Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 14.898
Filtrar
1.
Braz Oral Res ; 33: e0045, 2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31531560

RESUMO

The aim of this study was to investigate the effects of low-intensity pulsed ultrasound (LIPUS) on the osteogenic differentiation of dental follicle cells (DFCs) in vitro and on the regenerative effects of DFC-OsteoBoneTM complexes in vivo. DFCs were isolated and characterized. In the in vitro study, DFCs were cultured in an osteogenic medium in the presence or absence of LIPUS. The expression levels of ALP, Runx2, OSX, and COL-I mRNA were analyzed using real-time polymerase chain reaction (RT-PCR) on day 7. Alizarin red staining was performed on day 21. The state of the growth of the DFCs that were seeded on the scaffold at 3, 5, 7, and 9 days was detected by using a scanning electron microscope. In our in vivo study, 9 healthy nude mice randomly underwent subcutaneous transplantation surgery in one of three groups: group A, empty scaffold; group B, DFCs + scaffold; and group C, DFCs + scaffold + LIPUS. After 8 weeks of implantation, a histological analysis was performed by HE and Mason staining. Our results indicate that LIPUS promotes the osteogenic differentiation of DFCs by increasing the expression of the ALP, Runx2, OSX, and COL-I genes and the formation of mineralized nodules. The cells can adhere and grow on the scaffolds and grow best at 9 days. The HE and Mason staining results showed that more cells, fibrous tissue and blood vessels could be observed in the DFCs + scaffold + LIPUS group than in the other groups. LIPUS could promote the osteogenic differentiation of DFCs in vitro and promote tissue regeneration in a DFCs-scaffold complex in vivo. Further studies should be conducted to explore the underlying mechanisms of LIPUS.


Assuntos
Regeneração Óssea/efeitos da radiação , Saco Dentário/citologia , Osteogênese/efeitos da radiação , Terapia por Ultrassom/métodos , Ondas Ultrassônicas , Animais , Cerâmica , Saco Dentário/efeitos da radiação , Citometria de Fluxo , Camundongos Nus , Microscopia Eletrônica de Varredura , Distribuição Aleatória , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase em Tempo Real , Reprodutibilidade dos Testes , Fatores de Tempo
2.
Braz Oral Res ; 33: e079, 2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31531565

RESUMO

Cell therapy associated with guided bone regeneration (GBR) can be used to treat bone defects under challenging conditions such as osteoporosis. This study aimed to evaluate the effect of mesenchymal stem cells (MSCs) in combination with a poly(vinylidene-trifluoroethylene)/barium titanate (PVDF-TrFE/BT) membrane on bone repair in osteoporotic rats. Osteoporosis was induced in female rats by bilateral removal of the ovaries (OVX) or sham surgery (SHAM), and the osteoporotic condition was characterized after 5 months by microtomographic and morphometric analyses. Calvarial defects were created in osteoporotic rats that immediately received the PVDF-TrFE/BT membrane. After 2 weeks, bone marrow-derived MSCs from healthy rats, characterized by the expression of surface markers using flow cytometry, or phosphate-buffered saline (PBS) (Control) were injected into the defects and bone formation was evaluated 4 weeks post-injection by microtomographic, morphometric, and histological analyses. A reduction in the amount of bone tissue in the femurs of OVX compared with SHAM rats confirmed the osteoporotic condition of the experimental model. More bone formation was observed when the defects were injected with MSCs compared to that with PBS. The modification that we are proposing in this study for the classical GBR approach where cells are locally injected after a membrane implantation may be a promising therapeutic strategy to increase bone formation under osteoporotic condition.


Assuntos
Compostos de Bário/farmacologia , Regeneração Tecidual Guiada/métodos , Células-Tronco Mesenquimais/fisiologia , Osteogênese/efeitos dos fármacos , Osteoporose/terapia , Polivinil/farmacologia , Titânio/farmacologia , Animais , Compostos de Bário/química , Densidade Óssea , Regeneração Óssea/efeitos dos fármacos , Regeneração Óssea/fisiologia , Feminino , Citometria de Fluxo , Imagem Tridimensional , Células-Tronco Mesenquimais/química , Osteogênese/fisiologia , Osteoporose/fisiopatologia , Ovariectomia , Polivinil/química , Distribuição Aleatória , Ratos Wistar , Reprodutibilidade dos Testes , Fatores de Tempo , Titânio/química , Resultado do Tratamento
3.
Acta Cir Bras ; 34(7): e201900704, 2019 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-31531539

RESUMO

PURPOSE: The effects of resveratrol administration on calvarial bone defects with alloplastic graft material was investigated for osteoinductive reaction and bone development in rats. METHODS: Healthy male rats were randomly divided into 3 groups consisting of 10 rats. Groups were as follows: control (defect) group, defect + graft group, and defect + graft + resveratrol group. A calvarial bone defect was created in all groups, alloplastic bone grafts were applied to the defect in the 2nd and 3rd group, resveratrol (5 mg/kg/day) was added to the drinking water of the animals following graft application for 28 days in the 3rd group. RESULTS: Increase in osteoclasts and necrotic changes were observed histopathologically in the control group. In the 2nd group, reduction of inflammation, congestion of blood vessels, increased osteblastic activity, osteoinductive effect, progression of osteocyte development and increased collagen fibers in connective tissue were observed. In the 3rd group, osteoblasts seemed to secrete bone matrix and accelerate osteoinductive effect with increased osteopregenitor activity and positive osteopontin and osteonectin expressions. CONCLUSION: Resveratrol treatment was thought to be an alternative and supportive drug for implant application by inducing new bone formation in the calvaral defect region as a result of short-term treatment.


Assuntos
Regeneração Óssea/efeitos dos fármacos , Substitutos Ósseos/administração & dosagem , Transplante Ósseo/métodos , Resveratrol/administração & dosagem , Crânio/cirurgia , Animais , Substitutos Ósseos/uso terapêutico , Modelos Animais de Doenças , Esquema de Medicação , Masculino , Osseointegração/efeitos dos fármacos , Osteoblastos/efeitos dos fármacos , Osteogênese/efeitos dos fármacos , Osteonectina/administração & dosagem , Osteopontina/administração & dosagem , Ratos , Crânio/efeitos dos fármacos
5.
Acta Cir Bras ; 34(6): e201900601, 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31432992

RESUMO

PURPOSE: To evaluate PBS®MCIMMO cement in the filling of bone defects. METHODS: Thirty-six adult male Wistar rats were divided into three groups of twelve individuals each (group 1, group 2 and group 3). In all groups, a bone failure in the femur was induced, 2.0 mm wide and 7.0 mm deep. In group 1, the PBS®MCIMMO cement was applied to the bone defect produced and a titanium implant (CONNECTION®) 1.5 mm thick and 6 mm long was installed. In group 2, only the PBS® CIMMO cement was installed. In group 3, only bone failure was performed. Kruskal Wallis tests were performed to compare the mean area among the three groups. RESULTS: In all comparisons, significance was observed for group 2 (p = 0.0014-0.0026). CONCLUSION: The PBS®CIMMO cement induced bone neoformation, and integration between the newly formed bone, cement, and implant was observed.


Assuntos
Materiais Biocompatíveis/administração & dosagem , Cimentos para Ossos , Regeneração Óssea/fisiologia , Substitutos Ósseos/administração & dosagem , Cerâmica , Fêmur/cirurgia , Teste de Materiais , Animais , Estudos Longitudinais , Masculino , Modelos Animais , Estudos Prospectivos , Ratos , Ratos Wistar
6.
Life Sci ; 234: 116743, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31408660

RESUMO

AIMS: The present study aimed to investigate the mechanism of bone repair mediated by recombination BMP-2 (rhBMP-2)/recombination CXC chemokine ligand-13 (rhCXCL13)-loaded hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite. MATERIALS AND METHODS: Firstly, the biological activity of rhBMP-2 and rhCXCL13 released from the complex was investigated. Secondly, the effect of rhBMP-2 sustained release solution on ALP activity and rhCXCL13 sustained release solution on cell migration of rat bone marrow mesenchyme stem cells was tested. Thirdly, osteoblasts differentiation test, X-ray scoring and three-point bending test were performed. Finally, the mRNAs expression of osteogenic marker genes and the protein expression of Runx2 was tested by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting (WB), respectively. KEY FINDINGS: RhBMP-2 could significantly promote the proliferation and differentiation, and RhCXCL13 could promote the migration of rat bone marrow MSCs. Detection of ALP activity and calcium salt deposition showed that rhBMP-2 and rhCXCL13 could significantly improve the biological activity and promote cell differentiation ability. X-ray scoring of radius and flexural strength test showed that rhBMP-2 and rhCXCL13 could promote bone healing and improve the bending resistance of bone tissue. The in vitro molecular experiments including RT-PCR and WB further demonstrated the roles of rhBMP-2 and rhCXCL13 in bone formation and bone repair. SIGNIFICANCE: Our results indicated that the hollow HA microspheres/CS composite could be effective as a delivery vehicle for rhBMP-2 and rhCXCL13 in bone regeneration and bone repair. In this process, rhBMP-2 may promote bone regeneration by regulating bone marrow MSCs cells recruited by rhCXCL13.


Assuntos
Proteína Morfogenética Óssea 2/administração & dosagem , Quimiocina CXCL13/administração & dosagem , Quitosana/análogos & derivados , Preparações de Ação Retardada/química , Durapatita/química , Osteogênese/efeitos dos fármacos , Tecidos Suporte/química , Fator de Crescimento Transformador beta/administração & dosagem , Animais , Materiais Biocompatíveis/química , Proteína Morfogenética Óssea 2/farmacologia , Regeneração Óssea/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Quimiocina CXCL13/farmacologia , Humanos , Masculino , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Osteoblastos/citologia , Osteoblastos/efeitos dos fármacos , Coelhos , Ratos , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/farmacologia , Fator de Crescimento Transformador beta/farmacologia
7.
J Phys Chem Lett ; 10(15): 4185-4191, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31295998

RESUMO

The treatment of massive bone defects is still a significant challenge for orthopedists. Here we have engineered synthetic porous AuPd alloy nanoparticles (pAuPds) as a hyperthermia agent for in situ bone regeneration through photothermal therapy (PTT). After being swallowed by cells, pAuPds produced a mild localized heat (MLH) (40-43 °C) under the irradiation of a near-infrared laser, which can greatly accelerate cell proliferation and bone regeneration. Almost 97% of the cranial defect area (8 mm in diameter) was covered by the newly formed bone after 6 weeks of PTT. RNA sequencing analysis was used to obtain insight into the molecular mechanism of the MLH on cell proliferation and bone formation. These results demonstrated that the Wnt signaling pathway was involved in the MLH. This Letter provides a unique strategy with mild heat stimulation and high efficiency for in situ bone regeneration.


Assuntos
Ligas/química , Regeneração Óssea , Ouro/química , Nanopartículas Metálicas/química , Paládio/química , Animais , Materiais Biocompatíveis/química , Linhagem Celular , Proliferação de Células , Sobrevivência Celular , Hipertermia Induzida , Raios Infravermelhos , Nanopartículas Metálicas/administração & dosagem , Nanopartículas Metálicas/toxicidade , Camundongos , Fototerapia , Porosidade , Ratos , Crânio/patologia
8.
Journal of Oral Investigations ; 8(2): 59-72, jul.-dez. 2019. tab, graf
Artigo em Inglês | BBO - Odontologia | ID: biblio-1007330

RESUMO

Purpose: This systematic review evaluated the influence of the membrane type (resorbable collagen or non-resorbable expanded polytetrafluorethylene; e-PTFE) on the guidedbone regeneration associated to implant placement. Methods: Any type of clinical study or literature review was searched at MEDLINE/PubMed and Cochrane databases. Two independent reviewers screened titles/abstracts of articles and the full-text of potentially eligible studies. When data was available, pairwise meta-analysis was performed using random statistical model. Results: Nine studies met the inclusion criteria, considering 685 implants in 360 patients. Vertical bone regeneration did not differ with the use of the two membranes in 8 of the 9 studies included. Meta-analysis did not show either greater vertical bone gain or vertical bone loss after regeneration with any of the membranes. The results have shown a tendency of higher bone gain in horizontal guided-bone regeneration with nonresorbable e-PTFE membranes (reported by 2 of 3 studies). All studies clearly showed that both membranes were effective in increase bone volume. There was no clear tendency of any of the two membranes in cause more complications. Conclusion: resorbable collagen and nonresorbable e-PTFE membranes are similarly effective in vertical guided-bone regeneration; however, horizontal guided-bone regeneration seems to benefit with the use of non-resorbable ones. Both membranes showed to be effective in guided-bone regeneration and similar in regards to complications(AU)


Objetivo: Esta revisão sistemática avaliou a influência do tipo de membrana (colágeno reabsorvível ou politetrafluoretileno expandido não reabsorvível; e-PTFE) na regeneração óssea guia associada à colocação do implante. Métodos: Qualquer tipo de estudo clínico ou revisão de literatura foi pesquisada nas bases de dados MEDLINE / PubMed e Cochrane. Dois revisores independentes examinaram títulos / resumos de artigos e o texto completo de estudos potencialmente elegíveis. Quando os dados estavam disponíveis, a meta-análise pareada foi realizada usando modelo estatístico aleatório. Resultados: Nove estudos preencheram os critérios de inclusão, considerando 685 implantes em 360 pacientes. A regeneração óssea vertical não diferiu com o uso das duas membranas em 8 dos 9 estudos incluídos. Meta-análise não mostrou maior ganho ósseo vertical ou perda óssea vertical após a regeneração com qualquer uma das membranas. Os resultados mostraram uma tendência de maior ganho ósseo na regeneração óssea guiada horizontal com membranas de e-PTFE não reabsorvíveis (relatadas por 2 de 3 estudos). Todos os estudos mostraram claramente que ambas as membranas foram eficazes no aumento do volume ósseo. Não houve uma tendência clara de qualquer uma das duas membranas em causar mais complicações. Conclusão: o colágeno reabsorvível e as membranas não-reabsorvíveis de PTFE-e são igualmente eficazes na regeneração óssea guiada vertical; no entanto, a regeneração óssea guiada horizontal parece se beneficiar com o uso das membranas não reabsorvíveis. Ambas as membranas mostraram-se eficazes na regeneração óssea guiada e similares em relação às complicações(AU)


Assuntos
Politetrafluoretileno , Regeneração Óssea , Implantes Dentários
9.
J Photochem Photobiol B ; 197: 111515, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31255939

RESUMO

An extraordinary arrangement of research is as yet going on in the area of orthopedic implants advancement to determine different issues being looked by the engineering today. In spite of a few detriments of the orthopedic metallic inserts, they keep on being utilized, essentially as a result of their unrivaled mechanical properties. We investigated the conceivable utilization of silicon carbide (SiC) as a nano-ceramic covering material of titanium (Ti)-based all out femoral substitution implants. The thought is to keep wear garbage arrangement from the delicate titanium exterior. Silicon carbide is a hard and firmly holding bio-ceramic surface substance, and in light of these physico-chemical properties, it isn't actually degradable, just like the case with apatite (HA). To improve cytocompatibility and osseous-integration, we deposited anodized titanium nanotubes (TiO2) inserts, by electrochemical deposition method (EDM), with silicon carbide (SiC) with apatite (SiC@HA). The deposition was affirmed by SEM, while phase composition properties were assessed by XRD. Calcium affidavit, osteocalcin creation, and articulation of bone genes were essentially higher in rodent osteoblast cell culture on SiC@HA-covered anodized titanium nanotubes than in cells cultured on uncoated anodized titanium nanotubes. Implantation into rodent femurs likewise demonstrated that the SiC@HA-covered substance had unrivaled osseous-integration movement in correlation with that of customary inserts, as evaluated by in vivo tomography and histology. Therefore, anodized titanium nanotubes covered with SiC@HA holds guarantee as an orthopedic implant substance.


Assuntos
Regeneração Óssea , Compostos Inorgânicos de Carbono/química , Materiais Revestidos Biocompatíveis/química , Durapatita/química , Nanopartículas/química , Compostos de Silício/química , Titânio/química , Animais , Regeneração Óssea/efeitos dos fármacos , Osso e Ossos/metabolismo , Osso e Ossos/patologia , Adesão Celular/efeitos dos fármacos , Materiais Revestidos Biocompatíveis/farmacologia , Materiais Revestidos Biocompatíveis/uso terapêutico , Subunidade alfa 1 de Fator de Ligação ao Core/genética , Subunidade alfa 1 de Fator de Ligação ao Core/metabolismo , Fraturas do Fêmur/terapia , Regulação da Expressão Gênica/efeitos dos fármacos , Masculino , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Células-Tronco Mesenquimais/metabolismo , Osteoblastos/citologia , Osteoblastos/efeitos dos fármacos , Osteocalcina/metabolismo , Próteses e Implantes , Ratos
10.
J Biomed Nanotechnol ; 15(3): 581-592, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31165702

RESUMO

Significant progress in artificial bone grafts has been achieved in recent years. However, none of them has osteogenic ability that is close to autogenous bones. Hence, improving osteogenic ability of artificial bone is the most prominent and challenging task in this field. In addition, angiogenesis could provide a stable environment and nutrients for survival of the cells and also plays a crucial role to the success of bone transplantation. In the present study, we combined citrate polymer and bioactive glass together as hybrids at the molecular level (PEC-GS/BG), with the expectation of acquiring osteogenic ability and angiogenic ability to repair bone defect that could comparable to autogenous bones. In vitro and in vivo experiment on the femoral condyle critical defects model of Sprague-Dawley rats were conducted for a complete evaluation. In vivo, the bone mineral density (BMD) in defects was no significant difference between autogenous bone groups (517 ± 21 mg/cm³) and PEC-GS/BG groups (509 ± 21 mg/cm³) (p > 0 05) at 12 weeks post-surgery. The BMD of the femoral condyle in normal males at the same age was measured to be 557 ± 16 mg/cm³, only slightly higher than the above date, indicating a nearly complete repair of the defects. It was also found that PEC-GS/BG promoted angiogenesis due to it stimulated organism to release vascular endothecial growth factor (VEGF). PEC-GS/BG also showed great osteogenic ability that was close to autogeneous bones, but much better angiogenic ability. What's more, from both protein and cell levels, PEC-GS/BG accelerated differentiation and mineralization of MC3T3E-1 cells. Consequently, osteogenetic performance of PEC-GS/BG was almost same to autogenous bones in repairing bone defects. Considering the high demand in bone grafts and all the difficulties in autogeneous bone supply, the PEC-GS/BG hybrids developed in this study may open a new horizon for bone repair.


Assuntos
Regeneração Óssea , Vidro , Animais , Masculino , Ratos , Ratos Sprague-Dawley
11.
J Biomed Nanotechnol ; 15(3): 602-611, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31165704

RESUMO

Interpenetrating network structures from Graphene foam (GF) and 58S bioactive glass (BG) are synthesized to combine the highly mechanical stability and conductivity from graphene with the superb bioactivity and biocompatibility from 58S BG. GF/58S BG scaffolds were prepared via multiple steps including chemical vapor deposition (CVD), spin-coating, and freeze drying methods. Simulated body fluid test confirms the highly bioactivity of the as-synthesized GF/58S BG scaffold after incorporating of sol-gel derived 58S BG. The GF/58S BG scaffold also remains good electrical conductivity of graphene after combination of 58S BG. Biocompatibility of both GF and GF/58S BG scaffold against the rabbit mesenchymal stem cells (rMSCs) is studied. Both GF and GF/58S BG scaffold facilitate the adhesion and extension of rMSCs, while the GF/58S BG scaffold shows a higher proliferation. Electrical stimulation was further applied on the both GF and GF/58S BG scaffold. Both scaffolds promote the osteogenic differentiation of rMSCs, while GF is more sensitive to the alternating electrical current. In vivo results based on the critical-sized radius defect rabbit model confirmed that the resulting GF/58S BG scaffold considerably promoted the formation of new bone. Our studies suggest that the assynthesized GF/58S BG scaffolds are the promising candidates for bone tissue engineering and electrically stimulated regeneration considering unique bioactive, biocompatible, conductive and stable properties of the resulting nanoscaffolds.


Assuntos
Estimulação Elétrica , Grafite , Células-Tronco Mesenquimais , Animais , Regeneração Óssea , Diferenciação Celular , Vidro , Osteogênese , Coelhos , Tecidos Suporte
12.
Altern Ther Health Med ; 25(4): 32-39, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31202209

RESUMO

Background and Context: Natural healing of bone lesions once incomplete or delayed bone regeneration represents an important clinical issue and plants possess compounds that may enhance bone healing, and avoid bone losses. Objective: The aim of this review is to evaluate the potential role of medicinal plants in the bone regenaration. Methods/Design: This review has included relevant studies available in MEDLINE-PubMed in the last three years that associated the role of plants in the bone regeneration. The descriptors used were "bone regeneration and plants" and "bone regeneration and medicinal plants". Results: We selected 59 articles, but only 15 studies dovetailed the study objectives of this review. These studies showed that plants have potential in increasing in the osseointegration once their components may downregulate biomarkers such as interleukin (IL)-1ß, IL-6, IL-8, Tumor Necrosis Factor-α, Metalloproteinase 2, and 3. They may also upregulate mediators such as Vascular Endothelial Growth Factor, Transforming-Growing Factor-ß1, Bone Morphogenetic Protein-2, osteocalcin, osteopontin, and type 1 collagen. The control in the production of these cytokines may help bone regeneration. Plant components such as curcumol, caffeic acid, resveratrol, luteolin, and many others may also be useful in bone health once may interfere in Nuclear Factor-κB and Mitogen Activated Protein Kinases, and may modulate Ca2+ signaling, inflammatory mediator genes, and inhibiting osteoclast-mediated bone resorption. Conclusion: Many plants possess components that are effective in promoting bone regeneration and new pharmaceutical technology and pharmacological researchers should be performed in order to establish the dose and the appropriate delivery vehicle of administration of the plant or its compounds.


Assuntos
Regeneração Óssea , Plantas Medicinais , Cicatrização/fisiologia , Humanos , Metaloproteinase 2 da Matriz , Fator A de Crescimento do Endotélio Vascular
13.
Int J Nanomedicine ; 14: 3929-3941, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31213809

RESUMO

Introduction: Hierarchical nanofibrous scaffolds are emerging as a promising bone repair material due to their high cell adhesion activity and nutrient permeability. However, the existing method for hierarchical nanofibrous scaffolds fabrication is complicated and not perfectly suitable for further biomedical application in view of both structure and function. In this study, we constructed a hierarchical nanofibrous poly (l-lactic acid)/poly(ε-caprolactone) (PLLA/PCL) scaffold and further evaluated its bone healing ability. Methods: The hierarchical PLLA/PCL nanofibrous scaffold (PLLA/PCL) was prepared by one-pot TIPS and then rapidly mineralized at room temperature by an electrochemical deposition technique. After electrode-positioning at 2 V for 2 hrs, a scaffold coated with hydroxyapatite (M-PLLA/PCL) could be obtained. Results: The pore size of the M-PLLA/PCL scaffold was hierarchically distributed so as to match the biophysical structure for osteoblast growth. The M-PLLA/PCL scaffold showed better cell proliferation and osteogenesis activity compared to the PLLA/PCL scaffold. Further in vivo bone repair studies indicated that the M-PLLA/PCL scaffold could accelerate defect healing in 12 weeks. Conclusion: The results of this study implied that the as-prepared hydroxyapatite coated hierarchical PLLA/PCL nanofibrous scaffolds could be developed as a promising material for efficient bone tissue repair after carefully tuning the TIPS and electrodeposition parameters.


Assuntos
Regeneração Óssea/fisiologia , Galvanoplastia/métodos , Minerais/química , Nanofibras/química , Copolímero de Ácido Poliláctico e Ácido Poliglicólico/farmacologia , Tecidos Suporte/química , Fosfatase Alcalina/metabolismo , Animais , Adesão Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Eletricidade , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Células-Tronco Mesenquimais/ultraestrutura , Osteogênese/efeitos dos fármacos , Porosidade , Ratos Sprague-Dawley , Crânio/diagnóstico por imagem , Crânio/efeitos dos fármacos , Crânio/patologia , Fatores de Tempo , Engenharia Tecidual/métodos , Microtomografia por Raio-X
14.
Carbohydr Polym ; 219: 210-218, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31151519

RESUMO

Scaffold plays a critical role in stem cell differentiation and tissue regeneration. Composite scaffolds composed of bacterial cellulose (BC) and collagen (Col) in different ratios (1:1, 3:1, 5:1) were fabricated in this study. The composite scaffolds exhibit a well-organized interconnected porous structure, significantly better physical stability than Col scaffold, and more water uptake up to 400%. They were also favorable with cell attachment and growth. After osteogenic induction of umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) for 3 weeks, we found more up-regulated osteogenic markers (collagen type 1, osteocalcin, bone sialoprotein) and significantly elevated proteins and calcium deposition, particularly with BC/Col (5:1) scaffold. When PKH-26 pre-labelled MSC-loaded scaffolds were subcutaneously transplanted in a mouse model, they showed many PKH-26-labelled cells and positive signals of α-smooth muscle actin, for neovascularization in the BC/Col (5:1). The current work demonstrates that our BC/Col composites may be promising as a bone tissue-engineered scaffold.


Assuntos
Celulose/química , Colágeno/química , Gluconacetobacter xylinus/metabolismo , Engenharia Tecidual/métodos , Tecidos Suporte/química , Animais , Regeneração Óssea/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Celulose/uso terapêutico , Colágeno/uso terapêutico , Humanos , Células-Tronco Mesenquimais/efeitos dos fármacos , Camundongos , Células NIH 3T3 , Osteogênese/efeitos dos fármacos
15.
J Craniofac Surg ; 30(4): 1308-1313, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31163569

RESUMO

The aim of this study was to investigate the effects of Bioglue as a mechanical barrier with or without biphasic calcium phosphate (BCP) in a rat tibia model. Sixty Sprague Dawley male rats weighing 250 ±â€Š20 g and 10 to 12 weeks of age were studied. Unicortical defects were created on the right tibia of all rats. Subjects were randomly divided into 3 groups. BioGlue group (24 rats); BioGlue alone, Graft group (24 rats); BioGlue + BCP and Control group; unfilled and uncovered (12 rats). Animals were euthanized at 7th, 21st, and 45th days postoperatively for histological and histomorphometric analyses. BioGlue material exhibited no adverse effects until the end of observation period. Bone-healing scores did not differ statistically between Control and BioGlue group, but found to be lower in Graft group on 21st and 45th days, (P < 0.001 and P < 0.01 on the 21st day and P < 0.01 and P < 0.05 on the 45th day, respectively). New bone formation in Graft group was found to be statistically different from Control group on the 7th and 21st days (P < 0.01 and P < 0.05 respectively), whereas no statistical difference was observed between BioGlue and Control group at all times. The present analysis indicates that BioGlue functioned well as a mechanical barrier allowing new bone formation. No additional benefit of combination treatment was detected in this study design and BCP did not offer any advantage for bone regeneration, thus it can serve as only a space maintainer.


Assuntos
Regeneração Óssea/efeitos dos fármacos , Hidroxiapatitas/farmacologia , Proteínas/farmacologia , Adesivos Teciduais/farmacologia , Animais , Substitutos Ósseos/farmacologia , Fosfatos de Cálcio/farmacologia , Colágeno , Masculino , Osteogênese/efeitos dos fármacos , Distribuição Aleatória , Ratos Sprague-Dawley , Tíbia/cirurgia
16.
Int J Nanomedicine ; 14: 3471-3490, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31190805

RESUMO

Background: Zinc-doped hydroxyapatite has been proposed as a graft biomaterial for bone regeneration. However, the effect of zinc on osteoconductivity is still controversial, since the release and resorption of calcium, phosphorus, and zinc in graft-implanted defects have rarely been studied. Methods: Microspheres containing alginate and either non-doped carbonated hydroxyapatite (cHA) or nanocrystalline 3.2 wt% zinc-doped cHA (Zn-cHA) were implanted in critical-sized calvarial defects in Wistar rats for 1, 3, and 6 months. Histological and histomorphometric analyses were performed to evaluate the volume density of newly formed bone, residual biomaterial, and connective tissue formation. Biomaterial degradation was characterized by transmission electron microscopy (TEM) and synchrotron radiation-based X-ray microfluorescence (SR-µXRF), which enabled the elemental mapping of calcium, phosphorus, and zinc on the microsphere-implanted defects at 6 months post-implantation. Results: The bone repair was limited to regions close to the preexistent bone, whereas connective tissue occupied the major part of the defect. Moreover, no significant difference in the amount of new bone formed was found between the two microsphere groups. TEM analysis revealed the degradation of the outer microsphere surface with detachment of the nanoparticle aggregates. According to SR-µXRF, both types of microspheres released high amounts of calcium, phosphorus, and zinc, distributed throughout the defective region. The cHA microsphere surface strongly adsorbed the zinc from organic constituents of the biological fluid, and phosphorus was resorbed more quickly than calcium. In the Zn-cHA group, zinc and calcium had similar release profiles, indicating a stoichiometric dissolution of these elements and non-preferential zinc resorption. Conclusions: The nanometric size of cHA and Zn-cHA was a decisive factor in accelerating the in vivo availability of calcium and zinc. The high calcium and zinc accumulation in the defect, which was not cleared by the biological medium, played a critical role in inhibiting osteoconduction and thus impairing bone repair.


Assuntos
Alginatos/química , Regeneração Óssea , Cálcio/metabolismo , Durapatita/química , Microesferas , Nanopartículas/química , Zinco/química , Zinco/metabolismo , Animais , Materiais Biocompatíveis/química , Disponibilidade Biológica , Regeneração Óssea/efeitos dos fármacos , Carbonatos/química , Morte Celular , Linhagem Celular , Sobrevivência Celular , Feminino , Camundongos , Nanopartículas/ultraestrutura , Ratos Wistar , Crânio/fisiologia , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
17.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 37(3): 325-329, 2019 Jun 01.
Artigo em Chinês | MEDLINE | ID: mdl-31218871

RESUMO

Guided bone regeneration (GBR) is an important technique to solve bone defect problems. In this technique, GBR barrier membranes play an irreplaceable role. GBR membranes can act as a barrier protecting fibroblasts from bone defects and promote osteoblast adhesion and proliferation, leading to bone regeneration. GBR barrier membranes should be enhanced because of the disadvantages of collagen membranes, which are extensively applied to the field of GBR. Therefore, various efforts have been devoted to modifying the antibacterial and osteogenic properties of GBR barrier membranes and developing novel materials. This article reviews the research advancements on the modification of GBR barrier membranes and discover future directions for the development of GBR barrier membranes to provide a reference for bone tissue engi-neering and repair.


Assuntos
Regeneração Óssea , Membranas Artificiais , Colágeno , Osteoblastos , Osteogênese
19.
J Appl Oral Sci ; 27: e20180621, 2019 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-31215599

RESUMO

OBJECTIVE: Considering the global public health problem of smoking, which can negatively influence bone tissue repair, the aim of this study is to analyze the influence of photobiomodulation therapy (PBM) on calvaria defects created surgically in specimens under the effect of cigarette smoke and analyzed with use of histomorphometric and immunohistochemistry techniques. METHODOLOGY: Calvaria defects 4.1 mm in diameter were surgically created in the calvaria of 90-day-old rats (n=60) that were randomly divided into 4 experimental groups containing 15 animals each: control group (C), smoking group (S), laser group (L), and smoke associated with laser group (S+L). The animals were subjected to surgery for calvaria defects and underwent PBM, being evaluated at 21, 45, and 60 days post-surgery. The specimens were then processed for histomorphometric and immunohistochemistry analyses. The area of bone neoformation (ABN), percentage of bone neoformation (PBNF), and the remaining distance between the edges of the defects (D) were analyzed histometrically. Quantitative analysis of the TRAP immunolabeled cells was also performed. The data were subjected to analysis of variance (ANOVA) in conjunction with Tukey's test to verify the statistical differences between groups (p<0.05). RESULTS: The smoking group showed less ABN compared to the other experimental groups in all periods, and it also showed more D at 21 days compared to the remaining groups and at 45 days compared to the laser group. The smoking group showed a lower PNBF compared to the laser group in all experimental periods and compared to smoking combined with LLLT group at 21 days. CONCLUSIONS: PBM acted on bone biomodulation, thus stimulating new bone formation and compensating for the negative factor of smoking, which can be used as a supportive therapy during bone repair processes.


Assuntos
Regeneração Óssea/efeitos dos fármacos , Fumar Cigarros/fisiopatologia , Terapia com Luz de Baixa Intensidade/métodos , Crânio/efeitos da radiação , Cicatrização/efeitos da radiação , Animais , Fumar Cigarros/efeitos adversos , Feminino , Imuno-Histoquímica , Osteogênese/efeitos dos fármacos , Distribuição Aleatória , Ratos Wistar , Reprodutibilidade dos Testes , Crânio/patologia , Fatores de Tempo , Resultado do Tratamento
20.
Niger J Clin Pract ; 22(6): 754-762, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31187758

RESUMO

Background: Reconstruction of bone defects in oral and maxillofacial surgery has widespread uses. In recent years, the capacity of various biomaterials alone or in combination with bone graft materials to increase bone healing has been an intensive research topic. The aim of this study is to evaluate the efficacy of hyaluronic acid and/or bone graft material on bone healing in defects created in the rat mandible. Methods: In our study, rats were divided into 4 groups. Group 1 is designated to be treated with no materials, Group 2 with graft material, Group 3 with only hyaluronic acid, and Group with hyaluronic acid and graft material. A critical-size defect of 5 mm in diameter was created bilaterally in the rat mandibles and the rats were divided into the indicated groups accordingly. At the end of the postoperative 6th week, the experiment was terminated. The right halves of the mandibles were evaluated immunohistochemically and histopathologically in terms of bone healing, and the left in terms of mineralization level via microcomputed tomography. Results: Histopathological evaluation showed that healing in the empty group was significantly lower than the other groups that were treated with materials (P < 0.05); but the difference between the material-treated groups was not significant. Immunohistochemical evaluation revealed that the staining was moderately positive/strongly positive in all groups, but the difference between the groups was not significant. The highest mineralization values observed in the defected areas that belonged to 2 groups using hyaluronic acid, and the difference between them was found to be statistically significant (P < 0.05). The lowest mineralization values observed in the defected areas was most frequent in the group where only the hyaluronic acid was used, and there was a statistically significant difference between the other groups (P < 0.05). Conclusion: In conclusion, the use of hyaluronic acid alone or in combination with bone grafting has been shown to contribute positively to the improvement of bone defects in the jaw area.


Assuntos
Materiais Biocompatíveis/uso terapêutico , Transplante Ósseo , Ácido Hialurônico/uso terapêutico , Doenças Mandibulares/cirurgia , Cicatrização , Animais , Regeneração Óssea/efeitos dos fármacos , Calcificação Fisiológica/efeitos dos fármacos , Masculino , Mandíbula/diagnóstico por imagem , Mandíbula/fisiopatologia , Doenças Mandibulares/diagnóstico por imagem , Doenças Mandibulares/patologia , Ratos , Cicatrização/efeitos dos fármacos , Microtomografia por Raio-X
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA