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1.
Food Chem ; 332: 127385, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-32623125

RESUMO

A promising way of processing Baltic herring, Clupea harengus membras, is turning the fish into boneless mince. However, Baltic herring is prone to lipid oxidation, which possesses a challenge for industrial applications. The aim of this work was to study the efficacy of press cakes from Finnish berries and a supercritical CO2 plant extract to limit lipid oxidation during frozen storage of Baltic herring mince and to determine the impact of these additions on consumer acceptance in a fish product. Peroxide value, formation of volatile oxidation products and loss of polyunsaturated fatty acids showed that the tested natural additives decreased oxidation to a greater or similar extent as conventional antioxidants during 10-month storage. While potential of berry press cakes and plant extracts as "green label antioxidants" was shown, consumer study indicated need for further research to reach both optimal antioxidative efficacy and sensory properties.


Assuntos
Cromatografia com Fluido Supercrítico/métodos , Produtos Pesqueiros/análise , Extratos Vegetais/química , Animais , Antioxidantes/química , Dióxido de Carbono/química , Ácidos Docosa-Hexaenoicos/análise , Ácido Eicosapentaenoico/análise , Peixes , Congelamento , Lipídeos/análise , Fenóis/análise , Compostos Orgânicos Voláteis/análise
2.
Food Chem ; 330: 127205, 2020 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-32521397

RESUMO

The incidence of endocrine disruptors, both possible (glyphosate and glufosinate), and demonstrated (perchlorate and chlorate), was estimated in baby food commodities (meat, fish, cheese, vegetable and fruit). Ion-chromatography coupled to high resolution mass spectrometry analysis of the 105 samples did not show traces of glyphosate, glufosinate or their metabolites, while in 10.5% of the samples a quantifiable amount of perchlorate was found. Some samples based on fruit and vegetables revealed a substantial amount of chlorate, especially the preparations that contained carrots and potatoes: five samples were in a concentration range of 40-120 µg kg-1, while one homogenized pear sample reached 372.2 µg kg-1. The pure meat samples revealed occasional chlorate appearance, with less than 10 µg kg-1. This is the first report of chlorate evaluated in various types of baby food and may serve as symptomatic data regarding its occurrence in infant/toddler diets. Therefore, effective monitoring programs and subsequent strict regulations are strongly required.


Assuntos
Cloratos/análise , Contaminação de Alimentos/análise , Herbicidas/análise , Alimentos Infantis/análise , Percloratos/análise , Animais , Cloratos/química , Produtos Pesqueiros/análise , Frutas/química , Herbicidas/química , Carne/análise , Percloratos/química , Verduras/química
3.
Food Chem ; 327: 127002, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-32438262

RESUMO

This study was designed to investigate proteome changes in Japanese puffer fish (Takifugu rubripes) during short- and long-term frozen storage. In total, 1484 proteins were quantified, and 164 proteins were identified as differential abundance proteins (DAPs) in Japanese puffer fish from two frozen storage treatment groups (14 days and 60 days) compared with the fresh control group. Correlation analysis between the DAPs and quality traits of the puffer fish muscle showed that 106 proteins were correlated closely with colour and texture (hardness, elasticity, and chewiness). Bioinformatics analysis revealed and Western blot analysis verified that Putative prothymosin alpha species, Bridging integrator 3, NADH: the ubiquinone oxidoreductase subunit and Mx species are candidate biomarkers for puffer fish properties. This study offers valuable evidence to improve the quality control and monitoring of Japanese puffer fish during transportation and storage.


Assuntos
Biomarcadores/análise , Produtos Pesqueiros/análise , Proteínas de Peixes/análise , Takifugu , Animais , Cor , Biologia Computacional/métodos , Análise de Alimentos/métodos , Qualidade dos Alimentos , Armazenamento de Alimentos , Congelamento , Músculo Esquelético/química , Precursores de Proteínas/análise , Timosina/análogos & derivados , Timosina/análise
4.
Food Chem ; 327: 127078, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-32454272

RESUMO

The potential of cross-processing herring or salmon by-products with brown seaweed, shrimp peeling by-products and lingonberry press cake (called "helpers") to minimize lipid oxidation during acid/alkaline pH-shift protein isolation was evaluated. Lingonberry press cake remarkably reduced levels of lipid hydroperoxides, malondialdehyde (MDA) and 4-hydroxy-trans2-hexenal (HHE) in both herring and salmon protein isolates (PI) while seaweed and shrimp by-product mitigated generation of MDA and HHE in herring PI. The salmon PI, however, by itself had very low oxidation levels. Cross-processing reduced protein yield compared with the classical pH-shift process by diminishing protein solubility, but still, the alkaline process version was promising. The color of the PIs varied greatly between different process combinations, reflecting partitioning of natural pigments into isolates. Altogether, cross-processing seems a promising tool for inhibition of lipid oxidation during pH-shift processing of sensitive fish by-products, with the alkaline version and lingonberry press cake being closest at hand for further studies.


Assuntos
Produtos Pesqueiros/análise , Peixes , Lipídeos/química , Alimentos Marinhos , Alga Marinha/química , Vaccinium vitis-Idaea/química , Animais , Concentração de Íons de Hidrogênio , Oxirredução , Solubilidade
5.
Food Chem ; 326: 126942, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32407999

RESUMO

A non-targeted screening method based on ultrasound-assisted extraction followed by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS) was developed to screen for the presence of plastic-related chemicals (PRCs) in different types of food (fish, chicken, canned tuna, leafy vegetables, bread and butter). Eleven bisphenols were used as targeted compounds. Instrument linearity (r2 ≥0.98), inter-day precision (RSD ≤9.0%) as well as method detection limits (MDLs below 3.6 ng g-1) were satisfactory. Recoveries of the eleven bisphenols ranged from 76% to 122% among the different food matrices. The method was applied to food collected from Montreal, Canada in 2017-2018. The non-targeted screening approach identified a range of contaminants in different food matrices, including BPA, BPS, bis(2-ethylhexyl) adipate, dibutyl adipate, hexadecyl methacrylate and Irganox®1076. Further research is suggested to investigate the concentration of these PRCs, the consumption habits of average and specific populations and the potential routes of contamination.


Assuntos
Contaminação de Alimentos/análise , Plásticos/análise , Animais , Compostos Benzidrílicos/análise , Pão/análise , Canadá , Galinhas , Cromatografia Líquida de Alta Pressão , Produtos Pesqueiros/análise , Peixes , Limite de Detecção , Carne/análise , Fenóis/análise , Espectrometria de Massas em Tandem/métodos , Verduras/química
6.
Food Chem ; 321: 126744, 2020 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-32278986

RESUMO

This study aimed to evaluate the shelf-life of mechanically filleted well-fed Atlantic mackerel during frozen storage at -25 °C and effect of treatment with antioxidants (sodium erythorbate and a polyphosphate mixture) and different antioxidant application methods (dipping, spraying and glazing). Both physicochemical measurements and sensory analysis were applied. Antioxidant treatments prolonged shelf-life of mackerel. Sensory analysis indicated that untreated fillets had a shelf-life of less than 2.5 months, while all antioxidant treated fillets exceeded that. The most effective treatment, dipping fillets into a sodium erythorbate solution, yielding a shelf-life of 15 months. Physicochemical methods used to evaluate degradation of lipids in the fillets were free fatty acids (FFA), lipid hydroperoxides (PV) and thiobarbituric acid reactive substances (TBARS). They did not correlate with sensory results and might therefore be a questionable choice for evaluation of oxidation and development of rancid flavour and odour in complex matrixes such as Atlantic mackerel.


Assuntos
Antioxidantes/química , Produtos Pesqueiros , Armazenamento de Alimentos/métodos , Perciformes , Paladar , Animais , Ácido Ascórbico/química , Ácidos Graxos/análise , Produtos Pesqueiros/análise , Congelamento , Humanos , Lipídeos/química , Oxirredução , Polifosfatos/química , Substâncias Reativas com Ácido Tiobarbitúrico/química , Fatores de Tempo
7.
Food Chem ; 319: 126592, 2020 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-32193062

RESUMO

A fast, fit-for-purpose, high-throughput method was developed and validated for the analysis of 302 targeted contaminants in catfish muscle. Targeted contaminants included pesticides and metabolites with US regulatory levels, plus other lipophilic pesticides and environmental contaminants: PAHs, PCBs, PBDEs and other flame retardants. The sample preparation was based on QuEChERS extraction. The extract was split and analyzed by UHPLC-MS/MS for 128 analytes after filtering and by low pressure (LP) GC-MS/MS for 219 analytes after an automated robotic micro-SPE cleanup. The method was validated at four spiking levels (5, 10, 20 and 40 ng/g) with 10 replicates per level, and satisfactory recoveries (70-120%) and RSDs (≤20%) were achieved for 80% of the analytes. The method was further validated with NIST SRMs 1946 and 1947 and applied for the analysis of 22 market fish samples. Six pesticides and one PAH were found in the market fish samples, all below US regulatory levels.


Assuntos
Peixes-Gato , Poluentes Ambientais/análise , Produtos Pesqueiros/análise , Contaminação de Alimentos/análise , Ensaios de Triagem em Larga Escala/métodos , Resíduos de Praguicidas/análise , Animais , Automação Laboratorial , Cromatografia Líquida de Alta Pressão , Retardadores de Chama/análise , Análise de Alimentos/métodos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Éteres Difenil Halogenados/análise , Bifenilos Policlorados/análise , Hidrocarbonetos Policíclicos Aromáticos/análise , Reprodutibilidade dos Testes , Espectrometria de Massas em Tandem/métodos
8.
J Food Sci ; 85(4): 1037-1044, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32175601

RESUMO

Effects of lysozyme (LYS) combined with cinnamaldehyde (CA) on quality enhancement of olive flounder (Paralichthys olivaceus) fillets during refrigerated storage at 4 °C for 20 days were assessed. Changes of total viable count (TVC), K-value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), texture profile analysis (TPA), and trichloroacetic acid-soluble peptide (TCA-soluble peptide) in samples were determined periodically. Results demonstrated that the combination of LYS and CA treatment enhanced the antibacterial activity against S. putrefaciens and P. fluorescens, and lowered TVC values. Meanwhile, LYS combined with CA significantly retarded the increases of TBA value, TVB-N, K-value, and TCA-soluble peptide content compared to the control. Furthermore, the combined treatment also effectively maintained the texture properties of flounder fillets during the storage period. The efficiency was better than that of LYS or CA treatment alone. Thus, LYS combined with CA is promising in olive flounder shelf life extension.


Assuntos
Acroleína/análogos & derivados , Produtos Pesqueiros/análise , Conservação de Alimentos/métodos , Muramidase/química , Acroleína/análise , Animais , Linguados , Conservação de Alimentos/instrumentação , Conservantes de Alimentos/análise , Armazenamento de Alimentos , Nitrogênio/análise
9.
PLoS One ; 15(2): e0228454, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32053624

RESUMO

In commercial wild capture pelagic fisheries it is common practice to crowd catches to high densities to allow efficient pumping onboard. Crowding during the final stages of purse seine capture for small pelagic species often results in intense and sustained behavioural escape responses. Such a response may trigger a shift in energy production from aerobic to anaerobic pathways and result in metabolic acid accumulation and exhaustion of intracellular reserves of ATP. Where there is insufficient time or opportunity to recover to physiological equilibrium before death, pre-mortem stress may be an important determinant of fillet quality, as has been shown for a variety of farmed fish species. However, there is currently a lack of knowledge related to the flesh quality implications of capture stress for wild captured species in European waters. Here we show that crowding results in a physiological stress response that has consequences for flesh quality in the wild captured species Atlantic mackerel (Scomber scombrus). Using small schools in tanks and aquaculture net pens in three separate experiments, we found crowding results in physiological changes in mackerel consistent with an acute stress response and anaerobic metabolism. Consequently, we found crowded fish had more acidic pre- and post-mortem muscle pH as well as indications of faster onset and strength of rigor mortis and increased cathepsin B & L activity. We examined fillet flesh quality after two and seven days of ice storage and found reduced green colouration, increased gaping (separation of muscle myotomes) and reduced textural firmness associated with fish which had been crowded. However, the effects on quality were dependant on experiment and/or storage time. These results indicate the potential of crowding capture stress to influence the flesh quality of an economically important species and may have important implications for the wild capture pelagic fishing industry.


Assuntos
Aglomeração , Pesqueiros , Músculos/fisiologia , Perciformes/fisiologia , Estresse Fisiológico , Animais , Autopsia , Produtos Pesqueiros/análise , Qualidade dos Alimentos , Concentração de Íons de Hidrogênio , Músculos/química , Músculos/patologia , Alimentos Marinhos/análise
10.
Food Chem ; 316: 126337, 2020 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-32036181

RESUMO

To allow value adding into foods, stabilizing strategies for fish by-products are needed based on their high susceptibility to hemoglobin (Hb)-mediated lipid oxidation. Here, three strategies for preventing lipid oxidation in herring (Clupea harengus) by-products during ice-storage were studied: (i) rinsing away Hb with water or 0.9% NaCl with/without antioxidants (Duralox-MANC, erythorbate and ethylenediamine-tetraacetic acid (EDTA)), (ii) incubation in water/0.9% NaCl with/without antioxidants, (iii) mincing with subsequent addition of the mentioned antioxidants. Only 10-18% Hb was rinsed away in (i), and the effect of this rinsing on peroxide value (PV) or TBA-reactive substances (TBARS) development was limited. Rinsing or incubating by-products in antioxidant solutions however significantly (p ≤ 0.05) increased shelf life from <1 day to >12 days; Duralox-MANC was particularly efficient. The presented strategies could hereby facilitate more diversified end-use of herring by-products from being 100% feed, to include also high-quality minces, protein isolates or oils for the food industry.


Assuntos
Antioxidantes/química , Peixes , Lipídeos/química , Animais , Produtos Pesqueiros/análise , Hemoglobinas/química , Oxirredução , Soluções
11.
J Chromatogr A ; 1619: 460973, 2020 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-32081487

RESUMO

Low levels of inorganic arsenic [As(III) and As(V)] in fishery products have been selectively isolated from fish extracts (1.0 g of wet fish samples pre-treated with 10 mL of 1:1 methanol/water under sonication at 25 °C for 30 min) by ionic imprinted polymer (IIPs) based solid phase extraction procedure (on-column mode). The selective adsorbent was synthesized using sodium (meta) arsenite as a template, 1-vinyl imidazole as a functional monomer, divinylbenzene as a cross-linker, and 2,2'-azobisisobutyronitrile as an initiator. Optimized pre-concentration conditions imply fish extract (10 mL) pH adjustment at 8.5 before loading (flow rate of 0.25 mL min-1), and elution with ultrapure water (2 mL) at 0.50 mL min-1. A pre-concentration factor of 50 was finally obtained after evaporation to dryness (N2 stream) and re-dissolution in 0.2 mL of ultrapure water before HPLC-ICP-MS. Synthesized material was found to pre-concentrate inorganic arsenic species; whereas organic arsenic compounds, mainly arsenobetaine (the major organoarsenic compound in fish/seafood products), were not found to interact with the adsorbent. The developed selective method gave limits of quantification of 1.05 and 1.31 µg kg-1 for As (III) and As (V), respectively, and good precision [relative standard deviations lower than 12% in fish extracts spiked at several As (III) and As (V) levels]. The proposed method was finally applied to the selective determination of As (III) and As (V) species in several fishery products.


Assuntos
Arsênico/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Produtos Pesqueiros/análise , Pesqueiros , Espectrometria de Massas , Polímeros/química , Extração em Fase Sólida/métodos , Animais , Arsenicais/química , Peixes , Íons , Limite de Detecção
12.
J Chromatogr A ; 1619: 460906, 2020 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-32007217

RESUMO

The aim of our work was to develop an analytical strategy to quantify naphthalene, acenaphthylene, acenaphthene, fluorene, phenanthrene and anthracene in fish products by on-line dynamic headspace extraction, followed by thermodesorption injection and gas chromatography analysis coupled with tandem mass spectrometry using electron ionization mode (DHS-TD-GC-EI-MS/MS). The developed protocol used 1 g of freeze-dried or oil sample supplemented with perdeuterated light PAHs. The sample was heated at [90 -100 °C], the headspace of the sample was swept by nitrogen and the trapping of the PAHs was carried out on a Tenax-type adsorbent placed at 25 °C. Analytes were thermodesorbed at 300 °C from the dried adsorbant and then cryofocused on a cooled injection system (CIS) at -25 °C before injection (12 °C s-1 up to 300 °C). The chromatographic separation of PAHs was carried out on a 5-MS type column (30 m × 0.25 mm, 0.25 µm) and the acquisition of the signals was performed in SRM following the transitions, involving the loss of one or two hydrogen atoms from the molecular ion. In view of the principle of extraction, the calibration curve was performed on a representative matrix or using the standard addition method. Quantification limits were determined between 0.01 and 0.6 ng g-1 of matrix from the method blank results. The method was validated by a series of multi-level supplemented matrix assays and by the analysis of a reference material from an inter-laboratory test (mussels, IAEA-432). The average of the expanded measurement uncertainty was from 9 to 44% for the four lightest PAHs, except for fluorene when the sample incubation was set at 90 °C. Occurrence measurements were performed on almost two hundred samples of molluscs, echinoderms and fish. The results have shown a quantification frequency greater than 66% for naphthalene and fluorene, at concentrations below a few ng g-1 of dry matter of fishery products. With this methodology, the light PAHs occurrence can now be measured in a wider range of foodstuffs in order to better characterize their contamination trends and the associated risk simultaneously.


Assuntos
Produtos Pesqueiros/análise , Cromatografia Gasosa-Espectrometria de Massas/métodos , Hidrocarbonetos Policíclicos Aromáticos/análise , Espectrometria de Massas em Tandem/métodos , Animais , Técnicas de Diluição do Indicador , Isótopos , Hidrocarbonetos Policíclicos Aromáticos/isolamento & purificação , Temperatura
13.
Food Chem ; 316: 126343, 2020 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-32045816

RESUMO

Surimi processing industry generates huge quantities of underutilized surimi processing by-products (SPB). This study aimed to investigate the potential antioxidant-cryoprotective and gelation-enhancing effects of SPB hydrolysates on silver carp (Hypophthalmichthys molitrix) surimi based on protein oxidation, protein degradation, and gel-forming ability analysis. Results showed that untreated surimi was particularly susceptible to frozen-induced protein degradation and oxidation as well as rapid deterioration in gelation properties. Compared with 4% sucrose-added surimi, partial replacement of sucrose with 2% trypsin- and alcalase-treated SPB hydrolysates effectively delayed the oxidation of cysteine, carbonylation of amino acids, loss of Ca2+-ATPase activity, and the destroy of structural integrity of myofibrillar protein in surimi. Moreover, addition of SPB hydrolysates significantly (P < 0.05) increased the initial gelation properties and reduced the loss in gelation and water-holding capacity in surimi with prolonged frozen storage. Therefore, SPB hydrolysates could be used as natural antioxidant-cryoprotectant and gel texture enhancer in freshwater fish surimi.


Assuntos
Carpas , Produtos Pesqueiros/análise , Proteínas de Peixes da Dieta/química , Hidrolisados de Proteína/química , Animais , Antioxidantes/farmacologia , Crioprotetores/farmacologia , Congelamento , Géis/química , Peso Molecular , Oxirredução
14.
Food Chem ; 313: 126135, 2020 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-31951883

RESUMO

Persistent halogenated compounds (PHCs) contamination has become a major concern over the world. Here we investigated occurrence, spatial distributions, congener profiles, as well as health risks of PHCs in farmed golden pompano in China using gas chromatography/mass spectrometry (GC/MS). The concentrations of PCBs, PBDEs and OCPs were in the range of 0.78-4.79 ng/g wet weight (ww), not detected (nd)-1.14 ng/g ww and 1.1-38.8 ng/g ww, respectively. Furthermore, ρ,ρ'-DDT, ο,ρ'-DDT and PCB 101 were the dominant PHC contaminants. The estimated daily intakes of PHCs through consumption of golden pompano were up to 12.86 and 131.34 ng/kg body weight/day based on the mean and 95th concentrations determined in golden pompano, respectively. Risk-based analysis indicates that target PHCs in golden pompano would not pose risks to human. Our study presents the first report of a nationwide survey of PHCs contamination in farmed golden pompano in China.


Assuntos
Contaminação de Alimentos/análise , Hidrocarbonetos Clorados/análise , Perciformes , Poluentes Químicos da Água/análise , Animais , Aquicultura , Peso Corporal , China , Produtos Pesqueiros/análise , Cromatografia Gasosa-Espectrometria de Massas , Éteres Difenil Halogenados/análise , Humanos , Nível de Efeito Adverso não Observado , Praguicidas/análise , Bifenilos Policlorados/análise
15.
Chemosphere ; 243: 125404, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31995871

RESUMO

Scombroid poisoning in fish-based and other food products has raised concerns due to toxicity outbreaks and incidences associated with histamine, thus measuring the amount of histamine toxic molecule is considered crucial quality indicator of food safety and human health. In this study, liposome-based measurement of histamine was performed via rupturing mechanism of sulforhodamine B dye encapsulated anti-histamine antibody conjugated liposomal nanovesicles. The immunosensing ability of immuno-liposomal format was assessed by monitoring the fluorescence at excitation/emission wavelength of 550/585 nm. Immuno-liposomal format assays were considered, one based on single wash procedure (Method 1), which had a detection limit of 10 ppb and quantification limit 15-80 ppb. While Method 2 based on one-by-one wash procedure had a detection limit of 2-3 ppb and quantification limit 8.5 ppb-200 ppm that required 2 h 30 min to perform. In view of better quantification limit, Method 2 was chosen for further tests required to validate its applicability in real samples. The feasibility of Method 2 was reconfirmed in fresh mackerel fish, and canned fish (tuna and salmon) with a similar detection limits but with low amplified fluorescence signals and sufficient levels of histamine recovery from fresh mackerel (73.50-99.98%), canned tuna (79.08-103.74%) and salmon (74.56-99.02%). The specificity and method accuracy were expressed as % CV in the range 5.34%-8.48%. Overall, the developed multi-well sensing system (Method 2) showed satisfactory specificity, cost effectiveness, rapidity, and stability for monitoring histamine toxicity as a practical food diagnostic device.


Assuntos
Imunofluorescência/métodos , Contaminação de Alimentos/análise , Histamina/análise , Toxinas Marinhas/análise , Toxinas Marinhas/envenenamento , Animais , Produtos Pesqueiros/análise , Peixes , Inocuidade dos Alimentos , Histamina/imunologia , Antagonistas dos Receptores Histamínicos , Humanos , Limite de Detecção , Lipossomos/imunologia , Rodaminas , Salmão , Alimentos Marinhos/análise , Sensibilidade e Especificidade , Atum
16.
Environ Geochem Health ; 42(2): 563-577, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31432347

RESUMO

This study aimed to determine the levels of metals and metalloid (arsenic) in pit lakes from a gold mining site and in their fishes and assess their potential health effect on the local human population, in order to evaluate whether pit lakes can be safely used for aquaculture. Water quality data were collected from two pit lakes, namely West Pit 1 (WP1) and West Pit 2 (WP2), and the Nakambé River (NR) in Burkina Faso. Fish consumption rates in different villages were assessed through a survey. Commonly available fish were sampled from the pit lakes and the NR. Fish from the pit lakes contained higher amounts of metals and metalloid than fish from the river (WP1 ~ WP2 < NR). Of the four species of fish considered, Oreochromis niloticus and Hydrocynus forskahlii had the highest metals and metalloid content and Bagrus bajad and Clarias anguillaris had the lowest. The results indicated that the consumption of the whole fish results in higher metals and metalloid intake than consumption of the fleshy part only. Due to the low fish intake of 5.34 ± 2.60 g/day/adult deduced from the nutritional survey, exposure to metals and metalloid was below referential doses. The highest arsenic intake comes from eating entire O. niloticus (0.058 mg/day/adult) from WP1. Eating O. niloticus and C. anguillaris exposes people to an arsenic intake of 0.01 mg/day/adult. The arsenic contents of H. forskahlii and B. bajad were below the method detection limit.


Assuntos
Exposição Dietética/análise , Peixes , Contaminação de Alimentos/análise , Lagos/análise , Metaloides/análise , Metais/análise , Animais , Burkina Faso , Exposição Dietética/efeitos adversos , Ingestão de Alimentos , Monitoramento Ambiental/métodos , Feminino , Produtos Pesqueiros/análise , Ouro , Humanos , Lagos/química , Masculino , Mineração , Medição de Risco , Rios , Poluentes Químicos da Água/análise , Qualidade da Água
17.
Environ Geochem Health ; 42(2): 531-543, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31376046

RESUMO

This study aimed to assess the effects of major ecotoxic heavy metals accumulated in the Buriganga and Turag River systems on the liver, kidney, intestine, and muscle of common edible fish species Puntius ticto, Heteropneustes fossilis, and Channa punctatus and determine the associated health risks. K was the predominant and reported as a major element. A large concentration of Zn was detected in diverse organs of the three edible fishes compared with other metals. Overall, trace metal analysis indicated that all organs (especially the liver and kidney) were under extreme threat because the maximum permissible limit set by different international health organizations was exceeded. The target hazard quotient and target cancer risk due to the trace metal content were the largest for P. ticto. Thus, excessive intake of P. ticto from the rivers Buriganga and Turag could result in chronic risks associated with long-term exposure to contaminants. Histopathological investigations revealed the first detectable indicators of infection and findings of long-term injury in cells, tissues, and organs. Histopathological changes in various tissue structures of fish functioned as key pointers of connection to pollutants, and definite infections and lesion types were established based on biotic pointers of toxic/carcinogenic effects. The analysis of histopathological alterations is a controlling integrative device used to assess pollutants in the environment.


Assuntos
Peixes , Metais Pesados/análise , Medição de Risco/métodos , Rios/química , Poluentes Químicos da Água/análise , Animais , Bangladesh , Peixes-Gato , Monitoramento Ambiental , Produtos Pesqueiros/análise , Contaminação de Alimentos , Humanos , Rim/efeitos dos fármacos , Rim/patologia , Fígado/efeitos dos fármacos , Fígado/patologia , Metais Pesados/farmacocinética , Metais Pesados/toxicidade , Músculos/química , Músculos/efeitos dos fármacos , Distribuição Tecidual , Poluentes Químicos da Água/farmacocinética , Poluentes Químicos da Água/toxicidade
18.
Food Chem ; 311: 125900, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31780223

RESUMO

Nucleotide degradation in fish is an important biochemical change after death, which is closely related to freshness and sensory quality. However, except ATP-relative nucleotides, it remains unclear about changes in other nucleotide metabolites during postmortem stage. In this study, a strategy for the simultaneous quantification of 28 nucleobases, nucleosides, and nucleotides using hydrophilic interaction chromatography coupled with tandem mass spectrometry (HILIC-MS/MS) with positive/negative ion switching was developed. This method showed good linearity, precision, repeatability, and recovery. Furthermore, it was successfully applied to monitor the postmortem nucleotide degradation of turbot mince during chill (4 °C) and partial freezing (-3 °C) storage for 168 h. It was noted that the patterns of the changes in nucleotide metabolites differed considerably depending on the storage temperature. Meanwhile, the different pathway and speed of nucleotide catabolism in turbot mince was summarized based on the quantification data.


Assuntos
Produtos Pesqueiros/análise , Músculos/metabolismo , Nucleosídeos/química , Animais , Cromatografia Líquida de Alta Pressão , Linguados/metabolismo , Congelamento , Interações Hidrofóbicas e Hidrofílicas , Músculos/química , Nucleosídeos/metabolismo , Nucleotídeos/química , Nucleotídeos/metabolismo , Espectrometria de Massas em Tandem
19.
Int J Food Microbiol ; 314: 108416, 2020 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-31707172

RESUMO

The population, diversity and succession of microbial communities and chemical characteristics of dried crimson snapper (Lutianus erythropterus) during storage for 50 days were investigated. The population of bacteria, yeasts and filamentous fungi in the samples were enumerated by culture methods using appropriate agar media. The amplicons of the 16S rRNA of bacteria and the ITS region of fungi were sequenced and compared with gene libraries to obtain the identity and abundance of microorganisms in the community. Free amino acids and several other chemical characteristics were determined by HPLC and corresponding chemical reaction methods. Before storage, the average counts of bacteria, yeasts and filamentous fungi in the dried fish were 3.2, 2.5 and 2.2 log CFU/g, which increased to 4.6, 3.6 and 3.9 log CFU/g, respectively after storage. Major succession in the bacterial > fungal communities occurred during storage as evidenced by a significant decline in the number and diversity of microbial communities. Predominant bacterial genera were Phytobacteria, Vibrio, Acinetobacter and Macrococcus in the freshly dried fish, which were replaced by Bacillaceae, Halomonas, Lentibacillus, Alkalibacillus after storage. The fungal community of the freshly dried fish consisted of Penicillum > Yamadazyma, Malassezia, Candida and Eurotiales with Penicillum being the most dominant. Penicillium camemberti was the most abundant fungal species in the dried fish before storage with most dominant after storage and accounted for >96% of the abundance. The succession in the microbial community was accompanied by major changes in chemical characteristics with a significant decrease in fat, and an increase in total free amino acids and total volatile basic nitrogen (TVB-N).


Assuntos
Produtos Pesqueiros/análise , Produtos Pesqueiros/microbiologia , Alimentos em Conserva/análise , Alimentos em Conserva/microbiologia , Microbiota , Aminoácidos/análise , Animais , Bactérias/genética , Bactérias/crescimento & desenvolvimento , Bactérias/isolamento & purificação , DNA Ribossômico/genética , Gorduras/análise , Peixes , Microbiologia de Alimentos , Fungos/genética , Fungos/crescimento & desenvolvimento , Fungos/isolamento & purificação , Microbiota/genética , Nitrogênio/análise
20.
J Sci Food Agric ; 100(3): 1195-1203, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31721226

RESUMO

BACKGROUND: The present study investigated the effect of two-step heat treatments on the structure of grass carp myofibrillar proteins (MPs) and their binding ability for selected aldehydes (hexanal, heptanal, octanal and nonanal). RESULTS: Within 30 min of the first heating step at 40 °C and 5-10 min of the second heating step at 90 °C, the enhancement of the flavor-binding ability was likely explained by the increases in surface hydrophobicity and total sulfhydryl content due to the unfolding of secondary structures of MPs through exposure of hydrophobic amino acids and sulfhydryl groups. Nevertheless, lengthy heating at 90 °C accelerated the aggregation of unfolded MPs and reduced the hydrophobic bonding sites, thus weakening the hydrophobic interactions and decreasing the resultant binding ability of MPs with aldehydes. CONCLUSION: The binding ability of aldehydes to MPs was found to be strongly influenced by changes in protein structure and surface during the two-step heating process. The results provided insight into improving the flavor characteristics of freshwater fish surimi products. © 2019 Society of Chemical Industry.


Assuntos
Aldeídos/análise , Produtos Pesqueiros/análise , Proteínas de Peixes/química , Miofibrilas/química , Animais , Carpas , Aromatizantes/química , Temperatura Alta , Interações Hidrofóbicas e Hidrofílicas , Estrutura Secundária de Proteína
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