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1.
Lasers Med Sci ; 35(1): 157-164, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31168678

RESUMO

The aim of the present study was to evaluate the in vivo response of different wavelengths (red and near-infrared) of light-emitting diode (LED) on full-thickness skin grafts (FTSG) in rats. Thirty rats were randomly allocated into three experimental groups: control group (C); red LED treated group (R); and near-infrared LED group (NIR). Skin grafts were irradiated daily for ten consecutive days, starting immediately after the surgery using a red (630 nm) or near-infrared (850 nm) LED. The results showed that the red wavelength LED significantly enhanced the skin graft score in relation to the NIR group and increased transforming growth factor beta (TGF-ß) protein expression and density of collagen fibers compared with the other experimental groups. These results suggest that the red wavelength LED was efficient to improve the dermo-epidermal junction and modulate the expression proteins related to tissue repair.


Assuntos
Raios Infravermelhos/uso terapêutico , Fototerapia/métodos , Transplante de Pele , Animais , Colágeno/metabolismo , Epiderme/metabolismo , Epiderme/efeitos da radiação , Regulação da Expressão Gênica/efeitos da radiação , Masculino , Ratos , Fator de Crescimento Transformador beta/metabolismo , Cicatrização/fisiologia , Cicatrização/efeitos da radiação
2.
J Strength Cond Res ; 34(1): 235-248, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30113919

RESUMO

Nunes, RFH, Cidral-Filho, FJ, Flores, LJF, Nakamura, FY, Rodriguez, HFM, Bobinski, F, De Sousa, A, Petronilho, F, Danielski, LG, Martins, MM, Martins, DF, and Guglielmo, LGA. Effects of far-infrared emitting ceramic materials on recovery during 2-week preseason of elite futsal players. J Strength Cond Res 34(1): 235-248, 2020-We investigated the effects of far-infrared emitting ceramic materials (cFIR) during overnight sleep on neuromuscular, biochemical and perceptual markers in futsal players. Twenty athletes performed a 2-week preseason training program and during sleep wore bioceramic (BIO; n = 10) or placebo pants (PL; n = 10). Performance (countermovement jump [CMJ]; squat jump [SJ]; sprints 5, 10, and 15-m) and biochemical markers (tumor necrosis factor alpha-TNF-α, interleukin 10-IL-10, thiobarbituric acid-reactive species [TBARS], carbonyl, superoxide dismutase [SOD], catalase [CAT]) were obtained at baseline and after the 1st and 2nd week of training. Delayed-onset muscle soreness (DOMS) and training strain were monitored throughout. Changes in ΔCMJ and ΔSJ were possibly (60/36/4 [week-1]) and likely (76/22/2 [week-2]) higher in BIO. Both groups were faster in 5-m sprint in week 2 compared with baseline (p = 0.015), furthermore, BIO was likely faster in 10-m sprint (3/25/72 [week 1]). Significant group × time interaction in %ΔTNF-α were observed (p = 0.024 [week-1]; p = 0.021 [week-2]) with values possibly (53/44/3 [week 1]) and likely (80/19/1 [week 2]) higher in BIO. The %ΔIL-10 decreased across weeks compared with baseline (p = 0.019 [week-1]; p = 0.026 [week-2]), showing values likely higher in BIO (81/16/3 [week-1]; 80/17/3 [week-2]). Significant weekly increases in %ΔTBARS (p = 0.001 [week-1]; p = 0.011 [week-2]) and %ΔCarbonyl (p = 0.002 [week-1]; p < 0.001 [week-2]) were observed compared with baseline, showing likely (91/5/4 [week-1]) and possibly (68/30/2 [week-2]) higher changes in BIO. Significant weekly decreases in %ΔSOD were observed compared with baseline (p = 0.046 [week 1]; p = 0.011 [week-2]), and between week 2 and week 1 (p = 0.021), in addition to significant decreases in %ΔCAT compared with baseline (p = 0.070 [week 1]; p = 0.012 [week 2]). Training strain (p = 0.021; very -likely [0/2/98]; week 1) and DOMS was lower in BIO (likely; 7 sessions) with differences over time (p = 0.001). The results suggest that the daily use of cFIR clothing could facilitate recovery, especially on perceptual markers during the early phases of an intensive training period.


Assuntos
Desempenho Atlético/fisiologia , Raios Infravermelhos/uso terapêutico , Condicionamento Físico Humano/fisiologia , Futebol/fisiologia , Adulto , Biomarcadores/sangue , Catalase/sangue , Cerâmica , Vestuário , Método Duplo-Cego , Teste de Esforço , Humanos , Interleucina-10/sangue , Movimento , Força Muscular/fisiologia , Músculo Esquelético/fisiopatologia , Mialgia/etiologia , Mialgia/terapia , Condicionamento Físico Humano/efeitos adversos , Recuperação de Função Fisiológica , Corrida , Sono , Superóxido Dismutase/sangue , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo , Fator de Necrose Tumoral alfa/sangue , Adulto Jovem
3.
Artif Cells Nanomed Biotechnol ; 47(1): 4293-4304, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31810396

RESUMO

Instability of silk fibroin nanoparticles (SFNPs) in physiologic condition hinders its application as drug delivery vehicle. Herein, indocyanine green (ICG) loaded silk fibroin nanoparticles (ICG-SFNPs) was firstly prepared and then crosslinked by proanthocyanidins to obtain the stable ICG-CSFNPs for killing the residual tumour niche under near infra-red irradiation (NIR) after surgery. The particle size and zeta potentials of ICG-CSFNPs was 120.1 nm and -40.4 mV, respectively. Moreover, ICG-CSFNPs exhibited good stability of particle size in the physiological medium. Meanwhile, the stable photothermal properties of ICG-CSFNPs were not compromised even after several cycles of NIR. Few of the ICG-CSFNPs were phagocytized by RAW264.7 macrophage in vitro, while they were easily internalized by C6 glioma cells, resulting in their significant toxicity on tumour cells after NIR. The pharmacokinetic study showed that ICG-CSFNPs had a longer blood circulation time than ICG-SFNPs, making them more distribution in glioma after intravenous administration in vivo. Meanwhile, the pharmacological study showed the more effective inhibition of tumour growth was exhibited by ICG-CSFNPs in C6 glioma-bearing mice after NIR. Overall, the cross-linked nanoparticles of silk fibroin may be a promising vehicle of ICG for photothermal therapy of glioma after surgical resection.


Assuntos
Fibroínas/química , Glioma/terapia , Verde de Indocianina/química , Verde de Indocianina/uso terapêutico , Nanopartículas/química , Fototerapia , Proantocianidinas/química , Animais , Linhagem Celular Tumoral , Portadores de Fármacos/química , Liberação Controlada de Fármacos , Glioma/diagnóstico por imagem , Glioma/patologia , Verde de Indocianina/farmacocinética , Raios Infravermelhos/uso terapêutico , Masculino , Camundongos , Imagem Óptica , Ratos , Distribuição Tecidual
4.
Obesity (Silver Spring) ; 27(12): 1932-1949, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31691547

RESUMO

OBJECTIVE: Brown adipose tissue (BAT) is a thermogenic tissue with potential as a therapeutic target in the treatment of obesity and related metabolic disorders. The most used technique for quantifying human BAT activity is the measurement of 18 F-fluorodeoxyglucose uptake via a positron emission tomography/computed tomography scan following exposure to cold. However, several studies have indicated the measurement of the supraclavicular skin temperature (SST) by infrared thermography (IRT) to be a less invasive alternative. This work reviews the state of the art of this latter method as a means of determining BAT activity in humans. METHODS: The data sources for this review were PubMed, Web of Science, and EBSCOhost (SPORTdiscus), and eligible studies were those conducted in humans. RESULTS: In most studies in which participants were first cooled, an increase in IRT-measured SST was noted. However, only 5 of 24 such studies also involved a nuclear technique that confirmed increased activity in BAT, and only 2 took into account the thickness of the fat layer when measuring SST by IRT. CONCLUSIONS: More work is needed to understand the involvement of tissues other than BAT in determining IRT-measured SST; at present, IRT cannot determine whether any increase in SST is due to increased BAT activity.


Assuntos
Tecido Adiposo Marrom/metabolismo , Raios Infravermelhos/uso terapêutico , Temperatura Cutânea/fisiologia , Termografia/métodos , Adolescente , Adulto , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos
5.
Medicine (Baltimore) ; 98(38): e17193, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31567965

RESUMO

BACKGROUND: Sleep bruxism is a masticatory muscle activity characterized as rhythmic (phasic) or nonrhythmic (tonic). In children and adolescents, etiological factors, such as breathing pattern and sleep quality, have recently been addressed in studies investigating sleep bruxism. New therapies for adults, such as botulinum toxin, have been investigated, but such techniques are not applicable for individuals in the growth and development phase. METHODS: The participants will be 76 children, which will be randomly allocated to a control group, that is group 1, absence of bruxism; group 2, children with bruxism treated with infrared light-emitting diode (LED); and group 3, bruxism treated with occlusal splint. All participants will be submitted to a clinical evaluation to evaluate muscle activity and salivary biomarkers, before and after treatments. Muscle activity will be verified by electromyography of muscles mastication, masseter and temporal, and salivary biomarkers observed will be cortisol and dopamine levels. DISCUSSION: Photobiomodulation therapy has piqued the interest of researchers, as this noninvasive method has demonstrated positive results in problems related to muscle tissues. This document describes the protocol for a proposed study to evaluate morphological and psychosocial aspects in children and adolescents with awake bruxism and their responses to photobiomodulation therapy with infrared LED. CLINICAL TRIALS:.


Assuntos
Terapia com Luz de Baixa Intensidade/métodos , Bruxismo do Sono/radioterapia , Criança , Protocolos Clínicos , Humanos , Raios Infravermelhos/uso terapêutico , Músculos da Mastigação/fisiopatologia , Músculos da Mastigação/efeitos da radiação , Placas Oclusais , Bruxismo do Sono/fisiopatologia , Bruxismo do Sono/terapia
6.
Photobiomodul Photomed Laser Surg ; 37(10): 651-656, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31647774

RESUMO

Background: Transcranial photobiomodulation (t-PBM) consists in the delivery of near-infrared light (NIR) to the scalp, directed to cortical areas of the brain. NIR t-PBM recently emerged as a potential therapy for depression, although safety of repeated treatments has not been adequately explored. Objective: This study assessed incidence of side effects, including weight and blood pressure changes, during repeated sessions of NIR t-PBM using a light-emitting diode source. Methods: We performed a secondary analysis of a double-blind clinical trial on t-PBM for major depressive disorder. Eighteen individuals received NIR t-PBM (n = 9) or sham (n = 9) twice weekly for 8 weeks. Side effects were assessed using the Systematic Assessment for Treatment-Emergent Effects-Specific Inquiry. In 14 individuals (nNIR = 6 vs. nsham = 8), body weight and systemic blood pressure were recorded at baseline and end-point. Results: More subjects in the NIR t-PBM group experienced side effects compared to sham, but only a trend for statistical significance was observed (χ2 = 3.60; df = 1; p = 0.058). The rate of side effects described by participants as "severe" in intensity was low and similar between the treatment groups (χ2 = 0.4; df = 1; p = 0.53), with no serious adverse events. Most side effects resolved during the study and treatment interruption were not required. Changes in weight and systolic blood pressure across groups were neither significant nor approached significance. In the NIR t-PBM group, diastolic blood pressure increased and reached statistical-however not clinical-significance (5.67 ± 7.26 vs. -6.13 ± 6.88; z = -2.40, p = 0.016). Conclusions: This small-sample, exploratory study indicates repeated sessions of NIR t-PBM might be associated with treatment-emergent side effects. The systemic metabolic and hemodynamic profile of repeated t-PBM appeared benign. Future studies with larger samples and longer follow-up are needed to more accurately determine the side-effect profile and safety of NIR t-PBM.


Assuntos
Peso Corporal , Transtorno Depressivo Maior/terapia , Hipertensão/etiologia , Raios Infravermelhos/efeitos adversos , Terapia com Luz de Baixa Intensidade/efeitos adversos , Adulto , Determinação da Pressão Arterial , Boston , Encéfalo/efeitos da radiação , Distribuição de Qui-Quadrado , Transtorno Depressivo Maior/diagnóstico , Método Duplo-Cego , Feminino , Hospitais Gerais , Humanos , Hipertensão/epidemiologia , Hipertensão/fisiopatologia , Raios Infravermelhos/uso terapêutico , Terapia com Luz de Baixa Intensidade/métodos , Masculino , Pessoa de Meia-Idade , Gravidez , Índice de Gravidade de Doença , Estatísticas não Paramétricas
7.
Photobiomodul Photomed Laser Surg ; 37(10): 644-650, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31647775

RESUMO

Objective: Our aim was to test the anxiolytic effect of transcranial photobiomodulation (t-PBM) with near-infrared light (NIR) in subjects suffering from generalized anxiety disorder (GAD). Background: t-PBM with NIR is an experimental, noninvasive treatment for mood and anxiety disorders. Preliminary evidence indicates a potential anxiolytic effect of transcranial NIR. Methods: Fifteen subjects suffering from GAD were recruited in an open-label 8-week study. Each participant self-administered t-PBM daily, for 20 min (continuous wave; 830 nm peak wavelength; average irradiance 30 mW/cm2; average fluence 36 J/cm2; total energy delivered per session 2.9 kJ: total output power 2.4 W) broadly on the forehead (total area 80 cm2) with an LED-cluster headband (Cerebral Sciences). Outcome measures were the reduction in total scores of the Hamilton Anxiety Scale (SIGH-A), the Clinical Global Impressions-Severity (CGI-S) subscale and the Pittsburgh Sleep Quality Index (PSQI) subscales from baseline to last observation carried forward. Results: Of the 15 recruited subjects (mean age 30 ± 14 years; 67% women), 12 (80%) completed the open trial. Results show a significant reduction in the total scores of SIGH-A (from 17.27 ± 4.89 to 8.47 ± 4.87; p < 0.001; Cohen's d effect size = 1.47), in the CGI-S subscale (from 4.53 ± 0.52 to 2.87 ± 0.83; p < 0.001; Cohen's d effect size = 2.04), as well as significant improvements in sleep at the PSQI. t-PBM was well tolerated with no serious adverse events. Conclusions: Based on our pilot study, t-PBM with NIR is a promising alternative treatment for GAD. Larger, randomized, double-blind, sham-controlled studies are needed.


Assuntos
Transtornos de Ansiedade/diagnóstico , Transtornos de Ansiedade/terapia , Encéfalo/efeitos da radiação , Raios Infravermelhos/uso terapêutico , Segurança do Paciente , Adolescente , Adulto , Fatores Etários , Feminino , Seguimentos , Humanos , Terapia com Luz de Baixa Intensidade/métodos , Masculino , Projetos Piloto , Estudos Prospectivos , Medição de Risco , Índice de Gravidade de Doença , Fatores Sexuais , Crânio/efeitos da radiação , Resultado do Tratamento , Adulto Jovem
8.
Artigo em Russo | MEDLINE | ID: mdl-31626157

RESUMO

AIM: To study the possibility and feasibility of using terahertz-modulated infrared radiation in the treatment of patients with gonarthrosis, including those with concomitant chronic lower limb venous insufficiency and to develop a specific treatment procedure. SUBJECTS AND METHODS: A total of 92 patients were examined and treated. The patients matched for age, gender, and main clinical manifestations were divided into a study group and a control one. All the patients received disease-modifying anti-rheumatic drugs, therapeutic exercises, including those in a swimming pool. In addition to the basic treatment, the patients in the study group had terahertz-modulated infrared radiotherapy. Before and after treatment, all the patients underwent a physical examination; their complaints were studied; pain severity was assessed by a visual analog scale (VAS) for pain; lower limb temperature was measured by infrared beam thermography; and microcirculation in the affected knee joint area was analyzed by laser Doppler flowmetry. RESULTS: Analysis of posttreatment changes in clinical manifestations in the study group revealed a significant reversion of clinical manifestations, such as knee joint pain occurring at the end of the day or in the first half of the night (from 42.6 to 15.3%), as well as pain occurring after mechanical load and reducing at rest (from 72.3 to 25.5%). The changes in mean VAS scores in the study group (from 6.1±0.7 to 2.1±0.6) were significantly pronounced (p<0.01) compared with those in the controls (from 6.6±0.7 to 4.3±0.4). Infrared beam thermography established that the study group had a decrease in the thermal asymmetry value between varicose veins and adjacent tissues, indicating a hemodynamic improvement in the lower extremity vessels (by 1.57±0.07 °C in the study group (p<0.05) versus 0.91±0.04 °C in the control group (p<0.05). The ongoing package of rehabilitation measures assisted in reducing the temperature difference between the affected vein area and adjacent tissues. CONCLUSION: The incorporation of terahertz-modulated infrared radiation into a therapeutic complex was found to have a positive impact on the clinical symptoms of the disease, the state of local hemodynamics and microcirculation, which substantially enhances the efficiency of therapy.


Assuntos
Osteoartrite do Joelho/complicações , Osteoartrite do Joelho/radioterapia , Insuficiência Venosa/complicações , Estudos de Viabilidade , Feminino , Humanos , Raios Infravermelhos/uso terapêutico , Extremidade Inferior/irrigação sanguínea , Masculino , Osteoartrite do Joelho/fisiopatologia , Resultado do Tratamento , Insuficiência Venosa/fisiopatologia
9.
Nat Commun ; 10(1): 4490, 2019 10 03.
Artigo em Inglês | MEDLINE | ID: mdl-31582736

RESUMO

The application of photothermal therapy to treat bacterial infections remains a challenge, as the high temperatures required for bacterial elimination can damage healthy tissues. Here, we develop an exogenous antibacterial agent consisting of zinc-doped Prussian blue (ZnPB) that kills methicillin-resistant Staphylococcus aureus in vitro and in a rat model of cutaneous wound infection. Local heat triggered by the photothermal effect accelerates the release and penetration of ions into the bacteria, resulting in alteration of intracellular metabolic pathways and bacterial killing without systemic toxicity. ZnPB treatment leads to the upregulation of genes involved in tissue remodeling, promotes collagen deposition and enhances wound repair. The efficient photothermal conversion of ZnPB allows the use of relatively few doses and low laser flux, making the platform a potential alternative to current antibiotic therapies against bacterial wound infections.


Assuntos
Antibacterianos/administração & dosagem , Terapia a Laser , Estruturas Metalorgânicas/administração & dosagem , Infecções Estafilocócicas/terapia , Infecção dos Ferimentos/terapia , Administração Cutânea , Animais , Antibacterianos/química , Terapia Combinada/métodos , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Ferrocianetos/administração & dosagem , Ferrocianetos/química , Humanos , Raios Infravermelhos/uso terapêutico , Masculino , Estruturas Metalorgânicas/química , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Camundongos , Testes de Sensibilidade Microbiana , Células NIH 3T3 , Nanopartículas/administração & dosagem , Nanopartículas/química , Ratos , Infecções Estafilocócicas/microbiologia , Resultado do Tratamento , Cicatrização/efeitos dos fármacos , Cicatrização/efeitos da radiação , Infecção dos Ferimentos/microbiologia , Zinco/administração & dosagem , Zinco/química
10.
Nat Commun ; 10(1): 4418, 2019 09 27.
Artigo em Inglês | MEDLINE | ID: mdl-31562357

RESUMO

Smart drug delivery systems (SDDSs) for cancer treatment are of considerable interest in the field of theranostics. However, developing SDDSs with early diagnostic capability, enhanced drug delivery and efficient biodegradability still remains a scientific challenge. Herein, we report near-infrared light and tumor microenvironment (TME), dual responsive as well as size-switchable nanocapsules. These nanocapsules are made of a PLGA-polymer matrix coated with Fe/FeO core-shell nanocrystals and co-loaded with chemotherapy drug and photothermal agent. Smartly engineered nanocapsules can not only shrink and decompose into small-sized nanodrugs upon drug release but also can regulate the TME to overproduce reactive oxygen species for enhanced synergistic therapy in tumors. In vivo experiments demonstrate that these nanocapsules can target to tumor sites through fluorescence/magnetic resonance imaging and offer remarkable therapeutic results. Our synthetic strategy provides a platform for next generation smart nanocapsules with enhanced permeability and retention effect, multimodal anticancer theranostics, and biodegradability.


Assuntos
Doxorrubicina/administração & dosagem , Sistemas de Liberação de Medicamentos/métodos , Raios Infravermelhos/uso terapêutico , Nanocápsulas/química , Neoplasias/terapia , Nanomedicina Teranóstica/métodos , Animais , Antineoplásicos/administração & dosagem , Antineoplásicos/química , Antineoplásicos/farmacocinética , Linhagem Celular Tumoral , Doxorrubicina/química , Doxorrubicina/farmacocinética , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Células NIH 3T3 , Nanopartículas/química , Neoplasias/metabolismo , Neoplasias/patologia , Polímeros/química , Microambiente Tumoral/efeitos dos fármacos , Microambiente Tumoral/efeitos da radiação , Ensaios Antitumorais Modelo de Xenoenxerto/métodos
11.
J Orthop Surg Res ; 14(1): 313, 2019 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-31533751

RESUMO

BACKGROUND: This study aimed to assess the efficacy of water-filtered infrared A (wIRA) in sacroiliitis in male patients with ankylosing spondylitis (AS) and the effect of wIRA therapy on serum vascular endothelial growth factor (VEGF). METHODS: One hundred twenty male AS patients with active sacroiliitis were randomly divided into wIRA group and control group. wIRA treatment was performed twice daily for 5 consecutive days with 24-h interval before switching the treatment (crossover design). Bath ankylosing spondylitis disease activity index (BASDAI) scores, pain visual analogue scale (VAS), and morning stiffness VAS were recorded prior to and after each treatment period. Additionally, C-reactive protein (CRP), serum VEGF, and resistance index (RI) of sacroiliac joints detected by ultrasonography were recorded at baseline and after the first and second treatment period, respectively. The efficacy was examined by using repeated measures analysis of variance (ANOVA). RESULTS: BASDAI, pain VAS, and morning stiffness VAS scores decreased significantly (P < 0.001) after wIRA treatment and no-wIRA treatment (control group), and the difference between the two groups was significant (P < 0.001). CRP declined and RI increased during the wIRA treatment as compared with the no-wIRA treatment (P < 0.001). The increase in RI was associated with improvement of pain VAS scores (P = 0.018), while serum VEGF was unaffected by the treatment. CONCLUSIONS: wIRA treatment achieved symptom and pain relief for AS patients with active sacroiliitis. wIRA treatment also improved RI revealed by ultrasonography, and this effect was associated with improved pain VAS scores.


Assuntos
Raios Infravermelhos/uso terapêutico , Sacroileíte/radioterapia , Espondilite Anquilosante/radioterapia , Fator A de Crescimento do Endotélio Vascular/sangue , Adulto , Proteína C-Reativa/metabolismo , Estudos Cross-Over , Humanos , Masculino , Pessoa de Meia-Idade , Medição da Dor/métodos , Amplitude de Movimento Articular , Articulação Sacroilíaca/diagnóstico por imagem , Articulação Sacroilíaca/fisiopatologia , Sacroileíte/sangue , Sacroileíte/diagnóstico por imagem , Sacroileíte/fisiopatologia , Índice de Gravidade de Doença , Espondilite Anquilosante/sangue , Espondilite Anquilosante/diagnóstico por imagem , Espondilite Anquilosante/fisiopatologia , Resultado do Tratamento , Ultrassonografia , Adulto Jovem
12.
Photobiomodul Photomed Laser Surg ; 37(10): 615-622, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31536464

RESUMO

Background: Parkinson's disease is a well-known neurological disorder with distinct motor signs and non-motor symptoms. Objective: We report on six patients with Parkinson's disease that used in-house built photobiomodulation (PBM) helmets. Methods: We used "buckets" lined with light-emitting diodes (LEDs) of wavelengths across the red to near-infrared range (i.e., 670, 810, and 850 nm; n = 5) or an homemade intranasal LED device (660 nm; n = 1). Progress was assessed by the patients themselves, their spouse, or their attending medical practitioners. Results: We found that 55% of the initial signs and symptoms of the six patients showed overall improvement, whereas 43% stayed the same and only 2% got worse. We also found that PBM did not target a specific sign or symptom, with both motor and nonmotor ones being affected, depending on the patient. Conclusions: In summary, our early observations are the first to note the impact of PBM on patients' signs and symptoms over an extended period, up to 24 months, and lays the groundwork for further development to clinical trial.


Assuntos
Lasers Semicondutores/uso terapêutico , Terapia com Luz de Baixa Intensidade/instrumentação , Terapia com Luz de Baixa Intensidade/métodos , Doença de Parkinson/diagnóstico , Doença de Parkinson/radioterapia , Idoso , Encéfalo/efeitos da radiação , Desenho de Equipamento , Seguimentos , Dispositivos de Proteção da Cabeça , Humanos , Raios Infravermelhos/uso terapêutico , Masculino , Pessoa de Meia-Idade , Testes Neuropsicológicos/normas , Medição de Risco , Amostragem , Índice de Gravidade de Doença , Fatores de Tempo , Resultado do Tratamento
13.
Medicine (Baltimore) ; 98(34): e16882, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31441863

RESUMO

BACKGROUND: Cancer-related fatigue (CRF) is the most common and distressing symptom associated with cancer treatment that breast cancer survivors (BCS) experience. We previously found that laser moxibustion may be efficacious for CRF. The primary aim of this study is to determine the specific efficacy of 10.6 µm infrared laser moxibustion on CRF. The secondary aim is to evaluate the effect of infrared laser moxibustion on co-existing symptoms that BCS experience. METHODS: We will conduct a randomized, sham-controlled, three-arm trial of infrared laser moxibustion (ILM) against sham ILM (SILM) and waitlist control (WLC) among BCS with moderate to severe fatigue. The two intervention groups will receive either real or sham infrared laser moxibustion on four acupoints (i.e., ST36 [bilateral], CV4, and CV6) for 20 minutes each session for 6 weeks (twice per week). The primary endpoint is the change in fatigue score from Baseline to Week 6 as measured by the Chinese version of the Brief Fatigue Inventory (BFI-C). Our secondary aim is to compare the severity of co-morbidities (e.g., depression, insomnia, and pain) among the 3 groups. DISCUSSION: The results of our trial will establish evidence for the efficacy of infrared laser moxibustion for CRF, a very common and challenging symptom. TRIAL REGISTRATION NUMBER: NCT03553355.


Assuntos
Neoplasias da Mama/complicações , Sobreviventes de Câncer/psicologia , Fadiga/terapia , Raios Infravermelhos/uso terapêutico , Moxibustão/métodos , China , Feminino , Humanos , Qualidade de Vida , Ensaios Clínicos Controlados Aleatórios como Assunto
14.
Dalton Trans ; 48(35): 13120-13124, 2019 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-31348472

RESUMO

Near-infrared photothermal-activated nanomaterials are emerging as a promising tool in precise cancer theranostics. This Frontier article highlights the recent advances of photothermal-activated nanoagents in biomedical applications, namely photothermal-initiated drug/contrast agent release, gene silencing, programmed targeting and gas theranostics. In the end, we give a perspective on the further development of photothermal-sensitive nanomedicines.


Assuntos
Raios Infravermelhos/uso terapêutico , Neoplasias/diagnóstico , Neoplasias/terapia , Fototerapia/métodos , Nanomedicina Teranóstica/métodos , Inativação Gênica/efeitos da radiação , Humanos , Neoplasias/genética
15.
J Vis Exp ; (147)2019 05 23.
Artigo em Inglês | MEDLINE | ID: mdl-31180361

RESUMO

Neuroscience is the study of how cells in the brain mediate various functions. Measuring protein expression in neurons and glia is critical for the study of neuroscience as cellular function is determined by the composition and activity of cellular proteins. In this article, we describe how immunocytochemistry can be combined with near-infrared high-resolution scanning to provide a semi-quantitative measure of protein expression in distinct brain regions. This technique can be used for single or double protein expression in the same brain region. Measuring proteins in this fashion can be used to obtain a relative change in protein expression with an experimental manipulation, molecular signature of learning and memory, activity in molecular pathways, and neural activity in multiple brain regions. Using the correct proteins and statistical analysis, functional connectivity among brain regions can be determined as well. Given the ease of implementing immunocytochemistry in a laboratory, using immunocytochemistry with near-infrared high-resolution scanning can expand the ability of the neuroscientist to examine neurobiological processes at a systems level.


Assuntos
Encéfalo/diagnóstico por imagem , Fluorescência , Raios Infravermelhos/uso terapêutico , Proteínas/metabolismo , Cintilografia/métodos , Animais , Encéfalo/patologia , Humanos , Roedores
16.
Int J Radiat Biol ; 95(9): 1326-1336, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31170016

RESUMO

Introduction: Near-infrared (NIR) and red-to-near-infrared (R/NIR) radiation are increasingly applied for therapeutic use. R/NIR-employing therapies aim to stimulate healing, prevent tissue necrosis, increase mitochondrial function, and improve blood flow and tissue oxygenation. The wide range of applications of this radiation raises questions concerning the effects of R/NIR on the immune system. Methods: In this review, we discuss the potential effects of exposure to R/NIR light on immune cells in the context of physical parameters of light. Discussion: The effects that R/NIR may induce in immune cells typically involve the production of reactive oxygen species (ROS), nitrogen oxide (NO), or interleukins. Production of ROS after exposure to R/NIR can either be inhibited or to some extent increased, which suggests that detailed conditions of experiments, such as the spectrum of radiation, irradiance, exposure time, determine the outcome of the treatment. However, a wide range of immune cell studies have demonstrated that exposure to R/NIR most often has an anti-inflammatory effect. Finally, photobiomodulation molecular mechanism with particular attention to the role of interfacial water structure changes for cell physiology and regulation of the inflammatory process was described. Conclusions: Optimization of light parameters allows R/NIR to act as an anti-inflammatory agent in a wide range of medical applications.


Assuntos
Inflamação/radioterapia , Raios Infravermelhos/uso terapêutico , Animais , Sangue/efeitos da radiação , Granulócitos/efeitos da radiação , Humanos , Inflamação/imunologia , Inflamação/metabolismo , Inflamação/patologia
19.
Poult Sci ; 98(10): 4269-4281, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31073593

RESUMO

This research examined how infrared beak treatment (IRBT), sloughing of the treated beak tissue, and the variations in beak shape that can occur post-IRBT impact the welfare and mortality of Lohmann LSL-Lite (LW) and Lohmann Brown (LB) pullets and hens. Two experiments were conducted and birds for both experiments were treated on the day of hatch. IRBT equipment settings were adjusted to create 4 specific beak shapes: shovel (SHV), step (STP), standard (STAN), and an untreated sham control (C). Experiment 1 pullets (n = 80 per strain) were reared in bioassay cages from 1 to 29 D of age (4 replicates per treatment). Data collected included time and presence of beak sloughing, pecking force, behavioral expression, and mortality. Experiment 2 pullets (n = 320 per strain) were reared in floor pens from 1 D to 18 wk of age (2 replicates per treatment) and then conventional cages from 18 to 60 wk of age (6 replicates per treatment). Data collected for Experiment 2 included behavioral expression, feather cover, comb damage, and mortality. Data were analyzed using PROC MIXED (SAS® 9.4) with Tukey's test to separate means. Differences were significant when P ≤ 0.05. IRBT and sloughing had no effect on pecking force or mortality throughout rearing. The variations in post-IRBT beak shape had minor effects on behavior. During rearing, STAN pullets were more active than C pullets but STP and STAN pullets performed less exploratory pecking. During the laying period, SHV and STP hens preened more than C hens. The IRBT treatments, regardless of beak shape, reduced feather loss, comb damage, and cannibalism-related mortality during the laying period. Overall, the results indicate that LW and LB pullets and hens can cope with the change in beak shape that occurs with IRBT, and that welfare is not negatively impacted if some variation in beak shape occurs.


Assuntos
Agressão , Bem-Estar do Animal , Bico/efeitos da radiação , Galinhas/fisiologia , Ingestão de Alimentos , Raios Infravermelhos/uso terapêutico , Criação de Animais Domésticos/métodos , Animais , Bico/anatomia & histologia , Bico/fisiologia , Canibalismo , Galinhas/anatomia & histologia , Feminino , Distribuição Aleatória
20.
Int J Radiat Biol ; 95(9): 1337-1345, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31140889

RESUMO

Aim: To explore the influence of electromagnetic fields (EMFs) on the cell cycle progression of MDA-MB-231 and MCF-7 breast cancer cell lines and to evaluate the radiosensitizing effect of magnetotherapy during therapeutic co-exposure to EMFs and radiotherapy. Material and methods: Cells were exposed to EMFs (25, 50 and 100 Hz; 8 and 10 mT). In the co-treatment, cells were first exposed to EMFs (50 Hz/10 mT) for 30 min and then to ionizing radiation (IR) (2 Gy) 4 h later. Cell cycle progression and free radical production were evaluated by flow cytometry, while radiosensitivity was explored by colony formation assay. Results: Generalized G1-phase arrest was found in both cell lines several hours after EMF exposure. Interestingly, a marked G1-phase delay was observed at 4 h after exposure to 50 Hz/10 mT EMFs. No cell cycle perturbation was observed after repeated exposure to EMFs. IR-derived ROS production was enhanced in EMF-exposed MCF-7 cells at 24 h post-exposure. EMF-exposed cells were more radiosensitive in comparison to sham-exposed cells. Conclusions: These results highlight the potential benefits of concomitant treatment with magnetotherapy before radiotherapy sessions to enhance the effectiveness of breast cancer therapy. Further studies are warranted to identify the subset(s) of patients who would benefit from this multimodal treatment.


Assuntos
Neoplasias da Mama/patologia , Campos Eletromagnéticos , Ciclo Celular/efeitos da radiação , Relação Dose-Resposta à Radiação , Humanos , Raios Infravermelhos/uso terapêutico , Células MCF-7 , Terapia de Campo Magnético , Estresse Oxidativo/efeitos da radiação , Projetos Piloto
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