A robust electrochemical sensor based on AgNWs@MoS2 for highly sensitive detection of thiabendazole residues in food samples.

Thiabendazole (TBZ), a highly toxic phosphorothioate insecticide commonly used in postharvest fruit management, has the potential to cause detrimental effects on human health as an endocrine disruptor. In this study, an electrochemical sensor was developed to detect TBZ by modifying MoS2 on silver nanowires (Ag NWs@MoS2) and integrating them onto a glassy carbon surface. Cyclic voltammetry revealed that TBZ underwent an irreversible, diffusion-controlled process on Ag NWs@MoS2, leading to a two-fold increase in peak current compared to unmodified MoS2. Square wave voltammetry facilitated TBZ detection, and the sensor exhibited a linear range of 0.05-10 µM with a high coefficient of determination (R2 = 0.9958) and a limit of detection (LOD) of 1.75 nM (signal-to-noise ratio = 3). The sensor's applicability for food safety monitoring was verified through TBZ analysis in pear and apple samples, achieving recoveries of 95.5-103.6% with RSDs in the range of 1.98-3.25%.

Dialdehyde starch-enclosed silver nanoparticles substrate with controlled-release "hotspots" for ultrasensitive SERS detection of thiabendazole.

Pesticide residues have long been a major concern for food safety. In this study, a dialdehyde starch-encapsulated silver nanoparticles composite with controlled-release "hotspots" was developed as a surface-enhanced Raman scattering (SERS) substrate. At room temperature, most of the Ag NPs were encapsulated in dialdehyde starch, which is beneficial for improving stability, and when heated to the gelatinization point, Ag NPs are completely released and abundant hot spots are formed. We demonstrated sensitive detection of thiabendazole (TBZ) in or on the surface of an apple by means of two ways, i.e., detecting the analyte in solution after pretreatment and in-situ detecting the analyte by using a flexible paper-based substrate. The results showed that the detection limits of TBZ by the two ways were 0.052 ppm and 0.051 ppm respectively, and the recoveries of TBZ range from 96.80 % to 105.46 %. Overall, this SERS substrate shows great potential for pesticide residue detection in food.

Efficient self-cleaning and antibacterial ceramics with ultra-low doping and high exposure of silver.

The secondary bacterial infection of COVID-19 is known to contribute significantly to mortality rates. Silver (Ag)-based antibacterial ceramics have emerged as a prominent solution for daily antibacterial applications, aiming to minimize the reliance on disinfectants while safeguarding human health. However, the fabrication of Ag-based antibacterial ceramics with low Ag content, high dispersion, and high exposure still faces challenges. In this work, an innovative method was proposed to doping Ag nanoparticles (Ag NPs) into glass ceramics (GC) via a "melt-freeze" method, then efficient and stable Ag-doped antibacterial ceramics (GC-xAg@BiOCl) were fabricated through facile in-situ HCl etching GC. Results indicate that the low Ag content (0.03 mol%) and high dispersion of Ag NPs are fully exposed and anchored on the surface, and constructed Schottky junction Ag/BiOCl contributed to antibacterial and photocatalytic activity. The degradation rates of norfloxacin and methylene blue by GC-0.25Ag@BiOCl can reach 71.0% and 55.3% under visible light irradiation, respectively. Moreover, The GC-0.25Ag@BiOCl exhibited significant antibacterial activity against Escherichia coli (E.coli) and Staphylococcus aureus (S. aureus), with E.coli at 7.3 log10 cfu/mL and S. aureus at 7.0 log10 cfu/mL completely inactivated under visible light irradiation. Additionally, the antibacterial mechanism and charge transfer mechanism were explored.

Modified magnesium oxide/silver nanoparticles reinforced poly (butylene succinate-co-terephthalate) composite biofilms for food packaging application.

MgO/Ag nanoparticles (NPs) were surface-modified with titanate coupling agent titaniumtriisostearoylisopropoxide (NDZ-130). A new antibacterial biofilm for food packaging was synthesized by combining the modified MgO/Ag NPs with poly (butylene succinate-co-terephthalate) (PBST). The modification improved the compatibility between the MgO/Ag NPs and the PBST matrix. The effects of the modified MgO/Ag NPs on biofilm mechanical, barrier, thermal, antibacterial and food preservation properties were evaluated. Compared with the PBST/MgO/Ag composite film, the modified PBST/MgO/Ag composite film showed an increase in tensile strength (TS) of 8.71% and elongation at break (EB) of 16.66%, additionally decreasing water vapor permeability (WVP) by 42.86%. The composite film also exhibited over 95% inhibition of Staphylococcus aureus and Escherichia coli. The modified PBST/MgO/Ag composite film avoided microbial contamination and preserved cherry tomatoes while maintaining moisture and firmness for six days. All results indicated that the prepared biofilms have a high potential for use as food packaging films.

Responsive luminescent silver-based metal-organic frameworks for highly sensitive and selective detection of hydrogen sulfide in biological system via a self-assembled headspace separation device.

As a highly toxic gas pollutant and also an endogenous gaseous signaling molecule existing in a variety of physiological processes, the rapid and accurate in-field detection of hydrogen sulfide is of great concern. Nevertheless, two drawbacks as for the optical probes for H2S detection, taking about a long time to reach the optical signal balance or the low selectivity, always exist. Herein, by using a highly photoluminescent and H2S-stimuli responsive silver-based metal-organic frameworks (MOFs): Ag-BDC (BDC = 1, 4-benzene dicarboxylate), we demonstrated that the luminescence intensity of Ag-BDC MOFs was inversely proportional to the concentration of H2S due to the Ag-S coordination and the obstruction of ligand-to-metal charge transfer (LMCT) transition process, and there was a quick response time of below 3.0 min. Combined with a simple customized device to separate H2S from the sample, the selectivity of the method for H2S detection could be greatly improved, and no interference would be caused even if the other sulfur-containing species coexisted. The luminescence probe presented a favorable sensitivity within a linear range of 0.1-1000 µM along with a detection limit of 23.7 nM. When employed to assay the endogenous sulfide level in the human serum and mouse brain tissue, the approach showed recoveries from 96.3% to 102% with relative standard derivation (RSD) less than 2.0%. By the integration of the responsive luminescent silver-based MOFs with a simple self-assembled headspace separation device, obviously the present strategy could be beneficial to the development and design of the in-field fast H2S measurement, possessing particular advantages in biological systems to eliminate the potential interferences.

The performance of hypochlorous acid modified Ag nanoparticle-based assay in the determination of total antioxidant capacity of Boswellia Serrata and Aronia.

In this study, the SNPAC method was developed by using hypochlorous (ClO-) at pH 9.3. Polyvinylpyrrolidone (PVP) was used as a stabilizer. The existence of HOCl enables to formation stronger and more stable yellow emission which gives sensitivity. As a result, lower LOD and LOQ values were achieved by the AgNP-HOCl method. The obtaining lower values was attributed to the reoxidation of silver atoms with ClO- ions in the presence of PVP. The newly modified method was applied to the total antioxidant capacity (TAC) measurement of Boswellia Serrata, Aronia Melanocarpa tea, and juice. The results were compared with the results obtained by application of CUPRAC (Cupric ion Reducing Antioxidant Capacity) and FRAP (Ferric Reducing Antioxidant Power) assays. The TAC values of Boswellia Serrata, Aronia Melanocarpa tea, and juice were found as 6.18, 137.98, and 12.08 mmol TEAC/kg, respectively.

A "signal-off" electrochemiluminescence immunosensor based on electron transfer between core-shell emitter Ag@SiO2 and quencher CeO2.

