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1.
Sensors (Basel) ; 21(10)2021 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-34065481

RESUMO

Human epididymis protein 4 (HE4) is an ovarian cancer marker. Various cut-off values of the marker in blood are recommended, depending on the method used for its determination. An alternative biosensor for HE4 determination in blood plasma has been developed. It consists of rabbit polyclonal antibody against HE4, covalently attached to a gold chip via cysteamine linker. The biosensor is used with the non-fluidic array SPRi technique. The linear range of the analytical signal response was found to be 2-120 pM, and the biosensor can be used for the determination of the HE4 marker in the plasma of both healthy subjects and ovarian cancer patients after suitable dilution with a PBS buffer. Precision (6-10%) and recovery (101.8-103.5%) were found to be acceptable, and the LOD was equal to 2 pM. The biosensor was validated by the parallel determination of a series of plasma samples from ovarian cancer patients using the Elecsys HE4 test and the developed biosensor, with a good agreement of the results (a Pearson coefficient of 0.989). An example of the diagnostic application of the developed biosensor is given-the influence of ovarian tumor resection on the level of HE4 in blood serum.


Assuntos
Técnicas Biossensoriais , Neoplasias Ovarianas , Proteína 2 do Domínio Central WAP de Quatro Dissulfetos/análise , Biomarcadores Tumorais/análise , Feminino , Humanos , Neoplasias Ovarianas/diagnóstico , Plasma
2.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(3): 988-992, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34105505

RESUMO

Novel coronavirus disease 2019 (COVID-19) has started to erupt in Wuhan since December 2019, and then the cases of COVID-19 increased rapidly, the epidemic situation spreaded to most area of China. Owing to the lack of specific drugs at present, convalescent plasma therapy becomes an alternative treatment. However, the choice of the donor, suitable patients, timing of treatment, administrate dose, the advantages and disadvantages of the treatment are not clear. This article reviews the clinical studies and case reports of convalescent plasma therapy in several previous viral infectious diseases in order to provide clues for the treatment of COVID-19.


Assuntos
COVID-19 , Doenças Transmissíveis , Transfusão de Componentes Sanguíneos , COVID-19/terapia , China , Humanos , Imunização Passiva , Plasma , SARS-CoV-2
3.
Molecules ; 26(10)2021 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-34063409

RESUMO

Brahmi essence, developed from Bacopa monnieri (L.) Wettst. standardized extract and mulberry juice, was proven to improve the memory speed of healthy participants aged 55-80 years old, following a 12-week dietary program. However, the metabolites have not yet been reported. Our objective was to characterize the altered metabolites in the plasma, urine, and feces of healthy volunteers after consumption of Brahmi essence for 12 weeks, using the LC-MS metabolomics approach. The altered metabolites were selected from OPLS-DA S-plots; 15 metabolites in the plasma, 7 in the urine, and 17 in the feces samples were tentatively identified by comparison with an online database and literature. The metabolites in the plasma samples were in the classes of amino acids, acylcarnitine, and phospholipids. Benzeneactamide-4-O-sulphate and 3-hydroxyhippuric acid were found in urine samples. The metabolites in the class of amino acids, together with jujubogenin and pseudojujubogenin, were identified in the fecal samples. The aminoacyl-tRNA, aromatic amino acids, and branched-chain amino acid biosynthetic pathways were mainly related to the identified metabolites in all three samples. It could be implied that those metabolites and their pathways might be linked with the effect of Brahmi essence on memory speed.


Assuntos
Bacopa/química , Fezes/química , Metabolômica/métodos , Morus/química , Extratos Vegetais/administração & dosagem , Plasma/química , Urina/química , Idoso , Idoso de 80 Anos ou mais , Cromatografia Líquida , Método Duplo-Cego , Feminino , Sucos de Frutas e Vegetais , Voluntários Saudáveis , Humanos , Masculino , Pessoa de Meia-Idade , Extratos Vegetais/farmacocinética
4.
Int J Mol Sci ; 22(11)2021 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-34064054

RESUMO

It is now more than 90 years since Irving Langmuir used the technical term "plasma" to describe an ionized gas [...].


Assuntos
Plasma/metabolismo , Biologia/métodos , Humanos , Cicatrização/fisiologia
7.
Nat Commun ; 12(1): 2493, 2021 05 03.
Artigo em Inglês | MEDLINE | ID: mdl-33941778

RESUMO

The need for precision medicine approaches to monitor health and disease makes it important to develop sensitive and accurate assays for proteome profiles in blood. Here, we describe an approach for plasma profiling based on proximity extension assay combined with next generation sequencing. First, we analyze the variability of plasma profiles between and within healthy individuals in a longitudinal wellness study, including the influence of genetic variations on plasma levels. Second, we follow patients newly diagnosed with type 2 diabetes before and during therapeutic intervention using plasma proteome profiling. The studies show that healthy individuals have a unique and stable proteome profile and indicate that a panel of proteins could potentially be used for early diagnosis of diabetes, including stratification of patients with regards to response to metformin treatment. Although validation in larger cohorts is needed, the analysis demonstrates the usefulness of comprehensive plasma profiling for precision medicine efforts.


Assuntos
Proteínas Sanguíneas/análise , Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/diagnóstico , Plasma/química , Proteoma/análise , Idoso , Diabetes Mellitus Tipo 2/genética , Diagnóstico Precoce , Feminino , Variação Genética/genética , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Hipoglicemiantes/uso terapêutico , Estudos Longitudinais , Masculino , Metformina/uso terapêutico , Pessoa de Meia-Idade , Medicina de Precisão/métodos , Proteômica/métodos
8.
Anal Chim Acta ; 1167: 338578, 2021 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-34049630

RESUMO

Exosomes are membrane-bound, cell-secreted vesicles, with sizes ranging from 30 to 150 nm. Exosomes in blood plasma have become proposed targets as measurable indicators of disease conditions. Current methods for plasma-based exosome isolation are time-consuming, complex, and have high operational costs. One of the most commonly reported shortcomings of current isolation protocols is the co-extraction of lipoproteins (e.g. low-density lipoproteins, LDLs) with the target exosomes. This report describes the use of a rapid, single-operation hydrophobic interaction chromatography (HIC) procedure on a polyester (PET) capillary-channeled polymer (C-CP) fiber column, demonstrating the ability to efficiently purify exosomes. The method has previously been demonstrated for isolation of exosomes from diverse biological matrices, but questions were raised about the potential co-elution of LDLs. In the method described herein, a step-gradient procedure sequentially elutes spiked lipoproteins and blood plasma-originating exosomes in 10 min, with the LDLs excluded from the desired exosome fraction. Mass spectrometry (MS) was used to characterize an impurity in the primary LDL material, identifying the presence of exosomal material. Transmission electron microscopy (TEM) and an enzyme-linked immunosorbent assay (ELISA) were used to identify the various elution components. The method serves both as a rapid means of high purity exosome isolation as well as a screening tool for the purity of LDL samples with respect to extracellular vesicles.


Assuntos
Exossomos , Cromatografia , Humanos , Interações Hidrofóbicas e Hidrofílicas , Lipoproteínas LDL , Plasma , Poliésteres , Polímeros
9.
Anal Chim Acta ; 1166: 338573, 2021 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-34022993

RESUMO

Acidic lipids are associated with the regulation of the structure and function of membrane proteins. Therefore, accurate and highly precise analysis of acidic lipids is important for elucidating their biological roles and pathological mechanisms. In this study, an enhanced analytical method for the separation and quantification of acidic lipids, including phosphatidylserine (PS), phosphatidic acid (PA), cardiolipin, and their lyso-derivatives, was developed using nanoflow ultrahigh performance liquid chromatography-electrospray ionisation-tandem mass spectrometry. The separation and mass spectrometry detection of acidic lipids were optimised in terms of peak tailing and time-based separation efficiencies, with carbamate-embedded C18 as the stationary phase, in the presence of an appropriate liquid chromatography solvent modifier. This newly developed method was applied to analyse a lipid extract from porcine brain. A significant increase in the number of acidic lipids identified (176 vs. 134), including intact monolysocardiolipin (17 vs. 4), was observed with the new method compared with conventional C18. The quantification of acidic lipids was validated with plasma standard (NIST SRM 1950) spiked with a number of LPS and PS standards, and acceptable accuracy (<15%) was obtained. The present method was found to be reliable for the acidic lipid analysis based on qualitative results from tissue extract and plasma samples.


Assuntos
Plasma , Animais , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Espectrometria de Massas , Solventes , Suínos
10.
Anal Methods ; 13(17): 2021-2029, 2021 05 06.
Artigo em Inglês | MEDLINE | ID: mdl-33956002

RESUMO

In the present study, a label-free electrochemical genosensor was designed based on ZnS quantum dots functionalized with l-cysteine (Cys-ZnS-QDs) to detect miR-200a, as a special ovarian cancer biomarker. The Cys-ZnS-QD genosensor was characterized by transmission electron microscopy (TEM), UV-Vis absorption and fluorescence methods. Cys-ZnS-QDs are electrodeposited on the glassy carbon electrode surface and act as a suitable substrate for immobilization of the DNA probe. The effective parameters in the preparation of the genosensor are optimized to improve its analytical performance. The analytical performance of the genosensor has been investigated using electrochemical impedance spectroscopy. Under optimal conditions, the linear range and the detection limit of miR-200a were found to be 1.0 × 10-14 to 1.0 × 10-6 M and 8.4 fM. In addition, the genosensor is used to detect the target complementary miRNA strand from a single-base mismatch miRNA strand. Finally, this label-free electrochemical biosensor was used to detect miR-200a in human plasma without using any amplification method.


Assuntos
Técnicas Biossensoriais , MicroRNAs , Neoplasias , Pontos Quânticos , Biomarcadores Tumorais/genética , Cisteína , Humanos , MicroRNAs/genética , Plasma , Sulfetos , Compostos de Zinco
11.
Washington, D.C; Organización Panamericana de la Salud; Versión 3; Mayo, 10, 2021. 126 p. ilus.
Não convencional em Espanhol | BIGG - guias GRADE | ID: biblio-1253092

RESUMO

Esta guía de práctica clínica, en cuya elaboración se siguió el método GRADE, tiene por objeto proveer recomendaciones para el manejo de pacientes adultos críticos con COVID-19 atendidos en las unidades de cuidados intensivos. Ofrece recomendaciones basadas en evidencia para la identificación de marcadores y factores de riesgo de mortalidad de los pacientes críticos, el control de la infección, la recogida de muestras, el cuidado de los pacientes (ventilatorio y hemodinámico), el tratamiento farmacológico, la rehabilitación temprana, el uso de imágenes diagnósticas, la prevención de complicaciones y los criterios de egreso. En esta tercera versión se actualizan cinco preguntas incluidas en la primera versión y se añaden tres preguntas formuladas en formato PICO. Las recomendaciones están dirigidas a todo el personal de salud que atiende a pacientes en los servicios de urgencias y de emergencias y las unidades de cuidados intensivos. Asimismo, tienen por objetivo brindar apoyo a los responsables de la toma de decisiones y miembros de entidades gubernamentales relacionados con el manejo de pacientes con COVID-19 en las unidades de cuidados intensivos de la Región de las Américas. Esta guía se encuentra en proceso continuo de actualización de la evidencia con el fin de proporcionar las recomendaciones más actualizadas para el manejo de los pacientes críticos con COVID-19, en especial sobre las intervenciones de tratamiento farmacológico como el uso de antivirales, inmunomoduladores, plasma de convalecientes y antibióticos.


Assuntos
Humanos , Adulto , Administração dos Cuidados ao Paciente , Cuidados Críticos , SARS-CoV-2/efeitos dos fármacos , COVID-19/tratamento farmacológico , Plasma/imunologia , América/epidemiologia , Ventiladores Mecânicos , Fatores de Risco , Antirretrovirais/uso terapêutico , COVID-19/complicações , COVID-19/diagnóstico por imagem , Fatores Imunológicos/uso terapêutico , Antibacterianos/uso terapêutico
12.
Xenobiotica ; 51(6): 643-656, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33845715

RESUMO

Colchicine is widely investigated for cardioprotection of COVID-19 patients since it can prevent the phenomenon of 'cytokine storm' and may reduce the complications arising from COVID-19. Despite the potentially beneficial effects of colchicine, there is no consensus on the appropriate dosage regimen and numerous schemes are currently used.In this study, simulations were performed to identify the ability of dosage regimens to attain plasma levels in CVOID-19 patients, known to be generally safe and efficacious. Since renal and hepatic impairment, as well as, drug-drug interactions have been identified to be the most significant factors increasing colchicine toxicity, the impact of these interactions was assessed in the simulations.Some dosage regimens lead to high colchicine concentrations, while others result in sub-therapeutic levels. Additional dosage schemes were proposed in this study aiming to be applied in patients with clearance insufficiency. Colchicine administration of 0.5 mg twice daily, can be considered safe and effective. In cases of clearance impairment, doses as low as 0.25 mg thrice or twice daily should be applied.Colchicine is a narrow therapeutic index drug and dosage regimens tailored to patients' needs should be designed.


Assuntos
COVID-19 , Colchicina , Interações Medicamentosas , Humanos , Plasma , SARS-CoV-2
13.
Int J Mol Sci ; 22(9)2021 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-33926125

RESUMO

Platelet concentrates and especially their further product platelet lysate, are widely used as a replacement for cell culturing. Platelets contain a broad spectrum of growth factors and bioactive molecules that affect cellular fate. However, the cellular response to individual components of the human platelet concentrate is still unclear. The aim of this study was to observe cellular behavior according to the individual components of platelet concentrates. The bioactive molecule content was determined. The cells were supplemented with a medium containing 8% (v/v) of platelet proteins in plasma, pure platelet proteins in deionized water, and pure plasma. The results showed a higher concentration of fibrinogen, albumin, insulin growth factor I (IGF-1), keratinocyte growth factor (KGF), and hepatocyte growth factor (HGF), in the groups containing plasma. On the other hand, chemokine RANTES and platelet-derived growth factor bb (PDGF-bb), were higher in the groups containing platelet proteins. The groups containing both plasma and plasma proteins showed the most pronounced proliferation and viability of mesenchymal stem cells and fibroblasts. The platelet proteins alone were not sufficient to provide optimal cell growth and viability. A synergic effect of platelet proteins and plasma was observed. The data indicated the importance of plasma in platelet lysate for cell growth.


Assuntos
Plaquetas/química , Plaquetas/metabolismo , Plasma Rico em Plaquetas/metabolismo , Albuminas , Becaplermina/metabolismo , Técnicas de Cultura de Células/métodos , Proliferação de Células/efeitos dos fármacos , Quimiocinas/metabolismo , Meios de Cultura/química , Fibrinogênio/metabolismo , Fator 7 de Crescimento de Fibroblastos , Fibroblastos/metabolismo , Fator de Crescimento de Hepatócito , Humanos , Fator de Crescimento Insulin-Like I , Células-Tronco Mesenquimais/metabolismo , Plasma/química , Proteínas Proto-Oncogênicas c-sis/metabolismo
14.
Biochemistry (Mosc) ; 86(3): 370-381, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33838636

RESUMO

Modulation of cytokine production by physical activity is of considerable interest, since it might be a promising strategy for correcting metabolic processes at both cellular and systemic levels. The content of IL-6, IL-8, and IL-15 in the plasma and the concentration of monovalent cations in the skeletal muscles of trained and untrained mice were studied at different periods after static and dynamic exercises. Dynamic loads caused an increase in the IL-6 content and decrease in the IL-15 content in the plasma of untrained mice, but produced no effect on the concentration of IL-8. In trained mice, the effect of a single load on the concentration of IL-6 and IL-15 in the plasma was enhanced, while the concentration of IL-8 decreased. Static loads produced a similar, but more pronounced effect on the plasma concentration of IL-6 and IL-15 compared the dynamic exercises; however, the concentration of IL-8 in response to the static exercise increased significantly. Prior training reinforced the described response for all the myokines studied. Dynamic load (swimming) increased the intracellular content of sodium but decreased the content of potassium in the mouse musculus soleus. Similar response was observed after the static load (grid hanging) in the musculus biceps; but no correlation of this response with the prior training was found. Possible mechanisms involved in the regulation of cytokine secretion after exercise are discussed, including triggering of gene transcription in response to changes in the [Na+]i/[K+]I ratio.


Assuntos
Citocinas/sangue , Músculo Esquelético/fisiologia , Condicionamento Físico Animal , ATPase Trocadora de Sódio-Potássio/metabolismo , Animais , Cátions Monovalentes , Interleucina-15/sangue , Interleucina-6/sangue , Interleucina-8/sangue , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Músculo Esquelético/química , Músculo Esquelético/enzimologia , Músculo Esquelético/metabolismo , Plasma/metabolismo , Potássio/análise , Potássio/química , Sódio/análise , Sódio/química
16.
BMC Infect Dis ; 21(1): 357, 2021 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-33863281

RESUMO

BACKGROUND: In 2020, a new coronavirus, SARS-CoV-2, quickly spread worldwide within a few months. Although coronaviruses typically infect the upper or lower respiratory tract, the virus RNA can be detected in plasma. The risk of transmitting coronavirus via transfusion of blood products remains. As more asymptomatic infections are identified in COVID-19 cases, blood safety has become particularly important. Methylene blue (MB) photochemical technology has been proven to inactivate lipid-enveloped viruses with high efficiency and safety. The present study aimed to investigate the SARS-CoV-2 inactivation effects of MB in plasma. METHODS: The SARS-CoV-2 virus strain was isolated from Zhejiang University. The live virus was harvested from cultured VERO-E6 cells, and mixed with MB in plasma. The MB final concentrations were 0, 1, 2, and 4 µM. The "BX-1 AIDS treatment instrument" was used at room temperature, the illumination adjusted to 55,000 ± 0.5 million Lux, and the plasma was irradiated for 0, 2, 5, 10, 20, and 40 mins using light at a single wavelength of 630 nm. Virus load changes were measured using quantitative reverse transcription- PCR. RESULTS: BX-1 could effectively eliminate SARS-CoV-2 within 2 mins in plasma, and the virus titer declined to 4.5 log10 TCID50 (median tissue culture infectious dose)/mL. CONCLUSION: BX-1 is based on MB photochemical technology, which was designed to inactivate HIV-1 virus in plasma. It was proven to be safe and reliable in clinical trials of HIV treatment. In this study, we showed that BX-1 could also be applied to inactivate SARS-CoV-2. During the current outbreak, this technique it has great potential for ensuring the safety of blood transfusions, for plasma transfusion therapy in recovering patients, and for preparing inactivated vaccines.


Assuntos
Segurança do Sangue , COVID-19/prevenção & controle , COVID-19/terapia , Azul de Metileno/farmacologia , SARS-CoV-2/efeitos dos fármacos , Inativação de Vírus , Animais , Transfusão de Sangue , Chlorocebus aethiops , Humanos , Imunização Passiva , Plasma/virologia , RNA Viral , Células Vero
17.
Blood Adv ; 5(8): 2079-2086, 2021 04 27.
Artigo em Inglês | MEDLINE | ID: mdl-33877293

RESUMO

Data on infants and toddlers (ITs) with von Willebrand disease (VWD) are lacking. We used data collected in the US Hemophilia Treatment Center Network (USHTCN) to describe birth characteristics, bleeding episodes, and complications experienced by 105 patients with VWD who were <2 years of age. In 68% of the patients, the reason for diagnostic testing was a family history of a bleeding disorder. The mean age at diagnosis was 7 months, with little variation by sex. Patients with type 2 VWD were diagnosed earlier than those with types 1 or 3 (P = .04), and those with a family history were diagnosed ∼4 months earlier than those with none (P < .001). Among the patients who experienced a bleeding event (70%), oral mucosa was the most common site of the initial bleeding episode (32%), followed by circumcision-related (12%) and intracranial/extracranial bleeding (10%). Forty-one percent of the initial bleeding events occurred before 6 months of age, and 68% of them occurred before the age of 1 year. Approximately 5% of the cohort experienced an intracranial hemorrhage; however, none was associated with delivery at birth. Bleeding patterns and rates were similar by sex (P = .40) and VWD type (P = .10). Forty-seven percent were treated with plasma-derived von Willebrand factor VIII concentrates. The results of this study indicate that a high percentage of ITs diagnosed with VWD and receiving care within the multidisciplinary structure of the USHTCN have a family history of VWD. In addition, bleeding events such as circumcision-related, oropharyngeal, and intracranial or extracranial episodes are common and are leading indicators for treatment.


Assuntos
Doenças de von Willebrand , Idoso , Pré-Escolar , Estudos de Coortes , Hemorragia/epidemiologia , Hemorragia/etiologia , Humanos , Lactente , Recém-Nascido , Masculino , Plasma , Doenças de von Willebrand/diagnóstico , Doenças de von Willebrand/epidemiologia , Doenças de von Willebrand/etiologia , Fator de von Willebrand
18.
Molecules ; 26(6)2021 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-33799523

RESUMO

Parabens have been widely employed as preservatives since the 1920s for extending the shelf life of foodstuffs, medicines, and daily care products. Given the fact that there are some legitimate concerns related to their potential multiple endocrine-disrupting properties, the development of novel bioanalytical methods for their biomonitoring is crucial. In this study, a fabric phase sorptive extraction reversed-phase liquid chromatography method coupled with UV detection (FPSE-HPLC-UV) was developed and validated for the quantitation of seven parabens in human plasma samples. Chromatographic separation of the seven parabens and p-hydroxybenzoic acid was achieved on a semi-micro Spherisorb ODS1 analytical column under isocratic elution using a mobile phase containing 0.1% (v/v) formic acid and 66% 49 mM ammonium formate aqueous solution in acetonitrile at flow rate 0.25 mL min-1 with a 24-min run time for each sample. The method was linear at a concentration range of 20 to 500 ng mL-1 for the seven parabens under study in human plasma samples. The efficiency of the method was proven with the analysis of 20 human plasma samples collected from women subjected to breast cancer surgery and to reconstructive and aesthetic breast surgery. The highest quantitation rates in human plasma samples from cancerous cases were found for methylparaben and isobutylparaben with average plasma concentrations at 77 and 112.5 ng mL-1. The high concentration levels detected agree with previous findings for some of the parabens and emphasize the need for further epidemiological research on the possible health effects of the use of these compounds.


Assuntos
Cromatografia de Fase Reversa/métodos , Parabenos/análise , Plasma/química , Cromatografia Líquida de Alta Pressão/métodos , Disruptores Endócrinos/análise , Feminino , Humanos , Limite de Detecção , Conservantes Farmacêuticos/análise , Extração em Fase Sólida/métodos , Têxteis/análise
19.
J Vet Diagn Invest ; 33(3): 506-515, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33847203

RESUMO

Vitamin E deficiencies can impact normal growth and development in humans and animals, and assessment of circulating levels of vitamin E and its metabolites may be an important endpoint for evaluation. Development of a sensitive method to detect and quantify low concentrations of vitamin E and metabolites in biological specimens allows for a proper diagnosis for patients and animals that are deficient. We developed a method to simultaneously extract, detect, and quantify the vitamin E compounds alpha-tocopherol (α-TP), gamma-tocopherol (γ-TP), alpha-tocotrienol (α-TT), and gamma-tocotrienol (γ-TT), and the corresponding metabolites formed after ß-oxidation of α-TP and γ-TP, alpha-carboxymethylbutyl hydroxychroman (α-CMBHC) and alpha- or gamma-carboxyethyl hydroxychroman (α- or γ-CEHC), respectively, from equine plasma and serum. Quantification was achieved through liquid chromatography-tandem mass spectrometry. We applied a 96-well high-throughput format using a Phenomenex Phree plate to analyze plasma and serum. Compounds were separated by using a Waters ACQUITY UPLC BEH C18 column with a reverse-phase gradient. The limits of detection for the metabolites and vitamin E compounds were 8-330 pg/mL. To validate the method, intra-day and inter-day accuracy and precision were evaluated along with limits of detection and quantification. The method was then applied to determine concentrations of these analytes in plasma and serum of horses. Alpha-TP levels were 3-6 µg/mL of matrix; the metabolites were found at much lower levels, 0.2-1.0 ng/mL of matrix.


Assuntos
Cavalos/metabolismo , Vitamina E/sangue , Animais , Cromatografia Líquida , Feminino , Masculino , Plasma/química , Soro/química , Espectrometria de Massas em Tandem , Vitamina E/metabolismo
20.
Nutrients ; 13(3)2021 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-33801409

RESUMO

SCOPE: B vitamers are co-enzymes involved in key physiological processes including energy production, one-carbon, and macronutrient metabolism. Studies profiling B vitamers simultaneously in parent-child dyads are scarce. Profiling B vitamers in parent-child dyads enables an insightful determination of gene-environment contributions to their circulating concentrations. We aimed to characterise: (a) parent-child dyad concordance, (b) generation (children versus adults), (c) age (within the adult subgroup (age range 28-71 years)) and (d) sex differences in plasma B vitamer concentrations in the CheckPoint study of Australian children. METHODS AND RESULTS: 1166 children (11 ± 0.5 years, 51% female) and 1324 parents (44 ± 5.1 years, 87% female) took part in a biomedical assessment of a population-derived longitudinal cohort study: The Growing Up in Australia's Child Health CheckPoint. B vitamer levels were quantified by UHPLC/MS-MS. B vitamer levels were weakly concordant between parent-child pairs (10-31% of variability explained). All B vitamer concentrations exhibited generation-specificity, except for flavin mononucleotide (FMN). The levels of thiamine, pantothenic acid, and 4-pyridoxic acid were higher in male children, and those of pantothenic acid were higher in male adults compared to their female counterparts. CONCLUSION: Family, age, and sex contribute to variations in the concentrations of plasma B vitamers in Australian children and adults.


Assuntos
Deficiência de Vitamina B 6/epidemiologia , Vitamina B 6/sangue , Adulto , Idoso , Austrália , Criança , Saúde da Criança , Ingestão de Alimentos , Feminino , Humanos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Relações Pais-Filho , Pais , Plasma , Espectrometria de Massas em Tandem
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