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1.
Molecules ; 26(16)2021 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-34443339

RESUMO

The treatment of environmental pollution by microorganisms and their enzymes is an innovative and socially acceptable alternative to traditional remediation approaches. Microbial biodegradation is often characterized with high efficiency as this process is catalyzed via degrading enzymes. Various naturally isolated microorganisms were demonstrated to have considerable ability to mitigate many environmental pollutants without external intervention. However, only a small fraction of these strains are studied in detail to reveal the mechanisms at the enzyme level, which strictly limited the enhancement of the degradation efficiency. Accordingly, this review will comprehensively summarize the function of various degrading enzymes with an emphasis on catalytic mechanisms. We also inspect the expanded applications of these pollutant-degrading enzymes in industrial processes. An in-depth understanding of the catalytic mechanism of enzymes will be beneficial for exploring and exploiting more degrading enzyme resources and thus ameliorate concerns associated with the ineffective biodegradation of recalcitrant and xenobiotic contaminants with the help of gene-editing technology and synthetic biology.


Assuntos
Biocatálise , Poluentes Ambientais/metabolismo , Enzimas/metabolismo , Poluentes Ambientais/isolamento & purificação
2.
Chem Biol Interact ; 345: 109574, 2021 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-34228969

RESUMO

NAD(P)H: Quinone Oxidoreductase 1 (NQO1) is an antioxidant enzyme that catalyzes the two-electron reduction of several different classes of quinone-like compounds (quinones, quinone imines, nitroaromatics, and azo dyes). One-electron reduction of quinone or quinone-like metabolites is considered to generate semiquinones to initiate redox cycling that is responsible for the generation of reactive oxygen species and oxidative stress and may contribute to the initiation of adverse drug reactions and adverse health effects. On the other hand, the two-electron reduction of quinoid compounds appears important for drug activation (bioreductive activation) via chemical rearrangement or autoxidation. Two-electron reduction decreases quinone levels and opportunities for the generation of reactive species that can deplete intracellular thiol pools. Also, studies have shown that induction or depletion (knockout) of NQO1 were associated with decreased or increased susceptibilities to oxidative stress, respectively. Moreover, another member of the quinone reductase family, NRH: Quinone Oxidoreductase 2 (NQO2), has a significant functional and structural similarity with NQO1. The activity of both antioxidant enzymes, NQO1 and NQO2, becomes critically important when other detoxification pathways are exhausted. Therefore, this article summarizes the interactions of NQO1 and NQO2 with different pharmacological agents, endogenous biochemicals, and environmental contaminants that would be useful in the development of therapeutic approaches to reduce the adverse drug reactions as well as protection against quinone-induced oxidative damage. Also, future directions and areas of further study for NQO1 and NQO2 are discussed.


Assuntos
Antioxidantes/metabolismo , Poluentes Ambientais/metabolismo , NAD(P)H Desidrogenase (Quinona)/metabolismo , Preparações Farmacêuticas/metabolismo , Quinona Redutases/metabolismo , Transporte de Elétrons , Humanos , Ligação Proteica
3.
Environ Sci Technol ; 55(14): 9926-9937, 2021 07 20.
Artigo em Inglês | MEDLINE | ID: mdl-34180659

RESUMO

We aimed to assess the relationships among the adipose tissue's (AT) oxidative microenvironment, in situ accumulated persistent organic pollutant (POP) concentrations, and cancer development. POP and oxidative stress levels were quantified in AT samples from 382 adults recruited within the GraMo cohort (2003-2004) in Granada (Spain). The 16-year cancer incidence was ascertained by reviewing health/administrative databases. Cox-regression models and mediation analyses were performed. The enzymes superoxide dismutase (SOD) and glutathione reductase (GRd) were positively associated with the risk of non-hormone-dependent (NHD) cancer [adjusted hazard ratio (HR) 1.76; 95% confidence interval (CI): 1.17, 2.64 and HR 2.35; 95% CI: 1.41, 3.94, respectively]. After adjustment for covariates, polychlorinated biphenyl-138 (PCB-138) (HR 1.78; 95% CI: 1.03, 3.09), ß-hexachlorocyclohexane (ß-HCH) (HR 1.70; 95% CI: 1.09, 2.64), and hexachlorobenzene (HR 1.54; 95% CI: 1.02, 2.33) were also positively associated with the risk of NHD cancer. Although confidence intervals included the null value, probably because of the modest number of cancer cases, we observed a potential mediation effect of SOD and GRd on the associations between ß-HCH and the risk of NHD tumors (percent mediated = 33 and 47%, respectively). Our results highlight the relevance of human AT's oxidative microenvironment as a predictor of future cancer risk as well as its potential mediating role on POP-related carcinogenesis. Given their novelty, these findings should be interpreted with caution and confirmed in future studies.


Assuntos
Poluentes Ambientais , Hidrocarbonetos Clorados , Neoplasias , Praguicidas , Bifenilos Policlorados , Tecido Adiposo/metabolismo , Adulto , Poluentes Ambientais/metabolismo , Humanos , Hidrocarbonetos Clorados/metabolismo , Incidência , Neoplasias/induzido quimicamente , Neoplasias/epidemiologia , Oxirredução , Poluentes Orgânicos Persistentes , Praguicidas/metabolismo , Bifenilos Policlorados/metabolismo , Microambiente Tumoral
4.
Int J Mol Sci ; 22(11)2021 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-34070927

RESUMO

Citric acid (CA), as an organic chelator, plays a vital role in alleviating copper (Cu) stress-mediated oxidative damage, wherein a number of molecular mechanisms alter in plants. However, it remains largely unknown how CA regulates differentially abundant proteins (DAPs) in response to Cu stress in Brassica napus L. In the present study, we aimed to investigate the proteome changes in the leaves of B. L. seedlings in response to CA-mediated alleviation of Cu stress. Exposure of 21-day-old seedlings to Cu (25 and 50 µM) and CA (1.0 mM) for 7 days exhibited a dramatic inhibition of overall growth and considerable increase in the enzymatic activities (POD, SOD, CAT). Using a label-free proteome approach, a total of 6345 proteins were identified in differentially treated leaves, from which 426 proteins were differentially expressed among the treatment groups. Gene ontology (GO) and KEGG pathways analysis revealed that most of the differential abundance proteins were found to be involved in energy and carbohydrate metabolism, photosynthesis, protein metabolism, stress and defense, metal detoxification, and cell wall reorganization. Our results suggest that the downregulation of chlorophyll biosynthetic proteins involved in photosynthesis were consistent with reduced chlorophyll content. The increased abundance of proteins involved in stress and defense indicates that these DAPs might provide significant insights into the adaptation of Brassica seedlings to Cu stress. The abundances of key proteins were further verified by monitoring the mRNA expression level of the respective transcripts. Taken together, these findings provide a potential molecular mechanism towards Cu stress tolerance and open a new route in accelerating the phytoextraction of Cu through exogenous application of CA in B. napus.


Assuntos
Brassica napus/efeitos dos fármacos , Ácido Cítrico/farmacologia , Cobre/toxicidade , Poluentes Ambientais/toxicidade , Proteínas de Plantas/genética , Proteoma/genética , Adaptação Fisiológica , Brassica napus/genética , Brassica napus/crescimento & desenvolvimento , Brassica napus/metabolismo , Catalase/genética , Catalase/metabolismo , Clorofila/biossíntese , Ácido Cítrico/metabolismo , Cobre/metabolismo , Poluentes Ambientais/antagonistas & inibidores , Poluentes Ambientais/metabolismo , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Ontologia Genética , Redes e Vias Metabólicas/efeitos dos fármacos , Redes e Vias Metabólicas/genética , Anotação de Sequência Molecular , Peroxidases/classificação , Peroxidases/genética , Peroxidases/metabolismo , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/genética , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Proteínas de Plantas/classificação , Proteínas de Plantas/metabolismo , Proteoma/classificação , Proteoma/metabolismo , Plântula/efeitos dos fármacos , Plântula/genética , Plântula/crescimento & desenvolvimento , Plântula/metabolismo , Estresse Fisiológico , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismo
5.
Ecotoxicol Environ Saf ; 219: 112363, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34087735

RESUMO

Persistent organic pollutant (POPs) contamination was analyzed in samples collected from wild and captive giant pandas to characterize seasonal variation in concentrations of POPs and possible sources. POP concentrations in bamboo and fecal samples collected from captive pandas showed significant fluctuations compared with those collected from wild pandas in each season. The highest polychlorinated biphenyl (PCB) and organochlorine pesticide (OCP) concentrations were 1380 pg g-1 dw and 3140 pg g-1 dw, respectively, which were observed in captive bamboo samples in the summer. PCBs varied seasonally, whereas OCPs did not show apparent seasonal variation. Based on the seasonal variability, component analysis, and the positive matrix factorization results, we determined that the secondary volatilization of POPs during periods of high temperatures was the leading cause of the exposure of pandas to pollutants (45%), and atmospheric transport played a crucial role in the secondary distribution of pollutants in panda food. The other two sources of pollution were historical residues transmitted over long distances to protected areas (28%), as well as UP-POPs and new inputs from agricultural activities (27%). The concentrations of pollutants in bamboo shoots were significantly lower than those in bamboo. Therefore, bamboo shoots should be incorporated into the diet of captive pandas in the spring to reduce their exposure to pollutants. The absorption capacity of pollutants associated with the consumption of bamboo shoots was significantly lower than that associated with the consumption of bamboo. The diet of young captive pandas in the summer should also be managed with caution given their slightly stronger ability to absorb pollutants.


Assuntos
Exposição Ambiental/estatística & dados numéricos , Poluentes Ambientais/metabolismo , Poluição Ambiental/estatística & dados numéricos , Ursidae/metabolismo , Animais , Dieta , Monitoramento Ambiental , Bifenilos Policlorados , Estações do Ano
6.
Ecotoxicol Environ Saf ; 220: 112376, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34051661

RESUMO

Previous studies suggest that cadmium (Cd) is one of the causative factors of prostate cancer (PCa), but the effect of chronic Cd exposure on PCa progression remains unclear. Besides, whether long noncoding RNAs (lncRNAs) are involved in the regulation of prolonged exposure to Cd in PCa needs to be elucidated. In the present study, we found that the serum concentration of Cd in PCa patients was positively correlated with the Gleason score and tumor-node-metastasis (TNM) classification. To simulate chronic Cd exposure in PCa, we subjected PC3 and DU145 cells to long-term, low-dose Cd exposure and further examined tumor behavior. Functional studies identified that chronic Cd exposure promoted cell growth and ferroptosis resistance in vitro and in vivo. Furthermore, we found that lncRNA OIP5-AS1 expression was greatly elevated in PC3 and DU145 cells upon chronic Cd exposure. Dysregulation of OIP5-AS1 expression mediated cell growth and Cd-induced ferroptosis. Mechanistically, we demonstrated that OIP5-AS1 served as an endogenous sponge of miR-128-3p to regulate the expression of SLC7A11, a surrogate marker of ferroptosis. Moreover, miR-128-3p decreased cell viability by enhancing ferroptosis. Taken together, our data indicate that lncRNA OIP5-AS1 promotes PCa progression and ferroptosis resistance through miR-128-3p/SLC7A11 signaling.


Assuntos
Sistema y+ de Transporte de Aminoácidos/metabolismo , Cádmio/toxicidade , Poluentes Ambientais/toxicidade , Ferroptose/efeitos dos fármacos , MicroRNAs/metabolismo , Neoplasias da Próstata/metabolismo , RNA Longo não Codificante/metabolismo , Idoso , Idoso de 80 Anos ou mais , Cádmio/metabolismo , Linhagem Celular Tumoral , Proliferação de Células , Poluentes Ambientais/metabolismo , Regulação Neoplásica da Expressão Gênica , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias da Próstata/genética , RNA Longo não Codificante/genética , Transdução de Sinais
7.
Ecotoxicol Environ Saf ; 217: 112256, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-33901779

RESUMO

Non-alcoholic fatty liver disease (NAFLD) has been the most common chronic liver disease in the world, including the developing countries. NAFLD is metabolic disease with significant lipid deposition in the hepatocytes of the liver, which is usually associated with oxidative stress, inflammation and fibrogenesis, and insulin resistance. Progressive NAFLD can develop into non-alcoholic steatohepatitis (NASH) or hepatocellular carcinoma. The current evidence proposes that environmental pollutants promote development and progression of NAFLD, and autophagy plays a vital role but is multifactorial affected in NAFLD. In this review, we analyzed on the regulations of common environmental pollutants on autophagy in NAFLD. To clarify the involved roles of autophagy, we discussed the dysregulation of autophagy by environmental pollutants in adipose tissue and gut, and their interactions with liver, as well as epigenetic regulation on autophagy by environmental pollutants. Furthermore, protective roles of potential therapeutic treatments on the multiple-hits of autophagy in NAFLD were descripted.


Assuntos
Autofagia/fisiologia , Poluentes Ambientais/toxicidade , Hepatopatia Gordurosa não Alcoólica/fisiopatologia , Tecido Adiposo/metabolismo , Carcinoma Hepatocelular/metabolismo , Poluentes Ambientais/metabolismo , Epigênese Genética , Hepatócitos/metabolismo , Humanos , Inflamação/metabolismo , Resistência à Insulina/fisiologia , Fígado/metabolismo , Neoplasias Hepáticas/patologia , Hepatopatia Gordurosa não Alcoólica/induzido quimicamente , Estresse Oxidativo
8.
Microb Cell Fact ; 20(1): 72, 2021 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-33736647

RESUMO

The large-scale application of organic pollutants (OPs) has contaminated the air, soil, and water. Persistent OPs enter the food supply chain and create several hazardous effects on living systems. Thus, there is a need to manage the environmental levels of these toxicants. Microbial glycoconjugates pave the way for the enhanced degradation of these toxic pollutants from the environment. Microbial glycoconjugates increase the bioavailability of these OPs by reducing surface tension and creating a solvent interface. To date, very little emphasis has been given to the scope of glycoconjugates in the biodegradation of OPs. Glycoconjugates create a bridge between microbes and OPs, which helps to accelerate degradation through microbial metabolism. This review provides an in-depth overview of glycoconjugates, their role in biofilm formation, and their applications in the bioremediation of OP-contaminated environments.


Assuntos
Bactérias/metabolismo , Biodegradação Ambiental , Poluentes Ambientais/metabolismo , Glicoconjugados/metabolismo , Bactérias/crescimento & desenvolvimento , Biofilmes/crescimento & desenvolvimento , Tensoativos/metabolismo
9.
Appl Environ Microbiol ; 87(11)2021 05 11.
Artigo em Inglês | MEDLINE | ID: mdl-33741618

RESUMO

Sphingomonas wittichii RW1 is one of a few strains known to grow on the related compounds dibenzofuran (DBF) and dibenzo-p-dioxin (DXN) as the sole source of carbon. Previous work by others (B. Happe, L. D. Eltis, H. Poth, R. Hedderich, and K. N. Timmis, J Bacteriol 175:7313-7320, 1993, https://doi.org/10.1128/jb.175.22.7313-7320.1993) showed that purified DbfB had significant ring cleavage activity against the DBF metabolite trihydroxybiphenyl but little activity against the DXN metabolite trihydroxybiphenylether. We took a physiological approach to positively identify ring cleavage enzymes involved in the DBF and DXN pathways. Knockout of dbfB on the RW1 megaplasmid pSWIT02 results in a strain that grows slowly on DBF but normally on DXN, confirming that DbfB is not involved in DXN degradation. Knockout of SWIT3046 on the RW1 chromosome results in a strain that grows normally on DBF but that does not grow on DXN, demonstrating that SWIT3046 is required for DXN degradation. A double-knockout strain does not grow on either DBF or DXN, demonstrating that these are the only ring cleavage enzymes involved in RW1 DBF and DXN degradation. The replacement of dbfB by SWIT3046 results in a strain that grows normally (equal to the wild type) on both DBF and DXN, showing that promoter strength is important for SWIT3046 to take the place of DbfB in DBF degradation. Thus, both dbfB- and SWIT3046-encoded enzymes are involved in DBF degradation, but only the SWIT3046-encoded enzyme is involved in DXN degradation.IMPORTANCE S. wittichii RW1 has been the subject of numerous investigations, because it is one of only a few strains known to grow on DXN as the sole carbon and energy source. However, while the genome has been sequenced and several DBF pathway enzymes have been purified, there has been very little research using physiological techniques to precisely identify the genes and enzymes involved in the RW1 DBF and DXN catabolic pathways. Using knockout and gene replacement mutagenesis, our work identifies separate upper pathway ring cleavage enzymes involved in the related catabolic pathways for DBF and DXN degradation. The identification of a new enzyme involved in DXN biodegradation explains why the pathway of DBF degradation on the RW1 megaplasmid pSWIT02 is inefficient for DXN degradation. In addition, our work demonstrates that both plasmid- and chromosomally encoded enzymes are necessary for DXN degradation, suggesting that the DXN pathway has only recently evolved.


Assuntos
Proteínas de Bactérias/química , Benzofuranos/metabolismo , Dioxinas/metabolismo , Dioxigenases/química , Poluentes Ambientais/metabolismo , Sphingomonas/metabolismo , Proteínas de Bactérias/metabolismo , Biodegradação Ambiental , Dioxigenases/metabolismo , Sphingomonas/enzimologia
10.
Ecotoxicol Environ Saf ; 215: 112174, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33773155

RESUMO

BACKGROUND: Phthalates, which are used as excipients of drugs, have been related to adverse reproductive outcomes. However, the relationships between medication use and phthalate exposure among women undergoing in vitro fertilization (IVF) have not been studied. OBJECTIVE: To investigate the associations between the medication intake and phthalate metabolites in urine and follicular fluid (FF). METHOD: Eight phthalate metabolites were measured in urine and FF samples from 274 women undergoing IVF using liquid chromatography-tandem mass spectrometry. Information on recent medication intake was obtained via interview by trained staff. We constructed generalized linear regression models to examine the associations of medication intake with phthalate metabolite concentrations and dose-response relationships between the number of medicines used and metabolite concentrations in two matrices. RESULTS: Four of 10 drugs were used by more than 10% of the participants, including vitamins (23.0%), traditional Chinese medicine (TCM, 22.3%), antioxidants (12.4%) and amoxicillin (10.2%). Participants who had used TCM had 26.0% (95% CI: 0.0, 58.8%), 32.6% (95% CI: 4.2, 68.8%) and 32.3% (95% CI: 2.6, 70.6%) higher urinary mono-n-butyl phthalate (MBP), mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) and mono(2-ethyl-5-oxohexyl) phthalate (MEOHP) concentrations, respectively, than those who had not. Antioxidant intake was associated with a 30.6% (95% CI: -48.5, -6.6%) decrease in the urinary MBP concentration. Compared with non-users, women who reported the use of medicines had 53.2% (95% CI: 2.7, 128.5%) higher concentrations of MMP and a 37.7% (95% CI: -60.7, -1.5%) lower level of MBP in FF, respectively. CONCLUSION: Our data suggest that the intake of some medications may increase phthalate exposure among women undergoing IVF.


Assuntos
Poluentes Ambientais/metabolismo , Líquido Folicular/metabolismo , Ácidos Ftálicos/metabolismo , Adulto , Antioxidantes/análise , Cromatografia Líquida , Exposição Ambiental/análise , Exposição Ambiental/estatística & dados numéricos , Poluentes Ambientais/análise , Poluentes Ambientais/urina , Feminino , Fertilização In Vitro , Líquido Folicular/química , Humanos , Espectrometria de Massas , Pessoa de Meia-Idade , Ácidos Ftálicos/urina , Reprodução , Vitamina A , Vitaminas , Adulto Jovem
11.
Arch Microbiol ; 203(5): 2463-2473, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33677632

RESUMO

Petroleum hydrocarbon contaminants, which are among the most serious pollutants in the petroleum industry, can be degraded sufficiently by Pseudomonas aeruginosa. However, temperature-induced stress will severely inhibit this biodegradation. In this study, the proteome of P. aeruginosa P6 at 25 °C, 43 °C and 37 °C was used to examine the impact of temperature on the molecular mechanism of biodegradation of petroleum hydrocarbon by P. aeruginosa P6. Differentially expressed proteins were identified by iTRAQ technology, and the functions of these proteins were identified by bioinformatic analysis. The impact of 25 °C and 43 °C on cellular processes has also been discussed. The results showed that the expression of proteins in chemotaxis toward petroleum hydrocarbons, terminal oxidation of aromatic rings in petroleum hydrocarbons and trans-membrane transport of fatty acids and nutriments were clearly inhibited under 25 °C condition. The expression of proteins in chemotaxis, emulsification, adhesion and terminal oxidation of petroleum hydrocarbons; catalysis of fatty alcohols and fatty aldehydes; trans-membrane transport of nutriments and ß-oxidation were clearly inhibited under 43 °C condition.


Assuntos
Poluentes Ambientais/metabolismo , Hidrocarbonetos/metabolismo , Petróleo/metabolismo , Proteoma/metabolismo , Pseudomonas aeruginosa/metabolismo , Proteínas de Bactérias/metabolismo , Biodegradação Ambiental , Oxirredução , Estresse Fisiológico , Temperatura
12.
Ecotoxicol Environ Saf ; 212: 112009, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33556811

RESUMO

Cu pollution is a problem in mining areas in Peru. Here we evaluate the phytoextraction capacity, physiological and proteomic responses of four species growing in copper-contaminated areas in Arequipa, Peru. The plants used in the experiments were obtained by collecting seedlings (Tessaria integrifolia, Bacharis salicifolia), rhizomes (Eleocharis montevidensis) and seeds (Chenopodium murale) along a polluted river. They were exposed to solutions containing 2, 4, 8, 16 and 32 mg Cu L-1 during 20 days. Growth was affected in a concentration-dependent way. According to the tolerance index, B. salicifolia and C. murale were the most sensitive species, but with greater Cu phytoextraction capacity and accumulation in the biomass. The content and ratio of photosynthetic pigments changed differently for each specie and carotenoids level were less affected than chlorophyll. Cu also induced changes in the protein and sugar contents. Antioxidant enzyme activities (catalase and superoxide dismutase) increased with a decrease in the malondialdehyde. There were marked changes in the protein 2D-PAGE profiles with an increase in the abundance of metallothioneins (MT) of class II type I and II. Our results suggest that these species can grow in Cu polluted areas because they developed multiple tolerance mechanisms, such as and MTs production seems a important one.


Assuntos
Adaptação Biológica/efeitos dos fármacos , Cobre/toxicidade , Poluentes Ambientais/toxicidade , Metalotioneína/metabolismo , Desenvolvimento Vegetal/efeitos dos fármacos , Poluentes do Solo/toxicidade , Antioxidantes/metabolismo , Biodegradação Ambiental , Biomassa , Clorofila/metabolismo , Cobre/metabolismo , Poluentes Ambientais/metabolismo , Mineração , Peru , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/metabolismo , Proteômica , Plântula/efeitos dos fármacos , Plântula/crescimento & desenvolvimento , Plântula/metabolismo , Poluentes do Solo/metabolismo , Especificidade da Espécie
13.
Ecotoxicol Environ Saf ; 213: 112050, 2021 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-33621748

RESUMO

Bisphenol A (BPA), an endocrine disruptor, exist in almost all waters. In the present study, we expose adult male Gobiocypris rarus rare minnow to 15 µg/L BPA to study the effect BPA on fish hepatic lipid metabolism. Following 1, 3 and 5 weeks exposure, the liver tissue of rare minnow was separated. The change of the hepatic morphology, hepatosomatic index, lipid composition and expression of lipid metabolism related genes were analyzed through paraffin section, oil red O staining, lipidomic analysis, and quantitative real-time PCR. BPA can cause significant hepatic lipid deposition in male rare minnow, leading to an increase in triglyceride (TG) level (1.84-22.87-fold), but it is also accompanied by a decrease in diglyceride level (1.67-4.78-fold). The expression of lipid metabolism related genes showed that BPA exposure can up-regulate TG synthesis related genes expression, and down-regulate TG degradation genes expression. Expression of TG transport related genes were also disrupted by BPA. It suggests that BPA can up-regulate rare minnow hepatic TG level through multi-path, and ultimately lead to lipid accumulation in the liver. The results of the present study enrich the mechanisms of environmental endocrine disruptors affecting lipid accumulation in fish.


Assuntos
Compostos Benzidrílicos/toxicidade , Cyprinidae/metabolismo , Poluentes Ambientais/metabolismo , Poluentes Ambientais/toxicidade , Fígado/metabolismo , Fenóis/toxicidade , Triglicerídeos/metabolismo , Animais , Disruptores Endócrinos/metabolismo , Feminino , Expressão Gênica , Metabolismo dos Lipídeos , Fígado/efeitos dos fármacos , Masculino
14.
Ecotoxicol Environ Saf ; 213: 112040, 2021 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-33610943

RESUMO

Among different synthetic compounds copper (Cu) is persistently and frequently used as growth promoter, antibacterial, antifungal and antiparasitic agent and has become common environmental pollutant. Therefore, this study explores the cardio-toxic effects of control group (10 mg/kg bw Cu) and treatment group (125 and 250 mg/kg bw Cu), and it association with process of autophagy and metabolomics in myocardium of pigs kept in three different experimental treatments for a period of 80 days. The results of serum biochemical parameters showed a significantly increase in creatinine kinase (CK), creatine kinase-MB (CK-MB), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C) and aspartate aminotransferase (AST) in pigs exposed to 125 mg/kg bw and 250 mg/kg bw Cu. Meanwhile, the severe structural abnormalities in cardiomyocytes were found when exposed to 250 mg/kg Cu at day 80. In addition, the mRNA and proteins (Beclin1, ATG5 and LC3II) expression levels were significantly increased and p62 was significantly decreased in cardiomyocytes exposed to 250 mg/kg Cu at day 80 of the trial. Further, UPLC-QTOF/MS technique showed that 7 metabolites were up-regulated and 37 metabolites were down-regulated in cardiomyocytes after 250 mg/kg Cu treatment, with a principal impact on the metabolic pathways including glycerophospholipid metabolism, one carbon pool by folate, fatty acid elongation and fatty acid degradation, which were related to autophagy. Overall, our study identified the autophagy processes and metabolites in metabolic pathways in Cu-induced myocardium injury, which provided useful evidence of myocardium toxicity caused by Cu exposure via metabolomics and multiple bioanalytic methods.


Assuntos
Autofagia/efeitos dos fármacos , Cobre/toxicidade , Poluentes Ambientais/toxicidade , Coração/efeitos dos fármacos , Animais , Poluentes Ambientais/metabolismo , Coração/fisiologia , Redes e Vias Metabólicas , Metabolômica , Miocárdio/metabolismo , Suínos
15.
Artigo em Inglês | MEDLINE | ID: mdl-33593243

RESUMO

The initial cyanide (CN-) concentration and amount of co-contaminants in GCTs can inhibit bacterial growth and reduce the CN--degrading ability of bacteria. Several microorganisms can biotransform a wide range of organic and inorganic industrial contaminants into nontoxic compounds. However, active enzymatic CN- metabolism processes are mostly constrained by the physical and chemical characteristics of GCTs. High concentrations of toxic metal co-contaminants, such as, Pb, and Cr, and factors, such as pH, temperature, and oxygen concentration create oxidative stress and limit the CN--degrading potential of cyanotrophic strains. The effects of such external and internal factors on the CN--degrading ability of bacteria hinder the selection of suitable microorganisms for CN- biodegradation. Therefore, understanding the effects of the physicochemical properties of GCTs on cyanobacteria strains can help identify suitable microbes and favorable environmental conditions to promote microbial growth and can also help design efficient CN- biodegradation processes. In this review, we present a detailed analysis of the physicochemical properties of GCTs and their effects on microbial CN- degradation.


Assuntos
Cianetos/toxicidade , Cianobactérias/efeitos dos fármacos , Poluentes Ambientais/toxicidade , Ouro/toxicidade , Biodegradação Ambiental/efeitos dos fármacos , Cianetos/química , Cianetos/metabolismo , Cianobactérias/crescimento & desenvolvimento , Cianobactérias/metabolismo , Poluentes Ambientais/química , Poluentes Ambientais/metabolismo , Ouro/química , Ouro/metabolismo , Resíduos Industriais/análise
16.
Molecules ; 26(4)2021 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-33562176

RESUMO

In the last 3 years alone, over 10,000 publications have appeared on the topic of dye removal, including over 300 reviews. Thus, the topic is very relevant, although there are few articles on the practical applications on an industrial scale of the results obtained in research laboratories. Therefore, in this review, we focus on advanced oxidation methods integrated with biological methods, widely recognized as highly efficient treatments for recalcitrant wastewater, that have the best chance of industrial application. It is extremely important to know all the phenomena and mechanisms that occur during the process of removing dyestuffs and the products of their degradation from wastewater to prevent their penetration into drinking water sources. Therefore, particular attention is paid to understanding the mechanisms of both chemical and biological degradation of dyes, and the kinetics of these processes, which are important from a design point of view, as well as the performance and implementation of these operations on a larger scale.


Assuntos
Corantes/isolamento & purificação , Corantes/metabolismo , Poluentes Ambientais/isolamento & purificação , Poluentes Ambientais/metabolismo , Biodegradação Ambiental , Oxirredução
17.
Int J Biol Macromol ; 177: 58-82, 2021 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-33577817

RESUMO

Lignin peroxidase (LiP) seems to be a catalyst for cleaving high-redox potential non-phenolic compounds with an oxidative cleavage of CC and COC bonds. LiP has been picked to seek a practical and cost-effective alternative to the sustainable mitigation of diverse environmental contaminants. LiP has been an outstanding tool for catalytic cleaning and efficient mitigation of environmental pollutants, including lignin, lignin derivatives, dyes, endocrine-disrupting compounds (EDCs), and persistent organic pollutants (POPs) for the past couple of decades. The extended deployment of LiP has proved to be a promising method for catalyzing these environmentally related hazardous pollutants of supreme interest. The advantageous potential and capabilities to act at different pH and thermostability offer its working tendencies in extended environmental engineering applications. Such advantages led to the emerging demand for LiP and increasing requirements in industrial and biotechnological sectors. The multitude of the ability attributed to LiP is triggered by its stability in xenobiotic and non-phenolic compound degradation. However, over the decades, the catalytic activity of LiP has been continuing in focus enormously towards catalytic functionalities over the available physiochemical, conventional, catalyst mediated technology for catalyzing such molecules. To cover this literature gap, this became much more evident to consider the catalytic attributes of LiP. In this review, the existing capabilities of LiP and other competencies have been described with recent updates. Furthermore, numerous recently emerged applications, such as textile effluent treatment, dye decolorization, catalytic elimination of pharmaceutical and EDCs compounds, have been discussed with suitable examples.


Assuntos
Lignina/metabolismo , Peroxidases/metabolismo , Biodegradação Ambiental/efeitos dos fármacos , Catálise , Corantes/metabolismo , Poluentes Ambientais/metabolismo , Humanos , Xenobióticos/metabolismo
18.
Toxicol Appl Pharmacol ; 416: 115444, 2021 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-33549591

RESUMO

Health disparities exist dependent on socioeconomic status, living conditions, race/ethnicity, diet, and exposures to environmental pollutants. Herein, the various exposures contributing to a person's exposome are collectively considered social determinants of health (SDOH), and the SDOH-exposome impacts health more than health care. This review discusses the extent of evidence of the physiologic consequences of these exposures at the intracellular level. We consider how the SDOH-exposome, which captures how individuals live, work and age, induces cell processes that modulate a conceptual "redox rheostat." Like an electrical resistor, the SDOH-exposome, along with genetic predisposition and age, regulate reductive and oxidative (redox) stress circuits and thereby stimulate inflammation. Regardless of the source of the SDOH-exposome that induces chronic inflammation and immunosenescence, the outcome influences cardiometabolic diseases, cancers, infections, sepsis, neurodegeneration and autoimmune diseases. The endogenous redox rheostat is connected with regulatory molecules such as NAD+/NADH and SIRT1 that drive redox pathways. In addition to these intracellular and mitochondrial processes, we discuss how the SDOH-exposome can influence the balance between metabolism and regulation of immune responsiveness involving the two main molecular drivers of inflammation, the NLRP3 inflammasome and NF-κB induction. Mitochondrial and inflammasome activities play key roles in mediating defenses against pathogens and controlling inflammation before diverse cell death pathways are induced. Specifically, pyroptosis, cell death by inflammation, is intimately associated with common disease outcomes that are influenced by the SDOH-exposome. Redox influences on immunometabolism including protein cysteines and ion fluxes are discussed regarding health outcomes. In summary, this review presents a translational research perspective, with evidence from in vitro and in vivo models as well as clinical and epidemiological studies, to outline the intracellular consequences of the SDOH-exposome that drive health disparities in patients and populations. The relevance of this conceptual and theoretical model considering the SARS-CoV-2 pandemic are highlighted. Finally, the case of asthma is presented as a chronic condition that is modified by adverse SDOH exposures and is manifested through the dysregulation of immune cell redox regulatory processes we highlight in this review.


Assuntos
Disparidades nos Níveis de Saúde , Mediadores da Inflamação/metabolismo , Líquido Intracelular/metabolismo , Estresse Oxidativo/fisiologia , Determinantes Sociais da Saúde/tendências , Poluentes Ambientais/efeitos adversos , Poluentes Ambientais/imunologia , Poluentes Ambientais/metabolismo , Humanos , Mediadores da Inflamação/imunologia , Líquido Intracelular/imunologia , Pesquisa Médica Translacional/métodos , Pesquisa Médica Translacional/tendências
19.
Molecules ; 26(2)2021 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-33477459

RESUMO

In this study, a laccase LAC-Yang1 was successfully purified from a white-rot fungus strain Pleurotus ostreatus strain yang1 with high laccase activity. The enzymatic properties of LAC-Yang1 and its ability to degrade and detoxify chlorophenols such as 2,6-dichlorophenol and 2,3,6-trichlorophenol were systematically studied. LAC-Yang1 showed a strong tolerance to extremely acidic conditions and strong stability under strong alkaline conditions (pH 9-12). LAC-Yang1 also exhibited a strong tolerance to different inhibitors (EDTA, SDS), metal ions (Mn2+, Cu2+, Mg2+, Na+, K+, Zn2+, Al3+, Co2+, and metal ion mixtures), and organic solvents (glycerol, propylene glycol). LAC-Yang1 showed good stability in the presence of Mg2+, Mn2+, glycerol, and ethylene glycol. Our results reveal the strong degradation ability of this laccase for high concentrations of chlorophenols (especially 2,6-dichlorophenol) and chlorophenol mixtures (2,6-dichlorophenol + 2,3,6-trichlorophenol). LAC-Yang1 displayed a strong tolerance toward a variety of metal ions (Na2+, Zn2+, Mn2+, Mg2+, K+ and metal ion mixtures) and organic solvents (glycerol, ethylene glycol) in its degradation of 2,6-dichlorophenol and 2,3,6-trichlorophenol. The phytotoxicity of 2,6-dichlorophenol treated by LAC-Yang1 was significantly reduced or eliminated. LAC-Yang1 demonstrated a good detoxification effect on 2,6-dichlorophenol while degrading this compound. In conclusion, LAC-Yang1 purified from Pleurotus ostreatus has great application value and potential in environmental biotechnology, especially the efficient degradation and detoxification of chlorophenols.


Assuntos
Biodegradação Ambiental , Clorofenóis/química , Clorofenóis/metabolismo , Poluentes Ambientais/metabolismo , Lacase/metabolismo , Pleurotus/enzimologia , Pleurotus/crescimento & desenvolvimento
20.
Environ Res ; 195: 110764, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33497679

RESUMO

Humans are exposed to a myriad of chemical substances in both occupational and environmental settings. Persistent organic pollutants (POPs) have drawn attention for their adverse effects including cancer and endocrine disruption. Herein, the objectives were 1) to describe serum and adipose tissue retinol levels, along with serum retinol binding protein 4 (RBP4) concentrations, and 2) to assess the associations of adipose tissue POP levels with these retinoid parameters, as well as their potential interaction with the previously-observed POP-related disruption of redox microenvironment. Retinol was measured in both serum and adipose tissue along with RBP4 levels in serum samples of 236 participants of the GraMo adult cohort. Associations were explored by multivariable linear regression analyses and Weighted Quantile Sum regression. Polychlorinated biphenyls (PCBs) 180, 153 and 138 were related to decreased adipose tissue retinol levels and increased serum RBP4/retinol ratio. Dicofol concentrations > limit of detection were associated with decreased retinol levels in serum and adipose tissue. Additionally, increased adipose tissue retinol levels were linked to an attenuation in previously-reported associations of adipose tissue PCB-153 with in situ superoxide dismutase activity. Our results revealed a suggestive link between retinoids, PCBs and redox microenvironment, potentially relevant for both mechanistic and public health purposes.


Assuntos
Poluentes Ambientais , Bifenilos Policlorados , Tecido Adiposo/metabolismo , Adulto , Poluentes Ambientais/metabolismo , Poluentes Ambientais/toxicidade , Humanos , Oxirredução , Poluentes Orgânicos Persistentes , Bifenilos Policlorados/metabolismo , Bifenilos Policlorados/toxicidade , Retinoides/metabolismo , Proteínas Plasmáticas de Ligação ao Retinol/metabolismo
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