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1.
Einstein (Sao Paulo) ; 18: eRC5063, 2020.
Artigo em Inglês, Português | MEDLINE | ID: mdl-31553357

RESUMO

A 65-year-old male with a history of urinary tract trauma requiring cystotomy and chronic bladder catheterization, presenting with chronic and uninvestigated changes in the color of the urine bag system, with no urine color change, and positive urine culture for Proteus mirabilis . These characteristics refer to the purple urine bag syndrome, a not weel-known condition, with a benign course in most cases, and associated with urinary tract infection in patients with chronic bladder catheterization. Although it is characterized by marked changes, it is underdiagnosed by healthcare professionals.


Assuntos
Infecções Relacionadas a Cateter/microbiologia , Coletores de Urina/efeitos adversos , Infecções Urinárias/microbiologia , Idoso , Infecções Relacionadas a Cateter/patologia , Humanos , Masculino , Proteus mirabilis/isolamento & purificação , Fatores de Risco , Síndrome , Infecções Urinárias/patologia , Urina/microbiologia
2.
BMC Res Notes ; 12(1): 244, 2019 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-31036061

RESUMO

OBJECTIVES: Diabetic foot ulcers (DFUs) often lead to hospital admissions, amputations and deaths; however, there is no up-to-date information on microbial isolates from DFUs and no mention of utilization of molecular techniques in Sub-Saharan Africa. We conducted a cross-sectional study among 83 adult patients at a tertiary hospital in Kenya over 12 months. The study aimed to isolate, identify bacteria, their antibiotic susceptibility patterns in active DFUs, and to compare standard microbiological methods versus a real-time PCR commercial kit in the detection of Staphylococcus aureus DNA and methicillin-resistant S. aureus (MRSA) DNA. RESULTS: Eighty swabs (94%) were culture-positive; 29% were Gram-positive and 65% were Gram-negative. The main organisms isolated were S. aureus (16%), Escherichia coli (15%), Proteus mirabilis (11%), Klebsiella pneumoniae (7%) and Pseudomonas aeruginosa (7%). The bacterial isolates showed resistance to commonly used antibiotics such as ampicillin, amoxicillin, cefepime, ceftazidime, cefuroxime, clindamycin, erythromycin, piperacillin-tazobactam, tetracycline and trimethoprim-sulphamethoxazole (TMPSMX). Thirty-one percent of the S. aureus isolated and 40% of the Gram-negatives were multi-drug resistant organisms (MDROs). There was a high prevalence of nosocomial bacteria. MRSA were not identified using culture methods but were identified using PCR. PCR was more sensitive but less specific than culture-based methods to identify S. aureus.


Assuntos
Antibacterianos/uso terapêutico , Infecções Bacterianas/diagnóstico , Pé Diabético/diagnóstico , Farmacorresistência Bacteriana Múltipla , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Infecções Bacterianas/tratamento farmacológico , Infecções Bacterianas/epidemiologia , Infecções Bacterianas/microbiologia , Técnicas de Tipagem Bacteriana , Cefalosporinas/uso terapêutico , Clindamicina/uso terapêutico , Estudos Transversais , Pé Diabético/tratamento farmacológico , Pé Diabético/epidemiologia , Pé Diabético/microbiologia , Escherichia coli/classificação , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Escherichia coli/isolamento & purificação , Humanos , Quênia/epidemiologia , Klebsiella pneumoniae/classificação , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/genética , Klebsiella pneumoniae/isolamento & purificação , Macrolídeos/uso terapêutico , Staphylococcus aureus Resistente à Meticilina/classificação , Staphylococcus aureus Resistente à Meticilina/genética , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Testes de Sensibilidade Microbiana , Penicilinas/uso terapêutico , Proteus mirabilis/classificação , Proteus mirabilis/efeitos dos fármacos , Proteus mirabilis/genética , Proteus mirabilis/isolamento & purificação , Pseudomonas aeruginosa/classificação , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/genética , Pseudomonas aeruginosa/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real , Staphylococcus aureus/classificação , Staphylococcus aureus/genética , Staphylococcus aureus/isolamento & purificação , Sulfanilamidas/uso terapêutico
3.
Artigo em Inglês | MEDLINE | ID: mdl-30912480

RESUMO

Urinary tract infections (UTIs) have been frequently reported from different parts of the world. The current knowledge on distribution of causative agents of urinary infections and antibiotics susceptibility pattern is essentially required. In the present study, total 351 uropathogenic bacteria were isolated; among them most prevalent were Escherichia coli (75%), followed by Pseudomonas aeruginosa (8%), Proteus mirabilis (6%), Klebsiella pneumoniae (4%), Staphylococcus aureus (4%) and Enterococcus faecalis (3%). Most isolates of uropathogenic bacteria showed resistance to amoxicillin and trimethoprim, followed by chloramphenicol and kanamycin. Biosynthesis of sulfur nanoparticles (SNPs) was performed by co-precipitation method using sodium thiosulfate in presence of Catharanthus roseus leaf extract. The characterization data showed that SNPs were polydispersed, spherical in shape with size range of 20-86 nm and having negative zeta potential of -9.24 mV. The potential antibacterial activity was observed for SNPs alone and in combination with antibiotics particularly amoxicillin and trimethoprim against majority of the uropathogens. The synergistic effect yielded increase in fold area with high activity index against tested uropathogens. Based on overall results, it can be recommended to use SNPs for the management of UTI alone and also in combination with antibiotics.


Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos , Nanopartículas/química , Enxofre/farmacologia , Infecções Urinárias/microbiologia , Antibacterianos/química , Sinergismo Farmacológico , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Humanos , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/isolamento & purificação , Testes de Sensibilidade Microbiana , Proteus mirabilis/efeitos dos fármacos , Proteus mirabilis/isolamento & purificação , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/isolamento & purificação , Enxofre/química
4.
Pak J Biol Sci ; 22(1): 45-50, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30796768

RESUMO

BACKGROUND AND OBJECTIVE: Efforts at organic mercury detoxification can be carried out using resistant bacteria that can live in an environment contaminated with the compound. This study aimed at isolating and identifying resistant bacteria from mercury-contaminated environments and analysing their ability to detoxify organic mercury. MATERIALS AND METHODS: Soil samples were obtained from 3 gold processing locations that make use of mercury in Tanoyan Village, Bolaang Mongondow district, North Sulawesi province. The identification was carried out on the mercury-resistant bacteria through morphological and molecular tests. Bacteria which were highly resistant to mercury were examined for their ability to detoxify phenyl mercury (organic mercury). RESULTS: The study showed that 8 mercury-resistant bacterial colonies could be isolated from the three soil samples. The bacteria were able to grow in LB broth containing 10 mg L-1 of phenyl mercury. Four isolates (AA, BB, CC and DD) were even able to grow in 40 mg L-1 of phenyl mercury. According to the identification tests, those bacteria were Pseudomonas sp. (AA, DD), Pseudomonas aeruginosa (BB) and Proteus mirabilis (CC). Testing of organic mercury against isolates of bacteria which are highly resistant to it in order to determine their detoxification capacity revealed that all four isolates could reduce levels of the compound in media, based on the results, starting from the highest was Pseudomonas sp. 74.99%, then Pseudomonas aeruginosa 60.23% and Proteus mirabilis 47.59% after 24 h of incubation. CONCLUSION: The study suggested that there are four bacteria that have potentials to remediate organic mercury contamination sites.


Assuntos
Bactérias/isolamento & purificação , Mercúrio/efeitos adversos , Biodegradação Ambiental , Indonésia , Proteus mirabilis/isolamento & purificação , Pseudomonas/isolamento & purificação
5.
Can J Microbiol ; 65(5): 377-386, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30657697

RESUMO

This study aimed to harness the benefits of sulfur-oxidizing beneficial bacteria from buffalo dung to improve crop yields of Foeniculum vulgare. A total of 61 bacterial isolates were screened from buffalo dung, of which 40 isolates exhibited plant-growth-promoting attributes, such as phosphate solubilization, indole-3-acetic acid production, and hydrogen cyanide production. Of these 40, four bacterial isolates, viz., BUFF12, BUFF14, BUFF23, and BUFF38, were the most potent, having plant-growth-promoting and sulfur-oxidizing properties. These four isolates produced phytase by solubilizing calcium phytate and sodium phytate. They solubilized potassium besides oxidizing the sulfur, causing an increase in soil fertility and crop production. All four isolates were nonpathogenic in nature, as demonstrated by a negative haemolysis test. According to the 16S rRNA gene sequence, the isolate BUFF14 was identified as Proteus mirabilis. Proteus mirabilis BUFF14 maximized seed germination with enhanced vegetative and reproductive parameters during pot and field trial studies, compared with the other isolates.


Assuntos
Bactérias/metabolismo , Búfalos , Fezes/microbiologia , Foeniculum/crescimento & desenvolvimento , Microbiologia do Solo , Animais , Bactérias/genética , Ácidos Indolacéticos/metabolismo , Fosfatos , Desenvolvimento Vegetal , Proteus mirabilis/isolamento & purificação , Proteus mirabilis/metabolismo , RNA Ribossômico 16S/genética , Solo , Enxofre/metabolismo
6.
Nat Microbiol ; 4(3): 492-503, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30643240

RESUMO

Primary sclerosing cholangitis (PSC) is a chronic inflammatory liver disease and its frequent complication with ulcerative colitis highlights the pathogenic role of epithelial barrier dysfunction. Intestinal barrier dysfunction has been implicated in the pathogenesis of PSC, yet its underlying mechanism remains unknown. Here, we identify Klebsiella pneumonia in the microbiota of patients with PSC and demonstrate that K. pneumoniae disrupts the epithelial barrier to initiate bacterial translocation and liver inflammatory responses. Gnotobiotic mice inoculated with PSC-derived microbiota exhibited T helper 17 (TH17) cell responses in the liver and increased susceptibility to hepatobiliary injuries. Bacterial culture of mesenteric lymph nodes in these mice isolated K. pneumoniae, Proteus mirabilis and Enterococcus gallinarum, which were prevalently detected in patients with PSC. A bacterial-organoid co-culture system visualized the epithelial-damaging effect of PSC-derived K. pneumoniae that was associated with bacterial translocation and susceptibility to TH17-mediated hepatobiliary injuries. We also show that antibiotic treatment ameliorated the TH17 immune response induced by PSC-derived microbiota. These results highlight the role of pathobionts in intestinal barrier dysfunction and liver inflammation, providing insights into therapeutic strategies for PSC.


Assuntos
Colangite Esclerosante/imunologia , Microbioma Gastrointestinal , Intestinos/patologia , Klebsiella pneumoniae/patogenicidade , Fígado/imunologia , Células Th17/imunologia , Adulto , Idoso , Animais , Translocação Bacteriana , Colangite Esclerosante/microbiologia , Colite Ulcerativa/complicações , Enterococcus/isolamento & purificação , Células Epiteliais/microbiologia , Células Epiteliais/patologia , Feminino , Vida Livre de Germes , Humanos , Intestinos/imunologia , Klebsiella pneumoniae/isolamento & purificação , Fígado/patologia , Linfonodos/microbiologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Pessoa de Meia-Idade , Organoides/microbiologia , Proteus mirabilis/isolamento & purificação
8.
BMJ Case Rep ; 11(1)2018 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-30567169

RESUMO

Mycotic (infected) aneurysm involving the thoracic aorta is an exceedingly rare and life-threatening condition that is associated with high morbidity and mortality. We report an unusual source of Proteus mirabilis bacteraemia thought to be due to an infected aneurysm in the thoracic aortic arch in an elderly woman. Source of gram-negative bacteraemia is usually isolated to an intra-abdominal or a pelvic source. Proteus bacteraemia from an intrathoracic pathology is very uncommon, and in this case led to a delay in diagnosis. Although an infected aneurysm is a rare source of gram-negative bacteraemia, it must always be considered when common causes of bacteraemia have been ruled out especially in patients with vascular risk factors.


Assuntos
Aneurisma Infectado/diagnóstico , Aneurisma da Aorta Torácica/diagnóstico , Bacteriemia/diagnóstico , Infecções por Proteus/diagnóstico , Proteus mirabilis/isolamento & purificação , Idoso de 80 Anos ou mais , Aneurisma Infectado/diagnóstico por imagem , Aneurisma Infectado/terapia , Antibacterianos/administração & dosagem , Antibacterianos/uso terapêutico , Aneurisma da Aorta Torácica/diagnóstico por imagem , Aneurisma da Aorta Torácica/terapia , Bacteriemia/tratamento farmacológico , Implante de Prótese Vascular , Ciprofloxacino/administração & dosagem , Ciprofloxacino/uso terapêutico , Angiografia por Tomografia Computadorizada , Diagnóstico Diferencial , Feminino , Humanos , Infecções por Proteus/tratamento farmacológico , Stents
9.
Medicine (Baltimore) ; 97(33): e11796, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30113467

RESUMO

Urinary tract infections due to the presence of a urinary catheter represent a real problem for patients who have to carry such an invasive device for a long time.Our aim was to identify the susceptibility of extended spectrum beta lactamases (ESBL) versus non-ESBL bacteria to antibiotics in urinary tract infections in patients who are chronic carriers of urinary catheters.The retrospective study included a period of 5 years, a total of 405 patients who are chronic carriers of urinary catheters, admitted to rehabilitation and palliative care units.Escherichia coli (E coli) was isolated in 41.2% of patients, Klebsiella pneumoniae (K pneumoniae) in 24.7%, and Proteus mirabilis (P mirabilis) in 15.3%. E coli microbial resistance rates ranged from a minimum of 7.5% (nitrofurantoin) to a maximum of 77.1% (ampicillin). In the case of K pneumoniae, microbial resistance ranged from 34.2% (netilmicin) to 73.2% (ceftriaxone). Resistance rates of P mirabilis ranged from 11.1% (cefepim) to 89.5% (ampicillin). Positivity of ESBL bacteria was identified in 47.4% of patients. Resistance rates of ESBL-positive E coli ranged from 50.0% (ceftriaxone) to 88.1% (cefepime), and ESBL-negative E coli rates ranged from 3.4% (cefepime) to 64.4% (amikacin). Resistance rates of ESBL-positive K pneumoniae ranged between 39.1% (netilmicin) and 85.1% (ceftriaxone), and ESBL-negative K pneumoniae between 7.1% (cefepime) and 53.3% (amikacin). In cases of ESBL-positive P mirabilis, rates ranged from 13.3% (cefepime) to 90.3% (ceftriaxone), whereas in cases of ESBL-negative P mirabilis, rates ranged between 8.3% (cefepime) and 80.0% (trimetroprim).Bacteriuria and asymptomatic catheter infection in chronic carriers is an important public health concern due to the frequent presence of multidrug-resistant bacteria. Our study highlights the need to develop control programs of catheter infections to minimize the risk of infections associated with these medical devices, and also the need for treatment of the infection rather than catheter colonization or contamination.


Assuntos
Infecções Assintomáticas/epidemiologia , Bactérias/efeitos dos fármacos , Bacteriúria/microbiologia , Cateteres de Demora/microbiologia , Infecções por Enterobacteriaceae/epidemiologia , Infecções por Escherichia coli/diagnóstico , Cateteres Urinários/microbiologia , Infecções Urinárias/microbiologia , beta-Lactamases/biossíntese , Adulto , Idoso , Antibacterianos/uso terapêutico , Bactérias/patogenicidade , Bacteriúria/diagnóstico , Bacteriúria/epidemiologia , Bacteriúria/prevenção & controle , Cateteres de Demora/efeitos adversos , Farmacorresistência Bacteriana , Resistência a Múltiplos Medicamentos , Infecções por Enterobacteriaceae/tratamento farmacológico , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/tratamento farmacológico , Feminino , Humanos , Incidência , Klebsiella pneumoniae/isolamento & purificação , Masculino , Pessoa de Meia-Idade , Proteus mirabilis/isolamento & purificação , Estudos Retrospectivos , Cateteres Urinários/efeitos adversos , Infecções Urinárias/diagnóstico , Infecções Urinárias/epidemiologia , Infecções Urinárias/prevenção & controle , beta-Lactamases/efeitos dos fármacos
10.
Int J Biol Macromol ; 118(Pt A): 1131-1135, 2018 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-30001599

RESUMO

The current serological classification scheme of the medically important bacteria from the genus Proteus consists of 80 O serogroups, the last four of which (O77-O80) were created from clinical strains from Lódz, Poland. There are more serologically unique strains isolated from patient that do not fit into the existing scheme, such as Proteus mirabilis strain Sm 99 isolated from urine of a 74-year-old woman in Lódz. Serological investigation involving ELISA and Western blotting failed to classify the Proteus mirabilis strain Sm 99 into any of the 80 Proteus O serogroups. Sugar analysis along with two-dimensional NMR spectroscopy showed that the O-polysaccharide is composed of branched pentasaccharide repeating units containing one residue each of d-Glc, d-GlcNAc, d-GalNAc, d-glucuronic acid, and 4-[(R)-3-hydroxybutanoylamino]-4,6-dideoxy-d-glucose. The chemical and serological data show that the O antigen of P. mirabilis Sm 99 is unique among the known Proteus O antigens. Based on this finding, it is proposed to extend the current serological classification scheme of Proteus by adding a new serogroup, O81, which at present consists of P. mirabilis strain Sm 99 only.


Assuntos
Proteus mirabilis , Sorogrupo , Idoso , Configuração de Carboidratos , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Polissacarídeos Bacterianos/química , Polissacarídeos Bacterianos/imunologia , Proteus mirabilis/química , Proteus mirabilis/imunologia , Proteus mirabilis/isolamento & purificação
11.
Arch Virol ; 163(8): 2189-2197, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29721709

RESUMO

Four lytic Proteus bacteriophages, PM75, PM85, PM93, and PM116, which are active against multi-drug-resistant strains of P. mirabilis, were isolated from cattle and poultry samples. According to electron microscopy data, all of the investigated phages belonged to the family Podoviridae. They all demonstrated lytic activity against sensitive strains of P. mirabilis, and three of the phages, PM85, PM93, and PM116, are potential candidates for use in antibacterial treatment. The genomes and putative proteins of bacteriophages PM85, PM93, and PM116 were similar to those of Proteus phage vB_PmiP_Pm5460 [KP890822], and the investigated phages formed a distinct clade within the genus Sp6virus, subfamily Autographivirinae. The genome sequence of phage PM75 was similar to that of a previously described Proteus phage, PM16 [KF319020], and both of them demonstrated low nucleotide sequence identity to the genomes of the other most similar phages, namely, Vibrio phage VP93, Pantoea phage LIMElight, and KP34-like bacteriophages. According to cluster analysis of the complete genome sequences and phylogenetic analysis of the proteins essential for their life cycle, phages PM75 and PM16 are distinct from other similar phages from the phiKMV supergroup and should be recognized as constituting a new genus, "Pm16virus", within the subfamily Autographivirinae.


Assuntos
Bacteriófagos/isolamento & purificação , Doenças dos Bovinos/microbiologia , Podoviridae/isolamento & purificação , Doenças das Aves Domésticas/microbiologia , Infecções por Proteus/veterinária , Proteus mirabilis/virologia , Animais , Bacteriófagos/classificação , Bacteriófagos/genética , Bacteriófagos/fisiologia , Bovinos , Galinhas , Genoma Viral , Filogenia , Podoviridae/classificação , Podoviridae/genética , Podoviridae/fisiologia , Infecções por Proteus/microbiologia , Proteus mirabilis/genética , Proteus mirabilis/isolamento & purificação , Proteus mirabilis/fisiologia , Proteínas Virais/genética
12.
BMC Res Notes ; 11(1): 335, 2018 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-29788988

RESUMO

OBJECTIVE: This study aimed at assessing the magnitude, distribution, and the antimicrobial susceptibility of the extended spectrum beta-lactamase producing Entrobacteriaceae in the University of Gondar Referral Hospital environments. RESULTS: Out of a total of 384 samples, 14.8% were ESBL producing Entrobacteriaceae, where 42.10% Klebsiella pneumoniae, 35.09% Escherchia coli and 7.01% Proteus mirabilis were the predominant isolates. Most ESBL producing isolates, that is, 24.56, 22.8, and 22.8% were found from waste water, sinks and bedside tables respectively. All ESBL producing Entrobacteriaceae were found to be resistant to ceftriaxone, ceftazidime, cefpirome, cefpodoxime, and amoxicillin with Clavulanic acid. Resistance rate was also high for non-beta-lactam antimicrobials, like chloramphenicol (70.18%), cotrimoxazole (64.91%), norfloxacin (42.10%), ciprofloxacin (43.86%), and gentamicin (19.30%).


Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana , Enterobacteriaceae/isolamento & purificação , Escherichia coli/isolamento & purificação , Hospitais , Klebsiella pneumoniae/isolamento & purificação , Proteus mirabilis/isolamento & purificação , beta-Lactamases/metabolismo , Etiópia , Humanos , Testes de Sensibilidade Microbiana , Prevalência
13.
Vet Rec ; 183(1): 21, 2018 07 07.
Artigo em Inglês | MEDLINE | ID: mdl-29622684

RESUMO

The aim of this retrospective study was to document the prevalence of bacterial species in cats with significant bacteriuria and to compare their antimicrobial susceptibilities over five years. One hundred sixty-nine positive urine cultures from 150 cats were included. Fifty-five per cent showed clinical signs, while 40 per cent had subclinical bacteriuria. Escherichia coli, Staphylococcus species, Enterococcus species, Streptococcus species and Proteus mirabilis accounted for 50.5 per cent, 22.9 per cent, 15,1 per cent, 3.6 per cent and 2.6 per cent, respectively. Enterococcus species was significantly more common in cats with subclinical bacteriuria. Enterococcus species and Proteus mirabilis isolates were resistant to a significantly higher number of antimicrobials than other isolates. Applying the formula to select rational antimicrobial therapy, bacterial isolates were most likely to be susceptible to imipenem, nitrofurantoin, gentamicin and amoxicillin clavulanic acid. Over the study period, only minor differences were noted for the antimicrobial impact factors (IFs) between years and between cats with and without clinical signs. The cumulative IF increased significantly compared with the previous 10 years. Empirical treatment of bacterial cystitis should be avoided whenever possible and, if needed, based on the locally determined bacterial spectrum and antibiotic susceptibility.


Assuntos
Doenças do Gato/microbiologia , Farmacorresistência Bacteriana , Infecções Urinárias/veterinária , Animais , Antibacterianos/uso terapêutico , Doenças do Gato/tratamento farmacológico , Doenças do Gato/epidemiologia , Gatos , Enterococcus/efeitos dos fármacos , Enterococcus/isolamento & purificação , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Feminino , Masculino , Prevalência , Proteus mirabilis/efeitos dos fármacos , Proteus mirabilis/isolamento & purificação , Estudos Retrospectivos , Staphylococcus/efeitos dos fármacos , Staphylococcus/isolamento & purificação , Streptococcus/efeitos dos fármacos , Streptococcus/isolamento & purificação , Infecções Urinárias/tratamento farmacológico , Infecções Urinárias/epidemiologia , Infecções Urinárias/microbiologia
14.
Int J Antimicrob Agents ; 52(2): 287-292, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29654893

RESUMO

This study characterized the ß-lactamase content of baseline pathogens recovered from patients with complicated urinary tract infections (cUTI), including acute pyelonephritis, who were enrolled in two phase 3 clinical trials of ceftazidime-avibactam (RECAPTURE 1 and 2), and correlated the clinical efficacy of ceftazidime-avibactam and the comparator doripenem according to resistance mechanisms. A total of 26.2% (93/355) ceftazidime-avibactam and 26.8% (101/377) doripenem patients had baseline isolates that met the MIC screening criteria. The majority of Enterobacteriaceae (87.5%; 154/176) carried blaCTX-M. This pattern was mainly observed in Escherichia coli (96.8%; 92/95) and Klebsiella pneumoniae (96.0%; 48/50), whereas most Proteus mirabilis (80.0%; 8/10) carried plasmid AmpC genes. Two K. pneumoniae and 1 Klebsiella oxytoca carried blaOXA-48 and 1 K. pneumoniae carried blaNDM-1. Five (13/35; 37.1%) Pseudomonas aeruginosa isolates were screened, and 2 carbapenemase producers (IMP-18 and VIM-2) were detected. Among patients enrolled in the ceftazidime-avibactam arm who were infected by MIC screen-positive Enterobacteriaceae, clinical cure occurred in 85.7-95.5%, regardless of ß-lactamase content; the respective rate in the doripenem arm was 82.1-92.5%. A total of 75.0% in the ceftazidime-avibactam arm and 100.0% in the doripenem arm of patients infected by P. aeruginosa with MIC screen-positive criteria were clinically cured. Ceftazidime-avibactam efficacy was comparable to doripenem efficacy for treating cUTI caused by uropathogens producing extended-spectrum and/or AmpC ß-lactamases.


Assuntos
Antibacterianos/uso terapêutico , Compostos Azabicíclicos/uso terapêutico , Proteínas de Bactérias/genética , Carbapenêmicos/uso terapêutico , Ceftazidima/uso terapêutico , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Pielonefrite/tratamento farmacológico , Infecções Urinárias/tratamento farmacológico , beta-Lactamases/genética , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Proteínas de Bactérias/metabolismo , Doripenem , Método Duplo-Cego , Combinação de Medicamentos , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Escherichia coli/crescimento & desenvolvimento , Escherichia coli/isolamento & purificação , Feminino , Expressão Gênica , Infecções por Bactérias Gram-Negativas/microbiologia , Humanos , Klebsiella oxytoca/efeitos dos fármacos , Klebsiella oxytoca/genética , Klebsiella oxytoca/crescimento & desenvolvimento , Klebsiella oxytoca/isolamento & purificação , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/genética , Klebsiella pneumoniae/crescimento & desenvolvimento , Klebsiella pneumoniae/isolamento & purificação , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Plasmídeos/química , Plasmídeos/metabolismo , Proteus mirabilis/efeitos dos fármacos , Proteus mirabilis/genética , Proteus mirabilis/crescimento & desenvolvimento , Proteus mirabilis/isolamento & purificação , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/genética , Pseudomonas aeruginosa/crescimento & desenvolvimento , Pseudomonas aeruginosa/isolamento & purificação , Pielonefrite/microbiologia , Resultado do Tratamento , Infecções Urinárias/microbiologia , beta-Lactamases/metabolismo
15.
Diagn Microbiol Infect Dis ; 91(3): 284-286, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29571841

RESUMO

We describe a case of carbapenemase-harboring Proteus mirabilis together with detection of NDM-5 in Austria accompanied by other bacterial strains with a wide range of beta-lactamases including OXA-181 and VEB-6. Isolates were obtained from a subphrenic abscess from one patient who was previously treated with broad-spectrum antibiotics in Bangladesh.


Assuntos
Infecções por Proteus/microbiologia , Proteus mirabilis/enzimologia , Proteus mirabilis/isolamento & purificação , Abscesso Subfrênico/microbiologia , beta-Lactamases/metabolismo , Adulto , Áustria , Bangladesh , Humanos , Masculino , Viagem
16.
J Food Sci ; 83(3): 770-779, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29437227

RESUMO

Proteus mirabilis, a Gram-negative bacterium, is ubiquitous in the environment and is considered as the normal microflora in the human gastrointestinal tract. However, this bacterium is an opportunistic pathogen in humans, often causing urinary tract infections. Moreover, Proteus has been frequently isolated from food animals, including poultry. Whether this bacterium contributes to the foodborne illness in humans is unclear. In this report, P. mirabilis isolates recovered from broilers during housing in the units were characterized, their antimicrobial activity was assayed, and broiler immune response to the soluble proteins was determined. Cecal contents and fecal droppings were treated according to the standard protocol for isolation. Speciation based on biochemical reactions and the antimicrobial activity of the isolates were carried out using commercial kits. Immunoblot was assayed to determine immune status of broilers against P. mirabilis. A total of 10 isolates of P. mirabilis were selected for further characterization. These isolates could grow in pH 6.0 and 1% NaCl conditions. They were resistant to sodium lactate, troleandomycin, rifamycin SV, vancomycin, but sensitive to nalidixic acid, cefotaxime and novobiocin. Moreover, the CTX, ACC, CMY-1, BIC, NDM, VEB, qnrB and qnrD genes were detected by PCR amplification in all isolates. Sera from broilers harboring this bacterium reacted to the P. mirabilis soluble proteins, but not from litter- and age-matched P. mirabilis negative and SPF chickens, indicating that this bacterium infected chickens that could have humoral immune response against P. mirabilis. This study provides a rationale for further monitoring P. mirabilis during poultry production to determine whether this bacterium poses potential threats to public health.


Assuntos
Galinhas/microbiologia , Genes Bacterianos , Proteus mirabilis/isolamento & purificação , Animais , DNA Bacteriano/genética , Farmacorresistência Bacteriana Múltipla/genética , Contaminação de Alimentos , Concentração de Íons de Hidrogênio , Estrutura Molecular , Proteus mirabilis/genética , Infecções Urinárias/tratamento farmacológico , Infecções Urinárias/microbiologia
17.
BMJ Case Rep ; 20182018 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-29391357

RESUMO

A staghorn calculus is a calculus accommodating the majority of a renal calyx extending into the renal pelvis. A conservative approach to its treatment may lead to high morbidity and mortality rates. Such morbidity usually manifests with renal failure, obstructed upper urinary tractand/or life-threatening sepsis. Prostatic abscesses have never been associated with staghorn calculi in the literature. We report a case of a 70-year-old man who presented with sepsis, which was found to originate from a complex prostatic abscess. The patient had no history of urinary tract infections or risk factors. The authors believe that the incidentally identified staghorn calculi promoted the growth of Proteus mirabilis which led to the development of the prostatic abscess. The patient underwent a transurethral resection and drainage of the abscess following a failed course of antibiotic therapy. This case also highlights the paucity of guidelines available in treating prostatic abscesses.


Assuntos
Abscesso/etiologia , Doenças Prostáticas/etiologia , Doenças Prostáticas/microbiologia , Infecções por Proteus/etiologia , Cálculos Coraliformes/complicações , Abscesso/diagnóstico por imagem , Abscesso/microbiologia , Abscesso/terapia , Idoso , Antibacterianos/uso terapêutico , Humanos , Achados Incidentais , Pelve Renal , Masculino , Doenças Prostáticas/terapia , Infecções por Proteus/tratamento farmacológico , Proteus mirabilis/isolamento & purificação , Sepse/etiologia , Sepse/microbiologia , Cálculos Coraliformes/diagnóstico por imagem , Cálculos Coraliformes/microbiologia , Cálculos Coraliformes/terapia , Ressecção Transuretral da Próstata , Resultado do Tratamento
18.
BMJ Case Rep ; 20182018 Feb 16.
Artigo em Inglês | MEDLINE | ID: mdl-29453210

RESUMO

We describe a case of polymicrobial bacterial pericarditis with Klebsiella pneumoniae and Proteus mirabilis, caused by pericardial penetration of the tip of the catheter of a laparoscopic adjustable gastric band (LAGB). The patient developed a cardiac tamponade, and subsequently emergency pericardiocentesis was performed. Analysis of earlier CT scans showed that the tip of the catheter had migrated through the liver and through the diaphragm into the pericardium, and was in contact with the myocardium. After stabilisation he was operated to remove the LAGB. In this case report, we describe the chain of events that led to the polymicrobial pericarditis-a complication of LAGB placement that to our knowledge has thus far never been reported. We furthermore present a detailed literature review of all published cases of polymicrobial pericarditis and its causes.


Assuntos
Tamponamento Cardíaco/etiologia , Cateteres/efeitos adversos , Migração de Corpo Estranho/diagnóstico por imagem , Derivação Gástrica/efeitos adversos , Pericardite/etiologia , Pericárdio/lesões , Tamponamento Cardíaco/diagnóstico , Tamponamento Cardíaco/terapia , Angiografia por Tomografia Computadorizada , Humanos , Infecções por Klebsiella/diagnóstico , Infecções por Klebsiella/etiologia , Infecções por Klebsiella/terapia , Klebsiella pneumoniae/isolamento & purificação , Fígado/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Pericardiocentese , Pericardite/diagnóstico , Pericardite/terapia , Infecções por Proteus/diagnóstico , Infecções por Proteus/etiologia , Infecções por Proteus/terapia , Proteus mirabilis/isolamento & purificação , Resultado do Tratamento
19.
Enferm. infecc. microbiol. clín. (Ed. impr.) ; 36(2): 100-103, feb. 2018. graf, tab
Artigo em Espanhol | IBECS | ID: ibc-170698

RESUMO

Introducción: Se describe la epidemiología molecular de las enterobacterias productoras de carbapenemasas en un hospital terciario. Material y métodos: Se incluyeron todos los aislamientos de enterobacterias productoras de carbapenemasas obtenidos entre febrero de 2015 y marzo de 2016 en el Hospital Universitario 12 de Octubre (Madrid). Se utilizaron métodos fenotípicos y moleculares. Resultados: Se identificaron 7 especies bacterianas, predominando Klebsiella pneumoniae (K. pneumoniae) (78,9%) y Enterobacter cloacae (E. cloacae) (16,4%). La resistencia en K. pneumoniae y E. cloacae para carbapenemes fue del 88,7 y 88,6% para ertapenem, 21,4 y 54,3% para imipenem, y 20,8 y 34,3% para meropenem. El tipo de carbapenemasa más frecuente en K pneumoniae fue OXA-48 (91,1%) y en E. cloacae VIM (71,4%). Se identificaron 9 tipos clonales de K. pneumoniae, incluyendo uno mayoritario perteneciente al tipo de secuencia ST11, y 16 de E. cloacae. Conclusiones: El incremento actual de enterobacterias productoras de carbapenemasas se debe en gran medida a la diseminación de K. pneumoniae productora de OXA-48 (AU)


Introduction: A description is presented on the molecular epidemiology of carbapenemase-producing enterobacteriaceae infection in a tertiary hospital. Material and methods: A study was made on all the carbapenemase-producing enterobacteriaceae isolations obtained between February 2015 and March 2016 in the Hospital Universitario 12 de Octubre (Madrid). Phenotypic and molecular methods were used. Results: A total of 7 bacterial species were identified, with the majority being Klebsiella pneumoniae (K. pneumoniae) (78.9%) and Enterobacter cloacae (E. cloacae) (16.4%). The resistance of K. pneumoniae and E. cloacae for carbapenems was 88.7 and 88.6% for ertapenem, 21.4 and 54.3% for imipenem, and 20.8 and 34.3% for meropenem, respectively. The most frequent carbapenemase type was OXA-48 (91.1%) and VIM (71.4%) in E. cloacae. A total of 9K. pneumoniae clonal types were identified, including a majority pertaining to the sequence type ST11. In E. cloacae, 16 clonal types were identified. Conclusions: The current increase in carbapenemase-producing enterobacteriaceae is mainly due to the spread of OXA-48-producing K. pneumoniae (AU)


Assuntos
Humanos , Epidemiologia Molecular/métodos , Enterobacteriaceae/isolamento & purificação , Infecção/epidemiologia , Proteus mirabilis , Proteus mirabilis/isolamento & purificação , Infecção/microbiologia , Klebsiella pneumoniae , Klebsiella pneumoniae/isolamento & purificação , Resistência a Medicamentos
20.
Med Microbiol Immunol ; 207(2): 129-139, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29330591

RESUMO

The impact of planktonic and biofilm lifestyles of the clinical isolate Proteus mirabilis 9B-m on its lipopolysaccharide (O-polysaccharide, core region, and lipid A) was evaluated. Proteus mirabilis bacteria are able to form biofilm and lipopolysaccharide is one of the factors involved in the biofilm formation. Lipopolysaccharide was isolated from planktonic and biofilm cells of the investigated strain and analyzed by SDS-PAGE with silver staining, Western blotting and ELISA, as well as NMR and matrix-assisted laser desorption ionization time-of-flight mass spectrometry techniques. Chemical and NMR spectroscopic analyses revealed that the structure of the O-polysaccharide of P. mirabilis 9B-m strain did not depend on the form of cell growth, but the full-length chains of the O-antigen were reduced when bacteria grew in biofilm. The study also revealed structural modifications of the core region in the lipopolysaccharide of biofilm-associated cells-peaks assigned to compounds absent in cells from the planktonic culture and not previously detected in any of the known Proteus core oligosaccharides. No differences in the lipid A structure were observed. In summary, our study demonstrated for the first time that changes in the lifestyle of P. mirabilis bacteria leads to the modifications of their important virulence factor-lipopolysaccharide.


Assuntos
Biofilmes/crescimento & desenvolvimento , Lipopolissacarídeos/análise , Proteus mirabilis/química , Proteus mirabilis/crescimento & desenvolvimento , Western Blotting , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Humanos , Lipopolissacarídeos/isolamento & purificação , Espectroscopia de Ressonância Magnética , Infecções por Proteus/microbiologia , Proteus mirabilis/isolamento & purificação , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Coloração e Rotulagem
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