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1.
J Parasitol ; 106(1): 71-81, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31995717

RESUMO

An unusual coccidian parasite was described previously from the prostate of a male Antechinus flavipes (family: Dasyuridae; common name: yellow-footed antechinus). Morphometrics and a partial nuclear 18S small subunit rDNA (18S rDNA) sequence were used to assign this parasite to the genus Eimeria; it was named Eimeria taggarti. We generated full nuclear 18S rDNA and mitochondrial genome sequences from this parasite and used the newly completed 18S rDNA and mitochondrial cytochrome c oxidase subunit I (COI) sequences to perform a more in-depth phylogenetic analysis. The parasite clustered closely with Choleoeimeria spp. and Acroeimeria spp. infecting herptiles in a well-supported clade that was the sister lineage to the Eimeriidae sensu stricto. The mitochondrial genome of this parasite contained 2 inverted segments compared to mitochondrial genomes from parasites in the Eimeriidae sensu stricto (i.e., Stieda body-possessing coccidia with 4 dizoic sporocysts); this mitochondrial genome arrangement was shared with the only Choleoeimeria species for which sequence data were available publicly. Examination of histological preparations and TEM images uncovered bivalvate sporocysts and otherwise confirmed previously described morphological features of the parasite. Based on our phylogenetic analyses and histological observations, we propose the generic reclassification of E. taggarti to Choleoeimeria taggarti n. comb.


Assuntos
Coccidiose/veterinária , Eimeriidae/genética , Genoma Mitocondrial/genética , Marsupiais/parasitologia , Próstata/parasitologia , Animais , Coccidiose/parasitologia , DNA de Protozoário/química , DNA Ribossômico/química , Eimeriidae/classificação , Eimeriidae/isolamento & purificação , Eimeriidae/ultraestrutura , Complexo IV da Cadeia de Transporte de Elétrons/genética , Masculino , Anotação de Sequência Molecular , Oocistos/ultraestrutura , Filogenia , Reação em Cadeia da Polimerase , RNA de Protozoário/genética , RNA Ribossômico 18S/genética , Alinhamento de Sequência
2.
Rev Bras Parasitol Vet ; 28(4): 592-604, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31800885

RESUMO

Small non-volant mammals (marsupials and small rodents) were captured at three different timepoints from 23 forest fragments across three municipalities (Alta Floresta, Sinop and Cláudia) covering the Amazonian biome of the Mato Grosso State in Midwestern Brazil. The animal tissues (liver and spleen) and blood were screened using molecular tools for the detection of Babesia, Coxiella, Cytauxzoon, Hepatozoon, Theileria, and Anaplasmataceae agents. A total of 230 specimens (78 rodents and 152 marsupials) were trapped. Hepatozoon and Piroplasmorida agents were detected in the common opossums (Didelphis marsupialis). In turn, all samples (blood, liver, or spleen) collected from the small mammals were negative for the genus Coxiella and the family Anaplasmataceae, as detected by polymerase chain reaction (PCR). Phylogenetic analyses inferred from partial sequences of the 18S rRNA gene highlighted the occurrence of new Hepatozoon and Piroplasmorida haplotypes. Future studies determining the role of common opossum (D. marsupialis) in the epidemiological cycles of Hepatozoon and Babesia under natural conditions in the Amazonian biome are necessary.


Assuntos
Marsupiais/parasitologia , RNA Ribossômico 18S/genética , Roedores/parasitologia , Carrapatos/microbiologia , Carrapatos/parasitologia , Anaplasmataceae/genética , Anaplasmataceae/isolamento & purificação , Animais , Babesia/genética , Babesia/isolamento & purificação , Brasil , Coxiella/genética , Coxiella/isolamento & purificação , Filogenia , Inquéritos e Questionários , Theileria/genética , Theileria/isolamento & purificação
3.
Zoolog Sci ; 36(6): 528-538, 2019 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-31833324

RESUMO

Two species of Synactinernus sea anemones were found in Japanese waters. Synactinernus flavus Carlgren, 1918, the only described species of this genus, is rediscovered from off the Goto Islands a century after the original description. Synactinernus flavus was once synonymized with Isactinernus quadrilobatus Carlgren, 1918; however, we show that, based on morphological (including examination of type specimens) and molecular (using nuclear 18S rDNA) evidence, these species are completely different. The other species, Synactinernus churaumi sp. nov., was found off Ishigaki Island and Okinawa Island by a remotely operated vehicle (ROV), and had been kept for 15 years in a tank at the Okinawa Churaumi Aquarium. There are clear differences between these two species; therefore, we describe the second species and revise the diagnosis of Synactinernus.


Assuntos
Antozoários/anatomia & histologia , Antozoários/classificação , Distribuição Animal , Animais , Antozoários/genética , Oceano Pacífico , Filogenia , RNA Ribossômico 16S/genética , RNA Ribossômico 18S/genética , Especificidade da Espécie
4.
Syst Parasitol ; 96(9): 767-776, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31721045

RESUMO

A Henneguya sp., morphologically resembling Henneguya nyongensis Fomena & Bouix, 1996, was isolated from the gills of Peter's elephantnose fish, Gnathonemus petersii Günther, imported from Nigeria. Plasmodia were located between lamellae and within the gill epithelium, often leading to lamellar fusion. Although slightly smaller, the myxospores from these fish were morphologically consistent with H. nyongensis. In valvular view, spores are elongate, pyriform with a rounded posterior and tapering caudal processes. Myxospore bodies are 9.6-12.3 (mean 11.2) µm long and 4.0-4.7 (mean 4.3) µm wide. Polar capsules are pyriform, elongate, 4.5-5.2 (4.7) µm long and 1.3-1.6 (1.4) µm wide, with a characteristic neck-like structure at the apical end. Sequence generated for the 18S small subunit rRNA gene did not directly match any sequences available on GenBank, but demonstrated 91% nucleotide similarity to an unpublished Henneguya sp. infecting Mormyrus kannume Forsskål. Herein, the description of H. nyongensis is supplemented with new data on histopathology, molecular characterisation, and expanded host and geographical range.


Assuntos
Cnidários/classificação , Peixe Elétrico/parasitologia , Doenças dos Peixes/parasitologia , Doenças Parasitárias em Animais/parasitologia , Animais , Cnidários/anatomia & histologia , Cnidários/genética , Doenças dos Peixes/patologia , Brânquias/parasitologia , Brânquias/patologia , Nigéria , Doenças Parasitárias em Animais/patologia , RNA Ribossômico 18S/genética , Especificidade da Espécie
5.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(5): 474-478, 2019 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-31713374

RESUMO

OBJECTIVE: To investigate the prevalence and molecular features of Cryptosporidium in sheep and goats from Anhui Province and neighboring provinces. METHODS: A total 832 and 781 fresh fecal samples were collected from seven large-scale sheep farms and ten large-scale goat farms in Anhui Province and neighboring provinces of Henan, Jiangsu and Shandong. The prevalence and species of Cryptosporidium were investigated in the fecal samples from the sheep and goats in the study areas using nested PCR assay based on the Cryptosporidium-specific SSU rDNA gene, and the subgenotypes of C. parvum and C. ubiquitum were characterized by amplification and sequencing of the 60 kDa glycoprotein (gp60) gene. RESULTS: The overall prevalence of Cryptosporidium was 5.8% (48/832) in sheep and 8.7% (68/781) in goats in Anhui Province and neighboring provinces, respectively. The SSU rDNA gene-based PCR assay identified C. xiaoi and C. ubiquitum in sheep and C. parvum in goats, and subtyping revealed that all C. ubiquitum subgenotypes belonged to XIIa subtype 2 and C. parvum subgenotypes belonged to IIdA19G1. CONCLUSIONS: The identification of zoonotic C. ubiquitum XIIa subtype 2 and C. parvum subtype IIdA19G1 suggests that sheep and goats may serve as a potential source for human Cryptosporidium infections.


Assuntos
Criptosporidiose , Cryptosporidium , Doenças das Cabras , Doenças dos Ovinos , Animais , Criptosporidiose/epidemiologia , Criptosporidiose/parasitologia , Cryptosporidium/classificação , Cryptosporidium/genética , Fezes/parasitologia , Genótipo , Doenças das Cabras/epidemiologia , Doenças das Cabras/parasitologia , Cabras , Humanos , Reação em Cadeia da Polimerase , Prevalência , RNA Ribossômico 18S/genética , Ovinos , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/parasitologia
6.
Parasitol Res ; 118(12): 3241-3252, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31728722

RESUMO

Myxobolus neurofontinalis n. sp. infects the brain and medulla oblongata of brook trout (Salvelinus fontinalis [Mitchill, 1814]) in the New River, western NC. It is the first species of Myxobolus described from the brook trout and resembles another congener (Myxobolus arcticus Pugachev and Khokhlov, 1979) that infects nerve tissue of chars (Salvelinus spp.). The new species differs from M. arcticus and all congeners by myxospore dimensions and by having a mucous envelope and distinctive sutural markings. A phylogenetic analysis of the small subunit rDNA (18S) suggests that the new species shares a recent common ancestor with some isolates identified as M. arcticus and that the new species and its close relatives (except Myxobolus insidiosus Wyatt and Pratt, 1973) comprise a clade of salmonid nerve-infecting myxobolids. The phylogenetic analysis indicates that several isolates of "M. arcticus" (sensu lato) in GenBank are misidentified and distantly related to other isolates taken from the type host (Oncorhynchus nerka [Walbaum, 1792]) and from nearby the type locality (Kamchatka Peninsula, Russia). Serial histological sections of infected brook trout confirmed that myxospores of the new species are intercellular and infect nerve cord and medulla oblongata only. A single infected brook trout showed an inflammatory response characterized by focal lymphocytic infiltrates and eosinophilic granulocytes; however, the remaining 4 brook trout lacked evidence of a histopathological change or demonstrable host response. These results do not support the notion that this infection is pathogenic among brook trout.


Assuntos
Doenças dos Peixes/parasitologia , Bulbo/parasitologia , Myxobolus/classificação , Tecido Nervoso/parasitologia , Truta/parasitologia , Animais , Região dos Apalaches , Doenças dos Peixes/patologia , Myxobolus/genética , Filogenia , RNA Ribossômico 18S/genética , Especificidade da Espécie
7.
Parasitol Res ; 118(12): 3491-3496, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31728723

RESUMO

In this study, 36.8% (28/76) of tissue samples collected from domestic pigs (Sus scrofa) contained sarcocysts, as determined by light microscopy. The organisms were identified as Sarcocystis miescheriana and Sarcocystis suihominis based on their morphological and molecular characteristics. Four genetic markers, i.e., 18S rDNA, 28S rDNA, ITS-1 region (ITS-1), and the mitochondrial COX1 gene (COX1), of the two parasites were sequenced and analyzed, and the 28S rDNA and ITS-1 of S. suihominis obtained from pigs constituted the first records of these markers in GenBank. The sequences of the four loci (18S rDNA, 28S rDNA, ITS-1, and COX1) of S. miescheriana shared high identities with those of S. miescheriana obtained from domestic and/or wild pigs in GenBank, with similarities of 99.6%, 99.6%, 95.9%, and 95.4%, respectively. The 18S rDNA sequences of S. suihominis exhibited 99.4% identity with those of S. suihominis from domestic and wild pigs. The comparison of the newly obtained sequences of the four genetic markers between the two parasites revealed that the interspecific similarities of 18S rDNA, 28S rDNA, ITS-1, and COX1 were 97.7%, 96.6%, 80.3%, and 81.2%, respectively. Therefore, the two species could be better discriminated with ITS-1 and mitochondrial COX1 compared with 18S rDNA or 28S rDNA. The phylogenetic analysis using 28S rDNA indicated that the two Sarcocystis species in domestic pigs had a close relationship.


Assuntos
Sarcocystis/crescimento & desenvolvimento , Sarcocystis/genética , Sarcocistose/veterinária , Doenças dos Suínos/parasitologia , Animais , China , DNA de Protozoário/genética , DNA Ribossômico/genética , Genes Mitocondriais , Filogenia , Reação em Cadeia da Polimerase , RNA Ribossômico 18S/genética , Sarcocystis/classificação , Sarcocystis/isolamento & purificação , Sarcocistose/parasitologia , Sus scrofa/parasitologia , Suínos
8.
Parasitol Res ; 118(12): 3349-3357, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31729574

RESUMO

The genus Chloromyxum (Myxozoa: Myxosporea: Bivalvulida) is defined as having ridged or smooth bivalvular myxospores containing four polar capsules, with/without caudal filaments. Currently containing more than 140 nominal species, this genus is reasonably speciose with myxospores of unique but heterogeneous morphology. Recent phylogenetic studies have demonstrated its polyphyletic nature. During our myxosporean survey of freshwater fish, a new coelozoic myxosporean species, Chloromyxum trilineatum n. sp., was detected in the gall bladder of the pale chub, Zacco platypus (Cypriniformes: Cyprinidae), which originated from central Japan. Spores were subspherical, measuring 8.5-9.1 (8.8) µm in length, 7.6-8.2 (8.0) µm in width, and 6.8-7.8 (7.4) µm in thickness (n = 20). The valvular surface was smooth and three or four distinct ridges ran parallel to the suture line. Four almost equal polar capsules, 2.9-3.8 (3.3) µm in length and 1.6-2.4 (2.0) µm in width, assembled at the apical part of the spores. The partial nucleotide sequence of the 18S ribosomal RNA gene, 2014 bp in length, was closest to that of morphologically distinct Chloromyxum ellipticum, infecting the gall bladder of grass carp (Ctenopharyngodon idella) in China with 96.99% (1673/1725) identity and three insertion/deletion (indel) sites, followed by Chloromyxum legeri, infecting the gall bladder of common carp (Cyprinus carpio) in the Czech Republic with 89.97% (1803/2004) identity and 14 indel sites. Other myxosporean species, including Chloromyxum spp. from the gall bladder or urinary system of freshwater and marine fish, were phylogenetically distant from the present species.


Assuntos
Cyprinidae/parasitologia , Doenças dos Peixes/parasitologia , Vesícula Biliar/parasitologia , Myxozoa/classificação , Doenças Parasitárias em Animais/parasitologia , Animais , Sequência de Bases , China , República Tcheca , DNA Ribossômico/genética , Japão , Myxozoa/genética , Filogenia , RNA Ribossômico 18S/genética
9.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(4): 388-392, 2019 Sep 19.
Artigo em Chinês | MEDLINE | ID: mdl-31612673

RESUMO

OBJECTIVE: To establish a recombinase-aided isothermal amplification (RAA) assay for detection of Cryptosporidium. METHODS: Based on Cryptosporidium-specific 18S rRNA selected as the target gene to be detected, and the primer sequences and fluorescent probes designed using the software Amplfix, and a fluorescent RAA assay was established and optimized. The fluorescent RAA assay was performed to detect 18S RNA target sequence-contained recombinant plasmids at various copies, genomic DNA of Cryptosporidium oocysts at various concentrations, and genomic DNA extracted from various numbers of Cryptosporidium oocysts to assess the sensitivity of the assay, and to detect genomic DNA extracted from Cryptosporidium oocysts, Giardia lamblia cysts, Schistosoma japonicum eggs, Ascaris lumbricoides eggs, Clonorchis sinensis eggs, Salmonella and Shigella to determine the specificity of the assay. RESULTS: A fluorescent RAA assay was successfully established, which was effective to amplify the specific 18S RNA gene fragments of Cryptosporidium within 20 min at 39 ℃. The lowest limits of the fluorescent RAA assay were 102 copies/µL for detection of 18S RNA target sequence-contained recombinant plasmids at various copies, 1 pg/µL for detection of genomic DNA of Cryptosporidium oocysts at various concentrations, and one Cryptosporidium oocyst/µL for detection of genomic DNA extracted from various numbers of Cryptosporidium oocysts, and the fluorescent RAA assay was all negative for detection of genomic DNA from G. lamblia cysts, S. japonicum eggs, A. lumbricoides eggs, C. sinensis eggs, Salmonella and Shigella. CONCLUSIONS: A novel fluorescent RAA assay is successfully established, which is simple, rapid, sensitive and specific to detect genomic DNA of Cryptosporidium oocysts.


Assuntos
Cryptosporidium , Técnicas de Amplificação de Ácido Nucleico/métodos , Técnicas de Amplificação de Ácido Nucleico/normas , Cryptosporidium/genética , DNA de Protozoário/genética , Limite de Detecção , Oocistos , RNA Ribossômico 18S/genética , Recombinases/metabolismo , Sensibilidade e Especificidade
10.
J Fish Dis ; 42(12): 1745-1760, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31637741

RESUMO

To identify the pathogens causing saprolegniosis among farmed fish in Nova Scotia, 172 infected tissues and 23 water samples were collected from six species of teleosts: Atlantic salmon (Salmo salar), brown trout (Salmo trutta), Arctic charr (Salvelinus alpinus), brook trout (Salvelinus fontinalis), striped bass (Morone saxatilis) and rainbow trout (Oncorhynchus mykiss) at nine facilities over a 600 km range. Following laboratory culture, 132 isolates were recovered. Six species of oomycetes were identified from analysis of the internal transcribed spacer (ITS) sequence of the nrDNA: Saprolegnia parasitica, Saprolegnia ferax, Saprolegnia diclina, Saprolegnia aenigmatica, Saprolegnia torulosa, Saprolegnia sp. and Pythiopsis cymosa. Further phylogenetic analyses of the ITS and cytochrome c oxidase subunit 1 (Cox1) regions revealed four strains of Saprolegnia parasitica (named here as S1, S2, S3 and S4), of which S1 and S2 were common (37% and 42% of the isolates), and two strains of S. ferax. Among S. parasitica, S2 and S3 are more closely related to each other than to S1 based on the phylogenetic analyses and predicted RNA secondary structure of the ITS region. Sexual structures with a similar morphology were formed by S1 and S3 in vitro, but were not formed by S2.


Assuntos
Doenças dos Peixes/parasitologia , Filogenia , Saprolegnia/classificação , Animais , Bass/parasitologia , DNA Espaçador Ribossômico/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , Pesqueiros , Nova Escócia , Conformação de Ácido Nucleico , Oncorhynchus mykiss/parasitologia , RNA Ribossômico 18S/genética , Salmo salar/parasitologia , Truta/parasitologia
11.
Ann Parasitol ; 65(3): 237-243, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31599539

RESUMO

Rabbits are commonly reared by households and farmers in Nigeria as a source of meat, but there is no information available on Cryptosporidium genotypes occurring in rabbits in Nigeria. Fecal samples were collected from 107 rabbits and examined by modified Ziehl-Neelsen technique for the presence of Cryptosporidium oocysts. An infection rate of 3.7% (4/107) was obtained and all microscopy-positive samples were genotyped and subtyped to determine the circulating Cryptosporidium species using sequence analysis of the 18S rRNA gene and 60-kDa glycoprotein (gp60) gene, respectively. All the four microscopy-positive samples were identified as C. parvum by 18S rRNA gene. However, analysis of the gp60 gene revealed the presence of C. parvum subtype IIc, which is commonly found in humans in two isolates. These findings indicate natural infection of rabbits with C. parvum and underscore the need to investigate the probable role of animal hosts in the epidemiology of Cryptosporidium infection. This is the first report on genetic characterization of Cryptosporidium infecting rabbits in Nigeria.


Assuntos
Criptosporidiose , Cryptosporidium parvum , Doenças Parasitárias em Animais , Animais , Criptosporidiose/diagnóstico , Criptosporidiose/epidemiologia , Criptosporidiose/parasitologia , Cryptosporidium parvum/genética , Fezes/parasitologia , Genótipo , Humanos , Nigéria/epidemiologia , Doenças Parasitárias em Animais/diagnóstico , Doenças Parasitárias em Animais/epidemiologia , Doenças Parasitárias em Animais/parasitologia , Reação em Cadeia da Polimerase , RNA Ribossômico 18S/genética , Coelhos , Análise de Sequência de DNA , Sialoglicoproteínas/genética
12.
Eur J Protistol ; 71: 125634, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31585231

RESUMO

Vannella samoroda n. sp. (Amoebozoa, Vannellida) was isolated from the mouth of the Malaya Samoroda river flowing into Elton, the largest European hypersaline lake (Russia). Among all rivers of the area, it has the highest salt content (ca. 110‰). Amoebae maintained in seawater medium with ca. 77‰ salts concentration had a set of morphological characters typical of Vannella spp.: rounded, fan-shaped, or spatulate locomotive form, floating form with bent, blunt-ended hyaline pseudopodia, and a cell coat consisting of regularly packed palisade elements and scarce simple filaments. Phylogenetic analyses based on SSU rRNA and cytochrome C oxidase subunit 1 genes show that the amoeba is most closely related to Vannella ebro Smirnov, 2001, but represents a distinct species. The clade of V. ebro and V. samoroda branches among marine species of Vannella. The studied species is the first member of the genus Vannella from a continental saline habitat described using molecular data. Interestingly, it has a broad range of salinity tolerance: cells reproduce above 18‰, while survival of a few cells regularly occurs even in highly diluted Prescott and James medium. The normal culture restores itself when PJ medium is substituted with 77‰ seawater medium even after months of experimental incubation.


Assuntos
Amebozoários/classificação , Filogenia , Água do Mar/parasitologia , Amebozoários/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , RNA Ribossômico 18S/genética , Federação Russa , Especificidade da Espécie
13.
Eur J Protistol ; 71: 125636, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31585232

RESUMO

The parasitic dinoflagellate Syltodinium listii was investigated from the open waters of the English Channel, southern North Sea and the NW Mediterranean Sea. Syltodinium listii has been unreported since its original description in the North Sea. Cells of S. listii were able to infect copepod eggs of different species, and even nauplii, and after each infection formed up to 32 cells embedded in a mucous envelope. Infection of the same host by more than one dinoflagellate was frequent; although overall, the progeny were reduced in number. Molecular phylogeny based on the small subunit ribosomal RNA (SSU rRNA) gene revealed that S. listii clusters with a group of environmental sequences from the cold North Atlantic region as a sister group of Gymnodinium aureolum. The large subunit ribosomal RNA (LSU rRNA) gene sequences of S. listii from the English Channel and cf. Gyrodinium undulans from the Mediterranean Sea were identical. Thus, we propose Syltodinium undulans comb. nov. for Gyrodinium undulans. The first internal transcribed spacer (ITS) and complete SSU rRNA gene sequences of Dissodinium pseudolunula are provided. The parasitic species of Chytriodinium, Dissodinium and Syltodinium cluster together within the family Chytriodiniaceae, including the free-living species Gymnodinium aureolum, G. corollarium and G. plasticum.


Assuntos
Dinoflagelados/classificação , Filogenia , DNA de Protozoário/genética , Dinoflagelados/genética , Mar Mediterrâneo , RNA Ribossômico 18S/genética , Especificidade da Espécie
14.
Rev Bras Parasitol Vet ; 28(4): 563-568, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31576972

RESUMO

Chiggers are ectoparasites of vertebrates and may cause trombiculiasis or transmit pathogens to their hosts. Specimens collected from rodents and marsupials were morphologically identified as Herpetacarus hertigi, Eutrombicula tinami, Kymocta sp., Quadraseta brasiliensis, Quadraseta falconensis, Quadraseta flochi, Quadraseta mackenziei, Quadraseta pazca, Quadraseta trapezoides, Quadraseta sp., Serratacarus sp., and Trombewingia bakeri. These mites were submitted individually to molecular analyses for the detection of bacteria of the genus Coxiella, Hepatozoon and Rickettsia. Samples were positive to Rickettsia only. Obtained sequences for the gltA (350 pb) and ompA (488 pb) genes were identical to "Candidatus Rickettsia colombianensi", a species previously detected in ticks. In addition, molecular identification of mites based on 18S rDNA sequences are provided for H. hertigi, Kymocta sp., Q. brasiliensis, Q. pazca, Q. trapezoides, Quadraseta sp., and T. bakeri for the first time. This is the first report of the detection of a Rickettsia sp. in chigger mites collected on rodents in Brazil.


Assuntos
Marsupiais/parasitologia , Infestações por Ácaros/veterinária , Rickettsia/genética , Roedores/parasitologia , Trombiculidae/microbiologia , Animais , Reação em Cadeia da Polimerase , RNA Bacteriano/genética , RNA Ribossômico 18S/genética , Rickettsia/isolamento & purificação
15.
Eur J Protistol ; 71: 125641, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31639568

RESUMO

A new hypotrichous ciliate, Oxytricha seokmoensis sp. nov., was discovered in a soil from a forest in South Korea and described based on the observations of living and stained specimens. In addition, phylogenetic analyses were performed using the small subunit ribosomal RNA (18S rRNA) gene sequence. Morphologically, the new species is similar to the O. granulifera-complex in terms of ciliary structure and arrangement of cortical granules, but dorsal kineties 3 and 4 (not completely separated vs. separated) and macronuclear nodules in the cyst (separated vs. fused) differ. Oxytricha seokmoensis is most similar to O. pulvillus, but can be distinguished by the number of adoral membranelles (30-40 vs. 23-27), contractile vacuole (present vs. absent), number of left (27-37 vs. 17-25) and right (27-35 vs. 18-23) marginal cirri, and lepidosomes on the cyst surface (present vs. absent). In a phylogenetic tree, O. seokmoensis is distinctly separated from the O. granulifera clade, but is sister to the Paroxytricha clade. In addition, O. seokmoensis and P. longigranulosa have the smallest genetic difference (d = 0.015, 23 of 1579 nt difference). This close relationship is supported by incomplete dorsal kinety 3 fragmentation and separated macronuclear nodules in resting cysts.


Assuntos
Oxytricha/classificação , Filogenia , Oxytricha/citologia , Oxytricha/genética , Oxytricha/crescimento & desenvolvimento , RNA Ribossômico 18S/genética , Especificidade da Espécie
16.
Eur J Protistol ; 71: 125630, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31557698

RESUMO

Two brackish water amoebae have been isolated and studied from the benthic biotopes of the Chupa Inlet (Kandalaksha Bay, northwestern Russia). Both strains can be identified as new species of the genus Paramoeba (Amoebozoa, Dactylopodida, Paramoebidae) based on light microscopical characters, structure of microscales on the cell surface and molecular evidence based on the analyses of two genes, nuclear SSU rRNA and mitochondrial cytochrome c oxidase subunit 1 (COI). Paramoeba aparasomata n. sp. is of particular interest because this amoeba is permanently lacking a symbiotic Perkinsela-like organism (PLO) present in other species of Paramoeba and Neoparamoeba. The results obtained show that scaly dactylopodial amoebae lacking PLO are not necessarily members of Korotnevella. In particular, we suggest that Korotnevella nivo Smirnov, 1997, with microscales very similar to those of Paramoeba eilhardi and the species studied here in structure, may be in fact a member of Paramoeba. Molecular data on K. nivo have to be obtained and analysed to test this hypothesis. Based on our new results we emend the diagnosis of the genus Paramoeba to make it more fit to the current phylogenetic conception.


Assuntos
Amebozoários/classificação , Amebozoários/citologia , Amebozoários/genética , Amebozoários/parasitologia , Complexo IV da Cadeia de Transporte de Elétrons/genética , Kinetoplastida/fisiologia , RNA Ribossômico 18S/genética , Federação Russa , Águas Salinas , Especificidade da Espécie , Simbiose
17.
Rev Soc Bras Med Trop ; 52: e20190171, 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31508782

RESUMO

INTRODUCTION: Biomphalaria glabrata is considered to be responsible for the incidence of schistosomiasis in Brazil. Therefore, surveillance of areas where schistosomiasis is prevalent is fundamental for public health planning. This study was aimed to evaluate B. glabrata populations in water bodies of the city of Salvador, determine their distribution, estimate the prevalence of Schistosoma mansoni infections, characterize shed cercariae, and identify transmission foci. METHODS: Malacological surveys were carried out in 17 water collections from Salvador. Snail species were identified based on shell and mantle characteristics. Snails were evaluated for S. mansoni infection by exposure to light and via real time polymerase chain reaction (qPCR) using S. mansoni-18S rRNA subunit specific primers. RESULTS: 1,403 B. glabrata were collected. Classical cercarial shedding indicated that 5 snails (0.4%) were positive for S. mansoni. A higher prevalence of infections was found in Horta de Saramandaia (5.5%) and Lagoa do IAT (1.9%). Non-Schistosoma larvae, such as Xiphidiocercaria, Strigeidae, Spirorchiidae and Clinostomidae, were observed in 3.2% of the snails. S. mansoni DNA was detected in 6.2% snails via qPCR. CONCLUSIONS: B. glabrata is widely distributed in Salvador, as indicated by 7 water collections associated with a risk of schistosomiasis transmission. To our knowledge, this is the first study to identify B. glabrata eliminating cercariae of Clinostomidae, Strigeidae, and Spirorchiidae in Salvador. We propose that qPCR may be employed in combination with classical cercarial shedding. Estimating S. mansoni prevalence in snails by only considering the results of light exposure method classical into account may underestimate the problem.


Assuntos
Biomphalaria/parasitologia , Vetores de Doenças , Schistosoma mansoni/genética , Animais , Humanos , RNA Ribossômico 18S/genética , Reação em Cadeia da Polimerase em Tempo Real , Schistosoma mansoni/isolamento & purificação , Esquistossomose mansoni/transmissão , População Urbana
18.
Fa Yi Xue Za Zhi ; 35(4): 444-447, 2019 Aug.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-31532155

RESUMO

Abstract: Objective To detect the diatom population diversity in Dianchi by constructing a 18S rDNA clone library. Methods DNA from diatoms in 6 water samples of Dianchi was amplified with diatom 18S rDNA specific primer.The 18S rDNA clone library was constructed, and clones were randomly selected for sequence. Sequence alignment was performed by BLAST. The diatom population distribution in Dianchi was analyzed and the phylogenetic tree of diatom 18S rDNA in Dianchi waters was established with the MEGA v7.0.14 software. Results Two hundred and forty clones were sequenced, with 167 diatom sequences obtained, including 11 diatom species such as Stephanodiscus, Diatoma, and Melosira. There were certain differences in diatom population distribution among the 6 samples. Conclusion The population distribution of diatom species in Dianchi shows unique features and the sequence analysis of diatom 18S rDNA has a certain reference value to the inference of forensic drowning sites.


Assuntos
Diatomáceas/classificação , Filogenia , RNA Ribossômico 18S/genética , China , DNA Ribossômico/genética , Afogamento , Ciências Forenses , Humanos
19.
Korean J Parasitol ; 57(4): 423-427, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31533410

RESUMO

Coenurosis is an important zoonotic helminthic disease caused by the larval stage of the tapeworm Taenia multiceps. This parasite typically infects the brain of the intermediate hosts, including sheep, goat, cattle and even humans. We report a case of T. multiceps infection in a yak confirmed by clinical symptoms, morphological characteristics, and molecular and phylogenetic analyses. The coenurus was thin-walled, whitish, and spherical in shape with a diameter of 10 cm. The parasite species was identified as T. multiceps by PCR amplification and sequencing of the 18S rRNA, cox1 and nad1 genes. Three gene sequences all showed high homology (all above 97%) with the reference sequences from different hosts. Moreover, phylogenetic reconstructions with the 3 published Taenia gene sequences confirmed that the Qinghai yak isolate was closely related to T. multiceps. Although there are advanced diagnosis and treatment methods for coenurosis, early infection is difficult to diagnose. Importantly, the findings of yak infection case should not be ignored due to its zoonotic potential.


Assuntos
Doenças dos Bovinos/parasitologia , Neurocisticercose/veterinária , Taenia/genética , Animais , Bovinos , Ciclo-Oxigenase 1/genética , Eletroforese em Gel de Ágar/veterinária , Masculino , NAD/genética , Neurocisticercose/parasitologia , Filogenia , Reação em Cadeia da Polimerase/veterinária , RNA Ribossômico 18S/genética , Alinhamento de Sequência/veterinária , Análise de Sequência de DNA/veterinária , Taenia/classificação , Taenia/isolamento & purificação , Tibet
20.
Korean J Parasitol ; 57(4): 439-444, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31533413

RESUMO

Since Kudoa septempuntata was identified as a causative agent of food poisoning associated with raw olive flounder Paralichthys olivaceus, interest and concern regarding the parasite have increased. However, there have been no investigations or reports of other Kudoa species infecting the fish (except for K. paralichthys, which infects the brain) in Korea. We found cysts filled with myxospores of Kudoa species in muscles of cultured olive flounder specimens and identified these to the species level. Mature spores were quadrate, measuring 8.7±0.5 µm in length, 9.2±0.4 µm in thickness, and 12.9±0.6 µm in width. The spores containing 4 polar capsules had a length of 2.1±0.2 µm and a width of 1.8±0.3 µm. The partial 18S and 28S rDNA of isolates showed 99-100% similarities with K. ogawai. Using these morphological and molecular analyses, the species was identified as K. ogawai. This study is the first report of K. ogawai infection in cultured olive flounder in Korea.


Assuntos
Doenças dos Peixes/parasitologia , Linguado/parasitologia , Myxozoa/isolamento & purificação , Doenças Parasitárias em Animais/parasitologia , Animais , Sequência de Bases , DNA Ribossômico/química , Pesqueiros , Músculos/parasitologia , Myxozoa/genética , RNA Ribossômico 18S/genética , RNA Ribossômico 28S/genética , República da Coreia
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