Silver nanoparticles (Ag·NPs) show promising advantages in electrochemiluminescence (ECL) owing to their favorable optical properties and biocompatibility. However, their susceptibility to oxidation and degradation in the presence of air adversely affects ECL intensity. In this study, we employed a sandwich sensing platform using silica-coated silver nanoparticles (Ag@SiO2) as a novel luminescent material and cerium dioxide (CeO2) as an ECL signal quencher for sensitive neuro-specific enolase (NSE) detection. The core-shell structure protected Ag NPs within the silica (SiO2) layer, enhancing their ECL luminescence properties by reducing external environmental influence and preventing Ag NPs aggregation. Amino-functionalized CeO2 efficiently diminished Ag@SiO2 ECL emission through electron transfer, resulting in a "signal-off" detection mode with high sensitivity and accuracy. The detection limit reached 1.66 fg/mL, and the detection range spanned from 100 fg/mL to 500 ng/mL, showcasing a powerful biomolecule detection strategy.

An innovative and universal dual-signal ratiometric spectro-electrochemical imprinted sensor design for sandwich type detection of anticancer-drug, gemcitabine, in serum samples; cross validation via computational modeling.

An innovative and universal imprinted sensor design for sandwich type detection of gemcitabine (GMT) in human serum samples is described. GMT is widely used in the treatment of different tumors, such as lung, ovarian, pancreatic, and breast cancer. The serum albumin-drug interaction was translated to design a multifunctional, ratiometric and dual mode silver nanoparticle based probe (BSA-Ag nanoprobe), as a read out system. Subsequently, polypyrrol imprinted drug receptor sites was engineered to selectively capture the GMT on the transducer surface. The GMT was sandwiched between imprinted receptor surface and BSA-Ag nanoprobe to generate the spectro-electrochemical signals. The formation of nanoprobe was confirmed through various characterization techniques, including X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, micro-Raman spectroscopy, Dynamic light scattering (DLS), and UV-Visible (UV-Vis) analysis, while each step of sensor fabrication was characterized via field emission scanning electron microscope (FE-SEM), Static water Contact angle measurements, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). Different variable parameters were optimized to improve the analytical performance of the sensor design. Under optimal conditions, spectro-electrochemical sensor permitted linear ranges between 1 and 200 µmol L-1 and 0.5-200 µmol L-1, with limits of detection (LOD) of 0.4 µmol L-1 and 0.15 µmol L-1 respectively. Furthermore, the designed sensor successfully differentiated the serum samples of lung cancer patients and healthy volunteers. The obtained results were validated with standard Liquid chromatography-mass spectrometry (LC/MS) analysis of the patients and healthy volunteer's serum samples. Lastly, density functional theory (DFT) and molecular docking calculations revealed the enhanced GMT binding capability of molecularly imprinted polypyrrole and molecular level interaction between the GMT and BSA, to validate the sandwich sensor design.

Handheld UV spectrophotometer device for detection of methamphetamine hydrochloride based on supramolecular sensing platform.

The realization of drug detection in drug-using crime sites can provide law enforcement officials with direct evidence. This research has developed and demonstrated an easy-to-use handheld sensor that can quickly detect methamphetamine (MA) in the field. The core of the handheld UV spectrophotometer device (HUVSD) is the STM32F103 series of single-chip micro-controller, which has a 32-bit microcontroller and two embedded 12-bit high-precision analog-to-digital converter (ADC) modules. Through Bluetooth-wireless transmission protocol, the spectral information can be displayed in the cell phone's app, and it is possible to visually determine whether the test sample contains methamphetamine hydrochloride substances based on the characteristic peak at 410 nm. The readily available and inexpensive inducible compound 3A and the phosphate pillar[5]arene@silver nanoparticle (PP5@AgNPs) colloidal solution were used as the reactants. The PP5@AgNPs colloidal solution and 3A were mixed and reacted at room temperature, and the color changed to gray-black. The color change was caused by the aggregation of AgNPs induced by the molecular recognition between the induction compound 3A and PP5 on the AgNPs surface. After continuing to add the drug MA, the color of the colloidal solution turned yellow again. This is due to the occurrence of competitive molecular recognition, and the interaction between PP5 and 3A/MA was investigated by molecular docking simulations. The HUVSD has high sensitivity, simple equipment, time-saving, color change visualization and suitable for on-site deployment. It only needs a Pasteur pipette, which has great potential to realize rapid on-site detection.

Surface-enhanced Raman spectroscopy for characterization of supernatant samples of biofilm forming bacterial strains.

Staphylococcus epidermidis is considered major cause of nosocomial infections. Its pathogenicity is mainly due to the ability to form biofilms on different surfaces, particularly indwelling medical devices. This bacterium consists of different strains consisting of non, medium and strong biofilm forming ones. Surface-enhanced Raman spectroscopy (SERS) is a powerful analytical technique that can be used to detect and analyze biochemical composition of the supernatant samples of different strains of bacteria including non, medium and strong biofilm forming bacterial strains. SERS is a powerful technique for the robust, reliable, rapid detection and discrimination of bacteria in the form of characteristic SERS spectral features which can be used for detection and classification. SERS is used to differentiate three classes of bacteria with respect to their biofilm forming ability. Silver nanoparticles (Ag NPs) are used as SERS substrate and synthesized with chemical reduction method. Principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) are used to discriminate SERS spectral data sets of non, medium and strong biofilm forming bacteria. PLS-DA analysis is a multivariate statistical technique that can be used to analyze data from bacterial sets.

Discrimination of resected glioma tissues using surface enhanced Raman spectroscopy and Au@ZrO2 plasmonic nanosensor.

Gliomas present one of the most prevalent malignant tumors related to the central nervous system. Surgical extraction is still a preferred route for glioma treatment. Nonetheless, neurosurgeons still have a considerable challenge to detect actual margins of the targeted glioma intraoperatively and correctly because of its great natural infiltration. Here we evaluated the possibility of using surface-enhanced Raman spectroscopy to analyze freshly resected brain tissues. The developed method is based on the application of Au@ZrO2 nanosensor. The plasmonic properties of the sensor were first tested on the analysis of Rhodamine 6G, where concentrations down to 10-7 mol/L can be successfully detected. We also compared the performance of the nanosensor with silver plasmonic nanoparticles, where similar results were obtained regarding the reduction of the fluorescence background and enhancement of the intensity of the measured analytical signal. However, application of silver nanospheres led to increased variations in spectral data due to its probable aggregation. Applied ZrO2@Au nanosensor thus dramatically lowers the fluorescence present in the Raman data, and considerably improves the quality of the measured signal. The developed method allows for rapid discrimination between the glioma's periphery and central parts, which could serve as a steppingstone toward highly precise neurosurgery.

An efficient multi-functional ternary reusable nanocomposite based on chitosan@TiO2@Ag NP immobilized on cellulosic fiber as a support substrate for wastewater treatment.

To effectively remove heavy metals, organic contaminants, and pathogenic bacteria from wastewater, an efficient multi-functional ternary nanocomposite based on chitosan (CS), titanium dioxide (TiO2 NP), and silver nanoparticles (Ag NP) was prepared. Different tools were used to confirm the successful synthesis of the CS/TiO2 NP/Ag NP nanocomposite. Then, the CS/TiO2 NP/Ag NPnanocomposite was immobilized on the cellulosic fiber as a support substrate for its easy removal and reuse. On a lab scale, CS/TiO2 NP/Ag NP nanocomposite@cellulosic fiber was used to remove Cu (II) ions, methyl orange (MO), and methylene blue (MB), as well as inhibit microbes. The results demonstrate that the greatest removal of Cu (II) ions was 95 % at a concentration of 50 mg/L, pH 5, a temperature of 25 °C, an agitation speed of 200 rpm with 1 g adsorbent dose, and a contact time of 150 min. The pseudo-second-order model explained the batch adsorption kinetics well, while the Langmuir model explained the adsorption isotherm well with an adsorption capacity of 7.71 mg/g. Adsorption thermodynamic parameters revealed that adsorption is a spontaneous, exothermic, increased randomness, and non-specific chemisorption approach. The photodegradation of MO and MB by CS/TiO2 NP/Ag NP nanocomposite@cellulosic fiber was investigated. The results reveal that at pH 3, the MO dye showed the highest photodegradation percentage (90 %), while the MB dye displayed the highest photodegradation percentage (94 %) at pH 11, after an irradiation time of 120 min under visible light. The rate constants for MO and MB were 0.01218 and 0.01412 min-1, respectively. The results antimicrobial activities showed that the CS/TiO2 NP/Ag NP nanocomposite@cellulosic fiber showed excellent antibacterial activity against S. aureus (95 ± 2 %) and E. coli (93 ± 3 %) as well as good antifungal activity against C. albicans (77 ± 2 %).

