Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 781
Filtrar
1.
Phytochemistry ; 172: 112285, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32035325

RESUMO

Plant glandular trichomes (GTs) are adaptive epidermal structures that synthesize and accumulate diverse specialized metabolites well-known as defense chemicals against biotic attacks, but their roles against abiotic challenges including UV radiation and cold climates remain largely unexplored. Colquhounia vestita Wall is a Chinese-Himalayan Lamiaceae plant with dense peltate and capitate GTs on its leaf and stem surfaces under a scanning electron microscope. Three diterpenoid acids, including a clerodane 5-epi-hardwickiic acid and two labdanes polyalthic acid and E-communic acid, were identified from the peltate GTs of C. vestita through laser microdissection coupled with UPLC-MS/MS. Under UV radiation and cold stresses, the major GT component polyalthic acid increased the biomass of Arabidopsis thaliana seedlings and decreased their malondialdehyde content. Furthermore, polyalthic acid promoted photosynthetic efficiency and the expression of genes encoding peroxidative enzymes under UV radiation, and stimulated Ca2+ elevation and the expression of calmodulin binding transcription activator gene CAMTA3 and two downstream cold-responsive genes CBF3 and RD29A under cold stress. Therefore, polyalthic acid in GTs is likely to endow the plant with enhanced tolerance to UV radiation and cold stresses, which extends the current understanding of the function of GT compounds in plant adaptation to abiotic environments.


Assuntos
Lamiaceae , Tricomas , Cromatografia Líquida , Resposta ao Choque Frio , Regulação da Expressão Gênica de Plantas , Espectrometria de Massas em Tandem , Raios Ultravioleta
2.
Life Sci ; 246: 117432, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32061867

RESUMO

Our previous studies have shown that prenatal cold stress leads to placental inflammatory response and induces anxiety-like behavior reduced in offspring rats. However, the role and mechanisms by which prenatal cold stress affects offspring remain unclear. The aim of this study was to determine the metabolic profiles from the maternal serum and helpful in understanding the role and mechanisms by which prenatal cold stress affects the offspring. In this study, liquid chromatography-mass spectrometry (LC/MS) was used to analyze serum metabolites, and PCA, PLS-DA, and OPLS-DA were performed to analyze changes in metabolites in the maternal serum after cold stress of 3 or 7 days. The results showed that 19 metabolites in the CS (cold stress 7 days)-NS (control) group and 23 metabolites in the CT (cold stress 3 days)-NT (control) group were significantly altered. These metabolites were mainly associated with unsaturated fatty acid synthesis, and arachidonic acid, linoleic acid, and glutamine and glutamate metabolism. The data indicated that prenatal cold stress not only affected the maternal neuroendocrine system, but also affected the immune system, and lipid and amino acid metabolism. These results further supported the findings of our previous studies on the effects of prenatal cold stress on the mother and offspring. A more comprehensive understanding of these data may lead to maternal intervention that can reverse the damage of prenatal stressors.


Assuntos
Resposta ao Choque Frio , Metabolômica , Efeitos Tardios da Exposição Pré-Natal/etiologia , Animais , Feminino , Cromatografia Gasosa-Espectrometria de Massas , Masculino , Gravidez , Efeitos Tardios da Exposição Pré-Natal/sangue , Efeitos Tardios da Exposição Pré-Natal/metabolismo , Ratos , Ratos Wistar
3.
Plant Mol Biol ; 102(4-5): 447-462, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31898148

RESUMO

KEY MESSAGE: ZjICE2 works as a positive regulator in abiotic stress responses and ZjICE2 is a valuable genetic resource to improve abiotic stress tolerance in the molecular breeding program of Zoysia japonica. The basic helix-loop-helix (bHLH) family transcription factors (TFs) play an important role in response to biotic or abiotic stresses in plants. However, the functions of bHLH TFs in Zoysia japonica, one of the warm-season turfgrasses, remain poorly understood. Here, we identified ZjICE2 from Z. japonica, a novel MYC-type bHLH transcription factor that was closely related to ICE homologs in the phylogenetic tree, and its expression was regulated by various abiotic stresses. Transient expression of ZjICE2-GFP in onion epidermal cells revealed that ZjICE2 was a nuclear-localized protein. Also, ZjICE2 bound the MYC cis-element in the promoter of dehydration responsive element binding 1 of Z. japonica (ZjDREB1) using yeast one-hybrid assay. A phenotypic analysis showed that overexpression of the ZjICE2 in Arabidopsis enhanced tolerance to cold, drought, and salt stresses. The transgenic Arabidopsis and Z. japonica accumulated more transcripts of cold-responsive DREB/CBFs and their downstream genes than the wild type (WT) after cold treatment. Furthermore, the transgenic plants exhibited an enhanced Reactive oxygen species (ROS) scavenging ability, which resulted in an efficient maintenance of oxidant-antioxidant homeostasis. In addition, overexpression of the ZjICE2 in Z. japonica displayed intensive cold tolerance with increases in chlorophyll contents and photosynthetic efficiency. Our study suggests that ZjICE2 works as a positive regulator in abiotic stress responses and the ICE-DREB/CBFs response pathway involved in cold stress tolerance is also conserved in Z. japonica. These results provide a valuable genetic resource for the molecular breeding program especially for warm-season grasses as well as other leaf crop plants.


Assuntos
Fatores de Transcrição Hélice-Alça-Hélice Básicos/fisiologia , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/fisiologia , Poaceae/fisiologia , Espécies Reativas de Oxigênio/metabolismo , Estresse Fisiológico , Arabidopsis/genética , Arabidopsis/fisiologia , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/fisiologia , Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Temperatura Baixa , Resposta ao Choque Frio , Secas , Filogenia , Proteínas de Plantas/genética , Plantas Geneticamente Modificadas/fisiologia , Poaceae/genética , Regulon , Tolerância ao Sal , Fatores de Transcrição/genética , Fatores de Transcrição/fisiologia , Ativação Transcricional
4.
Gene ; 727: 144244, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31715303

RESUMO

Sweetpotato (Ipomoea batatas [L.] Lam) is considered an economically important crop worldwide and is used as a source of food, feed, and biomaterials. However, its origin in tropical regions makes it vulnerable to chilling injury during postharvest storage at low temperature. To gain further insight into the molecular mechanism of chilling response, we performed comparative transcriptome analysis of two sweetpotato lines, Xushu 15-1 and Xushu 15-4, with high and low cold storage ability, respectively. Tuberous roots of these lines were stored at 4 °C for 0, 2, and 6 weeks. RNA-Seq data of both lines were de novo assembled, producing 27,636 unigenes with a N50 value of 1204 bp. A total of 525 differentially expressed genes (DEGs) were identified and categorized into six clusters. Genes with higher expression in Xushu 15-1 than in Xushu 15-4 significantly increased in number over time during low temperature storage. Functional annotation of DEGs using KEGG enrichment analysis showed that these DEGs were involved in carbohydrate metabolism, ribosome, protein processing in endoplasmic reticulum, plant-pathogen interaction, and plant hormone signal transduction. Several key candidate genes involved in KEGG pathways were selected and discussed further. The results of this study enhance our understanding of the complex mechanisms involved in low temperature tolerance in sweetpotato during storage and provide a set of candidate genes for the development of new varieties with improved cold storage ability.


Assuntos
Resposta ao Choque Frio/genética , Ipomoea batatas/genética , Temperatura Baixa/efeitos adversos , Perfilação da Expressão Gênica/métodos , Regulação da Expressão Gênica de Plantas/genética , Anotação de Sequência Molecular/métodos , Raízes de Plantas/genética , Análise de Sequência de RNA/métodos , Temperatura Ambiente , Transcriptoma/genética
5.
Insect Sci ; 27(2): 317-335, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30381878

RESUMO

Drosophila suzukii is an invasive pest causing severe damages to a large panel of cultivated crops. To facilitate its biocontrol with strategies such as sterile or incompatible insect techniques, D. suzukii must be mass-produced and then stored and transported under low temperature. Prolonged cold exposure induces chill injuries that can be mitigated if the cold period is interrupted with short warming intervals, referred to as fluctuating thermal regimes (FTR). In this study, we tested how to optimally use FTR to extend the shelf life of D. suzukii under cold storage. Several FTR parameters were assessed: temperature (15, 20, 25 °C), duration (0.5, 1, 2, 3 h), and frequency (every 12, 24, 36, 48 h) of warming intervals, in two wild-type lines and in two developmental stages (pupae and adults). Generally, FTR improved cold storage tolerance with respect to constant low temperatures (CLT). Cold mortality was lower when recovery temperature was 20 °C or higher, when duration was 2 h per day or longer, and when warming interruptions occurred frequently (every 12 or 24 h). Applying an optimized FTR protocol to adults greatly reduced cold mortality over long-term storage (up to 130 d). Consequences of FTR on fitness-related traits were also investigated. For adults, poststorage survival was unaffected by FTR, as was the case for female fecundity and male mating capacity. On the other hand, when cold storage occurred at pupal stage, poststorage survival and male mating capacity were altered under CLT, but not under FTR. After storage of pupae, female fecundity was lower under FTR compared to CLT, suggesting an energy trade-off between repair of chill damages and egg production. This study provides detailed information on the application and optimization of an FTR-based protocol for cold storage of D. suzukii that could be useful for the biocontrol of this pest.


Assuntos
Resposta ao Choque Frio , Drosophila/fisiologia , Traços de História de Vida , Animais , Feminino , Fertilidade , Masculino , Comportamento Sexual Animal
6.
Int J Food Microbiol ; 312: 108362, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31669764

RESUMO

Stuck and sluggish fermentations are among the main problems in winemaking industry leading to important economic losses. Several factors have been described as causes of stuck and sluggish fermentations, being exposure to extreme temperatures barely studied. The objective of this study was to identify thermal conditions leading to stuck and sluggish fermentations, focusing on the impact of an abrupt and transient decrease/increase of temperature on fermentation performance and yeast viability/vitality. Different strains of Saccharomyces cerevisiae, SBB11, T73, and PDM were evaluated in synthetic grape must fermentations. Cold shocks (9 °C and 1.5 °C for 16 h) carried out on different days during the fermentation process were unable to alter fermentation performance. Conversely, shock temperatures higher than 32 °C, applied in early stages of the process, lead to sluggish fermentation showing a delay directly related to the temperature increase. Fermentation delay was associated with a decrease in cell vitality. The impact of the heat shock on fermentation performance was different depending on the strain evaluated and nitrogen supplementation (with or without diammonium phosphate addition). None of the conditions evaluated produced a stuck fermentation and importantly, in all cases must nutrition improved fermentation performance after a heat shock.


Assuntos
Temperatura Baixa , Fermentação/fisiologia , Temperatura Alta , Saccharomyces cerevisiae/metabolismo , Resposta ao Choque Frio/fisiologia , Resposta ao Choque Térmico/fisiologia , Fosfatos/farmacologia , Vitis/metabolismo , Vinho/análise
7.
Plant Biol (Stuttg) ; 22 Suppl 1: 133-142, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30597710

RESUMO

Among wild species used in potato breeding, Solanum commersonii displays the highest tolerance to low temperatures under both acclimated (ACC) and non-acclimated (NACC) conditions. It is also the first wild potato relative with a known whole genome sequence. Recent studies have shown that abiotic stresses induce changes in the expression of many small non-coding RNA (sncRNA). We determined the small non-coding RNA (sncRNAome) of two clones of S. commersonii contrasting in their cold response phenotypes via smRNAseq. Differential analysis provided evidence that expression of several miRNAs changed in response to cold stress conditions. Conserved miR408a and miR408b changed their expression under NACC conditions, whereas miR156 and miR169 were differentially expressed only under ACC conditions. We also report changes in tasiRNA and secondary siRNA expression under both stress conditions. Our results reveal possible roles of sncRNA in the regulatory networks associated with tolerance to low temperatures and provide useful information for a more strategic use of genomic resources in potato breeding.


Assuntos
Resposta ao Choque Frio , MicroRNAs , Solanum tuberosum , Solanum , Temperatura Baixa , Regulação da Expressão Gênica de Plantas , MicroRNAs/genética , RNA/genética , Bibliotecas de Moléculas Pequenas , Solanum/genética
8.
J Insect Sci ; 19(6)2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31752020

RESUMO

Galeruca daurica (Joannis) has become a new insect pest in the Inner Mongolia grasslands since 2009, and its larvae and eggs have strong cold tolerance. To get a deeper insight into its molecular mechanisms of cold stress responses, we performed de novo transcriptome assembly for G. daurica by RNA-Seq and compared the transcriptomes of its larvae exposed to five different temperature treatments (-10, -5, 0, 5, and 25°C for 1 h and then recovered at 25°C for 1 h), respectively. Compared with the control (25°C), the numbers of differentially expressed genes (DEGs) decreased from 1,821 to 882, with the temperature declining from 5 to -10°C. Moreover, we obtained 323 coregulated DEGs under different low temperatures. Under four low temperatures (-10, -5, 0, and 5°C), a large number of genes were commonly upregulated during recovery from cold stresses, including those related to cuticle protein, followed by cytochrome P450, clock protein, fatty acid synthase, and fatty acyl-CoA reductase; meanwhile, lots of genes encoding cuticle protein, RNA replication protein, RNA-directed DNA polymerase, and glucose dehydrogenase were commonly downregulated. Our findings provide important clues for further investigations of key genes and molecular mechanisms involved in the adaptation of G. daurica to harsh environments.


Assuntos
Resposta ao Choque Frio , Besouros/metabolismo , Transcriptoma , Animais , Besouros/genética , Regulação da Expressão Gênica
9.
C R Biol ; 342(9-10): 299-308, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31734081

RESUMO

The Heat Shock Proteins (HSPs) are a superfamily of molecular chaperones that maintain cellular homeostasis under stress. HSP70 represents the major stress-inducible family member, often activated in response to changes in thermal ranges of organisms, and therefore playing an important role enhancing thermal tolerance limits in ectothermic animals. The present study aimed to investigate the presence and the localization of HSP70 through the development of Podarcis siculus, an oviparous lizard inhabiting temperate Mediterranean regions, showing a limited potential to tolerate thermal changes during embryogenesis. Immunohistochemical analysis demonstrated that HSP70 protein is constitutively present in early embryonic stages, abundantly distributed in eye, in encephalic domains (predominantly in ventricular areas and in grey matter), in grey matter of spinal cord, in lung, gut mucosa, hepatic cords and kidney tubules. Interestingly, a severe drop in incubation temperature (5°C for 3 days) does not induce enhancements in HSP70 levels nor changes in tissues localization. These results suggest that the HSP70 found in P. siculus embryos represents a non-inducible, constitutive molecular chaperone that should be better called Heat Shock Cognate 70 (HSC70); the presence of stress-induced members of the HSP family in P. siculus has yet to be proven.


Assuntos
Resposta ao Choque Frio/fisiologia , Proteínas de Choque Térmico HSP70/metabolismo , Lagartos/fisiologia , Animais , Desenvolvimento Embrionário/fisiologia , Temperatura Alta , Temperatura Ambiente
10.
Sci Total Environ ; 688: 970-975, 2019 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-31726579

RESUMO

BACKGROUND: Hypertension (HT) affects >1/3 of adult populations in developed countries. Several studies reported periodic changes in blood pressure (BP) values depending on atmospheric conditions, and analyzed the impact of outdoor temperature, atmospheric pressure, relative humidity and other meteorological parameters. The Universal Thermal Climate Index (UTCI) is a bio-meteorological index derived from an analysis of human thermal balance that comprehensively describes the impact of meteorological factors, including both cold stress and heat stress and their physiological consequences. AIM: Our aim was to assess the relationship between UTCI values and the number of consultations for HT within the framework of the healthcare system. METHODS: This work presents a retrospective epidemiological study of data collected in Olsztyn (Poland), characterized by cold climate type. The analytical material comprised 5578 consultations in emergency departments (EDs) due to HT. RESULTS: Seasonal differences in the numbers of HT consultations with a significant increase in winter months were noticed, especially as regards women. Under cold stress conditions, the relative risk (RR) related to consultations for HT was 2-fold higher for women as compared to thermoneutral conditions (p < 0.001). For men these differences were also statistically significant, though at a much smaller level (p = 0.03). The increased RR of HT due to cold stress was found among younger and older women contrary to the women at the perimenopausal age. CONCLUSIONS: Cold stress should be considered as a significant risk factor among patients, particularly women, diagnosed with cardiovascular diseases. The UTCI is an adequate bio-meteorological tool for the assessment of relationships between atmospheric conditions and occurrence of cardiac symptoms.


Assuntos
Resposta ao Choque Frio , Exposição Ambiental/estatística & dados numéricos , Hipertensão/epidemiologia , Adulto , Pressão Sanguínea , Doenças Cardiovasculares , Clima , Humanos , Conceitos Meteorológicos , Polônia/epidemiologia
12.
Lima; Perú. Ministerio de Salud; 3 ed; 20191100. 11 p. ilus.
Monografia em Espanhol | LILACS, LIPECS | ID: biblio-1026275

RESUMO

La cartilla informativa está dirigida a la comunidad educativa para que se desarrollen prácticas saludables en el ámbito escolar, frente a la temporada de bajas temperaturas, para reducir las consecuencias de este evento climatológico sobre la salud de las personas, fundamentalmente las de mayor vulnerabilidad.


Assuntos
Medição de Risco , Desastre Meteorológico , Planos e Programas de Educação em Desastres , Geada , Exposição Ambiental , Resposta ao Choque Frio , Promoção da Saúde
13.
Lima; Perú. Ministerio de Salud; 3 ed; 20191100. 11 p. ilus.
Monografia em Espanhol | LILACS, LIPECS | ID: biblio-1026277

RESUMO

La cartilla informativa es para los gobiernos locales que fortalezcan los conocimientos de su comunidad para que desarrollen prácticas saludables frente a esta temporada de bajas temperaturas, de manera que puedan organizar la vigilancia comunal de su población vulnerable y posibilite la reducción del efecto negativo de las heladas en la salud de la población.


Assuntos
Planos e Programas de Saúde , Medição de Risco , Desastre Meteorológico , Geada , Exposição Ambiental , Resposta ao Choque Frio , Promoção da Saúde
14.
Int J Mol Sci ; 20(20)2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31615063

RESUMO

Chilling stress is considered the major abiotic stress affecting the growth, development, and yield of rice. To understand the transcriptomic responses and methylation regulation of rice in response to chilling stress, we analyzed a cold-tolerant variety of rice (Oryza sativa L. cv. P427). The physiological properties, transcriptome, and methylation of cold-tolerant P427 seedlings under low-temperature stress (2-3 °C) were investigated. We found that P427 exhibited enhanced tolerance to low temperature, likely via increasing antioxidant enzyme activity and promoting the accumulation of abscisic acid (ABA). The Methylated DNA Immunoprecipitation Sequencing (MeDIP-seq) data showed that the number of methylation-altered genes was highest in P427 (5496) and slightly lower in Nipponbare (Nip) and 9311 (4528 and 3341, respectively), and only 2.7% (292) of methylation genes were detected as common differentially methylated genes (DMGs) related to cold tolerance in the three varieties. Transcriptome analyses revealed that 1654 genes had specifically altered expression in P427 under cold stress. These genes mainly belonged to transcription factor families, such as Myeloblastosis (MYB), APETALA2/ethylene-responsive element binding proteins (AP2-EREBP), NAM-ATAF-CUC (NAC) and WRKY. Fifty-one genes showed simultaneous methylation and expression level changes. Quantitative RT-PCR (qRT-PCR) results showed that genes involved in the ICE (inducer of CBF expression)-CBF (C-repeat binding factor)-COR (cold-regulated) pathway were highly expressed under cold stress, including the WRKY genes. The homologous gene Os03g0610900 of the open stomatal 1 (OST1) in rice was obtained by evolutionary tree analysis. Methylation in Os03g0610900 gene promoter region decreased, and the expression level of Os03g0610900 increased, suggesting that cold stress may lead to demethylation and increased gene expression of Os03g0610900. The ICE-CBF-COR pathway plays a vital role in the cold tolerance of the rice cultivar P427. Overall, this study demonstrates the differences in methylation and gene expression levels of P427 in response to low-temperature stress, providing a foundation for further investigations of the relationship between environmental stress, DNA methylation, and gene expression in rice.


Assuntos
Resposta ao Choque Frio/genética , Oryza/genética , Plântula/genética , Transcrição Genética , Regulação da Expressão Gênica de Plantas/genética , Metilação , Oryza/crescimento & desenvolvimento , Proteínas Quinases/genética , Plântula/crescimento & desenvolvimento , Transcriptoma/genética
15.
BMC Genomics ; 20(1): 740, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31615400

RESUMO

BACKGROUND: Chinese wild grapevine (Vitis amurensis) has remarkable cold stress tolerance, exceeding that of the common cultivated grapevine (Vitis vinifera L.). RESULT: Here, we surveyed the expression dynamics of microRNAs (miRNAs) across Chinese wild grapevine (cv. Beibinghong) and cultivated grapevine (cv. Cabernet Sauvignon) under cold stress using high-throughput sequencing. We identified 186 known miRNAs in cultivated grape and 427 known miRNAs in Beibinghong. Of the identified miRNAs, 59 are conserved miRNAs orthologous in Cabernet Sauvignon and Beibinghong. In addition, 105 and 129 novel miRNAs were identified in Cabernet Sauvignon and Beibinghong, respectively. The expression of some miRNAs was related to cold stress both in Cabernet Sauvignon and Beibinghong. Many cold-related miRNAs in Cabernet Sauvignon and Beibinghong were predicted to target stress response-related genes such as MYB, WRKY, bHLH transcription factor genes, and heat shock protein genes. However, the expression tendency under cold treatment of many of these miRNAs was different between Cabernet Sauvignon and Beibinghong. Different modes of expression of cultivated and Chinese wild grape miRNAs were indicated in key pathways under cold stress by degradome, target prediction, GO, and KEGG analyses. CONCLUSION: Our study indicated three likely reasons that led to the different cold stress tolerance levels of Cabernet Sauvignon and Beibinghong. Specifically, there may be (1) differential expression of orthologous miRNAs between cultivated grapevine and Chinese wild grape; (2) species-specific miRNAs or target genes; or (3) different regulatory models of miRNAs in cultivated and Chinese wild grape in some key pathways.


Assuntos
Redes Reguladoras de Genes , Sequenciamento de Nucleotídeos em Larga Escala/métodos , MicroRNAs/genética , Vitis/crescimento & desenvolvimento , China , Resposta ao Choque Frio , Produtos Agrícolas/genética , Produtos Agrícolas/crescimento & desenvolvimento , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , RNA de Plantas/genética , Análise de Sequência de RNA , Vitis/genética
16.
Plant Sci ; 289: 110254, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31623785

RESUMO

ICE1 (Inducer of CBF Expression 1) is a regulator of cold-induced transcriptome, which plays an important role in plant cold response pathway. To enhance the cold tolerance of Zoysia japonica, one of the warm-season turfgrasses, it is helpful to understand the cold response mechanism in Zoysia japonica. We identified stress-responsive ZjICE1 from Zoysia japonica and characterized its function in cold stress. Our results showed that ZjICE1 shared the typical feature of ICE homolog proteins belonging to a nucleic protein. Transactivation activity assay revealed that ZjICE1 bound to the MYC cis-element in the ZjDREB1's promotor. The ZjICE1 overexpressed transgenic Arabidopsis showed enhanced tolerance to cold stress with an increases in SOD, POD, and free proline content and reduction in MDA content. They also induced the transcripts abundance of cold-responsive genes (CBF1, CBF2, CBF3, COR47A, KIN1, and RD29A) after cold treatment. These results suggest that ZjICE1 is a positive regulator in Zoysia japonica plant during cold stress and can be a useful gene for the molecular breeding program to develop the cold tolerant zoysiagrass. Furthermore, the ZjICE1 also conferred resistance to salt and drought stresses, providing the better understanding of the basic helix-loop-helix (bHLH) gene family in abiotic stress responses.


Assuntos
Aclimatação/genética , Arabidopsis/fisiologia , Resposta ao Choque Frio/genética , Proteínas de Plantas/genética , Poaceae/fisiologia , Fatores de Transcrição/genética , Sequência de Aminoácidos , Arabidopsis/genética , Temperatura Baixa , Filogenia , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/fisiologia , Poaceae/genética , Alinhamento de Sequência , Fatores de Transcrição/química , Fatores de Transcrição/metabolismo
18.
Lima; Perú. Ministerio de Salud; 3 ed; 20191000. 11 p. ilus.
Monografia em Espanhol | LILACS, LIPECS | ID: biblio-1026266

RESUMO

El documento contiene prácticas saludables frente a las temporadas de bajas temperaturas para que la comunidad educativa fortalezcan sus labores en la promoción de hábitos, comportamiento y entornos saludables en el ámbito escolar.


Assuntos
Clima Frio , Medição de Risco , Planos e Programas de Educação em Desastres , Exposição Ambiental , Materiais Educativos e de Divulgação , Resposta ao Choque Frio , Promoção da Saúde
19.
Lima; Perú. Ministerio de Salud; 3 ed; 20191000. 14 p. ilus.
Monografia em Espanhol | LILACS, LIPECS | ID: biblio-1026272

RESUMO

El documento contiene prácticas saludables frente a las temporadas de bajas temperaturas para que los gobiernos locales fortalezcan los conocimientos de su comunidad para que desarrollen prácticas saludables frente a esta temporada de bajas temperaturas, de manera que puedan organizar la vigilancia comunal de su población vulnerable y posibilite la reducción del efecto negativo del friaje en la salud de la población.


Assuntos
Infecções Respiratórias , Clima Frio , Medição de Risco , Desastre Meteorológico , Planos e Programas de Educação em Desastres , Exposição Ambiental , Resposta ao Choque Frio , Promoção da Saúde
20.
DNA Cell Biol ; 38(11): 1233-1248, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31532241

RESUMO

Verbena bonariensis is a valuable plant for both ornament and flower border. As a major constraint, low temperature affects the growing development and survival of V. bonariensis. However, there are few systematic studies in terms of molecular mechanism on the tolerance of low temperature in V. bonariensis. In this study, Illumina sequencing technology was applied to analyze the cold resistance mechanism of plants. Six cDNA libraries were obtained from two samples of two groups, the cold-treated group and the control group. A total of 271,920 unigenes were produced from 406,641 assembled transcripts. Among these, 19,003 differentially expressed genes (DEGs) (corrected p-value <0.01, |log2(fold change) | >3) were obtained, including 9852 upregulated and 9151 downregulated genes. The antioxidant enzyme system, photosynthesis, plant hormone signal transduction, fatty acid metabolism, starch and sucrose metabolism pathway, and transcription factors were analyzed. Based on these results, series of candidate genes related to cold stress were screened out and discussed. The physiological indexes related to response mechanism of low temperature were tested. Eleven upregulated DEGs were validated by Quantitative Real-time PCR. In this study, we provided the transcriptome sequence resource of V. bonariensis and used these data to realize its molecular mechanism under cold stress. The results contributed to valuable clues for genetic studies and helped to screen candidate genes for cold-resistance breeding.


Assuntos
Resposta ao Choque Frio/genética , Folhas de Planta/genética , Proteínas de Plantas/genética , Transcriptoma , Verbena/fisiologia , Temperatura Baixa , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Sequenciamento de Nucleotídeos em Larga Escala , Folhas de Planta/metabolismo , Proteínas de Plantas/análise , Estresse Fisiológico/genética , Temperatura Ambiente , Verbena/genética , Verbena/crescimento & desenvolvimento
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA