Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 3.953
Filtrar
1.
J Plant Physiol ; 264: 153483, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34371311

RESUMO

Tomato plants are susceptible to drought stress, but the mechanism involved in this process still remains poorly understood. In the present study, we demonstrated that SlNAC6, a nuclear-localized protein induced by exogenous abscisic acid (ABA) or polyethylene glycol (PEG) stress treatment, plays a positive role in tomato plant response to PEG stress. Down-regulation of SlNAC6 (SlNAC6-RNAi) resulted in a semidwarf phenotype, and the SlNAC6-RNAi lines showed reduced tolerance to PEG stress, exhibiting a higher water loss rate and degree of oxidative damage, as well as lower values of proline content and antioxidant enzyme activity, when compared with those in wild type (WT). In contrast, overexpression of SlNAC6 (SlNAC6-OE) leads to a significant delay of growth, and the SlNAC6-OE lines showed greatly enhanced tolerance to PEG stress concomitant with a lower water loss rate and degree of oxidative damage, as well as higher values of proline content and antioxidant enzyme activity. Further study showed that the transcription level of ABA signaling-related genes and the ABA content are respectively decreased or increased in SlNAC6-RNAi and SlNAC6-OE seedlings, as verified by multiple physiological parameters, such as stomatal conductance, water loss rate, seed germination, and root length. Moreover, overexpression of SlNAC6 can accelerate tomato fruit ripening. Collectively, this study demonstrates SlNAC6 exerts important roles in tomato development, drought stress response, and fruit ripening processes, some of them perhaps partly through modulating an ABA-mediated pathway, which implies SlNAC6 may hold the potential applications in improving agronomic traits of tomato or other Solanaceae crops.


Assuntos
Lycopersicon esculentum/metabolismo , Proteínas de Plantas/fisiologia , Fatores de Transcrição/fisiologia , Desidratação , Regulação da Expressão Gênica de Plantas , Lycopersicon esculentum/genética , Lycopersicon esculentum/fisiologia , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , Reprodução , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
2.
Int J Mol Sci ; 22(16)2021 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-34445331

RESUMO

Plant WRKY transcription factors play crucial roles in plant growth and development, as well as plant responses to biotic and abiotic stresses. In this study, we identified and characterized a WRKY transcription factor in rice, OsWRKY50. OsWRKY50 functions as a transcriptional repressor in the nucleus. The transcription of OsWRKY50 was repressed under salt stress conditions, but activated after abscisic acid (ABA) treatment. OsWRKY50-overexpression (OsWRKY50-OX) plants displayed increased tolerance to salt stress compared to wild type and control plants. The expression of OsLEA3, OsRAB21, OsHKT1;5, and OsP5CS1 in OsWRKY50-OX were much higher than wild type and control plants under salt stress. Furthermore, OsWRKY50-OX displayed hyposensitivity to ABA-regulated seed germination and seedling establishment. The protoplast-based transient expression system and yeast hybrid assay demonstrated that OsWRKY50 directly binds to the promoter of OsNCED5, and thus further inhibits its transcription. Taken together, our results demonstrate that rice transcription repressor OsWRKY50 mediates ABA-dependent seed germination and seedling growth and enhances salt stress tolerance via an ABA-independent pathway.


Assuntos
Ácido Abscísico/farmacologia , Oryza , Tolerância ao Sal , Fatores de Transcrição/fisiologia , Proteínas de Arabidopsis/genética , Clonagem Molecular , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Germinação/efeitos dos fármacos , Germinação/genética , Oryza/efeitos dos fármacos , Oryza/genética , Oryza/crescimento & desenvolvimento , Filogenia , Desenvolvimento Vegetal/efeitos dos fármacos , Desenvolvimento Vegetal/genética , Proteínas de Plantas/genética , Proteínas de Plantas/fisiologia , Plantas Geneticamente Modificadas , Estresse Salino/efeitos dos fármacos , Estresse Salino/genética , Tolerância ao Sal/efeitos dos fármacos , Tolerância ao Sal/genética , Plântula/efeitos dos fármacos , Plântula/genética , Plântula/crescimento & desenvolvimento , Análise de Sequência de DNA , Homologia de Sequência , Fatores de Transcrição/genética
3.
Plant Physiol Biochem ; 166: 1087-1095, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34303268

RESUMO

Lily (Lilium spp.), with its beautiful flower, is an important horticultural crop and a popular ornamental plant, but because the abundant pollen pollutes the flowers and surroundings, its use is restricted. To solve this problem, the mechanism of pollen development in lily needs to be analyzed. However, the complex and delicate process of anther development in lily remains largely unknown. In this study, LoUDT1, a bHLH transcription factor (TF), was isolated and identified in lily. LoUDT1 was closely related to OsUDT1 of Oryza sativa and AtDYT1 of Arabidopsis. It was localized in the cytoplasm and nucleus and showed no transcriptional activation in yeast cells. LoUDT1 interacted with another bHLH TF, LoAMS, and the interaction depended on their BIF domains. LoUDT1 and LoAMS were both expressed in the anthers but showed different expression patterns. LoUDT1 was continuously expressed during the entire development of anthers, whereas LoAMS was only highly expressed early in anther development. With overexpression of LoUDT1 in Arabidopsis, normal anther development was affected and defective pollens were produced, which caused partial male sterility of transgenic plants. These defects depended on the level of LoUDT1 accumulation. By contrast, with the appropriate expression of LoUDT1 in a dyt1-3 mutant, normal pollen grains were produced, showing partial fertility. Thus, LoUDT1 might be a key regulator of anther development in lily. By further increasing the understanding of anther development, the results of this study can provide a theoretical basis for the molecular breeding of pollen-free lilies.


Assuntos
Fatores de Transcrição Hélice-Alça-Hélice Básicos , Flores/crescimento & desenvolvimento , Lilium , Proteínas de Plantas , Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Fatores de Transcrição Hélice-Alça-Hélice Básicos/fisiologia , Flores/genética , Regulação da Expressão Gênica de Plantas , Lilium/genética , Lilium/fisiologia , Proteínas de Plantas/genética , Proteínas de Plantas/fisiologia
4.
Nat Commun ; 12(1): 4470, 2021 07 22.
Artigo em Inglês | MEDLINE | ID: mdl-34294690

RESUMO

Gravity is a critical environmental factor regulating directional growth and morphogenesis in plants, and gravitropism is the process by which plants perceive and respond to the gravity vector. The cytoskeleton is proposed to play important roles in gravitropism, but the underlying mechanisms are obscure. Here we use genetic screening in Physcomitrella patens, to identify a locus GTRC, that when mutated, reverses the direction of protonemal gravitropism. GTRC encodes a processive minus-end-directed KCHb kinesin, and its N-terminal, C-terminal and motor domains are all essential for transducing the gravity signal. Chimeric analysis between GTRC/KCHb and KCHa reveal a unique role for the N-terminus of GTRC in gravitropism. Further study shows that gravity-triggered normal asymmetric distribution of actin filaments in the tip of protonema is dependent on GTRC. Thus, our work identifies a microtubule-based cellular motor that determines the direction of plant gravitropism via mediating the asymmetric distribution of actin filaments.


Assuntos
Bryopsida/fisiologia , Gravitropismo/fisiologia , Cinesina/fisiologia , Proteínas de Plantas/fisiologia , Citoesqueleto de Actina/química , Citoesqueleto de Actina/fisiologia , Sequência de Bases , Bryopsida/genética , Mapeamento Cromossômico , Citoesqueleto/química , Citoesqueleto/fisiologia , DNA de Plantas/genética , Genes de Plantas , Gravitropismo/genética , Cinesina/química , Cinesina/genética , Microtúbulos/química , Microtúbulos/fisiologia , Mutagênese , Mutação , Proteínas de Plantas/química , Proteínas de Plantas/genética , Plantas Geneticamente Modificadas , Domínios Proteicos
5.
Int J Mol Sci ; 22(13)2021 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-34201749

RESUMO

Plant abiotic stress responses are tightly regulated by different players at multiple levels. At transcriptional or post-transcriptional levels, several RNA binding proteins (RBPs) regulate stress response genes through RNA metabolism. They are increasingly recognized as critical modulators of a myriad of biological processes, including stress responses. Plant RBPs are heterogeneous with one or more conservative RNA motifs that constitute canonical/novel RNA binding domains (RBDs), which can bind to target RNAs to determine their regulation as per the plant requirements at given environmental conditions. Given its biological significance and possible consideration as a potential tool in genetic manipulation programs to improve key agronomic traits amidst frequent episodes of climate anomalies, studies concerning the identification and functional characterization of RBP candidate genes are steadily mounting. This paper presents a comprehensive overview of canonical and novel RBPs and their functions in major abiotic stresses including drought, heat, salt, and cold stress conditions. To some extent, we also briefly describe the basic motif structure of RBPs that would be useful in forthcoming studies. Additionally, we also collected RBP genes that were modulated by stress, but that lacked functional characterization, providing an impetus to conduct further research.


Assuntos
Proteínas de Plantas/química , Proteínas de Plantas/fisiologia , Proteínas de Ligação a RNA/química , Proteínas de Ligação a RNA/fisiologia , Estresse Fisiológico/fisiologia , Resposta ao Choque Frio/fisiologia , Secas , Resposta ao Choque Térmico/fisiologia , Domínios Proteicos , Salinidade , Estresse Salino/fisiologia
6.
Gene ; 796-797: 145806, 2021 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-34197950

RESUMO

To sustain high crop yield, a comprehensive understanding of the processes by which plants sense and acquire nutrients is of great importance. For the efficiency of crop fertilizer, it is essential to exploring the the signaling networks that coordinate the usage of nitrogen and phosphorus, the most demanding two mineral nutrients in plants. Here, we found that a protein OsCBL1 (Calcineurin B-like protein 1) is involved in the regulation of nitrogen and phosphorus signaling in rice. The nitrogen element, existing as ammonium or nitrate in the environment, affects nitrate signaling in vivo and root growth. Compared with the wild type, knockdown of OsCBL1 inhibit the growth of rice to the same extent, when nitrogen is deficient or nitrogen is present in the form of ammonium-nitrate mixture. The growth inhibition by OsCBL1-knockdown is more pronounced when nitrogen is present as ammonium. The phosphorus starvation-responsive genes is also regulated by the compound of nitrogen present in vitro and OsCBL1, while the phosphorus content is not affected. These results suggest that OsCBL1 may be involved in the response of rice to nitrogen and phosphorus nutrition in the environment, as well as the regulation of rice growth by environmental nutrition.


Assuntos
Proteínas de Ligação ao Cálcio/fisiologia , Nitratos/metabolismo , Oryza/crescimento & desenvolvimento , Fosfatos/metabolismo , Proteínas de Plantas/fisiologia , Plântula/crescimento & desenvolvimento , Proteínas de Ligação ao Cálcio/genética , Técnicas de Silenciamento de Genes , Oryza/genética , Proteínas de Plantas/genética , Plântula/genética , Transdução de Sinais
7.
Plant Mol Biol ; 106(4-5): 433-448, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34142302

RESUMO

KEY MESSAGE: Endogenous and exogenous GA3 responses to DoEXP and DoXTH depend on the DoGA20ox1, DoGA3ox1, DoGA2ox3, DoGA2ox4, DoGID1a, and DoDELLA1 to regulate yam tuber growth. Yam tuber undergoes significant alteration in morphogenesis and functions during growth, and gibberellins (GA) are considered potentially important regulators of tuber growth. However, it is little known about the regulation of GA metabolism and GA signaling components genes in tuber growth of yam. In this study, the cloning and expressions of GA3 level, GA metabolism and signaling genes, and cell wall genes in tuber growth in response to GA3 and GA biosynthesis inhibitor paclobutrazol (PP333) treatments were studied. The contents of GA3 accumulated at the tuber growth, with the highest levels in the early expansion stage. DoGA20ox1, DoGA3ox1, and four DoGA2ox genes were significantly abundant in the early expansion stage of tuber and gradually declined along with tuber growth. Three DoGID1 and three DoDELLA genes were showed different expression patterns in the early expansion stage of tuber and gradually declined along with tuber growth. Five DoEXP and three DoXTH genes expression levels were higher in the early expansion stage than in other stages. Exogenous GA3 increased endogenous GA3 levels, whereas the expression levels of DoGA20ox1, DoGA3ox1, DoGID1a, and DoDELLA1 were down-regulated in the early expansion stage of tuber by GA3 treatment, DoGA2ox3 and DoGA2ox4 were up-regulated. PP333 application exhibited opposite consequences. Thus, a mechanism of GA3 regulating yam tuber growth by DELLA-dependent pathway is established.


Assuntos
Dioscorea/crescimento & desenvolvimento , Giberelinas/metabolismo , Proteínas de Plantas/fisiologia , Tubérculos/crescimento & desenvolvimento , Clonagem Molecular , Dioscorea/genética , Dioscorea/metabolismo , Proteínas de Plantas/genética , Tubérculos/genética , Tubérculos/metabolismo
8.
J Exp Bot ; 72(13): 4822-4838, 2021 06 22.
Artigo em Inglês | MEDLINE | ID: mdl-34113976

RESUMO

Shoot branching is an important factor that influences the architecture of apple trees and cytokinin is known to promote axillary bud outgrowth. The cultivar 'Fuji', which is grown on ~75% of the apple-producing area in China, exhibits poor natural branching. The TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) family genes BRANCHED1/2 (BRC1/2) are involved in integrating diverse factors that function locally to inhibit shoot branching; however, the molecular mechanism underlying the cytokinin-mediated promotion of branching that involves the repression of BRC1/2 remains unclear. In this study, we found that apple WUSCHEL2 (MdWUS2), which interacts with the co-repressor TOPLESS-RELATED9 (MdTPR9), is activated by cytokinin and regulates branching by inhibiting the activity of MdTCP12 (a BRC2 homolog). Overexpressing MdWUS2 in Arabidopsis or Nicotiana benthamiana resulted in enhanced branching. Overexpression of MdTCP12 inhibited axillary bud outgrowth in Arabidopsis, indicating that it contributes to the regulation of branching. In addition, we found that MdWUS2 interacted with MdTCP12 in vivo and in vitro and suppressed the ability of MdTCP12 to activate the transcription of its target gene, HOMEOBOX PROTEIN 53b (MdHB53b). Our results therefore suggest that MdWUS2 is involved in the cytokinin-mediated inhibition of MdTCP12 that controls bud outgrowth, and hence provide new insights into the regulation of shoot branching by cytokinin.


Assuntos
Citocininas/fisiologia , Proteínas de Homeodomínio/fisiologia , Malus/crescimento & desenvolvimento , Proteínas de Plantas/fisiologia , Fatores de Transcrição/fisiologia , Arabidopsis/genética , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Regulação da Expressão Gênica de Plantas , Proteínas de Homeodomínio/genética , Proteínas de Plantas/genética , Brotos de Planta/crescimento & desenvolvimento , Transdução de Sinais , Fatores de Transcrição/genética
9.
Biochimie ; 188: 61-76, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34139292

RESUMO

High-scored premium wines are typically produced under moderate drought stress, suggesting that the water status of grapevine is crucial for wine quality. Aquaporins greatly influence the plant water status by facilitating water diffusion across the plasma membrane in a tightly regulated manner. They adjust the hydraulic conductance of the plasma membrane rapidly and reversibly, which is essential in specific physiological events, including adaptation to soil water scarcity. The comprehension of the sophisticated plant-water relations at the molecular level are thus important to optimize agricultural practices or to assist plant breeding programs. This review explores the recent progresses in understanding the water transport in grapevine at the cellular level through aquaporins and its regulation. Important aspects, including aquaporin structure, diversity, cellular localization, transport properties, and regulation at the cellular and whole plant level are addressed. An ecophysiological perspective about the roles of grapevine aquaporins in plant response to drought stress is also provided.


Assuntos
Aquaporinas/fisiologia , Proteínas de Plantas/fisiologia , Vitis/metabolismo , Aquaporinas/química , Transporte Biológico , Secas , Ativação do Canal Iônico , Proteínas de Plantas/química , Estruturas Vegetais/fisiologia , Estresse Fisiológico
10.
Int J Mol Sci ; 22(11)2021 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-34073055

RESUMO

Plant proline-rich proteins (PRPs) are cell wall proteins that occur in the plant kingdom and are involved in plant development and stress response. In this study, 9 PRP genes were identified from the apple genome and a comprehensive analysis of the PRP family was conducted, including gene structures, phylogenetic analysis, chromosome mapping, and so on. The expression of MdPRPs varied among tissues and in response to different types of stresses. MdPRP4 and MdPRP7 were induced by five detected stress treatments, including heat, drought, abscisic acid, cold, and salt; the expression patterns of the others varied under different types of stress. Subcellular localization showed that MdPRPs mainly functioned in the cytoplasm, except for MdPRP1 and MdPRP5, which also functioned in the nucleus. When MdPRP6 was overexpressed in tobacco, the transgenic plants showed higher tolerance to high temperature (48 °C) compared with wild-type (WT) plants. The transgenic plants showed milder wilting, a lower accumulation of electrolyte leakage, MDA and ROS, and a higher level of chlorophyll and SOD and POD activity, indicating that MdPRP6 may be an important gene in apples for heat stress tolerance. Overall, this study suggested that MdPRPs are critically important for the ability of apple responses to stresses.


Assuntos
Malus/genética , Proteínas de Plantas , Domínios Proteicos Ricos em Prolina , Estresse Fisiológico , Temperatura Alta , Proteínas de Plantas/genética , Proteínas de Plantas/fisiologia , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/metabolismo , Tabaco/genética , Tabaco/metabolismo
11.
BMC Plant Biol ; 21(1): 286, 2021 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-34157966

RESUMO

BACKGROUND: Brassica napus is an essential crop for oil and livestock feed. Eventually, this crop's economic interest is at the most risk due to anthropogenic climate change. DELLA proteins constitute a significant repressor of plant growth to facilitate survival under constant stress conditions. DELLA proteins lack DNA binding domain but can interact with various transcription factors or transcription regulators of different hormonal families. Significant progress has been made on Arabidopsis and cereal plants. However, no comprehensive study regarding DELLA proteins has been delineated in rapeseed. RESULTS: In our study, we have identified 10 BnaDELLA genes. All of the BnaDELLA genes are closely related to five AtDELLA genes, suggesting a relative function and structure. Gene duplication and synteny relationship among Brassica. napus, Arabidopsis. thaliana, Brassica rapa, Brassica oleracea, and Brassica nigra genomes were also predicted to provide valuable insights into the BnaDELLA gene family evolutionary characteristics. Chromosomal mapping revealed the uneven distribution of BnaDELLA genes on eight chromosomes, and site-specific selection assessment proposes BnaDELLA genes purifying selection. The motifs composition in all BnaDELLA genes is inconsistent; however, every BnaDELLA gene contains 12 highly conserved motifs, encoding DELLA and GRAS domains. The two known miRNAs (bna-miR6029 and bna-miR603) targets BnaC07RGA and BnaA09GAI, were also predicted. Furthermore, quantitative real-time PCR (qRT-PCR) analysis has exhibited the BnaDELLA genes diverse expression patterns in the root, mature-silique, leaf, flower, flower-bud, stem, shoot-apex, and seed. Additionally, cis-acting element prediction shows that all BnaDELLA genes contain light, stress, and hormone-responsive elements on their promoters. The gene ontology (GO) enrichment report indicated that the BnaDELLA gene family might regulate stress responses. Combine with transcriptomic data used in this study, we detected the distinct expression patterns of BnaDELLA genes under biotic and abiotic stresses. CONCLUSION: In this study, we investigate evolution feature, genomic structure, miRNAs targets, and expression pattern of the BnaDELLA gene family in B. napus, which enrich our understanding of BnaDELLA genes in B. napus and suggests modulating individual BnaDELLA expression is a promising way to intensify rapeseed stress tolerance and harvest index.


Assuntos
Brassica napus/genética , Genes de Plantas/genética , Brassica napus/fisiologia , Mapeamento Cromossômico , Cromossomos de Plantas/genética , Evolução Molecular , Genes de Plantas/fisiologia , Estudo de Associação Genômica Ampla , MicroRNAs/genética , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/fisiologia , RNA de Plantas/genética , Alinhamento de Sequência , Estresse Fisiológico , Transcriptoma
12.
BMC Plant Biol ; 21(1): 284, 2021 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-34157974

RESUMO

BACKGROUND: Identifying genes involved in salt tolerance in the recretohalophyte Limonium bicolor could facilitate the breeding of crops with enhanced salt tolerance. Here we cloned the previously uncharacterized gene LbHLH and explored its role in salt tolerance. RESULTS: The 2,067-bp open reading frame of LbHLH encodes a 688-amino-acid protein with a typical helix-loop-helix (HLH) domain. In situ hybridization showed that LbHLH is expressed in salt glands of L. bicolor. LbHLH localizes to the nucleus, and LbHLH is highly expressed during salt gland development and in response to NaCl treatment. To further explore its function, we heterologously expressed LbHLH in Arabidopsis thaliana under the 35S promoter. The overexpression lines showed significantly increased trichome number and reduced root hair number. LbHLH might interact with GLABRA1 to influence trichome and root hair development, as revealed by yeast two-hybrid analysis. The transgenic lines showed higher germination percentages and longer roots than the wild type under NaCl treatment. Analysis of seedlings grown on medium containing sorbitol with the same osmotic pressure as 100 mM NaCl demonstrated that overexpressing LbHLH enhanced osmotic resistance. CONCLUSION: These results indicate that LbHLH enhances salt tolerance by reducing root hair development and enhancing osmotic resistance under NaCl stress.


Assuntos
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Genes de Plantas/genética , Proteínas de Plantas/genética , Raízes de Plantas/crescimento & desenvolvimento , Plumbaginaceae/genética , Plantas Tolerantes a Sal/genética , Fatores de Transcrição Hélice-Alça-Hélice Básicos/fisiologia , Clonagem Molecular , Genes de Plantas/fisiologia , Hibridização In Situ , Pressão Osmótica , Proteínas de Plantas/fisiologia , Plumbaginaceae/metabolismo , Plumbaginaceae/fisiologia , Reação em Cadeia da Polimerase , Estresse Salino , Tolerância ao Sal/genética , Plantas Tolerantes a Sal/metabolismo , Plantas Tolerantes a Sal/fisiologia , Técnicas do Sistema de Duplo-Híbrido
13.
BMC Plant Biol ; 21(1): 291, 2021 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-34167462

RESUMO

Brassinosteroids (BRs) play important roles in plant growth and development. Although BR receptors have been intensively studied in Arabidopsis, those in foxtail millet remain largely unknown. Here, we show that the BR signaling function of BRASSINOSTEROID INSENSITIVE 1 (BRI1) is conserved between Arabidopsis and foxtail millet, a new model species for C4 and Panicoideae grasses. We identified four putative BR receptor genes in the foxtail millet genome: SiBRI1, SiBRI1-LIKE RECEPTOR KINASE 1 (SiBRL1), SiBRL2 and SiBRL3. Phylogenetic analysis was used to classify the BR receptors in dicots and monocots into three branches. Analysis of their expression patterns by quantitative real-time PCR (qRT-PCR) showed that these receptors were ubiquitously expressed in leaves, stems, dark-grown seedlings, roots and non-flowering spikelets. GFP fusion experiments verified that SiBRI1 localized to the cell membrane. We also explored the SiBRI1 function in Arabidopsis through complementation experiments. Ectopic overexpression of SiBRI1 in an Arabidopsis BR receptor loss-of-function mutant, bri1-116, mostly reversed the developmental defects of the mutant. When SiBRI1 was overexpressed in foxtail millet, the plants showed a drooping leaf phenotype and root development inhibition, lateral root initiation inhibition, and the expression of BR synthesis genes was inhibited. We further identified BRI1-interacting proteins by immunoprecipitation (IP)-mass spectrometry (MS). Our results not only demonstrate that SiBRI1 plays a conserved role in BR signaling in foxtail millet but also provide insight into the molecular mechanism of SiBRI1.


Assuntos
Brassinosteroides/metabolismo , Genes de Plantas/genética , Proteínas de Plantas/genética , Receptores de Superfície Celular/genética , Setaria (Planta)/genética , Arabidopsis/genética , Arabidopsis/fisiologia , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/fisiologia , Evolução Molecular , Filogenia , Proteínas de Plantas/metabolismo , Proteínas de Plantas/fisiologia , Raízes de Plantas/crescimento & desenvolvimento , Proteínas Quinases/genética , Proteínas Quinases/fisiologia , Receptores de Superfície Celular/metabolismo , Receptores de Superfície Celular/fisiologia , Setaria (Planta)/metabolismo
14.
BMC Plant Biol ; 21(1): 292, 2021 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-34167472

RESUMO

BACKGROUND: Parthenocarpy results in traits attractive to both consumers and breeders, and it overcomes the obstacle of self-incompatibility in the fruit set of horticultural crops, including pear (Pyrus bretshneider). However, there is limited knowledge regarding the genetic and molecular mechanisms that regulate parthenogenesis. RESULTS: Here, in a transcriptional comparison between pollination-dependent fruit and GA4-induced parthenocarpy, PbCYP78A6 was identified and proposed as a candidate gene involved in parthenocarpy. PbCYP78A6 is similar to Arabidopsis thaliana CYP78A6 and highly expressed in pear hypanthia. The increased PbCYP78A6 expression, as assessed by RT-qPCR, was induced by pollination and GA4 exposure. The ectopic overexpression of PbCYP78A6 contributed to parthenocarpic fruit production in tomato. The PbCYP78A6 expression coincided with fertilized and parthenocarpic fruitlets development and the expression of fruit development-related genes as assessed by cytological observations and RT-qPCR, respectively. PbCYP78A6 RNA interference and overexpression in pear calli revealed that the gene is an upstream regulator of specific fruit development-related genes in pear. CONCLUSIONS: Our findings indicate that PbCYP78A6 plays a critical role in fruit formation and provide insights into controlling parthenocarpy.


Assuntos
Ciclo Celular , Sistema Enzimático do Citocromo P-450/genética , Genes de Plantas/genética , Partenogênese , Proteínas de Plantas/genética , Pyrus/metabolismo , Ciclo Celular/genética , Ciclo Celular/fisiologia , Sistema Enzimático do Citocromo P-450/fisiologia , Perfilação da Expressão Gênica , Genes de Plantas/fisiologia , Partenogênese/genética , Partenogênese/fisiologia , Filogenia , Proteínas de Plantas/fisiologia , Polinização , Pyrus/genética , Pyrus/crescimento & desenvolvimento , Pyrus/fisiologia
15.
BMC Plant Biol ; 21(1): 295, 2021 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-34174836

RESUMO

BACKGROUND: Drought is a common phenomenon worldwide. It is also one of the main abiotic factors that affect the growth and quality of strawberry. The dehydration-responsive element binding protein (DREB) members that belong to the APETALA2/ethylene-responsive element binding protein (AP2/EREBP) superfamily are unique transcription factors in plants that play important roles in the abiotic stress response. RESULTS: Here, a total of 119 AP2/EREBP genes were identified in Fragaria vesca, and the AP2/EREBP superfamily was divided into AP2, RAV, ERF, DREB, and soloist subfamilies, containing 18, 7, 61, 32, and one member(s), respectively. The DREB subfamily was further divided into six subgroups (A-1 to A-6) based on phylogenetic analysis. Gene structure, conserved motifs, chromosomal location, and synteny analysis were conducted to comprehensively investigate the characteristics of FvDREBs. Furthermore, transcriptome analysis revealed distinctive expression patterns among the FvDREB genes in strawberry plants exposed to drought stress. The expression of FvDREB6 of the A-2 subgroup was down-regulated in old leaves and up-regulated in young leaves in response to drought. Furthermore, qRT-PCR analysis found that FvDREB8 from the A-2 subgroup had the highest expression level under drought stress. Together, analyses with the expression pattern, phylogenetic relationship, motif, and promoter suggest that FvDREB18 may play a critical role in the regulation of FvDREB1 and FvDREB2 expression. CONCLUSIONS: Our findings provide new insights into the characteristics and potential functions of FvDREBs. These FvDREB genes should be further studied as they appear to be excellent candidates for drought tolerance improvement of strawberry.


Assuntos
Fragaria/genética , Regulação da Expressão Gênica de Plantas/genética , Genes de Plantas/genética , Proteínas de Plantas/genética , Fatores de Transcrição/genética , Transcriptoma , Sequência Conservada , Desidratação , Fragaria/metabolismo , Fragaria/fisiologia , Regulação da Expressão Gênica de Plantas/fisiologia , Genes de Plantas/fisiologia , Estudo de Associação Genômica Ampla , Proteínas de Plantas/fisiologia , Reação em Cadeia da Polimerase em Tempo Real , Fatores de Transcrição/fisiologia
16.
BMC Plant Biol ; 21(1): 301, 2021 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-34187365

RESUMO

BACKGROUND: Mustard (Brassica juncea) is an important economic vegetable, and some cultivars have purple leaves and accumulate more anthocyanins than the green. The genetic and evolution of purple trait in mustard has not been well studied. RESULT: In this study, free-hand sections and metabolomics showed that the purple leaves of mustard accumulated more anthocyanins than green ones. The gene controlling purple leaves in mustard, Mustard Purple Leaves (MPL), was genetically mapped and a MYB113-like homolog was identified as the candidate gene. We identified three alleles of the MYB113-like gene, BjMYB113a from a purple cultivar, BjMYB113b and BjMYB113c from green cultivars. A total of 45 single nucleotide polymorphisms (SNPs) and 8 InDels were found between the promoter sequences of the purple allele BjMYB113a and the green allele BjMYB113b. On the other hand, the only sequence variation between the purple allele BjMYB113a and the green allele BjMYB113c is an insertion of 1,033-bp fragment in the 3'region of BjMYB113c. Transgenic assay and promoter activity studies showed that the polymorphism in the promoter region was responsible for the up-regulation of the purple allele BjMYB113a and high accumulation of anthocyanin in the purple cultivar. The up-regulation of BjMYB113a increased the expression of genes in the anthocyanin biosynthesis pathway including BjCHS, BjF3H, BjF3'H, BjDFR, BjANS and BjUGFT, and consequently led to high accumulation of anthocyanin. However, the up-regulation of BjMYB113 was compromised by the insertion of 1,033-bp in 3'region of the allele BjMYB113c. CONCLUSIONS: Our results contribute to a better understanding of the genetics and evolution of the BjMYB113 gene controlling purple leaves and provide useful information for further breeding programs of mustard.


Assuntos
Genes de Plantas/genética , Mutação com Perda de Função/genética , Mostardeira/genética , Folhas de Planta/anatomia & histologia , Proteínas de Plantas/genética , Fatores de Transcrição/genética , Alelos , Antocianinas/metabolismo , Arabidopsis , Clonagem Molecular , Cor , Genes de Plantas/fisiologia , Mostardeira/anatomia & histologia , Mostardeira/metabolismo , Folhas de Planta/metabolismo , Proteínas de Plantas/fisiologia , Plantas Geneticamente Modificadas , Fatores de Transcrição/fisiologia
17.
Int J Mol Sci ; 22(11)2021 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-34073690

RESUMO

E3 ubiquitin ligases, the most important part of the ubiquitination process, participate in various processes of plant immune response. RBR E3 ligase is one of the E3 family members, but its functions in plant immunity are still little known. NtRNF217 is a RBR E3 ligase in tobacco based on the sequence analysis. To assess roles of NtRNF217 in tobacco responding to Ralstonia solanacearum, overexpression experiments in Nicotiana tabacum (Yunyan 87, a susceptible cultivar) were performed. The results illuminated that NtRNF217-overexpressed tobacco significantly reduced multiplication of R. solanacearum and inhibited the development of disease symptoms compared with wild-type plants. The accumulation of H2O2 and O2- in NtRNF217-OE plants was significantly higher than that in WT-Yunyan87 plants after pathogen inoculation. The activities of CAT and SOD also increased rapidly in a short time after R. solanacearum inoculation in NtRNF217-OE plants. What is more, overexpression of NtRNF217 enhanced the transcript levels of defense-related marker genes, such as NtEFE26, NtACC Oxidase, NtHIN1, NtHSR201, and NtSOD1 in NtRNF217-OE plants after R. solanacearum inoculation. The results suggested that NtRNF217 played an important role in regulating the expression of defense-related genes and the antioxidant enzymes, which resulted in resistance to R. solanacearum infection.


Assuntos
Resistência à Doença , Doenças das Plantas , Ralstonia solanacearum , Tabaco/metabolismo , Ubiquitina-Proteína Ligases/fisiologia , Infecções Bacterianas/metabolismo , Regulação da Expressão Gênica de Plantas , Peróxido de Hidrogênio , Filogenia , Proteínas de Plantas/fisiologia , Espécies Reativas de Oxigênio , Tabaco/genética , Tabaco/fisiologia , Ubiquitina-Proteína Ligases/genética , Ubiquitina-Proteína Ligases/metabolismo
18.
BMC Plant Biol ; 21(1): 253, 2021 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-34082706

RESUMO

BACKGROUND: Root hydraulic conductance is primarily determined by the conductance of living tissues to radial water flow. Plasma membrane intrinsic proteins (PIPs) in root cortical cells are important for plants to take up water and are believed to be directly involved in cell growth. RESULTS: In this study, we found that constitutive overexpression of the poplar root-specific gene PtoPIP1;1 in Arabidopsis accelerated bolting and flowering. At the early stage of the developmental process, PtoPIP1;1 OE Arabidopsis exhibited faster cell growth in both leaves and roots. The turgor pressure of plants was correspondingly increased in PtoPIP1;1 OE Arabidopsis, and the water status was changed. At the same time, the expression levels of flowering-related genes (CRY1, CRY2 and FCA) and hub genes in the regulatory networks underlying floral timing (FT and SOC1) were significantly upregulated in OE plants, while the floral repressor FLC gene was significantly downregulated. CONCLUSIONS: Taken together, the results of our study indicate that constitutive overexpression of PtoPIP1;1 in Arabidopsis accelerates bolting and flowering through faster cell growth in both the leaf and root at an early stage of the developmental process. The autonomous pathway of flowering regulation may be executed by monitoring developmental age. The increase in turgor and changes in water status with PtoPIP1;1 overexpression play a role in promoting cell growth.


Assuntos
Aquaporinas/fisiologia , Arabidopsis/crescimento & desenvolvimento , Arabidopsis/genética , Proteínas de Plantas/metabolismo , Populus/metabolismo , Flores/crescimento & desenvolvimento , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Regulação da Expressão Gênica de Plantas/fisiologia , Folhas de Planta/citologia , Folhas de Planta/crescimento & desenvolvimento , Proteínas de Plantas/genética , Proteínas de Plantas/fisiologia , Raízes de Plantas/citologia , Raízes de Plantas/crescimento & desenvolvimento , Transpiração Vegetal , Plantas Geneticamente Modificadas , Populus/genética
19.
Ann Bot ; 128(2): 217-230, 2021 07 30.
Artigo em Inglês | MEDLINE | ID: mdl-33959756

RESUMO

BACKGROUND AND AIMS: The ovule is a synapomorphy of all seed plants (gymnosperms and angiosperms); however, there are some striking differences in ovules among the major seed plant lineages, such as the number of integuments or the orientation of the ovule. The genetics involved in ovule development have been well studied in the model species Arabidopsis thaliana, which has two integuments and anatropous orientation. This study is approached from what is known in arabidopsis, focusing on the expression patterns of homologues of four genes known to be key for the proper development of the integuments in arabidopsis: AINTEGUMENTA (ANT), BELL1, (BEL1), KANADIs (KANs) and UNICORN (UCN). METHODS: We used histology to describe the morphoanatomical development from ovules to seeds in Gnetum gnemon. We carried out spatiotemporal expression analyses in G. gnemon, a gymnosperm, which has a unique ovule morphology with an integument covering the nucellus, two additional envelopes where the outermost becomes fleshy as the seed matures, and an orthotropous orientation. KEY RESULTS: Our anatomical and developmental descriptions provide a framework for expression analyses in the ovule of G. gnemon. Our expression results show that although ANT, KAN and UCN homologues are expressed in the inner integument, their spatiotemporal patterns differ from those found in angiosperms. Furthermore, all homologues studied here are expressed in the nucellus, revealing major differences in seed plants. Finally, no expression of the studied homologues was detected in the outer envelopes. CONCLUSIONS: Altogether, these analyses provide significant comparative data that allows us to better understand the functional evolution of these gene lineages, providing a compelling framework for evolutionary and developmental studies of seeds. Our findings suggest that these genes were most likely recruited from the sporangium development network and became restricted to the integuments of angiosperm ovules.


Assuntos
Gnetum , Óvulo Vegetal , Proteínas de Plantas/genética , Regulação da Expressão Gênica de Plantas , Redes Reguladoras de Genes , Gnetum/genética , Gnetum/crescimento & desenvolvimento , Óvulo Vegetal/genética , Óvulo Vegetal/crescimento & desenvolvimento , Proteínas de Plantas/fisiologia , Fatores de Transcrição/genética , Fatores de Transcrição/fisiologia
20.
J Plant Physiol ; 262: 153439, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34023806

RESUMO

Late stages of floret development, such as booting, heading, and anthesis stages, are important steps for determining grain setting and for filling in wheat. Herein, we report the molecular function of Triticum aestivum ELF7 encoding RNA polymerase II-associated factor 1 (PAF1), which may act as a negative regulator in floret development and anthesis stages. Among the six TaELF7-like genes isolated from wheat, TaELF7 like1-A and TaELF7 like2-B showed contrasting expression levels during the late stage of floret development stages, with observation of decreased expression level of TaELF7 like1-A compared to that of TaELF7 like2-B. The full-length TaELF7 like1-A has a 1038-bp open reading frame that contains a proline-rich domain in the N-terminal region and a nuclear localization signal domain in the C-terminal region. TaELF7 like1-A was found to be localized in the nucleus in both tobacco and wheat. Direct interaction of TaELF7 with the RING-type E3 ligase TaHUB2 was confirmed using a yeast two-hybrid system, an in vitro pull-down assay, and a bimolecular fluorescence complementation assay. The flowering time was delayed in TaELF7-overexpressing plants compared to that in the control plants. Expression levels of few floral repressor genes were markedly increased in TaELF7-overexpressing Arabidopsis plants.


Assuntos
Arabidopsis/crescimento & desenvolvimento , Flores/crescimento & desenvolvimento , Proteínas de Plantas/fisiologia , Fatores de Transcrição/fisiologia , Triticum/metabolismo , Arabidopsis/genética , Arabidopsis/metabolismo , Regulação da Expressão Gênica de Plantas , Genes de Plantas/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Análise de Sequência de DNA , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Transcriptoma , Técnicas do Sistema de Duplo-Híbrido
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...