Cheap and portable paper chip with terrific oxidase-like activity and SERS enhancement performance for SERS-colorimetric bimodal detection of intracellular glutathione.

Glutathione (GSH) acts a crucial role in the normal operation of manifold life activities and is closely bound up with many human diseases. Here, a SERS-colorimetric bimodal paper-based biosensor based on Mn-doped CDs/silver nanoparticles (Mn-CDs/AgNPs) has been fabricated for high-efficiency quantification of intracellular GSH. The Mn-CDs/AgNPs with fine oxidase-like characteristic and SERS enhancement ability has been assembled onto the Whatman filter paper (WFP) to cleverly fabricate paper chip (Mn-CDs/AgNPs@WFP) which can trigger the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) into blue TMBox and simultaneously enhance the SERS signal of TMBox. However, the introduction of GSH inhibits the oxidation of TMB, leading to color fading of paper chip and diminishment of SERS signal. Considering this, the bimodal paper-based sensing platform can be exploited for SERS-colorimetric detection of GSH, manifesting excellent selectivity, reliable stability, and satisfactory precision. The detection limits of SERS and colorimetric detection modes are as low as 0.41 µM and 0.53 µM, respectively. Furthermore, this proposed bimodal biosensor has been successfully utilized for the determination of intracellular GSH and validated by commercial GSH assay kit, which provides a mighty and convenient tool for intracellular GSH detection and can boost future effort about exploitation of other multimode paper-based biosensors as well as promote their appliances in disease diagnosis.

Comprehensive response of microbes to Ag and Ag2S nanoparticles and silver spatial distribution in constructed wetlands.

This study investigated functional bacteria, key enzymes, and nitrogen metabolism in vertical flow constructed wetlands (CWs) after exposing to silver, silver sulfide nanoparticles (Ag NPs and Ag2S NPs), and silver iron (Ag+), and silver spatial distribution in CWs for 155 days. Ag NPs and Ag2S NPs affected species richness and diversity whereas Ag+ showed the higher the species diversity indices. Sequencing analysis exhibited that Ag NPs or Ag+ significantly inhibited nitrogen metabolic process by hindering the relative activity of functional enzymes, downregulating relative abundances of nrfA, norB and napA for Ag NPs, nxrA gene for Ag+, while Ag2S NPs inhibited relative abundance of nirA. The above results confirmed that NPs or Ag+ significantly reduced nitrogen removal and Ag NPs mainly inhibited NO3--N removal while Ag+ significantly suppressed NH4+-N removal. This study also found that CWs could effectively remove NPs or Ag+ (about 98 %), and nanoparticles showed higher translocation factors (TFs) values (0.81-1.15 or 0.36), indicating nanoparticles transported easily through substrate layers.

Rapid detection of nanoplastics down to 20 nm in water by surface-enhanced raman spectroscopy.

Plastic pollution represents a pressing global environmental issue, with microplastics (MPs) and nanoplastics (NPs) being ubiquitously found in both food and the environment. However, the investigation of NPs has been hampered by limited detection technologies, necessitating the development of advanced techniques. This study introduces a sol-based surface-enhanced Raman spectroscopy (SERS) approach for the swift detection of MPs and NPs in aqueous environment. By leveraging the aggregation effect between silver nanoparticles (Ag nanoparticles) and plastic particles, the plastic Raman signals is significantly enhanced, effectively lowering the detection limit. Utilizing Ag nanoparticles, plastic particles as small as 20 nm were detected in liquid samples, with a detection limit of 0.0005%. With the developed method, nanoplastic particles in seafood packaging samples were successfully tested, with concentration found to be at µg/L level. This method offers a rapid, economical, and convenient means of detecting and identifying MPs and NPs. The sensitivity of the method allows for capturing plastic signals within 2 min, making it valuable for aquatic environment contamination detection. SERS technology also holds promise for rapid plastic solution detection, potentially becoming a fast detection method for food safety.

Cytotoxic, antioxidant, and antiglycation activities, and tyrosinase inhibition using silver nanoparticles synthesized by leaf extract of Solanum aculeatissimum Jacq.

The present study aimed to determine the biological properties of an extract of Solanum aculeatissimum aqueous extract (SaCE) alone as well as silver nanoparticles (AgNPs) generated by green synthesis utilizing S. aculeatissimum aqueous extract (SaCE). These synthesized SaCE AgNPs were characterized using UV-VIS spectrophotometry, scanning transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), zeta potential (ZP), dynamic light scattering (DLS). Determination of total polyphenols, flavonoids, saponins content was conducted. In addition, high performance liquid chromatography-mass spectrometry (HPLC-MS) was employed to identify constituents in this extract. Antioxidant activity was determined by DPPH radical scavenging and ferric ion reducing power (FRAP) methods. Antiglycation activity was demonstrated through relative mobility in electrophoresis (RME) and determination of free amino groups. The inhibitory activity on tyrosinase was also examined. Molecular docking analyses were performed to assess the molecular interactions with DNA and tyrosinase. The antitumor activity SaCE was also measured. Phytochemical analysis of SaCE and AgNPs showed presence polyphenols (1000.41 and 293.37 mg gallic acid equivalent/g), flavonoids (954.87 and 479.87 mg rutin equivalent/g), saponins (37.89 and 23.01% total saponins), in particular steroidal saponins (aculeatiside A and B). Both SaCE and AgNPs exhibited significant antioxidant (respectively, 73.97%, 56.27% in DPPH test, 874.67 and 837.67 µM Trolox Equivalent/g in FRAP test) and antiglycation activities (72.81 and 67.98% free amino groups, results observed in RME). SaCE and AgNPs presented 33.2, 36.1% inhibitory activity on tyrosinase, respectively. In silico assay demonstrated interaction between steroidal saponins, DNA or tyrosinase. SaCE exhibited antitumor action against various human tumor cells. Data demonstrated that extracts SaCE alone and AgNPs synthesized from SaCE presented biological properties of interest for application in new therapeutic formulations in medicine.

Wound healing potential of silver nanoparticles embedded in optimized bio-inspired hybridized chitosan soft and dry hydrogel.

Interactive wound dressings combining healing and antimicrobial potentials, besides ensuring patient compliance with a recognized wound care service gained considerable interest recently. Both hydrogel spray dried microparticles (HMP) and soft hydrogel (G) were prepared. The bio-inspired combinatory platform included natural bio-macromolecules namely: chitosan (CS) and collagen (COL) with wound healing enhancement and connective tissue building capabilities cross linked with the natural genipin (GN) to build a three dimensional structured matrix. The optimized plain hydrogel obtained by a box behnken design (BBD) program (G) scored maximum swelling and porosity. The network was hosted with green synthesized cefotaxime sodium (cef.Na) AgNPs reduced by the anabolic folic acid (FA). Both hydrogels exhibited good antimicrobial activity against gram +ve and -ve bacteria. The wound healing activity, evaluated in injured rats, showed >98 % and complete wound closure after two and three weeks respectively. Oxidative stress minimization was proved by the estimation of biochemical markers malondialdehyde (MDA) and superoxide dismutase (SOD) levels at the wound site.

Environmental behavior of silver nanomaterials in aquatic environments: An updated review.

The increasing applications of silver nanomaterials (nano-Ag) and their inevitable release posed great potential risks to aquatic organisms and ecosystems. Considerable attention has been attracted on their behaviors and transformations, which were critically important for their subsequent biological toxicities and ecological effects. Therefore, the summary of the recent efforts on the environmental behavior of nano-Ag would be beneficial for understanding the environmental fate and accurate risk assessment. This review summarized the studies on various physical, chemical and biological transformations of nano-Ag, meanwhile, the influencing factors (including the intrinsic properties and environmental conditions) and related mechanisms were highlighted. Surface structure and facets of nano-Ag, abiotic conditions and natural freeze-thaw cycle processes could affect the transformations of nano-Ag under different environmental scenarios (including freshwater, seawater and wastewater). The interactions with co-present components, such as chemicals and other particles, impacted the multiple processes of nano-Ag. Besides, the contradictory effects and mechanisms by several environmental factors were summarized. Lastly, the key knowledge gaps and some aspects that deserve further investigation were also addressed. Therefore, the current review aimed to provide an overall analysis of transformation processes of nano-Ag, which will provide more available information and pave the way for the future research areas.

Efficacy and moderators of metacognitive training for depression in older adults (MCT-Silver): A randomized controlled trial.

BACKGROUND: Up to 79 % of older adults with depression do not receive treatments commensurate with guideline recommendations. Metacognitive Training-Silver (MCT-Silver) is a low-intensity group training, which aims to reduce depressive symptoms by targeting (meta)cognitive beliefs. METHODS: A randomized controlled trial comparing MCT-Silver (n = 41) to cognitive remediation (n = 39) was conducted with older adults with major depressive disorder and/or dysthymia. Clinician-rated depression (Hamilton Depression Rating Scale [HDRS, primary outcome]), self-reported depression (Beck Depression Inventory-II [BDI-II]), negative cognitive beliefs, positive metacognitive beliefs, rumination, health status, quality of life, and neurocognitive functioning were assessed at baseline, eight weeks (post) and three months (follow-up). RESULTS: Both groups demonstrated moderate to large reductions in depression. No superior effects of MCT-Silver on clinician-rated depression (HDRS) were detected. MCT-Silver led to greater reductions in self-reported depression and rumination at post- and follow-up. Despite this, scores at post- and follow-up assessments were similar for both groups. MCT-Silver's effect on depressive symptoms was moderated by baseline rumination, positive metacognitive beliefs and previous treatment experience. MCT-Silver was evaluated as superior according to patient appraisals. LIMITATIONS: Conclusions are limited by divergent findings on measures of depression and that the study was conducted during the COVID-19 pandemic. CONCLUSIONS: Whereas no superior effect of MCT-Silver was found for the primary outcome, there was a significant effect of MCT-Silver for self-reported depression and rumination. Patients endorsing rumination and positive metacognitive beliefs as well as those without previous psychological treatment may benefit more from MCT-Silver.

Hydrogel SERS chip with strong localized surface plasmon resonance for sensitive and rapid detection of T-2 toxin.

T-2 toxin is one of the naturally dangerous food contaminants, which is harmful to people and animals. Because of its strong toxicity and wide distribution, it is vital to develop a rapid and effective method for the detection of T-2 toxin. Herein, an excellent hydrogel surface-enhanced Raman scattering (SERS) chip is constructed for developing a novel SERS sensor to detect T-2 toxin using a portable Raman spectrometer. The SERS chip is prepared by in-situ Ca2+-mediated assembly of silver nanoparticles (AgNPs) in PVA solution, followed by a physical crosslinking possess. The assembled AgNPs produces a strong localized surface plasmon resonance (LSPR) at around 532 nm, which enables the high activity of SERS chip under the irradiation of 532 nm laser. Additionally, the unique structure of hydrogel makes the obtained chip show excellent reliability and anti-interference ability in detection. As a result, the developed SERS sensor shows many obvious advantageous including free of complex sample pretreatment (only a simple extraction), fast response (5 min), low limit of detection (0.41 ppb), wide detection range (1-10000 ppb), good recoveries (90.26-101.81 %) and relative standard deviations (2.8-6.7 %). Therefore, this SERS sensor provides a promising choice for rapid scanning and sensitive detection of trace T-2 toxin in complex matrices.