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1.
Signal Transduct Target Ther ; 5(1): 220, 2020 10 06.
Artigo em Inglês | MEDLINE | ID: mdl-33024075
2.
Molecules ; 25(18)2020 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-32899754

RESUMO

The emergence of the Coronavirus Disease 2019 (COVID-19) caused by the SARS-CoV-2 virus has led to an unprecedented pandemic, which demands urgent development of antiviral drugs and antibodies; as well as prophylactic approaches, namely vaccines. Algae biotechnology has much to offer in this scenario given the diversity of such organisms, which are a valuable source of antiviral and anti-inflammatory compounds that can also be used to produce vaccines and antibodies. Antivirals with possible activity against SARS-CoV-2 are summarized, based on previously reported activity against Coronaviruses or other enveloped or respiratory viruses. Moreover, the potential of algae-derived anti-inflammatory compounds to treat severe cases of COVID-19 is contemplated. The scenario of producing biopharmaceuticals in recombinant algae is presented and the cases of algae-made vaccines targeting viral diseases is highlighted as valuable references for the development of anti-SARS-CoV-2 vaccines. Successful cases in the production of functional antibodies are described. Perspectives on how specific algae species and genetic engineering techniques can be applied for the production of anti-viral compounds antibodies and vaccines against SARS-CoV-2 are provided.


Assuntos
Antivirais/farmacologia , Produtos Biológicos/farmacologia , Chlamydomonas reinhardtii/genética , Infecções por Coronavirus/tratamento farmacológico , Lectinas/farmacologia , Pneumonia Viral/tratamento farmacológico , Polifenóis/farmacologia , Polissacarídeos/farmacologia , Antivirais/química , Antivirais/isolamento & purificação , Betacoronavirus/efeitos dos fármacos , Betacoronavirus/patogenicidade , Produtos Biológicos/química , Produtos Biológicos/isolamento & purificação , Núcleo Celular/química , Núcleo Celular/genética , Núcleo Celular/metabolismo , Chlamydomonas reinhardtii/química , Chlamydomonas reinhardtii/metabolismo , Cloroplastos/química , Cloroplastos/genética , Cloroplastos/metabolismo , Infecções por Coronavirus/prevenção & controle , Engenharia Genética/métodos , Humanos , Lectinas/química , Lectinas/isolamento & purificação , Coronavírus da Síndrome Respiratória do Oriente Médio/efeitos dos fármacos , Coronavírus da Síndrome Respiratória do Oriente Médio/patogenicidade , Pandemias , Polifenóis/química , Polifenóis/isolamento & purificação , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Vírus da SARS/efeitos dos fármacos , Vírus da SARS/patogenicidade , Síndrome Respiratória Aguda Grave/tratamento farmacológico , Vacinas Virais/biossíntese , Vacinas Virais/farmacologia
3.
Int J Nanomedicine ; 15: 4969-4990, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32764930

RESUMO

Background: Polyphenols possess antioxidant, anti-inflammatory and antimicrobial properties and have been used in the treatment of skin wounds and burns. We previously showed that tannic acid-modified AgNPs sized >26 nm promote wound healing, while tannic acid-modified AgNPs sized 13 nm can elicit strong local inflammatory response. In this study, we tested bimetallic Au@AgNPs sized 30 nm modified with selected flavonoid and non-flavonoid compounds for wound healing applications. Methods: Bimetallic Au@AgNPs were obtained by growing an Ag layer on AuNPs and further modified with selected polyphenols. After toxicity tests and in vitro scratch assay in HaCaT cells, modified lymph node assay as well as the mouse splint wound model were further used to access the wound healing potential of selected non-toxic modifications. Results: Tannic acid, gallic acid, polydatin, resveratrol, catechin, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate and procyanidin B2 used to modify Au@AgNPs exhibited good toxicological profiles in HaCaT cells. Au@AgNPs modified with 15 µM tannic acid, 200 µM resveratrol, 200 µM epicatechin gallate, 1000 µM gallic acid and 200 µM procyanidin B2 induced wound healing in vivo and did not lead to the local irritation or inflammation. Tannic acid-modified Au@AgNPs induced epithelial-to-mesenchymal transition (EMT) - like re-epithelialization, while other polyphenol modifications of Au@AgNPs acted through proliferation and wound closure. Conclusion: Bimetallic Au@AgNPs can be used as a basis for modification with selected polyphenols for topical uses. In addition, we have demonstrated that particular polyphenols used to modify bimetallic nanoparticles may show different effects upon different stages of wound healing.


