Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.617
Filtrar
1.
Rev Bras Parasitol Vet ; 28(4): 728-734, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31721928

RESUMO

Free-ranging and feral dogs represent a group of unattended companion animals. They impact wild animal populations by predating native species, displacing predators and introducing exotic pathogens. The aim of this work was to describe the molecular occurrence of Rickettsia, Ehrlichia, Anaplasma, Mycoplasma and Bartonella in feral dogs. The study was carried out in the last relict of a protected area in Mexico City. Blood clots samples from 19 dogs were obtained and analyzed for detection of specific fragments of the 16S-rRNA gene for Anaplasma, Ehrlichia and Mycoplasma and citrate synthase (gltA) for Bartonella and Rickettsia. Our results showed that DNA from three bacteria species (Bartonella vinsonii subsp. berkhoffii, Ehrlichia canis and Mycoplasma haemocanis) was present with frequencies ranging from 5.3 to 15.8%. This is the first record of B. vinsonii subsp. berkhoffii and M. haemocanis in dogs from México, and also the first finding of Ehrlichia canis in Mexico City. It is important to perform surveillance of feral dog populations in order to identify the impact of these pathogens on wild animal populations and Public Health in order to establish prevention and protection programs.


Assuntos
Anaplasma/isolamento & purificação , Bartonella/isolamento & purificação , Doenças do Cão/microbiologia , Ehrlichia/isolamento & purificação , Infecções por Bactérias Gram-Negativas/veterinária , Mycoplasma/isolamento & purificação , Rickettsia/isolamento & purificação , Anaplasma/genética , Animais , Animais Selvagens , Bartonella/genética , Doenças do Cão/epidemiologia , Cães , Ehrlichia/genética , Feminino , Infecções por Bactérias Gram-Negativas/epidemiologia , Infecções por Bactérias Gram-Negativas/microbiologia , Masculino , México/epidemiologia , Mycoplasma/genética , Filogenia , Rickettsia/genética
2.
Rev Bras Parasitol Vet ; 28(4): 713-721, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31721931

RESUMO

Rickettsia spp. bacteria are responsible for tick-borne diseases worldwide, mostly maintained by rickettsial amplifiers capybaras in Brazilian endemic areas. The campus of the University of São Paulo, in southeastern Brazil, is an area endemic for Brazilian spotted fever (BSF), with high density of capybaras and Amblyomma spp., along with confirmed human cases. Besides capybaras, the university has also an in-campus high population of sheltered and free-roaming cats. Accordingly, the aim of this study was to determine the prevalence and characteristics associated with Rickettsia rickettsii, Rickettsia parkeri and Rickettsia felis exposure among cats in a BSF-endemic area. Out of 51 cats sampled, 23/35 shelter (65.7%) and 5/16 free-roaming (31.2%) were positive (titers ≥ 64) for at least one Rickettsia species. Ticks species were present in 3/16 free-roaming cats (18.8%), consisting of Amblyomma spp., nymphs of Amblyomma sculptum and adult Rhipicephalus sanguineus sensu lato. Despite sharing the capybaras environment, the seropositivity among the free-roaming and shelter cats was lower than owned cats in other endemic areas. Whether equally or less exposed to rickettsial infection, compared with owned cats in endemic areas, free-roaming and shelter cats may be used as environmental sentinels for human exposure to rickettsiae in such areas.


Assuntos
Doenças do Gato/microbiologia , Infecções por Rickettsia/veterinária , Rickettsia/isolamento & purificação , Carrapatos/microbiologia , Animais , Brasil/epidemiologia , Doenças do Gato/diagnóstico , Doenças do Gato/epidemiologia , Gatos , Feminino , Masculino , Rickettsia/classificação , Infecções por Rickettsia/diagnóstico , Infecções por Rickettsia/epidemiologia , Infecções por Rickettsia/microbiologia , Febre Maculosa das Montanhas Rochosas/transmissão
3.
Zhonghua Yu Fang Yi Xue Za Zhi ; 53(11): 1130-1135, 2019 Nov 06.
Artigo em Chinês | MEDLINE | ID: mdl-31683400

RESUMO

Objective: To understand the situation and genotype distribution of spotted fever group rickettsia (SFGR) in the border area of Tumen River Basin in free ticks in Yanbian Korean Autonomous Prefecture (Yanbian Prefecture), Jilin Province. Methods: From April to September, 2017, ticks were collected using flagging method from Hunchun, Tumen, Helong and Longjing cities in the Tumen River basin of Yanbian Prefecture. Outer membrane protein A (ompA) was detected by Polymerase Chain Reaction (PCR), then, the species were identified by gene sequencing and analyzed systematically. The positive rate of pools and MIR(minimum infection rate per 100 ticks,MIR) of SFGR were calculated, and the difference of positive rate of pools among ticks with different characteristics was compared by Chi-square test. Results: A total of 3 079 ticks were collected and divided into 536 pools. The positive rate of pools of SFGR nucleic acid was 39.7% (213 pools). The MIR of SFGR was 6.9%.The positive rate of pools of SFGR in Dermacentor silvarum, Haemaphysalis concinna, Haemaphysalis japonica, Haemaphysalis longicornis and Ixodes persulcatus were 80.4% (41/51), 14.0% (25/179), 20.2% (18/89), 78.9% (101/128) and 25.9% (21/81), and the difference was statistically significant (P<0.001). There was statistical difference in the positive rate of pools of SFGR in developmental stages of ticks (P<0.001); the positive rate of pools of female adults, male adults, nymph and larvae were 36.4% (95/261), 34.2% (67/196), 56.3% (40/71) and 7/8, and the MIR was 7.9%, 7.7%, 4.9% and 3.5%. The five genotype was detected which was Candidatus Rickettsia longicornii, Rickettsia raoultii, Rickettsia heilongjiangensis, Candidatus Rickettsia tarasevichiae,Rickettsia monacensis and have 98%-100% homology with known gene sequences. Candidatus Rickettsia longicornii, Rickettsia raoultii, Rickettsia heilongjiangensis and Candidatus Rickettsia tarasevichiae showed close evolutionary relationship with known specie (have 98%-100% homology with known gene sequences); Rickettsia monacensis showed Far from evolutionary relationship with known species (have 98% homology with known gene sequences). Conclusion: SFGR infection of ticks is common in the border areas of the Tumen River Basin. There was high diversity in SFGR species and tick species in the areas surveyed.


Assuntos
Proteínas da Membrana Bacteriana Externa/genética , Ixodidae/microbiologia , Rickettsia/classificação , Rickettsia/isolamento & purificação , Rickettsiose do Grupo da Febre Maculosa/diagnóstico , Carrapatos , Animais , China , Feminino , Ixodidae/classificação , Ixodidae/crescimento & desenvolvimento , Masculino , Reação em Cadeia da Polimerase , Rickettsia/genética , Rios , Análise de Sequência
4.
Rev Bras Parasitol Vet ; 28(4): 563-568, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31576972

RESUMO

Chiggers are ectoparasites of vertebrates and may cause trombiculiasis or transmit pathogens to their hosts. Specimens collected from rodents and marsupials were morphologically identified as Herpetacarus hertigi, Eutrombicula tinami, Kymocta sp., Quadraseta brasiliensis, Quadraseta falconensis, Quadraseta flochi, Quadraseta mackenziei, Quadraseta pazca, Quadraseta trapezoides, Quadraseta sp., Serratacarus sp., and Trombewingia bakeri. These mites were submitted individually to molecular analyses for the detection of bacteria of the genus Coxiella, Hepatozoon and Rickettsia. Samples were positive to Rickettsia only. Obtained sequences for the gltA (350 pb) and ompA (488 pb) genes were identical to "Candidatus Rickettsia colombianensi", a species previously detected in ticks. In addition, molecular identification of mites based on 18S rDNA sequences are provided for H. hertigi, Kymocta sp., Q. brasiliensis, Q. pazca, Q. trapezoides, Quadraseta sp., and T. bakeri for the first time. This is the first report of the detection of a Rickettsia sp. in chigger mites collected on rodents in Brazil.


Assuntos
Marsupiais/parasitologia , Infestações por Ácaros/veterinária , Rickettsia/genética , Roedores/parasitologia , Trombiculidae/microbiologia , Animais , Reação em Cadeia da Polimerase , RNA Bacteriano/genética , RNA Ribossômico 18S/genética , Rickettsia/isolamento & purificação
5.
Parasit Vectors ; 12(1): 471, 2019 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-31604442

RESUMO

BACKGROUND: Rickettsia parkeri strain Atlantic rainforest has emerged in Brazil during the last 10 years, with three laboratory-confirmed human cases. While these cases were epidemiologically associated with the tick Amblyomma ovale, in none of them the tick specimens that bit the patients could be identified. RESULTS: We report a clinical case of spotted fever rickettsiosis that was acquired in an Atlantic forest area in Bahia state, northeast Brazil. The case was determined to be caused by R. parkeri strain Atlantic rainforest, based on molecular analysis of the crust removed from the tick bite site (inoculation eschar) of the patients' skin. DNA extracted from the crust yielded partial sequences of three rickettsial genes (gltA, ompA and ompB), which were 99-100% identical to R. parkeri strain Atlantic rainforest. The tick specimen that was attached to patient skin was identified as a female of A. ovale. CONCLUSIONS: We report the fourth confirmed case of spotted fever rickettsiosis caused by R. parkeri strain Atlantic rainforest, providing to our knowledge for the first time, direct evidence of R. parkeri strain Atlantic rainforest transmission by A. ovale.


Assuntos
Rickettsia/genética , Febre Maculosa das Montanhas Rochosas/etiologia , Picadas de Carrapatos/complicações , Adulto , Dorso , Brasil , DNA Bacteriano/genética , DNA Bacteriano/isolamento & purificação , Feminino , Humanos , Floresta Úmida , Rickettsia/isolamento & purificação , Febre Maculosa das Montanhas Rochosas/diagnóstico
6.
Ticks Tick Borne Dis ; 10(6): 101278, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31477530

RESUMO

North Asian tick-typhus (NATT), also known as Siberian tick typhus, is the main tick-borne rickettsiosis in Siberia, Russia. Recently, a fatal infection in a four-year-old girl with typical tick-borne rickettsiosis symptoms (fever, rash, eschar at the site of the tick bite, myalgia) and meningeal syndrome was registered. In order to identify the etiology of this infection, blood and brain samples from the patient were examined for the presence of a wide range of tick-transmitted agents and enteric viruses by polymerase chain reaction with subsequent sequencing. Rickettsia sibirica subsp. sibirica and "Candidatus Rickettsia tarasevichiae" DNA was identified in both blood and brain samples. Rickettsia sibirica was characterized by the gltA, ompA and ompB genes, and "Candidatus R. tarasevichiae" was characterized by the gltA and ompB genes. We report the first case of mixed R. sibirica and "Candidatus R. tarasevichiae" human infection with a fatal outcome in Russia.


Assuntos
Coinfecção/microbiologia , Rickettsia/isolamento & purificação , Rickettsiose do Grupo da Febre Maculosa/microbiologia , Doenças Transmitidas por Carrapatos/microbiologia , Pré-Escolar , Coinfecção/diagnóstico , Evolução Fatal , Feminino , Humanos , Sibéria , Rickettsiose do Grupo da Febre Maculosa/diagnóstico , Doenças Transmitidas por Carrapatos/diagnóstico
7.
Parasitol Res ; 118(11): 3185-3189, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31473856

RESUMO

A total of 482 bats representing 32 species and two families were captured in the Amazon forests of the Amapá state in northern Brazil. Nineteen Artibeus planirostris bats (3.9 %) were infested with 160 ticks, all identified as Ornithodoros hasei. Three pools of larvae were screened for rickettsial DNA via polymerase chain reaction (PCR) targeting three rickettsial genes: gltA, ompA and htrA. Only one of them yielded an amplicons of the expected size for all three molecular assays. Comparisons of the obtained sequences including a phylogenetic analysis confirmed the occurrence of "Candidatus Rickettsia wissemanii" in Brazil.


Assuntos
Quirópteros/microbiologia , Quirópteros/parasitologia , Ornithodoros/microbiologia , Infecções por Rickettsia/epidemiologia , Rickettsia/isolamento & purificação , Animais , Proteínas da Membrana Bacteriana Externa/genética , Brasil/epidemiologia , DNA Bacteriano/genética , Proteínas de Choque Térmico/genética , Ixodidae/microbiologia , Larva/microbiologia , Proteínas Periplásmicas/genética , Filogenia , Reação em Cadeia da Polimerase , Rickettsia/genética , Serina Endopeptidases/genética
8.
Med. clín (Ed. impr.) ; 153(5): 205-212, sept. 2019. tab
Artigo em Espanhol | IBECS | ID: ibc-183997

RESUMO

El incremento de los viajes internacionales, la creciente presencia de vectores transmisores de arbovirus en nuestro país, las alertas de fiebres hemorrágicas, como el actual brote de ébola en la R. D. del Congo y los casos autóctonos de fiebre hemorrágica de Crimea-Congo en nuestro país, ponen de nuevo en primer plano las enfermedades tropicales. El aislamiento de los casos sospechosos de enfermedades de alta transmisibilidad y letalidad ha de ser una prioridad (fiebres hemorrágicas, MERS-CoV). Al valorar al paciente, una cuidadosa historia clínica basada en los aspectos epidemiológicos de la zona de procedencia, las actividades realizadas, el tiempo de estancia en el mismo y el inicio de los síntomas nos ayudarán finalmente, si no al diagnóstico definitivo, sí al menos a descartar las enfermedades que signifiquen una amenaza para él. Por su frecuencia y gravedad la malaria debe ser descartada, sin olvidar las otras causas habituales de fiebre con las que el médico de urgencias debe estar familiarizado también


The increase in international travel, the growing presence of arbovirus vectors in our country, and notifications of haemorrhagic fever such as the current outbreak of Ebola in D.R. Congo and the cases of Crimea-Congo haemorrhagic fever in our country have again cast the spotlight on tropical diseases Isolating suspected cases of highly contagious and lethal diseases must be a priority (Haemorrhagic fever, MERS-CoV). Assessing the patient, taking a careful medical history based on epidemiological aspects of the area of origin, activities they have carried out, their length of stay in the area and the onset of symptoms, will eventually help us, if not to make a definitive diagnosis, at least to exclude diseases that pose a threat to these patients. Malaria should be ruled out because of its frequency, without forgetting other common causes of fever familiar to emergency doctors


Assuntos
Humanos , Controle Sanitário de Viajantes , Doença Relacionada a Viagens , Isolamento de Pacientes/tendências , Saúde do Viajante , Doenças Transmissíveis Importadas/epidemiologia , Febres Hemorrágicas Virais/epidemiologia , Infecções por Arbovirus/epidemiologia , Arbovirus/isolamento & purificação , Meningite/epidemiologia , Febre Tifoide/epidemiologia , Rickettsia/isolamento & purificação , Coronavirus/isolamento & purificação , Esquistossomose/epidemiologia
9.
Ticks Tick Borne Dis ; 10(6): 101266, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31402227

RESUMO

The bacterium Rickettsia bellii has been detected in 25 species of ticks in the American continents, but its pathogenic potential is considered as undetermined. A possible role for this species in the phenomenon of transovarial exclusion of pathogenic members of the spotted fever group (SFG) of Rickettsia has been suggested and co-infections with pathogenic species have been reported infrequently in both North and South America. Traditional methods for the molecular detection of rickettsial agents in ticks focus largely on the identification of sequences found in SFG Rickettsia, an approach that may overlook the presence of co-infections with R. bellii. Two novel, species-specific polymerase chain reaction (PCR) assays, targeting the genes encoding the surface cell antigen (Sca), autotransporter proteins sca9 and sca14, were developed and validated for the detection of R. bellii using 150 Amblyomma ticks collected from wild birds in Brazil. Co-infection of R. bellii infected ticks was evaluated using a novel PCR assay targeting the ompA sequence characteristic of SFG Rickettsia. Preliminary species-level identification was achieved by restriction fragment length polymorphism (RFLP) analysis and subsequently confirmed by sequencing of amplicons. Nine out of seventy-three Amblyomma longirostre and one of two Amblyomma calcaratum ticks were shown to be co-infected with R. bellii and Rickettsia amblyommatis, while two out of sixty-seven Amblyomma sp. haplotype Nazaré ticks were recorded as co-infected with R. bellii and the Rickettsia parkeri-like bacterium, strain ApPR. Interestingly, our data represent the first records of R. bellii in association with A. calcaratum and Amblyomma sp. haplotype Nazaré. The novel PCR-RFLP systems reported herein, provide an alternative, rapid and cost-efficient (relative to strategies based on sequencing or real-time PCR), approach to evaluate rickettsial co-infection of ticks, a potentially significant phenomenon that has most likely been underestimated to date.


Assuntos
Ixodidae/microbiologia , Infecções por Rickettsia/veterinária , Rickettsia/isolamento & purificação , Animais , Doenças das Aves/diagnóstico , Doenças das Aves/microbiologia , Aves , Brasil , Coinfecção/veterinária , Rickettsia/genética , Infecções por Rickettsia/classificação , Infecções por Rickettsia/diagnóstico , Rickettsiose do Grupo da Febre Maculosa/classificação , Rickettsiose do Grupo da Febre Maculosa/diagnóstico , Rickettsiose do Grupo da Febre Maculosa/veterinária , Infestações por Carrapato/parasitologia , Infestações por Carrapato/veterinária
10.
Emerg Infect Dis ; 25(9): 1719-1722, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31441748

RESUMO

During 2014-2017, we screened for Rickettsia japonica infection in Xinyang, China, and identified 20 cases. The major clinical manifestations of monoinfection were fever, asthenia, myalgia, rash, and anorexia; laboratory findings included thrombocytopenia and elevated hepatic aminotransferase concentrations. Physicians in China should consider R. japonica infection in at-risk patients.


Assuntos
Infecções por Rickettsia/epidemiologia , Rickettsia/isolamento & purificação , Picadas de Carrapatos , Adulto , Idoso , Animais , China/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Filogenia , Rickettsia/genética , Infecções por Rickettsia/etiologia , Fatores de Risco , Carrapatos
11.
Parasitol Res ; 118(9): 2735-2740, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31375956

RESUMO

Knowledge about vector-borne pathogens important for human and veterinary medicine in wild ruminants in Tyrol (Austria) is scarce. Blood samples from Alpine ibex (Capra ibex; n = 44), Alpine chamois (Rupicapra rupicapra; n = 21), roe deer (Capreolus capreolus; n = 18) and red deer (Cervus elaphus; n = 6) were collected over a period of 4 years (2015-2018) in four regions in North Tyrol, with a primary focus on the Kaunertal. Blood spots on filter paper were tested for the presence of DNA of vector-borne pathogens (Anaplasmataceae, Piroplasmida, Rickettsia and filarioid helminths). Anaplasma phagocytophilum and Babesia capreoli were detected in two of 89 (2.3%) blood samples. Rickettsia spp., Theileria spp. and filarioid helminths were not documented. One Alpine chamois was positive for A. phagocytophilum and B. capreoli. Moreover, an ibex from the Kaunertal region was positive for A. phagocytophilum. While the ibex was a kid less than 1 year old, the chamois was an adult individual. Further research is recommended to evaluate effects of climate change on infection rates of North Tyrolean wild ruminants by these pathogens and the distribution of their vectors.


Assuntos
Anaplasma phagocytophilum/isolamento & purificação , Animais Selvagens/microbiologia , Animais Selvagens/parasitologia , Babesia/isolamento & purificação , Rickettsia/isolamento & purificação , Ruminantes/microbiologia , Ruminantes/parasitologia , Theileria/isolamento & purificação , Anaplasma phagocytophilum/classificação , Anaplasma phagocytophilum/genética , Animais , Áustria , Babesia/classificação , Babesia/genética , Cervos/microbiologia , Cervos/parasitologia , Vetores de Doenças , Cabras/microbiologia , Cabras/parasitologia , Rickettsia/classificação , Rickettsia/genética , Rupicapra/microbiologia , Rupicapra/parasitologia , Theileria/classificação , Theileria/genética
12.
Adv Clin Exp Med ; 28(7): 937-943, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31374162

RESUMO

BACKGROUND: Infections caused by tick-borne pathogens such as Bartonella spp., Borrelia burgdorferi s.l., Coxiella burnetii, and Rickettsia spp. are capable of causing serious lesions of the mitral and aortic valves, leading to a need for valve replacement. OBJECTIVES: The aim of the study was to determine whether such cases are sporadic or frequent. An additional goal was to establish effective diagnostic methods to detect these infections. MATERIAL AND METHODS: The study involved 148 patients undergoing valve replacement. Blood samples were drawn for serological testing. Samples of the removed mitral and aortic valves were tested with polymerase chain reaction and immunohistochemical staining. RESULTS: Specific antibodies to Bartonella spp. were detected in 47 patients (31.7%) and in 1 of the healthy controls (1%) (p < 0.05). Antibodies to B. burgdorferi spirochetes were found in 18 of the patients (12.2%) and in 6 blood donors from the control group (5.8%) (p < 0.1). Antibodies to Rickettsia spp. were detected in 12 (8.1%) and to C. burnetii phase I and II antigens in the serum of 1 patient. All the participants in the control group were seronegative to C. burnetii and Rickettsia spp. antigens. Polymerase chain reaction (PCR) tests for detection of Bartonella spp., B. burgdorferi s.l., C. burnetii and Rickettsia spp. DNA in the valve samples were all negative. Inflammation foci with mononuclear lymphoid cells in the aortic and mitral valves were seen in sections stained with hematoxiline and eozine. In sections dyed using the indirect immunofluorescence method with hyperimmune sera, Bartonella spp. and Rickettsia spp. were found. CONCLUSIONS: The results obtained indicate that laboratory diagnostics for patients with heart disorders should be expanded to include tests detecting tick-borne zoonoses such as bartonelloses, Lyme borreliosis, rickettsioses and Q fever.


Assuntos
Valva Aórtica/microbiologia , Infecções Bacterianas/microbiologia , Bartonella/isolamento & purificação , Borrelia/isolamento & purificação , Coxiella burnetii/isolamento & purificação , Endocardite/microbiologia , Valva Mitral/microbiologia , Rickettsia/isolamento & purificação , Doenças Transmitidas por Carrapatos/complicações , Animais , Bartonella/genética , Borrelia/classificação , Borrelia/genética , Estudos de Casos e Controles , Coxiella burnetii/genética , Endocardite/sangue , Humanos , Reação em Cadeia da Polimerase em Tempo Real , Rickettsia/classificação , Rickettsia/genética , Rickettsiales/classificação , Rickettsiales/genética , Rickettsiales/isolamento & purificação , Doenças Transmitidas por Carrapatos/sangue , Doenças Transmitidas por Carrapatos/diagnóstico , Carrapatos
14.
Ticks Tick Borne Dis ; 10(6): 101254, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31327746

RESUMO

Ixodes persulcatus and Ixodes pavlovskyi ticks, two closely related species of the I. ricinus - I. persulcatus group, are widely distributed in the southern part of Western Siberia. Recently, the existence of natural hybrids of I. persulcatus and I. pavlovskyi ticks has been demonstrated. The aim of this study was to evaluate the abundance of I. persulcatus/pavlovskyi hybrids in several locations with different ratios of parental tick species and to investigate the prevalence and genetic variability of a wide range of infectious agents in these hybrids compared to the parental tick species. Natural hybrids of I. persulcatus and I. pavlovskyi ticks were identified in all examined locations in Altai and Novosibirsk, Western Siberia, Russia. The abundance of hybrids varied from 7% to 40% in different locations and was maximal in a location with similar proportions of I. persulcatus and I. pavlovskyi ticks. For the first time, it was shown that hybrids can be infected with the same agents as their parental tick species: tick-borne encephalitis and Kemerovo viruses, Borrelia afzelii, Borrelia bavariensis, Borrelia garinii, Borrelia miyamotoi, Rickettsia helvetica, Rickettsia raoultii, Rickettsia sibirica, "Candidatus Rickettsia tarasevichiae", Anaplasma phagocytophilum, Ehrlichia muris, "Candidatus Neoehrlichia mikurensis", and Babesia microti. The prevalence of most bacterial agents in hybrids was intermediate compared to their parental tick species. Most genetic variants of the identified agents have been previously found in the parental tick species. Wide distribution of I. persulcatus/pavlovskyi natural hybrids implies that I. persulcatus, I. pavlovskyi and their hybrids coexist in all I. persulcatus - I. pavlovskyi sympatric areas.


Assuntos
Hibridização Genética , Ixodes/microbiologia , Ixodes/parasitologia , Anaplasmataceae/classificação , Anaplasmataceae/isolamento & purificação , Animais , Sequência de Bases , Grupo Borrelia Burgdorferi/classificação , Grupo Borrelia Burgdorferi/isolamento & purificação , Vírus da Encefalite Transmitidos por Carrapatos/classificação , Vírus da Encefalite Transmitidos por Carrapatos/isolamento & purificação , Feminino , Ixodes/genética , Masculino , Orbivirus/classificação , Orbivirus/isolamento & purificação , Filogenia , Rickettsia/classificação , Rickettsia/isolamento & purificação , Alinhamento de Sequência , Sibéria
15.
Ticks Tick Borne Dis ; 10(6): 101263, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31331735

RESUMO

Amblyomma sculptum is the principal vector of Rickettsia rickettsii, the main agent of spotted fever rickettsiosis in Brazil. Little information is available regarding the population dynamics of this tick in some regions, including north-eastern Brazil, where cases of spotted fever rickettsioses are increasingly reported. Herein, we studied the phenology of A. sculptum in a rural area in north-eastern Brazil. Ticks were collected from the environment, using dry ice-baited traps, monthly for two consecutive years. In total, 1500 ticks were collected: 94 females (6.3%), 74 males (4.9%), 468 nymphs (31.2%), and 864 larvae (57.6%). All nymphs and females were identified as A. sculptum. Males were tentatively identified as A. sculptum and larvae as Amblyomma spp. Ticks were more numerous during spring and summer, followed by autumn and winter. Peaks of larvae and nymphs were recorded during summer and spring, respectively, whereas adults were more frequently collected in spring. A total of 380 ticks were tested by PCR for the gltA gene of Rickettsia spp., but none of them were positive. While our results revealed a seasonal pattern for A. sculptum in north-eastern Brazil that is distinct from the seasonal pattern in south-eastern Brazil, we caution that the observed pattern could have been biased by the relatively low number of ticks collected. Finally, the absence of Rickettsia-infected ticks does not rule out the possibility that rickettsial organisms are circulating in the study area and further long-term studies are warranted.


Assuntos
Ixodidae/fisiologia , Floresta Úmida , Animais , Brasil , Feminino , Ixodidae/crescimento & desenvolvimento , Ixodidae/microbiologia , Larva/crescimento & desenvolvimento , Larva/fisiologia , Masculino , Ninfa/crescimento & desenvolvimento , Ninfa/fisiologia , Dinâmica Populacional , Rickettsia/isolamento & purificação
16.
Ticks Tick Borne Dis ; 10(6): 101241, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31279737

RESUMO

Fifty nymphal Ixodes ricinus ticks collected in Alsace, France, identified by morphological criteria and using MALDI-TOF MS, were tested by PCR to detect tick-associated bacteria and protozoan parasites. Seventy percent (35/50) of ticks contained at least one microorganism; 26% (9/35) contained two or more species. Several human pathogens were identified including Borrelia burgdorferi s.s. (4%), Borrelia afzelii (2%), Borrelia garinii (2%), Borrelia valaisiana (4%), Borrelia miyamotoi (2%), Rickettsia helvetica (6%) and "Babesia venatorum" (2%). Bartonella spp. (10%) and a Wolbachia spp. (8%) were also detected. The most common co-infections involved Anaplasmataceae with Borrelia spp. (4%), Anaplasmataceae with Bartonella spp. (6%) and Anaplasmataceae with Rickettsia spp. (6%). Co-infection involving three different groups of bacteria was seen between bacteria of the family Anaplasmataceae, Borrelia spp. and Bartonella spp. (2%). Results highlight the panel of infectious agents carried by Ixodes ricinus. Co-infection suggests the possibility of transmission of more than one pathogen to human and animals during tick blood feeding.


Assuntos
Ixodes/microbiologia , Ixodes/parasitologia , Animais , Babesia/classificação , Babesia/isolamento & purificação , Bartonella/classificação , Bartonella/isolamento & purificação , Borrelia/isolamento & purificação , França , Ixodes/crescimento & desenvolvimento , Ninfa/crescimento & desenvolvimento , Ninfa/microbiologia , Ninfa/parasitologia , Filogenia , Reação em Cadeia da Polimerase , Rickettsia/isolamento & purificação , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Wolbachia/isolamento & purificação
17.
Exp Appl Acarol ; 78(3): 431-442, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31270640

RESUMO

Tick-borne spotted fever in Brazil is known to be caused by two agents, Rickettsia rickettsii and Rickettsia parkeri. Nothing was known about these agents in one area of the Atlantic rainforest biome of Bahia state, where during March to June 2016, 356 rural dogs and 69 horses were sampled and their sera were processed through indirect immunofluorescence assay against antigens of R. rickettsii, R. parkeri, Rickettsia amblyommatis and Rickettsia bellii. Ticks collected from these dogs and horses were molecularly tested for the presence of rickettsial DNA. Overall, 16.4% (58/356) dogs and 24.6% (17/69) horses were seroreactive to Rickettsia spp. Five tick species, Rhipicephalus sanguineus sensu lato (s.l.), Amblyomma ovale, A. sculptum, R. microplus, and A. naponense, were collected from dogs, whereas horses were infested by A. sculptum and Dermacentor nitens. A total of 242 ticks from dogs and 62 from horses were analyzed by PCR targeting rickettsiae, which were detected in only 4/27 (14.8%) A. ovale. Fragments of the rickettsial gltA and ompA genes from these four ticks were 100% identical to the Atlantic rainforest strain of R. parkeri. The presence of A. ovale on dogs was positively associated with local canine seroreactivity to R. parkeri. Our results provide evidence for the transmission of R. parkeri strain Atlantic rainforest from A. ovale to domestic dogs within the rural area of Ilhéus, similarly to other areas of the Atlantic rainforest biome of Brazil, where human cases of R. parkeri-caused spotted fever have been reported.


Assuntos
Doenças do Cão/epidemiologia , Doenças dos Cavalos/epidemiologia , Ixodidae/microbiologia , Infecções por Rickettsia/veterinária , Rickettsia/isolamento & purificação , Infestações por Carrapato/veterinária , Animais , Brasil/epidemiologia , DNA Bacteriano/análise , Doenças do Cão/microbiologia , Doenças do Cão/parasitologia , Cães , Feminino , Doenças dos Cavalos/microbiologia , Doenças dos Cavalos/parasitologia , Cavalos , Ixodidae/crescimento & desenvolvimento , Larva/crescimento & desenvolvimento , Larva/microbiologia , Masculino , Ninfa/crescimento & desenvolvimento , Ninfa/microbiologia , Prevalência , Rhipicephalus/crescimento & desenvolvimento , Rhipicephalus/microbiologia , Infecções por Rickettsia/epidemiologia , Infecções por Rickettsia/microbiologia , Infestações por Carrapato/epidemiologia , Infestações por Carrapato/parasitologia
18.
Ann Ist Super Sanita ; 55(2): 143-150, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31264637

RESUMO

BACKGROUND: A study on tick species characterization and tick borne pathogens detection was performed by a survey conducted during 2012 and 2013 in the Viterbo province (Lazio Region, Central Italy). Seven sites were selected for the study investigation, including two farms and a military zone. METHODS: A total of 255 ticks, Rhipicephalus (Boophilus) annulatus (n = 215), Rhipicephalus bursa (n = 28), and Hyalomma marginatum (n = 12) were screened individually by molecular methods for the tick borne bacterial agents: Borrelia burgdorferi sensu lato group, Bartonella spp., Coxiella burnetii, Ehrlichia spp., Francisella spp., and Rickettsia spp. RESULTS AND CONCLUSION: Overall, 182 ticks (71%) were infected with at least one pathogen; among these co-infections were found in 94 ticks. Tick borne pathogens identified were C. burnetii, B. burgdorferi s.l., Bartonella spp., Rickettsia spp., Francisella spp., and Ehrlichia spp. In R. bursa and H. marginatum, the presence of B. burgdorferi s.l. was positively correlated with that of C. burnetii, Rickettsia spp., and Bartonella spp. and their coinfection probabilities were 29.8%, 22.7% and 11.7%, respectively; the probability of coinfection for Francisella spp. and Rickettsia spp. and for Francisella spp. and Bartonella spp. was 14.9% and 17.9%, respectively. In R. (Boophilus) annulatus, the probability of coinfection between C. burnetii and B. burgdorferi s.l. was 11.3%, while those between C. burnetii and Bartonella spp. and between B. burgdorferi s.l. and Bartonella spp. were 0.8%. Further studies are needed in order to assess the risk associated with these unusual tick-borne pathogens in Central Italy.


Assuntos
Bactérias Gram-Negativas/isolamento & purificação , Ixodidae/microbiologia , Animais , Bartonella/isolamento & purificação , Borrelia burgdorferi/isolamento & purificação , Coxiella burnetii/isolamento & purificação , Ehrlichia/isolamento & purificação , Francisella/isolamento & purificação , Humanos , Itália/epidemiologia , Reação em Cadeia da Polimerase em Tempo Real , Rhipicephalus/microbiologia , Rickettsia/isolamento & purificação , Especificidade da Espécie , Saúde Suburbana , Doenças Transmitidas por Carrapatos/epidemiologia , Doenças Transmitidas por Carrapatos/transmissão
19.
Ticks Tick Borne Dis ; 10(5): 1105-1108, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31201126

RESUMO

The study of rickettsial agents associated with ticks from wild felines is scarce. In Europe, three species of Rickettsia have been detected (Rickettsia helvetica, Rickettsia massiliae, and Rickettsia monacensis) in ticks collected from the Iberian lynx (Lynx pardinus). However, no studies have been conducted on another lynx species. For this reason, the aim of this study was to identify the diversity of Rickettsia species in ticks associated with bobcats (Lynx rufus) collected in the State of Tamaulipas, Mexico. During 1999 and 2004, nine bobcats from two municipalities of the state were trapped and visually inspected for the presence of ticks. A total of 95 ticks were collected from these lynxes. Ticks were preserved in 96% ethanol. Subsequently we identified the presence of Rickettsia DNA by the amplification of several fragments of genes 17 kDa, ompA and ompB. Recovered sequences were concatenated, aligned, and compared with those of reference deposited in GenBank. Additionally, a phylogenetic analysis was performed using the Maximum Likelihood method. The ticks were morphologically identified as belonging to the species Dermacentor variabilis. We selected a subset of 60 ticks which were examined, and 5% (3/60) were positive with an identity of 99% to sequences of R. rickettsii deposited in GenBank. The results obtained represent the first record of R. rickettsii in ticks associated with wild carnivores, and in particular with bobcats distributed in northeast of Mexico.


Assuntos
Dermacentor/microbiologia , Lynx/parasitologia , Rickettsia rickettsii/isolamento & purificação , Animais , Feminino , Masculino , México , Filogenia , Rickettsia/classificação , Rickettsia/isolamento & purificação , Análise de Sequência de DNA/veterinária
20.
Exp Appl Acarol ; 78(3): 403-420, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31165944

RESUMO

In humans, emerging infectious diseases are mostly zoonoses with ticks playing an important role as vectors. Tick-borne relapsing fever Borrelia and spotted fever Rickettsia occur in endemic foci along tropical and subtropical regions of the globe. However, both are widely neglected etiologic agents. In this study, we performed molecular analyses in order to assess the presence of Borrelia and Rickettsia DNA in ticks infesting small-mammals within a National Reserve located in the Andes Mountains, central Chile. While hard ticks were negative for the presence of both agents, sequences of four rickettsial (gltA, htrA, ompA, ompB) and two borrelial (16S rRNA and flaB) genes were obtained from larvae of an Ornithodoros sp. morphologically related with Ornithodoros atacamensis. Phylogenetic analyses indicated that the detected Borrelia and Rickettsia spp. belong to the relapsing fever and spotted fever groups, respectively. Moreover, the agents formed monophyletic clades with Rickettsia amblyommatis and "Candidatus Borrelia johnsonii." As positive ticks parasitize rodents within a highly visited National Reserve where outdoor activities are of common practice, the risk for human parasitism should not be discarded.


Assuntos
Borrelia/isolamento & purificação , Ixodidae/microbiologia , Ornithodoros/microbiologia , Rickettsia/isolamento & purificação , Animais , Borrelia/genética , Chile , Genes Bacterianos , Filogenia , Febre Recorrente , Rickettsia/genética , Análise de Sequência de DNA , Rickettsiose do Grupo da Febre Maculosa
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA