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1.
Food Chem ; 333: 127432, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-32659661

RESUMO

The sensory qualities and shelf life of tea beverage strongly affected by tea cream that forms by the interaction of polyphenols and protein. The study aimed to investigate the effects of the interactions between tea polyphenols (TPs) and bovine serum albumin (BSA) on tea cream formation at different concentrations. The tea cream formation increased with TPs and BSA concentration increased. The optimal concentration (TPs: 800 mg/L, BSA: 40 mg/L), for high clarities and contents of phytochemicals, was selected by the technique for order preference by similarity to ideal solution (C = 0.7572). The interaction mechanism of TPs-BSA was investigated by fluorescence spectroscopy, UV-visible absorption spectroscopy, synchronous fluorescence spectroscopy, and molecular docking. TPs interacted with BSA via static quenching process, affecting tryptophan and tyrosine residue microenvironment of BSA. Ester catechins had more binding affinity than non-ester catechins. Hydrogen bonds were the main interaction forces of TPs-BSA.


Assuntos
Polifenóis/química , Polifenóis/metabolismo , Soroalbumina Bovina/química , Chá/química , Animais , Sítios de Ligação , Catequina/química , Catequina/metabolismo , Precipitação Química , Ligação de Hidrogênio , Simulação de Acoplamento Molecular , Tamanho da Partícula , Soroalbumina Bovina/metabolismo , Espectrometria de Fluorescência , Espectrofotometria Ultravioleta , Chá/metabolismo , Termodinâmica
2.
Food Chem ; 333: 127433, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-32659662

RESUMO

This study examined the effects of exogenous citrulline (control, 1 and 2 mM) and water availability (100%, 50% and 25% WA) on antioxidant attributes and essential oil constituents of Hyssopus officinalis L. in two successive harvests. Hyssop tolerantly responded to water deficiency by well-promoted antioxidant enzymes (i.e., superoxide dismutase, and catalase), strong DPPH-scavenging activity, and increasing polyphenols; however, the essential oil content was negatively reduced by water stress. External citrulline further increased the activity of antioxidant enzymes. Citrulline application at 2 mM under severe water stress could also improve essential oil (EO) content in the first and second harvests by about 15 and 30%, respectively. Furthermore, under severe drought, citrulline at 2 mM could obtain the highest yield of isopinocamphone (47%) as the main component of EO. The results showed the high potential of this novel applied metabolite agent to be used in a well-fulfilled production of this medicinal plant.


Assuntos
Antioxidantes/metabolismo , Citrulina/farmacologia , Hyssopus/efeitos dos fármacos , Hyssopus/fisiologia , Óleos Voláteis/metabolismo , Canfanos/metabolismo , Catalase/metabolismo , Catecol Oxidase/metabolismo , Desidratação , Secas , Hyssopus/química , Óleos Voláteis/análise , Proteínas de Plantas/metabolismo , Plantas Medicinais/efeitos dos fármacos , Plantas Medicinais/fisiologia , Polifenóis/metabolismo , Superóxido Dismutase/metabolismo
3.
Arch Biochem Biophys ; 690: 108471, 2020 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-32622788

RESUMO

Stilbenes, an active substances closely related to resistance and quality of grapes, are rarely found in natural resources. However its cumulative amount is affected by ultraviolet radiation (UV). The purpose of this study is to screen key genes in biosynthesis of stilbenes Trans-scripusin A and explore its synthetic pathway. We tested content of stilbenes with UHPLC-QQQ-MS2, results revealed that stilbenes accumulation is positively correlated with UV-B exposure time. Then, we performed transcriptome high-throughput sequencing of grapes under treatments. Results shown that 13,906 differentially expressed genes were obtained, which were mainly enriched in three major regions (ribosome, plant-pathogen interaction and biosynthesis of flavonoid). Three genes of trans-scripusin A synthesis pathway key got by combining KEGG annotation and reference gene HsCYP1B1. Phylogenetic analysis showed that SAH genes had high homology with other hydroxylase genes, and distributed in two subgroups. Gene structure analysis showed that SAH genes contained four exons, indicating that gene has low genetic diversity. Chromosome localization revealed that SAH genes were distributed on different chromosomes, in addition, the number of gene pairs between Vitis vinifera and other species was not related to genome size of other species. The expression profiles of SAH genes in different parts of Vitis vinifera L. were analyzed using qRT-PCR analysis, results indicated that expression of SAH genes be specific to fruit part. These paper provide theoretical basis for further study of polyphenols biosynthesis pathway in grape fruits. The study provides novel insights for further understanding quality of grapes response to UV radiation.


Assuntos
Frutas/genética , Regulação da Expressão Gênica de Plantas/efeitos da radiação , RNA Mensageiro/efeitos da radiação , Vitis/genética , Vias Biossintéticas , Cromatografia Líquida de Alta Pressão , Flavonoides/metabolismo , Frutas/metabolismo , Frutas/efeitos da radiação , Ensaios de Triagem em Larga Escala , Conformação de Ácido Nucleico , Filogenia , Polifenóis/metabolismo , RNA-Seq , Ribossomos/metabolismo , Estilbenos/metabolismo , Estresse Fisiológico/genética , Estresse Fisiológico/efeitos da radiação , Espectrometria de Massas em Tandem , Transcriptoma/efeitos da radiação , Raios Ultravioleta , Vitis/metabolismo , Vitis/efeitos da radiação
4.
Food Chem ; 329: 127219, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-32516714

RESUMO

Plant-based proteins and polyphenols are increasingly being explored as functional food ingredients. Colloidal complexes were prepared from pea protein (PP) and grape seed proanthocyanidin (GSP) and the ability of the PP/GSP complexes to form and stabilize oil-in-water emulsions were investigated. The main interactions between PP and GSP were hydrogen bonding. The stability of PP-GSP complexes to environmental changes were studied: pH (2-9); ion strength (0-0.3 M); and temperature (30-90 °C). Emulsions produced using PP-GSP complexes as emulsifiers had small mean droplet diameters (~200 nm) and strongly negative surface potentials (~-60 mV). Compared to PP alone, PP-GSP complexes slightly decreased the isoelectric point, thermostability, and salt stability of the emulsions, but increased their storage stability. The presence of GSP gave the emulsions a strong salmon (red-yellow) color, which may be beneficial for some specific applications. These results may assist in the creation of more efficacious food-based strategies for delivering proanthocyanidins.


Assuntos
Emulsões/química , Extrato de Sementes de Uva/química , Proteínas de Ervilha/química , Ervilhas/metabolismo , Polifenóis/química , Proantocianidinas/química , Antioxidantes/química , Sítios de Ligação , Calorimetria , Extrato de Sementes de Uva/metabolismo , Ligação de Hidrogênio , Concentração de Íons de Hidrogênio , Simulação de Acoplamento Molecular , Óleos/química , Concentração Osmolar , Proteínas de Ervilha/metabolismo , Polifenóis/metabolismo , Proantocianidinas/metabolismo , Cloreto de Sódio/química , Temperatura , Água/química
5.
Food Chem ; 327: 126992, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-32447133

RESUMO

The influence mechanism of different withering methods (CK, indoor natural spreading; LTD, low-temperature plus dark; LTY, low-temperature plus yellow-light; LTCD, low-temperature plus CO2) on non-volatile compounds in postharvest tea leaves was investigated by UHPLC-Q-TOF/MS-based non-targeted metabolomic and transcriptomic analyses. Compared with CK, low-temperature withering could slow down polyphenol oxidation by inhibiting polyphenol oxidase activity and keeping the expression of genes for flavanol synthesis. After withering, the proteinaceous amino acid content increased significantly, especially for LTCD and LTY, mainly due to increased peptidase activity and up-regulation of genes involved in the biosynthesis of valine, leucine, aspartic acid, glutamic acid, phenylalanine, and proline. Moreover, LTCD and LTY enhanced the synthesis of γ-aminobutyric acid and metabolism of phenylalanine-methyl salicylate and tryptophan-indole, respectively. Meanwhile, the transformation of theobromine to caffeine was accelerated under low-temperature withering. This research provides ageneticmetabolicbasis for the application of low-temperature withering to actual green tea processing.


Assuntos
Camellia sinensis/metabolismo , Chá/metabolismo , Cafeína/metabolismo , Camellia sinensis/genética , Cor , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Metabolômica , Folhas de Planta/genética , Folhas de Planta/metabolismo , Polifenóis/metabolismo , Ácido Salicílico/metabolismo , Chá/genética
6.
Food Chem ; 319: 126562, 2020 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-32155536

RESUMO

This study investigated the effects of the addition of onion waste fractions into gluten-free (GF) bread to promote its health benefits. 5% of the control (C) GF flour blend was replaced with three waste fractions in the form of: fried onion (FO), dried onion (DO) and onion peel (OP). Antioxidant activity, content of flavonols and total polyphenols of breads increased in the following order: C < FO < DO < OP. No differences were observed in sensory analysis. We found that quercetin glycosides, dimers and trimer in OP-bread, determined according to their mass spectra, decomposed during baking and released free quercetin, which points to their thermal instability. Cross-over study revealed that consumption of OP-bread significantly increased (p < 0.05) antioxidant activity of consumers' blood compared to control bread consumption, indicating good bioavailability of flavonols. Results suggest incorporation of OP into GF bread can increase its biological value with satisfactory sensory acceptance.


Assuntos
Pão/análise , Cebolas/química , Cebolas/metabolismo , Disponibilidade Biológica , Culinária , Estudos Cross-Over , Polifenóis/química , Polifenóis/metabolismo , Quercetina/química , Quercetina/metabolismo , Rutina/química , Rutina/metabolismo
7.
Environ Pollut ; 261: 114230, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32220755

RESUMO

Polyphenols, pivotal secondary metabolites, are involved in plant adaption to abiotic stresses. Here, we investigated the role and metabolism profile of polyphenols under aluminum (Al) stress in different lettuce genotypes grown in 0.5 mM CaCl2 solution with AlCl3 (pH = 4.5). The complementary use of high-resolution mass spectrometry and quantitative biochemical approaches allowed the characterization of total and unique phenols, as well as their roles in Al tolerance. By comparing the most tolerant and sensitive genotype, 8 polyphenols, including 4 phenolic acids, 2 flavonoids, 1 xanthone and 1 unknown compound, were identified in the roots of the tolerant genotype. The total phenolic and flavonoid contents significantly increased in the tolerant genotype under Al stress. Seedlings with more phenolic accumulation usually performed greater Al tolerance. Meanwhile, principal enzymes related to phenolic biosynthesis significantly increased in roots of the tolerance genotype after Al treatment, with phenylalanine ammonia lyase (PAL), cinnamate 4-hydroxylase, and 4-coumarate coenzyme A ligase increased by 16, 18 and 30%, respectively. The elevated total phenolics were significantly suppressed by AIP, a highly specific PAL inhibitor. Consequently, the antioxidant capacity was inhibited, leading to lettuce sensitivity to Al stress. These results clearly suggested the enhancement of unique polyphenolic biosynthesis as an adaptive strategy of lettuce to Al stress by protecting plants from oxidative stress.


Assuntos
Alumínio , Alface , Polifenóis , Estresse Fisiológico , Alumínio/toxicidade , Alface/efeitos dos fármacos , Raízes de Plantas/metabolismo , Polifenóis/metabolismo , Poluentes do Solo/toxicidade , Estresse Fisiológico/fisiologia
8.
Food Chem ; 317: 126415, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32087518

RESUMO

This paper focused on improving antityrosinase ability of quercetin, cinnamic acid, and ferulic acid (named Q-CA-FA) from Asparagus by combining heating with ultrasound treatments. Fluorescence spectroscopy and UPLC-MS were used to evaluate inhibitory mechanisms. Results showed that the impacts of combining heating (150 °C for 30 min) with ultrasound (600 W for 30 min) treatments were similar to heating treatment (150 °C for 120 min) alone, and the inhibition rate could reach 98.2% in the addition of 5 mM Q-CA-FA. Fluorescence quenching indicated that treated Q-CA-FA-tyrosinase complex was more stable, but combining treatments did not change the major force between tyrosinase and polyphenols. Thermodynamic analysis illustrated that the randomness of compounds was also increased. Interestingly, 2-hydroxy-3-(3-hydroxy-4-methoxy-phenyl)-propionic acid 4-(2,3-dihydroxy-propyl)-phenyl ester was newly detected, which might be the major reason for enhancing antityrosinase ability. Taken together, these results provide a creative insight on increasing antityrosinase activity by combining heating with ultrasound treatments.


Assuntos
Monofenol Mono-Oxigenase/metabolismo , Polifenóis/metabolismo , Sonicação , Asparagus (Planta)/química , Asparagus (Planta)/metabolismo , Cromatografia Líquida de Alta Pressão , Cinamatos/análise , Cinamatos/metabolismo , Ácidos Cumáricos/análise , Ácidos Cumáricos/metabolismo , Temperatura Alta , Monofenol Mono-Oxigenase/antagonistas & inibidores , Polifenóis/análise , Quercetina/análise , Quercetina/metabolismo , Espectrometria de Fluorescência , Espectrometria de Massas em Tandem , Termodinâmica
9.
Food Chem ; 317: 126423, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32097824

RESUMO

The impact of pH (6-9) and NaCl concentration (0-0.5 mol.L-1) on sunflower protein extraction was studied through design of experiments. The considered criteria were protein extraction yield (total proteins, helianthinin and albumins), chlorogenic acids covalently bound to proteins, and free chlorogenic acid concentration in the aqueous extract. Statistical analysis showed that the obtained by design of experiments the polynomial models of each extraction criteria were reliable for predicting the responses. They were employed in an original multi-objective optimization methodology. The optimal conditions revealed to be pH 7.3/0.3 mol.L-1 NaCl yielded 46.83% and 59.16% of total protein and albumin extraction yield, 1.730 and 1.998 mg.g-1 of chlorogenic acids covalently bound to helianthinin and albumins in aqueous extract, respectively. The sunflower protein isolate obtained after extraction in this condition had good solubility (40-80% at pH 5-8), functional properties (foaming and emulsifying) and a satisfying color.


Assuntos
Helianthus/metabolismo , Extração Líquido-Líquido/métodos , Proteínas de Plantas/isolamento & purificação , Extração em Fase Sólida/métodos , Albuminas/análise , Albuminas/isolamento & purificação , Albuminas/metabolismo , Ácido Clorogênico/química , Ácido Clorogênico/metabolismo , Concentração de Íons de Hidrogênio , Isomerismo , Extração Líquido-Líquido/instrumentação , Proteínas de Plantas/análise , Proteínas de Plantas/metabolismo , Polifenóis/análise , Polifenóis/metabolismo , Ligação Proteica , Cloreto de Sódio/química , Extração em Fase Sólida/instrumentação
10.
Phys Chem Chem Phys ; 22(4): 2212-2228, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31913367

RESUMO

In this work, the interaction of a bioactive tea polyphenol (-)-epigallocatechin gallate (EGCG) with bovine hemoglobin (BHb) along with its anti-oxidative behavior and the anti-glycation property have been explored using multi-spectroscopic and computational techniques. The binding affinity for EGCG towards BHb was observed to be moderate in nature with an order of 104 M-1, and the fluorescence quenching mechanism was characterized by an unusual static quenching mechanism. The binding constant (Kb) showed a continuous enhancement with temperature from 3.468 ± 0.380 × 104 M-1 at 288 K to 6.017 ± 0.601 × 104 M-1 at 310 K. The fluorescence emission measurements along with molecular docking studies indicated that EGCG binds near the most dominant fluorophore of BHb (ß2-Trp37, at the interface of α1 and ß2 chains) within the pocket formed by the α1, α2 and ß2 chains. The sign and magnitude of the thermodynamic parameters, changes in enthalpy (ΔH = +17.004 ± 1.007 kJ mol-1) and in entropy (ΔS = +146.213 ± 2.390 J K-1 mol-1), indicate that hydrophobic forces play a major role in stabilizing the BHb-EGCG complex. The micro-environment around the EGCG binding site showed an increase in hydrophobicity upon ligand binding. The binding of EGCG with BHb leads to a decrease in the α-helical content, whereas that of the ß-sheet increased. FTIR studies also indicated that the secondary structure of BHb changed upon binding with EGCG, along with providing further support for the presence of hydrophobic forces in the complexation process. Molecular docking studies indicated that EGCG binds within the cavity of α1, α2, and ß2 chains surrounded by residues such as α1- Lys99, α1-Thr134, α1-Thr137, α1-Tyr140, α2-Lys127 and ß2-Trp37. Molecular dynamics simulation studies indicated that EGCG conferred additional stability to BHb. Furthermore, moving away from the binding studies, EGCG was found to prevent the glyoxal (GO)-mediated glycation process of BHb, and it was also found to act as a potent antioxidant against the photo-oxidative damage of BHb.


Assuntos
Catequina/análogos & derivados , Hemoglobinas/química , Hemoglobinas/metabolismo , Polifenóis/metabolismo , Animais , Catequina/química , Bovinos , Interações Hidrofóbicas e Hidrofílicas , Simulação de Acoplamento Molecular , Polifenóis/química , Ligação Proteica , Análise Espectral
11.
PLoS One ; 15(1): e0228157, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31978155

RESUMO

Insect herbivores have the potential to change both physical and chemical traits of their host plant. Although the impacts of herbivores on their hosts have been widely studied, experiments assessing changes in multiple leaf traits or functions simultaneously are still rare. We experimentally tested whether herbivory by winter moth (Operophtera brumata) caterpillars and mechanical leaf wounding changed leaf mass per area, leaf area, leaf carbon and nitrogen content, and the concentrations of 27 polyphenol compounds on oak (Quercus robur) leaves. To investigate how potential changes in the studied traits affect leaf functioning, we related the traits to the rates of leaf photosynthesis and respiration. Overall, we did not detect any clear effects of herbivory or mechanical leaf damage on the chemical or physical leaf traits, despite clear effect of herbivory on photosynthesis. Rather, the trait variation was primarily driven by variation between individual trees. Only leaf nitrogen content and a subset of the studied polyphenol compounds correlated with photosynthesis and leaf respiration. Our results suggest that in our study system, abiotic conditions related to the growth location, variation between tree individuals, and seasonal trends in plant physiology are more important than herbivory in determining the distribution and composition of leaf chemical and structural traits.


Assuntos
Mariposas/fisiologia , Quercus/química , Animais , Carbono/metabolismo , Herbivoria , Interações Hospedeiro-Parasita , Larva/fisiologia , Mariposas/crescimento & desenvolvimento , Nitrogênio/metabolismo , Fotossíntese , Folhas de Planta/química , Folhas de Planta/metabolismo , Folhas de Planta/parasitologia , Brotos de Planta/química , Brotos de Planta/metabolismo , Polifenóis/metabolismo , Análise de Componente Principal , Quercus/metabolismo , Quercus/parasitologia , Estações do Ano , Estresse Mecânico
12.
J Agric Food Chem ; 68(2): 612-622, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31903750

RESUMO

Corn (Zea mays L.) growth and development is often limited by the availability of phosphate. We thus hypothesized that phosphate fertilization may increase the contents of (poly)phenols, carotenoids, and tocochromanols (vitamin E) in corn grains. Corn plants cultivated on a soil fertilized with 44 kg phosphorus/ha were compared to plants grown on soil with low plant-available phosphate (1.6 mg CAL-P/100 g of soil), each sown early (April) and late (May) in a randomized field experiment. HPLC-DAD-(HR)-ESI-MSn revealed 19 soluble and 10 insoluble (poly)phenols, comprising phenolic acids, phenolic amines, diferulic, and triferulic acids in corn grains. Contents of individual (poly)phenols, carotenoids, and tocochromanols in whole grains were significantly (p < 0.05) increased by sowing time, but not by phosphate fertilization. In conclusion, low phosphate availability did not impair the biosynthesis of (poly)phenols, carotenoids, and tocochromanols in corn grains.


Assuntos
Carotenoides/análise , Fenóis/análise , Fosfatos/metabolismo , Polifenóis/análise , Sementes/química , Vitamina E/análise , Zea mays/crescimento & desenvolvimento , Carotenoides/metabolismo , Fertilizantes/análise , Fenóis/metabolismo , Polifenóis/metabolismo , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Vitamina E/metabolismo , Zea mays/química , Zea mays/metabolismo
13.
J Agric Food Chem ; 68(5): 1315-1325, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31913632

RESUMO

This study examined the bioaccessibility of polyphenolic compounds originating directly from fruits or from fruit extracts during their digestion conducted in a simulated human digestive tract. The results demonstrated that polyphenols bound to the food matrix are less bioavailable, but the type of food matrix plays an important role. Depending on the raw material, 14 to 58% of polyphenols present in fruit extracts were transferred to the supernatant, while in the case of polyphenols present in fruits, only 5-9% were transferred. Sediments obtained after in vitro digestion at the stomach and duodenum stage of fruit extracts contained virtually no polyphenols and demonstrated negligible antioxidant activity, whereas after digestion of whole fruits, the detected polyphenols constituted 5-44%. The intestinal microbiota were actively involved in the metabolism of polyphenols, mainly anthocyanins and glycosides remaining after the earlier stages of digestion.


Assuntos
Frutas/metabolismo , Mucosa Gástrica/metabolismo , Extratos Vegetais/metabolismo , Polifenóis/metabolismo , Disponibilidade Biológica , Digestão , Frutas/química , Mucosa Gástrica/química , Humanos , Modelos Biológicos , Extratos Vegetais/química , Polifenóis/química
14.
Food Chem ; 312: 126077, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-31891885

RESUMO

Polyphenols from five pigmented sorghum (PS) flours were in vitro evaluated as possible modulators of starch digestibility. White sorghum (WS) flour was used as control. Untargeted metabolomics depicted the phenolic composition of raw and cooked flours (obtained through heating at 100 °C for 30 min in water) highlighting differences in flavonoids and phenolic acids. Raw PS flours were characterized by greater tannin and kafirin contents when compared to WS, and, after cooking, PS flours had greater resistant starch (from 4.2 to 21.4 g /100 g dry matter), and lower starch hydrolysis index (HI) with respect to cooked WS. Multivariate statistics showed that flavonoids characterizing PS were the most discriminant compounds during the in vitro digestion. In addition, kafirin and total tannins content (on raw ingredients) along with the anthocyanin profiles (on cooked samples) were negative correlated with HI. Therefore, PS flours might be good candidates for the formulation of functional foods.


Assuntos
Polifenóis/química , Sorghum/química , Amido/química , Culinária , Grão Comestível/química , Grão Comestível/metabolismo , Farinha/análise , Hidrólise , Metabolômica , Polifenóis/metabolismo , Sorghum/metabolismo , Amido/metabolismo , Taninos
16.
J Agric Food Chem ; 68(7): 1790-1807, 2020 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-31241945

RESUMO

Age-associated pathophysiological changes such as neurodegenerative diseases are multifactorial conditions with increasing incidence and no existing cure. The possibility of altering the progression and development of these multifactorial diseases through diet is an attractive approach with increasing supporting data. Epidemiological and clinical studies have highlighted the health potential of diets rich in fruits and vegetables. Such food sources are rich in (poly)phenols, natural compounds increasingly associated with health benefits, having the potential to prevent or retard the development of various diseases. However, absorption and the blood concentration of (poly)phenols is very low when compared with their corresponding (poly)phenolic metabolites. Therefore, these serum-bioavailable metabolites are much more promising candidates to overcome cellular barriers and reach target tissues, such as the brain. Bearing this in mind, it will be reviewed that the molecular mechanisms underlying (poly)phenolic metabolites effects, range from 0.1 to <50 µM and their role on neuroinflammation, a central hallmark in neurodegenerative diseases.


Assuntos
Doenças Neurodegenerativas/dietoterapia , Doenças Neurodegenerativas/imunologia , Extratos Vegetais/metabolismo , Polifenóis/metabolismo , Animais , Frutas/química , Frutas/metabolismo , Humanos , Peso Molecular , Doenças Neurodegenerativas/genética , Doenças Neurodegenerativas/metabolismo , Extratos Vegetais/química , Polifenóis/química , Verduras/química , Verduras/metabolismo
17.
Prep Biochem Biotechnol ; 50(2): 123-132, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31702433

RESUMO

The chemo-profiling of ethanolic extract of faba beans seeds was performed and explored as an α-glucosidase inhibitor. The inhibition of α-glucosidase is one of the alternatives approach to control postprandial hyperglycemia by, resulting in the delay of the carbohydrate digestion of absorbable monosaccharides. Ethanolic seed extract showed phenolic compounds, flavonoid such as gallic acid (m/z [M- H] = 169.0124,C7H6O5) ellagic acid derivatives epigallocatechin (m/z [M- H = 305.0644,C15H14O7),catechin (m/z [M- H] = 289.0656,C15H14O6), epigallocatechin gallate (m/z [M- H] = 457.0578,C22H18O11) and epicatechin monogallate (m/z [M- H] = 441.081, C22H18O10). The extract was found to exert inhibitory activity (88.28 ± 2.67%) (IC50 value of 2.30 ± 0.032 mg/mL) with a mixed mode of inhibition (Km, apparent = 0.54 ± 0.020 mM and Vmax, apparent 0.136 ± 0.04 mM/min). Molecular docking studies of gallic acid and catechin on α-glucosidase proposed productive binding modes having binding energy (-6.58 kcal/mol and -7.25 kcal/mol) with an effective number of hydrogen bonds and binding energy. Tyr63, Arg197, Asp198, Glu 233, Asn324, Asp 326 of α-glucosidase participated in binding events with gallic acid and catechin. Molecular dynamics simulation studies were performed for both complexes i.e. gal:α-glucosidase and cat:α-glucosidase along with apo state of α-glucosidase, which revealed stable systems during the simulation. These findings of the present study may give an insight into the further development of the novel antidiabetic drug from the seeds of faba beans.


Assuntos
Catequina/metabolismo , Ácido Gálico/metabolismo , Extratos Vegetais/farmacologia , Polifenóis/metabolismo , Vicia faba/metabolismo , alfa-Glucosidases/metabolismo , Cromatografia Líquida de Alta Pressão , Inibidores de Glicosídeo Hidrolases/farmacologia , Simulação de Acoplamento Molecular , Sementes/química , Espectrometria de Massas por Ionização por Electrospray , Vicia faba/embriologia
18.
J Agric Food Chem ; 68(3): 769-778, 2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-31826608

RESUMO

Previous work with Caco-2 cell cultures has shown that individual polyphenols can either promote or inhibit iron uptake. This investigation was designed to characterize the relationship between iron bioavailability and seed coat polyphenol composition in a panel of 14 yellow beans representing five market classes with the potential for fast cooking time and high iron content. The study included two white and two red mottled bean lines, which represent high and low iron bioavailability capacity in dry beans, respectively. Polyphenols were measured quantitatively by high-performance liquid chromatography-mass spectrometry (HPLC-MS)/UV and iron bioavailability of seed coat extracts was measured in Caco-2 assays. Thirteen of the yellow bean seed types contained high concentrations (up to 35.3 ± 2.7 µmol/g) of kaempferol 3-glucoside (k 3-g), a known promoter of iron uptake. A general association between the ratio of promoting to inhibiting polyphenols (P/I) and iron uptake was observed. The presence of iron uptake inhibiting condensed tannins proportionately countered the promotional effects of kaempferol compounds. Unidentified factors present in seed coats other than polyphenols also appeared to affect iron uptake.


Assuntos
Ferro/metabolismo , Phaseolus/química , Extratos Vegetais/química , Polifenóis/química , Disponibilidade Biológica , Transporte Biológico , Células CACO-2 , Humanos , Ferro/química , Phaseolus/classificação , Phaseolus/metabolismo , Extratos Vegetais/metabolismo , Polifenóis/metabolismo , Sementes/química , Sementes/metabolismo
19.
J Agric Food Chem ; 68(2): 574-583, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31820633

RESUMO

Food structure is a key determinant for the release of phenolic compounds during gastric and intestinal digestion. We evaluated the bioaccessibility of polyphenols from apple tissue during gastric digestion in vitro from bio-mechanical perspectives including the effects of gastric juice and mucin on the apple tissue matrix under simulated stomach peristalsis. The gastric model system was effective in releasing polyphenols because of simultaneous compression and extrusion, with 3 times higher release from coarse than from fine particles. However, bioaccessibility of polyphenols was reduced up to 44% in the presence of both cell walls and gastric mucin. Most individual phenolic molecules were gradually released and were stable in the gastric environment, except for procyanidin B2. The study suggests that the bioaccessibility of polyphenols from apples in the upper digestive tract is dependent on mechanical disintegration and the residual matrix present in the swallowed bolus.


Assuntos
Digestão , Malus/metabolismo , Polifenóis/metabolismo , Estômago/fisiologia , Disponibilidade Biológica , Parede Celular/química , Parede Celular/metabolismo , Frutas/química , Frutas/metabolismo , Humanos , Malus/química , Modelos Biológicos , Polifenóis/química
20.
J Agric Food Chem ; 68(1): 33-47, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31829012

RESUMO

Obesity is caused by an imbalance of energy intake and expenditure. It is characterized by a higher accumulation of body fat with a chronic low-grade inflammation. Many reports have shown that gut microbiota in the host plays a pivotal role in mediating the interaction between consumption of a high-fat diet (HFD) and onset of obesity. Accumulative evidence has suggested that the changes in the composition of gut microbiota may affect the host's energy homeostasis, systemic inflammation, lipid metabolism, and insulin sensitivity. As one of the major components in human diet, polyphenols have demonstrated to be capable of modulating the composition of gut microbiota and reducing the HFD-induced obesity. The present review summarizes the findings of recent studies on dietary polyphenols regarding their metabolism and interaction with bacteria in the intestine as well as the underlying mechanisms by which they modulate the gut microbiota and alleviate the HFD-induced obesity.


Assuntos
Dieta Hiperlipídica/efeitos adversos , Microbioma Gastrointestinal , Obesidade/dietoterapia , Obesidade/microbiologia , Polifenóis/metabolismo , Animais , Ingestão de Energia , Humanos , Obesidade/metabolismo
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