Assuntos
Ouro/química , Nanopartículas Metálicas/química , Polifenóis/química , Polifenóis/farmacologia , Prata/química , Cicatrização/efeitos dos fármacos , Animais , Antioxidantes/química , Antioxidantes/farmacologia , Biflavonoides/química , Catequina/análogos & derivados , Catequina/química , Camundongos , Proantocianidinas/química , Taninos/química
4.
Food Chem ; 332: 127373, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-32619941

RESUMO

In the present study, effects of riboflavin (RF) and kelp polyphenol extracts (KPE) on mackerel (Scomberomorus Niphonius) myofibrillar protein (MP) gel were studied with or without ultraviolet A (UVA) irradiation treatment. The gel strength was increased with the addition of RF and KPE under UVA irradiation. Analysis of the proteins in the gel indicated that the carbonyl content increased, while the contents of total sulfhydryl and amino groups decreased. The proteins appeared to have no α-helix structures, and the endogenous tryptophan content appeared to decrease. The results of SDS-PAGE indicated that the RF and KPE treated samples under UVA irradiation showed massive MP cross-linking by covalent bonds. Electron spin resonance (ESR) results indicated that UVA irradiation generated free radicals in RF and KPE, which ultimately led to an improvement in MP gel properties. It also indicated that KPE could prevent the occurrence of peroxidation to improve the gel properties.


Assuntos
Proteínas de Peixes/química , Kelp/química , Perciformes , Polifenóis/química , Riboflavina/química , Raios Ultravioleta , Animais , Radicais Livres/química , Géis
5.
Food Chem ; 332: 127467, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-32663755

RESUMO

The mood pyramid of cocoa, which was previously proposed as a new concept, consists of four levels (flavan-3-ols, methylxanthines, minor compounds and orosensory properties). Roasting is a crucial process for flavor development in cocoa but is likely to have a negative impact on the phytochemicals. We investigated the effect of roasting time (10-50 min) and temperature (110-160 °C) on the potential mood-enhancing compounds corresponding to the distinct mood pyramid levels. Phytochemicals were analyzed using UPLC-HRMS, while the flavor was mapped via aroma (HS-SPME-GC-MS) and generic descriptive analysis (trained panel). Results revealed that roasting at 130 °C for 30 min did not significantly affect the levels of epicatechin, procyanidin B2 and theobromine, while salsolinol significantly increased. Moreover, bitterness and astringency were reduced and the desired cocoa flavor was developed. Thus, through selection of appropriate roasting time and temperature conditions phytochemicals of interest could be retained without comprising the flavor.


Assuntos
Cacau/química , Culinária/métodos , Sementes/química , Biflavonoides/química , Catequina/química , Aromatizantes/química , Cromatografia Gasosa-Espectrometria de Massas , Temperatura Alta , Humanos , Odorantes/análise , Polifenóis/química , Proantocianidinas/química , Paladar
6.
PLoS One ; 15(7): e0234509, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32663216

RESUMO

Polyphenols and other potential health-promoting components of sorghum (Sorghum bicolor (L.) Moench) drove its recent growth in the U.S. consumer food industry. Linear sweep (cyclic voltammetry, CV) and differential (cyclic differential pulse) voltammetry methods were developed to detect target polyphenols and amino acids in sweet sorghum juice without interference from the dominant secondary (trans-aconitic acid) and primary (sucrose) metabolites. Of 24 cultivars investigated, No.5 Gambela showed the highest electron-donating capacity, as indicated by the highest peak area, height, and peak anodic potential. Pearson's correlation analysis indicated the contribution of polyphenols (rather than amino acids) on CV voltammograms of juice samples. The Eh-pH values of 173 sweet sorghum juice samples collected in 2017 aligned with quercetin model polyphenol. Accumulation of quercetin-like polyphenols in No.5 Gambela could offer antioxidant-rich juice for conversion to edible syrup as well as an increased tolerance against a recently emerged pest, sugarcane aphid [(Melanaphis sacchari (Zehntner)].


Assuntos
Polifenóis/análise , Sorghum/metabolismo , Grão Comestível , Polifenóis/química , Saccharum/química , Paladar
7.
PLoS One ; 15(6): e0234157, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32516332

RESUMO

Brazilian native fruits are a rich source of polyphenolic compounds that can act as anti-inflammatory and antioxidant agents. Here, we determined the polyphenolic composition, anti-inflammatory mechanism of action, antioxidant activity and systemic toxicity in Galleria mellonella larvae of Eugenia selloi B.D.Jacks. (synonym Eugenia neonitida Sobral) extract (Ese) and its polyphenol-rich fraction (F3) obtained through bioassay-guided fractionation. Phenolic compounds present in Ese and F3 were identified by LC-ESI-QTOF-MS. The anti-inflammatory activity of Ese and F3 was tested in vitro and in vivo through NF-κB activation, cytokine release and neutrophil migration assays. The samples were tested for their effects against reactive species (ROO•, O2•-, HOCl and NO•) and for their toxicity in Galleria mellonella larvae model. The presence of hydroxybenzoic acid, ellagitannins and flavonoids was identified. Ese and F3 reduced NF-κB activation, cytokine release and neutrophil migration, with F3 being three-fold more potent. Overall, F3 exhibited strong antioxidant effects against biologically relevant radicals, and neither Ese nor F3 were toxic to G. mellonella larvae. In conclusion, Ese and F3 revealed the presence of phenolic compounds that decreased the inflammatory parameters evaluated and inhibited reactive oxygen/nitrogen species. E. selloi is a novel source of bioactive compounds that may provide benefits for human health.


Assuntos
Eugenia/química , Frutas/química , Polifenóis/química , Polifenóis/farmacologia , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Quimiocina CXCL2/metabolismo , Depuradores de Radicais Livres/química , Depuradores de Radicais Livres/farmacologia , Depuradores de Radicais Livres/toxicidade , Lepidópteros/efeitos dos fármacos , Masculino , Camundongos , NF-kappa B/metabolismo , Polifenóis/toxicidade , Células RAW 264.7 , Espécies Reativas de Nitrogênio/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Fator de Necrose Tumoral alfa/metabolismo
8.
Food Chem ; 328: 126833, 2020 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-32480265

RESUMO

Raspberries are rich in polyphenols but the impact of organic versus conventional agricultural practices on their phytochemical composition is not well known. This study aimed to analyse and compare the polyphenol accumulation in raspberries grown under both agricultural practices in the same edaphoclimatic conditions. Two raspberry cultivars 'Kweli' and 'Tulameen' were used. Under organic agricultural practices, the polyphenols levels increased for the 'Kweli' cultivar but decreased for the 'Tulameen' cultivar. 'Tulameen' cultivar grown under conventional agricultural practices contained higher anthocyanins levels than grown under organic agricultural practices while for the 'Kweli' cultivar no significant differences were observed between the two agricultural practices. 'Kweli' cultivar presented a significantly higher amount of ellagitannins and ellagic acid derivatives when compared to the 'Tulameen' cultivar under both agricultural practices. The effect of the agricultural practices on the raspberries' chemical profile was dependent on the cultivar and cannot be generalised.


Assuntos
Agricultura , Compostos Fitoquímicos/análise , Rubus/química , Antocianinas/análise , Cromatografia Líquida de Alta Pressão , Ácido Elágico/análise , Frutas/química , Frutas/metabolismo , Taninos Hidrolisáveis/análise , Extratos Vegetais/química , Polifenóis/análise , Polifenóis/química , Rubus/metabolismo
9.
Food Chem ; 329: 127159, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-32497845

RESUMO

The widespread use of neonicotinoids (NEOs) and fipronils (FIPs) as insecticides in modern agriculture has been widely investigated because of their adverse effects on human health. This study aimed to develop an accurate quantitative approach to analyze NEOs and FIPs in tea by using a modified QuEChERS method based on dispersive solid-phase extraction procedure coupled with liquid chromatography-high resolution mass spectrometry. To minimize matrix interferences largely, we selected polyvinylpolypyrrolidone (PVPP) and strong cationic exchange adsorbent (PCX) as dispersive adsorbents to eliminate polyphenols and caffeine from tea extract, respectively. Under optimal conditions, a slight matrix effect was observed for NEOs and FIPs due to the highest reductions of polyphenols and caffeine (94% and 88%, respectively). The method was validated by the European Medicines Agency and Eurachem guidelines and was successfully applied to tea samples for NEOs and FIPs analysis. NEOs and FIPs were commonly detected in tea.


Assuntos
Contaminação de Alimentos/análise , Neonicotinoides/análise , Pirazóis/análise , Chá/química , Cafeína/química , Cromatografia Líquida de Alta Pressão/métodos , Cromatografia Líquida/métodos , Análise de Alimentos/métodos , Inseticidas/análise , Polifenóis/química , Reprodutibilidade dos Testes , Extração em Fase Sólida/métodos , Espectrometria de Massas em Tandem/métodos
10.
Food Chem ; 329: 127158, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-32512394

RESUMO

In order to improve the bioavailability of Juglans regia L. polyphenols (JRP) in human body, the novel Juglans regia L. polyphenols-Hohenbuehelia serotina polysaccharides (HSP) nanoparticles (JRP-HSP NPs) were synthesized based on electrostatic interaction. Moreover, the physicochemical properties, morphologies and gastrointestinal digestive characteristics of JRP-HSP NPs were respectively measured and analyzed. Among four kinds of JRP-HSP NPs prepared by different condition of mass ratio, JRP-HSP NPs (1:16) with encapsulation efficiency of 51.62%, average particle size of 341.0 nm, and zeta potential of -29.3 mv, presented better physicochemical properties, such as thermal stability, photostability and crystalline properties. Morphological analysis revealed JRP-HSP NPs (1:16) appeared spherical and almost uniform. Through evaluation of simulated gastrointestinal digestion, JRP-HSP NPs prevented the variation of phenolic composition induced by gastrointestinal digestion, which was similar as the one of undigested JRP, while un-encapsulated JRP exhibited totally different state due to degradation and biotransformation, suggesting that JRP-HSP NPs possessed the sustained release characteristics in gastrointestinal system. This study provided a non-toxic strategy to encapsulate polyphenols for improving their bioavailability.


Assuntos
Agaricales/química , Polissacarídeos Fúngicos/química , Juglans/química , Nanopartículas/química , Polifenóis/química , Polifenóis/farmacocinética , Disponibilidade Biológica , Digestão , Suco Gástrico/metabolismo , Humanos , Tamanho da Partícula , Polifenóis/síntese química , Saliva , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
11.
Food Chem ; 329: 127219, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-32516714

RESUMO

Plant-based proteins and polyphenols are increasingly being explored as functional food ingredients. Colloidal complexes were prepared from pea protein (PP) and grape seed proanthocyanidin (GSP) and the ability of the PP/GSP complexes to form and stabilize oil-in-water emulsions were investigated. The main interactions between PP and GSP were hydrogen bonding. The stability of PP-GSP complexes to environmental changes were studied: pH (2-9); ion strength (0-0.3 M); and temperature (30-90 °C). Emulsions produced using PP-GSP complexes as emulsifiers had small mean droplet diameters (~200 nm) and strongly negative surface potentials (~-60 mV). Compared to PP alone, PP-GSP complexes slightly decreased the isoelectric point, thermostability, and salt stability of the emulsions, but increased their storage stability. The presence of GSP gave the emulsions a strong salmon (red-yellow) color, which may be beneficial for some specific applications. These results may assist in the creation of more efficacious food-based strategies for delivering proanthocyanidins.


Assuntos
Emulsões/química , Extrato de Sementes de Uva/química , Proteínas de Ervilha/química , Ervilhas/metabolismo , Polifenóis/química , Proantocianidinas/química , Antioxidantes/química , Sítios de Ligação , Calorimetria , Extrato de Sementes de Uva/metabolismo , Ligação de Hidrogênio , Concentração de Íons de Hidrogênio , Simulação de Acoplamento Molecular , Óleos/química , Concentração Osmolar , Proteínas de Ervilha/metabolismo , Polifenóis/metabolismo , Proantocianidinas/metabolismo , Cloreto de Sódio/química , Temperatura , Água/química
12.
Food Chem ; 326: 126760, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32447157

RESUMO

To reveal the characteristic chemical profiles of Pu-erh raw tea (PRT) and traditional green tea (TGT), a high-throughput analytical method based on UPLC-Q-Orbitrap-MS/MS was proposed. 145 components were characterized with a three-level qualitative strategy and the integrated filtering strategy combining nitrogen rule, mass defect, and diagnostic ions information. 124 components were quantified using an internal standard method. The total contents of flavan-3-ols and derivatives, phenolic acids and derivatives were higher in PRT than TGT, while flavonoids were reversed. Furthermore, partial least squares-discriminant analysis (PLS-DA) models were established to classify TGT and PRT. 23 characteristic components were revealed by variable importance in projection method. Their difference in content is between 1.5 and 7.3 times for PRT and TGT. The results showed the chemical characteristics of TGT and PRT clearly and comprehensively. The high-throughput method demonstrated considerable potential in the analysis of complex chemical system, such as food and herbs.


Assuntos
Chá/química , Cromatografia Líquida de Alta Pressão , Análise Discriminante , Hidroxibenzoatos/química , Análise dos Mínimos Quadrados , Polifenóis/química , Espectrometria de Massas em Tandem
13.
Adv Exp Med Biol ; 1195: 77-91, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32468462

RESUMO

Neurodegenerative diseases lead to the death of nerve cells in the brain or the spinal cord. A wide range of diseases are included within the group of neurodegenerative disorders, with the most common ones being dementia, Alzheimer's, and Parkinson's diseases. Millions of older people are suffering from such pathologies. The global increase of life expectancy unavoidably leads to a consequent increase in the number of people who will be at some degree affected by neurodegenerative-related diseases. At this moment, there is no effective therapy or treatment that can reverse the loss of neurons. A growing number of studies highlight the value of the consumption of medical foods, and in particular olive oil, as one of the most important components of the Mediterranean diet. A diet based on extra virgin olive oil seems to contribute toward the lowering of risk of age-related pathologies due to high phenol concentration. The link of a polyphenol found in extra virgin olive oil, namely, tyrosol, with the protein tyrosinase, associated to Parkinson's disease is underlined as a paradigm of affiliation between polyphenols and neurodegenerative disorders.


Assuntos
Doenças Neurodegenerativas/prevenção & controle , Azeite de Oliva/química , Azeite de Oliva/farmacologia , Polifenóis/química , Polifenóis/farmacologia , Dieta Mediterrânea , Humanos , Doenças Neurodegenerativas/dietoterapia , Azeite de Oliva/uso terapêutico , Álcool Feniletílico/análogos & derivados , Álcool Feniletílico/química , Álcool Feniletílico/farmacologia , Álcool Feniletílico/uso terapêutico , Polifenóis/uso terapêutico
14.
Food Chem ; 326: 126968, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32428854

RESUMO

An understanding of the antityrosinase capacity and polyphenols changes during hydrothermal treatments was crucial for application of asparagus. Therefore, asparagus extract was treated at a range of 80-160 °C for 30-150 min in a high temperature reactor. The results suggested that tyrosinase inhibition rate of untreated asparagus extract was recorded as 3.26% but significantly increased to 51.22% and 50.80% after heating for 90 min at 140 °C (lnR0 of 7.21) and 160 °C (lnR0 of 8.57), respectively. The generation and degradation of polyphenols followed the pseudo-first-order kinetic model. The coumaric acid content was increased from 35.03 µg/mL to 307.66 µg/mL at lnR0 of 8.16. The degradation of rutin in asparagus extract was far less compared to that of coumaric acid. Compounds formed were determined by UPLC-Q-TOF-MS yielding main fragments at m/z 451 and 601. In conclusion, hydrothermal treatment was a feasible method for increasing the antityrosinase capacity of asparagus.


Assuntos
Asparagus (Planta)/química , Monofenol Mono-Oxigenase/antagonistas & inibidores , Polifenóis/química , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Indústria de Processamento de Alimentos/métodos , Cinética , Monofenol Mono-Oxigenase/metabolismo , Extratos Vegetais/química , Polifenóis/análise , Polifenóis/farmacologia , Rutina/química , Temperatura
15.
AAPS PharmSciTech ; 21(5): 143, 2020 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-32424702

RESUMO

This work aims to improve the functionality of Rosmarinus officinalis L. (rosemary) polyphenols by encapsulation in an optimized proliposome formulation. A 23 Box-Wilson central composite design (CCD) was employed to determine lone and interaction effects of composition variables on moisture content (Xp); water activity (Aw); concentration and retention of rosemary polyphenols-rosmarinic acid (ROA), carnosol (CAR), and carnosic acid (CNA); and recovery of spray-dried proliposomes (SDP). Processing conditions which generate proliposomes with optimum physicochemical properties were determined by multi-response analysis (desirability approach). Antioxidant and antifungal activities were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH•) sequestering and minimum inhibitory concentration (MIC)/minimum fungicidal concentration (MFC) assays, respectively. SDP exhibited high polyphenol retention, ranging from 62.0 to 100.0% w/w, showing dependence on composition variables and polyphenol lipophilicity. SDP recovery ranged from 20.1 to 45.8%, with Xp and Aw of 1.7 ± 0.14-2.5 ± 0.23% w/w and 0.30 ± 0.004-0.47 ± 0.003, respectively, evidencing product with good chemical and microbiological stability. Optimum liposomal composition was determined, namely, lipid concentration (4.26% w/w), lyophilized extract (LE) concentration (4.48% w/w), and drying aid:(lipid+extract) ratio (7.55% w/w) on wet basis. Relative errors between experimental and predicted values for SDP properties showed concurrence for all responses except CAR retention, being 22% lower. SDP showed high antioxidant activity with IC50 of 9.2 ± 0.2 µg/mL, superior to results obtained for LE (10.8 µg/mL) and butylated hydroxytoluene (BHT), a synthetic antioxidant (12.5 µg/mL). MIC and MFC against Candida albicans (ATCC1023) were 312.5 µg/mL and 1250 µg/mL, respectively, a moderate antimicrobial activity for phytochemical-based products. SDP is shown as a veritable tool to encapsulate hydrophilic and lipophilic rosemary polyphenols generating a product with optimal physicochemical and biological properties.


Assuntos
Lipossomos/química , Polifenóis/química , Rosmarinus/química , Antifúngicos/farmacologia , Antioxidantes/farmacologia , Candida albicans/efeitos dos fármacos , Cápsulas , Dessecação , Composição de Medicamentos , Liofilização , Humanos , Lipídeos/química , Testes de Sensibilidade Microbiana , Folhas de Planta/química , Polifenóis/administração & dosagem , Polifenóis/farmacologia , Água/análise
16.
Anticancer Res ; 40(4): 2025-2032, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32234893

RESUMO

BACKGROUND/AIM: The winemaking procedure results in the generation of stems, a by-product that is harmful to the environment. Concomitantly, stems are rich in polyphenols and, hence, they are putatively beneficial for human health. MATERIALS AND METHODS: In this study, the grape stem extracts derived from three native Greek vine varieties, namely Mavrodaphne, Muscat and Rhoditis were examined for their chemical composition and antioxidant and antimutagenic properties using a battery of in vitro biomarkers. RESULTS: All extracts are rich in polyphenols. Moreover, they exhibit potent antioxidant and antimutagenic properties with the extract of Mavrodaphne being the strongest in reducing the DPPH• and O2 -• radicals and the Fe3+ and in protecting plasmid DNA from peroxyl radical-induced oxidative modification. CONCLUSION: Therefore, although they are serious pollutants, grape stems contain phytochemicals with important biological properties and can be used as (ingredients of) bio-functional foods to improve certain aspects of human health.


Assuntos
Antioxidantes/farmacologia , DNA/efeitos dos fármacos , Polifenóis/farmacologia , Vitis/química , DNA/metabolismo , Dano ao DNA/efeitos dos fármacos , Grécia , Humanos , Oxirredução , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Caules de Planta/química , Plasmídeos/efeitos dos fármacos , Plasmídeos/metabolismo , Polifenóis/química , Espécies Reativas de Oxigênio/metabolismo
17.
Food Chem ; 320: 126630, 2020 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-32224421

RESUMO

The development of a new comprehensive two-dimensional liquid chromatographic method is described, to obtain the profiles of polyphenolic compounds present in olive (Olea europaea L.) leaves and pulps from different genetic origin. Optimisation of the stationary phase nature, particle size, column length and internal diameter, as well as other separation conditions, was performed. Along the study, three stationary phases (C18, PFP and phenyl) in the first dimension (1D), and five (C18, amide, cyano, phenyl and PFP) in the second dimension (2D) were combined to obtain the maximal number of resolved peaks. The optimised method successfully characterised the presence of 26and 29 common polyphenols in olive leaves and pulp extracts, respectively. Peak volume ratios were used to develop linear discriminant analysis models able to distinguish olive leaves and pulp extracts among seven cultivars from several Spanish regions. The results demonstrate that polyphenolic profiles were characteristic of each cultivar.


Assuntos
Olea/química , Extratos Vegetais/química , Polifenóis/química , Cromatografia Líquida de Alta Pressão/métodos , Folhas de Planta/química
18.
Phytomedicine ; 69: 153209, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32240928

RESUMO

BACKGROUND: Since non-alcoholic fatty liver disease (NAFLD) pathogenesis is multi-factorial, pharmacotherapy with a specific target commonly exhibits limited efficacy. Phytotherapy, whose therapeutic efficacy is based on the combined action of several active compounds, offers new treatment opportunity for NAFLD. As a representative, many natural polyphenols could be utilized in phytotherapy for NAFLD. PURPOSE: In present work, we aimed to investigate the therapeutic effects and underlying mechanism of polyphenols in blueberry leaves (PBL) on NAFLD from a mitochondria-centric perspective since mitochondrial dysfunction could play a dominant role in NAFLD. METHODS: Identification and quantification of PBL were performed using liquid chromatography coupled with tandem mass spectrometry. The beneficial effects, especially improving mitochondrial function, and potential mechanism of PBL on NAFLD were studied by in vitro and in vivo study. RESULTS: Polyphenols were abundant in blueberry leaves making it advantaged in NAFLD phytotherapy. PBL effectively alleviated hepatic steatosis, oxidative stress and inflammation as indicated by both in vitro and in vivo study. Furthermore, PBL mediated improvement of mitochondrial dysfunction and antioxidant capability through activation of AMPK/PGC-1α/SIRT3 signaling axis. CONCLUSION: Considering that mitochondrial dysfunction takes precedence over hepatic steatosis and induces NAFLD development, we conclude that PBL improve mitochondrial dysfunction and oxidative defense, subsequently alleviate hepatic steatosis, oxidative stress and inflammation, and eventually alleviate NAFLD.


Assuntos
Mirtilos Azuis (Planta)/química , Mitocôndrias/efeitos dos fármacos , Hepatopatia Gordurosa não Alcoólica/tratamento farmacológico , Polifenóis/análise , Polifenóis/farmacologia , Animais , Antioxidantes/metabolismo , Células Hep G2 , Humanos , Metabolismo dos Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Masculino , Mitocôndrias/metabolismo , Hepatopatia Gordurosa não Alcoólica/metabolismo , Hepatopatia Gordurosa não Alcoólica/fisiopatologia , Estresse Oxidativo/efeitos dos fármacos , Fitoterapia/métodos , Folhas de Planta/química , Polifenóis/química , Ratos Sprague-Dawley
19.
Food Chem ; 324: 126857, 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32344342

RESUMO

Glucagon-like peptide-1 (GLP-1) is an important signal in the peripheral and neural systems, which contributes to the maintenance of glucose and energy homeostasis. In this study, 1H NMR validated polyphenols and polysaccharides extracted from sprouted quinoa yoghurt were used as isolates and conjugates to upregulate the stimulation of GLP-1 release in NCI-H716 cells. In addition, we explored their effect on proglucagon and prohormone convertase 3 mRNA expressions, HNF-3γ and CCK-2R gene protein expression, as well as cytosolic calcium release. Variations in concentration showed a dose-dependent GLP-1 stimulation, and were significantly optimized by germination. Proglucagon mRNA expression in NCI-H716 cells was upregulated, and was relatively highest with QYPSP1 treatments in a 2.68 fold. The results suggested that the conjugates had greater potential to stimulate GLP-1 release than their isolates. Sprouted quinoa yoghurt could therefore be a potential functional food useful to regulate glucose and energy homeostasis.


Assuntos
Chenopodium quinoa/química , Inibidores da Dipeptidil Peptidase IV/química , Peptídeo 1 Semelhante ao Glucagon/metabolismo , Polifenóis/química , Polissacarídeos/química , Iogurte/análise , Cálcio/metabolismo , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Chenopodium quinoa/crescimento & desenvolvimento , Chenopodium quinoa/metabolismo , Cromatografia Líquida de Alta Pressão , Inibidores da Dipeptidil Peptidase IV/metabolismo , Inibidores da Dipeptidil Peptidase IV/farmacologia , Germinação , Humanos , Polifenóis/análise , Polifenóis/isolamento & purificação , Polissacarídeos/análise , Polissacarídeos/isolamento & purificação , Proglucagon/antagonistas & inibidores , Proglucagon/genética , Proglucagon/metabolismo , Pró-Proteína Convertase 1/antagonistas & inibidores , Pró-Proteína Convertase 1/genética , Pró-Proteína Convertase 1/metabolismo
20.
Zhongguo Zhong Yao Za Zhi ; 45(3): 548-554, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32237512

RESUMO

Study the suitability of organic film for salvianolic acid in the ultrafiltration process of Danshen Dizhuye. UPLC was used to analyze the migration of nine phenolic active ingredients in Danshen Dizhuye during ultrafiltration of PES hollow fiber membrane and PS hollow fiber membrane. The structural composition of multi-components was analyzed by three different batches of Danshen Dizhuye before and after ultrafiltration of the two membranes. The results showed that 9 phenolic active ingredients in Danshen Dizhuye did not change significantly after ultrafiltration through PES membrane. However, after ultrafiltration through PS membrane, the content of sodium danshensu, protocatechualdehyde, caffeic acid, 3-hydroxy-4-methoxycinnamic acid and rosmarinic acid in Danshen Dizhuye did not change significantly, while salvianolic acid D, salvianolic acid B and lithospermic acid decreased by about 20%, and the content of salvianolic acid A decreased significantly. The final content in equilibrium was only about 20% of the original solution. Therefore, an in-depth study on the migration particularity of salvianolic acid A in ultrafiltration membrane was the focuse. The results showed that the loss of salvianolic acid A was caused by both membranes during ultrafiltration, and salvianolic acid A was lost more in PS membrane. When the membrane was washed and regenerated, it was found that salvianolic acid A was detected in the ethanol washing solution, but not in the washing liquid, indicating that the loss of salvianolic acid A during the ultrafiltration was mainly adsorptive action. The results suggested that the migration of phenolic active ingredients in Danshen Dizhuye during the membrane ultrafiltration process did not completely follow the molecular weight passing rule of the membrane pore size. At the same time, it may be affected by factors, such as the structure of the membrane material, and the interaction between the membrane structure and the structure of components, and exhibit different migration behaviors during the ultrafiltration of the membrane.


Assuntos
Alcenos/química , Medicamentos de Ervas Chinesas/química , Polifenóis/química , Salvia miltiorrhiza/química , Ultrafiltração , Cromatografia Líquida de Alta Pressão
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