Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 5.125
Filtrar
1.
Chemosphere ; 310: 136751, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36209847

RESUMO

In the recent era, the increasing persistence of hazardous contaminants is badly affecting the globe in many ways. Due to high environmental contamination, almost every second species on earth facing the worst issue in their survival. Advances in newer remediation approaches may help enhance bioremediation's quality, while conventional procedures have failed to remove hazardous compounds from the environment. Chemical and physical waste cleanup approaches have been used in current circumstances; however, these methods are costly and harmful to the environment. Thus, there has been a rise in the use of bioremediation due to an increase in environmental contamination, which led to the development of genetically engineered microbes (GEMs). It is safer and more cost-effective to use engineered microorganisms rather than alternative methods. GEMs are created by introducing a stronger protein into bacteria through biotechnology or genetic engineering to enhance the desired trait. Biodegradation of oil spills, halobenzoates naphthalenes, toluenes, trichloroethylene, octanes, xylenes etc. has been accomplished using GEMs such bacteria, fungus, and algae. Biotechnologically induced microorganisms are more powerful than naturally occurring ones and may degrade contaminants faster because they can quickly adapt to new pollutants they encounter or co-metabolize. Genetic engineering is a worthy process that will benefit the environment and ultimately the health of our people.


Assuntos
Poluentes Ambientais , Recuperação e Remediação Ambiental , Humanos , Biodegradação Ambiental , Engenharia Genética , Poluentes Ambientais/metabolismo , Bactérias/genética , Bactérias/metabolismo
2.
J Hazard Mater ; 441: 129843, 2023 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-36113351

RESUMO

Cadmium (Cd) is the most widely distributed heavy metal pollutant in soil and has significant negative effects on crop yields and human health. Rhizobia can enhance soybean growth in the presence of heavy metals, and the legume-rhizobia symbiosis has been used to promote heavy-metal phytoremediation, but much remains to be learned about the molecular networks that underlie these effects. Here, we demonstrated that soybean root growth was strongly suppressed after seven days of Cd exposure but that the presence of rhizobia largely eliminated this effect, even prior to nodule development. Moreover, rhizobia did not appear to promote root growth by limiting plant Cd uptake: seedlings with and without rhizobia had similar root Cd concentrations. Previous studies have demonstrated a role for m6A RNA methylation in the response of rice and barley to Cd stress. We therefore performed transcriptome-wide m6A methylation profiling to investigate changes in the soybean RNA methylome in response to Cd with and without rhizobia. Here, we provide some of the first data on transcriptome-wide m6a RNA methylation patterns in soybean; m6A modifications were concentrated at the 3' UTR of transcripts and showed a positive relationship with transcript abundance. Transcriptome-wide m6A RNA methylation peaks increased in the presence of Cd, and the integration of m6A methylome and transcriptome results enabled us to identify 154 genes whose transcripts were both differentially methylated and differentially expressed in response to Cd stress. Annotation results suggested that these genes were associated with Ca2+ homeostasis, ROS pathways, polyamine metabolism, MAPK signaling, hormones, and biotic stress responses. There were 176 differentially methylated and expressed transcripts under Cd stress in the presence of rhizobia. In contrast to the Cd-only gene set, they were also enriched in genes related to auxin, jasmonic acid, and brassinosteroids, as well as abiotic stress tolerance. They contained fewer genes related to Ca2+ homeostasis and also included candidates with known functions in the legume-rhizobia symbiosis. These findings offer new insights into how rhizobia promote soybean root growth under Cd stress; they provide candidate genes for research on plant heavy metal responses and for the use of legumes in phytoremediation.


Assuntos
Poluentes Ambientais , Fabaceae , Metais Pesados , Rhizobium , Regiões 3' não Traduzidas , Brassinosteroides , Cádmio/metabolismo , Cádmio/toxicidade , Poluentes Ambientais/metabolismo , Epigenoma , Fabaceae/metabolismo , Hormônios/metabolismo , Humanos , Ácidos Indolacéticos , Metais Pesados/metabolismo , Metais Pesados/toxicidade , Poliaminas/metabolismo , RNA de Plantas/genética , Espécies Reativas de Oxigênio/metabolismo , Rhizobium/metabolismo , Solo , Soja/genética , Soja/metabolismo
3.
Sci Total Environ ; 855: 158888, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36165908

RESUMO

The union of Plant Growth-Promoting Bacteria (PGPB) and rhizosphere confers a series of functions beneficial to plant. However, the lack of an opearable in situ method limits the further understanding on the mechanism. In this study, a weak electric field was designed to regulate rhizospheric microflora in a constructed root-splitting reactor. Compared with the control, the aboveground and underground biomass of rice seedling increased by 17 % and 18 % (p < 0.05) respectively under the exist of weak electric field of 0.14 V/cm. The joint action of rhizosphere and PGPB displayed the detoxification ability in the condition of soluble petroleum hydrocarbons, where the height, stem diameter, biomass and root vigor of the plant was increased by 58 %, 32 %, 43 % and 48 % respectively than the control. The selective reproduction of endophytes and ectophytes (denitrifying, auxin-producing, hydrocarbon-degrading and electroactive bacteria) was observed under applied weak electric field, which enhanced the nitrogen utilization, cellular metabolic activity and resistance to toxic organics of plant. This was further confirmed by the up-regulated OTUs related to the hydrocarbon degradation function, tryptophan metabolism and metabolism of nicotinate and nicotinamide. Moreover, the weak electric field also enhanced the transfer ability of partial endophytes grown in the root to improve plant stress resistance. The results in this work inspired an exercisable method for in situ enrichment of PGPB in the rhizosphere to cope with food crisis and provided a new way to deal with sudden environmental events.


Assuntos
Poluentes Ambientais , Poluentes do Solo , Poluentes Ambientais/metabolismo , Raízes de Plantas/metabolismo , Rizosfera , Desenvolvimento Vegetal , Endófitos/metabolismo , Hidrocarbonetos/metabolismo , Bactérias/metabolismo , Plantas/metabolismo , Poluentes do Solo/análise , Biodegradação Ambiental , Microbiologia do Solo
4.
Environ Health Perspect ; 130(11): 117003, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36331819

RESUMO

BACKGROUND: Perfluorooctane sulfonate (PFOS) is a persistent environmental pollutant that has become a significant concern around the world. Exposure to PFOS may alter gut microbiota and liver metabolic homeostasis in mammals, thereby increasing the risk of cardiometabolic diseases. Diets high in soluble fibers can ameliorate metabolic disease risks. OBJECTIVES: We aimed to test the hypothesis that soluble fibers (inulin or pectin) could modulate the adverse metabolic effects of PFOS by affecting microbe-liver metabolism and interactions. METHODS: Male C57BL/6J mice were fed an isocaloric diet containing different fibers: a) inulin (soluble), b) pectin (soluble), or c) cellulose (control, insoluble). The mice were exposed to PFOS in drinking water (3µg/g per day) for 7 wk. Multi-omics was used to analyze mouse liver and cecum contents. RESULTS: In PFOS-exposed mice, the number of differentially expressed genes associated with atherogenesis and hepatic hyperlipidemia were lower in those that were fed soluble fiber than those fed insoluble fiber. Shotgun metagenomics showed that inulin and pectin protected against differences in microbiome community in PFOS-exposed vs. control mice. It was found that the plasma PFOS levels were lower in inulin-fed mice, and there was a trend of lower liver accumulation of PFOS in soluble fiber-fed mice compared with the control group. Soluble fiber intake ameliorated the effects of PFOS on host hepatic metabolism gene expression and cecal content microbiome structure. DISCUSSIONS: Results from metabolomic, lipidomic, and transcriptomic studies suggest that inulin- and pectin-fed mice were less susceptible to PFOS-induced liver metabolic disturbance, hepatic lipid accumulation, and transcriptional changes compared with control diet-fed mice. Our study advances the understanding of interaction between microbes and host under the influences of environmental pollutants and nutrients. The results provide new insights into the microbe-liver metabolic network and the protection against environmental pollutant-induced metabolic diseases by high-fiber diets. https://doi.org/10.1289/EHP11360.


Assuntos
Poluentes Ambientais , Inulina , Camundongos , Masculino , Animais , Inulina/metabolismo , Inulina/farmacologia , Lipidômica , Metagenômica , Transcriptoma , Camundongos Endogâmicos C57BL , Fígado , Poluentes Ambientais/metabolismo , Fibras na Dieta/metabolismo , Fibras na Dieta/farmacologia , Pectinas/metabolismo , Pectinas/farmacologia , Mamíferos
5.
Front Endocrinol (Lausanne) ; 13: 995503, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36339434

RESUMO

Thyroid disruptors are found in food, atmosphere, soil, and water. These contaminants interfere with the thyroid function through the impairment of thyroid hormone synthesis, plasma transport, peripheral metabolism, transport into the target cells, and thyroid hormone action. It is well known that iodide uptake mediated by the sodium-iodide symporter (NIS) is the first limiting step involved in thyroid hormones production. Therefore, it has been described that several thyroid disruptors interfere with the thyroid function through the regulation of NIS expression and/or activity. Perchlorate, nitrate, and thiocyanate competitively inhibit the NIS-mediated iodide uptake. These contaminants are mainly found in food, water and in the smoke of cigarettes. Although the impact of the human exposure to these anions is highly controversial, some studies indicated their deleterious effects in the thyroid function, especially in individuals living in iodine deficient areas. Considering the critical role of thyroid function and the production of thyroid hormones for growth, metabolism, and development, this review summarizes the impact of the exposure to these NIS-inhibitors on thyroid function and their consequences for human health.


Assuntos
Poluentes Ambientais , Percloratos , Humanos , Percloratos/toxicidade , Percloratos/metabolismo , Tiocianatos/metabolismo , Tiocianatos/farmacologia , Nitratos/metabolismo , Nitratos/farmacologia , Glândula Tireoide/metabolismo , Poluentes Ambientais/metabolismo , Iodetos/metabolismo , Iodetos/farmacologia , Hormônios Tireóideos , Água/metabolismo
6.
Ying Yong Sheng Tai Xue Bao ; 33(10): 2871-2880, 2022 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-36384625

RESUMO

Due to the impacts of refractory organic pollutants and environment on the water treatment system, the sewage quality can not reach the standard. It is an effective measure to improve the efficiency of wastewater treatment by introducing exogenous engineering strains with relevant functional genes and the ability of horizontal gene transfer. In sewage treatment system, there are bacteria secreting signal molecules with quorum sensing. When population density reaches induction threshold, the bacteria would activate the related genes expression (such as biofilm formation, bioluminescent, antibiotics synthesis and virulence factor expression, etc.) through releasing signaling molecules, and thus trigger the behavior of other groups. Previously, researches about quorum sensing mainly concentrated on signal transduction, microbial social behavior, and medical microbiology. In recent years, stu-dies found that quorum sensing plays an important role in wastewater biological treatment and affects the colonization of the microorganism strain and pollutants degradation. Therefore, the regulation of quorum-sensing behavior is the key factor in the bioaugmentation performance. Here, we review the signaling molecules mechanism, the release of signaling molecules and its influence factors, the colonization of microbial community and the removal of pollutants. We further discussed the research from the perspective of quorum sensing biological process. The aim was to provide new idea for the effective implementation of bioaugmentation technology and the improvement of wastewater treatment efficiency, and to provide a theoretical reference for the in-depth understanding of quorum sensing regulation behavior in the process of bioaugmentation.


Assuntos
Poluentes Ambientais , Purificação da Água , Percepção de Quorum/fisiologia , Esgotos , Bactérias/genética , Poluentes Ambientais/metabolismo
7.
Int J Mol Sci ; 23(20)2022 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-36293069

RESUMO

Experimental liver injury with hepatocelluar necrosis and abnormal liver tests is caused by exposure to heavy metals (HMs) like aluminum, arsenic, beryllium, cadmium, chromium, cobalt, copper, iron, lead, mercury, molybdenum, nickel, platinum, thallium, titanium, vanadium, and zinc. As pollutants, HMs disturb the ecosystem, and as these substances are toxic, they may affect the health of humans and animals. HMs are not biodegradable and may be deposited preferentially in the liver. The use of animal models can help identify molecular and mechanistic steps leading to the injury. HMs commonly initiate hepatocellular overproduction of ROS (reactive oxygen species) due to oxidative stress, resulting in covalent binding of radicals to macromolecular proteins or lipids existing in membranes of subcellular organelles. Liver injury is facilitated by iron via the Fenton reaction, providing ROS, and is triggered if protective antioxidant systems are exhausted. Ferroptosis syn pyroptosis was recently introduced as mechanistic concept in explanations of nickel (Ni) liver injury. NiCl2 causes increased iron deposition in the liver, upregulation of cyclooxygenase 2 (COX-2) protein and mRNA expression levels, downregulation of glutathione eroxidase 4 (GPX4), ferritin heavy chain 1 (FTH1), nuclear receptor coactivator 4 (NCOA4) protein, and mRNA expression levels. Nickel may cause hepatic injury through mitochondrial damage and ferroptosis, defined as mechanism of iron-dependent cell death, similar to glutamate-induced excitotoxicity but likely distinct from apoptosis, necrosis, and autophagy. Under discussion were additional mechanistic concepts of hepatocellular uptake and biliary excretion of mercury in exposed animals. For instance, the organic anion transporter 3 (Oat3) and the multidrug resistance-associated protein 2 (Mrp2) were involved in the hepatic handling of mercury. Mercury treatment modified the expression of Mrp2 and Oat3 as assessed by immunoblotting, partially explaining its impaired biliary excretion. Concomitantly, a decrease in Oat3 abundance in the hepatocyte plasma membranes was observed that limits the hepatic uptake of mercury ions. Most importantly and shown for the first time in liver injury caused by HMs, titanium changed the diversity of gut microbiota and modified their metabolic functions, leading to increased generation of lipopolysaccharides (LPS). As endotoxins, LPS may trigger and perpetuate the liver injury at the level of gut-liver. In sum, mechanistic and molecular steps of experimental liver injury due to HM administration are complex, with ROS as the key promotional compound. However, additional concepts such as iron used in the Fenton reaction, ferroptosis, modification of transporter systems, and endotoxins derived from diversity of intestinal bacteria at the gut-liver level merit further consideration.


Assuntos
Arsênio , Poluentes Ambientais , Mercúrio , Metais Pesados , Transportadores de Ânions Orgânicos , Humanos , Animais , Níquel/metabolismo , Zinco/metabolismo , Cobre/metabolismo , Cádmio/metabolismo , Cobalto/metabolismo , Vanádio/metabolismo , Molibdênio/metabolismo , Alumínio/metabolismo , Cromo/metabolismo , Arsênio/toxicidade , Arsênio/metabolismo , Titânio/metabolismo , Berílio/metabolismo , Ferro/metabolismo , Platina/metabolismo , Tálio/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Ciclo-Oxigenase 2/metabolismo , Mercúrio/toxicidade , Antioxidantes/metabolismo , Lipopolissacarídeos/metabolismo , Ecossistema , Apoferritinas/metabolismo , Metais Pesados/toxicidade , Metais Pesados/metabolismo , Fígado/metabolismo , Poluentes Ambientais/metabolismo , Glutationa/metabolismo , Necrose/metabolismo , Glutamatos/metabolismo , Coativadores de Receptor Nuclear , Transportadores de Ânions Orgânicos/metabolismo , RNA Mensageiro/metabolismo
8.
Chemosphere ; 309(Pt 2): 136666, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36220431

RESUMO

With the development of medical drugs, the widely used tetracycline has brought many adverse effects on the ecosystem and human health. Tetracycline pollution of water environment is becoming more and more serious, and has become an emerging environmental problem. As single celled organisms, microalgae are not only model organisms for risk assessment of aquatic ecosystems, but also can efficiently purify sewage. Microalgae-mediated pollutant remediation has attracted more and more attention from researchers. In this paper, Chlamydomonas reinhardtii (C. reinhardtii) was used to remove tetracycline in aqueous solution, and the removal efficiency and mechanism of microalgae on tetracycline were studied. The results showed that the removal rates of tetracycline by active and inactivated microalgae at a density of 5 × 106 cells·mL-1 were 81.9% and 89.8%, respectively. C. reinhardtii removed tetracycline through biosorption and nonmetabolic processes. Microalgal cell supernatant and hydroxyl radicals could significantly promote the removal of tetracycline. The positively charged tetracycline was electrostatically adsorbed on the microalgae surface and extracellular polymeric substances. Microalgae biomass can promote the production of ROS and enhance the ability of microalgae to remove tetracycline.


Assuntos
Chlamydomonas reinhardtii , Poluentes Ambientais , Microalgas , Humanos , Ecossistema , Esgotos , Espécies Reativas de Oxigênio/metabolismo , Tetraciclina/farmacologia , Microalgas/metabolismo , Biomassa , Antibacterianos/farmacologia , Água/metabolismo , Poluentes Ambientais/metabolismo , Águas Residuárias
9.
Environ Pollut ; 315: 120358, 2022 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-36228850

RESUMO

The susceptibility to trace metals and legacy POPs is different between terrestrial and marine mammals. In this study, we established the first cell line from Indo-Pacific finless porpoises and compared the cellular responses of skin fibroblast cells from Pygmy killer whales, Pantropic spotted dolphins, Indo-Pacific finless porpoises, mice, and humans following exposure to copper, methylmercury, cadmium, PCB126, PCB153, and BDE47 to better understand the interspecies sensitivities of mammals to chemical pollutants. We conducted a risk assessment by comparing no-observed effect concentrations (NOEC), lowest-observed effect concentrations (LOEC), and half maximal effective concentrations (EC50) from cell viability assays and previously reported pollutant body burdens in mammals. Based on the in vitro data, Indo-Pacific finless porpoises were more sensitive to copper and methylmercury than other mammals. PCB153 exposure reduced cell viability in all mammals except humans, while PCB126 was more potent, with 13.33 µg/mL exposure reducing cell viability in all mammals. In contrast, BDE47 exposure reduced cell viability only in terrestrial mammals in addition to pantropic spotted dolphin. Based on the in vitro data and the natural context of metal concentrations, both methylmercury and cadmium posed a higher risk to cetaceans than human, while copper posed a lower risk to cetaceans. All three legacy POPs (PCB126, PCB153, and BDE47) posed minor risk to cetaceans for short-term exposure. This study demonstrated that a species-specific in vitro model may provide more accurate information on the potential risk of pollutants to mammals. However, due to the bioamplification of POPs and their potential impact on the endocrine system and immune system of cetaceans, risk assessment with long-term exposure with more in vitro models should be further studied.


Assuntos
Golfinhos , Poluentes Ambientais , Compostos de Metilmercúrio , Toninhas , Oligoelementos , Poluentes Químicos da Água , Humanos , Animais , Camundongos , Poluentes Químicos da Água/análise , Compostos de Metilmercúrio/metabolismo , Cobre/toxicidade , Cobre/metabolismo , Cádmio/metabolismo , Toninhas/metabolismo , Golfinhos/metabolismo , Oligoelementos/toxicidade , Oligoelementos/metabolismo , Poluentes Ambientais/metabolismo , Fibroblastos
10.
Arch Immunol Ther Exp (Warsz) ; 70(1): 24, 2022 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-36181646

RESUMO

Humans are exposed to a number of environmental pollutants every day. Among them, endocrine disruptors are particularly harmful to human health. Bisphenol A (BPA) is a xenoestrogen that has been shown to disrupt the endocrine system and cause reproductive toxicity. In this study, we aimed to verify the potential relationship between BPA and miscarriage involving the formation of neutrophil extracellular traps (NETs). Blood samples were collected from healthy women and women who had miscarriage in the first trimester of pregnancy. The serum levels of cytoplasmic anti-PR3 antibody and perinuclear anti-MPO antibody were determined using an immunoenzymatic method. The concentrations of key proinflammatory proteins TNF-α and MCP-1, as well as NADPH oxidase subunits NOX1 and NCF2, were also measured in the serum samples. The serum concentration of BPA was determined using gas chromatography. The results showed that the concentrations of BPA were significantly elevated in the serum of women who had miscarriage compared to the control group, with the highest concentration found in the "NETs-positive" group. The levels of MCP-1 and TNF-α were significantly higher in the "NETs-positive" group compared to the "NETs-negative" and control group. The levels of NOX1 and NCF2 were also higher in the "NETs-positive" group compared to the "NETs-negative" group. The study showed that BPA could play a role in the course of miscarriage through the formation of NETs. The results indicate the need to limit the exposure of women planning pregnancy to xenoestrogens, including BPA.


Assuntos
Aborto Espontâneo , Disruptores Endócrinos , Poluentes Ambientais , Armadilhas Extracelulares , Compostos Benzidrílicos , Disruptores Endócrinos/metabolismo , Disruptores Endócrinos/farmacologia , Poluentes Ambientais/metabolismo , Poluentes Ambientais/farmacologia , Armadilhas Extracelulares/metabolismo , Feminino , Humanos , NADPH Oxidases/metabolismo , NADPH Oxidases/farmacologia , Fenóis , Gravidez , Fator de Necrose Tumoral alfa/metabolismo
11.
Environ Sci Technol ; 56(20): 14361-14374, 2022 10 18.
Artigo em Inglês | MEDLINE | ID: mdl-36197753

RESUMO

Marine environmental monitoring efforts often rely on the bioaccumulation of persistent anthropogenic contaminants in organisms to create a spatiotemporal record of the ecosystem. Intercorrelation results from the origin, uptake, and transport of these contaminants throughout the ecosystem and may be affected by organism-specific processes such as biotransformation. Here, we explore trends that machine learning tools reveal about a large, recently released environmental chemistry data set of common anthropogenic pollutants measured in the eggs of five seabird species from the North Pacific Ocean. We modeled these data with a variety of machine learning approaches and found models that could accurately determine a range of taxonomic and spatiotemporal trends. We illustrate a general workflow and set of analysis tools that can be used to identify interpretable models which perform nearly as well as state-of-the-art "black boxes." For example, we found shallow decision trees that could resolve genus with greater than 96% accuracy using as few as two analytes and a k-nearest neighbor classifier that could resolve species differences with more than 94% accuracy using only five analytes. The benefits of interpretability outweighed the marginally improved accuracy of more complex models. This demonstrates how machine learning may be used to discover rational, quantitative trends in these systems.


Assuntos
Ecossistema , Poluentes Ambientais , Animais , Aves/metabolismo , Quimiometria , Monitoramento Ambiental , Poluentes Ambientais/metabolismo , Aprendizado de Máquina , Oceano Pacífico
12.
Toxicology ; 482: 153356, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36283488

RESUMO

Dysfunction of immune regulation plays a crucial role in the pathogenesis of many immune disorders in the body. The underlying mechanism is still not completely understood. Environmental pollution contributes to immune de-regulation. 3-methyl-4-nitrophenol (MNP) is one of the major environmental pollutants. This study aims to investigate the role of MNP in compromising immune regulatory functions in the intestine. A food allergy (FA) mouse model was established using ovalbumin (OVA) as the specific antigen. The activities of regulatory T cells in the mouse intestine were evaluated by flow cytometry and enzyme-linked immunosorbent assay. We found that MNP reduced the CD4+ Foxp3+ Treg frequency, increased Th17 cells, and converted Tregs to Th17 cells in the intestine. MNP induced the expression of IL-6 in regulatory T cells (Tregs). Estrogen receptor (ER) mediated the effects of MNP on promoting IL-6 expression in Tregs. The IL-6 in synergy with transforming growth factor (TGF)-ß to convert Tregs to Th17 cells. The concomitant exposure of MNP and OVA induced FA like response in mice. Modulation of the ER-STAT3-IL-6 signal pathway attenuated mouse FA response. In summary, MNP, an environmental pollutant, acts as an immunoadjuvant for developing FA. By activation of the estrogen receptor, MNP induces Tregs to express IL-6. IL-6 in synergy with TGF-ß converts Tregs to Th17 cells.


Assuntos
Poluentes Ambientais , Linfócitos T Reguladores , Camundongos , Animais , Nitrofenóis/toxicidade , Nitrofenóis/metabolismo , Poluentes Ambientais/metabolismo , Receptores de Estrogênio/metabolismo , Interleucina-6/metabolismo , Células Th17 , Ovalbumina , Fator de Crescimento Transformador beta/metabolismo , Intestinos , Fatores de Transcrição Forkhead/metabolismo , Fatores de Transcrição Forkhead/farmacologia
13.
Ecotoxicol Environ Saf ; 245: 114098, 2022 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-36137422

RESUMO

Cadmium (Cd), the common environmental pollutant, primarily targets at renal proximal tubules and induces nephrotoxicity. Cellular senescence, a phenomenon of cell growth arrest and a characteristics of maladaptive cell self-repair, is associated with renal disease progression. However, whether and how Cd induces renal tubular cells premature senescence is unknown. In our study, we found that Cd induced kidney damage and dysfunctions, which correlated with exacerbated tubular cell senescence, evidenced by increased senescence-associated ß-galactosidase activity, the upregulated protein expression of p53 and p21Waf1/Cip1 proteins, and elevated expression and secretion of cytokines in human proximal tubular epithelial HK-2 cells in vitro and in Cd-treated mice in vivo. Moreover, a S-phase arrest and decrease in Edu positive rate were found in Cd-treated HK-2 cells. Mechanistically, Cd suppressed the expression and activity of Sirtuin-1 (SIRT1), an anti-senescence deacetylase, resulting in the accumulation of acetylated p53 and upregulation of p21Waf1/Cip1. Activation of SIRT1 significantly abolished Cd-induced premature senescence and S-phase arrest. Finally, silencing p21Waf1/Cip1 efficiently delayed premature senescence and recovered cell cycle progression. These findings indicate that Cd promotes tubular cells senescence and impairs tubular cells regeneration, resulting in kidney dysfunctions, which could be ameliorated by SIRT1 activation.


Assuntos
Poluentes Ambientais , Sirtuína 1 , Animais , Cádmio/metabolismo , Cádmio/toxicidade , Senescência Celular , Inibidor de Quinase Dependente de Ciclina p21/genética , Inibidor de Quinase Dependente de Ciclina p21/metabolismo , Citocinas/metabolismo , Poluentes Ambientais/metabolismo , Humanos , Rim/metabolismo , Camundongos , Sirtuína 1/genética , Sirtuína 1/metabolismo , Proteína Supressora de Tumor p53/genética , Proteína Supressora de Tumor p53/metabolismo , beta-Galactosidase
14.
Sci Total Environ ; 853: 158388, 2022 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-36049693

RESUMO

Microorganisms that can simultaneously remediate organic pollutants and heavy metal contamination are great significance in bioremediation. Nevertheless, reports of such microorganisms are still scarce. Here, Pseudomonas sp. YH-1 and Rhodococcus sp. YH-3 were isolated and identified, and they showed greater tolerance to hexavalent (VI) (750 and 800 mg·L-1). The constructed bacteria consortium YH (YH-1:YH-3 = 1:1) could simultaneously degrade 41.69% of pyrene (50 mg·L-1) and remove 76.67% of Cr(VI) (30 mg·L-1) within 5 days. The potential mechanism of Cr(VI) tolerance of YH was further explored by genomic and microscopic analysis. The results showed that YH responded to Cr(VI) stress mainly through efflux of Cr(VI) by chrA and copZ, chromate reduction, DNA-repaired proteases reduces ROS damage, and biosorption by carboxyl, hydroxyl, amino functional groups. Strains YH-1 and YH-3 also contained a variety of genes associated with resistance to other heavy metals, such as cadmium (czcBD), mercury (merAPTR), manganese (mntABC) and copper (copAC, cusABRF and pcoBD). Based on GC-MS and genomic analysis, pyrene was degraded via salicylic acid and phthalic acid pathways. Moreover, a great number of genes related to aromatic hydrocarbon catabolism were identified in the genomes of YH-1 and YH-3. These results confirmed the potential application of the bacteria consortium YH in the bioremediation of water and soil co-contaminated with PAHs-heavy metals.


Assuntos
Poluentes Ambientais , Mercúrio , Metais Pesados , Cromatos , Cádmio , Cobre , Manganês , Espécies Reativas de Oxigênio , Cromo/metabolismo , Biodegradação Ambiental , Metais Pesados/metabolismo , Pirenos , Bactérias/metabolismo , Solo , Poluentes Ambientais/metabolismo , Água , Peptídeo Hidrolases , Ácido Salicílico , DNA
15.
Sci Total Environ ; 853: 158630, 2022 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-36084783

RESUMO

Exoelectrogens possess extraordinary degradation ability to various pollutants through extracellular electron transfer (EET). Compared with extracellular electron release process, intracellular electron transfer network is not yet fully recognized. Especially, controversy remains regarding the role of CymA, an essential electron-transfer hub of Shewanella oneidensis MR-1, in EET process. In this study, we thoroughly surveyed the intracellular transfer strategies during EET through dye decolorization. Loss of CymA severely impaired the reduction ability of S. oneidensis MR-1 to methyl orange (MO), but hardly affected the decolorization of aniline blue (AB). Complement of cymA fully restored the MO decolorization ability of ΔcymA mutant. The contribution of CymA to extracellular decolorization was subjected to MO concentrations. The defect in the decolorization ability of ΔcymA mutant was not evident at low MO concentration, but severe at high MO concentration. Further investigation revealed that EET rate determined the significance of CymA in the extracellular bioremediation by S. oneidensis MR-1. Coupled with MO concentrations increasing from 15 to 120 mg/L, the initial electron transfer rates of S. oneidensis MR-1 increased accordingly from 2.69 × 104 to 11.21 × 104 electrons CFU-1 s-1, which led to a gradual increase of the dependencyCymA. Thus, we first revealed that extracellular degradation performance could feedback regulate the intracellular electron transfer process of S. oneidensis MR-1. This work is helpful to fully understand the complex EET process of exoelectrogens and facilitates the application of exoelectrogens in bioremediation of environmental pollutants.


Assuntos
Poluentes Ambientais , Shewanella , Elétrons , Poluentes Ambientais/metabolismo , Retroalimentação , Shewanella/metabolismo , Transporte de Elétrons
16.
Environ Sci Pollut Res Int ; 29(51): 76611-76625, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36166130

RESUMO

A variety of both small and large biologically intriguing compounds can be found abundantly in the marine environment. Researchers are particularly interested in marine bacteria because they can produce classes of bioactive secondary metabolites that are structurally diverse. The main secondary metabolites produced by marine bacteria are regarded as steroids, alkaloids, peptides, terpenoids, biopolymers, and polyketides. The global urbanization leads to the increased use of organic pollutants that are both persistent and toxic for humans, other life forms and tend to biomagnified in environment. The issue can be addressed, by using marine microbial biopolymers with ability for increased bioremediation. Amongst biopolymers, the exopolysaccharides (EPS) are the most prominent under adverse environmental stress conditions. The present review emphasizes the use of EPS as a bio-flocculent for wastewater treatment, as an adsorbent for the removal of textile dye and heavy metals from industrial effluents. The biofilm-forming ability of EPS helps with soil reclamation and reduces soil erosion. EPS are an obvious choice being environmentally friendly and cost-effective in processes for developing sustainable technology. However, a better understanding of EPS biosynthetic pathways and further developing novel sustainable technologies is desirable and certainly will pave the way for efficient usage of EPS for environment cleanup.


Assuntos
Poluentes Ambientais , Metais Pesados , Policetídeos , Humanos , Biodegradação Ambiental , Metais Pesados/metabolismo , Bactérias/metabolismo , Biopolímeros/química , Solo , Poluentes Ambientais/metabolismo , Terpenos/metabolismo
17.
Environ Pollut ; 312: 120084, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36057328

RESUMO

Research over the last three decades showed that chromium, particularly the oxyanion chromate Cr(VI) behaves as a toxic environmental pollutant that strongly damages plants due to oxidative stress, disruption of nutrient uptake, photosynthesis and metabolism, and ultimately, represses growth and development. However, mild Cr(VI) concentrations promote growth, induce adventitious root formation, reinforce the root cap, and produce twin roots from single root meristems under conditions that compromise cell viability, indicating its important role as a driver for root organogenesis. In recent years, considerable advance has been made towards deciphering the molecular mechanisms for root sensing of chromate, including the identification of regulatory proteins such as SOLITARY ROOT and MEDIATOR 18 that orchestrate the multilevel dynamics of the oxyanion. Cr(VI) decreases the expression of several glutamate receptors, whereas amino acids such as glutamate, cysteine and proline confer protection to plants from hexavalent chromium stress. The crosstalk between plant hormones, including auxin, ethylene, and jasmonic acid enables tissues to balance growth and defense under Cr(VI)-induced oxidative damage, which may be useful to better adapt crops to biotic and abiotic challenges. The highly contrasting responses of plants manifested at the transcriptional and translational levels depend on the concentration of chromate in the media, and fit well with the concept of hormesis, an adaptive mechanism that primes plants for resistance to environmental challenges, toxins or pollutants. Here, we review the contrasting facets of Cr(VI) in plants including the cellular, hormonal and molecular aspects that mechanistically separate its toxic effects from biostimulant outputs.


Assuntos
Cromatos , Poluentes Ambientais , Cromatos/metabolismo , Cromo/química , Cisteína/metabolismo , Cisteína/farmacologia , Poluentes Ambientais/metabolismo , Etilenos/metabolismo , Etilenos/farmacologia , Glutamatos/metabolismo , Glutamatos/farmacologia , Hormese , Ácidos Indolacéticos/metabolismo , Reguladores de Crescimento de Plantas/metabolismo , Raízes de Plantas/metabolismo , Plantas/metabolismo , Prolina/metabolismo , Prolina/farmacologia
18.
Ecotoxicol Environ Saf ; 244: 114055, 2022 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-36075122

RESUMO

Cadmium is a ubiquitous environmental pollutant, which can increase the risk of preeclampsia. This study was designed to determine the mechanism of cadmium exposure during pregnancy impaired placental angiogenesis that was associated with the occurrence of preeclampsia. The effects of cadmium exposure on placental thyroid hormone receptor signaling were explored. JEG3 cells were treated with CdCl2 (20 µM) and the Dio2 inhibitor, IOP (100 µM). Cadmium levels in maternal blood and placentae were increased in preeclampsia group. Placental angiogenesis of preeclampsia was decreased with decreased expression of PLGF and VEGF and increased expression of sFlt1. Meanwhile, the expression and nuclear translocation of thyroid hormone receptor α were decreased in preeclampsia placenta, as well as the expression of Dio2, but not the expression and nuclear translocation of thyroid hormone receptor ß. Furthermore, we found that cadmium exposure downregulated the expression of thyroid hormone receptor α and Dio2, but not the expression of thyroid hormone receptor ß in JEG3 cells. Also, we found that cadmium exposure decreased the expression of PLGF and VEGF and increased the expression of sFlt1 in JEG3 cells. IOP pretreatment decreased the expression of PLGF and increased the expression of sFlt1. In conclusion, our results elucidated that cadmium exposure would impair placental angiogenesis in preeclampsia through disturbing thyroid hormone receptor signaling.


Assuntos
Poluentes Ambientais , Pré-Eclâmpsia , Cádmio/metabolismo , Linhagem Celular Tumoral , Poluentes Ambientais/metabolismo , Feminino , Humanos , Neovascularização Patológica , Placenta/metabolismo , Fator de Crescimento Placentário/metabolismo , Fator de Crescimento Placentário/farmacologia , Pré-Eclâmpsia/induzido quimicamente , Pré-Eclâmpsia/metabolismo , Gravidez , Receptores dos Hormônios Tireóideos/metabolismo , Receptores alfa dos Hormônios Tireóideos/metabolismo , Receptores beta dos Hormônios Tireóideos/metabolismo , Fator A de Crescimento do Endotélio Vascular/genética , Fator A de Crescimento do Endotélio Vascular/metabolismo , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/metabolismo , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/farmacologia
19.
Chemosphere ; 308(Pt 3): 136474, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36126739

RESUMO

Selenium (Se) is a vital trace element for many living organisms inclusive of aquatic species. Although the antagonistic action of this element against other pollutants has been previously described for mammals and birds, limited information on the join effects in bivalves is available. To this end, bivalves of the species Scrobicularia plana were exposed to Se and Cd individually and jointly. Digestive glands were analysed to determine dose-dependent effects, the potential influence of Se on Cd bioaccumulationas well as the possible recover of the oxidative stress and metabolic alterations induced by Cd. Selenium co-exposure decreased the accumulation of Cd at low concentrations. Cd exposure significantly altered the metabolome of clams such as aminoacyltRNA biosynthesis, glycerophospholipid and amino acid metabolism, while Se co-exposure ameliorated several altered metabolites such asLysoPC (14:0), LysoPE (20:4), LysoPE (22:6), PE (14:0/18:0), PE (20:3/18:4) andpropionyl-l-carnitine.Additionally, Se seems to be able to regulate the redox status of the digestive gland of clams preventing the induction of oxidativedamage in this organ. This study shows the potential Se antagonism against Cd toxicity in S. plana and the importance to study join effects of pollutants to understand the mechanism underlined the effects.


Assuntos
Bivalves , Poluentes Ambientais , Selênio , Oligoelementos , Aminoácidos/metabolismo , Animais , Bioacumulação , Bivalves/metabolismo , Cádmio/metabolismo , Carnitina/metabolismo , Carnitina/farmacologia , Poluentes Ambientais/metabolismo , Glicerofosfolipídeos/metabolismo , Mamíferos/metabolismo , Estresse Oxidativo , Selênio/metabolismo , Selênio/toxicidade , Oligoelementos/metabolismo
20.
Chemosphere ; 308(Pt 3): 136580, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36155011

RESUMO

Although chlordane-related compounds (CHLs) have been regulated, a variety of CHLs are still identified and detected in wild birds and eggs. Embryo is one of fragile periods and is very susceptible to toxic effects of pollutants. In this study, the fate of CHLs during embryo development and degradation of CHLs in neonatal chick were investigated. During embryo development, CHLs were mainly distributed to the liver and muscle, in which trans-nonachlor and an octachlorochlordane (MC5) were hardly metabolized and showed the high persistence, implying a greater risk to birds' offspring. CHLs with the lower Kow were found to be higher uptake efficiency in embryo, implying contaminants with the lower lipophilicity may contribute to their transport to embryo. Furthermore, the effects of CHLs on the metabolome of neonatal chicks was evaluated. The ether lipid metabolism and glycerophospholipid metabolism were found to be significantly affected, which may disturb the angiogenesis and endothelial cell migration in embryogenesis. Taken together, the lipophilicity of contaminants might be a main factor influencing their transport to embryo, and metabolomics results improve understanding of the effects of CHLs on embryo.


Assuntos
Clordano , Poluentes Ambientais , Animais , Bioacumulação , Embrião de Galinha , Galinhas/metabolismo , Clordano/análise , Poluentes Ambientais/metabolismo , Poluentes Ambientais/toxicidade , Éteres , Glicerofosfolipídeos/metabolismo , Metabolômica
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...