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1.
Prog Chem Org Nat Prod ; 109: 415-450, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31637531

RESUMO

Natural remedies have for centuries played a significant role in traditional medicine and continue to be a unique reservoir of new chemical entities in drug discovery and development research. Propolis is a natural substance, collected by bees mainly from plant resins, which has a long history of use as a folk remedy to treat a variety of ailments. The highly variable phytochemical composition of propolis is attributed to differences in plant diversity within the geographic regions from which it is collected. Despite the fact that the last five decades has seen significant advancements in the understanding of the chemistry and biological activity of propolis, a search of the literature has revealed that studies on African propolis to date are rather limited. The aim of this contribution is to report on the current body of knowledge of African propolis, with a particular emphasis on its chemistry and biological activity. As Africa is a continent with a rich flora and a vast diversity of ecosystems, there is a wide range of propolis phytochemicals that may be exploited in the development of new drug scaffolds.


Assuntos
Própole/química , África , Animais , Abelhas , Compostos Fitoquímicos/análise , Resinas Vegetais/química
2.
An Acad Bras Cienc ; 91(3): e20180646, 2019 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-31411259

RESUMO

The hepatoprotective effects of the ethanolic extracts of propolis (EEP) on alcohol-induced liver steatosis were investigated in Wistar rats. Chronic alcoholic fatty liver was induced by administration of 52% alcohol to male Wistar rats at the dose of 1% body weight for 7 weeks. Then animals were simultaneously treated with 50% ethanol solutions of EEP or normal saline at the dose of 0.1% body weight for 4 further weeks. Serological analyses and liver histopathology studies were performed to investigate the development of steatosis. Microarray analysis was conducted to investigate the alterations of hepatic gene expression profiling. Our results showed that 4-week treatment of EEP helped to restore the levels of various blood indices, liver function enzymes and the histopathology of liver tissue to normal levels. Results from the microarray analysis revealed that the hepatic expressions of genes involved in lipogenesis were significantly down-regulated by EEP treatment, while the transcriptional expressions of functional genes participating in fatty acids oxidation were markedly increased. The ability of EEP to reduce the negative effects of alcohol on liver makes propolis a potential natural product for the alternative treatment of alcoholic fatty liver.


Assuntos
Fígado Gorduroso Alcoólico/metabolismo , Hepatopatias Alcoólicas/metabolismo , Extratos Vegetais/metabolismo , Própole/metabolismo , Substâncias Protetoras/metabolismo , Alanina Transaminase/metabolismo , Animais , Apiterapia/métodos , Aspartato Aminotransferases/metabolismo , Colesterol/metabolismo , Modelos Animais de Doenças , Etanol , Ácidos Graxos/biossíntese , Fígado Gorduroso Alcoólico/tratamento farmacológico , Fígado Gorduroso Alcoólico/genética , Fígado Gorduroso Alcoólico/patologia , Hepatopatias Alcoólicas/tratamento farmacológico , Hepatopatias Alcoólicas/genética , Hepatopatias Alcoólicas/patologia , Masculino , Oxirredução , Extratos Vegetais/química , Extratos Vegetais/uso terapêutico , Própole/química , Própole/uso terapêutico , Substâncias Protetoras/química , Substâncias Protetoras/uso terapêutico , Ratos Wistar , Análise Serial de Tecidos/métodos , Transcrição Genética/genética , Triglicerídeos/metabolismo
3.
Food Chem ; 298: 125080, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31260985

RESUMO

Propolis extract was investigated as potential substitute for sorbate in orangeade. Extract was prepared by using aqueous solution of hydroxypropyl-beta-cyclodextrins. Propolis extract was incorporated in non-carbonated orange soft drinks and its antioxidant activity, microbiological stability and color changes were estimated and compared to those of orangeade containing potassium sorbate. l-Ascorbic acid (AsA) degradation at concentrations 0.13 and 1.3% w/w was investigated in the presence of propolis during storage using High Performance Liquid Chromatography-Ion Exclusion Column (HPLC-IEC). The results indicate that the rate of degradation decreased with an increase in ascorbic acid concentration, while addition of propolis affected the degradation rate of samples containing a high AsA concentration. The antifungal effect of propolis extract, potassium sorbate and their combination was assayed. Results showed the inhibition of Aspergillus spp. and B. bruxellensis inhibited in low combined concentrations antimicrobials, while Aspergillus spp. and T. macrosporus were inhibited at 450 mg/g propolis extract.


Assuntos
Bebidas , Conservação de Alimentos/métodos , Conservantes de Alimentos/química , Própole/química , Anti-Infecciosos , Antifúngicos/farmacologia , Antioxidantes/química , Ácido Ascórbico/análise , Ácido Ascórbico/química , Aspergillus/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão/métodos , Cor , Microbiologia de Alimentos , Extratos Vegetais/química , Própole/farmacologia , Ácido Sórbico/farmacologia
4.
An Acad Bras Cienc ; 91(2): e20180598, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31271566

RESUMO

We have established how natural compounds from green propolis collected by the species Apis mellifera act against the growth of Pythium aphanidermatum. On the basis of mass spectrometry (Q-ToF MS), we determined that Artepillin C, the major constituent of green propolis, underlies the effect and displays activity against P. aphanidermatum at a minimal inhibitory concentration of 750 µg.mL-1. Biophysical studies based on model membranes showed that this inhibitory effect may be linked with a membrane-related phenomenon: Artepillin C increases the permeability of membranes with relatively high fluidity in their lateral structure, a feature that is in line with the lipid composition reported for the cytoplasmic membrane of P. aphanidermatum. Therefore, the present study supports the use of the effective and inexpensive green propolis to control the impact of the dangerous phytopathogen P. aphanidermatum on agriculture.


Assuntos
Antifúngicos/farmacologia , Fenilpropionatos/farmacologia , Própole/química , Pythium/efeitos dos fármacos , Animais , Antifúngicos/isolamento & purificação , Abelhas , Espectrometria de Massas , Testes de Sensibilidade Microbiana , Fenilpropionatos/isolamento & purificação
5.
Chem Biodivers ; 16(7): e1900189, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31222938

RESUMO

The aim of this study was to evaluate the ethanolic extract of propolis originated from northern Turkey for its antiproliferative, apoptotic and cell cycle arrest promoting effects on MCF7, HGC27, A549 cancer cell lines and a healthy cell line (HUVEC) in terms of DNA content, morphological features, expression of cell cycle checkpoint proteins p21, p53, Cyclin D1 and immune checkpoint protein PD-L1. The extract showed moderate antiproliferative activity against all tested cancer cell lines with IC50 values in the range of 58.6-90.7 µg/mL in MTS assay. Further studies indicated that propolis extract exerted apoptotic effect on cancer cell lines, promoted cell cycle arrest through activation of p21 and resulted in accumulation at G0/G1 phase of cancer cells. Propolis treatment caused increased cell size, according to fluorescent imaging except for MCF7. HPTLC analysis revealed that 3-O-methylquercetin, chrysin, caffeic acid, CAPE, galangin and pinocembrin were the main components of the extract. The amounts of caffeic acid and CAPE in the extract were found to be 5.5 and 11.1 mg/g, respectively, by a validated HPLC method. Our study is the first one, revealing effect of propolis on PD-L1 expression on certain cancer cell lines.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Extratos Vegetais/farmacologia , Própole/química , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Ciclo Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Relação Estrutura-Atividade , Turquia
6.
Chemosphere ; 232: 471-480, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31163323

RESUMO

Pesticide residues in beebread, live and dead honey bees, together with honey bee death rate were monitored from June 2016 to June 2018 in three apiaries, located near agricultural settings and in wildlands. Dead honey bees were only collected and analyzed when significant mortality episodes occurred and pesticide content in beeswax of each experimental apiary was evaluated at the beginning of the study. Samples were extracted by a modified QuEChERS procedure and screened for pesticides residues by liquid chromatography mass spectrometry (LC-MS/MS). Pesticide hazard in the samples was evaluated through the hazard quotient approach (HQ). Beebread was widely contaminated with coumaphos and amitraz degradate 2, 4-dimethylphenylformamide (DMF), miticides detected in 94 and 97% of samples respectively. However, insecticides sprayed during citrus bloom like chlorpyrifos (up to 167 ng g -1) and dimethoate (up to 34 ng g -1) were the main responsible of the relevant pesticide hazard in this matrix. Pesticide levels in live bees were mostly residual, and pesticide hazard was low. Beeswax of the apiaries, contaminated by miticides, revealed a low pesticide hazard to honey bee colonies. Acute mortality episodes occurred only in the two apiaries located near agricultural settings. Dead bees collected during these episodes revealed high levels (up to 2700 ng g -1) of chlorpyrifos, dimethoate, omethoate and imidacloprid. HQ calculated in dead bees exceeded up to 37 times the threshold value considered as elevated hazard to honey bee health.


Assuntos
Abelhas/efeitos dos fármacos , Monitoramento Ambiental/métodos , Poluentes Ambientais/análise , Inseticidas/análise , Resíduos de Praguicidas/análise , Acaricidas/análise , Acaricidas/envenenamento , Agricultura , Animais , Abelhas/química , Abelhas/crescimento & desenvolvimento , Monitoramento Ambiental/instrumentação , Poluentes Ambientais/toxicidade , Inseticidas/toxicidade , Resíduos de Praguicidas/toxicidade , Própole/química , Espanha , Análise de Sobrevida , Ceras/química
7.
Molecules ; 24(9)2019 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-31075821

RESUMO

Brazilian green propolis is a complex mixture of natural compounds that is difficult to analyze and standardize; as a result, controlling its quality is challenging. In this study, we used the positive and negative modes of ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time of flight mass spectrometry in conjunction with high-performance liquid chromatography for the identification and characterization of seven phenolic acid compounds in Brazilian green propolis. The optimal operating conditions for the electrospray ionization source were capillary voltage of 3500 V and drying and sheath gas temperatures of 320 °C and 350 °C, respectively. Drying and sheath gas flows were set to 8 L/min and 11 L/min, respectively. Brazilian green propolis was separated using the HPLC method, with chromatograms for samples and standards measured at 310 nm. UPLC-ESI-QTOF-MS was used to identify the following phenolic compounds: Chlorogenic acid, caffeic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, caffeic acid phenethyl ester (CAPE), and artepillin C. Using a methodologically validated HPLC method, the seven identified phenolic acids were then quantified among different Brazilian green propolis. Results indicated that there were no significant differences in the content of a given phenolic acid across different Brazilian green propolis samples, owing to the same plant resin sources for each sample. Isochlorogenic acid B had the lowest content (0.08 ± 0.04) across all tested Brazilian green propolis samples, while the artepillin C levels were the highest (2.48 ± 0.94). The total phenolic acid content across Brazilian green propolis samples ranged from 2.14-9.32%. Notably, artepillin C quantification is an important factor in determining the quality index of Brazilian green propolis; importantly, it has potential as a chemical marker for the development of better quality control methods for Brazilian green propolis.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Hidroxibenzoatos/análise , Própole/química , Espectrometria de Massas por Ionização por Electrospray/métodos , Análise de Variância , Reprodutibilidade dos Testes
8.
Food Chem Toxicol ; 130: 99-108, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31112706

RESUMO

The aim of the present study was to investigate the detoxifying effects of aloe polysaccharide (APS), propolis, and the mixture of APS and propolis on the urinary excretion of major human tobacco carcinogens, BaP and an addictive stimulant alkaloid, nicotine. Smokers (≥20 cigarettes/day) were randomly classified into four subgroups (10 people/group) and were given 600 mg/day of APS, 600 mg/day of propolis, or 600 mg/day of the mixture of APS (420 mg/day) and propolis (180 mg/day) for four weeks. Urinary excretion of BaP and cotinine (a metabolite of nicotine) increased in a time-dependent manner increased after supplementation with APS (BaP, 2.23-fold; cotinine, 2.64-fold), propolis (BaP, 1.30-fold; cotinine, 2.08-fold), and the mixture (BaP, 2.33-fold; cotinine, 2.28-fold) compared with smoker control. Creatinine, glucose, and total bilirubin levels significantly decreased in a time-dependent manner after supplementation with APS (creatinine, 15.24%; glucose, 40.22%; total bilirubin, 48.82%), propolis (creatinine, 16.83%; glucose, 36.25%; total bilirubin, 52.59%), and the mixture (creatinine, 16.36%; glucose, 46.37%; total bilirubin, 39.20%) (p < 0.05). These results suggest that supplementation with APS, propolis, or the mixture could reduce the risk of cancer or other diseases associated with tobacco smoking by enhancing urinary excretion of BaP and nicotine.


Assuntos
Aloe/química , Cotinina/urina , Nicotina/metabolismo , Polissacarídeos/farmacologia , Própole/farmacologia , Fumar/urina , Adulto , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Nicotina/farmacocinética , Nicotina/urina , Polissacarídeos/administração & dosagem , Polissacarídeos/química , Própole/administração & dosagem , Própole/química , Adulto Jovem
9.
Carbohydr Polym ; 216: 25-35, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-31047065

RESUMO

The unique physicochemical and functional characteristics of starch-based biomaterials and wound dressings have been proposed for several biomedical applications. Film dressings of cornstarch/hyaluronic acid/ ethanolic extract of propolis (CS/HA/EEP) were prepared by solvent-casting and characterized by attenuated total reflectance/Fourier transform infrared spectroscopy, scanning electron microscopy, gas chromatography/mass spectrometry, light transmission, opacity measurements, EEP release, equilibrium swelling, and in vitro and in vivo evaluations. The CS/HA/0.5%EEP film dressing exhibited higher antibacterial activity against Staphylococcus aureus (2.08 ± 0.14 mm), Escherichia coli (2.64 ± 0.18 mm), and Staphylococcus epidermidis (1.02 ± 0.15 mm) in comparison with the CS, CS/HA, and CS/HA/0.25%EEP films. Also, it showed no cytotoxicity for the L929 fibroblast cells. This wound dressing could effectively accelerate the wound healing process at Wistar rats' skin excisions. These results indicate that enrichment of cornstarch wound dressings with HA and EEP can significantly enhance their potential efficacy as wound dressing material.


Assuntos
Antibacterianos/farmacologia , Ácido Hialurônico/química , Curativos Oclusivos , Própole/farmacologia , Amido/química , Animais , Antibacterianos/química , Antibacterianos/toxicidade , Galinhas , Escherichia coli/efeitos dos fármacos , Feminino , Fibroblastos/efeitos dos fármacos , Ácido Hialurônico/toxicidade , Hidrólise , Muramidase/química , Própole/química , Própole/toxicidade , Ratos Wistar , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus epidermidis/efeitos dos fármacos , Amido/toxicidade , Cicatrização/efeitos dos fármacos
10.
Molecules ; 24(9)2019 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-31058881

RESUMO

The principal objective of this study was to determine the anti-staphylococcal potential of ethanol extracts of propolis (EEPs). A total of 20 samples of propolis collected from apiaries located in different regions of Poland were used in the study. The two-fold broth microdilution method revealed some important differences in the antimicrobial activity of investigated EEPs. Up to the concentration of 4096 µg/mL no activity was observed against Gram-negative bacteria (E. coli and P. aeruginosa). Staphylococci exhibited much higher susceptibility. The highest efficiency observed for EEP12 and EEP20 (MIC values ranged between 32 and 256 µg/mL). However, the achievement of bactericidal effect usually required higher concentrations. In the case of clinical isolates of S. aureus MBC values for EEP12 and EEP20 ranged from 512 to 1024 µg/mL. The HPLC analysis revealed that these two products contained a higher concentration of flavonoids (flavonols, flavones, and flavanones) compared to other investigated EEPs. In checkerboard test, a synergistic anti-staphylococcal effect was observed for the action of EEP20 in combination with amikacin, kanamycin, gentamycin, tetracycline, and fusidic acid (all these antibiotics inhibit protein synthesis). Moreover, the investigated EEPs effectively eradicated staphylococcal biofilm. The obtained results clearly confirm the high anti-staphylococcal potential of propolis harvested in Polish apiaries.


Assuntos
Antibacterianos/farmacologia , Etanol/farmacologia , Própole/química , Antibacterianos/química , Antibacterianos/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Sinergismo Farmacológico , Etanol/química , Etanol/isolamento & purificação , Flavonoides/isolamento & purificação , Flavonoides/farmacologia , Testes de Sensibilidade Microbiana , Polônia , Staphylococcus/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos
11.
Chem Biodivers ; 16(7): e1900094, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31099458

RESUMO

This review updates the information upon the chemical composition of propolis from all Mediterranean countries as well as their biological properties and applications. The non-volatile fraction of propolis was characterized by the presence of phenolic acids and their esters and flavonoids. Nevertheless, in some countries, diterpenes were also present: Sicily (Italy), Croatia, Malta, Creta (Greece), Turkey, Cyprus, Egypt, Libya, Algeria and Morocco. The volatile fraction of propolis was characterized by the presence of benzoic acid and its esters, mono- and sesquiterpenes, being the oxygenated sesquiterpene ß-eudesmol characteristic of poplar propolis, whereas the hydrocarbon monoterpene α-pinene has been related with the presence of conifers. Regardless the chemical composition, there are common biological properties attributed to propolis. Owing to these attributes, propolis has been target of study for applications in diverse areas, such as food, medicine and livestock.


Assuntos
Própole/farmacologia , Argélia , Chipre , Egito , Grécia , Itália , Líbia , Marrocos , Própole/química , Própole/isolamento & purificação , Turquia
12.
Naturwissenschaften ; 106(5-6): 25, 2019 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-31069518

RESUMO

The main chemical composition of Sonoran propolis (SP), as well as its antiproliferative activity on cancer cells through apoptosis induction, has been reported. The chemical constitution of SP remained qualitatively similar throughout the year, whereas the antiproliferative effect on cancer cells exhibited significant differences amongst seasonal samples. The main goal of this study was to provide phytochemical and pharmacological evidence for the botanical source of SP and its antiproliferative constituents. A chemical comparative analysis of SP and plant resins of species found in the surrounding areas of the beehives was carried out by HPLC-UV-DAD, as well as by 1H NMR experiments. The antiproliferative activity on cancerous (M12.C3.F6, HeLa, A549, PC-3) and normal cell lines (L-929; ARPE-19) was assessed through MTT assays. Here, the main polyphenolic profile of SP resulted to be qualitatively similar to Populus fremontii resins (PFR). However, the antiproliferative activity of PFR on cancer cells did not consistently match that exhibited by SP throughout the year. Additionally, SP induced morphological modifications on treated cells characterised by elongation, similar to those induced by colchicine, and different to those observed with PFR treatment. These results suggest that P. fremontii is the main botanical source of SP along the year. Nevertheless, the antiproliferative constituents of SP that induce that characteristic morphological elongation on treated cells are not obtained from PFR. Moreover, the presence of kaempferol-3-methyl-ether in SP could point Ambrosia ambrosioides as a secondary plant source. In conclusion, SP is a bioactive poplar-type propolis from semi-arid zones, in which chemical compounds derived from other semi-arid plant sources than poplar contribute to its antiproliferative activity.


Assuntos
Própole/química , Própole/farmacologia , Células A549 , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Clima Desértico , Células HeLa , Humanos , Populus/química
13.
Molecules ; 24(7)2019 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-30965600

RESUMO

Propolis is a bee product with a wide range of biological activities and its chemical compounds depend highly on the type of plant accessible to the bees. The Changbai Mountains are a major mountain range in Northeast China and are one of the major bee product-producing areas in China. In this study, we evaluated the total phenolic acids and flavonoid contents as well as the antioxidant activity of propolis sampled from the Changbai Mountains area (CBM). We identified the major compounds and qualified their contents by HPLC-ESI/MS and HPLC-UV, and found that the content of p-coumaric acid and an unknown peak (CBE) in CBM propolis was higher than in propolis from other parts of China. The unknown compound CBE was isolated, purified, and identified as benzyl p-coumarate by MS and NMR. Possible plant sources of CBM propolis are Populus davidiana dode and Populus simonii Carr, which widely distributed in the Changbai Mountains area. CBM propolis is a new propolis type, that could be an excellent raw material for health foods and pharmaceuticals.


Assuntos
Antioxidantes/isolamento & purificação , Flavonoides/isolamento & purificação , Hidroxibenzoatos/isolamento & purificação , Própole/química , Animais , Antioxidantes/química , Antioxidantes/farmacologia , Abelhas , China , Cromatografia Líquida de Alta Pressão , Flavonoides/química , Flavonoides/farmacologia , Hidroxibenzoatos/química , Hidroxibenzoatos/farmacologia , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Populus/química , Populus/classificação , Propionatos/isolamento & purificação , Propionatos/farmacologia
14.
Exp Parasitol ; 200: 37-41, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30928354

RESUMO

Toxocara spp. are responsible for causing toxocariasis, a zoonotic disease of global importance, which is difficult to treat as the available drugs have moderate efficacy in the clinical resolution of the disease. A promising alternative to the existing drugs is Propolis, which is known for having biological and pharmacological properties such as antiparasitic, antioxidant, and antitumor activities. In this study, we report the in vitro anthelmintic activity of essential oil from Brazilian Red Propolis (EOP) against larvae of Toxocara cati. Approximately 100 larvae per well were cultivated in microplates containing RPMI-1640 medium and incubated in the presence of EOP (18.75, 37.5, 75, 150, 300 and 600 µg/mL) to determine the Minimum Inhibitory Concentration (MIC) and IC50 (concentration required to inhibit 50% of the population) values. Then, T. cati larvae treated with the MIC of EOP were inoculated in mice to evaluate their progression in vivo. A concentration of 600 µg/mL of EOP showed 100% larvicidal activity after exposure for 48 h, while 300 µg/mL represented the IC50 and CC50. The anthelmintic activity of EOP was confirmed by the inability of the treated T. cati larvae to infect the mice. Our findings demonstrate the potential of EOP as an anthelmintic.


Assuntos
Anti-Helmínticos/farmacologia , Óleos Voláteis/farmacologia , Própole/química , Toxocara/efeitos dos fármacos , Animais , Anti-Helmínticos/isolamento & purificação , Anti-Helmínticos/toxicidade , Células CHO , Corantes , Cricetinae , Cricetulus , Feminino , Concentração Inibidora 50 , Cinética , Larva/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos BALB C , Testes de Sensibilidade Microbiana , Movimento/efeitos dos fármacos , Óleos Voláteis/isolamento & purificação , Óleos Voláteis/toxicidade , Toxocara/fisiologia , Azul Tripano
15.
Biomed Res Int ; 2019: 7817063, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30915357

RESUMO

The aim of the study was to evaluate the effect of propylene film coated with solution of chitosan (CH), ethanolic extracts of propolis (EEP), and bee pollen (EEBP) and its combination on L. monocytogenes number in wrapped salmon, salami, and cheese. Sterile fragments of propylene film were coated with solution containing CH, CH+EEP, CH+EEBP, and CH+EEP+EEBP. The coated film was applied directly after preparation (AP) after 10 days of storage from preparation (AS). L. monocytogenes strains isolated from cheese, salmon, and salami were transferred on adequate food type. ATCC 19111 reference strain was placed on all examined slices. Contaminated slices were wrapped in the coated film. The film adhered strictly to the slices surface and was left for 0, 1, 6, 12, and 24 hours. Antilisterial activity of AP film was additionally assessed during 15-day storage of products wrapped in the coated film. In conclusion, the chitosan-coated film exhibited antibacterial activity. Incorporation of EPP and EEBP enhanced this activity. The antilisterial activity depended on the type and concentration of solutions, the types of food, and the origin of strains. This study proved that the time that passed since the use of coated film for packing food was of great importance.


Assuntos
Antibacterianos/química , Quitosana/química , Embalagem de Alimentos , Listeria monocytogenes/crescimento & desenvolvimento , Membranas Artificiais , Pólen/química , Polipropilenos/química , Própole/química , Animais , Abelhas , Microbiologia de Alimentos
16.
Molecules ; 24(6)2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30884752

RESUMO

Twelve propolis samples from different parts of Libya were investigated for their phytochemical constituents. Ethanol extracts of the samples and some purified compounds were tested against Trypanosoma brucei, Plasmodium falciparum and against two helminth species, Trichinella spiralis and Caenorhabditis elegans, showing various degrees of activity. Fourteen compounds were isolated from the propolis samples, including a novel compound Taxifolin-3-acetyl-4'-methyl ether (4), a flavanonol derivative. The crude extracts showed moderate activity against T. spiralis and C. elegans, while the purified compounds had low activity against P. falciparum. Anti-trypanosomal activity (EC50 = 0.7 µg/mL) was exhibited by a fraction containing a cardol identified as bilobol (10) and this fraction had no effect on Human Foreskin Fibroblasts (HFF), even at 2.0 mg/mL, thus demonstrating excellent selectivity. A metabolomics study was used to explore the mechanism of action of the fraction and it revealed significant disturbances in trypanosomal phospholipid metabolism, especially the formation of choline phospholipids. We conclude that a potent and highly selective new trypanocide may be present in the fraction.


Assuntos
Antiprotozoários/química , Proliferação de Células/efeitos dos fármacos , Própole/química , Trypanosoma brucei brucei/efeitos dos fármacos , Animais , Antiprotozoários/farmacologia , Caenorhabditis elegans/efeitos dos fármacos , Caenorhabditis elegans/patogenicidade , Linhagem Celular , Fibroblastos/efeitos dos fármacos , Humanos , Líbia , Metabolômica , Plasmodium falciparum/efeitos dos fármacos , Plasmodium falciparum/patogenicidade , Polifenóis/química , Polifenóis/farmacologia , Própole/farmacologia , Trichinella spiralis/efeitos dos fármacos , Trichinella spiralis/patogenicidade , Trypanosoma brucei brucei/patogenicidade
17.
Int J Biol Macromol ; 131: 980-988, 2019 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-30910673

RESUMO

Our aim was to evaluate effects of natural rubber membrane (NR) associated with aqueous propolis extract (P) on the wound healing in rats burn model. The membranes were characterized for wettability and pore presence after the membrane preparation by casting the liquid solution in Petri dishes, at different temperatures (27 and 60 °C). Male Wistar rats were divided into treatment groups (n = 5 per group: control without treatment, silver sulfadiazine, NR, NRP) initiated immediately after second-degree burn and maintained during 10 days. The NRP membranes presented hydrophilic and porous characteristics. Macroscopic analysis from lesions showed that all groups presented crust formation. At the end of the experiment, the lesions treated with NRP membranes prepared at 60 °C had a higher regression percentage indicating a faster wound healing. The biopsies showed that the treatment with the NRP membranes induced a wound healing with collagen production, angiogenesis, reepithelization, and a small number of inflammatory cells, characterized by the crust detachment and the epithelium formation. In conclusion, NRP membranes are promising as a dressing for the burns treatment since were able to accelerate the healing process and tissue repair without the curative switch.


Assuntos
Materiais Biocompatíveis , Queimaduras/terapia , Membranas Artificiais , Própole , Borracha , Cicatrização , Animais , Materiais Biocompatíveis/química , Biomarcadores , Biópsia , Queimaduras/patologia , Interações Hidrofóbicas e Hidrofílicas , Própole/química , Ratos , Borracha/química
18.
Food Chem ; 287: 61-67, 2019 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-30857719

RESUMO

Propolis has demonstrated potential use as food preservative but it presents strong and unpleasant flavor that alters the sensory characteristics foods. A nanoemulsion was proposed to carry the Brazilian propolis extracts for use as natural food preservative. Antimicrobial and antioxidant activities and chemical constituents of the extracts were investigated. The latter were made by sequential extraction using different solvents (hexane, ethyl acetate and ethanol). Antimicrobial activity was evaluated by agar diffusion and microdilution methods and antioxidant activity by DPPH and ABTS assays. Extracts showed antibacterial and antioxidant activity, highlighting the ethanolic which contained artepillin-C, kaempferide, drupanin and p-coumaric acid as main compounds by LC-MS analysis. The nanoemulsion developed by phase inversion method was characterized and stable under thermal-stress and centrifugation conditions. Biological properties evaluated were effectively maintained by the formulation. It was concluded that the nanoemulsion can be used as a food preservative, preventing degradation and masking the propolis off-flavor.


Assuntos
Anti-Infecciosos , Antioxidantes , Conservantes de Alimentos , Própole , Anti-Infecciosos/análise , Anti-Infecciosos/química , Antioxidantes/análise , Antioxidantes/química , Emulsões/análise , Emulsões/química , Conservantes de Alimentos/análise , Conservantes de Alimentos/química , Nanotecnologia , Própole/análise , Própole/química
19.
Colloids Surf B Biointerfaces ; 177: 188-195, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-30738325

RESUMO

Nowadays diabetes, as a metabolic disorder, is increasing at an alarming rate. Glycation and production of advanced glycation end products (AGEs) is the most important factor involved in diabetic complications. Due to the side effects of synthetic drugs, the demand for natural anti-diabetic herbal medicines has increased. Propolis is a natural and resinous material, which iscollected by honeybees. Due to the impact of nanotechnology in medicine and the advantageous role of nanoparticles in treatment, nano-propolis particles (PNP) were prepared. The anti-glycation effect of PNP at various concentrations was investigated on human hemoglobin (Hb) glycation and fructation and compared with aspirin as a common anti-glycation agent using glycation specific AGE fluorescence, AGE-specific absorbance and circular dichroism (CD) methods. Fluorescence spectroscopy results showed that PNP inhibited the formation of AGEs in Hb glycation and fructation by glucose and fructose, respectively. CD results revealed that PNP caused an increase in Hb beta-sheet content while decreasing the alpha helical content. Additionally, the results of UV-Vis spectroscopy and fluorescence emission of heme degradation products revealed the protective effect of PNP on heme during glycation and fructation of human Hb. It is notable that the synergistic effects of combined propolis nanoparticles and aspirin is more than either of them alone. However, having said that, PNP as a natural product has a potential to be an effective drug in the treatment of diabetes.


Assuntos
Hemoglobinas/química , Hemoglobinas/metabolismo , Nanopartículas/química , Própole/química , Glicosilação , Humanos , Tamanho da Partícula , Conformação Proteica , Propriedades de Superfície
20.
Chem Biodivers ; 16(5): e1800442, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30725525

RESUMO

Propolis samples from north-west Argentina (Amaicha del Valle, Tucumán) were evaluated by palynology, FT-IR spectra, and RP-HPTLC. In addition, the volatile fraction was studied by HS-SPME-GC/MS. The botanical species most visited by Apis mellifera L. near the apiaries were collected and their RP-HPTLC extracts profiles were compared with propolis samples. In addition, GC/MS was performed for volatile compounds from Zuccagnia punctata Cav. (Fabaceae). FT-IR spectra and RP-HPTLC fingerprints of propolis samples showed similar profiles. In RP-HPTLC analyses, only Z. punctata presented a similar fingerprint to Amaicha propolis. The major volatile compounds present in both were trans-linalool oxide (furanoid), 6-camphenone, linalool, trans-pinocarveol, p-cymen-8-ol, and 2,3,6-trimethylbenzaldehyde. Potential variations for the Amaicha del Valle propolis volatile fraction as consequence of propolis sample preparation were demonstrated.


Assuntos
Compostos Fitoquímicos/química , Própole/química , Argentina , Cromatografia Líquida de Alta Pressão , Fabaceae/química , Fabaceae/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Microscopia , Óleos Voláteis/química , Compostos Fitoquímicos/isolamento & purificação , Folhas de Planta/química , Folhas de Planta/metabolismo , Análise de Componente Principal , Própole/isolamento & purificação , Microextração em Fase Sólida , Espectroscopia de Infravermelho com Transformada de Fourier , Compostos Orgânicos Voláteis/química , Compostos Orgânicos Voláteis/isolamento & purificação
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