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1.
Food Microbiol ; 83: 211-218, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31202415

RESUMO

The processing conditions and physiochemical properties used in food manufacturing create niches which support the growth of a limited number of spoilage fungi. This study was designed to evaluate the influence of intrinsic and extrinsic food product variables on the identity of spoilage fungi genera isolated from commercially produced foods. The spoilage etiology was identified in 127 products through ITS region sequencing. The prevalence and diversity of the identified spoilage fungi were evaluated in relationship to product-specific attributes using various descriptive statistics and a bipartite network analysis. Additionally, recursive partitioning was used to generate a classification tree with the outcomes, genera of the spoilage isolates, divided into increasingly homogenous subgroups. All of the isolated fungi belonged to the Ascomycete phylum, except four mucoralian isolates and the basidiomycete Rhodotorula. The occurrence of filamentous fungi repeatedly isolated ranged from 2% (Phoma spp.) to 18% (Penicillium spp.). In order of decreasing contribution to subgroup homogeneity, the split rules for the classification tree were based on process, water activity, food matrix category, and pH. Fungal genera representation in the terminal nodes indicated that production failures, in addition to product-specific attributes, were responsible for determination of the most probable spoilage organism.


Assuntos
Fast Foods/análise , Fast Foods/microbiologia , Contaminação de Alimentos/análise , Manipulação de Alimentos , Fungos/isolamento & purificação , Microbiologia de Alimentos , Fungos/classificação , Penicillium/isolamento & purificação , Rhodotorula/isolamento & purificação
2.
Arch Microbiol ; 201(3): 399-407, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30719527

RESUMO

During this study, we characterized the seasonality's impact and environmental conditions on the yeast diversity from raw camel's milk collected in Algeria. The yeast counts were estimated to 3.55 × 102 CFU mL-1, with a maximum of 6.3 × 102 CFU mL-1. The yeasts were categorized phenotypically by API 20C AUX, MALDI-TOF and genetically by sequencing 26S rDNA and ITS1-5.8S-ITS2. The rDNA sequencing approaches revealed 12 species including unusual ones such as Trichosporon asahii, Pichia fermentans, Millerozyma farinosa, Pichia galeiformis, Candida tartarivorans and Pichia manshurica. The most dominant species were T. asahii (23%), P. fermentans (19%) and Rhodotorula mucilaginosa (14%). The high occurrence and large diversity were registered in samples collected during the autumn season, in the semi-arid and arid highlands regions with 0.66 × 103 CFU mL-1 and 0.51 × 103 CFU mL-1, respectively. Interestingly, T. asahii, R. mucilaginosa, P. fermentans, C. parapsilosis and C. zeylanoides were detected during both spring and autumn.


Assuntos
Camelus/microbiologia , Candida/isolamento & purificação , Leite/microbiologia , Pichia/isolamento & purificação , Rhodotorula/isolamento & purificação , Saccharomyces cerevisiae/isolamento & purificação , Leveduras/isolamento & purificação , Argélia , Animais , Candida/classificação , Candida/genética , DNA Fúngico/genética , DNA Ribossômico/genética , Pichia/classificação , Pichia/genética , Rhodotorula/classificação , Rhodotorula/genética , Saccharomyces cerevisiae/classificação , Saccharomyces cerevisiae/genética , Estações do Ano , Leveduras/classificação , Leveduras/genética
3.
Mycoses ; 62(2): 90-100, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30267601

RESUMO

BACKGROUND: Fungal infections by Rhodotorula species are increasingly reported in the literature and consist of bloodstream infections, especially in patients with central venous catheters (CVC), as well as central nervous system (CNS), ocular and other less frequent infections. OBJECTIVES: The aim of this systematic review was to record and evaluate all available evidence regarding infections by Rhodotorula species. METHODS: Systematic review of PubMed (through 9 August 2017) for studies providing epidemiological, clinical, microbiological, as well as treatment data and outcomes of Rhodotorula species infections. RESULTS: A total of 111 studies, containing data of 248 patients, were eventually included in the analysis. The most common Rhodotorula infections were those of bloodstream, CNS and ocular infections, as well as peritoneal dialysis-associated peritonitis. Epidemiology of each type of infection was different, with the bloodstream ones being more common in patients with malignancy and CVCs, while those of the CNS were more common in patients with AIDS. Mortality was variable being higher in CNS infections. Amphotericin B remains the most common agent used for treatment, irrespectively of the infection site. CONCLUSIONS: This systematic review thoroughly describes fungal infections by Rhodotorula species and provides information on their epidemiology, clinical picture, microbiology, treatment and outcomes.


Assuntos
Antifúngicos/uso terapêutico , Micoses/epidemiologia , Micoses/patologia , Rhodotorula/isolamento & purificação , Humanos , Micoses/tratamento farmacológico , Micoses/microbiologia , Resultado do Tratamento
4.
Mycoses ; 62(3): 223-229, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30525252

RESUMO

BACKGROUND: Rhodotorula spp are uncommon yeasts able to cause infections with high mortality rates. Rhodotorula infections have been associated with the presence of central venous catheter (CVC), immunosuppression, exposure to antifungals and the presence of either solid or haematologic malignancies. However, in this latter setting, only a few cases have so far been reported. OBJECTIVES: We have conducted a survey for Rhodotorula infections in haematologic patients. METHODS: Patients' clinical and microbiological data were collected and correlated to the outcome. RESULTS: A total of 27 cases were detected from 13 tertiary care hospitals. About 78% and 89% of patients had acute leukaemia and CVC. About 70% of patients were exposed to prophylaxis with azoles, mainly posaconazole (37%), 59% were severely neutropenic and 37% underwent allogeneic stem cell transplantation (alloSCT). The most frequent treatments were liposomal amphotericin B (L-AmB) and CVC removal in 17 and 16 patients, respectively. One month post-diagnosis, mortality was 26% and was associated with the presence of mucositis (P = 0.034). CONCLUSIONS: Our study shows that Rhodotorula spp should be considered as aetiologic agents of breakthrough infections in acute leukaemia patients with a CVC, mucositis, who receive prophylaxis with azoles, including posaconazole, and/or undergo alloSCT. Prompt measures, such as L-AmB administration and CVC removal, should be carried out to avoid the high mortality risk of Rhodotorula infections.


Assuntos
Antifúngicos/uso terapêutico , Neoplasias Hematológicas/complicações , Micoses/tratamento farmacológico , Micoses/epidemiologia , Rhodotorula/isolamento & purificação , Adolescente , Adulto , Idoso , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Micoses/microbiologia , Micoses/mortalidade , Prevalência , Estudos Retrospectivos , Fatores de Risco , Análise de Sobrevida , Centros de Atenção Terciária , Resultado do Tratamento , Adulto Jovem
6.
Microbiologyopen ; 8(3): e00657, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-29926536

RESUMO

Heavy metal pollution in Antarctic is serious by anthropogenic emissions and atmospheric transport. To dissect the heavy metal adaptation mechanisms of sea-ice organisms, a basidiomycetous yeast strain AN5 was isolated and its cellular changes were analyzed. Morphological, physiological, and biochemical characterization indicated that this yeast strain belonged to Rhodotorula mucilaginosa AN5. Heavy metal resistance pattern of Cd > Pb = Mn > Cu > Cr > Hg was observed. Scanning electron microscopic (SEM) results exhibited altered cell surface morphology under the influence of copper metal compared to that with control. The determination of physiological and biochemical changes manifested that progressive copper treatment significantly increased antioxidative reagents content and enzymes activity in the red yeast, which quench the active oxygen species to maintain the intercellular balance of redox state and ensure the cellular fission and growth. Comparative proteomic analysis revealed that, under 2 mM copper stress, 95 protein spots were tested reproducible changes of at least 10-fold in cells. Among 95 protein spots, 43 were elevated and 52 were decreased synthesis. After MALDI TOF MS/MS analysis, 51 differentially expressed proteins were identified successfully and classified into six functional groups, including carbohydrate and energy metabolism, nucleotide and protein metabolism, protein folding, antioxidant system, signaling, and unknown function proteins. Function analysis indicated that carbohydrate and energy metabolism-, nucleotide and protein metabolism-, and protein folding-related proteins played central role to the heavy metal resistance of Antarctic yeast. Generally, the results revealed that the yeast has a great capability to cope with heavy metal stress and activate the physiological and protein mechanisms, which allow more efficient recovery after copper stress. Our studies increase understanding of the molecular resistance mechanism of polar yeast to heavy metal, which will be benefitted for the sea-ice isolates to be a potential candidate for bioremediation of metal-contaminated environments.


Assuntos
Cobre/toxicidade , Camada de Gelo/microbiologia , Rhodotorula/efeitos dos fármacos , Rhodotorula/isolamento & purificação , Estresse Fisiológico , Oligoelementos/toxicidade , Regiões Antárticas , Antioxidantes/metabolismo , Farmacorresistência Fúngica , Microscopia Eletrônica de Varredura , Oxirredução , Proteoma/análise , Rhodotorula/química , Rhodotorula/ultraestrutura , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Propriedades de Superfície
7.
PLoS One ; 13(10): e0205891, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30379951

RESUMO

Aconitum carmichaelii, commonly known as Fuzi, is a typical traditional Chinese medicine (TCM) herb that has been grown for more than one thousand years in China. Although root rot disease has been seriously threatening this crop in recent years, few studies have investigated root rot disease in Fuzi, and no pathogens have been identified. In this study, fungal libraries from rhizosphere soils were constructed by internal transcribed spacer (ITS) sequencing using the HiSeq 2500 high-throughput platform. A total of 948,843 tags were obtained from 17 soil samples, and these corresponded to 195,583,495 nt. At 97% identity, the libraries yielded 12,266 operational taxonomic units (OTUs), of which 97.5% could be annotated. In sick soils, Athelia, Mucor and Mortierella were the dominant fungi, comprising 10.3%, 10.1% and 7.7% of the fungal community, respectively. These fungi showed 2.6-, 1.53- to 6.31- and 1.38- to 2.65-fold higher enrichment in sick soils compared with healthy soils, and their high densities reduced the fungal richness in the areas surrounding the rotted Fuzi roots. An abundance analysis suggested that A. rolfsii and Mucor racemosus, as the dominant pathogens, might play important roles in the invading Fuzi tissue, and Phoma adonidicola could be another pathogenic fungus of root rot. In contrast, Mortierella chlamydospora, Penicillium simplicissimum, Epicoccum nigrum, Cyberlindnera saturnus and Rhodotorula ingeniosa might antagonize root rot pathogens in sick soils. In addition, A. rolfsii was further verified as a main pathogen of Fuzi root rot disease through hypha purification, morphological observation, molecular identification and an infection test. These results provide theoretical guidance for the prevention and treatment of Fuzi root rot disease.


Assuntos
Aconitum/microbiologia , DNA Fúngico/genética , DNA Intergênico/genética , Fungos/genética , Raízes de Plantas/microbiologia , Microbiologia do Solo , Biodiversidade , Etiquetas de Sequências Expressas , Fungos/classificação , Fungos/isolamento & purificação , Fungos/patogenicidade , Biblioteca Genômica , Sequenciamento de Nucleotídeos em Larga Escala , Mortierella/classificação , Mortierella/genética , Mortierella/isolamento & purificação , Mortierella/patogenicidade , Penicillium/classificação , Penicillium/genética , Penicillium/isolamento & purificação , Penicillium/patogenicidade , Filogenia , Doenças das Plantas/microbiologia , Rizosfera , Rhodotorula/classificação , Rhodotorula/genética , Rhodotorula/isolamento & purificação , Rhodotorula/patogenicidade , Saccharomycetales/classificação , Saccharomycetales/genética , Saccharomycetales/isolamento & purificação , Saccharomycetales/patogenicidade , Solo/química
8.
World J Microbiol Biotechnol ; 34(9): 127, 2018 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-30084085

RESUMO

Silver nanoparticles (AgNPs) have several technological applications and may be synthetized by chemical, physical and biological methods. Biosynthesis using fungi has a wide enzymatic range and it is easy to handle. However, there are few reports of yeasts with biosynthetic ability to produce stable AgNPs. The purpose of this study was to isolate and identify soil yeasts (Rhodotorula glutinis and Rhodotorula mucilaginosa). After this step, the yeasts were used to obtain AgNPs with catalytic and antifungal activity evaluation. Silver Nanoparticles were characterized by UV-Vis, DLS, FTIR, XRD, EDX, SEM, TEM and AFM. The AgNPs produced by R. glutinis and R. mucilaginosa have 15.45 ± 7.94 nm and 13.70 ± 8.21 nm (average ± SD), respectively, when analyzed by TEM. AgNPs showed high catalytic capacity in the degradation of 4-nitrophenol and methylene blue. In addition, AgNPs showed high antifungal activity against Candida parapsilosis and increase the activity of fluconazole (42.2% for R. glutinis and 29.7% for R. mucilaginosa), while the cytotoxicity of AgNPs was only observed at high concentrations. Finally, two yeasts with the ability to produce AgNPs were described and these particles showed multifunctionality and can represent a technological alternative in many different areas with potential applications.


Assuntos
Antifúngicos/farmacologia , Nanopartículas Metálicas/química , Rhodotorula/isolamento & purificação , Rhodotorula/metabolismo , Prata/química , Antibacterianos/farmacologia , Antifúngicos/química , Brasil , Candida parapsilosis/efeitos dos fármacos , Catálise , Linhagem Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Combinação de Medicamentos , Sinergismo Farmacológico , Fluconazol/farmacologia , Humanos , Nanopartículas Metálicas/ultraestrutura , Azul de Metileno/metabolismo , Testes de Sensibilidade Microbiana , Nitrofenóis/metabolismo , Tamanho da Partícula , Filogenia , Rhodotorula/classificação , Microbiologia do Solo , Águas Residuárias , Purificação da Água
9.
Aust Vet J ; 96(8): 297-301, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30129035

RESUMO

BACKGROUND: Mycotic rhinitis is a rare disease in horses, with few cases reported worldwide and none reported in Australia. Fungal infection of the upper respiratory tract can occur in all species, but its prevalence in horses is considerably lower than in canines or humans. The disease is linked to a variety of pathogens and the clinical signs are associated with subsequent upper respiratory tract damage. CASE REPORT: A 6-year-old Quarter Horse gelding in Queensland, Australia, was presented with chronic nasal discharge and dyspnoea following previous unsuccessful medical treatment of 9 months' duration. Culture yielded a mixed growth of Cryptococcus and Rhodotorula, as well as secondary bacterial species. The horse was anaesthetised and positioned in sternal recumbency. The nasal passages and sinuses were bathed with fluconazole and dimethylsulfoxide for 1 h on two separate occasions 4 weeks apart. Systemic antimicrobials and corticosteroids were also administered for brief courses. Endoscopic examination 4 months after treatment demonstrated clinical resolution of the rhinitis with residual scar tissue. Owners reported resolution of clinical signs and following the treatment the gelding was able to perform athletically. CLINICAL RELEVANCE: Mycotic rhinitis is an unusual disease that can be difficult to treat successfully. This report describes a technique for topical sinonasal bathing under general anaesthetic that was well tolerated, easy to perform and resulted in a successful clinical outcome.


Assuntos
Antifúngicos/administração & dosagem , Doenças dos Cavalos/tratamento farmacológico , Doenças dos Cavalos/microbiologia , Micoses/veterinária , Rinite/veterinária , Anestesia Geral/veterinária , Animais , Cryptococcus/isolamento & purificação , Endoscopia/veterinária , Cavalos , Masculino , Micoses/tratamento farmacológico , Queensland , Rinite/tratamento farmacológico , Rinite/microbiologia , Rhodotorula/isolamento & purificação , Resultado do Tratamento
10.
J Mycol Med ; 28(2): 393-395, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29661607

RESUMO

Rhodotorula species are environmental basidiomycete yeasts that have emerged as a cause of fungemia in immunocompromised hosts. The insertion of a central venous catheter was identified as a major risk factor for Rhodotorula fungemia. Few cases reports have reported (1→3)-ß-D-glucan testing at the onset of Rhodotorula mucilaginosa fungemia. We report a case of catheter-related bloodstream infection due to R. mucilaginosa. Serum ß-D-glucan level was normal at the onset of the bloodstream infection. It took 5 days to culture the isolate. The patient's fever persisted after empiric treatment with micafungin, and a switch to oral voriconazole immediately resolved the fungemia.


Assuntos
Infecções Relacionadas a Cateter/sangue , Infecções Relacionadas a Cateter/microbiologia , Fungemia/microbiologia , Glucanos/sangue , Rhodotorula/isolamento & purificação , Idoso , Antifúngicos/uso terapêutico , Infecções Relacionadas a Cateter/tratamento farmacológico , Feminino , Febre , Fungemia/diagnóstico , Fungemia/tratamento farmacológico , Humanos , Hospedeiro Imunocomprometido , Micafungina/uso terapêutico , Rhodotorula/efeitos dos fármacos , Fatores de Risco , Voriconazol/uso terapêutico
11.
J Basic Microbiol ; 58(4): 331-342, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29442377

RESUMO

Psychrotolerant yeast Rhodotorula sp. Y-23 was isolated from the sediment core sub-samples of Nella Lake, East Antarctica. Isolate was screened for lipase production using plate assay method followed by submerged fermentation. Production optimization revealed the maximum lipase production by using palmolein oil (5% v/v), pH 8.0 and inoculum size of 2.5% v/v at 15 °C. The potential inducers for lipase were 1% w/v of galactose and KNO3 , and MnCl2 (0.1% w/v). Final productions with optimized conditions gave 5.47-fold increase in lipase production. Dialyzed product gave a purification fold of 5.63 with specific activity of 26.83 U mg-1 and 15.67% yields. This lipase was more stable at pH 5.0 and -20 °C whereas more activity was found at pH 8.0 and 35 °C. Stability was more in 50 mM Fe3+ , EDTA-Na (20 mM), sodium deoxycholate (20 mM), H2 O2 (1% v/v), and almost all organic solvents (50% v/v). Tolerance capacity at wider range of pH and temperature with having lower Km value i.e., 0.08 mg ml-1 and higher Vmax 385.68 U mg-1 at 15 °C make the studied lipase useful for industrial applications. Besides this, the lipase was compatible with commercially available detergents, and its addition to them increases lipid degradation performances making it a potential candidate in detergent formulation.


Assuntos
Temperatura Baixa , Proteínas Fúngicas/metabolismo , Sedimentos Geológicos/microbiologia , Lipase/metabolismo , Rhodotorula/enzimologia , Solventes/farmacologia , Regiões Antárticas , Detergentes/química , Estabilidade Enzimática/efeitos dos fármacos , Proteínas Fúngicas/biossíntese , Proteínas Fúngicas/isolamento & purificação , Proteínas Fúngicas/fisiologia , Concentração de Íons de Hidrogênio , Cinética , Lipase/biossíntese , Lipase/isolamento & purificação , Lipase/fisiologia , Óleos Vegetais/metabolismo , Rhodotorula/classificação , Rhodotorula/genética , Rhodotorula/isolamento & purificação , Especificidade por Substrato , Temperatura
12.
Perit Dial Int ; 38(1): 69-73, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29311198

RESUMO

Rhodotorula mucilaginosa is a ubiquitous yeast that may cause serious peritoneal dialysis (PD) infections. A 70-year-old man receiving continuous ambulatory peritoneal dialysis (CAPD) for 6 months presented with a PD infection caused by Rhodotorula mucilaginosa The patient was treated with intravenous liposomal amphotericin B. His peritoneal catheter was simultaneously removed and reinserted. To date, only 10 cases of Rhodotorula CAPD infections have been reported. Catheters, corticoids, cancer, and previous antibiotic therapy were the main risk factors for these infections. For most patients, the peritoneal catheter was removed, leading to a definitive transfer to hemodialysis. Along with flucytosine, amphotericin B is the main antifungal that is used. Both have a lower minimal inhibitory concentration (MIC), whereas fluconazole and caspofungin have the highest MICs. We highlighted the efficacy of liposomal amphotericin B associated with simultaneous withdrawal and reinsertion of a peritoneal catheter in a case of Rhodotorula peritonitis in a CAPD patient. Further studies are needed to evaluate the treatment of this condition.


Assuntos
Anfotericina B/uso terapêutico , Antifúngicos/uso terapêutico , Micoses/diagnóstico , Diálise Peritoneal Ambulatorial Contínua/efeitos adversos , Peritonite/microbiologia , Idoso , Humanos , Masculino , Micoses/tratamento farmacológico , Peritonite/tratamento farmacológico , Rhodotorula/isolamento & purificação
13.
Mycopathologia ; 183(3): 637-644, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29196923

RESUMO

This report describes the isolation of two environmental fungi: Rhodotorula mucilaginosa and Trichosporon jirovecii accompanied by Pseudomonas aeruginosa and Escherichia coli from a dog with bronchotracheitis. All microorganisms were isolated routinely from a mucopurulent discharge, obtained during bronchoscopy from laryngotracheal area. The initial identification of yeasts was confirmed by API Candida and by molecular analysis of internal transcribed spacer region. Administered antimicrobial treatment with Marbofloxacin and Canizol has brought the improvement in the dogs' health status. The final results of control mycological culture were negative. Most probably underlying hypothyroidism and the use of steroids were the factors predisposing this patient to opportunistic infection of mixed aetiology. As far as we are concerned, this is the first case of dogs' respiratory tract infection caused by R. mucilaginosa and T. jirovecii.


Assuntos
Coinfecção/veterinária , Doenças do Cão/diagnóstico , Escherichia coli/isolamento & purificação , Pseudomonas aeruginosa/isolamento & purificação , Infecções Respiratórias/veterinária , Rhodotorula/isolamento & purificação , Trichosporon/isolamento & purificação , Animais , Antibacterianos/administração & dosagem , Antifúngicos/administração & dosagem , Broncoscopia , Análise por Conglomerados , Coinfecção/diagnóstico , Coinfecção/microbiologia , Coinfecção/patologia , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Fúngico/química , DNA Fúngico/genética , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Doenças do Cão/microbiologia , Doenças do Cão/patologia , Cães , Escherichia coli/classificação , Escherichia coli/genética , Feminino , Técnicas Microbiológicas , Filogenia , Pseudomonas aeruginosa/classificação , Pseudomonas aeruginosa/genética , Infecções Respiratórias/diagnóstico , Infecções Respiratórias/microbiologia , Infecções Respiratórias/patologia , Rhodotorula/classificação , Rhodotorula/genética , Análise de Sequência de DNA , Resultado do Tratamento , Trichosporon/classificação , Trichosporon/genética
14.
Biofouling ; 34(1): 74-85, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-29228797

RESUMO

The factors affecting the mechanical properties of biofilms formed by yeast species (Rhodotorula mucilaginosa, Candida krusei, C. kefyr and C. tropicalis) isolated from the juice processing industries have been investigated. Variables studied were: the food matrix (apple/pear juice), the sugar concentration (6/12 °Bx) and the hydrodynamic conditions (static/turbulent flow). A range of environmental cues were included as the mechanical properties of biofilms are complex. Yeast counts were significantly higher in turbulent flow compared with under static conditions. The thickness of the biofilm ranged from 38 to 148 µm, from static to turbulent flow. Yeast biofilms grown under turbulent flow conditions were viscoelastic with a predominant solid-like behavior and were structurally stronger than those grown under static conditions, indicating gel-type structures. Only the type of flow had a significant effect on [Formula: see text] and G*. Flow velocity and nutrient status modulated the biofilm thickness, the biomass and the mechanical properties. A better knowledge of the factors controlling biofilm formation will help in the development of control strategies.


Assuntos
Biofilmes/crescimento & desenvolvimento , Candida/isolamento & purificação , Sucos de Frutas e Vegetais , Rhodotorula/isolamento & purificação , Biomassa , Candida/crescimento & desenvolvimento , Indústria Alimentícia/instrumentação , Microbiologia de Alimentos/normas , Hidrodinâmica , Membranas Artificiais , Reologia , Rhodotorula/crescimento & desenvolvimento , Ultrafiltração/instrumentação
15.
16.
Mycoses ; 61(1): 35-39, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28922488

RESUMO

Rhodotorula species have traditionally been considered as one of common non-virulent environmental inhabitant. They have emerged as an opportunistic pathogen, particularly in immunocompromised hosts and most infections have been associated with intravenous catheters in these patients. We review the isolates in blood cultures of Rhodotorula mucilaginosa in our Hospital. We describe the demographic and clinical features of the cases and the antifungal susceptibility profiles of the isolates. Selected patients had an isolation of R. mucilaginosa in blood cultures in our tertiary care Hospital. All data were collected retrospectively from clinical records during 5 years. We report 8 isolates in blood, two of them were considered contaminants. Immunosuppression, surgery, previous antibiotic therapy were common clinical features. For all the isolates, minimum inhibitory concentration (MIC) values were high for echinocandins and azoles and low for amphotericin B and 5-flucytosine. One strain showed atypical susceptibility profile. Rhodotorula mucilaginosa may be present on the skin and blood cultures can be contaminated. Fungaemia due to R. mucilaginosa is a rare clinical entity which requires risk factors but clinically relevant because of the multiresistant profile. Rhodotorula mucilaginosa shows high MIC values for azoles and echinocandins, therefore amphotericin B and flucytosine must be administered as antifungal therapy.


Assuntos
Antifúngicos/farmacologia , Hemocultura , Fungemia/microbiologia , Hospedeiro Imunocomprometido , Rhodotorula/isolamento & purificação , Idoso , Idoso de 80 Anos ou mais , Anfotericina B/farmacologia , Antifúngicos/uso terapêutico , Azóis/farmacologia , Pré-Escolar , Equinocandinas/farmacologia , Feminino , Flucitosina/farmacologia , Fungemia/tratamento farmacológico , Humanos , Lactente , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Estudos Retrospectivos , Rhodotorula/efeitos dos fármacos , Rhodotorula/crescimento & desenvolvimento , Espanha , Centros de Atenção Terciária
17.
Anal Chim Acta ; 990: 185-193, 2017 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-29029742

RESUMO

Pre-separation and pre-concentration of bacteria is an important step especially when they are uncultured and bacterial concentration in the matrix is low. This study describes a preparative method based on isoelectric focusing of colored microorganisms in a cellulose-based separation medium from a high conductivity matrix. The isoelectric points found for the examined cells were 1.8 for Micrococcus luteus, 3.5 for Dietzia sp., and 4.7 for Rhodotorula mucilaginosa using capillary isoelectric focusing. The final positions of the zones of colored microbial cells in the cellulose-bed are indicated by colored pI markers. Segments of the separation medium with cells were harvested by a spatula, simply purified using centrifugation and analyzed by capillary isoelectric focusing and matrix-assisted laser desorption/ionization time of flight mass spectrometry. The determined recovery ranged from 78% to 93%. The viability of the harvested cells was verified by their cultivation.


Assuntos
Actinobacteria/isolamento & purificação , Celulose/química , Focalização Isoelétrica , Micrococcus/isolamento & purificação , Rhodotorula/isolamento & purificação , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
18.
PLoS One ; 12(8): e0182533, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28771577

RESUMO

Feed security, feed quality and issues surrounding the safety of raw materials are always of interest to all livestock farmers, feed manufacturers and competent authorities. These concerns are even more important when alternative feed ingredients, new product developments and innovative feeding trends, like insect-meals, are considered. The black soldier fly (Hermetia illucens) is considered a good candidate to be used as feed ingredient for aquaculture and other farm animals, mainly as an alternative protein source. Data on transfer of contaminants from different substrates to the insects, as well as the possible occurrence of toxin-producing fungi in the gut of non-processed insects are very limited. Accordingly, we investigated the impact of the substrate/diet on the intestinal mycobiota of H. illucens larvae using culture-dependent approaches (microbiological analyses, molecular identification through the typing of isolates and the sequencing of the 26S rRNA D1/D2 domain) and amplicon-based next-generation sequencing (454 pyrosequencing). We fed five groups of H. illucens larvae at the third growing stage on two substrates: chicken feed and/or vegetable waste, provided at different timings. The obtained results indicated that Pichia was the most abundant genus associated with the larvae fed on vegetable waste, whereas Trichosporon, Rhodotorula and Geotrichum were the most abundant genera in the larvae fed on chicken feed only. Differences in the fungal communities were highlighted, suggesting that the type of substrate selects diverse yeast and mold genera, in particular vegetable waste is associated with a greater diversity of fungal species compared to chicken feed only. A further confirmation of the significant influence of diet on the mycobiota is the fact that no operational taxonomic unit common to all groups of larvae was detected. Finally, the killer phenotype of isolated yeasts was tested, showing the inhibitory activity of just one species against sensitive strains, out of the 11 tested species.


Assuntos
Ração Animal/microbiologia , Fungos/classificação , Análise de Sequência de DNA/métodos , Simuliidae/fisiologia , Animais , DNA Fúngico/análise , DNA Ribossômico/análise , Microbiologia de Alimentos , Fungos/genética , Fungos/isolamento & purificação , Microbioma Gastrointestinal , Larva/microbiologia , Pichia/classificação , Pichia/isolamento & purificação , RNA Ribossômico/análise , Rhodotorula/classificação , Rhodotorula/isolamento & purificação , Simuliidae/microbiologia , Trichosporon/classificação , Trichosporon/isolamento & purificação
19.
ScientificWorldJournal ; 2017: 2982478, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28695189

RESUMO

Fungi can cause various diseases, and some pathogenic fungi have been detected in the water of dental equipment. This environment offers suitable conditions for fungal biofilms to emerge, which can facilitate mycological contamination. This study verified whether the water employed in the dental units of two dental clinics at the University of Franca was contaminated with fungi. This study also evaluated the ability of the detected fungi to form biofilms. The high-revving engine contained the largest average amount of fungi, 14.93 ± 18.18 CFU/mL. The main fungal species verified in this equipment belonged to the genera Aspergillus spp., Fusarium spp., Candida spp., and Rhodotorula spp. Among the isolated filamentous fungi, only one fungus of the genus Fusarium spp. did not form biofilms. As for yeasts, all the Candida spp. isolates grew as biofilm, but none of the Rhodotorula spp. isolates demonstrated this ability. Given that professionals and patients are often exposed to water and aerosols generated by the dental procedure, the several fungal species detected herein represent a potential risk especially to immunocompromised patients undergoing dental treatment. Therefore, frequent microbiological monitoring of the water employed in dental equipment is crucial to reduce the presence of contaminants.


Assuntos
Equipamentos Odontológicos/microbiologia , Contaminação de Equipamentos , Microbiologia da Água , Aspergillus/isolamento & purificação , Candida/isolamento & purificação , Fusarium/isolamento & purificação , Humanos , Micoses/microbiologia , Rhodotorula/isolamento & purificação
20.
Mycoses ; 60(11): 736-738, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28730647

RESUMO

Environmental conditions for swimming facilities may support fungal growth and also may represent a biological risk for users. However, since previously published studies both cleaning procedures and sanitary regulations are likely to have been improved. The aim of the study was to examine whether attendance to public swimming pools was still a risk factor for fungal infection of the skin in Poland. We investigated the occurrence of mycotic species, in a sample from Polish swimming pools. Detection and identification of isolated species were achieved by cultural and morphological methods. Study revealed high incidence of yeast and yeast-like fungi. Candida spp. and Rhodotorula rubra were commonly detected, followed by Aspergillus spp. and Trichophyton mentagrophytes. Surprisingly, the prevalence of clinically important species isolated from swimming pool environments did not reflect prevalence of those species in Polish population. The present results are in agreement with previous research and support that swimming pools remain an important reservoir of clinically important fungi, yet the exposure associated with swimming pools may not be a major source of dermatophytic infection in Poland.


Assuntos
Fungos/isolamento & purificação , Micoses/epidemiologia , Piscinas , Candida/isolamento & purificação , Humanos , Micoses/microbiologia , Polônia/epidemiologia , Rhodotorula/isolamento & purificação , Inquéritos e Questionários , Trichophyton/isolamento & purificação , Leveduras/isolamento & purificação
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