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1.
J Endod ; 46(3): 364-369, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31959482

RESUMO

INTRODUCTION: Diabetes is a common disease that may have some influence on sensory nerves. The aim of this study was to evaluate dental pulp responses to 2 pulp sensibility tests (ie, cold and electric) in patients with type 2 diabetes compared with healthy individuals. METHODS: Three hundred twenty-two premolar teeth in 51 patients who had type 2 diabetes and 347 premolar teeth in 53 individuals with no medical conditions were investigated. The patients with type 2 diabetes were unified and had fasting plasma glucose <300, hemoglobin A1C <10, less than a 10-year history of diabetes mellitus, and no history of hypertension. Electric and cold pulp sensibility tests were performed for all teeth. The cold test results were recorded by the Heft-Parker visual analog scale, and the electric pulp test results were recorded based on the pulp tester's grade that evoked a response. RESULTS: There was no statistically significant difference between upper and lower premolar teeth in healthy individuals compared with the patients with diabetes in response to the cold and electric pulp tests (P > .05). In the patients with diabetes, the response of their upper premolars to the cold test was significantly reduced in diabetic patients >45 years of age (ß = -1.15, P = .013). However, there was no significant correlation between the cold test and age in the lower premolars of both diabetic and nondiabetic participants (P > .05). There was also no significant correlation between the need for a higher number of the electric pulp test current to evoke a response in maxillary and mandibular premolars of the patients with diabetes and nondiabetic participants with age (P > .05). CONCLUSIONS: There was a significant correlation between the reduction of maxillary premolar teeth responses to the cold test in diabetes patients >45 years of age.


Assuntos
Teste da Polpa Dentária , Polpa Dentária , Diabetes Mellitus Tipo 2 , Dente Pré-Molar , Polpa Dentária/fisiopatologia , Diabetes Mellitus Tipo 2/complicações , Humanos , Maxila , Pessoa de Meia-Idade
2.
Int J Mol Sci ; 20(9)2019 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-31071917

RESUMO

The tooth has an unusual sensory system that converts external stimuli predominantly into pain, yet its sensory afferents in teeth demonstrate cytochemical properties of non-nociceptive neurons. This review summarizes the recent knowledge underlying this paradoxical nociception, with a focus on the ion channels involved in tooth pain. The expression of temperature-sensitive ion channels has been extensively investigated because thermal stimulation often evokes tooth pain. However, temperature-sensitive ion channels cannot explain the sudden intense tooth pain evoked by innocuous temperatures or light air puffs, leading to the hydrodynamic theory emphasizing the microfluidic movement within the dentinal tubules for detection by mechanosensitive ion channels. Several mechanosensitive ion channels expressed in dental sensory systems have been suggested as key players in the hydrodynamic theory, and TRPM7, which is abundant in the odontoblasts, and recently discovered PIEZO receptors are promising candidates. Several ligand-gated ion channels and voltage-gated ion channels expressed in dental primary afferent neurons have been discussed in relation to their potential contribution to tooth pain. In addition, in recent years, there has been growing interest in the potential sensory role of odontoblasts; thus, the expression of ion channels in odontoblasts and their potential relation to tooth pain is also reviewed.


Assuntos
Canais Iônicos/genética , Dor/genética , Proteínas Serina-Treonina Quinases/genética , Canais de Cátion TRPM/genética , Dente/fisiopatologia , Polpa Dentária/crescimento & desenvolvimento , Polpa Dentária/fisiopatologia , Dentina/metabolismo , Regulação da Expressão Gênica/genética , Humanos , Neurônios Aferentes/metabolismo , Neurônios Aferentes/patologia , Odontoblastos/metabolismo , Odontoblastos/patologia , Dor/fisiopatologia , Dente/crescimento & desenvolvimento , Gânglio Trigeminal/fisiopatologia
3.
Am J Orthod Dentofacial Orthop ; 155(4): 543-551, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30935609

RESUMO

INTRODUCTION: To evaluate the stress at the apical third of the pulp and neurovascular bundle (NVB) during 5 types of orthodontic movement at different levels of bone loss. Furthermore, correlations among bone loss, orthodontic appliances, and stress increase were assessed. METHODS: Based on cone-beam computed tomography datasets, 10 models of the mandibular second premolar were created. Each of these models was subjected to a gradual horizontal bone loss simulation (0-8 mm). Orthodontic forces of 20 g, 60 g, and 120 g were applied during the finite element analysis (FEA). For each bone loss level, stress values were evaluated with the use of Abaqus at the apical third of the pulp and the NVB. RESULTS: The stress manifested at the apical third of the pulp was smaller than that at the NVB. The highest apical NVB stress was found for rotation (0.000546 N/mm2 for 8 mm bone loss) whereas the lowest stress resulted after translational movements (2.35E-04 MPa for 8 mm bone loss). The FEA showed that Proffit's indicated orthodontic forces did not significantly disturb the pulpal blood flow and damage the apical NVB. Up to a doubling of the NVB stress, bone loss correlated with the force reduction to obtain similar stress levels compared with teeth with no bone loss. CONCLUSIONS: The present findings indicate that the stress manifested at the apical third of the pulp is smaller than that at NVB. Rotational movements induce the highest stress and translational forces develop the lowest stress related to the physiologic capillary blood pressure. Furthermore, in situations with reduced periodontium, lower forces are needed to reach the maximum tolerable stress compared with teeth with intact periodontium.


Assuntos
Polpa Dentária/fisiopatologia , Técnicas de Movimentação Dentária , Adulto , Tomografia Computadorizada de Feixe Cônico , Modelos Dentários , Polpa Dentária/diagnóstico por imagem , Análise do Estresse Dentário , Análise de Elementos Finitos , Humanos , Masculino , Estresse Mecânico , Técnicas de Movimentação Dentária/efeitos adversos
4.
Stomatologiia (Mosk) ; 97(6): 34-37, 2018.
Artigo em Russo | MEDLINE | ID: mdl-30589422

RESUMO

THE PURPOSE OF THE STUDY: determination of the optimal parameters of the action of various types of current, which have the most pronounced irritating effect on the receptor apparatus of the tooth pulp. The study involved 102 volunteers aged 19 to 72 years (53 females and 49 males). The study was conducted on 217 teeth: 86 molars, 25 premolars, 19 canines and 98 incisors. 137 (63%) teeth were intact, in 48 (22%) teeth caries were found, in 32 (15%) teeth there was pulpitis or teeth were depulpated. Electroexcitability of the teeth was determined with the help of various types of electric current: an impulse variable, an impulse constant and a sinusoidal variable. The optimal current for carrying out an electroodontodiagnosis proved to be sinusoidal variable current with a frequency of 50 Hz. This current does not cause polarization of tissues, it is easy to dose, it causes a clear, but not painful sensation, gives the smallest spread of the indicators during repeated studies. The obtained results allowed formulating requirements for electroodontodiagnosis devices.


Assuntos
Cárie Dentária , Polpa Dentária , Estimulação Elétrica , Pulpite , Adulto , Idoso , Dente Pré-Molar , Polpa Dentária/fisiopatologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Dente Molar , Pulpite/diagnóstico , Adulto Jovem
5.
PLoS One ; 13(11): e0207411, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30419075

RESUMO

This study further investigated the mechanisms underlying the rat model of tooth pulp inflammatory pain elicited by complete Freund's adjuvant (CFA), in comparison to other pulpitis models. Pulps of the left maxillary first molars were accessed. In the CFA group, the pulps were exposed, and CFA application was followed by dental sealing. In the open group, the pulps were left exposed to the oral cavity. For the closed group, the pulps were exposed, and the teeth were immediately sealed. Naïve rats were used as negative controls. Several parameters were evaluated at 1, 2, 3 and 8 days. There was no statistical significant difference among the groups when body weight variation, food or water consumption were compared. Analysis of serum cytokines (IL-1ß, TNF or IL-6) or differential blood cell counts did not reveal any evidence of systemic inflammation. The CFA group displayed a significant reduction in the locomotor activity (at 1 and 3 days), associated with an increased activation of satellite glial cells in the ipsilateral trigeminal ganglion (TG; for up to 8 days). Amygdala astrocyte activation was unaffected in any experimental groups. We provide novel evidence indicating that CFA-induced pulp inflammation impaired the locomotor activity, with persistent activation of ipsilateral TG satellite cells surrounding sensory neurons, without any evidence of systemic inflammation or amygdala astrogliosis.


Assuntos
Polpa Dentária , Adjuvante de Freund/efeitos adversos , Células Satélites Perineuronais , Odontalgia , Gânglio Trigeminal , Tonsila do Cerebelo/metabolismo , Tonsila do Cerebelo/patologia , Tonsila do Cerebelo/fisiopatologia , Animais , Citocinas/metabolismo , Polpa Dentária/metabolismo , Polpa Dentária/patologia , Polpa Dentária/fisiopatologia , Adjuvante de Freund/farmacologia , Inflamação/induzido quimicamente , Inflamação/metabolismo , Inflamação/patologia , Inflamação/fisiopatologia , Locomoção , Masculino , Ratos , Ratos Wistar , Células Satélites Perineuronais/metabolismo , Células Satélites Perineuronais/patologia , Odontalgia/induzido quimicamente , Odontalgia/metabolismo , Odontalgia/patologia , Odontalgia/fisiopatologia , Gânglio Trigeminal/metabolismo , Gânglio Trigeminal/patologia , Gânglio Trigeminal/fisiopatologia
6.
PLoS One ; 13(10): e0205534, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30308037

RESUMO

Successful disinfection alongside complete endodontic tissue regeneration and revascularization are the most desired clinical outcomes of regenerative endodontics. Despite reported clinical successes, significant limitations to the current regenerative endodontic procedure (REP) have been elucidated. To improve the current REP, an antibiotics and nitric oxide (NO) releasing biomimetic nanomatrix gel was developed. The study evaluates antibacterial effects of an antibiotics and NO releasing biomimetic nanomatrix gel on multispecies endodontic bacteria. Antibiotics, ciprofloxacin (CF) and metronidazole (MN) were mixed and encapsulated within the NO releasing biomimetic nanomatrix gel. The gel was synthesized and self-assembled from peptide amphiphiles containing various functional groups. Antibacterial effects of the antibiotics and NO releasing biomimetic nanomatrix gel were evaluated using bacterial viability assays involving endodontic microorganisms including clinical samples. Pulp-dentin regeneration was evaluated via animal-model experiments. The antibiotics and NO releasing biomimetic nanomatrix gel demonstrated a concentration dependent antibacterial effect. In addition, NO alone demonstrated a concentration dependent antibacterial effect on endodontic microorganism. An in vivo analysis demonstrated the antibiotics and NO releasing biomimetic nanomatrix gel promoted tooth revascularization with maturation of root canals. An optimal concentration of and NO releasing nanomatrix gel is suggested for its potential as a root treatment material for REP and an appropriate protocol for human trials. Further investigation is required to obtain a larger sample size and decide upon ideal growth factor incorporation.


Assuntos
Antibacterianos/administração & dosagem , Polpa Dentária/efeitos dos fármacos , Dentina/efeitos dos fármacos , Géis , Óxido Nítrico/administração & dosagem , Regeneração/efeitos dos fármacos , Animais , Biofilmes/efeitos dos fármacos , Materiais Biomiméticos/síntese química , Polpa Dentária/diagnóstico por imagem , Polpa Dentária/patologia , Polpa Dentária/fisiopatologia , Dentina/diagnóstico por imagem , Dentina/patologia , Dentina/fisiopatologia , Cães , Sistemas de Liberação de Medicamentos , Enterococcus faecalis/efeitos dos fármacos , Enterococcus faecalis/fisiologia , Géis/síntese química , Infecções por Bactérias Gram-Positivas/diagnóstico por imagem , Infecções por Bactérias Gram-Positivas/tratamento farmacológico , Infecções por Bactérias Gram-Positivas/patologia , Infecções por Bactérias Gram-Positivas/fisiopatologia , Humanos , Nanoestruturas , Neovascularização Fisiológica/efeitos dos fármacos , Neovascularização Fisiológica/fisiologia , Projetos Piloto , Pulpite/diagnóstico por imagem , Pulpite/tratamento farmacológico , Pulpite/patologia , Pulpite/fisiopatologia , Regeneração/fisiologia , Treponema denticola/efeitos dos fármacos , Treponema denticola/fisiologia
7.
Swiss Dent J ; 128(5): 393-399, 2018 May 14.
Artigo em Francês, Alemão | MEDLINE | ID: mdl-29734801

RESUMO

In the context of tooth trauma, mostly the maxillary central front teeth are involved, whereby their roots are in some cases still in the developing stage (immature). Depending on the type of trauma, a necrosis of the pulp is the consequence or at least foreseeable. In order to preserve such a tooth, an endodontic therapy is inevitable. The disadvantage of conventional endodontic therapy methods of immature teeth (apexification, apexogenesis) is the root growth stop. Alternatively, the regeneration/revascularisation of the dental pulp (RP) was suggested, that allows a further root growth. The aim of this case report is to present the procedure of a RP-therapy and to suggest a step-by-step manual.


Assuntos
Necrose da Polpa Dentária/cirurgia , Endodontia/métodos , Incisivo/lesões , Medicina Regenerativa/métodos , Avulsão Dentária/cirurgia , Adolescente , Criança , Polpa Dentária/irrigação sanguínea , Polpa Dentária/fisiopatologia , Necrose da Polpa Dentária/fisiopatologia , Seguimentos , Humanos , Incisivo/fisiopatologia , Incisivo/cirurgia , Lábio/lesões , Lábio/cirurgia , Masculino , Avulsão Dentária/fisiopatologia , Reimplante Dentário/métodos
8.
Mol Pain ; 14: 1744806918763270, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29448913

RESUMO

Background The mechanisms underlying tooth pulp hypersensitivity associated with masseter muscle hyperalgesia remain largely underinvestigated. In the present study, we aimed to determine whether masseter muscle contraction induced by daily electrical stimulation influences the mechanical head-withdrawal threshold and genioglossus electromyography activity caused by the application of capsaicin to the upper first molar tooth pulp. We further investigated whether astroglial glutamine synthesis is involved in first molar tooth pulp hypersensitivity associated with masseter muscle contraction. Methods The first molar tooth pulp was treated with capsaicin or vehicle in masseter muscle contraction or sham rats, following which the astroglial glutamine synthetase inhibitor methionine sulfoximine or Phosphate buffered saline (PBS) was applied. Astroglial activation was assessed via immunohistochemistry. Results The mechanical head-withdrawal threshold of the ipsilateral masseter muscle was significantly decreased in masseter muscle contraction rats than in sham rats. Genioglossus electromyography activity was significantly higher in masseter muscle contraction rats than sham rats. Glial fibrillary acidic protein-immunoreactive cell density was significantly higher in masseter muscle contraction rats than in sham rats. Administration of methionine sulfoximine induced no significant changes in the density of glial fibrillary acidic protein-immunoreactive cells relative to PBS treatment. However, mechanical head-withdrawal threshold was significantly higher in masseter muscle contraction rats than PBS-treated rats after methionine sulfoximine administration. Genioglossus electromyography activity following first molar tooth pulp capsaicin treatment was significantly lower in methionine sulfoximine-treated rats than in PBS-treated rats. In the ipsilateral region, the total number of phosphorylated extracellular signal-regulated protein kinase immunoreactive cells in the medullary dorsal horn was significantly smaller upon first molar tooth pulp capsaicin application in methionine sulfoximine-treated rats than in PBS-treated rats. Conclusions Our results suggest that masseter muscle contraction induces astroglial activation, and that this activation spreads from caudal to the obex in the medullary dorsal horn, resulting in enhanced neuronal excitability associated with astroglial glutamine synthesis in medullary dorsal horn neurons receiving inputs from the tooth pulp. These findings provide significant insight into the mechanisms underlying tooth pulp hypersensitivity associated with masseter muscle contraction.


Assuntos
Astrócitos/metabolismo , Polpa Dentária/metabolismo , Polpa Dentária/patologia , Glutamina/metabolismo , Músculo Masseter/fisiopatologia , Bulbo/metabolismo , Contração Muscular , Animais , Astrócitos/efeitos dos fármacos , Capsaicina/farmacologia , Polpa Dentária/efeitos dos fármacos , Polpa Dentária/fisiopatologia , Estimulação Elétrica , Eletromiografia , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Proteína Glial Fibrilar Ácida/metabolismo , Hiperalgesia/patologia , Hiperalgesia/fisiopatologia , Masculino , Músculo Masseter/efeitos dos fármacos , Músculo Masseter/patologia , Bulbo/efeitos dos fármacos , Bulbo/fisiopatologia , Metionina Sulfoximina/administração & dosagem , Metionina Sulfoximina/farmacologia , Dente Molar/patologia , Contração Muscular/efeitos dos fármacos , Fosforilação/efeitos dos fármacos , Células do Corno Posterior/efeitos dos fármacos , Células do Corno Posterior/metabolismo , Ratos Sprague-Dawley
9.
J Endod ; 44(3): 395-404, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29336883

RESUMO

INTRODUCTION: In this retrospective study, we investigated long-term (over 3 years) follow-up results of teeth that exhibited contradictory results between the pulp sensibility test (thermal or electric pulp test) and ultrasound Doppler flowmetry (UDF) until 1 year after trauma to inspect the prognosis of the pulp. METHODS: Data were collected from the records of trauma patients in our hospital between February 2012 and May 2015. The teeth that had continuously shown contrasting results on the pulp sensibility test and UDF until 1 year after trauma were chosen for the study. Cases with follow-up records of more than 3 years after trauma were finally included, and a retrospective chart review was performed. RESULTS: Data from 343 teeth in 147 patients who visited the hospital with traumatic dental injuries were examined. Among these, 13 teeth from 7 patients were included, and the record of each case was reviewed. All the subjects showed negative responses on the pulp sensibility test and positive responses on UDF until 1 year after trauma. Ultimately, 8 of the 13 teeth recovered pulp sensibility. Two teeth failed to recover pulp sensibility and became symptomatic; root canal treatment was performed on the teeth. The remaining 3 teeth belonged to patients suffering from nerve damage; therefore, the pulp sensibility test was not feasible. CONCLUSIONS: Based on this study, UDF can be effectively used for the evaluation of pulpal status in traumatized teeth.


Assuntos
Polpa Dentária/diagnóstico por imagem , Polpa Dentária/fisiopatologia , Fluxometria por Laser-Doppler , Traumatismos Dentários/diagnóstico por imagem , Ultrassonografia Doppler , Adolescente , Adulto , Feminino , Seguimentos , Humanos , Masculino , Prognóstico , Estudos Retrospectivos , Fatores de Tempo , Adulto Jovem
10.
Int Endod J ; 51 Suppl 2: e94-e106, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28294357

RESUMO

AIM: To investigate in human dental pulp fibroblasts (HDPF) the expression of factors involved in dental pulp physiopathological processes and in an experimental model of cell activation called nemosis, and to compare the behaviour of pulp cell activation with sound lung fibroblast MRC5, employed as a reference model for nemosis. METHODOLOGY: Nemotic response was induced in three-dimensional cultures of HDPF and lung fibroblasts. The expressions of molecules involved in physiological (alkaline phosphatase, type I collagen) and in inflammatory processes (IL-6, CXCL8, CCL20, COX-2) were studied using real-time PCR. Concentrations of IL-6 and CXCL8 were analysed during 4 days with ELISA. Nonparametric tests were used to determine statistical differences between groups. RESULTS: A significant decrease (P < 0.001) in type I collagen and alkaline phosphatase was observed in MRC5 and HDPF nemotic responses. Although the amounts of mRNA differed between these cell types, there was an increase in CCL20, CXCL8 and COX-2 expression (P < 0.001). Unlike HDPF, MRC5 spheroids displayed significant amounts of IL-6 concentrations and mRNA expression. Notably, increased concentrations of CXCL8 were recorded in all three-dimensional cultures compared with monolayers as a function of time (P < 0.05). CONCLUSION: Although the nemotic responses observed were not identical in the pulpal and lung fibroblasts, similarities occurred in the expression of chemokines and cyclooxygenase-2. Nemotic reactions and inflammatory processes in pulp diseases share similarities in terms of the expression of factors. Thus, this in vitro model could constitute a powerful tool to study intercellular relations within the dental pulp and to develop new local treatments to counteract the inflammatory reaction that occurs during pulpitis.


Assuntos
Morte Celular/fisiologia , Polpa Dentária/fisiopatologia , Fibroblastos/fisiologia , Fosfatase Alcalina/metabolismo , Quimiocina CCL20/metabolismo , Colágeno Tipo I/metabolismo , Ciclo-Oxigenase 2/metabolismo , Polpa Dentária/citologia , Polpa Dentária/metabolismo , Ensaio de Imunoadsorção Enzimática , Fibroblastos/metabolismo , Citometria de Fluxo , Expressão Gênica/fisiologia , Humanos , Interleucina-6/metabolismo , Interleucina-8/metabolismo , Reação em Cadeia da Polimerase em Tempo Real
11.
Oral Dis ; 24(4): 619-627, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29117466

RESUMO

OBJECTIVE: To investigate physical characteristics and behaviours of dental pulp cells of teeth isolated from a dentinogenesis imperfecta (DGI) patient with a novel dentin sialophosphoprotein (DSPP) mutation. SUBJECTS AND METHODS: Whole exome and Sanger sequencing were employed to identify mutations. Physical characteristics of the teeth were examined. Pulp cells' behaviours including cell proliferation, colony-forming unit, osteogenic differentiation, pluripotent markers, and mesenchymal stem cell markers were investigated. RESULTS: The proband had opalescent brown primary teeth with extensive loss of enamel. Mutation analysis revealed a novel heterozygous 4-bp deletion, c.1915_1918delAAGT (p.K639QfsX674), in exon 5 of the DSPP associated with DGI. Analysis of the extracted primary incisor demonstrated a decrease in brightness but an increase in yellow and red chroma. The dentin showed reduced mineral density. The dentinal tubules were present in the predentin, but progressively collapsed in the dentin. The pulp cells exhibited markedly reduced CD105 expression, decreased cell proliferation, and smaller colony-forming units. CONCLUSIONS: We identified a novel mutation in the DSPP gene which disturbed dentin characteristics and pulp cells' behaviours. Our study expands the mutation spectrum and understanding of pathologic dentin phenotypes related to the frameshift deletion in the dentin phosphoprotein (DPP) region of the DSPP gene.


Assuntos
Sequência de Bases , Polpa Dentária/patologia , Polpa Dentária/fisiopatologia , Dentinogênese Imperfeita/genética , Proteínas da Matriz Extracelular/genética , Fosfoproteínas/genética , Deleção de Sequência , Sialoglicoproteínas/genética , Diferenciação Celular/genética , Proliferação de Células/genética , Células Cultivadas , Pré-Escolar , Ensaio de Unidades Formadoras de Colônias , Polpa Dentária/ultraestrutura , Dentina/fisiopatologia , Dentina/ultraestrutura , Endoglina/metabolismo , Humanos , Masculino , Linhagem , Fenótipo
12.
Ultrasound Med Biol ; 43(9): 1963-1974, 2017 09.
Artigo em Inglês | MEDLINE | ID: mdl-28651921

RESUMO

The aim of the study was to investigate the effects of low-intensity pulsed ultrasound (LIPUS) on dentoalveolar structures during application of force to a cultured mandible slice taken from an ovariectomized rat model of osteoporosis. Rats were divided based on whether they had ovariectomy and/or LIPUS application into four groups: control osteoporosis group, control normal group, ultrasound-treated osteoporosis group and ultrasound-treated normal group. The mandibles were dissected, sliced and cultured before application of a 0.5-N force. Tissue specimens from five rats per group received LIPUS; the remaining rats served as untreated controls. Tissue sections were evaluated histologically and histomorphometrically. Osteoporosis significantly affected the alveolar bone without any effect on the dentin-pulp complex. LIPUS enhanced osteoporotic alveolar bone remodeling and increased cementum and predentin thickness. Furthermore, LIPUS application significantly increased odontoblast and periodontal ligament cell counts (p < 0.05) in both groups. Therefore, LIPUS enhances alveolar bone remolding and increases cementum and predentin formation in osteoporotic rat mandible slice organ cultures.


Assuntos
Polpa Dentária/fisiopatologia , Mandíbula/fisiopatologia , Osteoporose/fisiopatologia , Ondas Ultrassônicas , Animais , Modelos Animais de Doenças , Feminino , Ratos , Ratos Wistar
13.
Int Endod J ; 50(8): 729-739, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27520405

RESUMO

AIM: To determine the prevalence of preoperative endodontic pain (PREP) and the incidence of postoperative endodontic pain (POEP), identifying the predictors of PREP and POEP in a southern Brazilian subpopulation, using clinical data from an electronic chart database (ECD). METHODOLOGY: This retrospective observational study included 563 consecutive individuals presenting for root canal treatment (RCT). Patients were treated by undergraduate and graduate students, following standard RCT protocols. Demographic, medical and dental variables were extracted from a pre-structured and standardized ECD. The main outcomes PREP and incident POEP were collected through a 0-10 numeric rating scale, dichotomized as none/mild (<4) or moderate/severe (≥4) pain. Predictive models calculating the prevalence ratios (PR) of PREP and the relative risks (RR) of incident POEP were carried out with Poisson regression analysis, estimating the relationship between clinical factors, PREP and incident POEP. RESULTS: Mean age at baseline was 49.2 ± 17.1 years, with 68.4% women. The prevalence and incidence of moderate/severe PREP and POEP were 44.4% and 3.8%, respectively. RCT intervention significantly reduced PREP (P < 0.001). Multivariate analysis revealed that group of teeth, location (mandibular teeth), pulpitis, necrotic pulp, preoperative swelling and periapical radiolucency were independently associated with moderate/severe PREP, whilst age ≥60 years and root canal retreatments were independent protective factors to PREP (P < 0.05). No demographic, medical or dental variables were associated with POEP, although molar teeth (RR = 4.23, 95%CI = 0.93-19.2, P = 0.056) had a borderline nonsignificant association. CONCLUSIONS: Moderate/severe PREP was independently associated with age, group of teeth, location, preoperative swelling, retreatments and pulp and periapical status. No demographic, medical or dental variable predicted moderate/severe POEP following RCT amongst this subpopulation.


Assuntos
Doenças da Polpa Dentária/fisiopatologia , Doenças da Polpa Dentária/cirurgia , Polpa Dentária/fisiopatologia , Dor Facial/etiologia , Dor Pós-Operatória/etiologia , Tratamento do Canal Radicular/efeitos adversos , Brasil/epidemiologia , Registros Eletrônicos de Saúde , Dor Facial/epidemiologia , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Dor Pós-Operatória/epidemiologia , Prevalência , Estudos Retrospectivos
14.
J Oral Rehabil ; 44(4): 291-298, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28032898

RESUMO

Throughout lifetime, the teeth are continuously exposed to numerous chemical and physical impacts, which cause the wear of the dental hard tissues, gingival recession and other oral changes with sometimes subsequent problems. Age-related wear of tooth surfaces reduces the dental enamel thickness and exposes deeper layers of enamel, which have different physical and chemical properties than the surface enamel. Gingival recession is the main causal factor of root caries and dentine hypersensitivity. Age-related changes in dentine include the formation of secondary dentine and the reduction in tubular lumen diameter (dentine sclerosis), which lead to a reduction in the volume of the pulp chamber. In addition to the reduction in the volume of pulp chamber, changes to the dental pulp also include dental pulp calcifications. The age-related physiological changes to the teeth should be carefully distinguished from pathological changes, especially when they induce pain or a negative impact on the oral health-related quality of life (OHRQoL) of the older individuals. Therefore, regular oral examinations coupled with early preventive measures should aim at maintaining oral health until old age.


Assuntos
Envelhecimento/fisiologia , Dente/anatomia & histologia , Dente/fisiologia , Esmalte Dentário/patologia , Polpa Dentária/anatomia & histologia , Polpa Dentária/patologia , Polpa Dentária/fisiologia , Polpa Dentária/fisiopatologia , Calcificações da Polpa Dentária/patologia , Exposição da Polpa Dentária/patologia , Exposição da Polpa Dentária/fisiopatologia , Sensibilidade da Dentina/patologia , Sensibilidade da Dentina/fisiopatologia , Retração Gengival/patologia , Humanos , Dente/patologia , Dente/fisiopatologia
15.
Beijing Da Xue Xue Bao Yi Xue Ban ; 48(5): 878-883, 2016 10 18.
Artigo em Chinês | MEDLINE | ID: mdl-27752174

RESUMO

OBJECTIVE: To compare the proliferation and osteoblastic differentiation of dental pulp stem cell (DPSC) isolated from normal and inflamed pulps of different degrees in Beagle immature premolars, and provide evidence for the use of inflammatory DPSC (IDPSC). METHODS: This study evaluated 14 Beagle's young premolars (21 roots). In the experiment group, irreversible pulpitis was induced by pulp exposure and the inflamed pulps were extracted 2 weeks and 6 weeks after the pulp chamber opening.For the control group, normal pulps were extracted immediately after the exposure. HE staining and real-time PCR were performed to confirm the inflammation. The cells were isolated from the inflamed and normal pulps (IDPSC and DPSC). Cell proliferation and osteoblastic differentiation potentials of the two cells were compared. RESULTS: Inflammation cells infiltration was observed in the inflamed pulps by HE staining. The expression of inflammatory factor was much higher in the 6 week inflamed pulp. IDPSC had higher potential of cell proliferation and osteoblastic differentiation potentials. Furthermore, the osteoblastic differentiation potentials of IDPSC from 2 week inflamed pulp were higher than those from 6 week inflamed pulp. CONCLUSION: The potential of cell proliferation and osteoblastic differentiation of DPSC was enhanced at early stage of irreversible pulpitis, and reduced at late stage in Beagle immature premolars.


Assuntos
Polpa Dentária/citologia , Polpa Dentária/fisiopatologia , Inflamação/fisiopatologia , Pulpite/fisiopatologia , Células-Tronco Adultas/citologia , Células-Tronco Adultas/fisiologia , Animais , Dente Pré-Molar/citologia , Dente Pré-Molar/fisiopatologia , Diferenciação Celular/fisiologia , Proliferação de Células/fisiologia , Cães , Osteoblastos/citologia , Osteoblastos/fisiologia , Reação em Cadeia da Polimerase em Tempo Real
16.
J Endod ; 42(11): 1626-1632, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27639638

RESUMO

INTRODUCTION: The purpose of this study was to ascertain the regenerative characteristics of apical papilla-derived cells (APDCs) from immature teeth with pulpal and periapical pathosis and thus to provide proof-of-principle evidence for further regenerative endodontic research. METHODS: Pulpal and periapical pathosis was induced in immature permanent double-rooted premolars of beagles, which were randomly assigned to experimental treatment groups: group AO (n = 14), pulp disruption and access left open; group PS (n = 14), supragingival plaque suspension-soaked cotton pellet was introduced, and access was sealed; and control (n = 7), untreated. The teeth were extracted at 2- and 4-week periods after experimental treatments. APDCs were cultured from the extracted teeth, and their cellular proliferation, differentiation characteristics, and stemness were assessed. The data were statistically analyzed. RESULTS: After 4 weeks of intentional pulpal and periapical pathosis induction period, all teeth in group PS showed features of apical periodontitis with necrotic pulp, and their APDCs showed significantly increased proliferation rate and osteogenic/odontogenic differentiation capabilities (P < .05). The stemness was maintained in all APDCs, although the stem cell population was smaller in group PS at 2-week period when the inflammatory responses were most fulminant (P < .05). CONCLUSIONS: The APDCs from immature teeth retained the regenerative characteristics with the differences according to their pulpal and periapical pathosis. The results of this study partly provide the evidence for regenerative endodontic research.


Assuntos
Papila Dentária/fisiopatologia , Tecido Periapical/fisiopatologia , Regeneração/fisiologia , Animais , Dente Pré-Molar/patologia , Células Cultivadas , Citocinas/biossíntese , Papila Dentária/patologia , Polpa Dentária/patologia , Polpa Dentária/fisiopatologia , Necrose da Polpa Dentária/patologia , Necrose da Polpa Dentária/fisiopatologia , Necrose da Polpa Dentária/terapia , Cães , Modelos Animais , Odontogênese , Osteogênese , Periodontite Periapical/patologia , Periodontite Periapical/fisiopatologia , Periodontite Periapical/terapia , Tecido Periapical/patologia
17.
Braz Oral Res ; 30(1)2016 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-27556552

RESUMO

Revascularization of immature teeth with necrotic pulps traditionally involves the use of triple antibiotic paste, which may sometimes lead to undesirable complications. The objective of this study was to assess tissue repair in immature dog teeth with apical periodontitis subjected to revascularization, comparing two different pastes used for root canal disinfection. Apical periodontitis was induced in 30 dog premolars. Teeth were randomly divided into three experimental groups: root canals filled with triple antibiotic paste (n = 10); root canals filled with 1% propolis paste (n = 10); and no medication (n = 10). An additional group (n = 10, no intervention) was used as control. After 7 months, the jaws were histologically evaluated for the following variables: newly formed mineralized tissue (present/absent); vital tissue in the canal space (absent/periodontal ligament-like/pulp-like); apical extension of root (present/absent); and severity of inflammatory process (absent/mild/moderate/severe). There were no statistically significant differences among the experimental groups in new mineralized tissue formation and apical root development. The formation of vital tissue in the canal space, in turn, was statistically different between the triple paste and propolis groups: vital tissues were present in all revascularized teeth disinfected with propolis paste (100%), compared to 71% of those disinfected with the triple paste. Severity of inflammatory process was different between the triple paste and no medication groups. The new tissues formed onto canal walls and in the root canal space showed characteristics of cementum and periodontal ligament, respectively. Propolis may have some advantages over the triple paste for the revascularization of immature teeth.


Assuntos
Anti-Infecciosos/farmacologia , Necrose da Polpa Dentária/tratamento farmacológico , Regeneração Tecidual Guiada/métodos , Neovascularização Fisiológica/efeitos dos fármacos , Periodontite Periapical/tratamento farmacológico , Própole/farmacologia , Irrigantes do Canal Radicular/farmacologia , Dente/irrigação sanguínea , Animais , Anti-Infecciosos/uso terapêutico , Polpa Dentária/efeitos dos fármacos , Polpa Dentária/fisiopatologia , Cavidade Pulpar/efeitos dos fármacos , Cavidade Pulpar/fisiopatologia , Necrose da Polpa Dentária/fisiopatologia , Dentina/efeitos dos fármacos , Cães , Pomadas , Periodontite Periapical/fisiopatologia , Ligamento Periodontal/efeitos dos fármacos , Própole/uso terapêutico , Distribuição Aleatória , Reprodutibilidade dos Testes , Irrigantes do Canal Radicular/uso terapêutico , Fatores de Tempo , Ápice Dentário/efeitos dos fármacos , Ápice Dentário/fisiopatologia , Remineralização Dentária/métodos , Resultado do Tratamento
18.
J Dent Res ; 95(11): 1274-81, 2016 10.
Artigo em Inglês | MEDLINE | ID: mdl-27384335

RESUMO

Human dental pulp stem cells (DPSCs) can be isolated from inflamed pulp derived from carious teeth with symptomatic irreversible pulpitis (I-DPSCs), which possess stemness and multidifferentiation potentials similar to DPSCs from healthy pulp. Since macrophages-essential cell players of the pulpal innate immunity-can regulate pulpal inflammation and repair, the authors investigated the immunomodulatory effects of DPSCs/I-DPSCs on macrophage functions and their underlying mechanisms. Similar to DPSCs, I-DPSCs were capable of colony-forming efficiency and adipogenic and osteo/dentinogenic differentiation under in vitro induction conditions. I-DPSCs also expressed a similar phenotypic profile of mesenchymal stem cell markers, except a relatively higher level of CD146 as compared with DPSCs. Coculture of DPSCs or I-DPSCs with differentiated THP-1 cells, the human monocyte cell line, markedly suppressed tumor necrosis factor α (TNF-α) secretion in response to stimulation with lipopolysaccharides (LPS) and/or nigericin. However, unlike TNF-α, the secreted level of interleukin 1ß was not affected by coculture with DPSCs or I-DPSCs. Furthermore, DPSC/I-DPSC-mediated inhibition of TNF-α secretion by macrophages was abolished by pretreatment with 1-methyl-D-tryptophan, a specific inhibitor of indoleamine-pyrrole 2,3-dioxygenase (IDO), but not by NSC-398, a specific inhibitor of COX-2, suggesting IDO as a mediator. Interestingly, IDO expression was significantly augmented in macrophages and mesenchymal stromal cells in inflamed human pulp tissues. Collectively, these findings show that I-DPSCs, similar to DPSCs, possess stem cell properties and suppress macrophage functions via the TNF-α/IDO axis, thereby providing a physiologically relevant context for their innate immunomodulatory activity in the dental pulp and their capability for pulp repair.


Assuntos
Polpa Dentária/citologia , Indolamina-Pirrol 2,3,-Dioxigenase/fisiologia , Macrófagos/fisiologia , Pulpite/fisiopatologia , Células-Tronco/fisiologia , Fator de Necrose Tumoral alfa/fisiologia , Adolescente , Adulto , Western Blotting , Células Cultivadas , Inibidores de Ciclo-Oxigenase 2/farmacologia , Polpa Dentária/fisiopatologia , Ensaio de Imunoadsorção Enzimática , Humanos , Indolamina-Pirrol 2,3,-Dioxigenase/antagonistas & inibidores , Indolamina-Pirrol 2,3,-Dioxigenase/metabolismo , Interleucina-1beta/metabolismo , Pessoa de Meia-Idade , Adulto Jovem
19.
Mol Med Rep ; 14(3): 1891-900, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27432616

RESUMO

The anterograde intraflagellar transport motor protein, kif3a, regulates the integrity of primary cilia and various cellular functions, however, the role of kif3a in dental mesenchymal stem/precursor cell differentiation remains to be fully elucidated. In the present study, the expression of kif3a was knocked down in human dental follicle cells (hDFCs) and human dental pulp cells (hDPCs) using short hairpin RNA. The results of subsequent immunofluorescence revealed that knocking down kif3a resulted in the loss of primary cilia, which led to impairment of substantial mineralization and expression of the differentiation­associated markers, including alkaline phosphatase, Runt­related transcription factor 2, dentin matrix protein 1 and dentin sialophosphoprotein in the hDFCs and hDPCs. The results of reverse transcription­quantitative polymerase chain reaction and western blot analyses showed that the expression levels of Wnt3a­mediated active ß­catenin and lymphoid enhancer­binding factor 1 were attenuated, whereas the expression of phosphorylated glycogen synthase kinase 3ß was enhanced, in the kif3a­knockdown cells. In addition, exogenous Wnt3a partially rescued osteoblastic differentiation in the hDFCs and hDPCs. These results demonstrated that inhibition of kif3a in the hDFCs and hDPCs disrupted primary cilia formation and/or function, and indicated that kif3a is important in the differentiation of hDFCs and hDPCs through the Wnt pathway. These findings not only enhance current understanding of tooth development and diseases of tooth mineralization, but also indicate possible strategies to regulate mineralization during tooth repair and regeneration.


Assuntos
Diferenciação Celular , Polpa Dentária/citologia , Cinesina/fisiologia , Células-Tronco Mesenquimais/metabolismo , Via de Sinalização Wnt , Adolescente , Células Cultivadas , Polpa Dentária/metabolismo , Polpa Dentária/fisiopatologia , Humanos , Células-Tronco Mesenquimais/fisiologia , Odontogênese , Osteoblastos
20.
J Endod ; 42(6): 935-42, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27118600

RESUMO

INTRODUCTION: Endodontic diagnostic tests are often used clinically to assess pulp status as a basis for the diagnosis and determination of whether root canal treatment (RCT) is indicated. Response to cold and pain on percussion are 2 common tests, yet their validity in identifying nonvital pulp in regular dental practice has not been reported. METHODS: We assessed the validity of cold and percussion tests to identify nonvital pulp in teeth requiring RCT in a dental practice setting performed by 46 general dentists and 16 endodontists in the National Dental Practice-Based Research Network. The influence of patient-, tooth-, and dentist-related characteristics was investigated. Observed bleeding from the pulp chamber was the clinical reference. Sensitivity (SN), specificity (SP), overall test accuracy (TA), positive (PPV) and negative (NPV) predictive values, and likelihood and diagnostic odds ratios (LR+, LR-, dORs) were calculated for each single test and the combined cold and percussion tests. RESULTS: Seven hundred eight patient teeth were included. Cold test showed high validity to identify a nonvital pulp status (SN = 89%, SP = 80%, TA = 84%, PPV = 81%, NPV = 88%, LR+ = 4.35, LR- = 0.14, dOR = 31.4), whereas pain on percussion had lower validity (SN = 72%, SP = 41%, TA = 56%, PPV = 54%, NPV = 60%, LR+ = 1.22, LR- = 0.69, dOR = 1.78). Combining the 2 tests did not increase validity, whereas preoperative pain, medication intake, patient age and sex, and dentist training level affected test validity significantly. CONCLUSIONS: In regular dental practice, the cold test exhibits higher validity to discriminate between vital and nonvital pulp than the tooth percussion test.


Assuntos
Necrose da Polpa Dentária/diagnóstico , Teste da Polpa Dentária/métodos , Polpa Dentária/fisiopatologia , Percussão/métodos , Adulto , Fatores Etários , Idoso , Temperatura Baixa , Dinamarca , Cavidade Pulpar/patologia , Teste da Polpa Dentária/normas , Pesquisa em Odontologia , Sensibilidade da Dentina/diagnóstico , Odontólogos/educação , Testes Diagnósticos de Rotina/métodos , Testes Diagnósticos de Rotina/estatística & dados numéricos , Endodontistas/educação , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Razão de Chances , Dor , Percussão/estatística & dados numéricos , Valor Preditivo dos Testes , Reprodutibilidade dos Testes , Tratamento do Canal Radicular/métodos , Sensibilidade e Especificidade , Fatores Sexuais , Suécia , Capacitação de Professores , Estados Unidos
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