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1.
BMC Infect Dis ; 19(1): 565, 2019 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-31253101

RESUMO

BACKGROUND: To detect carbapenemase-producing Gram-negative bacteria in bacterial laboratories at medical settings, a new immunochromatographic assay for New Delhi metallo-ß-lactamases (NDMs) was developed. METHODS: The immunochromatographic assay for New Delhi metallo-ß-lactamases producers was developed using rat monoclonal antibodies against NDMs. The assessment was performed using 350 isolates of Gram-negative bacteria, including Acinetobacter baumannii (51 isolates), Enterobacteriaceae (163 isolates), and Pseudomonas aeruginosa (136 isolates) obtained from 2015 to 2017 in medical settings in Myanmar. Of them, 302 isolates were resistant to carbapenems, including imipenem and/or meropenem. The blaNDM genes were identified by PCR and sequencing. RESULTS: Of the 350 clinical isolates tested, 164 (46.9%) (60 isolates of Escherichia coli, 51 isolates of Klebsiella pneumoniae, 25 isolates of Enterobacter cloacae, 23 isolates of P. aeruginosa, and 5 isolates of A. baumannii) were positive on this assay, and all the positive isolates harbored genes encoding NDM-1, - 4, - 5 and - 7. The remaining 186 (53.1%) isolates negative on the assay did not harbor genes encoding NDMs. The assay had a specificity of 100% and a sensitivity of 100%. The assessment revealed that more than 90% of carbapenem-resistant Enterobacteriaceae produced NDMs. CONCLUSIONS: The immunochromatographic assay is an easy-to-use and reliable kit for detection of NDMs-producing Gram-negative bacteria. The assay revealed that NDM-producing Enterobacteriaceae isolates are wide-spread in medical settings in Myanmar.


Assuntos
Bactérias Gram-Negativas/isolamento & purificação , Imunoensaio/métodos , beta-Lactamases/imunologia , Acinetobacter baumannii/enzimologia , Acinetobacter baumannii/isolamento & purificação , Animais , Antibacterianos/farmacologia , Anticorpos Monoclonais/imunologia , Farmacorresistência Bacteriana , Enterobacteriaceae/enzimologia , Enterobacteriaceae/isolamento & purificação , Bactérias Gram-Negativas/enzimologia , Bactérias Gram-Negativas/genética , Humanos , Klebsiella pneumoniae/enzimologia , Klebsiella pneumoniae/isolamento & purificação , Testes de Sensibilidade Microbiana , Mianmar , Pseudomonas aeruginosa/enzimologia , Pseudomonas aeruginosa/isolamento & purificação , Ratos , beta-Lactamases/genética , beta-Lactamases/metabolismo
2.
Anal Bioanal Chem ; 411(17): 3829-3838, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31172234

RESUMO

The rapid detection of bacterial strains has become a major topic thoroughly discussed across the biomedical field. Paired with the existence of nosocomial pathogen agents that imply extreme medical and financial challenges throughout diagnosis and treatment, the development of rapid and easy-to-use sensing devices has gained an increased amount of attention. Moreover, antibiotic resistance considered by World Health Organization as one of the "biggest threats to global health, food security, and development today" enables this topic as high priority. Pseudomonas aeruginosa, one of the most ubiquitous bacterial strains, has various quorum-sensing systems that are a direct cause of their virulence. One of them is represented by pyocyanin, a blue pigment with electroactive properties that is synthesized from early stages of bacterial colonization. Thus, the sensitive detection of this biomarker could enable a personalized and efficient therapy. It was achieved with the development of an electrochemical sensor based on a thermosensitive polymer, modified with Au/Ag nanoalloy for the rapid and accurate detection of pyocyanin, a virulence biomarker of Pseudomonas aeruginosa. The sensor displayed a linear range from 0.12 to 25 µM, and a limit of detection of 0.04 µM (signal/noise = 3). It was successfully tested in real samples spiked with the target analyte without any pretreatment other than a dilution step. The detection of pyocyanin with high recovery in whole blood in a time frame of 5-10 min from the moment of collection was performed with this electrochemical sensor. Graphical abstract.


Assuntos
Ligas/química , Ouro/química , Hidrogéis/química , Nanopartículas Metálicas/química , Piocianina/análise , Prata/química , Ágar/química , Artefatos , Biomarcadores/análise , Técnicas Biossensoriais , Técnicas Eletroquímicas/métodos , Limite de Detecção , Sistemas Automatizados de Assistência Junto ao Leito , Pseudomonas aeruginosa/isolamento & purificação , Piocianina/sangue , Reprodutibilidade dos Testes , Temperatura Ambiente
3.
J Photochem Photobiol B ; 197: 111510, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31163288

RESUMO

Treatment of chronic lung infection becomes a great challenge due to the drug resistant bacteria. In this scenario, evolving a new drug based on lipid metal conjugation loaded with potential antibiotic provides better drug delivery. In this study, ciprofloxacin loaded selenium-lipid nanoparticle (CxLSENPs) is produced in a novel route and its antimicrobial properties were tested against clinically important Gram-negative P. aeruginosa. The synthesized CxLSENPs was characterized by biophysical techniques (UV, Fluorescence spectroscopy, Raman spectroscopy, FTIR, FESEM, HRTEM and Zeta potential). Raman spectra coupled with FTIR spectra confirmed the possible interaction of lipid components in the NPs. HRTEM analysis confirmed the spherical shape of NPs. CxLSENPs recorded greater antibacterial effects on P. aeruginosa. A drastic reduction in the count of P. aeruginosa was observed after treatment with CxLSENPs. In order to further confirm the antibacterial efficiency, the live/dead cell assay was carried out. Live/dead analysis helps us to investigate the viability of bacterial cells. The number of dead bacterial cells was significantly higher in CxLSENPs treated groups when compare to the control. Furthermore, CxLSENPs increased the antioxidant enzyme activities (SOD, GPx, CAT and LPO) in mouse and protected the liver damage from bacterial infection. This study concludes that the developed CxLSENPs might be employed as strong antimicrobial and antioxidant agents for treating lung infection or interstitial lung diseases.


Assuntos
Antibacterianos/química , Ciprofloxacino/química , Portadores de Fármacos/síntese química , Lipídeos/química , Nanopartículas/química , Selênio/química , Animais , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Antioxidantes/metabolismo , Infecções Bacterianas/microbiologia , Infecções Bacterianas/prevenção & controle , Catalase/metabolismo , Ciprofloxacino/farmacologia , Ciprofloxacino/uso terapêutico , Portadores de Fármacos/química , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Doenças Pulmonares Intersticiais/tratamento farmacológico , Doenças Pulmonares Intersticiais/microbiologia , Doenças Pulmonares Intersticiais/patologia , Masculino , Camundongos , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/isolamento & purificação , Superóxido Dismutase/metabolismo
5.
BMC Infect Dis ; 19(1): 411, 2019 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-31088389

RESUMO

BACKGROUND: Colorectal surgery is associated with high rates of surgical site infection (SSI). We investigated SSI in radical resection of colon or rectal carcinoma and its epidemiological distribution in 26 hospitals in China. METHODS: We conducted prospective surveillance of patients who underwent radical resection of colon or rectal carcinoma in 26 selected hospitals from January 2015 to June 2016.An information system monitored all of the surgical inpatients. Infection control professionals observed the inpatients with suspected SSI who had been screened by the system at the bedside. The infection status of the incisions was followed up by telephone 1 month after the operation. RESULTS: In total, 5729 patients were enrolled for the two operations; SSIs occurred in 206 patients, and the infection rate was 3.60%. The incidence of SSI after radical resection of rectal carcinoma (5.12%; 119/2323) was 2.1 times higher than that after radical resection of colon carcinoma (2.55%; 87/3406) (P < 0.0001). Additionally, in the colon versus rectal groups, the rate of superficial incisional SSI was 0.94% versus 2.28% (P < 0.0001), the rate of deep incisional SSI was 0.56% versus 1.11% (P = 0.018), and the rate of organ space SSI was 1.06% versus 1.72% (P = 0.031), respectively. The most common pathogens causing SSIs after radical resection of colon carcinoma were Escherichia coli (21/38) and Pseudomonas aeruginosa (5/38). Escherichia coli (24/65) and Enterococcus spp. (14/65) were the two most common pathogens in the rectal group. The multivariate logistic regression analysis showed that only the operating time and number of hospital beds were common independent risk factors for SSIs after the two types of surgery. CONCLUSION: This multicenter study showed that there were significant differences in the incidence of SSIs, three types of SSIs, and some risk factors between radical resection of colon carcinoma and rectal carcinoma.


Assuntos
Neoplasias do Colo/cirurgia , Neoplasias Retais/cirurgia , Infecção da Ferida Cirúrgica/diagnóstico , Idoso , China/epidemiologia , Escherichia coli/isolamento & purificação , Feminino , Número de Leitos em Hospital , Humanos , Incidência , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Duração da Cirurgia , Estudos Prospectivos , Pseudomonas aeruginosa/isolamento & purificação , Fatores de Risco , Infecção da Ferida Cirúrgica/epidemiologia , Infecção da Ferida Cirúrgica/microbiologia
6.
BMC Res Notes ; 12(1): 244, 2019 Apr 29.
Artigo em Inglês | MEDLINE | ID: mdl-31036061

RESUMO

OBJECTIVES: Diabetic foot ulcers (DFUs) often lead to hospital admissions, amputations and deaths; however, there is no up-to-date information on microbial isolates from DFUs and no mention of utilization of molecular techniques in Sub-Saharan Africa. We conducted a cross-sectional study among 83 adult patients at a tertiary hospital in Kenya over 12 months. The study aimed to isolate, identify bacteria, their antibiotic susceptibility patterns in active DFUs, and to compare standard microbiological methods versus a real-time PCR commercial kit in the detection of Staphylococcus aureus DNA and methicillin-resistant S. aureus (MRSA) DNA. RESULTS: Eighty swabs (94%) were culture-positive; 29% were Gram-positive and 65% were Gram-negative. The main organisms isolated were S. aureus (16%), Escherichia coli (15%), Proteus mirabilis (11%), Klebsiella pneumoniae (7%) and Pseudomonas aeruginosa (7%). The bacterial isolates showed resistance to commonly used antibiotics such as ampicillin, amoxicillin, cefepime, ceftazidime, cefuroxime, clindamycin, erythromycin, piperacillin-tazobactam, tetracycline and trimethoprim-sulphamethoxazole (TMPSMX). Thirty-one percent of the S. aureus isolated and 40% of the Gram-negatives were multi-drug resistant organisms (MDROs). There was a high prevalence of nosocomial bacteria. MRSA were not identified using culture methods but were identified using PCR. PCR was more sensitive but less specific than culture-based methods to identify S. aureus.


Assuntos
Antibacterianos/uso terapêutico , Infecções Bacterianas/diagnóstico , Pé Diabético/diagnóstico , Farmacorresistência Bacteriana Múltipla , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Infecções Bacterianas/tratamento farmacológico , Infecções Bacterianas/epidemiologia , Infecções Bacterianas/microbiologia , Técnicas de Tipagem Bacteriana , Cefalosporinas/uso terapêutico , Clindamicina/uso terapêutico , Estudos Transversais , Pé Diabético/tratamento farmacológico , Pé Diabético/epidemiologia , Pé Diabético/microbiologia , Escherichia coli/classificação , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Escherichia coli/isolamento & purificação , Humanos , Quênia/epidemiologia , Klebsiella pneumoniae/classificação , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/genética , Klebsiella pneumoniae/isolamento & purificação , Macrolídeos/uso terapêutico , Staphylococcus aureus Resistente à Meticilina/classificação , Staphylococcus aureus Resistente à Meticilina/genética , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Testes de Sensibilidade Microbiana , Penicilinas/uso terapêutico , Proteus mirabilis/classificação , Proteus mirabilis/efeitos dos fármacos , Proteus mirabilis/genética , Proteus mirabilis/isolamento & purificação , Pseudomonas aeruginosa/classificação , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/genética , Pseudomonas aeruginosa/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real , Staphylococcus aureus/classificação , Staphylococcus aureus/genética , Staphylococcus aureus/isolamento & purificação , Sulfanilamidas/uso terapêutico
7.
Eur J Med Chem ; 175: 63-71, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31075609

RESUMO

In the frame of a research program aimed to explore the relationship between chirality of iminosugars and their therapeutic potential, herein we report the synthesis of N-akyl l-deoxyiminosugars and the evaluation of the anti-inflammatory properties of selected candidates for the treatment of Pseudomonas aeruginosa infections in Cystic Fibrosis (CF) lung disease. Target glycomimetics were prepared by the shortest and most convenient approach reported to date, relying on the use of the well-known PS-TPP/I2 reagent system to prepare reactive alkoxyalkyl iodides, acting as key intermediates. Iminosugars ent-1-3 demonstrated to efficiently reduce the inflammatory response induced by P. aeruginosa in CuFi cells, either alone or in synergistic combination with their d-enantiomers, by selectively inhibiting NLGase. Surprisingly, the evaluation in murine models of lung disease showed that the amount of ent-1 required to reduce the recruitment of neutrophils was 40-fold lower than that of the corresponding d-enantiomer. The remarkably low dosage of the l-iminosugar, combined with its inability to act as inhibitor for most glycosidases, is expected to limit the onset of undesired effects, which are typically associated with the administration of its d-counterpart. Biological results herein obtained place ent-1 and congeners among the earliest examples of l-iminosugars acting as anti-inflammatory agents for therapeutic applications in Cystic Fibrosis.


Assuntos
Antibacterianos/uso terapêutico , Fibrose Cística/complicações , Imino Açúcares/uso terapêutico , Infecções por Pseudomonas/complicações , Infecções por Pseudomonas/tratamento farmacológico , Pseudomonas aeruginosa/isolamento & purificação , Animais , Antibacterianos/administração & dosagem , Antibacterianos/química , Antibacterianos/farmacologia , Anti-Inflamatórios/administração & dosagem , Anti-Inflamatórios/farmacologia , Brônquios/imunologia , Brônquios/microbiologia , Brônquios/patologia , Relação Dose-Resposta a Droga , Humanos , Imino Açúcares/administração & dosagem , Imino Açúcares/química , Imino Açúcares/farmacologia , Inflamação/prevenção & controle , Concentração Inibidora 50 , Camundongos , Neutrófilos/imunologia , Infecções por Pseudomonas/microbiologia , Pseudomonas aeruginosa/efeitos dos fármacos , Estereoisomerismo , beta-Glucosidase/antagonistas & inibidores
8.
R I Med J (2013) ; 102(3): 38-41, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30943671

RESUMO

A 59-year-old man presented with sharp chest pain, hypoxia, and tachycardia. His past medical history included intravenous drug use (IVDU). Pseudomonas aeruginosa was isolated from his blood. Pseudomonas aeruginosa is a rare cause of endocarditis. Patient revealed that he injected drugs intravenously with tap water. Transesophageal echocardiogram revealed vegetation on the anterior mitral leaflet with associated mitral regurgitation. Patient was successfully treated with meropenem and tobramycin and underwent mitral valve replacement without complications. Majority of IVDU-related endocarditis caused by Pseudomonas involve right-sided valves, but our case is unique as it demonstrates left-sided endocarditis in a patient with IVDU. A combination of aggressive medical and early surgical treatment with valve replacement has enabled this patient to successfully recuperate.


Assuntos
Endocardite Bacteriana/diagnóstico , Infecções por Pseudomonas/diagnóstico , Pseudomonas aeruginosa/isolamento & purificação , Antibacterianos/uso terapêutico , Ecocardiografia Transesofagiana , Endocardite Bacteriana/tratamento farmacológico , Endocardite Bacteriana/cirurgia , Próteses Valvulares Cardíacas , Humanos , Masculino , Pessoa de Meia-Idade , Infecções por Pseudomonas/tratamento farmacológico , Abuso de Substâncias por Via Intravenosa/complicações
9.
Trials ; 20(1): 215, 2019 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-30975204

RESUMO

BACKGROUND: There are many comorbidities associated with Down syndrome (DS), including obstructive sleep apnea (OSA) and masticatory muscle alteration. Muscular hypotonia, in particular, of the masticatory and oropharyngeal muscles is one of the main characteristics of individuals with DS, resulting in impairments of speech, swallowing, and mastication in these individuals. In addition, total or partial obstruction of the airways during sleep can occur due to pharyngeal hypotonia, leading to snoring and to OSA. This progressive respiratory disorder is associated with a high risk of morbidity and mortality in individuals with DS. The aim of this research is to assess the therapeutic effects of surface neuromuscular electrical stimulation (NMES), the mastication apparatus (MA), and a mandibular advancement oral appliance (OAm) with an embedded thermosensitive microchip on the functions of masticatory muscles (bilateral masseter and temporal muscles), physiological sleep variables, and salivary parameters in adult patients with DS. METHODS: The patients with DS will be randomly selected and divided into three groups (DS-NMES, DS-MA, and DS-OAm) with a minimum of 10 patients in each group. A thermosensitive microchip will be embedded in the OAm to record its compliance. The therapeutic effects on masticatory muscle function will be investigated through electromyography, a caliper, and a force-transducer device; the sleep variables, in turn, will be evaluated by means of polysomnography. The physicochemical and microbiological properties of the saliva will also be analyzed, including the salivary flow, viscosity, buffer capacity, cortisol levels (susceptibility to psychological and/or physical stress), and Pseudomonas aeruginosa levels (risk of aspiration pneumonia) in these patients. The methods determined for this study will be carried out prior to and after 2 months of the recommended therapies. DISCUSSION: The primary outcomes would be the improvement and/or reestablishment of the function of masticatory muscles and the physiological sleep variables in this target public since individuals with DS commonly present generalized muscular hypotonia and dysfunction of the oropharyngeal musculature. As a secondary outcome indicator, the impact of the applied therapies (NMES, MA, and OAm) on the salivary microbiological and physicochemical properties in DS individuals will also be assessed. Furthermore, the compliance of OAm usage will be measured through a thermosensitive microchip. TRIAL REGISTRATION: Registro Brasileiro de Ensaios Clínicos, RBR-3qp5np . Registered on 20 February 2018.


Assuntos
Síndrome de Down/terapia , Terapia por Estimulação Elétrica , Músculos da Mastigação/fisiopatologia , Saliva/microbiologia , Sono/fisiologia , Adolescente , Adulto , Síndrome de Down/fisiopatologia , Eletromiografia , Humanos , Hidrocortisona/análise , Pseudomonas aeruginosa/isolamento & purificação , Saliva/química , Tamanho da Amostra , Adulto Jovem
10.
Sensors (Basel) ; 19(8)2019 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-31003541

RESUMO

One concern to the patients is the off-line detection of pneumonia infection status after using the ventilator in the intensive care unit. Hence, machine learning methods for ventilator-associated pneumonia (VAP) rapid diagnose are proposed. A popular device, Cyranose 320 e-nose, is usually used in research on lung disease, which is a highly integrated system and sensor comprising 32 array using polymer and carbon black materials. In this study, a total of 24 subjects were involved, including 12 subjects who are infected with pneumonia, and the rest are non-infected. Three layers of back propagation artificial neural network and support vector machine (SVM) methods were applied to patients' data to predict whether they are infected with VAP with Pseudomonas aeruginosa infection. Furthermore, in order to improve the accuracy and the generalization of the prediction models, the ensemble neural networks (ENN) method was applied. In this study, ENN and SVM prediction models were trained and tested. In order to evaluate the models' performance, a fivefold cross-validation method was applied. The results showed that both ENN and SVM models have high recognition rates of VAP with Pseudomonas aeruginosa infection, with 0.9479 ± 0.0135 and 0.8686 ± 0.0422 accuracies, 0.9714 ± 0.0131, 0.9250 ± 0.0423 sensitivities, and 0.9288 ± 0.0306, 0.8639 ± 0.0276 positive predictive values, respectively. The ENN model showed better performance compared to SVM in the recognition of VAP with Pseudomonas aeruginosa infection. The areas under the receiver operating characteristic curve of the two models were 0.9842 ± 0.0058 and 0.9410 ± 0.0301, respectively, showing that both models are very stable and accurate classifiers. This study aims to assist the physician in providing a scientific and effective reference for performing early detection in Pseudomonas aeruginosa infection or other diseases.


Assuntos
Nariz Eletrônico , Pneumonia Associada à Ventilação Mecânica/diagnóstico , Infecções por Pseudomonas/diagnóstico , Adulto , Feminino , Humanos , Unidades de Terapia Intensiva , Aprendizado de Máquina , Masculino , Pneumonia Associada à Ventilação Mecânica/complicações , Pneumonia Associada à Ventilação Mecânica/microbiologia , Pneumonia Associada à Ventilação Mecânica/fisiopatologia , Infecções por Pseudomonas/complicações , Infecções por Pseudomonas/microbiologia , Infecções por Pseudomonas/fisiopatologia , Pseudomonas aeruginosa/isolamento & purificação , Pseudomonas aeruginosa/patogenicidade
11.
BMJ Case Rep ; 12(4)2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30940665

RESUMO

A 74-year-old woman presented with dyspnoea and fever on a background of a large hepatic cyst thought to be stable in size. During her admission, she developed worsening shortness of breath. An echocardiogram revealed severely impaired cardiac output due to external compression by the hepatic cyst, which had grown rapidly. This was drained under ultrasound guidance and cultures of this fluid grew Pseudomonas aeruginosa: the organism responsible for the persistence of this giant hepatic cyst.


Assuntos
Baixo Débito Cardíaco/etiologia , Cistos/complicações , Hepatopatias/complicações , Infecções por Pseudomonas/complicações , Idoso , Antibacterianos/uso terapêutico , Cistos/diagnóstico por imagem , Drenagem , Dispneia/etiologia , Feminino , Humanos , Hepatopatias/diagnóstico por imagem , Metronidazol/uso terapêutico , Piperacilina/uso terapêutico , Infecções por Pseudomonas/diagnóstico , Infecções por Pseudomonas/tratamento farmacológico , Pseudomonas aeruginosa/isolamento & purificação , Tazobactam/uso terapêutico , Tomografia Computadorizada por Raios X , Ultrassonografia de Intervenção
12.
Future Microbiol ; 14: 609-622, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30995873

RESUMO

Aim: This research pioneers the process of obtaining information concerning the distribution and existence of seven ESBL genes linked to Pseudomonas, three virulence and five quorum sensing separated from 100 camel meat samples using PCR. Materials & methods: The Vitek system was used to identify Pseudomonas species. Phenotypic antibiotic resistance of 16 antibiotics was tested by disc diffusion. Quantification of pyocyanin, elastase, alkaline protease, biofilm and Vero cell cytotoxicity was also implemented. Results: The total number of Pseudomonas species isolated from camel meat was 10/100 identified as Pseudomonas aeruginosa 8/10, Pseudomonas fluorescens 2/10. The isolates were multidrug resistant and were resistant to four to eight antibiotics representing four to six classes. The 15 genes exhibited a huge diversity in their association. Conclusion: The results indicated that camel meat is an unpropitious hotbed for Pseudomonas species of clinical significance.


Assuntos
Biofilmes/crescimento & desenvolvimento , Farmacorresistência Bacteriana Múltipla/genética , Carne/microbiologia , Infecções por Pseudomonas/microbiologia , Infecções por Pseudomonas/veterinária , Pseudomonas/efeitos dos fármacos , Pseudomonas/genética , Percepção de Quorum/genética , Fatores de Virulência/genética , Animais , Antibacterianos/farmacologia , Proteínas de Bactérias , Camelus , Cercopithecus aethiops , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão , Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos , Egito/epidemiologia , Endopeptidases , Microbiologia de Alimentos , Genes Bacterianos/genética , Elastase Pancreática , Reação em Cadeia da Polimerase/métodos , Pseudomonas/classificação , Pseudomonas/isolamento & purificação , Infecções por Pseudomonas/epidemiologia , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/isolamento & purificação , Pseudomonas fluorescens/efeitos dos fármacos , Pseudomonas fluorescens/isolamento & purificação , Piocianina , Células Vero , Virulência/genética , beta-Lactamases/genética
13.
BMJ Case Rep ; 12(3)2019 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-30936347

RESUMO

A 26-year-old healthy patient had a fever and chest pain three days after nasal carbon dioxide (CO2) laser surgery for chronic and allergic rhinitis. In the emergency room, he was diagnosed as a right pneumothorax and managed as outpatients with oral antibiotic therapy and close follow-up. Six days later, in follow-up clinic, his presenting signs and symptoms included right chest pain, tachypnoea and elevated levels of white blood cell count and C reactive protein. He was diagnosed as septic pulmonary embolism (SPE) by the detection of multiple nodules with cavitation on chest CT. Culture of pleural fluids showed Pseudomonas aeruginosa Intravenous antibiotic treatment and drainage of the pleural effusion improved his condition. Since SPE occurred after nasal CO2 laser surgery in this case, careful attention should be paid to infectious complications of nasal CO2 laser surgery.


Assuntos
Antibacterianos/uso terapêutico , Lasers de Gás/efeitos adversos , Infecções por Pseudomonas/diagnóstico , Pseudomonas aeruginosa/isolamento & purificação , Embolia Pulmonar/microbiologia , Rinite/cirurgia , Sepse/diagnóstico , Adulto , Infecção Hospitalar , Humanos , Masculino , Infecções por Pseudomonas/complicações , Infecções por Pseudomonas/tratamento farmacológico , Embolia Pulmonar/tratamento farmacológico , Embolia Pulmonar/fisiopatologia , Sepse/complicações , Sepse/tratamento farmacológico , Tomografia Computadorizada por Raios X , Resultado do Tratamento
14.
World J Microbiol Biotechnol ; 35(3): 47, 2019 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-30834977

RESUMO

The present study was aimed to assess the scope of native potential endophyte Pseudomonas aeruginosa (LSE-2) strain (KX925973) with recommended Bradyrhizobium sp. (LSBR-3) (KF906140) for synergistic effect to develop as consortium biofertilizer of soybean. A total of 28 non-rhizobial endophytic bacteria were isolated from cultivated and wild sp. of soybean. All isolates were screened for multifarious PGP traits viz. Indole-3-acetic acid (IAA), phosphate (P) and zinc (Zn) solubilization, siderophore, cell wall degrading enzymes and pathogenicity. Compatible of LSBR-3 and LSE-2 enhanced IAA, P-solubilization, 1-aminocyclopropane-carboxylate deaminase and biofilm formation over the single inoculant treatment. Further, consortium was evaluated in vivo for growth, symbiotic traits, nutrient acquisition, soil quality parameters and yield attributes of soybean. Improvement in growth parameters were recorded with dual inoculant LSBR-3 + LSE-2 as compared to LSBR-3 alone and un-inoculated control treatments. Significantly (p ≥ 0.05) high symbiotic and soil quality parameters (phosphatase and soil dehydrogenase activity) was recorded with LSBR-3 + LSE-2 at vegetative and flowering stage as compared to LSBR-3 alone and un-inoculated control treatments. Single inoculation of LSBR-3 improved grain yield by 4.25% over the un-inoculated control treatment, further, enhancement in yield was recorded with consortium inoculant (LSBR-3 and LSE-2) by 3.47% over the LSBR-3 alone. Application of consortium inoculant (LSBR-3 + LSE-2) gave an additional income of Rs. 5089/ha over the un-inoculated control treatment. The results, thus strongly suggest that endophytic diazotroph LSE-2 can be used as potent bio-inoculant along with LSBR-3 as bio-enhancer for improving soybean productivity in a sustainable system.


Assuntos
Bradyrhizobium/fisiologia , Endófitos , Nutrientes , Desenvolvimento Vegetal , Pseudomonas aeruginosa/fisiologia , Soja/crescimento & desenvolvimento , Soja/microbiologia , Simbiose , Biofilmes/crescimento & desenvolvimento , Bradyrhizobium/isolamento & purificação , Índia , Ácidos Indolacéticos/metabolismo , Nitrogênio/análise , Oxirredutases/análise , Fosfatos/metabolismo , Monoéster Fosfórico Hidrolases/análise , Filogenia , Raízes de Plantas/química , Raízes de Plantas/microbiologia , Brotos de Planta/química , Brotos de Planta/microbiologia , Potássio/análise , Pseudomonas aeruginosa/classificação , Pseudomonas aeruginosa/genética , Pseudomonas aeruginosa/isolamento & purificação , RNA Ribossômico 16S/genética , Sideróforos/metabolismo , Solo/química , Microbiologia do Solo , Solubilidade , Virulência , Zinco/metabolismo
15.
Artigo em Inglês | MEDLINE | ID: mdl-30912480

RESUMO

Urinary tract infections (UTIs) have been frequently reported from different parts of the world. The current knowledge on distribution of causative agents of urinary infections and antibiotics susceptibility pattern is essentially required. In the present study, total 351 uropathogenic bacteria were isolated; among them most prevalent were Escherichia coli (75%), followed by Pseudomonas aeruginosa (8%), Proteus mirabilis (6%), Klebsiella pneumoniae (4%), Staphylococcus aureus (4%) and Enterococcus faecalis (3%). Most isolates of uropathogenic bacteria showed resistance to amoxicillin and trimethoprim, followed by chloramphenicol and kanamycin. Biosynthesis of sulfur nanoparticles (SNPs) was performed by co-precipitation method using sodium thiosulfate in presence of Catharanthus roseus leaf extract. The characterization data showed that SNPs were polydispersed, spherical in shape with size range of 20-86 nm and having negative zeta potential of -9.24 mV. The potential antibacterial activity was observed for SNPs alone and in combination with antibiotics particularly amoxicillin and trimethoprim against majority of the uropathogens. The synergistic effect yielded increase in fold area with high activity index against tested uropathogens. Based on overall results, it can be recommended to use SNPs for the management of UTI alone and also in combination with antibiotics.


Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos , Nanopartículas/química , Enxofre/farmacologia , Infecções Urinárias/microbiologia , Antibacterianos/química , Sinergismo Farmacológico , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Humanos , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/isolamento & purificação , Testes de Sensibilidade Microbiana , Proteus mirabilis/efeitos dos fármacos , Proteus mirabilis/isolamento & purificação , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/isolamento & purificação , Enxofre/química
16.
Bioresour Technol ; 281: 421-428, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30849698

RESUMO

Although polycyclic aromatic hydrocarbons (PAHs) are considered as toxic and refractory pollutants, their biodegradation can be facilitated by biosurfactants. However, few studies have been performed to understand the potential isolation and application of biosurfactant-producing microorganism for promoting the in-situ removal of PAHs from wastewaters. In this work, a biosurfactant-producing strain S5 isolated from coking wastewater was identified as Pseudomonas aeruginosa. The biosurfactant produced by strain S5 was determined as glycolipid with a critical micelle concentration (CMC) of 96.5 mg/L, and reduced the surface tension from 72.2 to 29.6 mN/m. Addition indigenous P. aeruginosa S5 to coking wastewater effectively promoted the biodegradation of high weight molecular (HWM) PAHs (reduction from 9141.02 to 5117.16 µg/L in 15 days) in sludge phase. The results showed that the removal of PAHs in the sludge was enhanced by inoculating indigenous biosurfactant-producing microorganism in coking wastewater serving as an in-site remediation technology.


Assuntos
Coque , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Pseudomonas aeruginosa/metabolismo , Águas Residuárias/microbiologia , Biodegradação Ambiental , Recuperação e Remediação Ambiental , Pseudomonas aeruginosa/isolamento & purificação , Tensoativos/metabolismo
17.
Biosens Bioelectron ; 132: 224-229, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-30877887

RESUMO

Rapid detection of Pseudomonas aeruginosa (P. aeruginosa) is of great importance for accurate diagnosis and treatment of infected patients. In this study, a novel method was developed for the selective detection of P. aeruginosa by combing the sandwich type complex of magnetic bead/aptamer/polyadenylated-DNA with the sensitive detection platform of gold (Au) interdigital electrode connected to a multichannel series piezoelectric quartz crystal (Au IDE-MSPQC) system. Here, the magnetic bead (MB) was used as carrier for immobilization of the aptamer of P. aeruginosa. Polyadenylated DNA was bound to the aptamer through complementary strand pairing. When the P. aeruginosa was present in the sample solution, the polyadenylated DNA was replaced by the P. aeruginosa because of the specific interaction between P. aeruginosa and its aptamer. The released polyadenylated DNA strand in the detected solution could adsorb onto the surface of Au IDE by virtue of the strong interaction between adenine (A) and Au IDE, and result in sensitive frequency shift response of the MSPQC sensor. The limits of detection (LOD) of the method were as low as 9 CFU/mL in buffer and 52 CFU/mL in simulated blood sample. The proposed method was successfully applied to the selective detection of P. aeruginosa in blood samples. The constructed sensor is expected to find application for the rapid detection of P. aeruginosa in environment, food and clinical diagnosis.


Assuntos
Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais/instrumentação , Técnicas Eletroquímicas/instrumentação , Ouro/química , Poli A/química , Infecções por Pseudomonas/microbiologia , Pseudomonas aeruginosa/isolamento & purificação , Técnicas Biossensoriais/métodos , Técnicas Eletroquímicas/métodos , Eletrodos , Monitoramento Ambiental/instrumentação , Monitoramento Ambiental/métodos , Desenho de Equipamento , Análise de Alimentos/instrumentação , Análise de Alimentos/métodos , Humanos , Ácidos Nucleicos Imobilizados/química , Limite de Detecção , Imãs/química , Infecções por Pseudomonas/diagnóstico
18.
Int J Antimicrob Agents ; 53(6): 774-780, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30831233

RESUMO

Pseudomonas aeruginosa is one of the most important pathogens in cystic fibrosis. This study was conducted to analyse the genetic basis and phylogenetic profile of resistance to ceftazidime/avibactam, ceftolozane/tazobactam and carbapenems in cystic fibrosis P. aeruginosa isolates. Whole genome sequence analysis was conducted of isolates resistant to piperacillin/tazobactam collected from seven hospitals in Scotland since the introduction of these two cephalosporin/ß-lactamase inhibitor combinations. Ceftazidime resistance was primarily related to AmpC induction, as tested by cloxacillin inhibition assays, while high-level ceftazidime resistance not reversed by cloxacillin was associated with amino acid variations in AmpC. Only isolates resistant to both ceftazidime/avibactam and ceftolozane/tazobactam carried AmpD mutations, likely resulting in ampC overexpression. All isolates resistant to ceftazidime/avibactam and/or ceftolozane/tazobactam were resistant to carbapenems and showed inactivating mutations in the chromosomal oprD gene. None of the isolates bore class A, B, D plasmid-encoded carbapenemases. This study showed that mutational resistance emerged in phylogenetically distant lineages, which indicates the mutations occur independently without conferring a selective advantage to any phylogenetic lineage. These findings confirm the strong contribution of mutation-driven evolution to the population structure of P. aeruginosa.


Assuntos
Antibacterianos/farmacologia , Compostos Azabicíclicos/farmacologia , Carbapenêmicos/farmacologia , Ceftazidima/farmacologia , Cefalosporinas/farmacologia , Farmacorresistência Bacteriana , Combinação Piperacilina e Tazobactam/farmacologia , Infecções por Pseudomonas/microbiologia , Pseudomonas aeruginosa/efeitos dos fármacos , Tazobactam/farmacologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/genética , Fibrose Cística/complicações , Combinação de Medicamentos , Feminino , Hospitais , Humanos , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Porinas/genética , Pseudomonas aeruginosa/genética , Pseudomonas aeruginosa/isolamento & purificação , Escócia , Sequenciamento Completo do Genoma , Inibidores de beta-Lactamases/farmacologia , beta-Lactamases/biossíntese , beta-Lactamases/genética
19.
Int J Antimicrob Agents ; 53(5): 698-702, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30880231

RESUMO

Multi-drug resistance among Pseudomonas aeruginosa in hospitals, and particularly intensive care units, has achieved alarming rates. Some combination antimicrobial therapies have demonstrated promising synergistic effects and an ability to overcome resistance without increasing drug-related toxicities. Nevertheless, rapid and feasible methods to identify synergy have not been routinely implemented in clinical microbiology laboratories. Synergistic activity of meropenem plus tobramycin or levofloxacin against clinical P. aeruginosa isolates (N=21) was assessed by two different methods using gradient diffusion strips (GDSs). A 90° angle was created at the intersection of the minimum inhibitory concentration (MIC) of each drug by the standard method, and by a novel method, the cross was placed at clinically relevant steady-state concentrations (Css) based on recommended dosing regimens. Fractional inhibitory concentration indexes were determined to describe antibiotic interactions. Time-kill analyses were performed over 24 h in duplicate for instances of discordance between the standard cross method and the novel method. Synergy between meropenem and tobramycin by the novel method was observed in one (4.8%) isolate and between meropenem and levofloxacin in two (9.5%) isolates. Agreement with the standard method was 86-100% for meropenem plus tobramycin and meropenem plus levofloxacin combinations, respectively. Time-kill studies resulted in agreement with GDSs crossed at Css in two of three instances of discordance between GDS methods. This novel method of synergy testing that involves crossing GDSs at steady-state concentrations may be a rapid and feasible tool for routine practice. Further comparisons of this novel procedure with time-kill methods are needed.


Assuntos
Antibacterianos/farmacologia , Sinergismo Farmacológico , Meropeném/farmacologia , Testes de Sensibilidade Microbiana/métodos , Pseudomonas aeruginosa/efeitos dos fármacos , Tobramicina/farmacologia , Humanos , Infecções por Pseudomonas/microbiologia , Pseudomonas aeruginosa/isolamento & purificação
20.
Analyst ; 144(8): 2803-2810, 2019 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-30882113

RESUMO

Colistin is recognized as the last therapeutic option for multidrug-resistant Gram-negative bacteria infection. In addition, bacterial resistance to colistin could be transmitted between different species through plasmid-mediated mcr-1 gene transfer. Therefore, rapid screening of colistin-resistant isolates will play a key role in controlling the spread of resistance and improving patient outcomes. We developed a rapid method for the detection of colistin-resistance in Escherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa bacteria based on Raman spectroscopy and hierarchical cluster analysis. Bacteria were incubated with and without colistin using CAMHB as the liquid culture medium. They were then centrifuged and dried on a glass slide. Five Raman spectra of each of the samples were recorded and analyzed by the hierarchical cluster analysis method to determine whether the bacteria were resistant. To evaluate this method, 123 clinical bacterial isolates (42 isolates of E. coli, 41 isolates of A. baumannii and 40 isolates of P. aeruginosa) were tested. The detection sensitivity and specificity were 90.9% and 91.1%, respectively, compared with the reference broth microdilution method. The screening is easy to perform and can be completed in 1.5 h, suggesting that it holds great potential to be an initial screening method in countries and areas where colistin becomes the last resort antibiotic.


Assuntos
Acinetobacter baumannii/isolamento & purificação , Colistina/farmacologia , Farmacorresistência Bacteriana , Escherichia coli/isolamento & purificação , Pseudomonas aeruginosa/isolamento & purificação , Análise Espectral Raman/métodos , Acinetobacter baumannii/efeitos dos fármacos , Antibacterianos/farmacologia , Análise por Conglomerados , Escherichia coli/efeitos dos fármacos , Humanos , Testes de Sensibilidade Microbiana , Pseudomonas aeruginosa/efeitos dos fármacos
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