found to be related to the decrease in the chlorophyll and Mn content in both species as well as to the decrease in Mg content in common sage.


Assuntos
Clorofila/metabolismo , Plantas Medicinais/metabolismo , Titânio/farmacologia , Antioxidantes , Mentha piperita , Extratos Vegetais , Folhas de Planta/metabolismo , Raízes de Plantas , Salvia officinalis , Sódio/análise , Titânio/análise
10.
J Photochem Photobiol B ; 203: 111761, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31896050

RESUMO

The combination of different colors from light-emitting diodes (LEDs) may influence growth and production of secondary metabolites in plants. In the present study, the effect of light quality on morphophysiology and content of 20-hydroxyecdysone (20E), a phytoecdysteroid, was evaluated in accessions of an endangered medicinal species, Pfaffia glomerata, grown in vitro. Two accessions (Ac22 and Ac43) were cultured in vitro under three different ratios of red (R) and blue (B) LEDs: (i) 1R:1B, (ii) 1R:3B, and (iii) 3R:1B. An equal ratio of red and blue light (1R:1B) increased biomass accumulation, anthocyanin content, and 20E production (by 30-40%). Moreover, 1R:1B treatment increased the size of vascular bundles and vessel elements, as well as strengthened xylem lignification and thickening of the cell wall of shoots. The 1R:3B treatment induced the highest photosynthetic and electron transport rates and enhanced the activity of oxidative stress-related enzymes. Total Chl content, Chl/Car ratio, and NPQ varied more by accession type than by light source. Spectral quality affected primary metabolism differently in each accession. Specifically, in Ac22 plants, fructose content was higher under 1R:1B and 1R:3B treatments, whereas starch accumulation was higher under 1R:3B, and sucrose under 3R:1B. In Ac43 plants, sugars were not influenced by light spectral quality, but starch content was higher under 3R:1B conditions. In conclusion, red and blue LEDs enhance biomass and 20E production in P. glomerata grown in vitro.


Assuntos
Amaranthaceae/efeitos da radiação , Ecdisterona/análise , Luz , Amaranthaceae/crescimento & desenvolvimento , Amaranthaceae/metabolismo , Antocianinas/análise , Biomassa , Carotenoides/análise , Catalase/metabolismo , Clorofila/análise , Cromatografia Líquida de Alta Pressão , Folhas de Planta/química , Proteínas de Plantas/metabolismo , Raízes de Plantas/química , Raízes de Plantas/crescimento & desenvolvimento , Caules de Planta/química , Plantas Medicinais/crescimento & desenvolvimento , Plantas Medicinais/metabolismo , Plantas Medicinais/efeitos da radiação , Amido/metabolismo , Superóxido Dismutase/metabolismo
11.
J Ethnopharmacol ; 246: 112158, 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31421182

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Sri Lanka is known to have very diverse flora. Many of these species are used for plant-based remedies, which form the integral part of two Sri Lankan systems of traditional medicine, Ayurveda and Deshiya Chikitsa. Despite their widespread use, only a limited number of studies have probed into the scientific evidence for bioactivity of these medicinal plants. Such studies rarely progress to the identification of bioactive natural products. AIM OF THE STUDY: The primary aim was to develop a bioactivity screening method and apply it to 50 Sri Lankan medicinal plants where antimicrobial properties could be relevant for its traditional use. The subsequent aim was the progression into defining and characterising potent isolates within targeted compound classes from such plants, i.e. Derris scandens and its antimicrobial flavonoids. MATERIAL AND METHODS: The plant collection comprised 24 species of Fabaceae, 15 Rubiaceae, 7 Solanaceae and 4 Cucurbitaceae plants. These 50 species were collected based on their ethnopharmacological importance and use in Sri Lankan traditional medicine. Crude extracts from each species were initially subjected to radial disc diffusion and microdilution assays. Subsequently, aqueous extracts of all plants were microfractionated in deep well plates using reversed-phase HPLC. Fractions were tested for antibacterial and cytotoxic activities and masses of target bioactive compounds were identified using mass spectrometry. Bioactive compounds with the masses identified through microfractions were isolated from Derris scandens using reversed-phase HPLC. The isolated pure compounds were characterised using LC-MS and NMR. RESULTS: Crude aqueous extracts from 19 species showed activity against Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) in the radial disc diffusion assay. Crude aqueous extracts from 34 plant species and organic extracts from 46 plant species were active against S. aureus (≤4 mg mL-1) in the microdilution assay. Microfractionation demonstrated antibacterial activity for 19 plants and cytotoxicity for 6 plants. Furthermore, target bioactive compounds and their molecular ions were identified during microfractionation. Dalpanitin and vicenin-3, two of the flavonoids isolated from Derris scandens gave MICs of 23 µg mL-1 against S. aureus. Dalpanitin also exhibited relevant MICs on Gram-negative bacteria (94 µg mL-1 against Escherichia coli and Pseudomonas aeruginosa). CONCLUSION: The microfractionation protocol developed in this study enabled time-efficient screening of many plants species, using a small quantity of sample material. In addition, microfractionation served as a guiding tool for identifying individual antimicrobial compounds. Through this process, flavonoids were isolated from Derris scandens, out of which dalpanitin and vicenin-3 showed activity in the low micromolar range. The high hit rate for in vitro antibacterial properties from this ethnopharmacologically guided sample collection gives credence to Sri Lankan traditional herbal medicine as a source for drug discovery.


Assuntos
Antibacterianos/isolamento & purificação , Flavonoides/isolamento & purificação , Magnoliopsida/metabolismo , Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Fracionamento Químico , Flavonoides/farmacologia , Humanos , Extratos Vegetais/química , Plantas Medicinais/metabolismo , Metabolismo Secundário , Sri Lanka
12.
Food Chem ; 309: 125715, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31685368

RESUMO

Rhodomyrtus tomentosa (Aiton) is a flowering plant native to southern and southeastern Asia. Up to date, 106 chemical constituents have been isolated and identified from R. tomentosa. Among these compounds, triterpenoids, flavonoids, phenols and meroterpenoids are the major constituents. Investigations of pharmacological activities of R. tomentosa revealed that this edible medicinal herb exhibits a wide range of therapeutic potential including antibacterial, antitumor, anti-inflammatory and antioxidant activities both in vivo and in vitro. The purpose of this review is to provide an overview of R. tomentosa studies until 2019. This article also intends to review advances in the botanical, phytochemical, pharmacological studies and industrial applications of R. tomentosa, which will provide a useful bibliography for further investigations and applications of R. tomentosa in medicines and foods.


Assuntos
Myrtaceae/química , Compostos Fitoquímicos/química , Antibacterianos/química , Antibacterianos/farmacologia , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Antioxidantes/química , Sobrevivência Celular/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Humanos , Myrtaceae/metabolismo , Compostos Fitoquímicos/farmacologia , Plantas Medicinais/química , Plantas Medicinais/metabolismo
13.
Pak J Pharm Sci ; 32(4): 1555-1561, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31608874

RESUMO

To analyse metabolic compounds of Justicia adathoda to evaluate against pathogens, inflammation and cervical cancer. The investigation exposed that the extracts of Justicia adathoda have potent metabolic to eradicate the human diseases. The antibacterial, tumorolytic and anti-inflammatory activity of ethyl acetate and aqueous extracts of Justicia adathoda (leaves) were assessed. In vitro anti-inflammatory activity was assessed by standard procedures. Justicia adathoda metabolic exhibit anticancer activity in human cervical cancer cell line (HeLa) (in vitro) analysis. Flavonoids, saponins, alkaloids, amino acids, tannins and terpenoids were present in both the extracts. The active components present in the extracts were found to be amino acids, alkaloids, lipids and triterpenoids which have antibacterial activity shows inhibition against Salmonella and Escherichia coli. Justicia adathoda possesses significant anti-inflammatory activity and it was confirmed by in-vitro analysis. The anticancer activity was found effective in human cervical cancer cell line (HeLa) (in-vitro) analysis. From the investigation could conclude that the metabolic compounds Justicia adathoda is effective against Anti-inflammation and ethyl acetate extract of Justicia adathoda are effective for Cancer.


Assuntos
Antibacterianos/farmacologia , Anti-Inflamatórios não Esteroides/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Adhatoda/química , Extratos Vegetais/farmacologia , Antibacterianos/química , Anti-Inflamatórios não Esteroides/química , Antineoplásicos Fitogênicos/química , Avaliação Pré-Clínica de Medicamentos , Escherichia coli/efeitos dos fármacos , Células HeLa , Humanos , Hialuronoglucosaminidase/antagonistas & inibidores , Adhatoda/metabolismo , Compostos Fitoquímicos/análise , Extratos Vegetais/química , Plantas Medicinais/química , Plantas Medicinais/metabolismo
15.
Anal Chim Acta ; 1088: 89-98, 2019 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-31623720

RESUMO

The principle of chromatographic fingerprint is that certain diagnostic metabolites should be always distributed in a given plant and currently, it has been widely accepted as a promising means for medicinal plant authentication. Moreover, the chemical profile is the only evidence to clarify the ingredients of those consumable plant products, e.g. traditional Chinese medicine (TCM) prescriptions. Herein, efforts were made to describe the diagnostic metabolome of medicinal plant or TCM prescription using a binary code sequence. Forty-five well-known medicinal plants along with six relevant prescriptions were employed for concept illustration and proof. Each plant was subjected to chemical characterization, and diagnostic metabolites of all plants were gathered into a chemical pool containing 595 compounds. A robust method enabling the detection of all 595 constituents was then developed using LC coupled to scheduled multiple reaction monitoring. Analyst™ software was responsible for automatically judging the presence (defined as "1") or absence (defined as "0") of each analyte with a defined signal-to-noise threshold (S/N > 100). After converting each medicinal plant to a binary sequence consisting of 595 codes, an in-house database was built by involving all sequences. The potentials of sequence library retrieval towards plant authentication, preliminary chemical characterization, and deformulation of TCM prescriptions were demonstrated after that the diagnostic metabolome of each test sample was translated to a binary code sequence. Above all, binary code is a flexible tool for diagnostic metabolite sequencing of medicinal plants, and it should be an alternative tool of DNA barcoding towards plant authentication.


Assuntos
Metabolômica/métodos , Plantas Medicinais/química , Plantas Medicinais/metabolismo , Métodos Analíticos de Preparação de Amostras , Composição de Medicamentos , Prescrições de Medicamentos , Fraude/prevenção & controle , Limite de Detecção , Medicina Tradicional Chinesa
16.
Chem Biol Interact ; 314: 108844, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31600484

RESUMO

Using data from Schink et al. (2018), a large number of herbal extracts were assessed for their capacity to induce pro- and anti-inflammatory effects based on TLR4 expression normalized for cell viability in two immune cell models (i.e., HeLa-TLR4 transfected reporter cell line, and THP-1 monocytes) applying seven concentrations (0.01-3.0%). The analysis revealed that 70-80% of the extracts satisfying the a priori entry criteria also satisfied a priori evaluative criteria for hormetic concentration responses. These findings demonstrate that a large proportion of herbal extracts display hormetic dose responses in immune cells, indicating that hormetic mechanisms mediate pro- and anti-inflammatory processes and may provide a means to guide optimal dosing strategies. The identification of doses eliciting only anti-inflammatory therapeutic activity as well as the use of dose-variable herbal extracts in the treatment of inflammatory diseases will be challenging.


Assuntos
Anti-Inflamatórios/farmacologia , Hormese/efeitos dos fármacos , Extratos Vegetais/química , Plantas Medicinais/química , Anti-Inflamatórios/química , Linhagem Celular , Células HeLa , Humanos , Monócitos/citologia , Monócitos/efeitos dos fármacos , Monócitos/metabolismo , Extratos Vegetais/farmacologia , Plantas Medicinais/metabolismo , Receptor 4 Toll-Like/genética , Receptor 4 Toll-Like/metabolismo
17.
Carbohydr Polym ; 226: 115304, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31582069

RESUMO

Medicinal herb Bletilla striata as a traditional Chinese herb has been used to treat alimentary canal mucosal damage, ulcers, bruises, and burns for thousands of years. Despite numerous efforts directed at the development of Bletilla striata products, the challenge of preparing Bletilla striata hemostasis dressings while simultaneously maintaining portability and high hemostasis performance has not yet been addressed. Herein, we describe a Bletilla striata polysaccharide (BSP) sponge with hierarchical aligned porous channels formed via directional freeze technology. The microstructure and mechanical property of the BSP sponges could be controlled by modifying the BSP concentration. Notably, in vivo animal studies indicated that BSP sponges show high biocompatibility and degradation and possess excellent hemostasis capability. In addition, BSP sponges showed accelerated wound healing in comparison to commercial dressings. Thus, our study indicates that the constructed BSP sponges could find potential application in dressings for efficient wound healing.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Orchidaceae/metabolismo , Plantas Medicinais/metabolismo , Polissacarídeos/farmacologia , Animais , Bandagens , Materiais Biocompatíveis/farmacologia , Feminino , Hemostasia/efeitos dos fármacos , Masculino , Camundongos Endogâmicos C57BL , Coelhos , Cicatrização
19.
Int J Mol Sci ; 20(18)2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31505762

RESUMO

Scutellaria baicalensis is a well-known medicinal plant that produces biologically active flavonoids, such as baicalin, baicalein, and wogonin. Pharmacological studies have shown that these compounds have anti-inflammatory, anti-bacterial, and anti-cancer activities. Therefore, it is of great significance to investigate the genetic information of S. baicalensis, particularly the genes related to the biosynthetic pathways of these compounds. Here, we constructed the full-length transcriptome of S. baicalensis using a hybrid sequencing strategy and acquired 338,136 full-length sequences, accounting for 93.3% of the total reads. After the removal of redundancy and correction with Illumina short reads, 75,785 nonredundant transcripts were generated, among which approximately 98% were annotated with significant hits in the protein databases, and 11,135 sequences were classified as lncRNAs. Differentially expressed gene (DEG) analysis showed that most of the genes related to flavonoid biosynthesis were highly expressed in the roots, consistent with previous reports that the flavonoids were mainly synthesized and accumulated in the roots of S. baicalensis. By constructing unique transcription models, a total of 44,071 alternative splicing (AS) events were identified, with intron retention (IR) accounting for the highest proportion (44.5%). A total of 94 AS events were present in five key genes related to flavonoid biosynthesis, suggesting that AS may play important roles in the regulation of flavonoid biosynthesis in S. baicalensis. This study provided a large number of highly accurate full-length transcripts, which represents a valuable genetic resource for further research of the molecular biology of S. baicalensis, such as the development, breeding, and biosynthesis of active ingredients.


Assuntos
DNA Complementar , Regulação da Expressão Gênica/fisiologia , Sequenciamento de Nucleotídeos em Larga Escala , Raízes de Plantas , Plantas Medicinais , Scutellaria baicalensis , DNA Complementar/genética , DNA Complementar/metabolismo , Raízes de Plantas/genética , Raízes de Plantas/metabolismo , Plantas Medicinais/genética , Plantas Medicinais/metabolismo , Scutellaria baicalensis/genética , Scutellaria baicalensis/metabolismo
20.
Chem Biodivers ; 16(12): e1900459, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31535771

RESUMO

This study was conducted to analyze the bioactive compounds and in vitro antioxidant capacity of tea infusions prepared from whole and ground medicinal fruits, including gardenia, jujube, magnolia, quince, and wolfberries. The dried medicinal fruit samples were ground, and then passed through a 60-mesh sieve (pore size, 250 µm). Hot water (80 °C) infusions of whole and ground fruits were examined. In average of both whole and ground tea infusions, the maximum bioactive compounds were found in gardenia (ß-carotene, lycopene, and vitamin C), magnolia (total chlorophyll and anthocyanin), quince (flavonoid), and wolfberries (phenolic), and the maximum antioxidant capacity was found in quince (ABTS and DPPH) and wolfberries (NSA). Whole fruit tea infusions showed a higher brightness than the ground fruit tea infusions. The total chlorophyll, anthocyanin, ß-carotene, lycopene, phenolic, flavonoid, and vitamin C contents were found to be significantly (p≤0.001) higher in the ground fruit tea infusions than in the whole fruit tea infusions; additionally, the ground fruit tea infusions had a higher antioxidant capacity especially ABTS, DPPH and NSA. Therefore, the ground fruit tea infusions appeared to be more powerful with regard to the contents of bioactive compounds and antioxidant capacities than the whole fruit tea infusions.


Assuntos
Antioxidantes/química , Plantas Medicinais/química , Chá/química , Antocianinas/análise , Clorofila/química , Flavonoides/análise , Frutas/química , Frutas/metabolismo , Temperatura Alta , Licopeno/análise , Fenóis/análise , Extratos Vegetais/química , Plantas Medicinais/metabolismo , beta Caroteno/análise
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