Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 3.799
Filtrar
1.
Cell Mol Life Sci ; 77(1): 129-147, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31363816

RESUMO

Obesity is an endemic pathophysiological condition and a comorbidity associated with hypercholesterolemia, hypertension, cardiovascular disease, type 2 diabetes mellitus, and cancer. The adipose tissue of obese subjects shows hypertrophic adipocytes, adipocyte hyperplasia, and chronic low-grade inflammation. S100 proteins are Ca2+-binding proteins exclusively expressed in vertebrates in a cell-specific manner. They have been implicated in the regulation of a variety of functions acting as intracellular Ca2+ sensors transducing the Ca2+ signal and extracellular factors affecting cellular activity via ligation of a battery of membrane receptors. Certain S100 proteins, namely S100A4, the S100A8/S100A9 heterodimer and S100B, have been implicated in the pathophysiology of obesity-promoting macrophage-based inflammation via toll-like receptor 4 and/or receptor for advanced glycation end-products ligation. Also, serum levels of S100A4, S100A8/S100A9, S100A12, and S100B correlate with insulin resistance/type 2 diabetes, metabolic risk score, and fat cell size. Yet, secreted S100B appears to exert neurotrophic effects on sympathetic fibers in brown adipose tissue contributing to the larger sympathetic innervation of this latter relative to white adipose tissue. In the present review we first briefly introduce S100 proteins and then critically examine their role(s) in adipose tissue and obesity.


Assuntos
Tecido Adiposo/metabolismo , Obesidade/metabolismo , Proteínas S100/metabolismo , Tecido Adiposo/fisiopatologia , Animais , Citocinas/análise , Citocinas/metabolismo , Humanos , Inflamação/complicações , Inflamação/metabolismo , Inflamação/fisiopatologia , Macrófagos/metabolismo , Macrófagos/patologia , Obesidade/complicações , Obesidade/fisiopatologia , Proteínas S100/análise
2.
Mol Med Rep ; 20(3): 2476-2483, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31322196

RESUMO

Atopic dermatitis (AD) is an inflammatory skin disorder caused by immunological dysregulation and genetic factors. Whether the expression levels of cytokine and skin barrier protein were altered by S100 calcium binding protein A8 (S100A8) and S100A9 in human keratinocytic HaCaT cells was examined in the present study. Alterations of cytokine expression were examined by ELISA following treatment with S100A8/9 and various signal protein­specific inhibitors. Activation of the mitogen activated protein kinase (MAPK) pathway and nuclear factor (NF)­κB was evaluated by using western blotting and an NF­κB activity test, respectively. The expression levels of interleukin (IL)­6, IL­8 and monocyte chemoattractant protein­1 increased following treatment with S100A8 and S100A9, and the increase was significantly blocked by specific signaling pathway inhibitors, including toll­like receptor 4 inhibitor (TLR4i), rottlerin, PD98059, SB203580 and BAY­11­7085. Extracellular signal­regulated kinase (ERK) and p38 MAPK pathways were activated in a time­dependent manner following treatment with S100A8 and S100A9. Phosphorylation of ERK and p38 MAPK were blocked by TLR4i and rottlerin. S100A8 and S100A9 induced translocation of NF­κB in a time­dependent manner, and the activation of NF­κB was inhibited by TLR4i, rottlerin, PD98059 and SB203580. In addition, S100A8 and S100A9 decreased the expression of skin barrier proteins, filaggrin and loricrin. These results may help to elucidate the pathogenic mechanisms of AD and develop clinical strategies for controlling AD.


Assuntos
Calgranulina A/imunologia , Calgranulina B/imunologia , Citocinas/imunologia , Queratinócitos/imunologia , Proteínas de Membrana/imunologia , Proteínas S100/imunologia , Linhagem Celular , Citocinas/análise , Dermatite Atópica/imunologia , Humanos , Sistema de Sinalização das MAP Quinases , Proteínas de Membrana/análise , NF-kappa B/análise , NF-kappa B/imunologia , Proteínas S100/análise
3.
Cell Physiol Biochem ; 53(1): 76-86, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31192545

RESUMO

BACKGROUND/AIMS: Diabetes causes damage to the enteric nervous system. The enteric nervous system consists of neurons and enteric glial cells (EGCs). The present study evaluated the effects of an ethyl-acetate fraction (EAF) from Trichilia catigua (T. catigua; 200 mg/kg) on the total population of enteric neurons (HuC/D-immunoreactive [IR]) and EGCs (S100-IR and glial fibrillary acidic protein [GFAP]-IR) in the total preparation and jejunal mucosa in diabetic rats. METHODS: The animals were distributed into four groups: normoglycemic rats (N), diabetic rats (D), normoglycemic rats that received the EAF (NC), and diabetic rats that received the EAF (DC). The jejunum was processed for immunohistochemistry to evaluate HuC/D, S100, and GFAP immunoreactivity. The expression of S100 and GFAP proteins was also quantified by Western blot. RESULTS: The D group exhibited a decrease in the number of neurons and EGCs, an increase in the area of cell bodies, an increase in S100 protein expression, a decrease in GFAP protein expression, and a decrease in S100-IR and GFAP-IR EGCs in the jejunal mucosa. The DC group exhibited a decrease in the number of neurons and EGCs, a decrease in the area of cell bodies, a decrease in S100 and GFAP protein expression, and a decrease in S100-IR and GFAP-IR EGCs in the jejunal mucosa. The NC group exhibited maintenance of the number of neurons and EGCs, an increase in the area of cell bodies, and a decrease in S100 and GFAP protein expression. CONCLUSION: The EAF from T. catigua partially conferred protection against diabetic neuropathy in the enteric nervous system.


Assuntos
Diabetes Mellitus Experimental/complicações , Neuropatias Diabéticas/prevenção & controle , Jejuno/inervação , Meliaceae/química , Neuroglia/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Fármacos Neuroprotetores/farmacologia , Extratos Vegetais/farmacologia , Acetatos/química , Animais , Diabetes Mellitus Experimental/patologia , Neuropatias Diabéticas/etiologia , Neuropatias Diabéticas/patologia , Sistema Nervoso Entérico/efeitos dos fármacos , Proteína Glial Fibrilar Ácida/análise , Jejuno/efeitos dos fármacos , Jejuno/patologia , Masculino , Neuroglia/patologia , Neurônios/patologia , Neuroproteção/efeitos dos fármacos , Fármacos Neuroprotetores/química , Fármacos Neuroprotetores/uso terapêutico , Extratos Vegetais/química , Extratos Vegetais/uso terapêutico , Ratos , Ratos Wistar , Proteínas S100/análise
4.
Ginekol Pol ; 90(4): 223-227, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31059116

RESUMO

Ovarian cancer is the fifth leading cause of cancer-related deaths in women. Its high mortality rate results from lack of adequate and sensitive methods allowing for the detection of the early stages of the disease, as well as low efficiency of the treatment, caused by the cytotoxic drug resistance of cancer cells. Unfortunately, tumours are able to develop new pathways and protective mechanisms that allow them to survive toxic conditions of chemotherapy. Therefore, intensive search for new genes and proteins involved in resistance to cytotoxic drugs is still needed, especially from a clinical point of view. The article presents an overview of the available literature on the role of semaphorin 3A (SEMA3A), protocadherin 9 (PCDH9), and S100 calcium binding protein A3 (S100A3) in carcinogenesis and chemoresistance of various tumors including ovarian cancer. As it turns out, the role of described genes/proteins is not limited only to their native biological activity but they function also as an oncogenic or suppressor factors in the tumor development. Moreover, they can also play an important role in development of drug resistance, as it was shown in ovarian cancer cell lines.


Assuntos
Biomarcadores Tumorais , Caderinas , Resistencia a Medicamentos Antineoplásicos , Neoplasias Ovarianas , Proteínas S100 , Semaforina-3A , Antineoplásicos , Biomarcadores Tumorais/análise , Biomarcadores Tumorais/metabolismo , Caderinas/análise , Caderinas/metabolismo , Feminino , Humanos , Neoplasias/diagnóstico por imagem , Neoplasias/metabolismo , Neoplasias Ovarianas/diagnóstico , Neoplasias Ovarianas/metabolismo , Proteínas S100/análise , Proteínas S100/metabolismo , Semaforina-3A/análise , Semaforina-3A/metabolismo
5.
J Clin Pathol ; 72(8): 562-565, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31097491

RESUMO

AIMS: This case series intends to expand currently limited knowledge regarding the existence and diagnostic significance of intramucosal fat in colorectal polyps. METHODS: Clinicopathological features of nine such polyps were reported following histopathological review, including S100 and EMA immunohistochemistry. RESULTS AND CONCLUSIONS: Such review subdivided seven polyps into three groups: (1) mucosal perineurioma/serrated polyps with fat among the perineurial stroma (three cases); (2) submucosal lipomas with adipose tissue extending into the overlying mucosa (two cases) and (3) polyps with intramucosal adipose tissue only, that is, the newly described but less-recognised entity known as intramucosal lipoma (two cases). The two remaining polyps of this series did not include submucosa but, from assessing their muscularis mucosae, were favoured to represent intramucosal lipomas. The first two phenomena are formally described for the first time by this case series. The last of these three diagnoses should prompt investigations for Cowden syndrome, but intramucosal lipomas are more often sporadic/non-syndromic.


Assuntos
Tecido Adiposo/patologia , Pólipos do Colo/patologia , Neoplasias Colorretais/patologia , Mucosa Intestinal/patologia , Pólipos Intestinais/patologia , Lipoma/patologia , Doenças Retais/patologia , Idoso , Pólipos do Colo/química , Neoplasias Colorretais/química , Diagnóstico Diferencial , Feminino , Humanos , Imuno-Histoquímica , Mucosa Intestinal/química , Pólipos Intestinais/química , Lipoma/química , Masculino , Pessoa de Meia-Idade , Mucina-1/análise , Valor Preditivo dos Testes , Doenças Retais/metabolismo , Proteínas S100/análise
6.
Endocr Pathol ; 30(2): 90-95, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31001800

RESUMO

We describe a consistently present, previously unrecognized, population of monocytes in pheochromocytomas and paragangliomas. Although sustentacular cells are generally recognized as a common component of these tumors, differential immunohistochemical staining for CD163 and S100 shows that monocytes can in fact be more numerous. These cells frequently resemble sustentacular cells topographically and cytologically, possibly explaining why they have not been previously noticed. They contribute to the tumor proteome and may have implications for tumor biology. No correlations were identifiable between the presence of these cells and any clinical characteristics of the tumors in the present study. A possible association with genotype is suggested by immunoblot showing high expression of CD163 protein in tumors with succinate dehydrogenase mutations.


Assuntos
Neoplasias das Glândulas Suprarrenais/patologia , Monócitos/patologia , Paraganglioma/patologia , Feocromocitoma/patologia , Neoplasias das Glândulas Suprarrenais/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Antígenos CD/análise , Antígenos de Diferenciação Mielomonocítica/análise , Feminino , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Mutação/genética , Paraganglioma/genética , Feocromocitoma/genética , Receptores de Superfície Celular/análise , Proteínas S100/análise , Succinato Desidrogenase/genética
7.
Ann Anat ; 224: 62-72, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31005573

RESUMO

Pacinian corpuscles are onion bulb-like multilayered mechanoreceptors that consist of a complicated structure of axon terminals, Schwann related cells (inner core), endoneural related cells (intermediate layer) and perineurial related cells (outer core-capsule). The cells forming those compartments are continuous and share the properties of that covering the nerve fibers. Small leucine-rich proteoglycans are major proteoglycans of the extracellular matrix and regulate collagen fibrillogenesis, cell signalling pathways and extracellular matrix assembly. Here we used immunohistochemistry to investigate the distribution of class I (biglycan, decorin, asporin, ECM2 and ECMX) and class II (fibromodulin, lumican, prolargin, keratocan and osteoadherin) small leucine-rich proteoglycans in human cutaneous Pacinian corpuscles. The distribution of these compounds was: the inner core express decorin, biglycan, lumican, fibromodulin, osteoadherin; the intermediate layer display immunoreactivity for osteoadherin; the outer core biglycan, decorin, lumican, fibromodulin and osteoadherin; and the capsule contains biglycan, decorin, fibromodulin, and lumican. Asporin, prolargin and keratocan were undetectable. These results complement our knowledge about the distribution of small leucine-rich proteoglycans in human Pacinian corpuscles, and help to understand the composition of the extracellular matrix in these sensory formations.


Assuntos
Corpúsculos de Pacini/química , Proteoglicanas/análise , Adolescente , Adulto , Animais , Antígenos CD34/análise , Biglicano/análise , Criança , Decorina/análise , Equidae , Proteínas da Matriz Extracelular/análise , Fibromodulina/análise , Dedos , Imunofluorescência , Cabras , Humanos , Imuno-Histoquímica , Camundongos , Pessoa de Meia-Idade , Proteoglicanas/classificação , Coelhos , Proteínas S100/análise , Pele/anatomia & histologia , Vimentina/análise , Adulto Jovem
8.
Am J Clin Pathol ; 151(6): 574-583, 2019 05 03.
Artigo em Inglês | MEDLINE | ID: mdl-30895287

RESUMO

OBJECTIVES: Perineural invasion (PNI) is an important factor in tumor prognosis. We evaluated p75 nerve growth factor receptor (p75NGFR) as a neuromarker for perineural invasion of squamous cell carcinoma. METHODS: A comparison of H&E, and S100 and p75NGFR immunohistochemical staining methods, using sequential sections from 29 samples, for identification nerve bundles and PNI diagnosis, was carried out. RESULTS: p75NGFR and S100 correctly identified more nerve bundles than H&E. Accuracy of p75NGFR was higher than that of S100, but there was no significant difference from H&E. The accuracy of nerve bundle identification by p75NGFR and S100 showed no significant difference among different histopathologic grades of squamous cell carcinomas. p75NGFR gave mild staining of small vessels, while S100 gave strong staining of smooth muscle and glandular tissue. CONCLUSIONS: p75NGFR specifically stains nerve tissue and is better than S100 and H&E in identifying nerve bundles. p75NGFR is expected to become a new neuromarker.


Assuntos
Carcinoma de Células Escamosas/patologia , Períneo/patologia , Receptor de Fator de Crescimento Neural/análise , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores , Carcinoma de Células Escamosas/química , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica , Estadiamento de Neoplasias , Tecido Nervoso/química , Proteínas S100/análise
9.
Ann Dermatol Venereol ; 146(5): 372-376, 2019 May.
Artigo em Francês | MEDLINE | ID: mdl-30910333

RESUMO

BACKGROUND: Myxoid melanoma is a rare variant of melanoma that must be recognised. Herein we describe a new metastatic case. PATIENTS AND METHODS: A 78-year-old woman consulted for a firm, pinkish nodule measuring 25mm and present for six months on her left leg. Analysis of the biopsy revealed achromic fusiform tumour cells separated by large myxoid plaques. Labeling of SOX10, HMB45 and PS100 was diffuse and of moderate to strong intensity. A diagnosis of myxoid melanoma was considered, with Breslow thickness of 9mm. Surgery was carried out with a 2-cm margin and confirmed the diagnosis. Dermatological follow-up at one year revealed metastatic spread to the ganglia, pleura, liver and bone. DISCUSSION: Few cases of primary myxoid melanoma have been described, and the condition is probably underdiagnosed. The classic clinical presentation of this condition consists of a solitary achromic nodule found chiefly on the limbs. The microscopic appearance is relatively non-specific. Immunohistochemical analysis may indicate melanocytic involvement: cells exhibit expression of SOX10, diffuse expression of protein S100, and less consistent and more variable expression of HMB45. The increasingly common use of anti-SOX10 is of value since it is expressed in the nucleus of melanocytes. Mastocytes and TGF-ß secretion appear to be involved in myxoid stroma production. In the absence of specific codification, management of myxoid melanoma is comparable to that of other types of melanoma. There is uncertainty about the prognosis, with the involvement of TGF-ß possibly indicating the aggressive potential of this type of tumour.


Assuntos
Perna (Membro) , Melanoma/patologia , Neoplasias Cutâneas/patologia , Idoso , Biomarcadores Tumorais/análise , Biópsia , Feminino , Humanos , Melanócitos/química , Melanoma/química , Antígenos Específicos de Melanoma/análise , Proteínas S100/análise , Fatores de Transcrição SOXE/análise , Neoplasias Cutâneas/química
10.
Int J Surg Pathol ; 27(5): 541-546, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30773953

RESUMO

IgG4-related disease is a recent entity that has been described in a wide variety of organ systems. A 46-year-old female presented with acute appendicitis accompanied by a mass-forming lesion, raising a concern for neoplasm, and therefore, hemicolectomy was performed. The lesion revealed a dense lymphoplasmacytic infiltrate accompanied by storiform fibrosis and obliterative phlebitis. The IgG4/IgG plasma cell ratio was >50%, and the number of IgG4-positive plasma cells was >100/high-power field. In order to assess the IgG4/IgG plasma cell ratio, MUM1 was employed instead of IgG to successfully estimate the plasma cell concentration. There was also a concomitant hyperplasia of S100-positive cell, which could represent dendritic or Schwannian origin and possibly play a pathophysiologic role. The hyperplasia was significant by itself that it may mimic a mass-forming lesion. This newly described entity of the past decade deserves increased recognition due to clinical implication and surgical morbidity. This is the first case of IgG4-related disease in the appendix to our knowledge that fully satisfied all the pathological diagnostic criteria. We would like to also highlight our innovative approach of evaluating the IgG4/IgG plasma cell.


Assuntos
Apêndice/patologia , Doenças do Ceco/diagnóstico , Doença Relacionada a Imunoglobulina G4/diagnóstico , Apêndice/citologia , Apêndice/cirurgia , Doenças do Ceco/patologia , Doenças do Ceco/cirurgia , Colectomia , Células Dendríticas/patologia , Diagnóstico Diferencial , Feminino , Humanos , Hiperplasia/patologia , Doença Relacionada a Imunoglobulina G4/patologia , Doença Relacionada a Imunoglobulina G4/cirurgia , Mucosa Intestinal/patologia , Mucosa Intestinal/cirurgia , Pessoa de Meia-Idade , Proteínas S100/análise , Células de Schwann/patologia
11.
Methods Mol Biol ; 1929: 663-678, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30710303

RESUMO

The S100 protein family has attracted great interest in the field of biomarker research, and a growing number of studies reveal dysregulation of many of the 21 S100 protein isoforms in various human diseases. In cancer, S100 protein expression has been associated with tumor growth, progression, and response to treatment. Some S100 proteins are also considered candidate therapeutic targets. From an analytical perspective, multiplexed analysis of the family-wide S100 protein expression is challenging due to their relatively small size and high-sequence identity. Here we describe a mass spectrometry method using selected reaction monitoring which enables the targeted, multiplexed detection and quantitation of the entire S100 protein family in cell lines and tissue samples.


Assuntos
Neoplasias/metabolismo , Proteômica/métodos , Proteínas S100/análise , Biomarcadores Tumorais/análise , Biomarcadores Tumorais/química , Linhagem Celular Tumoral , Cromatografia Líquida , Motivos EF Hand , Regulação Neoplásica da Expressão Gênica , Humanos , Espectrometria de Massas/métodos , Peso Molecular , Proteínas S100/química
13.
Clin Transl Oncol ; 21(2): 220-231, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29956073

RESUMO

PURPOSE: The aim of this study is to explore the roles of ß-catenin, decorin, septin-7, and S100A10 expression in colorectal cancer development. METHODS: Twenty-five BALB/c mice were divided into five groups; four groups were administrated N,N-dimethylhydrazine for 0, 10, 15, and 20 weeks, and one group was administrated normal saline for 20 weeks. The colons were collected for histopathological analysis. Protein samples prepared from the frozen colon tissues of mice treated with N,N-dimethylhydrazine for the different time points were evaluated using the isobaric tags for relative and absolute quantification (iTRAQ) labeling technique coupled with the 2D liquid chromatography-tandem mass spectrometry analysis. Based on the proteomic analysis results, immunohistochemical staining of ß-catenin, decorin, septin-7, and S100A10 was performed in paraffin-embedded mice colorectal tissue, and 53 cases of human hereditary polyposis colorectal cancer samples. RESULTS: Colorectal cancer was observed in mice treated with N,N-dimethylhydrazine for 20 weeks, and adenomas were observed in mice subjected to the 10-, and 15-week treatments. Seventy-two differentially expressed proteins were involved in the development of cancer as per the iTRAQ and spectrometry analysis. In normal epithelium, adenoma, and cancer from human hereditary polyposis colorectal cancer, S100A10 expression (c2 = 100.989, P = 0.000) was highest in cancer, whereas decorin (c2 = 12.852, P = 0.002) and septin-7 (c2 = 66.519, P = 0.002) expressions were highest in the normal epithelium, which was confirmed via immunohistochemical staining. CONCLUSIONS: The subcellular localization of ß-catenin and decorin, septin-7, and S100A10 expressions are associated with the development of colorectal cancer in mice after N,N-dimethylhydrazine treatment and in human hereditary polyposis colorectal cancers.


Assuntos
Polipose Adenomatosa do Colo/patologia , Biomarcadores Tumorais/análise , Neoplasias Colorretais/patologia , Adulto , Animais , Anexina A2/análise , Anexina A2/biossíntese , Carcinógenos/toxicidade , Neoplasias Colorretais/induzido quimicamente , Decorina/análise , Decorina/biossíntese , Dimetilidrazinas/toxicidade , Feminino , Humanos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Pessoa de Meia-Idade , Proteômica/métodos , Proteínas S100/análise , Proteínas S100/biossíntese , Septinas/análise , Septinas/biossíntese , beta Catenina/análise , beta Catenina/biossíntese
15.
Photochem Photobiol Sci ; 18(1): 129-139, 2019 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-30357250

RESUMO

Although infrared radiation (IR) represents more than 50% of the solar radiation reaching the Earth's surface, this waveband has been hardly investigated in terms of tumourigenesis. The objective of the present study was to investigate the influence of IR on ultraviolet B (UVB)-induced carcinogenesis in male and female wild type mice. For this purpose, male and female C57BL/6N mice were subjected to a long-term irradiation protocol. Mice were irradiated once neonatally and from the age of eight weeks for 36 weeks with a cumulative dose of 576 kJ m-2 UVB and/or 78 895 kJ m-2 IR. In order to resemble natural sun irradiation, exposure to physiological doses of UVB and IR was performed simultaneously. Mice were screened for arising lesions twice a week. Lesions were excised and histologically diagnosed. Kaplan-Meier analyses were carried out and lesion counts and cumulated hazard rates for the development of lesions in the UVB and IR + UVB-exposed groups in male and female mice were compared. We found that IR-exposure did not change the number of epithelial malignant tumours in UVB-exposed wild type mice. In combination with IR there was a tendency of more tumours with increased malignancy: 23 vs. seven spindle cell shaped sarcomas and seven vs. two MelanA+/S100+ tumours in groups of 35 C57BL/6 mice. IR did not influence UVB-induced carcinogenesis differently in male and female mice. However, comparing UVB and sham irradiated animals irrespective of IR exposure, UVB-induced non-epithelial tumours arose significantly earlier in male mice than in female mice.


Assuntos
Carcinogênese/efeitos da radiação , Raios Infravermelhos/efeitos adversos , Neoplasias Induzidas por Radiação/etiologia , Sarcoma Experimental/etiologia , Neoplasias Cutâneas/etiologia , Pele/efeitos da radiação , Raios Ultravioleta/efeitos adversos , Animais , Feminino , Humanos , Estimativa de Kaplan-Meier , Antígeno MART-1/análise , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Proteínas S100/análise , Sarcoma Experimental/patologia , Fatores Sexuais , Pele/patologia , Neoplasias Cutâneas/patologia
16.
Int J Surg Pathol ; 27(5): 515-517, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30541360

RESUMO

In this article, we report the case of a 40-year-old woman with a sessile polyp of the rectosigmoid junction that underwent endoscopic resection. The resected specimen showed spindle cell proliferation with eosinophilic cytoplasm between mucosal crypts, mimicking mucosal prolapse syndrome. However, these were immunohistochemically positive for S-100, indicating neural cell origin. As neural polypoid lesion of the rectum, neurofibroma, perineurioma, schwannoma, and mucosal Schwann cell hamartoma were in the differential diagnosis. Histology and additional immunohistochemistry confirmed mucosal Schwann cell hamartoma. Mucosal Schwann cell hamartoma of the rectosigmoid junction or rectum can be a histological mimic of mucosal prolapse syndrome and other S-100 positive neural cell lesions; however, the lesion in the present case was correctly diagnosed with histology and immunohistochemistry.


Assuntos
Hamartoma/diagnóstico , Mucosa Intestinal/patologia , Pólipos Intestinais/diagnóstico , Células de Schwann/patologia , Adulto , Colo Sigmoide/patologia , Colo Sigmoide/cirurgia , Colonoscopia , Diagnóstico Diferencial , Feminino , Hamartoma/patologia , Hamartoma/cirurgia , Humanos , Mucosa Intestinal/cirurgia , Pólipos Intestinais/patologia , Pólipos Intestinais/cirurgia , Reto/patologia , Reto/cirurgia , Proteínas S100/análise
17.
Balkan Med J ; 36(2): 106-112, 2019 02 28.
Artigo em Inglês | MEDLINE | ID: mdl-30396875

RESUMO

Background: Although water-pipe smoking is a great public health problem, data regarding the acute and chronic effects and the degree of toxin exposure are limited. While water pipe-related malignancy, pulmonary, infectious, cardiac effects, infertility, and biological effects have been described in a meta-analysis, there are no studies in the literature about its neurologic effects. Aims: To evaluate water pipe-related acute neurological effects and cerebral blood flow through transcranial Doppler ultrasonography and serum S100 calcium binding protein calcium binding protein level measurements. Study Design: Prospective observational study. Methods: Vital signs and baseline carboxyhemoglobin and S100 calcium binding protein levels, cerebral flood changes with transcranial Doppler ultrasound were evaluated and recorded before and after water-pipe smoking. Results: The mean age of the 31 volunteers was 30.61 (±5.67) years, and 24 of them (77.42%) were male. A statistically significant difference was determined in heart rate, oxygen saturation, systolic and diastolic arterial pressure values before and after water-pipe smoking (p<0.001, p=0.035, p=0.009, p=0.021, respectively). Mean carboxyhemoglobin level was 2.68% (±1.68) before, 14.97% (±4.83) after water-pipe smoking (p<0.001). The S100 calcium binding protein level was 25.05 µ/mL (±8.34) at the beginning, 40.71 µ/mL (±14.06) after water-pipe smoking (p<0.001). An increase was determined in peak, and median middle, anterior and posterior cerebral artery blood flow rates, and a decrease was determined in both the pulsatility index and resistivity index values after water-pipe smoking using transcranial Doppler ultrasound. Conclusion: Cerebral vasodilation develops due to the increase in cerebral blood flow rate and the decrease in pulsatility index, resistivity index values, and the elevation in carboxyhemoglobin, S100 calcium binding protein level indicates that water-pipe smoking leads to neuronal damage in the acute period.


Assuntos
Circulação Cerebrovascular/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Fumar Cachimbo de Água/efeitos adversos , Adulto , Carboxihemoglobina/análise , Circulação Cerebrovascular/fisiologia , Distribuição de Qui-Quadrado , Feminino , Humanos , Masculino , Estudos Prospectivos , Proteínas S100/análise , Proteínas S100/sangue , Ultrassonografia Doppler/métodos
18.
J Craniofac Surg ; 30(1): e19-e21, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30444783

RESUMO

Fibromyxomas are rare tumors that are not commonly seen in the orbit. The authors present a case of orbital fibromyxoma. A 42-year-old male presented with proptosis of the right eye that began 1 month prior. Magnetic resonance imaging revealed the presence of an orbital mass in the right eye, with low signal intensity on T1-weighted images and heterogeneous high-signal intensity on T2-weighted images. The patient underwent excisional biopsy of the orbital tumor through a Krönlein approach. The tumor originated from the inferior rectus muscle. Histopathologic analysis demonstrated scattered spindle cells, with both fibrous and myxoid stroma. Immunohistochemical staining was positive for Vimentin and negative for S-100 protein and CD34. The tumor was diagnosed as orbital fibromyxoma. The patient showed no evidence of recurrence over 18 months of follow-up after operation.


Assuntos
Fibroma/cirurgia , Neoplasias Orbitárias/cirurgia , Adulto , Exoftalmia/etiologia , Fibroma/diagnóstico por imagem , Humanos , Imagem por Ressonância Magnética , Masculino , Músculos Oculomotores , Neoplasias Orbitárias/diagnóstico por imagem , Proteínas S100/análise , Vimentina/análise
19.
Asian Cardiovasc Thorac Ann ; 27(2): 132-134, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30509136

RESUMO

Rosai-Dorfman disease or sinus histiocytosis is a nonmalignant disease chiefly presenting as a painless cervical lymphadenopathy, although extranodal involvement is also frequent. Involvement of the tracheobronchial tree is extremely rare, and there are no clear guidelines on management. Rosai-Dorfman disease usually presents as a tracheal tumor, although it may also present with extraluminal compression. Only 12 cases have been reported in the English language literature. Histopathology is required to confirm the diagnosis. We describe what we think is India's first reported case of histologically proven tracheal Rosai-Dorfman disease in a 40-year-old otherwise healthy lady.


Assuntos
Histiócitos/patologia , Histiocitose Sinusal/patologia , Doenças da Traqueia/patologia , Adulto , Biomarcadores/análise , Biópsia , Broncoscopia , Feminino , Histiócitos/química , Histiocitose Sinusal/diagnóstico por imagem , Histiocitose Sinusal/cirurgia , Humanos , Imuno-Histoquímica , Proteínas S100/análise , Doenças da Traqueia/diagnóstico por imagem , Doenças da Traqueia/cirurgia , Resultado do Tratamento
20.
Biomed Pharmacother ; 108: 1365-1375, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30372839

RESUMO

BACKGROUND: Spinal cord injury is a considerable health impact accompanied with physical, psychological and economic burden. Bone marrow derived mesenchymal stromal cells (BM-MSCs) transplantation was found to produce neuronal regenerative effects. Schwann-like cells differentiated from BM-MSCs have myelin-forming ability. AIM OF THE WORK: To compare the ability of BM-MSCs versus Schwann like cells to promote recovery of spinal cord injury. MATERIAL AND METHODS: Adult male albino rats were used throughout the study. BM-MSCs were harvested from femora of rats. Sciatic nerves were extracted and used in the preparation of the induction culture medium for differentiation of BM-MSCs into Schwann-like cells. Rats were divided into control, spinal cord injured (SCI), spinal cord injured plus BM-MSCs transplantation (BM-MSC) and spinal cord injured plus Schwann-like cells transplantation (Sn) groups. BBB scale assessment was performed before and after SCI in all rats. Rats were euthanized at the end of the 7th week and spinal cords were dissected and processed for light and transmission electron microscopic examinations. RESULTS: Spinal cord sections of SCI group revealed cavitation, necrosis and demyelination. BM-MSC and Sn groups showed both functional and structural improvement compared to SCI group with better BBB score and histopathological features in the BM-MSC group and more expression of S100 in the Sn group. CONCLUSION: Transplantation of BM-MSCs and Schwann-like cells improved the structural and functional alterations of spinal cord injury with better improvement in BM-MSC group.


Assuntos
Transplante de Células-Tronco Mesenquimais , Células de Schwann/transplante , Traumatismos da Medula Espinal/terapia , Animais , Diferenciação Celular , Proteína Glial Fibrilar Ácida/análise , Masculino , Células-Tronco Mesenquimais/citologia , Ratos , Ratos Sprague-Dawley , Proteínas S100/análise , Células de Schwann/citologia , Medula Espinal/patologia , Medula Espinal/fisiopatologia , Medula Espinal/ultraestrutura , Traumatismos da Medula Espinal/fisiopatologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA