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1.
Bioresour Technol ; 293: 122072, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31484102

RESUMO

Based on the multi-functional magnetic sludge-derived biochar (MSBC), an innovative integrated process-coupling accumulation by adsorbing, degradation by microwave (MW)-induced catalytic oxidation in the presence of H2O2 and the regeneration of adsorbent simultaneously, was proposed. In this study, bisphenol S (4,4'-sulfonyldiphenol) was chosen as the pollutant model, its behaviors and related mechanism of BPS and MSBC in MW + H2O2 system were investigated. The BPS effective degradation on MSBC was proved by decoupling the adsorption and degradation with solvent extraction. OH and h+ play vital roles based on the scavenger tests. The synergistic effects of hot-spot of microwave irradiation, activation of H2O2, and charge transfer-induced doping effects of MSBC were attributed to the reactions. This work proves the feasibility in economics and energy-save treatment approach for low concentration organic pollutants in water.


Assuntos
Peróxido de Hidrogênio , Esgotos , Carvão Vegetal , Micro-Ondas , Fenóis , Regeneração , Sulfonas
2.
Adv Exp Med Biol ; 1167: 65-85, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31520349

RESUMO

Apoptosis has long been regarded as a tumor suppressor mechanism and evasion from apoptosis is considered to be one hallmark of cancer. However, this principle is not always consistent with clinical data which often illustrate a correlation between apoptosis and poor prognosis. Work in the last 15 years has provided an explanation for this apparent paradox. Apoptotic cells communicate with their environment and can produce signals which promote compensatory proliferation of surviving cells. This behavior of apoptotic cells is important for tissue regeneration in several model organisms, ranging from hydra to mammals. However, it may also play an important feature for tumorigenesis and tumor relapse. Several distinct forms of apoptosis-induced compensatory proliferation (AiP) have been identified, many of which involve reactive oxygen species (ROS) and immune cells. One type of AiP, "undead" AiP, in which apoptotic cells are kept in an immortalized state and continuously divide, may have particular relevance for tumorigenesis. Furthermore, given that chemo- and radiotherapy often aim to kill tumor cells, an improved understanding of the effects of apoptotic cells on the tumor and the tumor environment is of critical importance for the well-being of the patient. In this review, we summarize the current knowledge of AiP and focus our attention on recent findings obtained in Drosophila and other model organisms, and relate them to tumorigenesis.


Assuntos
Apoptose , Carcinogênese , Proliferação de Células , Neoplasias/patologia , Animais , Humanos , Espécies Reativas de Oxigênio , Regeneração
3.
Adv Exp Med Biol ; 1178: 155-174, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31493227

RESUMO

Skin aging is a complex process induced by intrinsic and extrinsic factors and causes alterations to the structural and functional aspects of the skin. Skin aging affects patients physically and physiologically. Understanding the process of skin aging can provide new knowledge on how to attenuate or reduce skin disorder symptoms. Herbs have been used for ages to prevent and treat skin aging, yet there are growing interests by researchers in this field globally. Various strategies have been developed for improving the quality and effectivity of herbal skin care products, both for topical and oral applications. This review will provide an overview of the relationship between herbal skin care products and the skin aging process.


Assuntos
Preparações de Plantas , Regeneração , Envelhecimento da Pele , Fenômenos Fisiológicos da Pele , Humanos , Preparações de Plantas/farmacologia , Regeneração/efeitos dos fármacos , Envelhecimento da Pele/efeitos dos fármacos , Higiene da Pele , Fenômenos Fisiológicos da Pele/efeitos dos fármacos
4.
Kardiologiia ; 59(8S): 15-23, 2019 Sep 16.
Artigo em Russo | MEDLINE | ID: mdl-31526358

RESUMO

Theory of atherogenesis and its complications underwent numerous changes. Today we observe that inflammation is a universal pathogenetic link between various processes such as atherosclerosis, rupture of atherosclerotic plaques and following myocardial infarction, post-infarction cardiac repair and heart failure. This review discusses examples, difficulties, and prospects of implementation of anti-inflammatory therapies in management of acute coronary syndrome and its complications.


Assuntos
Síndrome Coronariana Aguda , Humanos , Inflamação , Infarto do Miocárdio , Placa Aterosclerótica , Regeneração
5.
BMC Plant Biol ; 19(1): 373, 2019 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-31445524

RESUMO

BACKGROUND: The ability of severed rootstocks and shoots to re-establish vascular connections is used to generate grafted plants that combine desirable traits from both scions and rootstocks. Clarifying the mechanisms of graft healing is essential for its further application. We performed RNA sequencing of internodes near the cut position, making a distinction between separated or grafted tissues above and below the cut, in order to obtain a genetic description of graft union formation. RESULTS: Using weighted gene co-expression analysis, variable transcripts were clustered into 10 distinct co-expression networks (modules) based on expression profiles, and genes with the most "hubness" ("hub" genes show the most connections in a network) within each module were predicted. A large proportion of modules were related to Position, and represent asymmetric expression networks from different pathways. Expression of genes involved in auxin and sugar transport and signaling, and brassinosteroid biosynthesis was increased above the cut, while stress response genes were up-regulated below the cut. Some modules were related to graft union formation, among which oxidative detoxification genes were co-expressed along with both wounding response and cell wall organization genes. CONCLUSIONS: The present work provides a comprehensive understanding of graft healing-related gene networks in tomato. Also, the candidate pathways and hub genes identified here will be valuable for future studies of grafting in tomato.


Assuntos
Lycopersicon esculentum/fisiologia , Regeneração/genética , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Redes Reguladoras de Genes , Lycopersicon esculentum/genética , Raízes de Plantas/fisiologia , Transdução de Sinais
6.
Gene ; 717: 144047, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31421190

RESUMO

BACKGROUND: Vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF) signaling pathways play important roles in the formation of the blood vascular system and nervous system across animal phyla. We have earlier reported VEGF and FGF from Hydra vulgaris Ind-Pune, a cnidarian with a defined body axis, an organized nervous system and a remarkable ability of regeneration. We have now identified three more components of VEGF and FGF signaling pathways from hydra. These include FGF-1, FGF receptor 1 (FGFR-1) and VEGF receptor 2 (VEGFR-2) with a view to deciphering their possible roles in regeneration. METHODS: In silico analysis of proteins was performed using Clustal omega, Swiss model, MEGA 7.0, etc. Gene expression was studied by whole mount in situ hybridization. VEGF and FGF signaling was inhibited using specific pharmacological inhibitors and their effects on head regeneration were studied. RESULTS: Expression patterns of the genes indicate a possible interaction between FGF-1 and FGFR-1 and also VEGF and VEGFR-2. Upon treatment of decapitated hydra with pharmacological inhibitor of FGFR-1 or VEGFR-2 for 48 h, head regeneration was delayed in treated as compared to untreated, control regenerates. When we studied the expression of head specific genes HyBra1 and HyKs1 and tentacle specific gene HyAlx in control and treated regenerates using whole mount in situ hybridization, expression of all the three genes was found to be adversely affected in treated regenerates. CONCLUSIONS: The results suggest that VEGF and FGF signaling play important roles in regeneration of hypostome and tentacles in hydra.


Assuntos
Fatores de Crescimento de Fibroblastos/metabolismo , Cabeça/fisiologia , Hydra/fisiologia , Regeneração/fisiologia , Fator A de Crescimento do Endotélio Vascular/metabolismo , Animais , Simulação por Computador , Fator 1 de Crescimento de Fibroblastos/química , Fator 1 de Crescimento de Fibroblastos/genética , Fator 1 de Crescimento de Fibroblastos/metabolismo , Regulação da Expressão Gênica , Humanos , Hydra/efeitos dos fármacos , Indóis/farmacologia , Domínios Proteicos , Pirróis/farmacologia , Receptor Tipo 1 de Fator de Crescimento de Fibroblastos/química , Receptor Tipo 1 de Fator de Crescimento de Fibroblastos/genética , Receptor Tipo 1 de Fator de Crescimento de Fibroblastos/metabolismo , Regeneração/efeitos dos fármacos , Transdução de Sinais , Homologia Estrutural de Proteína , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/química , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/genética , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/metabolismo
7.
Sheng Li Xue Bao ; 71(4): 581-587, 2019 Aug 25.
Artigo em Chinês | MEDLINE | ID: mdl-31440755

RESUMO

The purpose of this study was to establish a method to record the dynamic process of vascular regeneration and remodeling in rat cerebral ischemic regions. An animal brain window model was established to continuously observe the changes of rat cortical vascular ischemia in vivo, and the model of cerebral ischemia was established by photochemical embolization. Optical coherence tomography (OCT) was performed to record the formation of vascular blockage and the injury and regeneration of small vessels during cerebral ischemia recovery. The results showed that 30 min of laser irradiation could completely block the cortical vessels in rats. Within 24-48 h after ischemia, the degree of brain injury was the greatest, and the number of blood vessels in the ischemic region reached the minimum. Then the blocked blood vessels began to be dredged, and the small blood vessels around the ischemic area began to regenerate. Small blood vessels in the superficial/deep layers of the cortex disappeared significantly after laser irradiation. During 10 d after ischemia, the blocked blood vessels were gradually dredged and recovered. On the 10th day after laser irradiation, a large number of neovascularization appeared in the superficial layer of cortex, but the deep vessels did not recover. These results indicate that the method established in this study can observe the changes of blood vessel in cerebral ischemic region continuously, which lays a foundation for further quantitative study on the dynamics of embolized blood vessels and peripheral capillaries during the recovery of cerebral ischemia.


Assuntos
Isquemia Encefálica , Encéfalo/irrigação sanguínea , Córtex Cerebral/irrigação sanguínea , Regeneração , Animais , Ratos
8.
Zhonghua Yan Ke Za Zhi ; 55(7): 549-553, 2019 Jul 11.
Artigo em Chinês | MEDLINE | ID: mdl-31288358

RESUMO

Regeneration of the lens is an ideal strategy for cataract patients to restore their adjustable vision and to acquire excellent visual quality. However, mammalian lens regeneration is slow and incomplete, and functional lens regeneration cannot be achieved. The ability of lower amphibians (such as newts) to regenerate the lens provides the impetus for research on the regeneration of the lens of mammal. At present, the main form of mammalian lens regeneration is the differentiation of lens epithelial cells by capsular bag as a scaffold. In recent years, the continuous development of stem cell technology, tissue engineering and biological materials have made great progress in lens regeneration. This article describes the processes in the normal development of the lens, and reviews the research results of lens regeneration at home and abroad, and discusses the possibility of the new techniques and methods related to regenerative medicine applied to lens regeneration. It is hoped to help in the realization of rapid and complete regeneration of functional lens.(Chin J Ophthalmol, 2019, 55: 549-553).


Assuntos
Catarata , Cristalino , Animais , Humanos , Mamíferos , Regeneração , Salamandridae
9.
Zhongguo Zhong Yao Za Zhi ; 44(10): 2032-2037, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31355557

RESUMO

In order to accelerate the breeding of the excellent seedlings of Polygonatum cyrtonema,tissue culture system of P. cyrtonema was established through the comprehensive regulation of key factors( leaf age,leaf location,basic media and plant growth regulators) and cytological basis of callus formation and differentiation was analyzed through paraffin section. The results showed that the 30-day-old leaf base explanton medium MS+6-BA 1. 50 mg·L~(-1)+2,4-D 0. 20 mg·L~(-1) had the highest induction rate( 80. 00%). The callus was initiated from cells on leaf base epidermis and near cortex,formed by the differentiation of middle vascular bundle cells. The optimal medium for adventitious bud differentiation was MS+ 6-BA 4. 00 mg·L~(-1)+ 2,4-D 0. 20 mg·L~(-1) with the differentiation rate of90. 33%,and the average number of buds was 5. 16. The adventitious buds had two origin types: exogenous and endogenous origin,formed by callus proximal cells and callus internal meristemoid. The adventitious bud proliferation medium was screened by orthogonal design,which determined the optimum combination was MS+ 6-BA 2. 00 mg·L~(-1)+NAA 0. 10 mg·L~(-1) and MS+ 6-BA 2. 00 mg·L~(-1)+NAA 0. 20 mg·L~(-1). The tubers with three leaves were cut and inoculated in the medium 1/2 MS+IBA 2. 00 mg·L~(-1),showing the highest rooting rate of 94. 00%. The rooting seedlings transplanted into the peat-vermiculite( 1 ∶ 1) matrix grew healthy and the survival rate was over 85. 00%. This research provided a novel solution for large-scale cultivation of P. cyrtonema seedling.


Assuntos
Meios de Cultura , Polygonatum/crescimento & desenvolvimento , Plântula/crescimento & desenvolvimento , Técnicas de Cultura de Tecidos , Reguladores de Crescimento de Planta , Folhas de Planta/citologia , Regeneração
10.
Bioresour Technol ; 290: 121781, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31319210

RESUMO

As one of the most mature carbon capture technologies, thermal regeneration of rich CO2 absorption solvent is a crucial challenge due to its high energy consumption (typically in the range of 3-6 MJ/kg CO2). In this work, a concept of bio-regeneration was proposed using microalgae to convert bicarbonate (which is one of the dominant components in rich solution) into value-added biomass. To evaluate the performance of bio-regeneration, different rich solution (including NH4HCO3, KHCO3 and NaHCO3) were investigated. Experimental results indicated that NH4HCO3 could be a promising bicarbonate carrier for the proposed absorption-microalgae hybrid process, which had a higher biomass productivity (55.36 mg·L-1·d-1) compared to KHCO3 and NaHCO3 and carbon sequestration capacity could be up to 158.3 mg·L-1·d-1. Meanwhile, pH adjustment was an effective approach to further intensify the performance of hybrid process. As a result, bio-regeneration of solvents could be a promising alternative to the conventional thermal regeneration.


Assuntos
Microalgas , Biomassa , Dióxido de Carbono , Regeneração , Solventes
12.
Science ; 365(6451): 314-316, 2019 07 26.
Artigo em Inglês | MEDLINE | ID: mdl-31346049
13.
Fa Yi Xue Za Zhi ; 35(3): 280-284, 2019 Jun.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-31282620

RESUMO

Abstract: Objective To investigate the morphological changes in the degeneration and regeneration of neuromuscular junctions (NMJ) during the repair of mouse skeletal muscle contusion and discuss the correlation between the degeneration and regeneration of NMJ and wound age. Methods A total of 50 healthy adult male mice were randomly divided into 10 groups, including 9 experimental groups and 1 control group. Immunofluorescent staining was applied, and neurofilament was marked with neurofilament protein-H (NF-H), presynaptic membrane was marked with synaptophysin (Syn), presynaptic membrane was marked with acetylcholine receptor (AChR). Morphological changes of NMJ regeneration at different time points after mouse skeletal muscle contusion were detected. Results The neurofilament and presynaptic membrane of NMJ at the junction of contusion zones began to degrade after contusion, and completed degradation at about 3 d post-injury. Then they gradually regenerated, roughly completing the regeneration at about 21 d and basically reaching the control group level. The ratio of presynaptic membrane quantity to presynaptic membrane quantity showed a trend of decreasing then rising and finally reaching the control level. Conclusion During the repair of mouse skeletal muscle contusion, the morphological changes and wound age of the NMJ at the junction of contusion zones have a close correlation, which is expected to be one of the biological indicators for forensic skeletal muscle wound age estimation.


Assuntos
Contusões , Junção Neuromuscular , Animais , Masculino , Camundongos , Músculo Esquelético , Regeneração
14.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 41(3): 291-299, 2019 Jun 30.
Artigo em Chinês | MEDLINE | ID: mdl-31282321

RESUMO

Objective To induce adipose-derived stem cells (ADSCs) to differentiate into intermediate mesoderm (IM)-like cells in vitro,with IM-like cells for recellularizing kidney scaffolds,and then to obtain a tissue-engineering kidney with renal structures and functions through co-culture.Methods After inguinal fat pads of Wistar rats were surgically harvested,the primary ADSCs were isolated,induced,and cultured for stem cell identification. ADSCs were inducted to differentiate into IM-like cells by adding glycogen synthase kinase-3 inhibitor (CHIR99021) and fibroblast growth factor 9 (FGF9) at different stages. Seven days later,the IM-like cells were identified. The induced IM-like cells and well-prepared kidney decellularized scaffolds were co-cultured for 10 days to obtain recellularized tissue-engineered kidneys and their differentiation was identified.Results The ADSCs harvested had osteogenic and adipogenic abilities and could express the stem cell surface markers. After 7 days of in vitro induction,the positive expressions of odd-skipped related 1 and paired-box 2 were observed in IM-like cells by immunofluorescence technique. After 10 days of co-culture with kidney decellularized scaffolds,the positive expressions of Wilms'tumor 1,GATA-binding protein-3,and E-cadherin were observed by immunofluorescence technique.Conclusion ADSCs can be induced into IM-like cells,and renal cell differentiation can be observed through combining the induced IM-like cells with kidney decellularized scaffolds.


Assuntos
Rim/crescimento & desenvolvimento , Mesoderma/citologia , Regeneração , Células-Tronco/citologia , Engenharia Tecidual , Tecido Adiposo , Animais , Diferenciação Celular , Células Cultivadas , Ratos , Ratos Wistar , Tecidos Suporte
15.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 41(3): 425-429, 2019 Jun 30.
Artigo em Chinês | MEDLINE | ID: mdl-31282341

RESUMO

Human optic nerve injury and its associated neurodegenerative diseases are often followed by permanent vision loss.Stem cell therapy has long been considered a promising mode to treat retinal degenerative diseases.Recent studies revealed that there are silent retinal stem cells in the eyes of mammals and even humans.These stem cells can be activated again under certain conditions and differentiate into retinal neurons to repair the damaged retina.Wnt signaling pathway plays a crucial role in conducting growth-stimulating signals and regulates cell proliferation,differentiation and apoptosis.This article review the regulatory effect of Wnt signaling pathway on stem cells-based retinal regeneration and the sources of retinal stem cells.


Assuntos
Regeneração , Retina/crescimento & desenvolvimento , Células-Tronco/metabolismo , Via de Sinalização Wnt , Animais , Diferenciação Celular , Humanos
16.
J Nanobiotechnology ; 17(1): 82, 2019 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-31291960

RESUMO

Skin damages are defined as one of most common lesions people suffer from, some of wounds are notoriously difficult to eradicate such as chronic wounds and deep burns. Existing wound therapies have been proved to be inadequate and far from satisfactory. The cutting-edge nanotechnology offers an unprecedented opportunity to revolutionize and invent new therapies or boost the effectiveness of current medical treatments. In particular, the nano-drug delivery systems anchor bioactive molecules to applied area, sustain the drug release and explicitly enhance the therapeutic efficacies of drugs, thus making a fine figure in field relevant to skin regeneration. This review summarized and discussed the current nano-drug delivery systems holding pivotal potential for wound healing and skin regeneration, with a special emphasis on liposomes, polymeric nanoparticles, inorganic nanoparticles, lipid nanoparticles, nanofibrous structures and nanohydrogel.


Assuntos
Materiais Biocompatíveis/química , Sistemas de Liberação de Medicamentos/métodos , Nanopartículas/química , Fenômenos Fisiológicos da Pele , Cicatrização/efeitos dos fármacos , Animais , Liberação Controlada de Fármacos , Humanos , Hidrogéis/química , Lipídeos/química , Lipossomos/química , Polímeros/química , Regeneração
18.
Nature ; 571(7765): 398-402, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31292548

RESUMO

A decline in stem cell function impairs tissue regeneration during ageing, but the role of the stem-cell-supporting niche in ageing is not well understood. The small intestine is maintained by actively cycling intestinal stem cells that are regulated by the Paneth cell niche1,2. Here we show that the regenerative potential of human and mouse intestinal epithelium diminishes with age owing to defects in both stem cells and their niche. The functional decline was caused by a decrease in stemness-maintaining Wnt signalling due to production of Notum, an extracellular Wnt inhibitor, in aged Paneth cells. Mechanistically, high activity of mammalian target of rapamycin complex 1 (mTORC1) in aged Paneth cells inhibits activity of peroxisome proliferator activated receptor α (PPAR-α)3, and lowered PPAR-α activity increased Notum expression. Genetic targeting of Notum or Wnt supplementation restored function of aged intestinal organoids. Moreover, pharmacological inhibition of Notum in mice enhanced the regenerative capacity of aged stem cells and promoted recovery from chemotherapy-induced damage. Our results reveal a role of the stem cell niche in ageing and demonstrate that targeting of Notum can promote regeneration of aged tissues.


Assuntos
Envelhecimento , Senescência Celular , Esterases/metabolismo , Mucosa Intestinal/patologia , Celulas de Paneth/metabolismo , Regeneração , Envelhecimento/fisiologia , Animais , Senescência Celular/fisiologia , Esterases/antagonistas & inibidores , Esterases/biossíntese , Feminino , Humanos , Mucosa Intestinal/fisiologia , Masculino , Alvo Mecanístico do Complexo 1 de Rapamicina/metabolismo , Camundongos , PPAR alfa/metabolismo , Celulas de Paneth/patologia , Receptores Acoplados a Proteínas-G/metabolismo , Nicho de Células-Tronco , Células-Tronco/patologia , Proteínas Wnt/antagonistas & inibidores , Via de Sinalização Wnt
19.
J Biol Regul Homeost Agents ; 33(2 Suppl. 1): 89-95, 2019 Mar-Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31169015

RESUMO

Nowadays several studies demonstrate the influence of chemical and physical stimulation to bone and cartilage exist. The first studies date back to the 50s and for a long time, they did not have a strong impact on clinical practice. In recent times, however, the findings arising from these studies are increasingly used to address clinical problems such as osteoarthritis or non-unions. The aim of this article is to make a review of the literature of the state of the art about physical and chemical influences on bone and cartilage.


Assuntos
Desenvolvimento Ósseo , Cartilagem Articular/crescimento & desenvolvimento , Osteoartrite , Regeneração , Humanos
20.
Artigo em Chinês | MEDLINE | ID: mdl-31163539

RESUMO

Summary Deformity of the auricle can result from congenital defects, oncologic resection, or traumatic injury. Current clinical strategy is to utilize autologous costal cartilage for reconstructing auricular scaffolds with long-term stability. However, this method is limited by donor-site morbidity, a complex surgical sculpting process, and differing mechanical properties compared to elastic auricular cartilage. Alternatively, alloplastic implants can offer precise shape and avoid donor-site surgery, but suffer from poor biocompatibility and high rates of infection and extrusion. A tissue engineering approach to auricular reconstructions would overcome the limitations of both autologous and alloplastic transplants. In this review we focus on tissue engineering auricular cartilage reconstruction, including auricular seed cells culture and their differentiated control strategy, scaffolds choices and design technique. Many studies found that auricular chondrocytes were the most suitable seed cells to fabricate elastic cartilage; progenitor cells from auricular cartilage could differentiate into elastic chondrocytes, and other types of chondrocytes had its limitation because of the final ossification. Many types of growth factors had the differing potential ability for accelerating chondrocytes growth, differentiation, or maintaining chondrocytes phenotype. Recent advances in tissue engineering auricular cartilage, including several clinic applications, implied that tissue engineering auricules would become a hopefully potential soulution for the future of auricular reconstruction.


Assuntos
Cartilagem da Orelha , Medicina Regenerativa , Engenharia Tecidual , Condrócitos , Regeneração
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