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1.
Facial Plast Surg Clin North Am ; 30(2): 125-133, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35501049

RESUMO

Understanding the relevance of anatomic and biomechanical principles is crucial when treating the face with soft tissue fillers to achieve a symmetric, soft, and natural-looking result while mitigating the risk of adverse events. The objective of this study is to summarize facial age-related effects, to relate them to facial biomechanics, and to establish guidelines for safe, effective, and esthetically pleasing full-face treatment following 3 basic principles while incorporating the latest scientific developments. This narrative review summarizes the current understanding of facial aging and its implications for facial biomechanics deduced from the authors' experience and research.


Assuntos
Rejuvenescimento , Envelhecimento da Pele , Envelhecimento , Fenômenos Biomecânicos , Face , Humanos
2.
Sci Data ; 9(1): 197, 2022 05 10.
Artigo em Inglês | MEDLINE | ID: mdl-35538082

RESUMO

The gut microbiota is associated with the health and longevity of the host. A few methods, such as fecal microbiota transplantation and oral administration of probiotics, have been applied to alter the gut microbiome and promote healthy aging. The changes in host microbiomes still remain poorly understood. Here, we characterized both the changes in gut microbial communities and their functional potential derived from colon samples in mouse models during aging. We achieved this through four procedures including co-housing, serum injection, parabiosis, and oral administration of Akkermansia muciniphila as probiotics using bacterial 16 S rRNA sequencing and shotgun metagenomic sequencing. The dataset comprised 16 S rRNA sequencing (36,249,200 paired-end reads, 107 sequencing data) and metagenomic sequencing data (307,194,369 paired-end reads, 109 sequencing data), characterizing the taxonomy of bacterial communities and their functional potential during aging and rejuvenation. The generated data expand the resources of the gut microbiome related to aging and rejuvenation and provide a useful dataset for research on developing therapeutic strategies to achieve healthy active aging.


Assuntos
Envelhecimento , Microbioma Gastrointestinal , RNA Ribossômico 16S , Envelhecimento/genética , Animais , Modelos Animais de Doenças , Microbioma Gastrointestinal/genética , Metagenômica , Camundongos , RNA Ribossômico 16S/genética , Rejuvenescimento
3.
Skinmed ; 20(2): 97-104, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35532761

RESUMO

Autologous serum is a component taken from patient's blood after centrifugation to be used for the same patient. Lately, growth factors (GF) found in platelet-rich plasma (PRP) has been widely used as an alternative therapeutic modality in various medical fields. The benefits of using autologous serum effectively include reduced risk of hypersensitivity or allergic reactions as well as the reduced risk of transmission of infectious diseases; however, in practice, the availability of products with GF is still limited. This study aimed to review the latest evidences of using autologous serum therapy in dermatology. We searched and screened the study papers of past 5 years (2015 - 2020) through Pubmed Medline for the following topic: "Risks and benefits of autologous serum in the field of dermatology." The initial search obtained 333 papers, of which only 14 met the inclusion criteria: these included five papers on dermatology, seven on ophthalmology, and one paper each on plastic surgery and orthopedics. PRP serum contains GF, vitamins, hormones, and other components. GF contained in PRP is an effective therapeutic modality to be used in dermatology for wound healing, skin rejuvenation, acne scar, and androgenic alopecia. (SKINmed. 2022;20:97-104).


Assuntos
Dermatologia , Plasma Rico em Plaquetas , Alopecia/terapia , Cicatriz/terapia , Humanos , Rejuvenescimento
4.
Clin Plast Surg ; 49(2): 197-212, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35367030

RESUMO

The upper lid-brow junction is a complex anatomic zone that undergoes many interconnected age-related changes. Although considerable effort has gone into defining the ideal female eye and brow, no such work has been done for the male. Typically, men develop forehead and glabellar lines in conjunction with either upper lid hooding, brow ptosis, or blepharoptosis, whereas some men develop hollowing of the upper lid sulcus. Physical examination defines which features predominate. Treatment can be nonsurgical or surgical. The surgical options include upper lid blepharoplasty, various types of brow lifting or brow shaping, ptosis repair, and fat grafting.


Assuntos
Blefaroplastia , Ritidoplastia , Sobrancelhas , Pálpebras/cirurgia , Feminino , Humanos , Masculino , Rejuvenescimento
5.
Clin Plast Surg ; 49(2): 221-256, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35367032

RESUMO

Increasing numbers of men are seeking surgical rejuvenation of their face and plastic surgeons have come to recognize that male facial aesthetics differ from those in women and that attractive masculinity is not as closely correlated with youth and beauty as is femininity. As a result, men generally seek a somewhat different outcome from facelift surgery. This has led to a rethinking of techniques that have evolved mainly to treat facial aging in women, and techniques have emerged that allow rejuvenation of the male face while preserving a natural, masculine appearance free of signs that surgery has been performed.


Assuntos
Ritidoplastia , Adolescente , Beleza , Face/cirurgia , Feminino , Cabeça , Humanos , Masculino , Rejuvenescimento , Ritidoplastia/métodos
6.
Clin Plast Surg ; 49(2): 257-273, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35367033

RESUMO

Specialists seeking successful outcomes in male facial rejuvenation must be able to achieve adequate results in the neck and submental region to provide their patients with balanced and natural results. A thorough understanding of male jawline and neck surface aesthetics is described and its relevance to perceived age, attractiveness, and body mass index is presented. The neck lift technique described is based on the pursuit of 2 distinct objectives managed independently: (1) Volume contouring or reduction, which is mainly accomplished in the deep structures of the neck beneath the platysma and (2) superficial redraping, which consists of the management of the platysma itself and of the overlying subcutaneous fat and skin under minimal tension. A dual-plane approach to the neck is used, meaning 2 different dissection planes are carried out. In the area cranial to the submandibular-cervical junction line (ie, submandibular segment), a plane is developed both superficial and deep to the platysma, while in the area caudal to this line (ie, cervical segment), dissection is carried out only deep to the platysma, leaving the muscle attached to its overlying skin. A description of the technique is presented as well as its complications and indications for surgical neck enhancement.


Assuntos
Procedimentos Cirúrgicos Reconstrutivos , Ritidoplastia , Sistema Musculoaponeurótico Superficial , Humanos , Masculino , Pescoço/cirurgia , Procedimentos Cirúrgicos Reconstrutivos/métodos , Rejuvenescimento , Ritidoplastia/métodos , Sistema Musculoaponeurótico Superficial/cirurgia
7.
Aging (Albany NY) ; 14(7): 3325-3328, 2022 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-35417855

RESUMO

Parabiosis is a well-established method to facilitate a shared blood supply between two conjoined animals. In particular, the pairing of mice of dissimilar ages, termed heterochronic parabiosis, has been used extensively for differentiating cell autonomous and non-autonomous mechanisms of aging. Analysis of heterochronic parabionts also has helped to identify individual circulating factors that may act as either pro- or anti-geronics. Heterochronic parabiosis also has proven to be a valuable experimental system to evaluate the effects of specific hallmarks of aging on the process of aging. For example, heterochronic parabiosis was used recently to examine whether cellular senescence was driven via cell autonomous and/or non-autonomous mechanisms. As anticipated, markers of cellular senescence were elevated in old isochronically-paired mice relative to young controls. However, compared to old isochronically paired mice, the senescent cell burden was reduced in multiple tissues of old parabionts joined with young mice. This suggests that the rejuvenation of cells and tissues in old mice by exposure to young blood could be mediated, in part, through suppression or immune clearance of senescent cells. Conversely, young heterochronic parabionts showed increased markers of cellular senescence, demonstrating that exposure to an old circulation is able to drive senescence through a cell non-autonomous mechanism(s), likely contributing to accelerated aging in the young mice. Thus, heterochronic parabiosis is still an important methodology that should continue to be leveraged for evaluating other hallmarks of aging and their mechanisms.


Assuntos
Senescência Celular , Parabiose , Envelhecimento , Animais , Biomarcadores , Camundongos , Rejuvenescimento
8.
Elife ; 112022 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-35390271

RESUMO

Ageing is the gradual decline in organismal fitness that occurs over time leading to tissue dysfunction and disease. At the cellular level, ageing is associated with reduced function, altered gene expression and a perturbed epigenome. Recent work has demonstrated that the epigenome is already rejuvenated by the maturation phase of somatic cell reprogramming, which suggests full reprogramming is not required to reverse ageing of somatic cells. Here we have developed the first "maturation phase transient reprogramming" (MPTR) method, where reprogramming factors are selectively expressed until this rejuvenation point then withdrawn. Applying MPTR to dermal fibroblasts from middle-aged donors, we found that cells temporarily lose and then reacquire their fibroblast identity, possibly as a result of epigenetic memory at enhancers and/or persistent expression of some fibroblast genes. Excitingly, our method substantially rejuvenated multiple cellular attributes including the transcriptome, which was rejuvenated by around 30 years as measured by a novel transcriptome clock. The epigenome was rejuvenated to a similar extent, including H3K9me3 levels and the DNA methylation ageing clock. The magnitude of rejuvenation instigated by MPTR appears substantially greater than that achieved in previous transient reprogramming protocols. In addition, MPTR fibroblasts produced youthful levels of collagen proteins, and showed partial functional rejuvenation of their migration speed. Finally, our work suggests that optimal time windows exist for rejuvenating the transcriptome and the epigenome. Overall, we demonstrate that it is possible to separate rejuvenation from complete pluripotency reprogramming, which should facilitate the discovery of novel anti-ageing genes and therapies.


Assuntos
Células-Tronco Pluripotentes Induzidas , Rejuvenescimento , Reprogramação Celular/genética , Metilação de DNA , Epigenoma , Epigenômica/métodos , Fibroblastos , Humanos , Pessoa de Meia-Idade
9.
Int J Mol Sci ; 23(8)2022 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-35457154

RESUMO

Advances in aging studies brought about by heterochronic parabiosis suggest that agingmight be a reversable process that is affected by changes in the systemic milieu of organs andcells. Given the broadness of such a systemic approach, research to date has mainly questioned theinvolvement of "shared organs" versus "circulating factors". However, in the absence of a clearunderstanding of the chronological development of aging and a unified platform to evaluate thesuccesses claimed by specific rejuvenation methods, current literature on this topic remains scattered.Herein, aging is assessed from an engineering standpoint to isolate possible aging potentiators via ajuxtaposition between biological and mechanical systems. Such a simplification provides a generalframework for future research in the field and examines the involvement of various factors in aging.Based on this simplified overview, the kidney as a filtration organ is clearly implicated, for the firsttime, with the aging phenomenon, necessitating a re-evaluation of current rejuvenation studies tountangle the extent of its involvement and its possible role as a potentiator in aging. Based on thesefindings, the review concludes with potential translatable and long-term therapeutics for aging whileoffering a critical view of rejuvenation methods proposed to date.


Assuntos
Parabiose , Rejuvenescimento , Imunoterapia
10.
Nursing ; 52(5): 6, 2022 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-35452029
11.
J Drugs Dermatol ; 21(4): 387-392, 2022 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-35389598

RESUMO

Restylane® Lidocaine is one of the most widely used hyaluronic acid (HA) fillers to replace lost or displaced volume during tear trough correction. Patient goals for tear trough correction include looking less tired or removing dark circles and this may be achieved by administering HA filler into the infraorbital region to correct the lower eyelid relative to the volume deficit, thereby smoothing the transition from the lower eyelid to the cheek. To achieve patient satisfaction and consistent results with Restylane, optimal application is essential; however, clinical guidance based on experience is limited. This paper reflects the recommendations of an interdisciplinary expert panel for the use of Restylane in correcting tear trough deformity, including patient selection, dosing, injection technique, and post-treatment care. Recommendations were discussed and agreed as a consensus, according to cross-sectional expertise and clinical experience. J Drugs Dermatol. 2022;21(4):387-392. doi:10.36849/JDD.6597.


Assuntos
Técnicas Cosméticas , Preenchedores Dérmicos , Envelhecimento da Pele , Estudos Transversais , Preenchedores Dérmicos/efeitos adversos , Pálpebras , Humanos , Ácido Hialurônico/efeitos adversos , Rejuvenescimento
12.
Nature ; 603(7900): 309-314, 2022 03.
Artigo em Inglês | MEDLINE | ID: mdl-35236985

RESUMO

The ability to slow or reverse biological ageing would have major implications for mitigating disease risk and maintaining vitality1. Although an increasing number of interventions show promise for rejuvenation2, their effectiveness on disparate cell types across the body and the molecular pathways susceptible to rejuvenation remain largely unexplored. Here we performed single-cell RNA sequencing on 20 organs to reveal cell-type-specific responses to young and aged blood in heterochronic parabiosis. Adipose mesenchymal stromal cells, haematopoietic stem cells and hepatocytes are among those cell types that are especially responsive. On the pathway level, young blood invokes new gene sets in addition to reversing established ageing patterns, with the global rescue of genes encoding electron transport chain subunits pinpointing a prominent role of mitochondrial function in parabiosis-mediated rejuvenation. We observed an almost universal loss of gene expression with age that is largely mimicked by parabiosis: aged blood reduces global gene expression, and young blood restores it in select cell types. Together, these data lay the groundwork for a systemic understanding of the interplay between blood-borne factors and cellular integrity.


Assuntos
Parabiose , Rejuvenescimento , Células-Tronco Hematopoéticas , Mitocôndrias/genética
14.
Stem Cells Transl Med ; 11(3): 231-238, 2022 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-35303745

RESUMO

Stem cell therapies, including stem cell transplantation and rejuvenation of stem cells in situ, are promising avenues for tackling a broad range of diseases. Stem cells can both self-renew and differentiate into other cell types, and play a significant role in the regulation of tissue homeostasis and regeneration after cell degeneration or injury. However, stem cell exhaustion or dysfunction increases with age and impedes the normal function of multiple tissues and systems. Thus, stem cell therapies could provide a solution to aging and age-associated diseases. Here, we discuss recent advances in understanding the mechanisms that regulate stem cell regeneration. We also summarize potential strategies for rejuvenating stem cells that leverage intrinsic and extrinsic factors. These approaches may pave the way toward therapeutic interventions aiming at extending both health and life span.


Assuntos
Senescência Celular , Rejuvenescimento , Senescência Celular/fisiologia , Rejuvenescimento/fisiologia , Transplante de Células-Tronco
15.
Biochem Biophys Res Commun ; 603: 41-48, 2022 05 07.
Artigo em Inglês | MEDLINE | ID: mdl-35278878

RESUMO

An increasing number of studies have indicated that alterations in gut microbiota affect brain function, including cognition and memory ability, via the gut-brain axis. In this study, we aimed to determine the protective effect of Bifidobacterium bifidum BGN4 (B. bifidum BGN4) and Bifidobacterium longum BORI (B. longum BORI) on age-related brain damage in mice. We found that administration of B. bifidum BGN4 and B. longum BORI effectively elevates brain-derived neurotrophic factor expression which was mediated by increased histone 3 lysine 9 trimethylation. Furthermore, administration of probiotic supplementation reversed the DNA damage and apoptotic response in aged mice and also improved the age-related cognitive and memory deficits of these mice. Taken together, the present study highlights the anti-aging effects of B. bifidum BGN4 and B. longum BORI in the aged brain and their beneficial effects for age-related brain disorders.


Assuntos
Bifidobacterium bifidum , Bifidobacterium longum , Microbioma Gastrointestinal , Probióticos , Animais , Bifidobacterium bifidum/genética , Camundongos , Rejuvenescimento
16.
Aging (Albany NY) ; 14(6): 2507-2512, 2022 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-35314518

RESUMO

Heterochronic parabiosis is used to study the systemic effects of aging and involves surgically connecting two animals of different ages such that they have common blood circulation. Although this technique has been prevalent for a long time, there is no scientific consensus on the age of the animals that should be used. We hypothesized that the younger the animal, the greater would be its rejuvenating effect. Hence, to test this hypothesis, we created parabiosis of 67-week-old mice with younger mice of different ages (4-week-old and 8-week-old). We evaluated the changes in appearance and the expression IL-1A, IL-6, and Cdkn2a (p16) in the liver, kidney, brain, and skin. These cytokines belong to the senescence-associated secretory phenotype (SASP) factors, and are indicators of aging. Although we did not find any significant changes in the appearance of the mice, we found statistically significant differences in some SASP factors between the liver of the 4-week-old and 8-week-old pairs. However, overall, compared to the 8-week-old mice, the 4-week-old does not exert a significantly higher rejuvenation effect on the older mice. Hence, we concluded that the rejuvenation of older mice during heterochronic parabiosis might not be affected by the exact age of the younger mice.


Assuntos
Parabiose , Rejuvenescimento , Envelhecimento , Animais , Proteínas Inibidoras de Quinase Dependente de Ciclina , Citocinas , Camundongos
17.
Proc Biol Sci ; 289(1970): 20212434, 2022 03 09.
Artigo em Inglês | MEDLINE | ID: mdl-35232226

RESUMO

Ageing, death, and potential immortality lie at the heart of biology, but two seemingly incompatible paradigms coexist in different research communities and have done since the nineteenth century. The universal senescence paradigm sees senescence as inevitable in all cells. Damage accumulates. The potential immortality paradigm sees some cells as potentially immortal, especially unicellular organisms, germ cells and cancerous cells. Recent research with animal cells, yeasts and bacteria show that damaged cell constituents do in fact build up, but can be diluted by growth and cell division, especially by asymmetric cell division. By contrast, mammalian embryonic stem cells and many cancerous and 'immortalized' cell lines divide symmetrically, and yet replicate indefinitely. How do they acquire their potential immortality? I suggest they are rejuvenated by excreting damaged cell constituents in extracellular vesicles. If so, our understanding of cellular senescence, rejuvenation and potential immortality could be brought together in a new synthesis, which I call the cellular rejuvenation hypothesis: damaged cell constituents build up in all cells, but cells can be rejuvenated either by growth and cell division or, in 'immortal' cell lines, by excreting damaged cell constituents. In electronic supplementary material, appendix, I outline nine ways in which this hypothesis could be tested.


Assuntos
Senescência Celular , Rejuvenescimento , Envelhecimento , Animais , Divisão Celular , Mamíferos
18.
Aging Cell ; 21(4): e13577, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35266272

RESUMO

Rejuvenation of nucleus pulposus cells (NPCs) in degenerative discs can reverse intervertebral disc degeneration (IDD). Partial reprogramming is used to rejuvenate aging cells and ameliorate progression of aging tissue to avoiding formation of tumors by classical reprogramming. Understanding the effects and potential mechanisms of partial reprogramming in degenerative discs provides insights for development of new therapies for IDD treatment. The findings of the present study show that partial reprogramming through short-term cyclic expression of Oct-3/4, Sox2, Klf4, and c-Myc (OSKM) inhibits progression of IDD, and significantly reduces senescence related phenotypes in aging NPCs. Mechanistically, short-term induction of OSKM in aging NPCs activates energy metabolism as a "energy switch" by upregulating expression of Hexokinase 2 (HK2) ultimately promoting redistribution of cytoskeleton and restoring the aging state in aging NPCs. These findings indicate that partial reprogramming through short-term induction of OSKM has high therapeutic potential in the treatment of IDD.


Assuntos
Degeneração do Disco Intervertebral , Disco Intervertebral , Núcleo Pulposo , Reprogramação Celular , Humanos , Disco Intervertebral/metabolismo , Degeneração do Disco Intervertebral/metabolismo , Núcleo Pulposo/metabolismo , Rejuvenescimento
19.
Aging Cell ; 21(3): e13578, 2022 03.
Artigo em Inglês | MEDLINE | ID: mdl-35235716

RESUMO

The expression of the pluripotency factors OCT4, SOX2, KLF4, and MYC (OSKM) can convert somatic differentiated cells into pluripotent stem cells in a process known as reprogramming. Notably, partial and reversible reprogramming does not change cell identity but can reverse markers of aging in cells, improve the capacity of aged mice to repair tissue injuries, and extend longevity in progeroid mice. However, little is known about the mechanisms involved. Here, we have studied changes in the DNA methylome, transcriptome, and metabolome in naturally aged mice subject to a single period of transient OSKM expression. We found that this is sufficient to reverse DNA methylation changes that occur upon aging in the pancreas, liver, spleen, and blood. Similarly, we observed reversion of transcriptional changes, especially regarding biological processes known to change during aging. Finally, some serum metabolites and biomarkers altered with aging were also restored to young levels upon transient reprogramming. These observations indicate that a single period of OSKM expression can drive epigenetic, transcriptomic, and metabolomic changes toward a younger configuration in multiple tissues and in the serum.


Assuntos
Reprogramação Celular , Células-Tronco Pluripotentes Induzidas , Animais , Diferenciação Celular , Reprogramação Celular/genética , Metilação de DNA/genética , Epigenoma , Células-Tronco Pluripotentes Induzidas/metabolismo , Camundongos , Rejuvenescimento
20.
Cells ; 11(5)2022 02 28.
Artigo em Inglês | MEDLINE | ID: mdl-35269453

RESUMO

In this review, we seek a novel strategy for establishing a rejuvenating microenvironment through senescent cells specific reprogramming. We suggest that partial reprogramming can produce a secretory phenotype that facilitates cellular rejuvenation. This strategy is desired for specific partial reprogramming under control to avoid tumour risk and organ failure due to loss of cellular identity. It also alleviates the chronic inflammatory state associated with ageing and secondary senescence in adjacent cells by improving the senescence-associated secretory phenotype. This manuscript also hopes to explore whether intervening in cellular senescence can improve ageing and promote damage repair, in general, to increase people's healthy lifespan and reduce frailty. Feasible and safe clinical translational protocols are critical in rejuvenation by controlled reprogramming advances. This review discusses the limitations and controversies of these advances' application (while organizing the manuscript according to potential clinical translation schemes) to explore directions and hypotheses that have translational value for subsequent research.


Assuntos
Envelhecimento , Reprogramação Celular , Envelhecimento/patologia , Senescência Celular/genética , Humanos , Longevidade , Rejuvenescimento
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